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In recent years, microwave-assisted extraction (MAE) has attracted considerable interest to extract valuable compounds from unutilized or underutilized sources such as radish leaves with great extraction efficiency. Herein, the continuous fast MAE of radish leaves polysaccharides (RLPs) was investigated and optimized in detail. The maximum predicted extraction yield (EY) of RLPs was found to be 25.51% under the optimum MAE conditions as microwave power of 104.65 W, irradiation time 7.59 min, and pH 3.24. The MAE resulted in notably higher EY than hot water extraction (HWE) at pH of 7.50, temperature of 85 °C, liquid–solid ratio of 30:1 mL g−1 for 180 min. Polysaccharides characteristic bands in RLPs were detected in the FT-IR spectrum. The RLPs was mainly composed of galactose (43.4%), arabinose (24.7%), galacturonic acid (6.8%), rhamnose (9.3%), mannose (5.7%), fucose (5.0%), glucose (3.9%), glucuronic acid (0.7%), and xylose (0.5%). Moreover, the RLPs showed strong scavenging potential on DPPH free radicals, reducing power, and lipid peroxidation inhibition in a concentration-dependent mode. The RLPs exhibited good water solubility, water-holding, and fat-binding capacities, recorded at 59.36 ± 0.58%, 4.25 ± 0.09, and 5.41 ± 0.009 g g−1, respectively and showed good emulsion properties even after 168 h (74.5 ± 0.04%). It is clear from the results that radish leaves are valuable source of polysaccharides and RLPs can be a promising natural candidate agent in the food and medicinal industries. Moreover, these findings proved that MAE is a highly efficient and promising procedure to extract RLPs to the maximum extent. [ABSTRACT FROM AUTHOR]

The aim of the study was to determine the effect of ozone gas fumigation on the mechanical, chemical, and microbiological parameters of radish tubers. Radish plants were grown in the ground in accordance with the principles of good agricultural practice and condition suitable for the soil and climatic conditions of south-eastern Poland. At the end of the growing season, 24 h before harvest, radish plants were exposed to a variable factor, i.e., fumigation with ozone gas at various doses (1 ppm for 1 and 5 min; 5 ppm for 1 and 5 min) in order to modify selected metabolic pathways of bioactive compounds. Then, 24 h after ozonation, radish tubers were harvested and placed in a climatic chamber with controlled conditions, i.e., 2 °C and 90% humidity. Laboratory analyses were performed during storage on days 1, 5, and 10. The ozonation used did not significantly improve the mechanical properties and water content of radish tubers. There was a beneficial effect of selected gaseous ozone doses (1 ppm for 1 and 5 min; 5 ppm for 1 min on the 10th day of storage) on the biosynthesis of selected bioactive compounds, i.e., ascorbic acid content, total polyphenol content, and antioxidant potential during storage. The most beneficial effects of the use of gaseous ozone were observed in the storage process in reducing the microbiological load of radish tubers. Among the ozonation doses used, the dose of 5 ppm for 5 min had the most beneficial effect on reducing the microbiological load. It reduced the number of yeasts and molds by 14.2% and aerobic mesophilic bacteria by 20.9% compared to the control sample on the last day of storage. Additionally, between the 5th and 10th day of storage, a significant effect of each ozone dose applied on reducing the occurrence of yeasts, molds, and mesophilic aerobic bacteria during tuber storage was noted. [ABSTRACT FROM AUTHOR]

The study aimed to enhance quercetin production in radish by optimizing Agrobacterium tumefaciens-mediated in-planta transformation. This protocol involved infecting radish seed embryo axis with A. tumefaciens EHA105 strain carrying the 35S::AtMYB12. Radish seeds were infected with the Agrobacterium suspension (0.8 OD600) for 30 min, followed by sonication for 60 s and vacuum infiltration for 90 s at 100 mm Hg. A 3-day co-cultivation in Murashige and Skoog medium with 150 μM acetosyringone yielded a transformation efficiency of 59.6% and a transgenic callus induction rate of 32.3%. Transgenic plant and callus lines were confirmed by GUS histochemical assay, PCR, and qRT-PCR. The transgenic lines showed an increased expression of flavonoid pathway genes (AtMYB12, CHS, F3H, and FLS) and antioxidant genes (GPX, APX, CAT, and SOD) compared to WT plants. Overexpression of AtMYB12 in transgenic callus increased enzyme activity of phenylalanine ammonia lyase, catalase, and ascorbate peroxidase. In half-strength MS medium with 116.8 mM sucrose, the highest growth index (7.63) was achieved after 20 days. In AtMYB12 overexpressed callus lines, phenolic content (357.31 mg g−1 dry weight), flavonoid content (463 mg g−1 dry weight), and quercetin content (48.24 mg g−1 dry weight) increased significantly by 9.41-fold. Micro-wounding, sonication, and vacuum infiltration improved in-planta transformation in radishes. These high-quercetin-content transgenic callus lines hold promise as valuable sources of flavonoids.

During 21st century, abiotic stress has adversely affected the agriculture crop production around the globe. Keeping in view the food requirement under water shortage condition, a study was planned to investigate the effect of thiamine application on radish crop under drought stress conditions on plant. For study purpose, two varieties of locally available radish (‘Early-Milo’ and ‘Laal-Pari’) were grown with normal water application as well as thiamine (100 mg L-1) application while maintaining a stress condition (60% field capacity). Increasing water deficit stress linearly reduced plant growth, yield and biomass in both varieties by reducing water use efficiency, while significantly enhanced these attributes with thiamine application. Thiamine application under drought stress exerted significant impacts on physiological attributes in both varieties, including enhanced osmolytic attribute in drought conditions and improvements in superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), H2O2, and malondialdehyde (MDA) activities in plant leaves. Antioxidant and osmoprotectant upregulation positively linked to radish crop's drought tolerance. Moreover, PCA and heatmap analysis revealed a significant interdependence among various traits and interconnected in determining the crop's capacity to sustain growth under conditions of drought stress. In crux, thiamine application conclusively enhances radish growth, yield, biomass, physio-chemical and osmolytic attributes, ionic composition and enzymatic antioxidant potential. Therefore, it is recommended to consider the application of thiamine in commercial agriculture practices to mitigate the negative effects of drought stress on radish crop production.

