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The photothermal effects of lasers have played an important role in both medical laser applications and the development of cochlear implants with optical stimulation. However, there are few methods to evaluate the thermal effect of micron-sized laser spots interacting with other tissues. Here, we present a multi-wavelength micro-scale laser thermal effect measuring system that has high temporal, spatial and temperature resolutions, and can quantitatively realize evaluations in real time. In this system, with accurate 3D positioning and flexible pulsed laser parameter adjustments, groups of temperature changes are systematically measured when the micron-sized laser spots from six kinds of wavelengths individually irradiate the Pd/Cr thermocouple junction area, and reference data of laser spot thermal effects are obtained. This work develops a stable, reliable and universal tool for quantitatively exploring the thermal effect of micron-sized lasers, and provides basic reference data for research on light-stimulated neuron excitement in the future.

This paper presents an approach to build a novel two-level collocation net, which enables calculation of the collocation relationship between any two words, from a large raw corpus. The first level consists of atomic classes (each atomic class consists of one word and feature bigram), which are clustered into the second level class set. Each class in both levels is represented by its collocation candidate distribution, extracted from the linguistic analysis of the raw training corpus, over possible collocation relation types. In this way, all the information extracted from the linguistic analysis is kept in the collocation net. Our approach applies to both frequently and less-frequently occurring words by providing a clustering mechanism and resolve the data sparseness problem through the collocation net. Experimentation shows that the collocation net is efficient and effective in solving the data sparseness problem and determining the collocation relationship between any two words. [ABSTRACT FROM AUTHOR]

Zeolites X and Y exchanged with Group IA cations were synthesized by aqueous ion exchange of NaX and NaY and used as catalysts in the oxidation of H2 and CO at temperatures ranging from 473 to 573 K. The CsX zeolite was the most active material of the series for both reactions whereas HX was the least active. Moreover, the oxidation of CO in H2 was very selective (∼80%) over the alkali-metal exchanged materials. Isotopic transient analysis of CO oxidation during steady-state reaction at 573 K was used to evaluate the coverage of reactive carbon-containing intermediates that lead to product as well as the pseudo-first-order rate constant of the reaction. A factor of 4 enhancement in activity achieved by exchanging Cs for Na was attributed to a higher coverage of reactive intermediates in CsX because the pseudo-first-order rate constant was nearly same for the two materials (∼0.7 s-1). The number of reactive intermediates on both materials was orders of magnitude below the number of alkali metal cations in the zeolites but was similar to the number of impurity Fe atoms in the samples. Because the trend in Fe impurity loading was the same as that for oxidation activity, a role of transition metal impurities in zeolite oxidation catalysis is suggested. [ABSTRACT FROM AUTHOR]

The mechanism by which high-salt and low-potassium diet contributes to hypertension remains poorly understood. Plasma homocysteine (Hcys) is recognized as a primary mediator of blood pressure (BP) response to some diets. Therefore, the present study tried to investigate whether plasma Hcys and BP could be regulated by salt loading in normotensive salt-sensitive (SS) persons, and further explored whether potassium supplementation could reverse the effect. We enrolled 47 normotensive subjects, aged 29–65 years. The protocol included 7 days on a low-salt diet (3g/day, NaCl), 7 days on a high-salt diet (18g/day), and then a high-salt diet with potassium supplementation (4.5g/day) for 7 days. After high-salt intake, BP was significantly increased and potassium supplementation lowered it in the SS group. Plasma Hcys were higher in SS subjects than in salt-resistant (SR) subjects after salt loading (34.4 ± 17.0 μmol/L versus 19.16 ± 6.4 μmol/L, P < 0.01). Plasma Hcys in SS subjects was increased on a high-salt diet than on a low-salt diet (34.4 ± 17.0 μmol/L versus 16.5 ± 8.3 μmol/L, P < 0.01), but plasma Hcys was ameliorated by potassium supplementation (34.4 ± 17.0 μmol/L versus 20.9 ± 10.4 μmol/L, P < 0.01). In SS subjects, the change of mean arterial blood pressure (MBP) correlated significantly and positively with the alteration of plasma Hcys during low-salt to high-salt intake and high-salt to high-salt with potassium supplementation (r = 0.75, P < 0.001; r = 0.74, P < 0.001, respectively). Our results indicate that Hcys may partly mediate the impact of high-salt intake and potassium supplementation on BP in SS subjects.

