837 results on '"Huang, Lu-Qi"'
Search Results
802. [Effects of methyl jasmonat on accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy root].
- Author
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Wang XY, Cui GH, Huang LQ, and Qiu DY
- Subjects
- Abietanes, Culture Techniques, Plant Growth Regulators pharmacology, Plant Roots growth & development, Plant Roots metabolism, Plants, Medicinal drug effects, Plants, Medicinal growth & development, Plants, Medicinal metabolism, Salvia miltiorrhiza growth & development, Salvia miltiorrhiza metabolism, Acetates pharmacology, Cyclopentanes pharmacology, Oxylipins pharmacology, Phenanthrenes metabolism, Plant Roots drug effects, Salvia miltiorrhiza drug effects
- Abstract
Objective: To study the effects of methyl jasmonate (MJ) on the accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy roots., Method: After 18 day's suspension culture of S. miltiorrhiza hairy roots induced by Agrobacterium rhizogenes ATCC15834, the chemical elicitor--methyl jasmonat was added into 6-7V suspension cultures and at the same time, tanshinones contents (including cryptotanshinone and tanshinone II(A)) on the day 2, 6 and 9, after dealing with MJ, was quantified by HPLC., Result: After dealing with MJ on the day 2, 6 and 9, the concentration of cryptotanshinone reached to 0.039, 0.204, 0.571 mg x g(-1) respectively,and tanshinone II(A) reached 0.251, 0.601 and 1.563 mg x g(-1) respectively. After 9 day's treatment by MJ, the maximum increase of cryptotanshinone and tanshinone II(A) were 23.8 fold and 6.2 fold higher than that of the control respectively., Conclusion: MJ could stimulate the accumulation of tanshinones in S. miltiorrhiza and have released them into the culture medium.
- Published
- 2007
803. [Secondary metabolites accumulating and geoherbs formation under enviromental stress].
- Author
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Huang LQ and Guo LP
- Subjects
- Carbon metabolism, Cold Temperature, Ecosystem, Hot Temperature, Plants, Medicinal growth & development, Water, Drugs, Chinese Herbal metabolism, Environment, Plants, Medicinal metabolism
- Abstract
This paper analyzed how habitat affected the formation of geoherbs after summarizing the influences of environmental stress on plants growth, especially on theirs secondary metabolites accumulating, and introducing 4 kinds hypothesis about environmental stress affects plants. It was then pointed out that environmental stress may have advantage on the formation of geoherbs. The stress effect hypothesis on forming geoherbs was brought forward, and the ways and methods on study the geoherbs under environmental stress was discussed.
- Published
- 2007
804. [Primary study on shapes of fruits and germination characters of seeds of Radix et Rhizoma Rhei].
- Author
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Xiao SP, Chen M, Huang LQ, and Gao F
- Subjects
- Benzyl Compounds, Gibberellins pharmacology, Kinetin pharmacology, Plants, Medicinal anatomy & histology, Plants, Medicinal physiology, Purines, Rheum anatomy & histology, Species Specificity, Temperature, Fruit anatomy & histology, Germination drug effects, Plant Growth Regulators pharmacology, Rheum physiology, Seeds physiology
- Abstract
Objective: To explore the difference of the shapes of fruits and germination characters of seeds of Rheum palmatum, R. tanguticum and R. officinale., Method: The seeds of three Rheum species including wild and cultivation one were collected from different regions. Character of the fruits appearance were observed. The purification, weights per thousand seeds, content of moisture, seed vigor and different germination rates of Radix et Rhizoma Rhei were measured. The germination rates of the seeds of R. palmatum were determined under different temperature and hormone treatment ., Result: It was markedly variant in shapes of the fruits, rates of germination and all quality characters of the three category of Radix et Rhizoma Rhei. As reported, the size of the fruit and seed and weights per thousand seeds of R. officinale was the biggest. The seed vigor and germination rate of R. palmatum caltivated in Ming county of Gangsu were 95.7% and 94% respectively. The rate of seed vigor was closely correlated with the rate of germination for other species. Results indicated that temperature had some effects on the germination, and there were obvious difference in the active effect of gibberellin, kinetin and 6-benzyl aminopurine on the germination of the seed. Our experimental results showed that the kinetin concentration of 50 x 10(-6) g x L(-1) had the most active effect, and its rates of germination increase 18 percent than the control one., Conclusion: Significant differences exist in the shapes of fruits, rates of germination of the seeds of Rheum, which are affected by the factors of temperatures and hormones. The result of this study provides scientific evidences for identify, growth and cultivation of Rheum.
- Published
- 2007
805. [Discussion on method of synthetic assessment for threaten and priority conservation of medicinal plants].
- Author
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Huang LQ, Tang SH, Cui GH, and Xiao PG
- Subjects
- China, Conservation of Natural Resources economics, Ethnobotany economics, Ethnobotany methods, Plants, Medicinal chemistry, Conservation of Natural Resources methods, Plants, Medicinal growth & development
- Abstract
According to the resource of medicinal plant's present situation and characteristic, we took the primary discussion on the method of synthetic assessment to the threat and priority conservation of medicinal plants, and constituted the corresponding system of synthetic assessment, regarding four aspects: coefficience of value, coefficience of distribution, coefficience of biology, coefficience of present situation, etc., and fifteen minor terms as standards.
- Published
- 2006
806. [Application of multiplex allele-specific PCR for authentication of Panax ginseng and P. quinquefolius].
- Author
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Cui GH, Tang XJ, and Huang LQ
- Subjects
- Alleles, DNA Primers, DNA, Plant genetics, Genetic Markers, Panax classification, Plant Roots genetics, Plants, Medicinal classification, Reproducibility of Results, Species Specificity, Panax genetics, Plants, Medicinal genetics, Polymerase Chain Reaction methods, Polymorphism, Single Nucleotide
- Abstract
Objective: Searching and identifying SNP in Panax species and using multiplex allele-specific PCR (MAS-PCR) to authenticate P. ginseng and P. quenquefolium., Method: Based on genbank database of Panax species, using DNAMAN to align the sequences, identify SNP of P. ginseng and P. quenquefolium. Design allele-specific primers for P. ginseng and P. quenquefolium, optimize the PCR reaction system including the usage amount of Taq, dNTP, primer, etc. Optimized system was performed with the total DNA of 20 different sources of P. ginseng and P. quenquefolium., Result: When the annealing temperature was 66 'C, the template DNA of P. ginseng could be amplified 249 bp band whereas P. quenquefolium amplified 1 049 bp band., Conclusion: The MAS-PCR have the advantages of highly specific, good reproducibility and could be identify P. ginseng and P. quenquefolium in the same PCR tube. It was a potential method to use in the molecular identification of other meteria medica.
