951. [Mechanistic study of nimesulide on enhancing gammadeltaT cell-mediated killing of gastric cancer cells].
- Author
-
Liu JQ, Chen FX, Gong XJ, Li HZ, Cai K, Zhang BF, Zhang S, and Zhang J
- Subjects
- Cell Line, Tumor, Cells, Cultured, Granzymes immunology, Humans, Interferon-gamma immunology, Perforin immunology, T-Lymphocyte Subsets enzymology, Tumor Necrosis Factor-alpha immunology, Cytotoxicity, Immunologic drug effects, Perforin drug effects, Stomach Neoplasms immunology, Sulfonamides pharmacology, T-Lymphocyte Subsets immunology
- Abstract
Aim: To explore the effect of nimesulide on human gammadeltaT cell function., Methods: gammadeltaT cells were cultured routinely, collected on the 9th day, and then induced with nimesulide at indicated concentrations (0.25 micromol/L, 0.5 micromol/L, 1 micromol/L, 2 micromol/L, 4 micromol/L, respectively). Twenty-four hours after induction, the supernatants were collected to detect IFN-gamma, TNF-alpha and IL-12, whereas the cells were assayed for perforin, granzyme B and NKG2D by flow cytometry (FCM) and for killing of gastric cancer cells by LDH., Results: Nimesulide (1 micromol/L) caused gammadeltaT cells to express more of perforin and granzyme B (62.8% and 72.7%, respectively) than the control group (51.4% and 60.9%, respectively) (P<0.05). Likewise, nimesulide (1 micromol/L) enabled gammadeltaT cells to secret more of IFN-gamma and TNF-alpha (262.3 ng/L and 177.5 ng/L, respectively) than the control group (196.1 ng/L and 158.5 ng/L, respectively) (P<0.05). Nimesulide did not affect IL-12 secreting capability of gammadeltaT cells as compared with the control group (P>0.05). Nimesulide-stimulated gammadeltaT cells killed more of SGC-7901 and BCG-823 gastric cancer cells (73% and 70%, respectively) than the control group(54% and 53%, respectively) (P<0.05)., Conclusion: Nimesulide made gammadeltaT cells to express more perforin and granzyme B and to secret more IFN-gamma and TNF-alpha into the supernatant, leading to higher killing rate of SGC-7901 and BCG-823 gastric cancer cells. The above data provides experimental basis on the clinical use of nimesulide to prevent and treat digestive tract tumors.
- Published
- 2010