Obesity may promote intervertebral disc degeneration (IDD) by non-mechanical means, by influencing levels of free fatty acids which could impair cell metabolism. This study aims to establish metabolic factors in obesity-related IDD independent of mechanical loading. In clinical study, we retrospectively reviewed 128 volunteers (73 males, 55 females, aged 29–88 years) and compared their grades of disk degeneration with obesity-related factors such as body weight, BMI, and serum lipid levels. Clinically, the IDD group showed increased age, BMI and serum triglyceride. Triglyceride was a significant risk factor for IDD even after correction for BMI and age (P = 0.007). In obesity animal model, rats were fed a high-fat diet (HFD) in order to study its effects on disk metabolism and apoptosis. HFD rats had significantly higher serum levels of lipids, including triglyceride and non-esterified fatty acid, and showed significantly decreased markers of anabolism, increased catabolism and apoptosis in disk. Finally, rat nucleus pulposus (NP) cells were stimulated in vitro with a fatty acid (palmitic acid, PA) to gauge its effects on cell metabolism and apoptosis. Cell culture studies showed that NP cells exposed to PA showed increased apoptosis for activation of caspase 3, 7, 9, and PARP, which was primarily via the MAPK signal pathway, especially ERK pathway. In conclusion, hypertriglyceridemia can lead to IDD, independently of age and BMI. Hypertriglyceridemia appears to mediate disk cell apoptosis and matrix catabolism primarily via the ERK pathway.

Notodasus Fauchald, 1972 is a small genus of the polychaete family Capitellidae, including 10 described species worldwide. The genus is unusual in the Central Indo-Pacific, and there is no taxonomic record of Notodasus in this region. In this study, two new species of Notodasus are described and illustrated, namely Notodasus celebensis sp. nov. and N. chinensis sp. nov. The former species, collected from the mixed-species seagrass beds in the Indonesian island of Sulawesi, is mainly characterized by the longitudinally striated epithelium on thoracic segments and the completely separated notopodial lobes. The latter species, obtained from coastal waters off southern China, differs from its congeners with the following characters: tessellated epithelium present on anterior thorax as well as on the dorsum of chaetigers 11 and 12, notopodial lobes fused and chaetal fascicles almost touching each other on anterior abdomen, and branchial pores evident from anterior abdomen. Comparisons are made with closely related species in this paper, and a revised key is provided to all described Notodasus species. The descriptions of the two new species represent the first record of Notodasus in this region and expand the geographical distribution of the genus.

Activation of osteoclast formation and function is crucial for the development of osteolytic diseases such as osteoporosis. RANKL (receptor activator of nuclear factor-κB ligand) activates NF-κB (nuclear factor κB), MAPK (mitogen-activated protein kinase), and NFATc1 (nuclear factor of activated T-cells, cytoplasmic 1) signaling pathways to induce osteoclastogenesis. In this study, we demonstrated that SB239063, a p38-specific inhibitor, suppressed osteoclastogenesis and bone resorption via inhibiting phosphorylation of MEF2C (myocyte enhancer factor 2C) and subsequently leading to MEF2C degradation by ubiquitination. Knockdown of MEF2C impaired osteoclast formation due to decreased c-Fos expression. Furthermore, MEF2C can directly bind to the promoter region of c-Fos to initiate its transcription. Interestingly, overexpression of either MEF2C or c-Fos can partially rescue the inhibitory effect of SB239063 on osteoclastogenesis. In addition, in vivo data proved that SB239063 also played a preventive role in both LPS (lipopolysaccharide)- and OVX (ovariectomy)-induced bone loss in mice. In conclusion, our results show that SB239063 can be a potential therapy for osteolytic diseases, and a novel p38/MEF2C/c-Fos axis is essential for osteoclastogenesis.