- Published
- 2006
807. [A new molecular method to authenticate radix trichosanthis as well as its adulterants and substitutes].
- Author
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Cui GH, Huang LQ, and Wang M
- Subjects
- DNA Primers, DNA, Plant genetics, DNA, Ribosomal Spacer, Drug Contamination prevention & control, Random Amplified Polymorphic DNA Technique methods, Reproducibility of Results, Trichosanthes chemistry, Trichosanthes classification, Trichosanthin isolation & purification, Polymorphism, Genetic, Trichosanthes genetics, Trichosanthin genetics
- Abstract
Objective: To explore a new molecular method to authenticate Radix Trichosanthis., Method: Three 20 mer primers based on the ITS sequence was designed. The PCR reaction system was optimized and applied to nineteen different sources of Radix Trichosanthis and nine adulterants and substitutes., Result: Polymorphic map of Radix Trichosanthis and its adulterants was obtained from primer TKS1-64. 560 bp and 960 bp bands were authentic markers for Radix Trichosanthis., Conclusion: Primer TKS1-64F possesses the advantages of good stability and reproducibility. This new method is named as anchored primer amplification polymorphism DNA(APAPD). It was a potential method to used in molecular identification of other meteria medica.
- Published
- 2006
808. [Genetic diversity of Eucommia ulmoides by RAPD analysis].
- Author
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Wang AQ, Huang LQ, Shao AJ, Cui GH, Chen M, and Tong CH
- Subjects
- China, Conservation of Natural Resources, DNA, Plant genetics, Eucommiaceae classification, Genetics, Population, Phylogeny, Random Amplified Polymorphic DNA Technique, Eucommiaceae genetics, Plants, Medicinal genetics, Polymorphism, Genetic
- Abstract
Objective: To determine the genetic diversity of Eucommia ulmoides., Method: 260 samples of 20 populations were analyzed through radom amplified polymorphic DHA (RAPD)., Result: Total polymorphic loci percentage was 96.36 and the average was 38.92. 110 bands were produced with 10 random primers and 106 were polymorphic. Nei's gene diversity (H) was 0.246 1, Shannon's Information index(I) was 0.386 8, Gst was 0.424 4, indicating that 42.44% of the genetic variation was distributed among populations and 57.65% within populations., Conclusion: The genetic variation was relatively high in E. ulmoides, so the genetic diversity conservation principle should mainly focus on protection of the populations.
- Published
- 2006
809. [Effects of Arbuscular Mycorrhizae on growth and essential oil of Atractylodes lancea].
- Author
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Guo LP, Wang HG, Huang LQ, Jiang YX, Zhu YG, Kong WD, Chen BD, Chen ML, Lin SF, and Fang ZG
- Subjects
- Nitrogen analysis, Phosphorus analysis, Potassium analysis, Soil analysis, Soil Microbiology, Atractylodes growth & development, Atractylodes metabolism, Atractylodes microbiology, Mycorrhizae physiology, Oils, Volatile metabolism, Plants, Medicinal growth & development, Plants, Medicinal metabolism, Plants, Medicinal microbiology
- Abstract
Objective: To study the effects of Arbuscular Mycorrhizae on cultivated Atractylodes lancea., Method: Pot experiment of A. lancea, with (code as AM) or without (code as CK) Glomus mosseae (GM) was conducted 5 times respectively, then the biomass, essential oil, and soil nutrition, soil organism, soil microbial were detected after A. lancea were harvested., Result: (1) Mycorrhizal dependency of A. lancea was 245%, and height of individuals, numbers of leaves, leaf area, biomass of A. lancea were all higher in AM than in CK (P < 0.05). (2) GC-MS analysis with cluster analysis and principal components analysis showed that there were no differences in essential oil of A. lancea between AM and CK. (T3) Total N, available N, available P and available K in AM soil were all lower than in CK soil. (4) GC-MS analysis showed organic matters changed differenly in AM soil and CK soil, components 5,6 in AM soil were higher than that in CK soil, but component 9, 10, 11 were lower in AM soil than that in CK soil. (5) Biolog detect showed AWCD of AM soil microbe were higher than that of CK soil throughout the incubation, and AWCD of the former was 0.66, and the later was 0.46 after 192 h incubation. and t-test showed, Shannon seven indices and McIntosh'seven indices were same both at 72 h and 192 h, and diversity indices of Shannon and McIntosh were also same at 72 h, but AM soil microbe were higher than CK soil microbe at 168 h (P < 0.05)., Conclusion: AM could promote nutrition uptake, improve the function diversity and activity of microbe in rhizosphere of A. lancea, influence the composition of the organic matter, that lead the growth of A. lancea, but not to the quality.
- Published
- 2006
810. [Studies on in vitro culture of adventitious root in Salvia miltiorrhiza].