Vegetables are important agricultural products with numerous health benefits. Excessive chemical fertilization to meet the food challenge has resulted in environmental and soil health hazards. Considering this aspect, the current study was conducted with the aim to introduce biostimulants as an alternative to chemical fertilizers to improve soil quality, crop quality, and yield. In the first experiment, the response of radish was noted against the application of glycine (GLY), aspartic acid (ASP), lysine (LYS), vitamin B complex (VBC), and chemical fertilizers (CF). The biostimulants were sourced from Sigma Aldrich and Martin Dow Market Ltd. The results indicated that ASP has significantly improved the phenolic contents in shoot (1.01%) and root (12.23%) compared with CF. Additionally, total protein was significantly increased in shoot with GLY (251.81%) and in root with ASP (57.06%). Shoot ascorbic acid contents were significantly improved with ASP (179.90%), VBC (159.91%), and LYS (139.92%). Plant fresh and dry weight was improved with VBC (478.31%) and ASP (364.73%). The N and P concentrations in radish root were higher in VBC (25.93%) and LYS (100%). Soil organic matter was improved ASP (61.51%), followed by VBC (60.13%). Soil available P concentration was also enhanced with LYS (40.43%), ASP (31.20%), and VBC (23.19%). The second experiment was focused on identifying the response of turnip crop against the following treatments: chemical fertilizers (CF), Isabion® (ISA), 25% CF + LYS + GLY (CLG), 25% CF + ASP + GLY (CAG), and 25% CF + ASP + LYS (CAL). The biostimulants were sourced from Sigma Aldrich Syngenta, Pakistan. The results denoted that CAL and ISA significantly improved the phenolic contents in turnip shoot and root. The ascorbic acid in turnip shoot was improved with CAL (19.27%), CAG (18.13%), ISA (17.68%), and in root with CLG (26.96%). The P concentration in turnip shoot was significantly higher in ISA (19.25%), CLG (16.85%), and CAG (12.26%). Soil total N was improved in all treatments. ISA improved the available P concentration, whereas CF (67.87 mg kg−1) followed by ISA (65.93 mg kg−1) improved the soil available K. Both studies conclude that biostimulants capable of improving vegetable quality.

Radish (Raphanus sativus L.) is a vegetable crop with economic value and ecological significance in the genus Radish, family Brassicaceae. In recent years, developed countries have attached great importance to the collection and conservation of radish germplasm resources and their research and utilization, but the lack of population genetic information and molecular markers has hindered the development of the genetic breeding of radish. In this study, we integrated the radish genomic data published in databases for the development of single-nucleotide polymorphism (SNP) markers, and obtained a dataset of 308 high-quality SNPs under strict selection criteria. With the support of Kompetitive Allele-Specific PCR (KASP) technology, we screened a set of 32 candidate core SNP marker sets to analyse the genetic diversity of the collected 356 radish varieties. The results showed that the mean values of polymorphism information content (PIC), minor allele frequency (MAF), gene diversity and heterozygosity of the 32 candidate core SNP markers were 0.32, 0.30, 0.40 and 0.25, respectively. Population structural analysis, principal component analysis and genetic evolutionary tree analysis indicated that the 356 radish materials were best classified into two taxa, and that the two taxa of the material were closely genetically exchanged. Finally, on the basis of 32 candidate core SNP markers we calculated 15 core markers using a computer algorithm to construct a fingerprint map of 356 radish varieties. Furthermore, we constructed a core germplasm population consisting of 71 radish materials using 32 candidate core markers. In this study, we developed SNP markers for radish cultivar identification and genetic diversity analysis, and constructed DNA fingerprints, providing a basis for the identification of radish germplasm resources and molecular marker-assisted breeding as well as genetic research.

Abstract Background Single nucleotide polymorphisms (SNPs) and insertions/deletions (InDels) are the most abundant genetic variations and widely distribute across the genomes in plant. Development of SNP and InDel markers is a valuable tool for genetics and genomic research in radish (Raphanus sativus L.). Results In this study, a total of 366,679 single nucleotide polymorphisms (SNPs) and 97,973 insertion-deletion (InDel) markers were identified based on genome resequencing between ‘YZH’ and ‘XHT’. In all, 53,343 SNPs and 4,257 InDels were detected in two cultivars by transcriptome sequencing. Among the InDel variations, 85 genomic and 15 transcriptomic InDels were newly developed and validated PCR. The 100 polymorphic InDels markers generated 207 alleles among 200 Chinese radish germplasm, with an average 2.07 of the number of alleles (Na) and with an average 0.33 of the polymorphism information content (PIC). Population structure and phylogenetic relationship revealed that the radish cultivars from northern China were clustered together and the southwest China cultivars were clustered together. RNA-Seq analysis revealed that 11,003 differentially expressed genes (DEGs) were identified between the two cultivars, of which 5,020 were upregulated and 5,983 were downregulated. In total, 145 flowering time-related DGEs were detected, most of which were involved in flowering time integrator, circadian clock/photoperiod autonomous, and vernalization pathways. In flowering time-related DGEs region, 150 transcriptomic SNPs and 9 InDels were obtained. Conclusions The large amount of SNPs and InDels identified in this study will provide a valuable marker resource for radish genetic and genomic studies. The SNPs and InDels within flowering time-related DGEs provide fundamental insight into for dissecting molecular mechanism of bolting and flowering in radish.

Radish is an important root vegetable that is widely grown in Asia. The root-knot nematode (RKN) Meloidogyne incognita seriously affects the growth and development of radish root and causes poor appearance quality. However, the molecular mechanism of radish response to RKNs remains poorly understood. In this study, a total of 220 lncRNAs, 1144 mRNAs, 20 miRNAs and 153 proteins were differential expressed between the RKN-infected and WT samples. Correlation analysis of all DEPs compared with all DGEs showed that 8 mRNAs-DEPs showed a changed abundance. The results showed that 18 DEmiRNAs have 167 target DEGs in 220 miRNA-target modules and 29 DElncRNAs were predicted as putative targets of 16 DEmiRNAs in 37 miRNA-target modules. In all, 6 DGEs in the ABA pathway and 2 DGEs in the JA pathway were identified under RKN infection, respectively. The four regulatory networks of lncRNA-miRNA-mRNA were constructed in response to RKN infection. qRT-PCR analysis found that the expression pattern of 6 DElncRNAs, 6 DEmRNAs, 6 DEmiRNAs and 6 DEPs were consistent with sequencing results. These results provide a theoretical basis for studying the molecular mechanism of radish in response to M. incognita and breeding resistant varieties to this nematode.

In the cosmetics industry, the extract from Raphanus sativus L. is fermented using specific starter cultures. These cosmetic ingredients act as preservatives and skin conditioners. Kombucha is traditionally made by fermenting sweetened tea using symbiotic cultures of bacteria and yeast and is used in cosmetic products. The aim of this study was to evaluate the cosmetic properties of radish leaf and root extract fermented with the SCOBY. Both unfermented water extracts and extracts after 7, 14, and 21 days of fermentation were evaluated. The analysis of secondary plant metabolites by UPLC-MS showed higher values for ferments than for extracts. A similar relationship was noted when examining the antioxidant properties using DPPH and ABTS radicals and the protective effect against H2O2-induced oxidative stress in fibroblasts and keratinocytes using the fluorogenic dye H2DCFDA. The results also showed no cytotoxicity to skin cells using Alamar Blue and Neutral Red tests. The ability of the samples to inhibit IL-1β and COX-2 activity in LPS-treated fibroblasts was also demonstrated using ELISA assays. The influence of extracts and ferments on bacterial strains involved in inflammatory processes of skin diseases was also assessed. Additionally, application tests were carried out, which showed a positive effect of extracts and ferments on TEWL and skin hydration using a TEWAmeter and corneometer probe. The results obtained depended on the concentration used and the fermentation time.

