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AIM: To observe whether silicone oil (SO) tamponade could decrease macular perfusion after retinal detachment repair. METHODS: A prospective observational case-control study. Patients diagnosed with primary macular off rhegmatogenous retinal detachment undergoing successful retinal repair surgery with vitrectomy were strictly selected. Optical coherence tomography angiography findings were compared between SO and air tamponade groups. Two postoperative visiting points were set (1 and 3mo). RESULTS: Totally 29 patients (29 eyes) were enrolled. Twenty cases had SO tamponade while 9 cases were with air tamponade. At the first visiting point, superficial parafoveal vessel density (PFSVD) significantly decreased in the SO group (P=0.0403), especially in the superior quadrant or superior-hemi area (P=0.0089, 0.0426, respectively). Parafoveal deep vessel density (PFDVD) had no difference between the two groups. At the second visiting point, all quadrants of PFSVD reduced significantly in the SO group (P=0.0256, 0.0001, 0.0031,

Objective: Ultrasound (US) plays an important role in the diagnosis and management of breast diseases; however, effective breast US screening is lacking in rural and remote areas. To alleviate this issue, we prospectively evaluated the clinical availability of 5G-based telerobotic US technology for breast examinations in rural and remote areas. Methods: Between September 2020 and March 2021, 63 patients underwent conventional and telerobotic US examinations in a rural island (Scenario A), while 20 patients underwent telerobotic US examination in a mobile car located in a remote county (Scenario B) in May 2021. The safety, duration, US image quality, consistency, and acceptability of the 5G-based telerobotic US were assessed. Results: In Scenario A, the average duration of the telerobotic US procedure was longer than that of conventional US (10.3 ± 3.3 min vs. 7.6 ± 3.0 min, p = 0.017), but their average imaging scores were similar (4.86 vs. 4.90, p = 0.159). Two cases of gynecomastia, one of lactation mastitis, and one of postoperative breast effusion were diagnosed and 32 nodules were detected using the two US methods. There was good interobserver agreement between the US features and BI-RADS categories of the identical nodules (ICC = 0.795–1.000). In Scenario B, breast nodules were detected in 65% of the patients using telerobotic US. Its average duration was 10.1 ± 2.3 min, and the average imaging score was 4.85. Overall, 90.4% of the patients were willing to choose telerobotic US in the future, and tele-sonologists were satisfied with 85.5% of the examinations. Conclusion: The 5G-based telerobotic US system is feasible for providing effective breast examinations in rural and remote areas.

This paper addresses the two-dimensional loading open vehicle routing problem with time window (2L-OVRPTW). We propose a learning whale optimization algorithm (LWOA) to minimize the total distance; an improved skyline filling algorithm (ISFA) is designed to solve the two-dimensional loading problem. In LWOA, the whale optimization algorithm is used to search the solution space and get the high-quality solution. Then, by learning and accumulating the block structure and customer location information in the high-quality solution individuals, a three-dimensional matrix is designed to guide the updating of the population. Finally, according to the problem characteristics, the local search method based on fleet and vehicle is designed and performed on the high-quality solution region. IFSA is used to optimize the optimal individual. The computational results show that the proposed algorithm can effectively solve 2L-OVRPTW. [ABSTRACT FROM AUTHOR]

Abstract Adult patients with relapsed or refractory T‐cell acute lymphoblastic leukemia (R/R‐T‐ALL) have extremely poor prognosis, representing an urgent unmet medical need. Finding an optimal salvage regimen to bridge transplantation is a priority. The CAG (cytarabine, aclarubicin, and G‐CSF) regimen was initially used by one group in China, showing unexpectedly promising results in 11 R/R‐T‐ALL patients. Here, we report the multicenter results of 41 patients who received the CAG regimen as salvage therapy. After one cycle of the CAG regimen, complete remission and partial remission were achieved in 33 (80.5%) and two (4.9%) patients, respectively. Failure to respond was observed in six patients (14.6%). Early T‐cell precursor (ETP) (n = 26) and non‐ETP (n = 15) patients had a similar CR rate (80.8% vs 80.0%, P = .95). Among 41 patients, allo‐HSCT was successfully performed in 27 (66%) patients (22 in CR and 5 in non‐CR). With a median follow‐up time of 12 months, the estimated 2‐year overall survival and event‐free survival were 68.8% (95% CI, 47.3%‐83.0%) and 56.5% (95% CI, 37.1%‐71.9%), respectively. The CAG regimen was well‐tolerated, and no early death occurred. Our multicenter results show that the CAG regimen is highly effective and safe, representing a novel choice for adult patients with R/R‐T‐ALL and providing a better bridge to transplantation.