- Author
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Chen W, Guo XH, Gao WY, Chen HX, Huang LQ, and Xiao PG
- Subjects
- Abietanes, Benzaldehydes metabolism, Catechols metabolism, Culture Media, Hydrogen-Ion Concentration, Phenanthrenes metabolism, Plant Growth Regulators pharmacology, Plant Roots growth & development, Plant Roots metabolism, Plants, Medicinal metabolism, Salvia miltiorrhiza metabolism, Sucrose, Tissue Culture Techniques, Plants, Medicinal growth & development, Salvia miltiorrhiza growth & development
- Abstract
Objective: To study the culture of adventitious root of Salvia miltiorrhiza in vitro systemically., Method: Effects of sucrose concentrations, medium pH, inoculum size and plant growth regulators on adventitious root growth and secondary metabolites production in S. miltiorrhiza were investigated., Result: With the increase of initial sucrose concentration, adventitious root growth rates increased and tanshinone II A content decreased, while content of protocatechuic aldehyde showed a broken line change and its highest production was obtained under 30 g x L(-1) sucrose in the medium. As for the effect of medium pH, medium pH of 6.5, 5.5 (or 6.0) and 5.8 was favorable for adventitious root growth, tanshinone II A and protocatechuic aldehyde synthesis respectively. Furthermore, adventitious root growth, rate was greatly increased when inoculum size was 2.5%. MS medium added with 0.5 mg x L(-1) KT was much favorable for tanshinone II A and protocatechuic aldehyde accumulation., Conclusion: Parameters including sucrose concentrations, medium pH, inoculum size and plant growth regulators have distinct effects on the in vitro culture of adventitious root growth and secondary metabolites synthesis of S. miltiorrhiza.
- Published
- 2006
811. [Study on genetic diversity of natural and cultivated Cistanche tubulosa].
- Author
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Cui GH, Chen M, Huang LQ, and Liu MT
- Subjects
- Conservation of Natural Resources, DNA, Plant genetics, Ecosystem, Phylogeny, Random Amplified Polymorphic DNA Technique, Cistanche genetics, Plants, Medicinal genetics, Polymorphism, Genetic
- Abstract
Objective: To determine the genetic diversity of natural and cultivated Cistanche tubulosa., Method: 123 individuals of six populations of C. tubulosa, including four natural populations and two cultivated ones, were analyzed by random amplified polymorphic DNA (RAPD) markers to determine the genetic variations among the populations., Result: A total of 87 loci (including 24 polymorphic loci) were amplified using 10 random primers. The average percentage of polymorphic loci (PPL) was 27.59 in the natural populations. The PPL between natural populations are 19.54 to 25.29. Of the four natural populations, Andi' er population had highest PPL (25.29). The two cultivated populations had low PPL (13.79 and 11.49). Cluster analysis using UPGMA revealed that populations of natural and cultivated were separated into two groups, the four natural populations clustered as one group and the two cultivated populations clustered as another group, indicating that the natural and cultivated populations had obvious differentiation., Conclusion: In view of the low genetic diversity of the cultivated C. tubulosa, we strongly suggested that the natural populations should be conserved in particular.
- Published
- 2006
812. [Experimental studies on hepatotoxicity of rats induced by Fructus Gardeniae].
- Author
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Yang HJ, Fu MH, Wu ZL, Liang RX, Huang LQ, Fang J, Li G, and Cao Y
- Subjects
- Alanine Transaminase blood, Animals, Aspartate Aminotransferases blood, Bilirubin blood, Drugs, Chinese Herbal isolation & purification, Female, Fruit chemistry, Iridoids isolation & purification, Male, Organ Size drug effects, Plants, Medicinal chemistry, Pyrans isolation & purification, Random Allocation, Rats, Rats, Sprague-Dawley, Drugs, Chinese Herbal toxicity, Gardenia chemistry, Iridoids toxicity, Liver pathology, Pyrans toxicity
- Abstract
Objective: To study the hepatotoxicity effects in rats with different extract of Fructus Gardeniae., Method: Observe the change of appearance, behavior and weight of rats through oral gavage daily for 3 d. Weigh the liver and calculate the liver index. Detect the ALT, AST and TBIL. Observe the liver tissue by optical microscope., Result: The weight and index of liver were increased by 3.08 g x kg(-1) aqueous extract, 1.62 g x kg(-1) alcoholic extract and 0.28 g x kg(-1) geniposide, compared to those of the blank group (P < 0.005, P < 0.001) and the activities of ALT, AST and the content of TBIL were also increased, compared to those of the blank group (P < 0.05, P < 0.001). The liver cells were obviously swell, necrotic and changed with inflammatory infiltrate., Conclusion: Aqueous extract, alcoholic extract and geniposide displayed hepatotoxicity, and the geniposide which was the main substance of the Fructus Gardeniae might be mainly responsible for the hepatotoxicity.
- Published
- 2006
813. [High specific PCR identification of Bungarus multicinctus and its adulterants].
- Author
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Feng CQ, Tang XJ, Huang LQ, Qian ZZ, Zhang J, and Cui GH
- Subjects
- Animals, Base Sequence, Bungarus classification, DNA Primers, Drug Contamination, Molecular Sequence Data, Polymerase Chain Reaction methods, Sequence Analysis, DNA, Snakes classification, Snakes genetics, Species Specificity, Bungarus genetics, Cytochromes b genetics, DNA genetics, Materia Medica
- Abstract
Objective: To develop a convenient and effective method for the identification of Bungarus multicinctus., Method: Based on the sequence of Cyt b gene fragment of B. multicinctus and its adulterants, a pair of highly specific primer (HJL- and HJH-) were designed for distinguishing B. ulticinctus from other species of snake. To establish specific PCR reaction condition, the primers were employed to amplify the DNA templates extracted from B. multicinctus and 6 other species of snake, under different annealing temperature. Using this method, B. multicinctus was identified from 18 samples bought from many drugstores., Result: A 230 bp DNA fragment was amplified from B. multicinctus in PCR with annealed temperature at 67 degrees C, whereas no DNA fragment was amplified from other snake samples under the same reaction condition, B. multicinctus could be clearly distinguished from others by PCR reaction with the highly specific primers. In the present study, 18 sample, bought from different drugstores, were also identified by the highly specific PCR with the primers. The results indicated that 14 samples were B. multicinctus and the other 4 were adulterant, which was consistent with the conclusion of authentication based on morphological., Conclusion: The primers designed in the present study were highly specific for B. multicinctus.
- Published
- 2006
814. [Prevention of soil deterioration during cultivation of medicinal plants].
