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In this study low-input RNA-sequencing was used to annotate the molecular identity of endothelial cells isolated and immunopurified with CD144 microbeads. Using this technique, comparative gene expression profiling from healthy subjects and patients with type 2 diabetes mellitus identified both known and novel pathways linked with EC dysfunction. Modeling of diabetes by treating cultured ECs with high glucose identified shared changes in gene expression in diabetic cells. Overall, the data demonstrate how purified ECs from patients can be used to generate new hypotheses about mechanisms of human vascular disease.

The goal of our research was to assess the effect of Enrofloxacin (fluoroquinolone antibacterial drug) on WBC indices of different kinds of poultry at young age. In order to study the dynamics we performed two series of experiments employing groups as follows: Group 1 – control group (one-day-old chicks, receiving pure drinking water), Group 2 – experimental group (one-day-old chicks, receiving Enrofloxacin via drinking water at concentration 200 g/L for 10 consecutive days), Groups 3 and 4 were made with one-day-old ducklings, the experiment design was the same as with chicks. Blood samples were drawn from every bird on Day 1, Day 3, Day 5, Day 7 and Day 9 after the drug withdrawal. We performed differential leukocyte count, leukogram was made and we also calculated leukocyte (hematological) indices. The data analysis showed reliable changes of blood indices both in chicks and in ducklings. The greatest changes in the results of both experimental groups as compared to the control groups were observed on Day 7, but they were not lasting. We observed more pronounced and lasting changes in the leukogram of Group 2, while duckling blood showed reliable changes of leukocyte indices. But all in all dynamics in WBC indices that we observed did not show toxic effect of the drug on bird organism.

The prothrombotic and proinflammatory properties of lipoprotein(a) (Lp(a)) have been hypothesized to play a role in the pathogenesis of severe COVID-19; however, the prognostic impact of Lp(a) on the clinical course of COVID-19 remains controversial. This study aimed to investigate whether Lp(a) may be associated with biomarkers of thrombo-inflammation and the occurrence of thrombotic events or adverse clinical outcomes in patients hospitalized for COVID-19. We consecutively enrolled a cohort of patients hospitalized for COVID-19 and collected blood samples for Lp(a) assessment at hospital admission. A prothrombotic state was evaluated through D-dimer levels, whereas a proinflammatory state was evaluated through C-reactive protein (CRP), procalcitonin, and white blood cell (WBC) levels. Thrombotic events were marked by the diagnosis of deep or superficial vein thrombosis (DVT or SVT), pulmonary embolism (PE), stroke, transient ischemic attack (TIA), acute coronary syndrome (ACS), and critical limb ischemia (CLI). The composite clinical end point of intensive care unit (ICU) admission/in-hospital death was used to evaluate adverse clinical outcomes. Among 564 patients (290 (51%) men, mean age of 74 ± 17 years) the median Lp(a) value at hospital admission was 13 (10–27) mg/dL. During hospitalization, 64 (11%) patients were diagnosed with at least one thrombotic event and 83 (15%) patients met the composite clinical end point. Lp(a), as either a continuous or categorical variable, was not associated with D-dimer, CRP, procalcitonin, and WBC levels (p > 0.05 for all correlation analyses). In addition, Lp(a) was not associated with a risk of thrombotic events (p > 0.05 for multi-adjusted odds ratios) nor with a risk of adverse clinical outcomes (p > 0.05 for multi-adjusted hazard ratios). In conclusion, Lp(a) does not influence biomarkers of plasma thrombotic activity and systemic inflammation nor has any impact on thrombotic events and adverse clinical outcomes in patients hospitalized for COVID-19. [ABSTRACT FROM AUTHOR]

This study included the definition of over crowding in traditional settlements, confinement of animals and not going out to graze in natural pastures which causes stress and its impact on the characteristics and standards in the blood. Metabolism for two weeks by oral dosing of 3 g, 6 g and control group and we found that the best use of a concentration of 3 g gives good results for adaptation and changes the number of white blood cells, as there was a significant increase in stressed animals when adding vitamin C and the variation decreased significantly(P < 0.05) for the lymphocytes and the value of granulocytes increased, significant (P < 0.05) differences were found between the values and the number of granulocytes, lymphocytes in a good measure to indicate changes caused by environmental stress, overcrowding and there is a significant increase in the values number of red blood cells and that vitamin C will enhance immunity and the ability to resist and adaptation. [ABSTRACT FROM AUTHOR]

Chronic cigarette smoking is a major risk factor for many serious diseases. While complete cessation of smoking is the best option to reduce harm from smoking, adverse impacts of smoking on health could persist for several years after cessation. Therefore, Biomarkers of Potential Harm (BoPH) are useful in interim evaluations of the beneficial effects of smoking cessation or switching to potentially lower-risk tobacco products. A 14-day smoking abstinence study was conducted under clinical confinement conditions and enrolled 70 subjects into younger (24–34 years, n = 33) and older (35–60 years, n = 37) age cohorts. Biomarkers of Exposure (BoE), which indicate exposure to nicotine and other toxicants, were measured at baseline, 7 and 14 days. Several BoPH including previously identified eicosanoids (leukotriene 4 (LTE4) and 2,3-dinor thromboxane 2 (2,3-d-TXB2) and others were evaluated. Significant declines in BoE, LTE4, 2,3-d-TXB2, neutrophils, WBC and select RBC, and arterial blood gas parameters were observed in both age cohorts at Days 7 and 14 compared to baseline, while other BoPH (e.g., FeNO) showed age-related effects. Rapid and reproducible reductions in LTE4, 2,3-d-TXB2 WBC, and neutrophil counts were consistently detected following smoking abstinence, indicating the value of these markers as useful BoPH. [ABSTRACT FROM AUTHOR]

Background: Infections of cerebrospinal fluid (CSF), peritoneal and pleural fluids often result in severe morbidity and mortality. Cytometric analysis, cell count, and cell differentiation is supportive in rapid clinical decision. The aim of this study is to evaluate the performance of Sysmex UF-5000 (Sysmex Co., Kobe, Japan) in body fluid (BF) mode for white blood cells and bacterial counting and compare the results with manual microscopy, gram staining, and culture as reference method. Methods: A total of 634 body fluid samples (433 CSF, 100 pleural, and 101 peritoneal fluid) submitted to our clinical microbiology laboratory between December 2018 and January 2020 were enrolled in the study. Specimens were analyzed for white blood cell (WBC) counts with Sysmex UF-5000-BF mode. The results were grouped into five categories and compared with the conventional methods as reference method. The relationship between culture results and Sysmex UF5000-BF bacterial load and WBC count were evaluated for the detection of infection. Results: The categorical agreement of UF 5000-BF mode was very strong for CSF (correlation coefficient: 0.70, p < 0.05) and good agreement was found for pleural and peritoneal samples (correlation coefficient: 0.63, p < 0.05). Sensitivity was 100% and specificity was 64% in pleural samples. For ascites sensitivity and specificity were found as 94.1% and 77.4%, and for CAPD samples as 100% and 81,2% respectively. Bacterial and WBC count with flow cytometry was higher among culture positive samples (p < 0.05). The same significant difference was detected between the UF 5000-BF bacterial counts and the samples in which bacteria were detected in the gram preperation (p < 0.05). WBC count by UF-5000-BF was also higher among culture positive compared to culture negative samples (p < 0.05). Conclusions: Our results demonstrated that the flow cytometric method, Sysmex UF-5000 body fluid (BF) mode, can be an alternative particularly in CSF samples. Detection of 100% sensitivity in peritoneal and pleural samples can prevent unnecessary antibiotic treatment and possible resistance development. Besides, automated bacterial counting can be used for rapid prediction of a positive body fluid culture. [ABSTRACT FROM AUTHOR]