Pithiness is one of the physiological diseases of radishes, which is accompanied by the accumulation of reactive oxygen species (ROS) during the sponging of parenchyma tissue in the fleshy roots. A respiratory burst oxidase homolog (Rboh, also known as NADPH oxidase) is a key enzyme that catalyzes the production of ROS in plants. To understand the role of Rboh genes in radish pithiness, herein, 10 RsRboh gene families were identified in the genome of Raphanus sativus using Blastp and Hmmer searching methods and were subjected to basic functional analyses such as phylogenetic tree construction, chromosomal localization, conserved structural domain analysis, and promoter element prediction. The expression profiles of RsRbohs in five stages (Pithiness grade = 0, 1, 2, 3, 4, respectively) of radish pithiness were analyzed. The results showed that 10 RsRbohs expressed different levels during the development of radish pithiness. Except for RsRbohB and RsRbohE, the expression of other members increased and reached the peak at the P2 (Pithiness grade = 2) stage, among which RsRbohD1 showed the highest transcripts. Then, the expression of 40 genes related to RsRbohD1 and pithiness were analyzed. These results can provide a theoretical basis for improving pithiness tolerance in radishes.

Radish (Raphanus sativus L.) is a member of the Brassicaceae family of root vegetables and both the roots and the leaves are consumed for their natural therapeutic value and health promoting effects owing to the presence of phytochemicals. Thus, the effect of three different solvents (Cold, hot and alcoholic) of Melissa officinalis (lemon balm) extracts on germination and growth of radish was studied. A field experiment was carried out during October to December 2021 season with Randomized Complete Block Design (RCBD). Alcoholic, hot water and cold water extraction at a concentration of 1 % showed a higher germination percentage compared to other concentrations, while Distilled water showed a major germination percentage 80% compared to other extractions. Regarding the chlorophyll content, alcoholic extraction at a concentration of 0.5 % showed higher Chlorophyll content of 19.5 compared to other treatments and even the control. The root length of radish for alcoholic extraction at a concentration of 1 % showed the highest root length. Alcoholic extraction at a concentration of 0.5 %, hot water extraction at a concentration of 3% and cold-water extraction at a concentration of 5% showed the highest stem diameter. Alcoholic extraction of lemon balm at concentration of 1 presented the biggest leaf diameter. This indicated that the Melissa officinalis extracts as a medicinal plant had different effects on radish germination and growth. Further research should evaluate radish development in different circumstances to see whether this strategy may boost the output of this vital crop.

Scaptomyza flava (Diptera: Drosophilidae) is a serious pest that attacks Brassicaceae, causing significant production losses. The current study was carried out to evaluate the relative efficiency of deltamethrin 2.5 EC, abamectin 1.8 EC, and acetamiprid 20 SP insecticides. In addition to determining the acetamiprid 20 SP and abamectin1.8 EC residues in the leaves and roots of radish. The mortality score revealed that acetamiprid 20 SP, and abamectin 1.8 EC achieved 87.3% and 72.8% respectively after 72 hours compared to deltamethrin 2.5 EC, which scored 54.42%. The chromatogram outcomes of acetamiprid 20 SP and Abamectin1.8EC demonstrated that the acetamiprid 20 SP residues sharply decreased. They reduced from 1856.8 and 25.9 to 0 mg.L-1 in both leaves and roots respectively after ten days of application. While the Abamectin1.8 EC residue was decreasing from 954.12 to 0 mg.L-1 after ten days. Furthermore, abamectin 1.8 EC residues have not been detected in the radish roots. The overall consequence proposed that both acetamiprid 20 SP and abamectin1.8 EC are highly efficient in the controlling of Scaptomyza flava larva and there are no harmful impacts for both on the edible vegetable.

Background.Callus culture in vitro is a convenient model for studying physiological and biochemical manifestations at the cellular level, as well as for cell selection to stress factors. To obtain a callus culture, it is necessary to select the optimal ratio of exogenous hormonal growth regulators (GRs) in the nutrient medium. The purpose of the study is to determine the optimal ratios of GRs at the stage of introducing cucumber and radish into in vitro culture. Materials and methods. Sterile plants of cucumber (Cucumis sativus L., cv. Unity) and radish (Raphanus sativus L., cv. Red Giant) were taken as the object of study. The experiment included obtaining a callus culture of cucumber and radish from various explants (cotyledon leaves, hypocotyl and roots) on a nutrient medium with a mineral base according to Murashige-Skoog supplemented by mesoinositol (100 mg/l), glycine (1 mg/l), nicotinic acid (0.5 mg/l), as well as growth regulators 2.4-dichlorophenoxyacetic acid (2.4- D) and 6-benzylaminopurine (6-BAP) in various ratios. Results. A method for introducing cucumber and radish into in vitro culture is described. The role of the hormonal composition of the nutrient medium in callusogenesis was shown, the influence of different concentrations of GRs, auxin 2.4-D and cytokinin 6-BAP, on callus formation was revealed. For various explants of cucumber and radish, the optimal concentrations of used GRs were determined. Conclusions. The optimal composition of GRs (auxin 2.4-D and cytokinin 6-BAP, mg/l) in Murashige-Skoog medium was selected for callusogenesis on various explants of cucumber (2:2) and radish (4:0.5). A higher intensity of callusogenesis in cucumber on hypocotyl explants and no dependence of callusogenesis on the type of explantin radish were revealed.

Abstract Vegetable crops of the Brassicaceae family have the ability to include the necessary trace element selenium (Se) in the composition of organic compounds such as selenoproteins, in addition, they have important properties for human health based on the content of selenium. In our work, we investigated the effect of non-root processing of vegetating radish plants on the quality of finished products. The research results showed that the selenium content in the product part of plants significantly increased with an increase in the concentration of this element in the working solution. The dry matter content also increased, while its greatest amount was observed at lower concentrations of Se in the working solution. The use of non-root treatment with a selenium-containing solution on vegetative plants led to a significant decrease in the content of ascorbic acid and nitrates, and the decrease in the amount of nitrates in radish root crops was inversely dependent on the concentration of selenium in the working solution.

The present investigation was conducted at the Horticulture Research Farm, [CRC-1] Department of Horticulture, School of Agriculture, ITM University, Gwalior (M.P.), India. Radish (Raphanus sativus L.) is most important root crop is grown widely all over the India, especially near the city markets. It is a popular vegetable in the tropical as well as temperate regions. Radish can cultivated under poly house or glass house for early market but large scale production in field is more common in India. Among various factors responsible for low production of radish, nutrient management is of prime importance for maintaining higher yield and soil fertility. The increasing use of chemical fertilizers to increase vegetable production has been widely recognized but in long run it had detrimental effect on soil health, ecology, natural resources, living organisms including beneficial soil microorganism and human being. Biofertilizers in combination with organic manures found as effective component in organic farming for reliable and cheap supply of nutrients. The experiment was laid out in the Randomized Block Design with three replications. Each replication was comprised of ten treatments consisting phosphate solubilizing bacteria with different RDF doses of fertilizers were applied in radish (Raphanus sativus L.). Result concluded that the different RDF doses of fertilizers with use of phosphate solubilizing bacteria were significantly influence different vegetative and yield parameters of radish. The treatment T7 (30gm/kg P.S.B. + 50:90:50) was found the significantly superior treatment as compared to all other treatments and it also gave the maximum vegetative, yield and economical parameters, whereas the treatment T0 (50:100:50-Control) observed the minimum vegetative, yield and economical parameters rest of all treatments. [ABSTRACT FROM AUTHOR]