- Author
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Guo LP, Huang LQ, Jiang YX, and Lv DM
- Subjects
- Hydrogen-Ion Concentration, Mycorrhizae growth & development, Soil Microbiology, Environmental Pollution prevention & control, Plants, Medicinal growth & development, Soil analysis, Soil parasitology
- Abstract
This paper summarized the aspects of the soil deterioration due to continuous growth of medicinal plants, such as nutrition insufficient, pH variation, harmful salt accumulating, harmful microbe and allelopathic substance increasing, soil physics and chemistry properties variation. And the ways to prevent and rehabilitate the deteriorated soil was indicated, which included anti-adversity species selecting, scientific management such as whorl cropping, nutrient elements supplement, usage of physical methods, nutrient liquid cultivating and VAM inoculating etc.
- Published
- 2006
815. [RAPD analysis of Scutellaria baicalensis from different germplasms].
- Author
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Shao AJ, Li X, Huang LQ, Lin SF, and Chen J
- Subjects
- Breeding, Cluster Analysis, DNA Primers genetics, Ecosystem, Phylogeny, Plant Leaves anatomy & histology, Plant Leaves genetics, Plants, Medicinal anatomy & histology, Random Amplified Polymorphic DNA Technique methods, Scutellaria baicalensis anatomy & histology, DNA, Plant genetics, Genetic Variation, Plants, Medicinal genetics, Scutellaria baicalensis genetics
- Abstract
Objective: To provide reference for breeding Scutellaria baicalensis from different germplasms,and to explore their genetic diversities and different strains., Method: The RAPD method was applied to the study on S. baicalensis from different germplasms by use of 14 random primers about 10 bp, and SAPD-analysis of 34 S. baicalensis from different germplasms was carried out by using the method of withingroups-linkage in SPSS 10.0., Results: 14 among 115 primers were selected to amplify about 165 segments of DNA. Among them, 132 segments of DNA, 80.0% of the total, can represent genetic of diversities of S. baicalensis. According to RAPD analysis, 34 germplasms of S. baicalensis were classified into A, B, C and D categories., Conclusion: S. baicalensis from different germplasms shows abundant genetic diversities. Germplasms from Shandong like Mengyin 3, Menyin 2 and Pingyi have close distance (0.315) in genetic background, which can be chosen for breeding of cultivated. Though genetic characters are similar in the morphology, the geological distribution of S. baicalensis and its morphology have not certain correlation. The complex genetic background of S. baicalensis indicate that the work of the selective breeding and management for breeding of S. baicalensis have to be strengthened.
- Published
- 2006
816. [Study on the stigma/pollen vigor and self-compatibility of Platycodon grandiflorum].
- Author
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Wei JH, Huang LQ, Chen SL, Cheng HZ, Yang CM, and Chu QL
- Subjects
- Plants, Medicinal growth & development, Platycodon growth & development, Reproduction physiology, Flowers physiology, Plants, Medicinal physiology, Platycodon physiology, Pollen physiology
- Abstract
Objective: To provide the basal data for the breeding and cultivation of Platycodon grandiflorum., Method: The field investigation and pollination by bagging were carried out. TTC(2,3,5-triphenyl tetrazolium chloride) solution was used to test the pollen vigor., Result: The stigma life-span of P. grandiflorum was about 9 days, however the optimal time for pollination is 4-6 days after the petals opening, with the stigma was splitting lightly or significantly. When the petals opened, the anther began scattering pollen, and finished in the same day. The pollen vigor was about 81.4% at the beginning, and decreases to 27.6% three days later. The pollen vigor still remains 64.4% three days later, when the flower was kept in the desiccant. The natural fructification rate of self-flower-pollination was 4.8%. The fructification rate and compatible index was about 62.7% and 54.6, respectively, when the self-plant-pollination performed by hand. They decreased to 12.8% and 6.5 when the pollination was implemented during the flowering period., Conclusion: Low fructification percentage of self-flower-pollination attributes to the difference of maturing period of pistil and stamen, as well as the short pollen life-span of P. grandiflorum. The compatibility of self-plant-pollination is high during flowering period when pollination performed by hand. The life-span of the pollen can be prolonged significantly when keeping in the dry environment.
- Published
- 2006
817. [Effects and mechanism of total saponins of Psammosilene tunicoids against rheumatoid arthritis].
- Author
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Wang XY, Zhang Y, Xu JY, Qiu DW, and Huang LQ
- Subjects
- Animals, Arthritis, Rheumatoid pathology, Foot pathology, Interleukin-1 metabolism, Male, Plant Roots chemistry, Plants, Medicinal chemistry, Rats, Rats, Wistar, Saponins isolation & purification, Tumor Necrosis Factor-alpha metabolism, Arthritis, Rheumatoid metabolism, Caryophyllaceae chemistry, Saponins pharmacology
- Abstract
Objective: To observe the antiarthritic effects and the possible mechanism of total saponins of Psammruosilene tunicoids (TSPT) against rheumatoid arthritis (RA)., Method: After establishing AA rat model, the TSPT'S antiarthritic effects and mechanism against RA were studied through observing the changes of ankle swelling, arthritis index and levels of IL-1beta and TNF-alpha after medication., Result: TSPT could effectively inhibits articular swelling, decrease arthritis index and regulate down the content of IL-1beta and TNF-alpha in the inflammatory tissue soak of AA rats., Conclusion: TSPT has good antiarthritic effects and the possible mechanism may be related to its down-regulation of IL-1beta and TNF-alpha.
- Published
- 2006
818. [Determination of epicatechin in Rhizoma Fagopyri dibotoryis by HPLC-ECD].
- Author
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Xin MT, Yang B, Li H, and Huang LQ
- Subjects
- Chromatography, High Pressure Liquid methods, Electrochemistry, Reproducibility of Results, Rhizome chemistry, Catechin analysis, Fagopyrum chemistry, Plants, Medicinal chemistry
- Abstract
Objective: To establish a quantitative method of determination of epicatechin in Rhizoma fagopyri dibotoryis by HPLC-electrochemical detection., Method: The sample was separated on a column of Diamonsil C18 (4.6 mm x 250 mm, 5 microm) which was eluted with methanol-0.1 mol x L(-1) phosphate buffer (1:3, pH 2.5), the flow rate was 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the applied potential was + 600 mV., Result: The linear range of epicatechin was 0.004 875-1.56 microg, r = 0.999 9. The average recovery was 100.7% with RSD 1.9% (n = 5). The content of epicatechin was different in nine samples purchased., Conclusion: The method is good reproducible and can be used to determine epicatechin in Rhizoma fagopyri dibotoryis.