During the COVID-19 pandemic, different SARS-CoV-2 variants of concern (VOC) with specific characteristics have emerged and spread worldwide. At the same time, clinicians routinely evaluate the results of certain blood tests upon patient admission as well as during hospitalization to assess disease severity and the overall patient status. In the present study, we searched for significant cell blood count and biomarker differences among patients affected with the Alpha, Delta and Omicron VOCs at admission. Data from 330 patients were retrieved regarding age, gender, VOC, cell blood count results (WBC, Neut%, Lymph%, Ig%, PLT), common biomarkers (D-dimers, urea, creatinine, SGOT, SGPT, CRP, IL-6, suPAR), ICU admission and death. Statistical analyses were performed using ANOVA, the Kruskal–Wallis test, two-way ANOVA, Chi-square, T-test, the Mann–Whitney test and logistic regression was performed where appropriate using SPSS v.28 and STATA 14. Age and VOC were significantly associated with hospitalization, whereas significant differences among VOC groups were found for WBC, PLT, Neut%, IL-6, creatinine, CRP, D-dimers and suPAR. Our analyses showed that throughout the current pandemic, not only the SARS-CoV-2 VOCs but also the laboratory parameters that are used to evaluate the patient's status at admission are subject to changes. [ABSTRACT FROM AUTHOR]

Objective: Comparative effect of Curcuma longa (C. longa) ethanolic extract and curcumin on paraquat (PQ)-induced systemic and lung oxidative stress and inflammation were evaluated in the present study.Materials and Methods: Control animals were exposed to normal saline and PQ group to 54 mg/m3 PQ aerosols (8 times, each time for 30 min). Treatment groups were exposed to PQ and treated with 150 and 600 mg/kg/day C. longa, or 30 and 120 mg/kg/day curcumin after PQ exposure period for 16 days. Total and differential white blood cells (WBC) and oxidative markers were measured both in bronchoalveolar lavage (BALF) and blood at the end of the study.Results: Total and differential WBC counts as well as malondialdehyde (MDA) level were significantly increased but total thiol content and the activities of catalase (CAT) and superoxide dismutase (SOD) were reduced in both the BALF and blood of the PQ group in comparison with the control group (p

Abstract Background The development of tools that could help emergency department clinicians recognize stroke during triage could reduce treatment delays and improve patient outcomes. Growing evidence suggests that stroke is associated with several changes in circulating cell counts. The aim of this study was to determine whether machine-learning can be used to identify stroke in the emergency department using data available from a routine complete blood count with differential. Methods Red blood cell, platelet, neutrophil, lymphocyte, monocyte, eosinophil, and basophil counts were assessed in admission blood samples collected from 160 stroke patients and 116 stroke mimics recruited from three geographically distinct clinical sites, and an ensemble artificial neural network model was developed and tested for its ability to discriminate between groups. Results Several modest but statistically significant differences were observed in cell counts between stroke patients and stroke mimics. The counts of no single cell population alone were adequate to discriminate between groups with high levels of accuracy; however, combined classification using the neural network model resulted in a dramatic and statistically significant improvement in diagnostic performance according to receiver-operating characteristic analysis. Furthermore, the neural network model displayed superior performance as a triage decision making tool compared to symptom-based tools such as the Cincinnati Prehospital Stroke Scale (CPSS) and the National Institutes of Health Stroke Scale (NIHSS) when assessed using decision curve analysis. Conclusions Our results suggest that algorithmic analysis of commonly collected hematology data using machine-learning could potentially be used to help emergency department clinicians make better-informed triage decisions in situations where advanced imaging techniques or neurological expertise are not immediately available, or even to electronically flag patients in which stroke should be considered as a diagnosis as part of an automated stroke alert system.

Low patient motivation and relatively long procedures can sometimes limit the gains of the PAP test. At this point, the clinician may need additional stimulating tests to persuade patients to perform screening tests. The aim of this study was to determine the predictive value of hematological parameters for the high-grade squamous intraepithelial lesion (HG-SIL). Ninety-one patients possessing HG-SIL with complete blood counts and 111 healthy patients with normal cervical cytology were reviewed retrospectively. The age, WBC, and RBC count were significantly different in patients with HG-SIL than in controls. A cutoff value was found 42.5 for age (42% sensitivity and 58 % specificity, AUC: 0.604), 8.65 for WBC (44% sensitivity and 68% specificity, AUC: 0.570), and 4.47 for RBC (65% sensitivity and 58% specificity, AUC: 0.628) in ROC curve analysis. The results of the study show that the age of the patients, RBC, and WBC counts are important in precancerous intraepithelial lesions. Clinicians should warn female patients in the risk group to have regular gynecological examinations and screenings. [Med-Science 2022; 11(2.000): 820-4]

BackgroundClinicians often use population-based reference intervals (RIs) when interpreting patient results. However, this method can present problems if the analyte in question has wide variability from person to person.MethodsWe examined the biological variation of routine hematologic markers in 82 white non-Hispanic men every 6 months during a 30-year period, to determine the usefulness of population-based RIs and age-related decline of hematological markers.ResultsMany of these markers showed significant person-to-person differences (index of individuality

The present study aimed to determine the effect on different blood parameters (WBCs, RBC, HGB, HCT, MCV, MCH, and MCHC) in fingerlings of common carp (Cyprinus carpio) weighing 13.5 ± 1 g with pomegranate peels (Punica granatum) in their different forms, raw (PPR), alcoholic (PPA) and water (PPW), which were added to their feed as 0.5% and 1% respectively, in addition to the control treatment (21 replicates). A commercial diet was used containing 35% crude protein, 6% lipid, 12% Ash and 50 TVN for ten weeks. At the end of the experiment, blood was drawn from the heart of the fish, and analyses were performed. The results showed that RBCs, HGB and HCT improved significantly (P< 0.05), while there were no significant differences (P> 0.05) in WBCs, MCH, and MCHC. In short, we recommend adding pomegranate peels or extracts (alcoholic or aqueous) to common carp food at a rate not exceeding 1%.