During the Advanced Plant Habitat experiment 2, radish plants were grown in two successive grow-outs on the International Space Station (ISS) for 27 days each. On days 10, 18, and 24, leaf punch (LP) samples were collected and frozen. At harvest, bulb tissue was sampled with oligo-dT functionalized Solid Phase Gene Extraction (SPGE) probes. The space samples were compared with samples from ground controls (GC) grown at the Kennedy Space Center (KSC) under the same conditions as on the ISS, with notably elevated CO2 (about 2500 ppm), and from lab plants grown under atmospheric CO2 but with light and temperature conditions similar to the KSC control. Genes corresponding to peroxidase (RPP), glucosinolate biosynthesis (GIS), protein binding (CBP), myrosinase (RMA), napin (RSN), and ubiquitin (UBQ) were measured by qPCR. LP from day 24 and bulb samples collected at harvest were compared with RNA-seq data from material that was harvested, frozen, and analyzed after return to Earth. The results showed stable transcription in LP samples in GC but decreasing values in ISS samples during both grow-outs, possibly indicative of stress. SPGE results were similar between GC and ISS samples. However, the RNA-seq analyses showed different transcription profiles than SPGE or LP results, possibly related to localized sampling. RNA-seq of leaf samples showed greater variety than LP data, possibly because of different sampling times. RSN and RPP showed the lowest transcription regardless of method. Temporal analyses showed relatively small changes during plant development in space and in ground controls. This is the first study that compares developmental changes in space-grown plants with ground controls based on a comparison between RNA-seq and qPCR analyses. [ABSTRACT FROM AUTHOR]

Growing microgreens on trays without substrate in a vertical multilayered growing unit offers several advantages over traditional agriculture methods. This study investigated the yield performance and nutritional quality of five selections of radish microgreens grown in sprouting trays, without a substrate using only water, in an indoor multilayer cultivation system using artificial light. Various parameters were measured, including fresh weight, dry matter, chlorophyll, minerals, amino acids, phenolics, flavonoids, anthocyanins, vitamin C, glucosinolates, and antioxidant activity with four different in vitro assays. After ten days, the biomass had increased by 6-10 times, and the dry matter varied from 4.75-7.65%. The highest yield was obtained from ‘Asia red’, while the lowest was from ‘Koregon red’. However, ‘Koregon red’ and ‘Asia red’ had the highest dry matter. ‘Asia red’ was found to have the highest levels of both Chls and vitamin C compared to the other cultivars, while ‘Koregon red’ exhibited the highest levels of total phenolics and flavonoids. Although variations in the levels of individual glucosinolates were observed, there were no significant differences in the total content of glucosinolates among the five cultivars. ‘Asia purple’ had the highest anthocyanin content, while ‘Asia green 2’ had the lowest. The K, Mg, and Na concentrations were significantly highest in ‘Asia green 2’, and the highest Ca was recorded in ‘Asia purple’. Overall, ‘Asia purple’ and ‘Koregon red’ were the best cultivars in terms of nutritional quality among the tested radish microgreens. These cultivars exhibited high levels of dry weight, total phenolics, flavonoids, anthocyanins, essential and total amino acids, and antioxidant activities. Moreover, the implementation of this vertical cultivation method for microgreens, which relies solely on water and seeds known for their tall shoots during the sprouting could hold promise as a sustainable approach. This method can effectively be utilized for cultivar screening and fulfilling the nutritional and functional needs of the population while minimizing the environmental impacts associated with traditional agriculture practices. [ABSTRACT FROM AUTHOR]

To understand the coloring mechanism in black radish, the integrated metabolome and transcriptome analyses of root skin from a black recombinant inbred line (RIL 1901) and a white RIL (RIL 1911) were carried out. A total of 172 flavonoids were detected, and the analysis results revealed that there were 12 flavonoid metabolites in radish root skin, including flavonols, flavones, and anthocyanins. The relative concentrations of most flavonoids in RIL 1901 were higher than those in RIL 1911. Meanwhile, the radish root skin also contained 16 types of anthocyanins, 12 of which were cyanidin and its derivatives, and the concentration of cyanidin 3-o-glucoside was very high at different development stages of black radish. Therefore, the accumulation of cyanidin and its derivatives resulted in the black root skin of radish. In addition, a module positively related to anthocyanin accumulation and candidate genes that regulate anthocyanin synthesis was identified by the weighted gene co-expression network analysis (WGCNA). Among them, structural genes (RsCHS, RsCHI, RsDFR, and RsUGT75C1) and transcription factors (TFs) (RsTT8, RsWRKY44L, RsMYB114, and RsMYB308L) may be crucial for the anthocyanin synthesis in the root skin of black radish. The anthocyanin biosynthesis pathway in the root skin of black radish was constructed based on the expression of genes related to flavonoid and anthocyanin biosynthesis pathways (Ko00941 and Ko00942) and the relative expressions of metabolites. In conclusion, this study not only casts new light on the synthesis and accumulation of anthocyanins in the root skin of black radish but also provides a molecular basis for accelerating the cultivation of new black radish varieties. [ABSTRACT FROM AUTHOR]

Background: Sugars produced by photosynthesis provide energy for biological activities and the skeletons for macromolecules; they also perform multiple physiological functions in plants. Sugar transport across plasma membranes mediated by the Sugar Will Eventually be Exported Transporter (SWEET) genes substantially affects these processes. However, the evolutionary dynamics and function of the SWEET genes are largely unknown in radish, an important Brassicaceae species. Methods and results: Genome-wide identification and analysis of the RsSWEET genes from the recently updated radish reference genome was conducted using bioinformatics methods. The tissue-specific expression was analyzed using public RNA-seq data, and the expression levels in the bud, stamens, pistils, pericarps and seeds at 15 and 30 days after flowering (DAF) were determined by RT‒qPCR. Thirty-seven RsSWEET genes were identified and named according to their Arabidopsis homologous. They are unevenly distributed across the nine radish chromosomes and were further divided into four clades by phylogenetic analysis. There are 5–7 transmembrane domains and at least one MtN3_slv domain in the RsSWEETs. RNA-seq and RT‒qPCR revealed that the RsSWEETs exhibit higher expression levels in the reproductive organs, indicating that these genes might play vital roles in reproductive organ development. RsSWEET15.1 was found to be especially expressed in siliques according to the RNA-seq data, and the RT‒qPCR results further confirmed that it was most highly expressed levels in the seeds at 30 DAF, followed by the pericarp at 15 DAF, indicating that it is involved in seed growth and development. Conclusions: This study suggests that the RsSWEET genes play vital roles in reproductive organ development and provides a theoretical basis for the future functional analysis of RsSWEETs in radish. [ABSTRACT FROM AUTHOR]

