910 results on '"Dan Bi"'
Search Results
902. Antiviral Effects of Black Raspberry (Rubus coreanus) Seed and Its Gallic Acid against Influenza Virus Infection.
- Author
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Lee JH, Oh M, Seok JH, Kim S, Lee DB, Bae G, Bae HI, Bae SY, Hong YM, Kwon SO, Lee DH, Song CS, Mun JY, Chung MS, and Kim KH
- Subjects
- Animals, Antiviral Agents administration & dosage, Antiviral Agents isolation & purification, Chromatography, Liquid, Disease Models, Animal, Gallic Acid administration & dosage, Gallic Acid isolation & purification, Influenza A virus physiology, Influenza B virus physiology, Mice, Inbred BALB C, Orthomyxoviridae Infections drug therapy, Survival Analysis, Tandem Mass Spectrometry, Viral Plaque Assay, Antiviral Agents pharmacology, Gallic Acid pharmacology, Influenza A virus drug effects, Influenza B virus drug effects, Rubus chemistry, Seeds chemistry, Virus Replication drug effects
- Abstract
Influenza is a serious public health concern worldwide, as it causes significant morbidity and mortality. The emergence of drug-resistant viral strains requires new approaches for the treatment of influenza. In this study, Rubus coreanus seed (RCS) that is left over from the production of wine or juice was found to show antiviral activities against influenza type A and B viruses. Using the time-of-addition plaque assay, viral replication was almost completely abolished by simultaneous treatment with the RCS fraction of less than a 1-kDa molecular weight (RCSF1). One of the polyphenols derived from RCSF1, gallic acid (GA), identified by liquid chromatography-tandem mass spectrometry, showed inhibitory effects against both influenza type A and B viruses, albeit at relatively high concentrations. RCSF1 was bound to hemagglutinin protein, inhibited hemagglutination significantly and disrupted viral particles, whereas GA was found to only disrupt the viral particles by using transmission electron microscopy. In BALB/c mice infected with influenza virus, oral administration of RCSF1 significantly improved the survival rate and reduced the viral titers in the lungs. Our results demonstrate that RCSF1 and GA show potent and broad antiviral activity against influenza A and B type viruses and are promising sources of agents that target virus particles.
- Published
- 2016
- Full Text
- View/download PDF
903. Dual growth factor-immobilized microspheres for tissue reinnervation: in vitro and preliminary in vivo studies.
- Author
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Kim TH, Oh SH, An DB, Lee JY, and Lee JH
- Subjects
- Animals, Cells, Cultured, Drug Carriers chemistry, Drug Combinations, Drug Evaluation, Preclinical, Fibroblast Growth Factor 2 pharmacokinetics, Materials Testing, Mice, Mice, Hairless, Muscle, Skeletal drug effects, Muscle, Skeletal innervation, Nerve Growth Factor pharmacokinetics, Neurogenesis drug effects, Pilot Projects, Rats, Drug Carriers chemical synthesis, Fibroblast Growth Factor 2 administration & dosage, Microspheres, Nerve Growth Factor administration & dosage, Nerve Regeneration drug effects
- Abstract
Growth factors (GFs) (basic fibroblast growth factor (bFGF) and/or nerve growth factor (NGF))-immobilized polycaprolactone (PCL)/Pluronic F127 microspheres were prepared using an isolated particulate-melting method and the sequential binding of heparin and GFs onto the microspheres. The GFs immobilized on the microspheres were released in a sustained manner over 28 days, regardless of GF type. From the in vitro culture of muscle-derived stem cells, it was observed that the NGF-immobilized microspheres induced more neurogenic differentiation than the bFGF-immobilized microspheres, as evidenced by a quantitative real-time polymerase chain reaction using specific neurogenic markers (Nestin, GFAP, β-tubulin, and MAP2) and Western blot (Nestin and β-tubulin) analyses. The dual bFGF/NGF-immobilized microspheres showed better neurogenic differentiation than the microspheres immobilized with single bFGF or NGF. From the preliminary animal study, the dual bFGF/NGF-immobilized microsphere group also showed effective nerve regeneration, as evaluated by immunocytochemistry using a marker - β-tubulin. The dual bFGF/NGF-immobilized PCL/Pluronic F127 microspheres may be a promising candidate for nerve regeneration in certain target tissues (i.e. muscles) leading to sufficient reinnervation.