Whole-body cryotherapy (WBC) has been reported to maximize physical recovery after exercise and reduce the ensuing muscle damage. In addition, WBC triggers cardiovascular responses leading to an increased vagal drive. Here we tested whether WBC may boost exercise performance as well as post-exercise recovery. Moreover, we compared the effects of WBC and exercise on sympathovagal balance and tested whether these two factors may interact. ECG was recorded in 28 healthy adults who underwent rest, all-out effort on a cycloergometer, 5 min recovery and again rest. After 3–5 days, WBC (3 min exposure to − 150 °C air) was applied and the whole procedure repeated. Total exercise duration was split into the time needed to reach peak power output (tPEAK) and the time to exhaustion (tEXH). The post-exercise exponential decay of HR was characterized by its delay from exercise cessation (tDELAY) and by its time constant (τOFF). Sympathovagal balance was evaluated by measuring HR variability power in the low (LF) and high (HF) frequency bands, both before exercise and after recovery from it. Sympathetic vs. vagal predominance was assessed by the sympathovagal index LFnu. Paired t-tests indicated that WBC increased tEXH and reduced tDELAY, speeding up the HR recovery. These results suggest that WBC may be exploited to boost exercise performance by about 12–14%. ANOVA on HR variability confirmed that exercise shifted the sympathovagal balance towards sympathetic predominance, but it also highlighted that WBC enhanced vagal drive at rest, both before exercise and after full recovery, covering ~ 70% of the exercise effect. [ABSTRACT FROM AUTHOR]

The clinical course of acute pancreatitis (AP) can be variable depending on the severity of the disease, and it is crucial to predict the probability of organ failure to initiate early adequate treatment and management. Therefore, possible high-risk patients should be admitted to a high-dependence unit. For risk assessment, we have three options: (1) There are univariate biochemical markers for predicting severe AP. One of their main characteristics is that the absence or excess of these factors affects the outcome of AP in a dose-dependent manner. Unfortunately, all of these parameters have low accuracy; therefore, they cannot be used in clinical settings. (2) Score systems have been developed to prognosticate severity by using 4–25 factors. They usually require multiple parameters that are not measured on a daily basis, and they often require more than 24 h for completion, resulting in the loss of valuable time. However, these scores can foresee specific organ failure or severity, but they only use dichotomous parameters, resulting in information loss. Therefore, their use in clinical settings is limited. (3) Artificial intelligence can detect the complex nonlinear relationships between multiple biochemical parameters and disease outcomes. We have recently developed the very first easy-to-use tool, EASY-APP, which uses multiple continuous variables that are available at the time of admission. The web-based application does not require all of the parameters for prediction, allowing early and easy use on admission. In the future, prognostic scores should be developed with the help of artificial intelligence to avoid information loss and to provide a more individualized risk assessment. [ABSTRACT FROM AUTHOR]

Background: Metformin, an anti-hyperglycemic drug, treats type 2 diabetes with diet. It increases muscle glucose consumption, maintains blood levels, and does not cause severe hypoglycemia. Aim: To evaluate the effect of metformin on some plasminogen and cortisol variables and some hematological variables represented in the percentage of compact red blood cells, hemoglobin concentration, total number of red blood cells, and white blood cells in patients with type 2 diabetes. Subjects and Method: 90 patients with type 2 diabetes and patients who used metformin treatment twice a day continued for three months, six months, and one year were included, according to a questionnaire. After confirming the status of type 2 diabetes through laboratory tests, samples were taken. They were divided into four groups according to the period used in the treatment. Results: The results indicated that treatment with metformin led to a significant decrease in the level of Plasminogen Activator-1 (PAI-1) and cortisol hormones, in addition, to the decline in hematological indices, packed cell volume (PCV), hemoglobin (Hb), red blood cells (RBCs) and white blood cells (WBCs) within works 2, 3 and 4 compared to the control group. Conclusion: Metformin may improve the risk of atherosclerotic disease that may occur due to diabetes by lowering blood glucose, such as improving endothelial function, decreasing inflammatory activity, and decreasing blood parameters during treatment, which may be related to anemia. [ABSTRACT FROM AUTHOR]

Giardia lamblia, a protozoan parasite featuring flagella .and binucleation, is capable of infecting a diverse range of mammalian hosts. The incidence of human giardiasis, a veritable cosmopolitan pathogen, is most pronounced in developing nations. The objective of the present study was to evaluate the impact of an infection caused by the Giardia lamblia parasite on iron levels and various hematological parameters in patients. the study conducted between August 2022 and February 2023, blood samples were collected from people who were infected and non-infected with Giardia lamblia to evaluate the element iron and some blood indicators and the extent to which they were affected by infection. 60 blood samples (36 males and 24 females) were taken from people infected with Giardia, and 30 blood samples (15 males and 15 females) were taken from healthy people (controls). The infection was diagnosed by a direct smear and cultured in the laboratory. The control group underwent both clinical and laboratory examinations to confirm the absence of any disease. The findings of the present investigation exhibit a noteworthy reduction in the average levels of serum iron (SI)., mean number of red blood cells (RBC), hemoglobin (Hb), and percentage of the packed cell volume (PCV) in people with the disease compared to healthy subjects, while a significant increase in the mean of the total number of white blood cells (WBC) was recorded in patients compared to healthy subjects. Individuals with Giardia labmlia may exhibit notable changes in several hematological factors. Routine assessment of hematological factors is necessary for timely detection and effective treatment of giardiasis. [ABSTRACT FROM AUTHOR]

Background: To evaluate the usefulness of immature to total neutrophil ratio (I/T Ratio) in early neonatal sepsis diagnosis. Materials & methods: A total of 115 neonates included 4-5 days old who were admitted to neonatal intensive care unit (NICU) and suspected to have sepsis based on peri-natal risk factors which included low birth weight, prematurity, abnormal amniotic fluid, premature rupture of membranes, foetal distress and maternal pyrexia together with clinical features were taken as the study group. The unpaired student t test will be used to determine the statistical significance between the study groups. A P value of <0.05 will be considered to be statistically significant. Results: The difference of mean positive blood culture was significantly higher in I/T ratio, and WBCs as compared negative blood culture. p value were 0.001 &<0.0001 respectively. Although, the mean weight with positive blood culture 2.451 ± 0.456 kg was slightly more as compared to negative BC 2.391 ± 0.714 kg but it was statistically non-significant. The mean I/T in positive CRP 0.204 ± 0.04 was non-significantly higher as compared to negative CRP 0.162 ± 0.038 (p =0.074). Conclusion: The I/T ratio is a useful test for detecting early sepsis (EOS) with high specificity. [ABSTRACT FROM AUTHOR]

Simple Summary: Eosinopenia has been used as a biomarker of systemic inflammatory response syndrome in critically ill humans. Horses are extremely prone to developing systemic inflammation in different conditions such as endotoxemia. It is for that reason that new biomarkers are needed in horses to rapidly identify the patients that require hospitalization in the intensive care units to minimize unnecessary expenses. The aim of this study was to evaluate eosinopenia as a potential marker of systemic inflammation and prognosis in horses. The results showed lower eosinophil counts in horses affected with systemic inflammation compared to the control group (p < 0.05). Horses with eosinopenia were less likely to survive, and hence, eosinophil count could be used as a marker of prognosis and disease. Systemic inflammatory response syndrome (SIRS) is a very common finding in critically ill patients. To accurately identify patients with SIRS and those who need intensive care, several markers have been evaluated, including cortisol, WBC or lactate. It is widely known that a stress leukogram includes eosinopenia as one of its main markers (neutrophilia, eosinopenia, lymphopenia and mild monocytes). It is known that cortisol concentration in plasma is the main stress biomarker and is strongly correlated with the severity of disease in horses. However, it is not possible to measure this parameter routinely in clinical conditions. Hence, in this study it was hypothesized that the eosinophil count could be a reliable parameter to identify critically ill horses. Horses included in this study were divided into three groups: Group A (sick horses received at the Emergency Unit which did not fulfil the criteria for SIRS), Group B (horses that meet two or more criteria for inclusion in the definition of SIRS) and a control group of healthy horses. In this study, horses with SIRS showed lower eosinophil counts than healthy horses. Moreover, non-surviving horses exhibited lower eosinophil counts than survivors. Eosinopenia could be used to identify horses with SIRS and can be useful as a prognostic marker. [ABSTRACT FROM AUTHOR]