Microbial community assembly in engineered biological systems is often simultaneously influenced by stochastic and deterministic processes, and the nexus of these two mechanisms remains to be further investigated. Here, three lab-scale activated sludge reactors were seeded with identical inoculum and operated in parallel under eight different sludge retention time (SRT) by sequentially reducing the SRT from 15 days to 1 day. Using 16S rRNA gene amplicon sequencing data, the microbial populations at the start-up (15-day SRT) and SRT-driven (≤10-day SRT) phases were observed to be noticeably different. Clustering results demonstrated ecological succession at the start-up phase with no consistent successional steps among the three reactors, suggesting that stochastic processes played an important role in the community assembly during primary succession. At the SRT-driven phase, the three reactors shared 31 core operational taxonomic units (OTUs). Putative primary acetate utilizers and secondary metabolizers were proposed based on K-means clustering, network and synchrony analysis. The shared core populations accounted for 65% of the total abundance, indicating that the microbial communities at the SRT-driven phase were shaped predominantly by deterministic processes. Sloan’s Neutral model and a null model analysis were performed to disentangle and quantify the relative influence of stochastic and deterministic processes on community assembly. The increased estimated migration rate in the neutral community model and the higher percentage of stochasticity in the null model implied that stochastic community assembly was intensified by strong deterministic factors. This was confirmed by the significantly different α- and β-diversity indices at SRTs shorter than 2 days and the observation that over half of the core OTUs were unshared or unsynchronized. Overall, this study provided quantitative insights into the nexus of stochastic and deterministic processes on microbial community assembly in a biological process.

Aiming at the diversity of Nondominated Sorting Genetic Algorithm II (NSGA-II) in screening out nondominated solutions, a crowding distance elimination (CDE) method is proposed. Firstly, the crowding distance is calculated in the same level of nondominated solutions, and the solution of minimum crowding distance is eliminated; secondly, the crowding distance of residual solutions is recalculated, and the solution of minimum crowding distance is also eliminated. Repeat the above process, and stop the cycle when the nondominated solutions reaches the set number. In order to verify the effectiveness of the algorithm, experiments are carried out with the representative test functions: ZDT1, ZDT2, and ZDT3. The comparative experiments of NSGA-II, σ-Multi-objective particle swarm optimization algorithm (MOPSO), Non dominated sorting particle swarm optimization algorithm (NSPSO), and CDE were carried out respectively. By analyzing the diversity and convergence of the four algorithms, the strategy of nondominant solutions selection based on CDE has better performance.

The present study was aimed at investigating the effects of sodium butyrate and sodium β-hydroxybutyrate on lactation and health of dairy cows fed a high-concentrate (HC) diet. Eighty mid-lactation dairy cows with an average milk yield of 33.75 ± 5.22 kg/d were randomly allocated to four groups (n = 20 per group) and were fed either a low-concentrate (LC) diet, a HC diet, the HC diet with 1% sodium butyrate (HCSB), or the HC diet with 1% sodium β-hydroxybutyrate (HCHB). The feeding trial lasted for 7 weeks, with a 2-week adaptation period and a 5-week measurement period, and the trial started from 96 ± 13 d in milk. Sodium butyrate supplementation delayed the decline in milk production and improved milk synthesis efficiency and milk fat content. Additionally, it decreased the proinflammatory cytokines and acute phase proteins (APPs) in plasma, the leucocytes in blood, the somatic cell count (SCC) in milk, and the gene expression of pattern recognition receptors (PRRs) and proinflammatory cytokines in the mammary gland, due to decreasing the contents of bacterial cell wall components (lipopolysaccharide, LPS; peptidoglycan, PGN; and lipoteichoic acid, LTA) in the rumen and plasma, compared with the HC diet. Sodium β-hydroxybutyrate supplementation also improved milk yield, milk synthesis efficiency and milk fat content and partially reduced the adverse effects caused by the HC diet, but it had no effect on decreasing bacterial cell wall components in the rumen and plasma, compared with the HC diet. Collectively, both sodium butyrate and sodium β-hydroxybutyrate mitigated the negative effects of HC diet on lactation and health of dairy cows, with sodium butyrate being more effective than sodium β-hydroxybutyrate.