- Published
- 2006
819. [The status and changes of soil nutrients in rhizosphere of cultivated Atractylodes lancea].
- Author
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Guo LP, Huang LQ, Shao AJ, Lv DM, and Wu ZG
- Subjects
- China, Ecosystem, Hydrogen-Ion Concentration, Nitrogen analysis, Organic Chemicals, Phosphorus analysis, Potassium analysis, Rhizome growth & development, Atractylodes growth & development, Plants, Medicinal growth & development, Soil analysis
- Abstract
Objective: To find the status and changes of the soil nutrients in rhizosphere of Atractylodes lancea., Method: Total nitrogen (total N), available K, available P, organic matter (ORG), available nitrogen and pH in rhizosphere soil of the wild growing A. lancea in 3 sites, MS, LT and MFS, and the cultivated ones with different ages in LT were detected., Result: The contents of total nitrogen (total N), available K, available P, organic matter (ORG), available nitrogen and pH value in rhizosphere soil were significant different between MS, LT and MFS (P < 0.01). The results of the 6 detected parameters in MS were the lowest, in MFS were the highest and in LT were in the middle. The total N, ORG and available N in the cultivated A. lancea were lower than that in the wild ones (P < 0.01) and available P and pH value in the cultivated A. lancea were higher than that in wild ones (P < 0.01) and there was no difference in available K between the wild and cultivated ones in LT (P > 0.05); 3 available P in rhizosphere soil of the two years old A. lancea were higher than of the one year old A. lancea (P < 0.01) and there were no difference of total N, ORG, available N, available K and pH value in rhizosphere soil of A. lancea between one year and two years plant (P > 0.05)., Conclusion: It is indicated that the growth of A. lancea in Mt. Mao is faced nutrient stress.
- Published
- 2005
820. [Development of the study on germplasm resources of medicinal plants: construction of core collection].
- Author
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Huang LQ, Lv DM, Yang B, Shao AJ, Chen M, and Wei JH
- Subjects
- Chromatography, High Pressure Liquid, Culture Techniques, Ecosystem, Sequence Analysis, DNA, Conservation of Natural Resources, Plants, Medicinal chemistry, Plants, Medicinal genetics, Plants, Medicinal growth & development, Polymorphism, Genetic
- Abstract
According to the successful experience of studying on crops, this paper introduced the conception of the core collection of medicinal plants, and analyzed the characteristic and the way to construct it. Studies on the core collection would fulfill the management and utilize the germplasm resources conveniently and provide a new idea and a method to study on the germplasm resources of medicinal plants. It is necessary to study on the core collection for the development of the germplasm resources of medicinal plants.
- Published
- 2005
821. [Application of "3S" technologies in continuous usage of traditional Chinese medicine resources].
- Author
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Guo LP, Huang LQ, and Jiang YX
- Subjects
- Ecosystem, Geography, Medicine, Chinese Traditional, Pest Control, Plant Diseases, Satellite Communications, Soil, Conservation of Natural Resources, Drugs, Chinese Herbal, Geographic Information Systems, Plants, Medicinal
- Abstract
This paper introduced development of 3S technologies, i.e., remote sensing (RS), geographic information system (GIS), and global positioning system(GPS), and drew the conclusion that 3S technologies can be used widely in resources dynasty survey, ecology study, and geoherbs study and classification about traditional Chinese medicine resources.
- Published
- 2005
822. [Discussion on criterions of endanger and protection levels of traditional Chinese medicine herbs].
- Author
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Cui GH and Huang LQ
- Subjects
- Pharmacognosy standards, Conservation of Natural Resources, Ecosystem, Plants, Medicinal
- Abstract
To efficiently protect the endangered traditional Chinese medicine herbs is essential for the sustainable development of traditional Chinese medicine. On the bases of present species endanger and protection levels, problems in the traditional Chinese medicine herbs are analyzed. The endangered levels of traditional Chinese medicine herbs should refer to the standard of IUCN, and the protection levels should adopt qualitative and quantitative analysis based on the characteristic of traditional Chinese medicine herbs. Some qualitative and quantitative factors are discussed, some useful information was also provided for the establishment of protection levels of traditional Chinese medicine herbs.
- Published
- 2005
823. [The determination of echinacoside and acteoside in herbs of Cistanche tubulosa].
- Author
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Chen M, Xiao SP, Cui GH, Zhang SJ, Wu ZG, Huang LQ, and Liu MT
- Subjects
- Breeding, Chromatography, High Pressure Liquid methods, Cistanche anatomy & histology, Cistanche physiology, Host-Parasite Interactions, Plants, Medicinal anatomy & histology, Plants, Medicinal physiology, Tamaricaceae classification, Tamaricaceae parasitology, Cistanche chemistry, Glucosides analysis, Glycosides analysis, Phenols analysis, Plants, Medicinal chemistry
- Abstract
Objective: To analyse the echinacoside and acteoside content of Cistanche tubulosa, collected from different hosts and different size of the cultivated, which is compared to the wilding by RP-HPLC method., Method: An Agilent Eclipse XDB-C18(4.6 mm x 250 mm, 5 microm) column was used and a mixture of methanol-acetonitrile-1% acetic acid (15:10:75) was used as the mobile phase at a flow rate of 0.6 mL x min(-1). The column temperature was 30 degrees C and the UV detection wavelength was 334 nm., Result: The calibration curves of echinacoside and acteoside were in good linearity over the range of 0.904-9.04 microg (r = 0.999 9), and 1.27-12.7 microg (r = 0.999 9) respectively and the average recoveries of echinacoside (and acteoside) were 98.9% (n = 5, RSD 1.9%), and 97.0% (n = 5, RSD 0.97%)., Conclusion: The method is simple, quick, acurate. In all of the samples, the contents of echinacoside is markedly more than that of acteoside, the content of the two active component in the wilding is higher than that in the planting. The content of sample in the different sizes gradually increase from the big to the small, and the contents of samples collected from the different hosts vary markedly. These results are useful for the quality evaluation of medicinal materials of C. tubulosa.