Background: The incidence of Clostridioides difficile infection (CDI) has increased in recent years in patients with inflammatory bowel disease (IBD). C. difficile is a toxin-producing bacterium, and CDI results in the worsening of underlying IBD, increasing the risk of IBD treatment failure, surgery, and hospitalization. Because the symptoms of CDI overlap with those of IBD, it is challenging to make a differential diagnosis. Therefore, early, rapid, and reliable diagnostic tools that can identify CDI in IBD patients would be valuable to clinicians. Methods: This study retrospectively collected 135 patients with IBD. Among them, 44 patients were diagnosed with CDI, and 42 patients were diagnosed with viral or fungal infections. A total of 49 patients without infections were defined as the control group. The diagnostic values of procalcitonin (PCT), C-reactive protein (CRP), and white blood cell (WBC) count in the peripheral blood were examined. Results: In this study, PCT levels were significantly higher in patients with CDI than in non-CDI patients (including patients with viral/fungal infections and the control group; p < 0.001 and p < 0.05, respectively). CRP levels were significantly higher in patients with CDI than in non-CDI patients (p < 0.05). The area under the curve (AUC) of PCT and WBC count were compared using DeLong's test: the AUCs of PCT vs. CRP for the detection of the IBD–CDI group and the control group was 0.826 [95% confidence interval (CI) 0.743–0.909] vs. 0.663 [95% confidence interval (CI) 0.551–0.774] (p < 0.05), respectively. WBC count was inferior as a diagnostic tool for CDI. The sensitivity was 59.09% (95% CI: 43.2% to 73.7%), the specificity was 89.80% (95% CI: 77.8% to 96.6%), and the positive likelihood ratio LR (+) was 5.79 for PCT for the diagnosis of CDI. Conclusions: The present study demonstrates the superiority of PCT over CRP and WBC count for the rapid diagnosis of CDI in IBD patients. [ABSTRACT FROM AUTHOR]

The research into and production of hematological reference samples used to implement an external quality assessment (EQA) to check the quality of hematology tests are necessary for hematology laboratories in Vietnam. In this research, the study team determined the assessment values of blood cell count (human RBCs, pseudo-leucocytes, and pseudo-platelets) by the impedance method used in hematology EQA programs. The hematological reference samples were controlled at three concentration levels: low, normal, and high. Determination of the assigned value (mean ± 2SD) was performed for the following hematology analyzer series by impedance method: ABX Micros 60, Celldyn 1700, and Mindray BC 2000. Each device was sent to 10 different laboratories for evaluation. Research results for assigned values of each model (ABX Micros 60, Celldyn 1700, and Mindray BC 2000) were determined at the three concentrations. For the ABX Micros 60 and Celldyn 1700 series, 80% of laboratories had analytical results within assigned values. For the Mindray BC 2000 series, 100% of laboratories had analytical results within assigned values. The measurement results for the number of human RBCs, pseudo-leucocytes, and pseudo-platelets on each analyzer were similar between the 10 laboratories; the results of the three hematology analyzer series using the impedance method were different and the difference was statistically significant (p < 0.05). Thus, hematological reference samples for measuring the number of blood cells meeting the standards so that they can evaluate the results of laboratories using the impedance method: ABX, Celldyn 1700, Mindray BC 2000. [ABSTRACT FROM AUTHOR]

Study Objectives: Narcolepsy type 1 (NT1) is an autoimmune disease caused by the selective immune attack against orexin-producing neurons. However, the pathophysiology of narcolepsy type 2 (NT2) and idiopathic hypersomnia (IH) remains controversial. The neutrophil-to-lymphocyte ratio (NLR) is an easily calculated parameter from the white blood cell count, which has already been extensively used as an inflammatory marker in immunological disorders. In this study, we examined the white blood cell count of patients with NT1, NT2, and IH compared to healthy controls (HC) and evaluated the NLR to test the possibility of identifying an easy biofluid marker for detecting inflammation and distinguishing patients from HC., Methods: White blood cell count and NLR were compared between 28 patients with NT1, 17 with NT2, 11 with IH, and 21 sex/age-matched HC. These parameters were correlated with cerebrospinal fluid levels of orexin-A, the cerebrospinal fluid/serum albumin ratio (as a marker of blood-brain barrier integrity), and polysomnographic parameters., Results: Patients with NT1 (NLR 2.01 ± 0.44) showed significantly higher NLR than those with NT2 (NLR 1.59 ± 0.53) or IH (NLR 1.48 ± 0.37) and HC (NLR 1.48 ± 0.43). Correlation analysis did not document significant associations between NLR and the other biological markers in each group of patients. The receiver operating characteristic curve analysis detected an optimal cutoff value to discriminate patients with NT1 from those with NT2, IH, and HC for values of NLR ≥ 1.60, 1.62, and 1.59, respectively., Conclusions: Patients with NT1 showed a higher NLR than those with NT2, IH, and HC, possibly reflecting lymphocyte migration within the central nervous system, supporting the hypothesis of a neuroinflammatory attack of lymphocytes against orexin-producing neurons. Considering its sensitivity, this easily obtainable biofluid marker could help to screen patients with NT1., Citation: Conti M, Cirillo F, Maio S, et al. Increased neutrophil-to-lymphocyte ratio as a possible marker to detect neuroinflammation in patients with narcolepsy type 1. J Clin Sleep Med. 2025;21(1):101-107., (© 2025 American Academy of Sleep Medicine.)