High-grain diets are strongly associated with metabolic disorders in beef steers. Metabolomics can be used to explore the relationship between diet and metabolic changes, but no study has reported rumen and plasma metabolomics profiling associated with increasing dietary corn proportions in the diet of beef steers. Therefore, 12 steers paired according to similar body weights and body condition scores were randomly allocated to one of two diets: a low-corn (28.76%) diet (LCD) with a 40:60 ratio of concentrate to roughage and a high-corn (48.76%) diet (HCD) with a 60:40 ratio of concentrate to roughage. Metabolomics profiling by ultra-high-performance liquid tandem chromatography quadrupole time of flight mass spectrometry (UHPLC-QTOF/MS) showed that steers fed the HCD had increased rumen and plasma carbohydrate metabolites and amino acids, which contributed to the growth of the beef steers. However, the rumen acidity and ruminal and plasma lipopolysaccharide (LPS) concentrations significantly increased with the increase amounts of corn in the diet. In total, 717 rumen metabolites and 386 plasma metabolites were identified. By multivariate analysis, 144 rumen and 56 plasma metabolites were further identified that were significantly different between the two groups (P < 0.05 and variable influence on projection > 1). The differential metabolites in the rumen and plasma were associated with different metabolic pathways, and phenylalanine, tyrosine and tryptophan biosynthesis and phenylalanine metabolism were common key metabolic pathways for the two biofluids. In conclusion, the high-corn diet improved the growth performance of beef steers but decreased the ruminal pH and increased the LPS and harmful metabolites in the rumen and blood, which has implications for the incidence of metabolic diseases. The identified differential metabolites in both the rumen and plasma and the related metabolic pathways may contribute to the exploration of potential biomarkers for high-corn diet-based metabolic diseases.

Abstract Intensive studies on low‐temperature deposited electron transport materials have been performed to improve the efficiency of n‐i‐p type planar perovskite solar cells to extend their application on plastic and multijunction device architectures. Here, a TiO2 film with enhanced conductivity and tailored band edge is prepared by magnetron sputtering at room temperature by hydrogen doping (HTO), which accelerates the electron extraction from perovskite photoabsorber and reduces charge transfer resistance, resulting in an improved short circuit current density and fill factor. The HTO film with upward shifted Fermi level guarantees a smaller loss on VOC and facilitates the growth of high‐quality absorber with much larger grains and more uniform size, leading to devices with negligible hysteresis. In comparison with the pristine TiO2 prepared without hydrogen doping, the HTO‐based device exhibits a substantial performance enhancement leading to an efficiency of 19.30% and more stabilized photovoltaic performance maintaining 93% of its initial value after 300 min continuous illumination in the glove box. These properties permit the room‐temperature magnetron sputtered HTO film as a promising electron transport material for flexible and tandem perovskite solar cell in the future.