Abstract Previous studies have shown that forkhead box P4 antisense RNA 1 (FOXP4‐AS1) is dysregulated in tumor tissues and can serve as a prognostic indicator for multiple cancers. However, the clinical significance of FOXP4‐AS1 in pancreatic ductal adenocarcinoma (PDAC) remains unclear. The goal of this study is to recognize the possible clinical significance of long noncoding RNA FOXP4‐AS1 in patients with early stage PDAC. A total of 112 patients from The Cancer Genome Atlas (TCGA) PDAC cohort, receiving RNA sequencing, were involved in the study. Survival analysis, functional mechanism, and potential small molecule drugs of target therapy of FOXP4‐AS1 were performed in this study. Survival analysis in TCGA PDAC cohort suggested that patients with high FOXP4‐AS1 expression had significantly augmented possibility of death than in PDAC patients with lower FOXP4‐AS1 expression (adjusted P = .008; adjusted HR = 2.143, 95% CI = 1.221‐3.760). In this study, a genome‐wide RNA sequencing dataset was used to identify 927 genes co‐expressing with FOXP4‐AS1 in PDAC tumor tissues. A total of 676 differentially expressed genes were identified between different FOXP4‐AS1 expression groups. Functional enrichment analysis of these genes and gene set enrichment analysis for PDAC genome‐wide RNA sequencing dataset was done. We have found that FOXP4‐AS1 may function in PDAC by participating in biological processes and pathways including oxidative phosphorylation, tricarboxylic acid cycle, classical tumor‐related pathways such as NF‐kappaB as well as Janus kinase/signal transducers in addition to activators of transcription, cell proliferation, and adhesion. In addition, we also screened two potential targeted therapeutic small molecule drugs (dimenhydrinate and metanephrine) for FOXP4‐AS1 in PDAC. In conclusion, our present study demonstrated that higher expression of FOXP4‐AS1 in PDAC tumor tissues were related with an inferior medical outcome. Through multiple genome‐wide approaches, we identified the potential molecular mechanisms of FOXP4‐AS1 in PDAC and two targeted therapeutic drugs for it.

The use of exogenous functional microorganisms to regulate biogenic amine (BA) content is a common approach in fermentation systems. Here, to better understand the microbial traits of succession trajectories in resource-based and biotic interference systems, the BA-related primary and secondary succession were tracked during industrial semidry Chinese rice wine (CRW) fermentation. Dominant abundance and BA-associated microbial functionality based on phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) indicated that Citrobacter, Acinetobacter, Lactobacillus, Exiguobacterium, Bacillus, Pseudomonas, and Enterobacter spp. prominently contributed to the decarboxylase gene family in CRW. The expression levels of tyrosine decarboxylase (tyrDC), ornithine decarboxylase (odc), and agmatine deiminase (aguA) genes were assessed by quantitative PCR (qPCR). The transcription levels of these genes did not correlate with the BA formation rate during postfermentation, indicating that acidification and carbon source depletion upregulated the expression and microbes launch the dormancy strategy to respond to unfavorable conditions. Furthermore, microbial interference with CRW fermentation by Lactobacillus plantarum (ACBC271) and Staphylococcus xylosus (CGMCC1.8382) coinoculated at a ratio of 1:2 exhibited the best synergetic control of BA content. Spearman correlations revealed that Lactobacillus and Staphylococcus exhibited influence on BA-associated microbiota (|ρ| > 0), Exiguobacterium and Pseudomonas were strongly suppressed by Lactobacillus (ρ = -0.867 and ρ = -0.782, respectively; P < 0.05), and Staphylococcus showed the strongest inhibitory effect toward Lactobacillus (ρ = -0.115) and Citrobacter (ρ = -0.188) in the coinoculated 1:2 group. The high inhibitory effect of exogenous added strains on specific bacteria presented evidence for the obtained BA-associated contributors. Overall, this work provides important insight into the microbial traits that rely on resource usage and functional microbiota within food microbial ecology. [ABSTRACT FROM AUTHOR]