Abstract Background Chronic inflammatory diseases are linked to an increased risk of stroke events. The white blood cell (WBC) count is a common marker of the inflammatory response. However, it is unclear whether the WBC count, its subpopulations and their dynamic changes are related to the risk of fatal stroke in relatively healthy elderly population. Methods In total, 27,811 participants without a stroke history at baseline were included and followed up for a mean of 11.5 (standard deviation = 2.3) years. After review of available records, 503 stroke deaths (ischaemic 227, haemorrhagic 172 and unclassified 104) were recorded. Cox proportional hazards regression was used to assess the associations between the WBC count, its subpopulations and their dynamic changes (two-phase examination from baseline to the 1st follow-up) and the risk of fatal all stroke, fatal ischaemic stroke and fatal haemorrhagic stroke. Results (i) Regarding the WBC count in relation to the risk of fatal stroke, restricted cubic splines showed an atypically U-curved association between the WBC count and the risk of fatal all stroke occurrence. Compared with those in the lowest WBC count quartile ( 7.2*10^9/L) had a 53 and 67% increased risk for fatal all stroke (adjusted hazard ratio [aHR] = 1.53, 95% confidence interval (CI) 1.16–2.02, P = 0.003) and fatal haemorrhagic stroke (aHR = 1.67, 95% CI 1.10–2.67, P = 0.03), respectively; compared with those in the lowest quartile ( 4.5*10^9/L) had a 45 and 65% increased risk for fatal all stroke (aHR = 1.45, 95% CI 1.10–1.89, P = 0.008) and fatal ischaemic stroke (aHR = 1.65, 95%CI 1.10–2.47 P = 0.02), respectively. With the additional adjustment for C-reactive protein, the same results as those for all stroke and ischaemic stroke, but not haemorrhagic stroke, were obtained for the WBC count (4 ~ 10*10^9/L) and the NEUT count (the NEUT counts in the top 1% and bottom 1% at baseline were excluded). (ii) Regarding dynamic changes in the WBC count in relation to the risk of fatal stroke, compared with the stable group (− 25% ~ 25%, dynamic changes from two phases of examination (baseline, from September 1st, 2003 to February 28th, 2008; 1st follow-up, from March 31st 2008 to December 31st 2012)), the groups with a 25% increase in the WBC count and NEUT count respectively had a 60% (aHR = 1.60, 95% CI 1.07–2.40, P = 0.02) and 45% (aHR = 1.45, 95% CI1.02–2.05, P = 0.04) increased risk of fatal all stroke occurrence. Conclusions The WBC count, especially the NEUT count, was associated with an increased risk of fatal all stroke occurrence. Longitudinal changes in the WBC count and NEUT count increase in excess of 25% were also associated with an increased risk of fatal all stroke occurrence in the elderly population.

Background: Ventilator-associated pneumonia (VAP) continues to plague patients in intensive care units (ICUs) throughout the world. Persistent leukocytosis despite antibiotic treatment for VAP can have many etiologies including normal inflammatory response, inadequate VAP antimicrobial therapy, and the presence of additional infectious diagnoses. Hypothesis: Surgical patients with VAP and a second infectious source have a different white blood cell count (WBC) trend than patients with VAP alone. Patients and Methods: Retrospective, single-center study of surgical ICU patients diagnosed with VAP (>104 CFU/mL on semi-quantitative culture) between January 2019 and June 2020. Chart review identified additional infections diagnosed during VAP treatment. White blood cell count values were compared between patients treated for VAP alone (VAP-alone) and those with additional infections (VAP-plus) using a Wilcoxon test. Univariable analysis compared admission type, surgeries, and steroid use between cohorts. Results: Eighty-eight VAPs were included for analysis; 61 (69%) were VAP-alone and 27 (31%) VAP-plus. Average age was 47.1 ± 16.7 years, 78% were male, and 93% were trauma admissions. Median hospital day of VAP diagnosis was six (interquartile range [IQR], 4-10). Nearly all patients (99%) were started on initial antibiotic agents to which the VAP organism was sensitive. Daily WBC was higher for VAP-plus compared with VAP-alone on days five, six, and seven of treatment. The maximum WBC was higher for VAP-plus (21.6 k/mcL vs. 16.1 k/mcL; p = 0.02). There were no differences in admission types, number of surgeries, or steroid use between groups. Conclusions: Providers should have increased suspicion for additional sources of infection when ICU patients with a VAP continue to have elevated WBC despite appropriate antibiotic therapy. [ABSTRACT FROM AUTHOR]

Dysferlin (DYSF) has drawn much attention due to its involvement in dysferlinopathy and was reported to affect monocyte functions in recent studies. However, the role of DYSF in the pathogenesis of atherosclerotic cardiovascular diseases (ASCVD) and the regulation mechanism of DYSF expression have not been fully studied. In this study, Gene Expression Omnibus (GEO) database and epigenome-wide association study (EWAS) literatures were searched to find the DNA methylation-driven genes (including DYSF) of ASCVD. The hub genes related to DYSF were also identified through weighted correlation network analysis (WGCNA). Regulation of DYSF expression through its promoter methylation status was verified using peripheral blood leucocytes (PBLs) from ASCVD patients and normal controls, and experiments on THP1 cells and Apoe-/- mice. Similarly, the expressions of DYSF related hub genes, mainly contained SELL, STAT3 and TMX1, were also validated. DYSF functions were then evaluated by phagocytosis, transwell and adhesion assays in DYSF knock-down and overexpressed THP1 cells. The results showed that DYSF promoter hypermethylation up-regulated its expression in clinical samples, THP1 cells and Apoe-/- mice, confirming DYSF as a DNA methylation-driven gene. The combination of DYSF expression and methylation status in PBLs had a considerable prediction value for ASCVD. Besides, DYSF could enhance the phagocytosis, migration and adhesion ability of THP1 cells. Among DYSF related hub genes, SELL was proven to be the downstream target of DYSF by wet experiments. In conclusion, DYSF promoter hypermethylation upregulated its expression and promoted monocytes activation, which further participated in the pathogenesis of ASCVD. [ABSTRACT FROM AUTHOR]

Introduction: Periprosthetic joint infections (PJI) following primary arthroplasty continue to be a serious complication, despite advances in diagnostics and treatment. Two-stage revision arthroplasty has been commonly used as the gold standard for the treatment of PJI. However, much discussion persists regarding the interim of the two-stage procedure and the optimal timing of reimplantation. Serology markers have been proposed as defining parameters for a successful reimplantation. The objective of this matched-pair analysis was to assess the role of serum C-reactive protein (CRP) and white blood cell count (WBC) in determining infection eradication and proper timing of reimplantation. We investigated the delta (∆) change in CRP and WBC values prior to both stages of two-stage revision arthroplasty as a useful marker of infection eradication. Methods: We analyzed 39 patients and 39 controls, matched by propensity score matching (BMI, age, ASA-classification), with a minimum follow-up of 24 months and treated with a two-stage revision THA or TKA in our institution. Data of serum CRP and WBC values were gathered at two selected time points: prior to the explantation of the implant (preexplantation) and following the completion of antibiotic treatment regimen, both systemic and with a drug-eluting cement spacer (prereimplantation). Patient records were reviewed electronically for preexisting comorbidities, overall health status, synovial fluid cultures, inflammatory serologies, revision surgeries, and recurrent or persistent infection based on the modified Musculoskeletal Infection Society criteria. Patient demographics, ∆CRP, ∆WBC, and time interval to reimplantation were statistically analyzed using receiver operator curves (ROC), Pearson's correlation coefficient, Levene's test, and Student's t-test. Results: Infection-free patients exhibited higher mean CRP and WBC than did patients who were reinfected at both time points. When comparing preexplantation with prereimplantation values, the median ∆CRP was 9.48 mg/L (interquartile range (IQR) 2.3–36.6 mg/L) for patients who did not develop a reinfection versus 2.74 mg/L (IQR 1.4–14.2 mg/L) for patients who developed reinfection (p = 0.069). The median ∆WBC was 1.5 × 109/L (IQR 0.6–4.0 × 109/L) for patients who remained infection-free versus 1.2 × 109/L (IQR 0.8–2.2 109/L) for patients who developed reinfection (p = 0.072). Analysis of areas under the curve (AUC) using ROC demonstrated poor prediction of persistent infection by ∆CRP (AUC = 0.654) and ∆WBC (AUC = 0.573). Although a highly significant correlation was found between the interim interval and infection persistence (r = 0.655, p < 0.01), analysis using ROC failed to result in a specific threshold time to reimplantation above which patients are at significantly higher risk for reinfection (AUC = 0.507). Conclusion: No association could be determined between the delta change in serum CRP and WBC before and after two-stage revision arthroplasty for PJI and reinfection risk. Even though inflammatory serologies demonstrate a downtrending pattern prior to reimplantation, the role of CRP and WBC in determining the optimal timing of reimplantation seems to be dispensable. Planning a second-stage reimplantation requires assessing multiple variables rather than relying on specific numeric changes in these inflammatory marker values. [ABSTRACT FROM AUTHOR]