- Published
- 2005
824. [Effects of mineral cations on the accumulation of tanshinone II A and protocatechuic aldehyde in the adventitious root culture of Salvia niltiorrhiza].
- Author
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Guo XH, Gao WY, Chen HX, and Huang LQ
- Subjects
- Abietanes, Copper pharmacology, Magnesium pharmacology, Plant Roots growth & development, Plant Roots metabolism, Plants, Medicinal growth & development, Plants, Medicinal metabolism, Salvia miltiorrhiza metabolism, Tissue Culture Techniques, Zinc pharmacology, Benzaldehydes metabolism, Catechols metabolism, Iron pharmacology, Manganese pharmacology, Phenanthrenes metabolism, Salvia miltiorrhiza growth & development
- Abstract
Objective: To study the effects of mineral cations on the growth of Salvia niltiorrhiza adventitious roots and the accumulation of tanshinone II A and protocatechuic aldehyde., Method: The adventitious roots were cultured under different concentrations of metal cations and the contents of tanshinone II A and protocatechuic aldehyde were determined by HPLC., Result: Fe2 + and Mn2+ in MS medium are suitable for adventitious root growth, but Cu2+ and Mg2+ in high concentration could improve root proliferation, and Zn2+ has no obvious effect on root growth. As for tanshinone II A and protocatechuic aldehyde biosynthesis, Cu2+ and Zn2+ would inhibit the biosynthesis of protocatechuic aldehyde, furthermore, proper Fe2+ and Mg2+ concentration could advance the biosynthesis of protocatechuic aldehyde, whereas, Mn2+ in high concentration could accelerate the biosynthesis of protocatechuic aldehyde. Cu2+, Zn2+, Mg2+ in low concentration and Fe2+ and Mn2+ in high concentration would all advance the biosynthesis of tanshinone II A., Conclusion: The mineral cations have obvious effects on the secondary metabolites biosynthesis in adventitious root culture of S. niltiorrhiza.
- Published
- 2005
825. [Influence of processing rhubarb on it's anthraquinone contents].
- Author
-
Li XD and Huang LQ
- Subjects
- Drugs, Chinese Herbal isolation & purification, Emodin analogs & derivatives, Emodin analysis, Hot Temperature, Plant Roots chemistry, Quality Control, Reproducibility of Results, Anthraquinones analysis, Drugs, Chinese Herbal chemistry, Plants, Medicinal chemistry, Rheum chemistry, Technology, Pharmaceutical methods
- Abstract
Objective: To investigate the influence of processing of Radix et Rhizoma Rhei on it's five anthraquinone components, and to develop a HPLC quantatitive method for these components in Radix et Rhizoma Rhei., Method: Chromatographic conditions are as follow: Kromasil-C18 (4.6 mmx 150 mm, 5 microm) column, ethanol -H2O-H3PO4 (85:15:0.1) as mobile phase and the detection wavelength at 254 nm., Result: The average recoveries are 97.9% (aleo-emodin), 97.1% (rhein), 97.6% (emodin), 97.4% (chrysophanol) and 99.1% (physcion). RSD are 1.4%, 1.1%, 0.9%, 1.1% and 2.2% correspondingly. After processing, the contents of five anthraquinone aglycones descended compared with the crude material, the descending percentage are 15.9% (aleo-emodin), 28.0% (rhein), 25.8% (emodin), 10.0% (chrysophanol) and 10.3% (physcion)., Conclusion: This method is simple, sensitive and repeatable. It' canbeased for quality control of Radix et Rhizoma Rhei and it's processed products.
- Published
- 2005
826. [Habitat characteristics for the growth of Atractylodes lancea based on GIS].
- Author
-
Guo LP, Huang LQ, Yan H, Lv DM, and Jiang YX
- Subjects
- Atractylodes chemistry, China, Oils, Volatile analysis, Plants, Medicinal chemistry, Rain, Temperature, Atractylodes growth & development, Climate, Ecosystem, Geographic Information Systems, Plants, Medicinal growth & development
- Abstract
Objective: To study the habitat characteristics for the growth of Atractylodes lancea., Method: The leading factors for the growth of A. lancea are determined after stepwise regression, and the limiting factor are determined by field study, literature search,and weather records. The mean values of leading factors and the limiting factors of A. lancea for 30 years were applied to do spatial interpolation using IDIRIEIW softwares. An overlay was prerformed using data obtained in Mt. Maoshan and the general habitat of A. Iancea., Result: It was found that precipitation is one of the dominant climate factors that affects the quality of A. lancea. High temperature was one of the limiting factor which influences the living of A. lancea. The best habitat was determined to be: annual mean temperature more than 15 degrees C, monthly mean minimum temperature between - 2- -1 degrees C, monthly mean maximum temperature about 32 degrees C, lowest tolerable temperature - 17- -15 degrees C, drought season 1 - 2 months, annual mean precipitation between 1000 - 1160 mm., Conclusion: Mt. Maoshan was characterized by having high temperature, short drought season and adequate precipitation. The growth of A. Lancea is a result of environmental stress.
- Published
- 2005
827. [Determination of carvacrol and thymol in Mosla chinensis by HPLC].
- Author
-
Ji L, Wang F, Liu YY, Tong Y, Li XD, Feng XF, Huang LQ, and Zhou GP
- Subjects
- Chromatography, High Pressure Liquid, Cymenes, Plant Components, Aerial chemistry, Quality Control, Lamiaceae chemistry, Monoterpenes analysis, Plants, Medicinal chemistry, Thymol analysis
- Abstract
Objective: To establish a quantitative method of determination of carvacrol and thymol in Mosla chinensis., Method: The sample was extracted with 95% ethanol, ODS column was used with methanol-water-acetic acid (60:40:2) as mobile phase. The detection wavelength was set at 274 nm., Result: The linearities of carvacrol and thymol were respectively in the range of 0.23-2.15 microg (r = 0.9999) and 0.39-2.36 microg (r = 0.9999); the average recoveries were 99.9% (RSD 1.4%) and 98.6% (RSD 1.3%); the RSD of repeatability were 1.1% and 1.6%., Conclusion: The method is reliable, and can be used for quality control of M. chinensis.