Six sowing dates (September 6th, September 14th, September 22nd, September 30th, October 8th, and October 16th) were set up to study the effects of different sowing dates on the agronomic traits, nutrient accumulation and harvest period of Bairizi' radish. The results showed that with the postponement of the sowing date, the yield of Bairizi radish first increased and then decreased. The 'Bairizi' radish plants sown on September 14-22 grew fully, had high yield, high fruit shape standard, high ascorbic acid and soluble solids content, low lignin content and good quality. Radish fruit phenotype and nutritional quality sown on September 14 had little change, and the harvest period could be as long as 28 days. According to the comprehensive analysis of the agronomic traits, final yield, fruit quality and harvest period of radish under each sowing period, the suitable sowing period of 'Bairizi' radish was September 14-22, and the latest sowing period was September 30. [ABSTRACT FROM AUTHOR]

Corn (Zea mays L.) nitrogen (N) strategies that account for fertilizer timing and source may differentially affect soil bacterial communities and require further validation when preceded by cover crops (CC). Field studies were conducted (2014–2016) evaluating the effects of daikon radish [Raphanus sativus (L.)], forage oat [Avena sativa (L.)], and no CC combined with multiple N management strategies on soil bacterial alpha diversity metrics. Nitrogen strategies consisted of 179 kg N ha−1 applied pre-plant incorporated (PPI), poultry litter (PL) (61 kg N ha−1) applied PPI plus sidedress (SD) N at V11 (11 leaf collars), starter N (45 kg N ha−1) applied 5 cm beside and below the furrow followed by SD at V4 (4 leaf collars), V11, or 50:50 (split) V4 and V11, and a zero N control. In 1 of 2 years, radish reduced diversity 47–53% during active growth compared to remaining treatments. Inverse relationships regarding diversity of in-row (IR) soils at R1 (silking) and grain yield indicated that increased bacterial diversity did not correspond to increased grain production. Relative to PL and the zero-N control, starter N strategies reduced R1 rhizosphere bacterial diversity, evenness, and richness. Combining organic with soluble N sources may stabilize changes to soil alpha diversity metrics at corn silking. [ABSTRACT FROM AUTHOR]

Background : Single nucleotide polymorphisms (SNPs) and insertions/deletions (InDels) are the most abundant genetic variations and widely distribute across the genomes in plant. Development of SNP and InDel markers is a valuable tool for genetics and genomic research in radish (Raphanus sativus L.). Results : In this study, a total of 366,679 single nucleotide polymorphisms (SNPs) and 97,973 insertion-deletion (InDel) markers were identified based on genome resequencing between 'YZH' and 'XHT'. In all, 53,343 SNPs and 4,257 InDels were detected in two cultivars by transcriptome sequencing. Among the InDel variations, 85 genomic and 15 transcriptomic InDels were newly developed and validated PCR. The 100 polymorphic InDels markers generated 207 alleles among 200 Chinese radish germplasm, with an average 2.07 of the number of alleles (Na) and with an average 0.33 of the polymorphism information content (PIC). Population structure and phylogenetic relationship revealed that the radish cultivars from northern China were clustered together and the southwest China cultivars were clustered together. RNA-Seq analysis revealed that 11,003 differentially expressed genes (DEGs) were identified between the two cultivars, of which 5,020 were upregulated and 5,983 were downregulated. In total, 145 flowering time-related DGEs were detected, most of which were involved in flowering time integrator, circadian clock/photoperiod autonomous, and vernalization pathways. In flowering time-related DGEs region, 150 transcriptomic SNPs and 9 InDels were obtained. Conclusions: The large amount of SNPs and InDels identified in this study will provide a valuable marker resource for radish genetic and genomic studies. The SNPs and InDels within flowering time-related DGEs provide fundamental insight into for dissecting molecular mechanism of bolting and flowering in radish. [ABSTRACT FROM AUTHOR]

Selenium is one of the most basic trace elements in the human body. It is necessary to improve the selenium content in agricultural products through agricultural planting technology to ensure that human nutrition and health need selenium. Therefore, our research passed the effect of intercropping on the growth and selenium accumulation of pakchoi, lettuce and radish were studied through pot experiments to determine whether intercropping of the three crop species can improve their selenium accumulation ability. The results showed that intercropping increased the root and shoot biomass of pakchoi and radish compared with the monocultures, while the biomass of roots and shoots decreased in other intercropping combinations. Intercropping also affected the photosynthetic pigment content of the three crop species. Specifically, the photosynthetic pigments increased in pakchoi and decreased in radish after intercropping. Notably, intercropping the three crop species together increased the SOD (superoxide dismutase) activities of the three crops compared with the monocultures. Meanwhile, intercropping radish with lettuce significantly increased the activities of SOD and CAT (catalase) in radish. Intercropping also increased the soluble sugar content in pakchoi and soluble protein content of radish relative to the monocultures. Furthermore, intercropping decreased the selenium content and the bioconcentration factor of the roots of the three vegetable crops, but improved the shoot selenium content, the bioconcentration factor and the transport factor of Se in pakchoi and radish. In conclusion, intercropping combination of pakchoi and radish can improve selenium accumulation in the edible parts of the crops, which is significant for efficient production of selenium-enriched vegetables. This research is significant because this study provides some basis for improving the selenium content of plants and efficient production of pakchoi and radish. Under the condition of selenium application in soil, the intercropping of pakchoi and lettuce can promote the growth of both and improve their selenium enrichment ability. [ABSTRACT FROM AUTHOR]

The adoption of cover crops (CCs) has gained popularity in the continuous corn (Zea mays L.) production system due to their multiple benefits including scavenging or fixing nitrogen (N) in the soil. However, a CC's ability to develop early cover, scavenge N, and provide N to the following cash crop is species‐dependent and affected by environment. A field study was conducted in three diverse environments to determine growth characteristics of nine CC treatments (i.e., monocultures or mixes of grasses, legumes, and brassica), and their effect on the following corn crop was compared to no cover crop treatment (noCC). Cover crops significantly differed for above‐ground biomass, plant tissue carbon (C) and N concentrations, carbon to nitrogen ratio (C/N), and total N uptake (TNU). Among monocultures, grasses had the highest biomass and C/N ratio, and legumes had the highest N concentrations and TNU. Corn grain yield was highest following radish, whereas lowest corn yield was found following cereal rye + crimson clover mix in environment 1. Cover crops varied for C/N ratios in all three environments, but only affected corn plant height (PH) and grain yield in one environment. Cover crops belonging to the same species also exhibit different responses for characteristics measured, depending upon the environment. The expected returns were also variable, especially in CC mixes. The study provides valuable information on the species‐specific functionality of CCs in continuous corn under variable environmental conditions. The information will benefit future studies to explore a high diversity mixture of CCs that may outperform across all three environments. [ABSTRACT FROM AUTHOR]