This study was done to investigate the effects of ultraviolet radiation—(UVA) on blood indices of albino rats after continuous exposure to some doses of UVA. The mole-rats of 180–200 g body weight were divided into the control and radiation-trial groups. The control group was not given any radiation. The other groups were irradiated with artificially produced UVA radiation for 17, 27, and 59 days. In addition, Radiation has a wide variety of effects on the liver. So this study focuses on the effects of various doses of radiation in the liver tissues of rats. Inductively coupled plasma-mass spectrometry (ICP-MS) was used to measure levels of trace elements in the liver tissue of rats. The result showed the concentration of Mg, Cu, K, and Fe changed compared with control, and the liver can repair changes and return to the normal range. But Ca has a significant increase in liver tissue, unlike Na, Zn, and Cr have significant. [ABSTRACT FROM AUTHOR]

Rekayasa ekologi melalui manipulasi habitat merupakan bentuk konservasi ekologi yang bertujuan melestarikan keberadaan musuh alami, salah satunya golongan predator generalis. Usaha ini merupakan strategi PHT untuk menangani masalah WBC (Nilaparvata lugens). Penelitian ini bertujuan untuk mengamati perkembangan populasi WBC dan predator generalis pasca penerapan rekayasa ekosistem. Penelitian dilakukan di lahan persawahan Desa Tejoasri, Laren, Lamongan dengan mengamati populasi WBC, predator umum, pertumbuhan dan perkembangan tanaman, dan analisis usahatani. Â Plot PHT berbasis rekayasa ekosistem (PHT-RE), PHT konvensional (PHT), dan budidaya konvensional digunakan untuk memperoleh data. Hasil rerata populasi WBC pada perlakuan PHT-RE (8,73 ekor) , berbeda sedikit dengan hasil plot PHT (8,55 ekor), namun hasil keduanya jauh lebih rendah dibandingkan dengan sistem konvensional (14,27 ekor). Predator generalis yang ditemukan pada saat pengamatan terdiri dari laba-laba, capung, kumbang kubah (Coccinelidae), kumbang botol (Ophionea indica), dan Paederus sp. Jenis predator spesialis juga ditemukan yaitu Cyrtorhinus lividipennis. Populasi predator generalis tertinggi ditemukan pada petak PHT-RE (1069 ekor), diikuti petak PHT (656 ekor) dan konvensional (426 ekor). Nilai BCR yang diperoleh pada perlakuan PHT-RE, PHT, dan konvensional berturut-turut yaitu 3,78, 3,25, dan 1,84.

White blood cells (WBCs) are the important constituent of a blood cell. These blood cells are responsible for defending the body against infections. Abnormalities identified in WBC smears lead to the diagnosis of disease types such as leukocytosis, hepatitis, and immune system disorders. Digital image analysis for infection detection at an early stage can help fast and precise diagnosis, as compared to manual inspection. Sometimes, acquired blood cell smear images from an L2-type microscope are of very low quality. The manual handling, haziness, and dark areas of the image become problematic for an efficient and accurate diagnosis. Therefore, WBC image enhancement needs attention for an effective diagnosis of the disease. This paper proposed a novel virtual hexagonal trellis (VHT)-based image filtering method for WBC image enhancement and contrast adjustment. In this method, a filter named the virtual hexagonal filter (VHF), of size 3 × 3, and based on a hexagonal structure, is formulated by using the concept of the interpolation of real and square grid pixels. This filter is convolved with WBC ALL-IBD images for enhancement and contrast adjustment. The proposed filter improves the results both visually and statically. A comparison with existing image enhancement approaches proves the validity of the proposed work. [ABSTRACT FROM AUTHOR]

Objective: Comparative effect of Curcuma longa (C. longa) ethanolic extract and curcumin on paraquat (PQ)-induced systemic and lung oxidative stress and inflammation were evaluated in the present study. Materials and Methods: Control animals were exposed to normal saline and PQ group to 54 mg/m3 PQ aerosols (8 times, each time for 30 min). Treatment groups were exposed to PQ and treated with 150 and 600 mg/kg/day C. longa, or 30 and 120 mg/kg/day curcumin after PQ exposure period for 16 days. Total and differential white blood cells (WBC) and oxidative markers were measured both in bronchoalveolar lavage (BALF) and blood at the end of the study. Results: Total and differential WBC counts as well as malondialdehyde (MDA) level were significantly increased but total thiol content and the activities of catalase (CAT) and superoxide dismutase (SOD) were reduced in both the BALF and blood of the PQ group in comparison with the control group (p<0.05 to p<0.001). Both doses of C. longa and curcumin diminished MDA level, total and differential WBC counts in the blood and BALF but increased CAT and SOD activities in both of them compared to PQ group (p<0.05 to p<0.001). The effects of C. longa and curcumin high dose on most variables were markedly more than low dose (p<0.05 to p<0.001). Furthermore, the effects of curcumin on some variables were markedly more than C. longa (p<0.05 to p<0.001). Conclusion: Both C. longa and curcumin improved PQ-induced systemic and lung inflammation and oxidative stress, but the effect of curcumin was more prominent. [ABSTRACT FROM AUTHOR]

Background/Aim: Convalescent plasma (CP) therapy, which includes processing and administering antibody-rich plasma from recovered patients to sick patients, is used for passive immunity in COVID-19 pneumonia patients in addition to antivirals and antibiotics. This study aimed to assess whether CP treatment significantly affects hyperviscosity and COVID-19 prognosis-related blood parameters. Methods: This study was a single-center retrospective cohort study. Ninety-seven patients with COVID-19 polymerase chain reaction (PCR)-positive results and pneumonia observed on thoracic computed tomography (CT) were included. Patients' ferritin, d-dimer, C-reactive protein (CRP), and complete blood count levels before and after CP administration were compared. Results: Ferritin, d-dimer, white blood cell (WBC), neutrophil, and plateletcrit (PCT) levels and the platelet distribution width (PDW) were significantly higher and there was a significant decrease in the CRP level after CP treatment compared to before CP (P < 0.05). Ferritin, d-dimer, and CRP values measured after CP were higher in deceased patients than in survivors (P = 0.001, P = 0.007, and P < 0.001, respectively). Conclusion: Ferritin, d-dimer, WBC, and neutrophil levels, which we expected to decrease on the basis of the COVID-19 prognosis, unfortunately increased, and only CRP levels decreased. However, we found that these increases were more pronounced in patients who died. Considering these prognostic factors, the findings of our study suggest that CP treatment has no effect on the COVID-19 disease course and may lead to a worse prognosis. [ABSTRACT FROM AUTHOR]