The inflammatory response after polymer-based drug-eluting stent (DES) placement has recently emerged as a major concern. The biologic roles of peroxisome proliferator-activated receptor-γ (PPAR-γ) activators thiazolidinedione (TZD) remain controversial in cardiovascular disease. Herein, we investigated the antiinflammatory effects of pioglitazone (PIO) on circulating peripheral blood mononuclear cells (MNCs) in patients after coronary DES implantation. Methods and Results. Twenty-eight patients with coronary artery disease and who underwent DES implantations were randomly assigned to pioglitazone (30 mg/d; PIO) or placebo (control; Con) treatment in addition to optimal standard therapy. After 12 weeks of treatment, plasma concentrations of high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and matrix metalloproteinase-9 (MMP-9) were significantly decreased in PIO group compared to the Con group (P=0.035, 0.011, 0.008, and 0.012, resp.). DES-induced mRNA expressions of IL-6, TNF-α, and MMP-9 in circulating MNC were significantly blocked by PIO (P=0.031, 0.012, and 0.007, resp.). In addition, PIO markedly inhibited DES-enhanced NF-κB function and DES-blocked PPAR-γ activity. Mechanically, DES induced PPAR-γ ubiquitination and degradation in protein level, which can be totally reversed by PIO. Conclusion. PIO treatment attenuated DES-induced PPAR loss, NF-κB activation, and proinflammation, indicating that PIO may have a novel direct protective role in modulating proinflammation in DES era.

Although precipitation extremes exert a major influence on populations and the environment, trend analysis for the entire Qinghai-Tibetan plateau (QTP) has not previously been undertaken. In this study, meteorological data from 83 weather stations was used to analyze the temporal trends and spatial distribution of 10 extreme precipitation indices over the QTP during 1975–2014. The Mann Kendall test and Sen’s slope estimator were used to determine significances and magnitudes of station trends. Spatially, stations recording increasing trends were mainly distributed in the southwestern, central and northern regions. Stations with decreasing trends were centered on the eastern and southeastern areas. Temporally, all indices had an increasing trend except for consecutive dry days (CDD) and consecutive wet days (CWD) during the study period. The contribution of extreme precipitation to total precipitation showed a significant increasing trend. These findings may be due to the complex interaction between the large-scale circulation and topography. In general, the changes of extreme precipitation indicated an enhanced tendency, with the frequency, intensity and amount of heavy precipitation increasing over time. Furthermore, altitude dependency of extreme precipitation does not exist in QTP, with topography effects on changes in precipitation extremes being more obvious in the higher elevation, flat, and hill stations.

Inversion of aerosol optical depth (AOD) over bright land surface by optical remote sensing is particularly challenging because surface reflectance dominates the satellite signal. A structure function method is suitable and can effectively solve aerosol optical depth inversion in high reflectance areas. How to select d (the pixel distance between two pixels) value is one of the key problems with the structure function method. We present a method based on geostatistics, where variogram theory is referenced, to determine the pixel distance in structure function model for aerosol optical depth inversion. This method was validated by the Moderate Resolution Imaging Spectroradiometer (MODIS) 1 km resolution level 1B data from Beijing, China. The results indicate that the relationship between variogram and d in four different directions can be fitted by exponential function, of which correlation coefficients are all above 0.9. Compared with the MODIS aerosol product (MOD 04 product), the inversion AOD has higher accuracy, with an absolute error of −0.00187 instead of −0.00854 and a relative error of 0.99% instead of 4.35%, based on AERosol RObotic NETwork (AERONET) observations. For validation, we applied this new method separately to both Beijing–Tianjin–Hebei region and MODIS 500 m resolution images for region and resolution validation. The results show that inversion AOD has higher accuracy and more efficient pixels.

A letter to the editor is presented related to infection among patients with nephrotic syndromes and systemic vasculitis treated with mycophenolate mofetil.