Spontaneous spinal epidural haematoma (SSEH) is a rare clinical condition with unknown aetiology. Prompt diagnosis and treatment are paramount because of the risk of permanent neurological deficits without appropriate intervention. We described a case of a 48-year-old man presenting with complete quadriplegia and hypoesthesia. Magnetic resonance imaging revealed cervical cord compression due to a haematoma posterior to the spinal cord. Surgical decompression and evacuation of the haematoma was performed within 12 hours after admission to the authors' hospital. Both the patient's motor and sensory functions recovered soon after the operation. Early surgical decompression for SSEH with neurologic impairment is therefore recommended for the recovery of this patient and also serves as a relevant reference for orthopaedic clinics. Foundation number: CXZZ20140414170821148.

Prenatal alcohol exposure disrupts the development of normal fetal respiratory function, but whether it perturbs respiratory rhythmical discharge activity is unclear. Furthermore, it is unknown whether the 5-hydroxytryptamine 2A receptor (5-HT 2A R) is involved in the effects of prenatal alcohol exposure. In the present study, pregnant female rats received drinking water containing alcohol at concentrations of 0%, 1%, 2%, 4%, 8% or 10% (v/v) throughout the gestation period. Slices of the medulla from 2-day-old neonatal rats were obtained to record respiratory rhythmical discharge activity. 5-HT 2A R protein and mRNA levels in the pre-Bötzinger complex of the respiratory center were measured by western blot analysis and quantitative RT-PCR, respectively. Compared with the 0% alcohol group, respiratory rhythmical discharge activity in medullary slices in the 4%, 8% and 10% alcohol groups was decreased, and the reduction was greatest in the 8% alcohol group. Respiratory rhythmical discharge activity in the 10% alcohol group was irregular. Thus, 8% was the most effective alcohol concentration at attenuating respiratory rhythmical discharge activity. These findings suggest that prenatal alcohol exposure attenuates respiratory rhythmical discharge activity in neonatal rats by downregulating 5-HT 2A R protein and mRNA levels.

A facile co-precipitation method has been successfully developed to prepare Ternary transition metal CrNbO4 (CNO-CP) with high crystallinity. FESEM images show the particles of CNO-CP distribute homogeneously without agglomeration. The electrochemical test results show that the CNO-CP electrode maintains a capacity of 210 mA h g-1 after 50 cycles at 16 mA g-1 higher than 89 mA h g-1 of CrNbO4 (CNO-SS) prepared by solid state method. The rate capabilities of the CNO-CP at 32, 80 and 160 mA g-1 are 232.4, 176.8 and 137.1 mA h g-1, respectively, which are also superior to CNO-SS. [ABSTRACT FROM AUTHOR]

Using a radio-frequency tunnel diode oscillator technique, we measured the temperature dependence of the in-plane London penetration depth Δλah(T) in Fe1+y(Te1-xSex) single crystals, down to temperatures as low as 50 mK. A significant number of samples, with nominal Se concentrations x = 0.36, 0.40, 0.43, and 0.45, respectively, were studied and in many cases we found that Δλah(T) shows an upturn below 0.7 K, indicative of a paramagnetic-type contribution. After subtracting the magnetic background, the low-temperature behavior of penetration depth is best described by a power law with exponent n ≈ 2 and with no systematic dependence on the Se concentration. Most importantly, in the limit of T → 0, in some samples we observed a narrow region of linear temperature dependence of penetration depth, suggestive of nodes in the superconducting gap of Fe1+y(Te1-xSex). [ABSTRACT FROM AUTHOR]