This study investigated the degradation characteristics and conducted a risk assessment of four pesticides (Diniconazole, Dinotefuran, Metconazole, and Tebuconazole) in the leaves and roots of radish. Radish was cultivated in two greenhouse fields, and samples were collected at 0, 1, 2, 3, 5, 7, and 10 days after pesticide application. Sample analysis was performed using LC-MS/MS, and the recovery rates ranged from 70.1% to 118.6%. The biological half-life of Diniconazole was found to be 6.2 days (leaf and root), Dinotefuran was 5.3 days (leaf) and 4.6 days (root), Metconazole was 9.3 days (leaf) and 3.2 days (root), and Tebuconazole was 8.0 days (leaf) and 5.1 days (root). After comparing the maximum residue limits (MRL) of each pesticide in Korea with the residues during the pre-harvest interval (PHI), Diniconazole showed a Hazard quotient (HQ) exceeding 1, indicating potential risks for true consumers. Furthermore, Tebuconazole showed an HQ of 0.3 or higher, indicating a significant level of risk. [ABSTRACT FROM AUTHOR]

It is becoming increasingly challenging to maintain crop yields and quality as the global climate changes. The aim of this study was to determine whether and how the profile of health-promoting and taste-related compounds of radishes changes within a growing season. A total of 16 radish (Raphanus sativus L.) genotypes that are commercially available on the Czech market were assessed by means of chemical analysis. Radishes were cultivated in three independent growing cycles under controlled conditions, and the effects of the genotype and growing cycle, as well as their interactions, on the chemical traits were evaluated. Most of the variability in chemical composition was associated with the growing cycle, which accounted for 51.53% of total variance, followed by the genotype (26% of total variance). The interaction between the growing cycle and genotype explained 22.47% of total variance. The growing cycle had the strongest effect on amino acid profiles. More specifically, the amino acids that are known to contribute to overall taste (glycine, along with glutamic and aspartic acids) showed the highest degree of variation, while the amino acids related to glucosinolate biosynthesis (methionine, isoleucine, tryptophan, and phenylalanine) showed relatively low variability. On the other hand, indole glucosinolates were found to differ the most between genotypes. [ABSTRACT FROM AUTHOR]

Paucity of knowledge on resistant variety, high yield loss ranging from 32- 57% and dearth of knowledge on management of Alternaria leaf spot has been the major concerns in radish production in the world. Our study will aid in multiplication and standardization of the germane variety of radish resistant to Alternaria leaf spot disease so as to escalate the production, productivity, and the competitiveness in the domestic as well as in the international market. Hence, this experiment was rendered to screen the radish genotypes against Alternaria leaf spot disease in Western Nepal in 2021. Seven genotypes namely Sparkle white F1 hybrid, Korean cross, Palak-patta 35, Pyuthane red, 40 days, Geeta 33, and Mino early were used as the treatment and experiment was laid out in one factorial RCBD design. Each treatment was assigned with 8 plants for observation with 3 replications per treatment. Parameters like days to appearance of disease, incidence of disease on seedling, disease severity (3 scorings), mean score of disease, mean AUDPC and reaction of genotypes were measured during the experiment. Our study unveiled highly significant result for the days to appearance of disease, disease severity, mean score of disease and AUDPC among the genotypes of radish. Alternaria leaf spot first appeared in Pyuthane red (18.33 days) while appeared late in Mino early. Disease severity was found harsh in 40 days at each scoring (57.33, 64.67 and 70.33 respectively); on the contrary severity was minor in Sparkle white at each scoring (26.67, 35 and 44.17 respectively). Similarly, the highest MDS (3.4) and AUDPC (449.8) was found in 40 days, whereas the lowest MDS (1.7) and AUDPC (246.5) was observed in Sparkle white. Ultimately, it is contended that 40 days was found to be moderately susceptible variety while other varieties were found to be moderately resistant.

Radish (Raphanus sativus L.) is a globally significant vegetable and relies on cytoplasmic male sterile (CMS) lines for hybrid seed production. The NWB CMS type is favored over Ogura CMS for its ease in maintainer screening. Despite its varied mitochondrial configurations and unvalidated sterile gene, we re-sequenced the mitochondrial genome of NWB CMS Tibet A and verified the function of the sterility gene via genetic transformation of Arabidopsis thaliana. The mitochondrial genomes of Tibet A could be assembled into circular DNA molecules, with a mitochondrial genome size of 239,184 bp. Our analysis indicated that the specific orf463a was the CMS-associated gene in Tibet A, sharing sequence consistency with the CMS gene in DCGMS and NWB CMS YB-A. Collinearity analysis showed that the mitochondrial genomes of NWB CMS Tibet A, DCGMS, and NWB CMS YB-A share the same mitotype, with structural variations due to recombination via a 9731 bp long repeat sequence and a 508 bp short repeat sequence. Driven by the Ap3 promoter, transgenic Arabidopsis with orf463a exhibited male sterility, confirming the gene’s potential role in CMS. In this study, we assembled a new isomeric form of NWB CMS mitochondrial genome and proved the function of the candidate sterile gene.

This study examined the absorption and concentration of anthracene (AN) in the garden radish. Experiments were conducted to grow radishes from the sowing stage in soil contaminated with AN and to grow radishes in soil contaminated with AN following maturity (27 days after sowing). Regarding growth in the AN-containing soil from sowing onward, the AN concentrations during the growing period in both the soil and radishes decreased exponentially over time, albeit with a time lag. Regarding growth in the AN-containing soil after maturity, the AN concentrations in the roots and leaves had a higher density than those in the stems. In these experiments, positive relationships between the AN levels in the soil and radishes were observed. It was inferred that the greater the degree of soil contamination, the greater the effect was on the amount of AN assimilated by radishes. The concentration ratio (radish-to-soil; Cr/Cs) decreased exponentially with the number of days following AN application. It was interpreted that AN was significantly absorbed in the early stages of radish growth and some of the AN returned to the soil as the growth period progressed. According to the results regarding the change over time in the Cr/Cs content in radish parts, it is suggested that soil AN was well absorbed through the roots to circulate within the organism, and it is preferentially distributed and accumulated in the roots and leaves, which have high organic matter content.

Glucosinolates (GSLs) and their degradation products in radish confer plant defense, promote human health, and generate pungent flavor. However, the intact GSLs in radish have not been investigated comprehensively yet. Here, an accurate qualitative and quantitative analyses of 15 intact GSLs from radish, including four major GSLs of glucoraphasatin (GRH), glucoerucin (GER), glucoraphenin (GRE), and 4-methoxyglucobrassicin (4MGBS), were conducted using UHPLC-HRMS/MS in combination with UHPLC-QqQ-MS/MS. Simultaneously, three isomers of hexyl GSL, 3-methylpentyl GSL, and 4-methylpentyl GSL were identified in radish. The highest content of GSLs was up to 232.46 mmol/g DW at the 42 DAG stage in the 'SQY' taproot, with an approximately 184.49-fold increase compared to the lowest content in another sample. That the GSLs content in the taproots of two radishes fluctuated in a similar pattern throughout the five vegetative growth stages according to the metabolic profiling, whereas the GSLs content in the '55' leaf steadily decreased over the same period. Additionally, the proposed biosynthetic pathways of radish-specific GSLs were elucidated in this study. Our findings will provide an abundance of qualitative and quantitative data on intact GSLs, as well as a method for detecting GSLs, thus providing direction for the scientific progress and practical utilization of GSLs in radish. [ABSTRACT FROM AUTHOR]