- Published
- 2015
- Full Text
- View/download PDF
904. A comparative study of low-fluence 1,064 nm Q-Switched Nd:YAG laser with or without chemical peeling using Jessner's solution in melasma patients.
- Author
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Lee DB, Suh HS, and Choi YS
- Subjects
- Asian People, Combined Modality Therapy, Double-Blind Method, Drug Combinations, Humans, Patient Satisfaction, Chemexfoliation methods, Ethanol therapeutic use, Lactic Acid therapeutic use, Lasers, Solid-State therapeutic use, Low-Level Light Therapy methods, Melanosis therapy, Resorcinols therapeutic use, Salicylates therapeutic use
- Abstract
Background: Although low-fluence 1,064-nm Q-switched Nd:YAG laser (QSNYL) is widely used for the treatment of melasma, multiple treatments are necessary for clinical improvement. Superficial chemical peeling using Jessner's solution has been used for treatment of melasma conventionally., Objectives: To evaluate the additional therapeutic effect and adverse effects of Jessner's peel when combined with 1,064 nm QSNYL for melasma patients in a double-blind, placebo-controlled design., Methods: Total of 52 patients were included. Patients who received 10 sessions of 1,064 nm QSNYL plus chemical peeling with placebo (Group A) in a two-week intervals and those who received 10 sessions of 1,064 nm QSNYL plus chemical peeling with Jessner's solution (Group B) in a 2-week intervals were analyzed. Responses were evaluated using the Melasma Area and Severity Index (MASI) score, physician's global assessment (PGA) and subjective self-assessment., Results: At 8 weeks, the mean MASI score decreased from 8.68 ± 4.06 to 8.60 ± 3.88 in Group A and from 8.98 ± 3.72 to 7.13 ± 2.57 in Group B, showing a significant difference (p < 0.001). But at 20 weeks, there was no significant difference on reduction of MASI, self-assessment, and PGA between the two groups. No serious adverse effects were reported with the additional Jessner's peeling., Conclusion: This study suggests Jessner's peel is a safe and effective method in the early course of treatment for melasma, when combined with low-fluence 1,064-nm QSNYL.
- Published
- 2014
- Full Text
- View/download PDF
905. Human temporal lobe epilepsy analyses by tissue proteomics.
- Author
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Mériaux C, Franck J, Park DB, Quanico J, Kim YH, Chung CK, Park YM, Steinbusch H, Salzet M, and Fournier I
- Subjects
- Adult, Dentate Gyrus metabolism, Dentate Gyrus surgery, Epilepsy, Temporal Lobe surgery, Female, Hippocampus surgery, Humans, Intracellular Signaling Peptides and Proteins, Male, Middle Aged, Proteins metabolism, Sex Characteristics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem Mass Spectrometry, Young Adult, Epilepsy, Temporal Lobe metabolism, Hippocampus metabolism, Proteomics methods
- Abstract
Although there are many types of epilepsy, temporal lobe epilepsy (TLE) is probably in humans the most common and most often studied. TLE represents 40% of the total epilepsy form of the disease and is difficult to treat. Despite a wealth of descriptive data obtained from the disease history of patients, the EEG recording, imaging techniques, and histological studies, the epileptogenic process remains poorly understood. However, it is unlikely that a single factor or a single mechanism can cause many changes associated with this neuropathological phenomenon. MALDI mass spectrometry imaging (MSI) coupled to protein identification, because of its ability to study a wide range of molecules, appears to be suitable for the preparation of molecular profiles in TLE. Seven neuropeptides have been have been identified in Dental gyrus regions of the hippocampus in relation with TLE pathology. Shot-gun studies taking into account gender influence have been performed. Tissue microextraction from control (10) toward 10 TLE patients have been analyzed after trypsin digestion followed by separation on nanoLC coupled to LTQ orbitrap. From the shot-gun analyses, results confirmed the presence of specific neuropeptides precursors and receptors in TLE patients as well as proteins involved in axons regeneration including neurotrophins, ECM proteins, cell surface proteins, membrane proteins, G-proteins, cytoskeleton proteins and tumor suppressors. Among the tumor suppressors identified, the Leucine-rich glioma inactivated 1 (LGI1) protein was found. LGI1 gene recently been demonstrated being implicated in heritability of TLE. We have also demonstrate the presence a complete profile of tumor suppressors in TLE patients, 7 have been identified. Refining this analysis taken into account the gender influence in both control and in TLE reflected the presence of specific proteins between male and female and thus mechanisms in pathology development could be completely different., (© 2014 Wiley Periodicals, Inc.)