- Published
- 2004
828. [Genetic analysis of cultivated ginseng population with the assistance of RAPD technology].
- Author
-
Shao AJ, Li X, Huang LQ, Wei JH, and Lin SF
- Subjects
- Cluster Analysis, DNA Primers genetics, Genetic Variation, Panax classification, Panax growth & development, Phylogeny, Plants, Medicinal growth & development, Random Amplified Polymorphic DNA Technique methods, DNA, Plant genetics, Panax genetics, Plants, Medicinal genetics
- Abstract
Objective: To provide reference for breeding of cultivated ginseng by researching the genetic diversities among different strains., Method: To make up the systematic diagram of genetic relationship by calculating the proximity coefficient with SPSS 10.0 software and clustered by between-groups linkage method., Result: The selected 18 random primers used in PCR amplification to produce 145 bands in 17 samples, among which 53 bands (36.5%) are polymorphic. The results of cluster analysis show that the genetic relationship between different strains of Da-maya is closer than that of Er-maya, which proves that it is feasible to differentiate the cultivated groups in molecular level on outside character., Conclusion: RAPD is a favorable molecular marker to assist ginseng breeding.
- Published
- 2004
829. [Study on genetic diversity of herba Cistanches by RAPD].
- Author
-
Cui GH, Chen M, Huang LQ, Xiao SP, and Li D
- Subjects
- Cistanche classification, DNA, Plant genetics, Genetic Variation, Genetics, Population, Random Amplified Polymorphic DNA Technique, Cistanche genetics, Plants, Medicinal genetics, Polymorphism, Genetic
- Abstract
Objective: To determine the genetic diversity of Cistanche species., Method: Two populations of Cistanche deserticola and four populations of C. tubulosa were analyzed by random amplified polymorphic DNA (RAPD) markers., Result: A total of 76 and 87 loci were amplified using 10 random primers each other. The average percentage of polymorphic loci of C. deserticola was 47.37%. The PPL were 39.47% and 35.53% for two populations. Average Nei's gene diversity was 0.1358, Shannon' s genetic diversity was 0.2072, and Gst was 0.2546. The average PPL of C. tubulosa was 27.59%. It was 19.54% to 25.29% in different populations and Andi'er population had the highest. Average Nei's gene diversity was 0.0823, and Shannon' s genetic diversity was 0.125 8, Cst was 0.175 5., Conclusion: The diversity of Cistanche deserticola is higher than that of C. tubulosa, but both has differentiation among populations, C. deserticola has already separated itself into two different ecotypes.
- Published
- 2004
830. [Ecological research on resources of Chinese herbal medicine].
- Author
-
Guo LP and Huang LQ
- Subjects
- China, Conservation of Natural Resources, Geographic Information Systems, Pharmacognosy, Quality Control, Ecosystem, Plants, Medicinal
- Abstract
Objective: To explore the status and development of the ecological research on resources of Chinese herbal medicine., Method: The ecological domain related to the resources of Chinese Herbal Medicine was Analyzed., Result: It was found that the weakness of the theoretical study and application were the main problems constrained the development of ecological research of Chinese Herbal Medicine., Conclusion: A new subject, ecology of resources of Chinese herbal medicine, will come into being with the development of the theoretical and practical study and application of new technology and method.
- Published
- 2004
831. [Determining the quantity of hypericin in medicinal materials and asepsis seedings of Hypericum perforatum by HPLC].
- Author
-
Chen ML, Huang LQ, Yang B, Fu MH, and Luo YM
- Subjects
- Anthracenes, China, Chromatography, High Pressure Liquid methods, Ecosystem, Hypericum genetics, Perylene analysis, Perylene isolation & purification, Plants, Medicinal genetics, Quality Control, Seasons, Hypericum chemistry, Perylene analogs & derivatives, Plants, Medicinal chemistry
- Abstract
Objective: To establish the method of determining the quantity of hypericin in Hypericum perforatum and determine the quantity of the hypericin in defferent medicinal materials and asepsis seedings which grow in defferent environment., Method: The specimen is extracted with methanol--Pyridine (9:1) ultrasound extraction. Chromatographic assay is performed on a hypersily ODS2 (4.6 mm x 150 mm, 5 microm) column. The mobile phase is composed of methanol -1.56% dihydric natrium phosphate hydrogen natrium solution (shift solution's acidity to 2.1 with phosphoric acid)--ethyl acetate (4:1.9:1), velocity of flow is 1 mL x min(-1); column temperature is 35 degrees C; the detection wavelength is 590 nm., Result: A satisfactory seperaration between hypericin and impurity. The calibration curve is linear over the range of 0.0524-0.2620 microg for hypericin (r = 0.9998). The average recovery of hypericin is 97.50%., Conclusion: The quantity of hypericin in Hypericum perforatum has something to do with the genetic factor, environment factor, growing period and dry means. The method of determining the quantity of hypericin can be regarded as the method of controling the quantity of medicinal materials.
- Published
- 2003
832. [Effect of factors on callus biomass and synthetic mass of hypericin in Hypericum perforatum].
- Author
-
Xu MS, Huang LQ, Chen ML, Ye HC, and Li GF
- Subjects
- Anthracenes, Culture Media, Hypericum metabolism, Mannose pharmacology, Perylene metabolism, Plants, Medicinal metabolism, Povidone analogs & derivatives, Povidone pharmacology, Tissue Culture Techniques, Hypericum growth & development, Nitrogen pharmacology, Perylene analogs & derivatives, Plants, Medicinal growth & development
- Abstract
Objective: To study the effect of several factors on the quantity of hypericin in H. perforatum callus., Method: High efficiency liquid phase chromatography and plant tissue culture were applied., Result and Conclusion: When the ratio of nitro-nitrogen to amina-nitrogen is 3:1, the callus biomass is 1.6-fold and the synthetic mass of hypericin rises. 0.1-0.20 mg x L(-1) mannose improves the content of total hypericin. The addition of PVP or PVPP can promote improvement of the growth and biosynthesis of callus.