Microgreens are emerging specialty crops becoming increasingly popular for their rich nutrient profile and variety of colors, flavors, and textures. The growing medium is a significant key factor in microgreen yield, quality, and sustainability. The widespread use of peat-based media raises questions regarding the environmental sustainability of microgreens production, and new substrates that are more sustainable are required. To this purpose, a study was designed with the objective of comparing eight alternative growing media evaluating their physicochemical properties and effect on yield, mineral profile, and nutritional quality of peas and radish microgreens. Tested substrates included a standard peat and perlite mixture (PP), coconut coir (CC), spent mushroom compost (SMC), organic waste compost (CMP), and 50:50 (v:v) mixes of PP and SMC, PP and CMP, CC and SMC, and CC and CMP. The physicochemical properties widely differed among the alternative substrates tested. SMC had high electrical conductivity and salt concentration, which resulted in poor seed germination. Growing media tested significantly influenced the production and nutritional quality of both microgreen species and variations were modulated by the species. With a 39.8% fresh yield increase or a small yield decrease (-14.9%) in radish and peas, respectively, PP+CMP (50:50, v/v) mix provided microgreens of similar or higher nutritional quality than PP, suggesting the potential of substituting at least in part peat with CMP. Using locally available CMP in mix with PP could reduce the microgreens industry reliance on peat while reducing costs and improving the sustainability of the production of microgreens. Further research is needed to evaluate also the potential economic and environmental benefits of using locally available organic materials like CMP as alternative growing media and peat-substitute to produce microgreens. [ABSTRACT FROM AUTHOR]

Intercropping leguminous plant species with non-legume crops could be an effective strategy to maintain soil fertility. Additionally, the application of nano-Zn and Fe in trace amounts can substantially improve the bioavailable fraction of Zn and Fe. We studied the effect of foliar application of some nanomaterials on the agronomic, physio-biochemical attributes under a radish/pea intercropping system. The radish and pea were sprayed with different nanomaterials (Zn–Fe nanocomposite, nZnO, and nanobiochar) at 0 and 50 mg L-1 concentrations. Results indicated that the growth parameters of radish were higher in intercropping than in monocropping, while pea growth was inhibited in intercropping compared with monocropping. The shoot and root length, fresh weight, and dry matter of radish were increased by 28–50%, 60–70%, and 50–56% by intercropping than monocropping. Foliar spray of nano-materials further increased the growth traits of intercropped radish, such as shoot and root length, fresh weight, and dry matter, by 7–8%, 27–41%, and 50–60%, respectively. Similarly, pigments such as chlorophyll a, b, and carotenoids and the concentration of free amino acids, soluble sugars, flavonoids, and phenolics were differentially affected by intercropping and nanomaterials. The yield of the non-legume crop was increased by intercropping, whereas the legume crop exhibited significant growth inhibition due to competitive interactions. In conclusion, both intercropping and foliar spray of nanomaterials could be used as a combined approach to benefit plant growth and enhance the bioavailable Fe and Zn fractions of both crops. [ABSTRACT FROM AUTHOR]

The research study was executed at Agricultural Research Station Swabi, during winter 2016 on "response of radish (Raphanus sativus L) to various doses of phosphorous and potassium fertilizers". This study was outlined as two factorial, split plot Randomized Complete Block design (RCBD) having three replications. The aim of this study was to observe the effect of macronutrients i.e. P and K on growth and yield of radish crop. Phosphorus at 0 kg ha-1, 25 kg ha-1, 50 kg ha-1, 75 kg ha-1 and potassium at 0 kg ha-1, 30 kg ha-1, 60 kg ha-1, 90 kg ha-1 were applied to radish crop. The effect of different levels on radish crop was evaluated for leaves plant-1, leaf dry matter (%), root dry matter (%), root diameter (mm) and root length (cm). It was observed that there was a significant effect of P and K levels on growth and yield of radish. Results revealed that maximum root diameter, root dry matter, leaf dry matter was recorded in plants fertilized with 25 kg ha-1 phosphorous while highest leaves plant-1 and root length was observed in control plots. Regarding potassium, highest root diameter, leaf dry matter, number of leaves plant-1 was recorded in plots provided with 60 kg ha-1 potassium while maximum root dry matter and root length was observed in 30 kg ha-1. Furthermore, good quality yield of radish can be achieved by providing 50 kg ha-1 phosphorous and 30 kg ha-1. [ABSTRACT FROM AUTHOR]

This study was conducted to evaluate the effect of sodium chloride (NaCl) seed soaking on seed germination and plant growth of cherry radish (Raphanus sativus L.) under saline conditions. Seeds were pretreated by soaking in NaCl solutions at concentrations of 0, 1500, 3000 and 4500 ppm. The pretreated seeds were divided into two groups: the first group was germinated in Petri dishes, and the second group was sown in pots. Both groups were irrigated with different concentrations of salinity (tap water, 1000, 2000 and 3000 ppm NaCl). The results indicated that the salinity of the irrigation water decreased the seed germination percentage and leaf chlorophyll content under a salinity level of 3000 ppm. On the other hand, pretreatment of seeds with NaCl solution led to a gradual increase in germination percentage and total chlorophyll content of leaves. Pretreatment with 4500 ppm NaCl gave comparable values to the effect of control treatment under irrigation conditions with 1000 and 2000 ppm salinity. However, pretreatment of seeds with NaCl led to a gradual decrease in germination percentage, reaching 60% under a salinity level of 3000 ppm, and no obvious effect was seen on the leaf chlorophyll contents. Pretreatment of seeds by NaCl solutions increased the root and shoot fresh and dry weights, while shoot and root weight gradually decreased with the increasing salinity of irrigation water and regardless of the effects attributed to seed pretreatment. The increase in irrigation water salinity also led to a decrease in leaf area, and root length and root diameter, as well as root water contents. In contrast, the salinity led to a gradual increase in leaf specific weight, while the value of leaf number and leaf water contents were not affected. Therefore, the increased specific leaf weight as a result of the salinity of the irrigation water gave an indication that soaking the seeds in saline solutions increased the plant tolerance to salinity that lead to better plant growth. [ABSTRACT FROM AUTHOR]