In order to develop small loading and small damping, a small magneto-rheological fluid (MRF) damper with built-in magnetic coils is researched, and the dynamics model of new mini-damper is established based on testing the mechanical properties of the damper. It is found that the damping landing force adjustable range will be best when the damping gap is 1.5 mm. The loading force of the mini-damper is only 1.95 N–8.25 N by adjusting the coil current from 0 A–0.8 A. The smooth damping force is the third-order function with the current by polynomial fitting of the experimental data. The result of dynamics tests shows hysteresis damping characteristics, and an improved nonlinear dynamic model is proposed by combining with the structure characteristics. The parameters of the improved dynamic model are identified by using parameter identification and regression fitting. It will provide the basis for the application of the mini-MRF damper. [ABSTRACT FROM AUTHOR]

In near-infrared spectroscopy analysis, the accuracy of instrument wavelength and breadth is crucial as it forms the foundation for transferring different instrument models. Wavelength and absorbance drift in grating-based scanning instruments result from differences in the grating, detector, and wavelength scanning system. Spectral wavelength drift can be corrected using materials such as polyethylene and polystyrene, which have absorption peaks in the near-infrared region. To reduce absorbance drift in spectra at different wavelength points, this article proposes a point-by-point linear correction method for near-infrared spectra using a small number of typical agricultural product samples. The method constructs a linear relationship model for the absorbance of each wavelength point between the main instrument and slave instruments. The study used seven S450 grating-based diffuse reflection near-infrared spectroscopy instruments, one serving as the main instrument and the remaining six as slave instruments. The point-by-point linear correction method was used to correct wheat spectra collected by the slave instruments, and a crude protein content model for wheat was established for prediction. The results showed that the method reduces spectral differences between different instruments, improves spectral consistency, and reduces prediction errors, achieving better model sharing between instruments. After correcting, the average normalized variation coefficient of wheat spectra decreased by 95.12%, from 7.78% to 0.38%, and the average standard deviation of the predicted results decreased by 78.18%, from 0.5321 to 0.1161. The correction effect of the method combined with traditional preprocessing methods was better than using pre-processing methods alone. Overall, the point-bypoint correction method based on a small number of typical agricultural product samples has a significant effect on improving the accuracy of near-infrared spectroscopy analysis. [ABSTRACT FROM AUTHOR]

Quantum resources, such as discord and entanglement, are crucial in quantum information processing. In this paper, quantum resources are studied from the aspect of quantum state superposition. We define the local superposition (LS) as the superposition between basis of single part, and nonlocal superposition (NLS) as the superposition between product basis of multiple parts. For quantum resource with nonzero LS, quantum operation must be introduced to prepare it, and for quantum resource with nonzero NLS, nonlocal quantum operation must be introduced to prepare it. We prove that LS vanishes if and only if the state is classical and NLS vanishes if and only if the state is separable. From this superposition aspect, quantum resources are categorized as superpositions existing in different parts. These results are helpful to study quantum resources from a unified frame. [ABSTRACT FROM AUTHOR]

Microorganisms are important sources of various natural products that have been commercialized for human medicine and animal healthcare. Bacitracin is an important antibacterial natural product predominantly produced by Bacillus licheniformis and Bacillus subtilis, and it is characterized by a broad antimicrobial spectrum, strong activity and low resistance, thus bacitracin is extensively applied in animal feed and veterinary medicine industries. In recent years, various strategies have been proposed to improve bacitracin production. Herein, we systematically describe the regulation of bacitracin biosynthesis in genus Bacillus and its associated mechanism, to provide a theoretical basis for bacitracin overproduction. The metabolic engineering strategies applied for bacitracin production are explored, including improving substrate utilization, using an enlarged precursor amino acid pool, increasing ATP supply and NADPH generation, and engineering transcription regulators. We also present several approaches of fermentation process optimization to facilitate the industrial large-scale production of bacitracin. Finally, the challenges and prospects associated with microbial bacitracin synthesis are discussed to facilitate the establishment of high-yield and low-cost biological factories. [ABSTRACT FROM AUTHOR]