- Published
- 2014
- Full Text
- View/download PDF
906. A case of intravascular myopericytoma.
- Author
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Park HJ, Lee DR, Park MY, Choi YS, and Lee DB
- Subjects
- Aged, Female, Humans, Soft Tissue Neoplasms pathology, Hemangiopericytoma pathology, Orbital Neoplasms pathology, Vascular Neoplasms pathology
- Published
- 2010
- Full Text
- View/download PDF
907. Double staining immunohistochemistry.
- Author
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Chen X, Cho DB, and Yang PC
- Abstract
This article summarized immunohistochemistry methods generally used in research laboratories and clinic including direct immune staining, indirect immune staining, enzyme method, fluorescence method, APC method and PAP method.
- Published
- 2010
- Full Text
- View/download PDF
908. Exception discovery: a novel method for the identification of differentially expressed proteins.
- Author
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Shim JE, Kim DB, Lee WS, Cho SY, and Yu E
- Subjects
- Carcinoma, Hepatocellular metabolism, Female, Humans, Liver metabolism, Liver Neoplasms metabolism, Male, Middle Aged, Peptide Mapping, Proteome analysis, Proteome metabolism, Reproducibility of Results, Electrophoresis, Gel, Two-Dimensional methods, Proteins analysis, Proteins classification, Proteins metabolism, Proteomics methods, Statistics, Nonparametric
- Abstract
The identification of differentially expressed proteins (DEPs) observed under specific conditions is one of the key issues in proteomics research. There are currently several ways to detect the changes of a specific protein's expression level in two-dimensional electrophoresis (2-DE) gel images such as statistical analysis and graphical visualization. However, it is quite difficult to handle the information of an individual protein manually by these methods due to the large distortions of patterns in 2-DE images. This paper proposes a method of analyzing DEPs for a specific disease. In order to automatically extract meaningful DEPs in a set of 2-DE gel images, we have designed an exception function that is suitable to measure the anomalous change of the expression level of an individual protein. We present the comparison results of the proposed method versus a Wilcoxon paired t -test that is one of the widely used statistical analysis methods. Several experiments are performed to address not only the effectiveness of the exception function but also the fact that these two methods can compensate each other practically.
- Published
- 2010
- Full Text
- View/download PDF
909. 1-Methyl-3-n-tetra-decyl-imidazolium bromide monohydrate.
- Author
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Chen Y, Song W, Xu J, Yang XR, and Tian DB
- Abstract
In the title ionic liquid salt hydrate, C(18)H(35)N(2) (+)·Br(-)·H(2)O, the side chain in the cation has an extended conformation. The crystal structure is stabilized primarily by O-H⋯Br hydrogen bonds. C-H⋯O and C-H⋯Br inter-actions are also present.
- Published
- 2009
- Full Text
- View/download PDF
910. 3,3'-Dimethyl-1,1'-ethyl-ene-diimidazolium dibromide.
- Author
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Chen Y, Song W, Xu J, Cui RB, and Tian DB
- Abstract
The title compound, C(10)H(16)Br(2)N(4), was synthesized by the reaction of 1-methyl-imidazole and 1,2-dibromo-ethane in toluene. The complete dication is generated by a crystallographic inversion centre situated at the mid-point of the ethane C-C bond. In the crystal structure, weak inter-molecular C-H⋯Br inter-actions link the mol-ecules into chains along the b axis and an intramolecular C-H⋯Br close contact is also present.
- Published
- 2009
- Full Text
- View/download PDF
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