• Heat stress increased proline, soluble sugar, electrolyte leakage, and ascorbate peroxidase. • Proline concentration may act as an indicator in response to heat stress in petunia. • PhP5CR could be taken as a biomarker of proline pathways in response to heat stress. • Among PhHsfs, PhHsf19 and PhHsf21 are key players in regulating heat tolerance. High temperature is a critical constraint factor limiting plant growth and productivity of crops throughout the world. Petunia (Petunia hybrida) is a well-known ornamental plant which is widely grown in gardens. However, petunia does not tolerate high temperatures well and seedling production and landscape uses are seriously inhibited in the summer. In this study, a total of ten physiological indexes were selected to analyze the physiological responses of petunia 'W115' under heat stress. Partial least squares-discriminant analysis showed that the physiological responses of petunia 'W115' to heat stress could be classified into three critical time points (0.5 h, 3 h, and 72 h after stress), and that proline concentration was the most important core physiological index to reflect the heat-stress response of petunia seedlings. In order to elucidate the molecular regulatory mechanism of heat-stress response in petunia 'W115', the expression patterns of eight proline metabolism pathway genes and of 12 heat shock transcription factors (Hsfs) were studied by real-time quantitative PCR under heat stress. Expression analysis indicated that the expression trend of the proline synthetase gene (PhP5CR) was most closely correlated with the change in proline concentration, and the expression levels of most Hsfs were markedly up-regulated, especially PhHsf19 and PhHsf21 , which were highly expressed during the entire heat-stress period, suggesting that these genes could play important roles in response to heat stress in petunia 'W115'. We hope this research could deepen our understanding of the heat-stress response mechanism of petunia 'W115' seedlings and identify candidate genes for molecular breeding for heat tolerance in the future. [ABSTRACT FROM AUTHOR]

Realizing precise control over multiquanta emission is crucial for quantum information processing, especially when integrated with advanced techniques of manipulating quantum states. Here, by spinning the resonator to induce the Sagnac effect, we can obtain nonreciprocal photon-phonon and photon-magnon super-Rabi oscillations under conditions of optically driving resonance transitions. Opening dissipative channels for such super-Rabi oscillations enables the realization of directional bundle emissions of entangled photon-phonon pairs and photon-magnon pairs by transferring the pure multiquanta state to a bundled multiquanta outside of the system. This nonreciprocal emission is a flexible switch that can be controlled with precision, and simultaneous emissions of different entangled pairs (such as photon-phonon or photon-magnon pairs) can even emerge but in opposite directions by driving the resonator from different directions. This ability to flexibly manipulate the system allows us to achieve directional entangled multiquanta emitters, and has also potential applications for building hybrid quantum networks and on-chip quantum communications.

Cells can sense and process various signals. Noise is inevitable in the cell signaling system. In a bacterial community, the mutual conversion between normal cells and persistent cells forms a bidirectional phenotype switching cascade, in which either one can be used as an upstream signal and the other as a downstream signal. In order to quantitatively describe the relationship between noise and signal amplification of each phenotype, the gain-fluctuation relationship is obtained by using the linear noise approximation of the master equation. Through the simulation of these theoretical formulas, it is found that the bidirectional phenotype switching can directly generate interconversion noise which is usually very small and almost negligible. In particular, the bidirectional phenotype switching can provide a global fluctuating environment, which will not only affect the values of noise and covariance, but also generate additional intrinsic noise. The additional intrinsic noise in each phenotype is the main part of the total noise and can be transmitted to the other phenotype. The transmitted noise is also a powerful supplement to the total noise. Therefore, the indirect impact of bidirectional phenotype switching is far greater than its direct impact, which may be one of the reasons why chronic infections caused by persistent cells are refractory to treat.

We demonstrate symmetry protected multiphoton bundle emission in the cavity QED system under the ultrastrong coupling regime. Our proposal only enables the super-Rabi oscillations with periodic generation of even correlated photons in the cavity, which is realized by combining the laser driven flip of qubit and the symmetry conserved transitions induced by Rabi interaction with parity symmetry. Combined with dissipation, only 2n-photon bundle emissions are allowed, due to the almost perfect suppression of bundle emissions with odd correlated photons. Meanwhile, the corresponding purities are significantly enhanced by the parity symmetry. This work extends multiphoton bundle emission to the ultrastrong coupling regime, and offers the prospect of exploring symmetry-protected multiphoton physics.

Like genes and proteins, cells can use biochemical networks to sense and process information. The differentiation of the cell state in colonic crypts forms a typical unidirectional phenotypic transitional cascade, in which stem cells differentiate into the transit-amplifying cells (TACs), and TACs continue to differentiate into fully differentiated cells. In order to quantitatively describe the relationship between the noise of each compartment and the amplification of signals, the gain factor is introduced, and the gain-fluctuation relation is obtained by using the linear noise approximation of the master equation. Through the simulation of these theoretical formulas, the characters of noise propagation and amplification are studied. It is found that the transmitted noise is an important part of the total noise in each downstream cell. Therefore, a small number of downstream cells can only cause its small inherent noise, but the total noise may be very large due to the transmitted noise. The influence of the transmitted noise may be the indirect cause of colon cancer. In addition, the total noise of the downstream cells always has a minimum value. As long as a reasonable value of the gain factor is selected, the number of cells in colonic crypts will be controlled within the normal range. This may be a good method to intervene the uncontrollable growth of tumor cells and effectively control the deterioration of colon cancer.