Aiming at the shortcomings of the current Canny edge detection method in terms of noise removal, threshold setting, and edge recognition, this paper proposes a method for improving Canny edge detection by geo‐information mapping. The shortcomings of the traditional Canny edge detection method are analyzed by using the Canny optimal criterion and Tobler's First Law, which points out the direction of edge detection optimization by using the difference between edge properties and noise properties. The property characteristics and spatial distribution rules of edge points and edge lines are inspected using the geographic information mapping theory and technical methods, and edge identification criteria are defined at two levels of edge points and edge lines. Finally, the method model of improved Canny edge detection is constructed by combining guided filtering. The experimental results show that the improved edge detection method has the advantages of enriched edge details, accurate edge recognition, and strong self‐adaptive capability. This is a new attempt of geo‐information mapping theory and technical method in image edge detection, which has certain theoretical significance and strong practical guidance. [ABSTRACT FROM AUTHOR]

The features of ultrasonic bonding interface were inspected by using a high resolution transmission electron microscope. Stress of ultrasonic bonding interface was analysed by the finite elements simulation. Results show that the high stress of bonding interface was caused by ultrasonic vibration, which increased the dislocation density inside the metal crystalline lattice which provides the fast diffusion channels, and provided driving force for atom inter-diffusion. 'Short-circuit diffusion' during ultrasonic bonding is more prominent than crystal diffusion. For the given ultrasonic bonding parameters, depth of atom diffusion at Au/Al interface of ultrasonic bonding was about 100-300 nm in several ten milliseconds, which forms the bonding strength of 0.65 N, and it is an inter-metallic compound of AuAl2. These will be helpful for further analysis. [ABSTRACT FROM AUTHOR]

Accurate lithostratigraphy framework of the Central Qilian belt is key to understand subduction-collision of the Proto-Tethyan Ocean in the NE Tibetan Plateau. The Dongchagou and the Moshigou formations share similar detrital zircon age populations with youngest age peak at ca. 1.15--1.21 Ga, which is consistent with the granitoid gneiss of the Liujiatai Formation. In combination with rock assemblages, metamorphic facies, and associated oldest granitoid plutons, we revised Precambrian lithostratigraphy of the Central Qilian belt as the >1.2 Ga Huangyuan Group and the ca. 1200--930 Ma Huangzhong Group in this study. The former is the same as the Liujiatai Formation, whereas the later includes the Dongchagou and Moshigou formations. Siliciclastic turbidites of the Qingshipo Formation gradually pass upward into carbonates of the Huashishan Group, we therefore ascribe them to the Huashishan Group. This group unconformably overlies the Precambrian basement of the Central Qilian belt and consists of continental slope to coastal plain deposits with a bi-direction of the S- and N-ward paleocurrent. These sediments contain abundant metamorphic, magmatic, and minor ophiolitic detritus with main age populations of ca. 910 Ma, ca. 487 Ma, and ca. 447 Ma detrital zircons, probably derived from the Central and South Qilian belts. The basement of the Huangzhong Group experienced 440--430 Ma continent-continent collision-related metamorphism and deformation. These results and regional geology demonstrate that the Huashishan Group was formed by arc-continent collision deposition during culmination of the Proto-Tethyan Ocean closure in the Qilian orogenic belt, NE Tibetan Plateau. [ABSTRACT FROM AUTHOR]

Polo-like kinase 1 (Plk1) plays essential roles during multiple stages of mitosis by phosphorylating a number of substrates. Here, we report that the atypical protein kinase Rio2 is a novel substrate of Plk1 and can be phosphorylated by Plk1 at Ser-335, Ser-380, and Ser-548. Overexpression of Rio2 causes a prolonged mitotic exit whereas knockdown of Rio2 accelerates mitotic progression, suggesting that Rio2 is required for the proper mitotic progression. Overexpression of phospho-mimicking mutant Rio2 S3D but not the nonphosphorylatable mutant Rio2 S3A displays a profile similar to that of wild-type Rio2. These results indicate that the phosphorylation status of Rio2 correlates with its function in mitosis. Furthermore, time-lapse imaging data show that overexpression of Rio2 but not Rio2 S3A results in a slowed metaphase-anaphase transition. Collectively, these findings strongly indicate that the Plk1-mediated phosphorylation of Rio2 regulates metaphase-anaphase transition during mitotic progression. [ABSTRACT FROM AUTHOR]

