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Objective: To observe the inhibitive effect of cyclooxygenase-2 (COX-2) selective inhibitor, [N-[2-(cyclohexyloxy)-4-nitrophenyl]-methane sulfonamide] (NS398, one of nonsteroidal anti-inflammatory drugs), on human ovarian cancer cell lines CAOV3 and OVCAR3 during the proliferative process in vitro., Methods: Streptovidin peroxidase conjugated (SP) immunohistochemical assay was performed to examine the expression of COX-2 protein in human ovarian epithelial serous cancer cell lines CAOV3 and OVCAR3 respectively. The proliferative inhibition process of the two cell lines by NS398 was observed with methyl thiazolyl tetrazolium (MTT) rapid photocolorimetric assay. Cell morphologic changes were observed under the inverted phase contrast microscope and electron microscope. DNA ladder assay and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay were employed for the cell apoptosis., Results: COX-2 protein was expressed in both of the cell lines. The inhibitory rate of proliferation exerted by NS398 increased with the density and the time of NS398 respectively. In contrast to the control, the absorbance of the experiment (NS398 200 micro mol/L for 72 h) decreased obviously (P < 0.05). With regard to the cell morphology, the "vacuole" presented in the cytoplasm and apoptosis body was able to be observed, and the microvilli on the surface of the cell disappeared. There were characteristic ladder bands after CAOV3 and OVCAR3 were treated by NS398 (100 micro mol/L) for 72 h. The brown-yellow granules located in the nucleus of most CAOV3 cells, which indicated apoptosis., Conclusions: Above findings suggest that COX-2 may provide an effective chemotherapeutic target for ovarian cancer, and selective COX-2 inhibitors can be used as chemotherapeutic agents for ovarian cancer.

To study the preventive effect of itraconazole against fungal infections following chemotherapy in tumor patients, we conducted a double-blind randomized clinical trial in 114 tumor patients receiving chemotherapy, who were divided into itraconazole treatment group (n=52) and control group (n=62) and the incidences of fungal infections after chemotherapy were recorded. In comparison with the control group, itraconazole treatment group exhibited a significant decrease in fungal infection episodes (12.8% vs 3.8%), illustrating the efficacy of itraconazole treatment as a preventive measure against fungal infections secondary to chemotherapy.

Objective: To study the mechanism involved in the control of glioma cell proliferation with transfection of connexin (Cx) 43 gene., Methods: C6 rat glioma and TJ905 human glioblastoma cell lines without Cx43 gene expression were transfected with Cx43cDNA mediated by lipofectamine. Northern blot, in situ hybridization and immunohistochemical technology were used to detect the expression of Cx43mRNA and its protein with MTT assay and silver colloid stain for the detection of cell proliferation, TUNEL method for determination of cell apoptosis, scrape loading dye transfer (SLDT) for GJIC, Western blot and immunohistochemical technology for bFGF, PDGF, EGFR, IGF-I and IGFBP3 expression., Results: Cx 43 gene transfected glioma cells showed decreased proliferation, restored GJIC and decreased bFGF, PDGF, IGFBP3, except EGFR expression and cell apoptosis which showed no change., Conclusion: The mechanism of Cx 43 gene inhibiting gliomas cell proliferation is the restoration of GJIC and decreased autocrine growth factors.

The effects of four surfactants and microemulsion were studied in the detection on trace manganses in food by flame atomic absorption spectrometry. The results showed that the absorption increased when one of two of surfactants and microemulsion were used. The microemulsion are the most effective. The absorption increased by 58%. A method of determination on trace manganese by means of atomic absorption spectrometry sensitized with microemulsion was established. At the same time, we also made a recovery experiment for trace manganese in food, The relative standard deviation is 2.90%-5.62%, and recovery is 97.0%-98.2%.