Background: The development of tools that could help emergency department clinicians recognize stroke during triage could reduce treatment delays and improve patient outcomes. Growing evidence suggests that stroke is associated with several changes in circulating cell counts. The aim of this study was to determine whether machine-learning can be used to identify stroke in the emergency department using data available from a routine complete blood count with differential.Methods: Red blood cell, platelet, neutrophil, lymphocyte, monocyte, eosinophil, and basophil counts were assessed in admission blood samples collected from 160 stroke patients and 116 stroke mimics recruited from three geographically distinct clinical sites, and an ensemble artificial neural network model was developed and tested for its ability to discriminate between groups.Results: Several modest but statistically significant differences were observed in cell counts between stroke patients and stroke mimics. The counts of no single cell population alone were adequate to discriminate between groups with high levels of accuracy; however, combined classification using the neural network model resulted in a dramatic and statistically significant improvement in diagnostic performance according to receiver-operating characteristic analysis. Furthermore, the neural network model displayed superior performance as a triage decision making tool compared to symptom-based tools such as the Cincinnati Prehospital Stroke Scale (CPSS) and the National Institutes of Health Stroke Scale (NIHSS) when assessed using decision curve analysis.Conclusions: Our results suggest that algorithmic analysis of commonly collected hematology data using machine-learning could potentially be used to help emergency department clinicians make better-informed triage decisions in situations where advanced imaging techniques or neurological expertise are not immediately available, or even to electronically flag patients in which stroke should be considered as a diagnosis as part of an automated stroke alert system. [ABSTRACT FROM AUTHOR]

This paper describes the study of overlapping leukaemia cells based on geometric features for identification and classification. Geometric features of blood cells are proposed to identify and classify overlapping cells into groups based on different overlapping degrees and the number of overlapped cells. In the proposed method, the percentage of average accuracy for identifying overlapping cells reached 98 percent. The proposed method can segment white blood cells from the overlapping cells with an overlapping degree of 70 percent. Improved Watershed Algorithm successfully increased 36.89 percent of accuracy in WBC segmentation. It reduced 46.12 percent of the over-segmentation problem. Tests of cell counting are conducted in the two stages, which are before and after the process of identification and classification of overlapping cells. The average percentage of total cell count is 83.31 percent, the average percentage of WBC counting is 84.8 percent, and the average percentage of RBC counting is 60.55 percent. The proposed method is efficient in identifying and classifying overlapping cells for increasing the accuracy of cell counting. [ABSTRACT FROM AUTHOR]

Low patient motivation and relatively long procedures can sometimes limit the gains of the PAP test. At this point, the clinician may need additional stimulating tests to persuade patients to perform screening tests. The aim of this study was to determine the predictive value of hematological parameters for the high-grade squamous intraepithelial lesion (HG-SIL). Ninety-one patients possessing HG-SIL with complete blood counts and 111 healthy patients with normal cervical cytology were reviewed retrospectively. The age, WBC, and RBC count were significantly different in patients with HG-SIL than in controls. A cutoff value was found 42.5 for age (42% sensitivity and 58 % specificity, AUC: 0.604), 8.65 for WBC (44% sensitivity and 68% specificity, AUC: 0.570), and 4.47 for RBC (65% sensitivity and 58% specificity, AUC: 0.628) in ROC curve analysis. The results of the study show that the age of the patients, RBC, and WBC counts are important in precancerous intraepithelial lesions. Clinicians should warn female patients in the risk group to have regular gynecological examinations and screenings. [ABSTRACT FROM AUTHOR]

Introduction: Differential counts of leukocytes are frequent, and often several automated blood cell counters are needed in contemporary laboratories. However, these modules are often individually quality assured. Our aim was therefore to validate the interchangeability of five hematology modules in a large modern laboratory and to compare them with our gold standard (GS) manual white blood cell differential count. Methods: At Copenhagen University Hospital, we compared five Sysmex XN‐modules for neutrophils, lymphocytes, monocytes, eosinophils, basophils, and immature granulocytes (IG). We analyzed control samples in three levels to evaluate intra‐ and intermodular precision. Bias between modules was evaluated by analyzing 93 random patient samples within reference intervals. XN‐modules' mean counts were compared with GS. Results: We found acceptable intramodular CV% (0.92%–8.76%), only neutrophils and eosinophils exceeded state‐of‐the‐art imprecision or desirable specifications for medium control levels. Intermodular CV% showed significance difference for only monocytes (ANOVA, P <.0001). For patient samples, there were significant differences between XN‐modules regarding four WBC types (ANOVA); however, proportional bias ranged from 1.7% to 3.8%, being within desirable specifications except basophils and IG (bias = 13.3% and 24.9%, respectively). Comparisons with GS, XN‐modules exceeded desirable bias for basophils (lower than GS); monocytes and IG (higher than GS). Conclusion: This multimodule comparison shows acceptable intermodular imprecision and bias for clinical purposes, which is important for patient safety. Similar multimodule study should be performed with samples out of reference range in large‐scale laboratories to confirm the interchangeability. [ABSTRACT FROM AUTHOR]

Environmental factors such as heat stress have a considerable impact on animal productivity, health, and well-being. Our objectives were to measure the physiological response to heat stress in Karadi cows, and to measure the production level of those cows. Sixteen multiparous Karadi cows were randomly assigned to 1 of 2 treatments: heat stress (HT, n = 8) or cooling (CL, n = 8), depending on the yield of mature equivalent milk (HT: 1.72 ± 0.23 kg; CL: 1.83 ± 0.23 kg) of the daily lactating. The average HT and CL cow parity were 3.5 ± 0.4 for each, and the average body weight of HT and CL cows was 197 ± 5.6 kg and 200 ± 5.6 kg, respectively. Rectal temperature (RT), skin temperature (ST), and respiration rate (RR) were recorded once daily (1400 h). Dry matter intake, water intake, milk yield, and milk composition were recorded daily, as well. Blood sample was collected three times; the beginning, the mid, and the end of the study for white blood cell (WBC) count. Cows from the HT treatment possessed a higher rectal temperature, skin temperature, and respiration rate in comparison to the cooling system. Compared with CL cows, HT cows produced less milk, solids-nonfat (SNF), protein, and lactose. However, no treatment effect was observed for concentrations of milk fat. Relative to CL cows, HT cows consumed less dry matter intake and consumed more water intake. Regarding WBC count, HT cows tended to have more WBC cells compare to CL cows. Additionally, HT cows have a greater number of neutrophils and monocytes compared to CL cows. No effect of treatment was observed for number and percentage of lymphocytes, percentage of neutrophils, and percentage of monocytes. Thus, heat stress negatively affects the milk production and immunity of Karadi cows. [ABSTRACT FROM AUTHOR]