Pretreatment of waste-activated sludge (WAS) is an effective way to destabilize sludge floc structure and release organic matter for improving sludge digestion efficiency. Nonetheless, information on the impact of WAS pretreatment on digestion sludge microbiomes, as well as mechanistic insights into how sludge pretreatment improves digestion performance, remains elusive. In this study, a genome-centric metagenomic approach was employed to investigate the digestion sludge microbiome in four sludge digesters with different types of feeding sludge: WAS pretreated with 0.25 mol/liter alkaline/acid (APAD), WAS pretreated with 0.8 mol/liter alkaline/acid (HSAPAD), thermally pretreated WAS (thermal-AD), and fresh WAS (control-AD). We retrieved 254 metagenome-assembled genomes (MAGs) to identify the key functional populations involved in the methanogenic digestion process. These MAGs span 28 phyla, including 69 yet-to-be-cultivated lineages, and 30 novel lineages were characterized with metabolic potential associated with hydrolysis and fermentation. Interestingly, functional populations involving carbohydrate digestion were enriched in APAD and HS-APAD, while lineages related to protein and lipid fermentation were enriched in thermal-AD, corroborating the idea that different substrates are released from alkaline/acid and thermal pretreatments. Among the major functional populations (i.e., fermenters, syntrophic acetogens, and methanogens), significant correlations between genome sizes and abundance of the fermenters were observed, particularly in APAD and HS-APAD, which had improved digestion performance. [ABSTRACT FROM AUTHOR]

The NF-κB family member RelB is an important transcription factor that is capable of regulating diverse immune and inflammatory responses. However, its role in the regulation of Foxp3+ regulatory T cells (Tregs) in vivo is poorly defined. In this study, we demonstrated that germline deletion of Relb resulted in systemic autoimmunity, which is associated with significant accumulation of Foxp3+ Tregs in lymphoid and nonlymphoid organs. Foxp3+ Tregs from RelB-deficient mice were functional and capable of suppressing T effector cells in vitro and in vivo, but Foxp3- T effector cells from RelB-deficient mice showed features of hyperactivation and spontaneously produced high levels of IL-2. Surprisingly, mice with conditional deletion of Relb in T cells (Cd4CreRelbf/f mice) or specifically in Foxp3+ Tregs (Foxp3CreRelbf/f mice) did not show signs of autoimmunity and had similar frequencies of Foxp3+ Tregs in the periphery as wild-type C57BL/6 controls. Both strains of conditional knockout mice also had a normal conventional T cell compartment. However, reconstituting Rag-1-/-Relb-/- hosts with wild-type C57BL/6 bone marrow cells led to hyperactivation of T effector cells, as well as marked expansion of Foxp3+ T cells. These data suggest that the autoimmune phenotype in germline RelB-deficient mice is most likely caused by T cell-extrinsic mechanisms, and further studies are warranted to uncover such mechanisms. [ABSTRACT FROM AUTHOR]

This study proposes a surface-enhanced Raman scattering (SERS) chip integrated with a liquid core waveguide. Due to the total reflection, the chip suppresses the leaky waveguide mode and enables a long propagation distance for SERS signal collection. A 10 nM rhodamine 6G Raman signal was obtained using a QE65000 portable microspectrometer at a low excitation power intensity of 14.5 W/cm². The proposed SERS chip was also used to detect the SERS spectrum of a silkworm DNA solution, the structural information of which was clearly shown. In addition, due to the lower excitation power density, damage to the structure of the biomolecule was avoided. [ABSTRACT FROM AUTHOR]