- Published
- 2003
833. [Prospects and application of electrochemical analysis to the study of natural antioxidants].
- Author
-
Yang B and Huang LQ
- Subjects
- Animals, Electrochemistry, Enzyme Inhibitors isolation & purification, Enzyme Inhibitors metabolism, Enzyme Inhibitors pharmacology, Flavonoids isolation & purification, Flavonoids metabolism, Flavonoids pharmacology, Free Radical Scavengers isolation & purification, Free Radical Scavengers metabolism, Free Radical Scavengers pharmacology, Humans, Lipid Peroxidation drug effects, Vegetables chemistry, Antioxidants isolation & purification, Antioxidants metabolism, Antioxidants pharmacology, Plants, Medicinal chemistry
- Published
- 2002
834. [The naphtha composing characteristics of geoherbs of Atractylodes lancea].
- Author
-
Guo LP, Liu JY, Ji L, and Huang LQ
- Subjects
- Atractylodes classification, Ecosystem, Furans analysis, Gas Chromatography-Mass Spectrometry, Oils, Volatile isolation & purification, Plants, Medicinal classification, Sesquiterpenes, Spiro Compounds analysis, Terpenes analysis, Atractylodes chemistry, Oils, Volatile chemistry, Plant Extracts analysis, Plants, Medicinal chemistry, Sesquiterpenes, Eudesmane
- Abstract
Objective: To find the chemical diversity and characteristics of A. lancea on two levels--individuals and populations, and to discover the chemical essentials for forming geoherbs., Method: 47 rhizomes of A. lancea were collected in 7 populations, and 6 naphtha components (1. elemol, 2. hinesol, 3. beta-eudesmol, 4. atractylone, 5. atractylodin, 6. atractylenolid I) in the rhizomes were determined by GC-MS combination. Principal Component Analysis and Cluster Analysis were carried out by SPSS., Result: Cluster Analysis of the 6 main components indicated that the chemical components of geoherbs were different from those of the non-geonerbs of A. lancea. Other analysis showed as follows: 1. The general oil of geoberbs were lower than that of non-geoherbs(P < 0.01), but components yielding more than 1% (% of the total oil) were more than non-geoherbs(P < 0.01); 2. Hinesol mixing beta-eudesmol was more in non-geoherbs, which atractylodin mixing atractylone was more in geoherbs(P < 0.001); 3. Principal Component Analysis implied that atractylone was the most important component to discriminate geoherbs and non-geoherbs of A. Lancea., Conclusion: The naphtha composing characteristics of geoherbs was the special proportionment sale, viz. atractylone: hinesol: beta-eudesmol: atractylodin being(0.70~2.00):(0.04~0.35):(0.09~0.40):1.
- Published
- 2002
835. [RAPD analysis on different varieties of Rehmannia glutinosa].
- Author
-
Cheng JL, Huang LQ, Shao AJ, and Lin SF
- Subjects
- Plant Leaves genetics, Polymorphism, Genetic, Random Amplified Polymorphic DNA Technique, DNA, Plant genetics, Plants, Medicinal genetics, Rehmannia genetics
- Abstract
Objective: To provide molecular evidences for its breeding by studying the genetic relationship among varieties of Rehmannia glutinosa., Method: Nineteen varieties were detected by Randomly Amplified Polymorphic DNA(RAPD) markers., Result: The 20 selected primers produced 163 bands, among which 114(69.9%) were polymorphic. A DNA molecular dendrogram was established based on Hierarchical cluster analysis of 163 DNA bands amplified by 20 primers, which divided the 19 varieties into four groups: Group Beijing, Group 85-5, Group Guolimao and the other Group., Conclusion: 8 varieties of Group Beijing have a close genetic relationship, and so have varieties of Group 85-5, which provides information for Rehmannia glutinosa's breeding.
- Published
- 2002
836. [The influences of inorganic elements in soil on the geolism of Atractylodes lancea].
- Author
-
Guo LP, Huang LQ, and Yan YN
- Subjects
- Calcium analysis, Chromium analysis, Fluorocarbons analysis, Geologic Sediments analysis, Iron analysis, Rhizome chemistry, Atractylodes chemistry, Nickel analysis, Plants, Medicinal chemistry, Soil analysis
- Abstract
Objective: To study the influence of inorganic elements in soil on the geolism of Atractylodes lancea., Method: The concentrations of 15 kinds of inorganic elements of regional A. lancea and their correspondingly soils were determined, and the data were analysed with the SPSS10.0 software., Result: The mean concentration of Ni in geoherbs of A. lancea was 3 times higher than that in the non-geoherbs. The accumulation of Ca is high in geoberbs. There is no relationship between the concentrations of inorganic elements in A. lancea and those in their corresponding soil., Conclusion: A. lancea adjusts the concentration of inorganic elements mainly by active absorption, and there are good relationship between the accumulation of Fe and Cr, Fe and Hg, Hg and Cr, As and Pb, Co and Sr.
- Published
- 2002
837. [Study on complex system of Chinese materia medica GAP fulfilling].
- Author
-
Huang LQ, Cui GH, and Dai RW
- Subjects
- Delphi Technique, Ecology, Fertilizers, Quality Control, Total Quality Management, Drugs, Chinese Herbal economics, Plants, Medicinal growth & development
- Abstract
Objective: To provide new model of thinking and management for Chinese Meteria Medica GAP fulfilling., Method: After analyzing the problems of GAP fulfilling, and considering GAP a complex system, we had some initial discussions about the systematic structure, characteristics and the methods for fulfilling it., Result: The GAP was a complex system which consisted of biological system, environment system and management system with its own characteristics and methods., Conclusion: The GAP fulfilling still has some problems and needs the direction of systematic opinion of complex system.
- Published
- 2002
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