Introduction. Multiple sclerosis (MS) is one of the main chronic inflammatory diseases of the central nervous system that causes functional disability in people. Assessment of the state of the immune system begins with an analysis of the total number of blood leukocytes and their population composition (the number of neutrophils and lymphocytes). The ratio of neutrophils and lymphocytes (NRL) is a marker of inflammation.The aim of the study. The aim of this study was to investigate the WBC and neutrophil-to-lymphocyte ratio in patients with different forms of MS. Results. In patients with MS in 23.5% of cases the WBC is higher than 9×109/L. In 5.9% of МS cases leukopenia is observed, and in the vast majority of cases (70, 6%) the level of blood leukocytes was in the range of 4-9×109/L. Leukopenia and leukocytosis are more common in women than in men (pConclusions. NRL is an inexpensive and affordable indicator, which is determined by the automatic determination of the total number of leukocytes in the blood. It can be an additional informative indicator of inflammation against the background of normal leukocyte levels.

INTRODUCTION: We aimed to evaluate the frequency of reproduction in urine culture and to evaluate the most common microorganism and the relationship between the findings and acute phase markers in patients with urinary tract infection. METHODS: Patients aged 0-18 years (n=64420) who applied to the pediatric emergency department were retrieved from computer records between January 2018 and December 2018. A total of 1137 patients with fever of 38.0 ℃ and above who had records of urine culture, CRP, platelet, WBC tests were included into the study. RESULTS: E.coli was the most common bacteria in urine culture (18.4%). The mean age (5.09 +- 4.58) of the patients with culture reproduction was lower (p

The influence of antibiotic enrofloxacin and PEG-400 polymer complex on hemoglobin content, red and white blood cell count, and blood leukogram state of apparently healthy rats has been studied. The enrofloxacin contains reactive carboxyl groups in the molecule structure, it makes possible to carry out modifications for obtaining new compounds. The complex of enrofloxacin with polymer was obtained by interaction of enrofloxacin hydrochloride with polyethylene glycol-400. There were not any abnormal changes in the physiological state of the animals after administration none of the tested substances. The effect of the enrofloxacin + PEG400 complex in 7 days after the injection was manifested by the decrease of red blood cells count and hemoglobin concentration in the rats’ blood, and after 14 and 21 days — by activation of hematopoietic function. Particularly, on the 14th day red blood cell counts in the blood of control animals and animals treated with enrofloxacin+PEG-400 complex were approximately equal and ranged 4.6–4.7×1012/L, and were 8.0–16.4% (P>0.05) lower than in experimental rats received the antibiotic and PEG-400 separately. At the same time, in the absence of significant difference in red blood cell count, the blood hemoglobin concentration increased. This concentration reached a maximum after the administration of the enrofloxacin + PEG-400 complex (132.2±4.10 g/L), but it became a little bit lower (2.7–8.9 g/L) in rats’ blood of other experimental groups. The effects of PEG-400 and enrofloxacin both separately and in complex of enrofloxacin + PEG400, were characterized by the decrease in leukocytes count throughout the experiment. So, after the 21st day of experiment white blood cell count was the lowest in the blood of animals after the enrofloxacin + PEG-400 complex administration (4.2±0.41×109/L; P

Purpose: The aim of this study was to analyze the predictive potential of selected biochemical and hematological parameters in patients with gout from Bosnia and Herzegovina, and to determine the most common comorbidities related to gout. Methods: Forty patients with gout (17 males and 23 females) from the hospital in the municipality of Novi Grad, Sarajevo were included in the present study. We analyzed biochemical parameters (uric acid, urea, creatinine, and CRP), ESR, and WBC as a hematological parameter. The Shapiro-Wilk test and the Spearman correlation coefficient were used for the normality and correlation analysis, respectively. Results: Most gout patients were 50-70 years old and gout was more prevalent in female patients. Thirteen different comorbidities are related to gout. After the onset of the main disease, 82.35% of male patients were diagnosed with cardiovascular disease, while hypertension was diagnosed in 76.47% of male patients. High mean values of ESR, as well as CRP, uric acid, urea, and creatinine concentrations were found for all gout patients. A strong positive correlation was found after comparing urea and ESR (ρ=0.314); urea and creatinine (ρ=0.526); CRP and ESR (ρ=0.375). Conclusion: We can conclude that the analyzed biochemical parameters, CRP, urea, creatinine, and uric acid, as well as ESR, are good predictors of gout. High values of uric acid, creatinine, and urea are the most important gout predictors and significant markers for the development of comorbidities.

During the COVID-19 pandemic, different SARS-CoV-2 variants of concern (VOC) with specific characteristics have emerged and spread worldwide. At the same time, clinicians routinely evaluate the results of certain blood tests upon patient admission as well as during hospitalization to assess disease severity and the overall patient status. In the present study, we searched for significant cell blood count and biomarker differences among patients affected with the Alpha, Delta and Omicron VOCs at admission. Data from 330 patients were retrieved regarding age, gender, VOC, cell blood count results (WBC, Neut%, Lymph%, Ig%, PLT), common biomarkers (D-dimers, urea, creatinine, SGOT, SGPT, CRP, IL-6, suPAR), ICU admission and death. Statistical analyses were performed using ANOVA, the Kruskal–Wallis test, two-way ANOVA, Chi-square, T-test, the Mann–Whitney test and logistic regression was performed where appropriate using SPSS v.28 and STATA 14. Age and VOC were significantly associated with hospitalization, whereas significant differences among VOC groups were found for WBC, PLT, Neut%, IL-6, creatinine, CRP, D-dimers and suPAR. Our analyses showed that throughout the current pandemic, not only the SARS-CoV-2 VOCs but also the laboratory parameters that are used to evaluate the patient’s status at admission are subject to changes.

Anastomotic leaks (ALs) remain a serious postoperative complication in colorectal surgery, often resulting in significant morbidity, prolonged hospitalization, and increased mortality risk. This systematic review aims to evaluate the role of predictive biomarkers in the early detection of ALs, focusing on their diagnostic accuracy and clinical utility. Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a comprehensive literature search was conducted across MEDLINE, Scopus, CENTRAL, and Web of Science, identifying studies that examined biomarkers such as C-reactive protein (CRP), procalcitonin (PCT), and white blood cell (WBC) count in the context of AL. A total of 20 studies met the inclusion criteria, with sample sizes ranging from 59 to 2,655 patients undergoing colorectal surgeries with primary anastomosis. CRP emerged as the most widely studied and reliable biomarker, with studies suggesting that elevated CRP levels, particularly on postoperative days 3-4, can effectively indicate AL risk, showing high negative predictive value. PCT has also shown promise as a complementary biomarker, offering enhanced specificity for infectious complications. Although WBC count alone was a limited predictor, it may add diagnostic value when used with other markers. In addition, innovative biomarkers, such as inflammatory indices in peritoneal fluid, demonstrated potential for further improving AL detection accuracy., Competing Interests: Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2024, Dost et al.)