We demonstrate theoretically the bundle emission of n strongly correlated phonons in an acoustic cavity QED system. The mechanism relies on Stokes resonances that generate super-Rabi oscillations between states with a large difference in their number of excitations, which, combined with dissipation, transfer coherently pure n-phonon states outside of the cavity. This process works with close to perfect purity over a wide range of parameters and is tunable optically with well-resolved operation conditions. This broadens the realm of quantum phononics, with potential applications for on-chip quantum information processing, quantum metrology, and engineering of new types of quantum devices, such as optically heralded n-phonon guns.

Objective: To observe the effect of acupoint catgut embedding on the expression of nucleotide-binding oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome, interleukin (IL)-1β and IL-18 in the uterine tissue of primary dysmenorrhea (PD) rats, so as to explore its underlying mechanisms in improving PD., Methods: Forty female SD rats were randomly divided into control, model, acupoint catgut embedding and medication groups ( n ＝10 in each group). The PD model was established by subcutaneous injection of Estradiol Benzoate (0.5 mg/rat on the 1 st and 10 th day, and 0.2 mg/rat from 2 nd to 9 th day) and Oxytocin (2 U/rat, i．p.). The catgut embedding was applied to bilateral "Sanyinjiao" (SP6) and "Guanyuan" (CV4) on the 1 st and 5 th day after modeling. Rats of the medication group were treated by intragastric perfusion of Fenbid (0.8 mL/rat, 125 mg/100 mL) once daily for 10 days. The body writhing times in 30 min were recorded. The histopathological changes of the uterine were observed by H．E. staining. Western blot was used to detect the expression of NLRP 3, caspase-1, IL-1β and IL-18 in uterine tissues., Results: The body writhing times were notably more in the model group than in the control group ( P <0.01), and obviously fewer in both medication and catgut embedding groups than in the model group ( P <0.05, P <0.01). After modeling, the rats' endometrium was extensively exfoliated and got swelling, the histopathological score and the expression levels of NLRP3, caspase-1, IL-1β and IL-18 proteins in the uterus tissue were evidently increased in the model group relevant to the control group ( P <0.01). Following the treatment, the degree of endometrial exfoliation and edema of the uterus tissue was lightened, the pathological score was significantly reduced ( P <0.01), and the expression levels of caspase-1, IL-1β and IL-18 protein in uterus tissue were markedly decreased in both acupoint catgut embedding and medication groups ( P <0.01, P <0.05). The NLRP3 protein expression was significantly decreased in the acupoint catgut embedding group compared with that in the model group ( P <0.01). The therapeutic effect of acupoint catgut embedding was significantly superior to that of medication in reducing writhing times and down-regulating expression of NLPR3 protein ( P <0.01). No significant differences were found between catgut embedding and medication in histopathological score, and expression levels of caspase-1, IL-1β and IL-18 proteins ( P >0.05)., Conclusion: The acupoint catgut embedding can significantly alleviate the symptoms and pathological damage in PD rats, which may be related to its effect in inhibiting the activation of NLRP3 inflammasome in the uterine tissue.

Smoke water and distillation liquid were used to treat the seeds of Trichosathes kirilowii and to study the effects of smoke water and distillation liquid on the seed germination and seedling growth of T. kirilowii. The results showed that germination rate, germination index and germination vigor of T. kirilowii all were significantly improved with the treatment of SW and DL treatment. The activity of α-amylase were significantly increased with the treatment of SW and DL at 1:2,000. SW and DL treatment showed no significant effects on the activity of SOD. The activity of POD were markedly enhanced under the treatment of SW (1:000) and DL (1:2,000). CAT activity were increased with the treatment of SW and DL at 1:2,000 while were inhibited by SW and DL at 1:500. Seedling height and root length were increased with the treatment of SW and DL (1:1,000, 1:2,000). SW and DL treaments improved the content of chlorophyll, and moreover with the concentration of SW and DL, the stimulatory were also increased. This work demonstrated that smoke water and diatillation liquid at 1:2,000 could stimulate the seed germination and seedling growth of T. kirilowii, and it provided the references for the study of seed germination technology.