Glucosinolates (GSLs), precursors of isothiocyanates (ITCs), are present in Brassicaceae plants have been found to have health benefits. Sulforaphane (4-(methylsulfinyl)butyl ITC) is an ITC stored in the formof 4-(methylsulfinyl)butyl GSL (glucoraphanin, 4MSOB) in Brassica vegetables, such as broccoli and kale. Sulforaphane activates Nrf2 expression, a transcription factor responsible for inducing physiological activities such as detoxification in the human body, and it represents a functional component unique to cruciferous vegetables. Raphanobrassica is an inter-generic hybrid between radish and kale, and it contains a high amount of 4MSOB. However, Raphanobrassica contains as much 4-methylsulfinyl-3-butenyl GSL (glucoraphenin, 4MSO3B) as it does 4MSOB. GLUCORAPHASATIN SYNTHASE 1 (GRS1) is an enzyme present in radish that synthesizes 4-methylthio-3-butenyl GSL (glucoraphasatin, 4MT3B), a precursor of 4MSO3B, using 4-(methylthio)butyl GSL (glucoerucin, 4MTB) as a substrate. Since the precursor of 4MSOB is also 4MTB, it was considered that both 4MSOB and 4MSO3B accumulate owing to competition in Raphanobrassica. We hypothesized that owing to the impaired function of GRS1 in Raphanobrassica, it may be possible to breed Raphanobrassica cultivars containing a high 4MSOB content. In this study, we generated Raphanobrassica populations with functional and defective GRS1 and compared the GSL composition in the two populations using high-performance liquid chromatography. The mean 4MSOB content in leaves of the defective-type populations was higher than that in the functionaltype population, and the defective/functional ratio ranged from 2.02 to 2.51-fold, supporting this hypothesis. Furthermore, leaves, flower buds, stems, and roots contained higher amounts of 4MSOB in the defective population than in the functional population. The leaf 4MSOB content of defective Raphanobrassica grown in this study was comparable to that of previously studied vegetables (such as broccoli sprouts) with high 4MSOB content. Raphanobrassica with defective GRS1 represents a new leafy vegetable with high 4MSOB content which exhibits anti-cancerous and anti-inflammatory potentials. [ABSTRACT FROM AUTHOR]

White radish is a nutritious and delectable vegetable that is enjoyed globally. Conventional techniques for monitoring radish growth are arduous and time-consuming, encouraging the development of novel methods for quicker measurements and greater sampling density. This research introduces a mathematical model working on high-resolution images to measure radish's biophysical properties automatically. A color calibration was performed on the dataset using a color checker panel to minimize the impact of varying light conditions on the RGB images. Subsequently, a Mask-RCNN model was trained to effectively segment different components of the radishes. The observations of the segmented results included leaf length, leaf width, root width, root length, leaf length to width, root length to width, root shoulder color, and root peel color. The automated real-life measurements of these observations were then conducted and compared with actual results. The validation results, based on a set of white radish samples, demonstrated the models' effectiveness in utilizing images for quantifying phenotypic traits. The average accuracy of the automated method was confirmed to be 96.2% when compared to the manual method. [ABSTRACT FROM AUTHOR]

Our research group previously found that broccoli sprouts possess neuroprotective effects during pregnancy. The active compound has been identified as sulforaphane (SFA), obtained from glucosinolate and glucoraphanin, which are also present in other crucifers, including kale. Sulforaphene (SFE), obtained from glucoraphenin in radish, also has numerous biological benefits, some of which supersede those of sulforaphane. It is likely that other components, such as phenolics, contribute to the biological activity of cruciferous vegetables. Notwithstanding their beneficial phytochemicals, crucifers are known to contain erucic acid, an antinutritional fatty acid. The aim of this research was to phytochemically examine broccoli, kale, and radish sprouts to determine good sources of SFA and SFE to inform future studies of the neuroprotective activity of cruciferous sprouts on the fetal brain, as well as product development. Three broccoli: Johnny's Sprouting Broccoli (JSB), Gypsy F1 (GYP), and Mumm's Sprouting Broccoli (MUM), one kale: Johnny's Toscano Kale (JTK), and three radish cultivars: Black Spanish Round (BSR), Miyashige (MIY), and Nero Tunda (NT), were analyzed. We first quantified the glucosinolate, isothiocyanate, phenolics, and DPPH free radical scavenging activity (AOC) of one-day-old dark- and light-grown sprouts by HPLC. Radish cultivars generally had the highest glucosinolate and isothiocyanate contents, and kale had higher glucoraphanin and significantly higher sulforaphane content than the broccoli cultivars. Lighting conditions did not significantly affect the phytochemistry of the one-day-old sprouts. Based on phytochemistry and economic factors, JSB, JTK, and BSR were chosen for further sprouting for three, five, and seven days and subsequently analyzed. The three-day-old JTK and radish cultivars were identified to be the best sources of SFA and SFE, respectively, both yielding the highest levels of the respective compound while retaining high levels of phenolics and AOC and markedly lower erucic acid levels compared to one-day-old sprouts. [ABSTRACT FROM AUTHOR]

The presented study, held at the Research cum Instructional Farm of the College of Horticulture and Research Station, Jagdalpur, Chhattisgarh, India, ran during the Rabi season of 2021, to investigate superior radish cultivars for selection having high yield potential and better quality root for Bastar plateau. The experiment, laid out in randomized block design (RBD), had 15 treatments and three replications. Observing high genotypic and phenotypic coefficient of variation was for the following traits: dry and fresh weights of roots, plants, and leaves; root and leaf yields; days to 50% germination; the diameter and length of the root, North-South and East-West spread of the plant; plant height, and the number of leaf plant-1, having recorded significant heritability and high genetic advance. Similarly, high heritability showed for root yield and fresh and dry weights of roots. Moderate heritability levels appeared for the traits, viz., the plant's dry weight and the root diameter. The genetic gain resulted high for characters, viz., root yield and fresh and dry weights of roots. High heritability followed by high genetic advance indicates that selection may be effective for improving such characteristics. Root yield (t ha-1) exhibited a highly significant positive correlation with the fresh weight of the plant at the phenotypic and genotypic levels, followed by dry weight of roots, dry weight of the plant, East-West spread of the plant, the diameter of the root, and leaf yield plot-1. The fresh weight of roots had the maximum positive direct effect on root yield in the genotypic path, followed by the dry weight of roots and the plant, the East-West spread of the plant, the diameter of the root, and leaf yield in radish. The selection of these characters can benefit in improving the yield of radishes. [ABSTRACT FROM AUTHOR]

SWEET (Sugars Will Eventually be Exported Transporter) genes play essential roles in various biological processes, including phloem loading, sugar efflux, plant development and stress response. In this study, a total of 33 RsSWEET gene members were identified in the radish genome. They could be divided into four subfamilies and are distributed on eight radish chromosomes. Cis-acting regulatory element analysis indicated that these RsSWEET genes were potentially involved in the radish growth and development and stress response process, including circadian control and light response and responses to numerous stresses, including low-temperature and drought stress. Transcriptome data analysis revealed that a number of RsSWEET genes exhibited specific expression patterns in different tissues and developmental stages of radish. Moreover, several RsSWEET genes (e.g., RsSWEET2a, RsSWEET3a, RsSWEET16b and RsSWEET17) showed differential expression profiles under various abiotic stresses, including cold, heat, salt, Cd and Pb stress. Remarkably, the RsSWEET17 was specifically expressed in the cambium of radish. RsSWEET17 was heterologously expressed in yeast strain EBY.VW4000, which suggested that it has the ability to transport sugar. Notably, RsSWEET17-overexpressing Arabidopsis plants exhibited excessive root length, greater fresh weight and higher soluble sugar content (SSC) accumulation compared with wild-type (WT) plants, indicating that RsSWEET17 might positively regulate radish taproot development by strategically manipulating sugar accumulation. Collectively, these results clarify the molecular mechanisms underlying RsSWEET-mediated sugar accumulation and root growth and development in radish. [ABSTRACT FROM AUTHOR]

Vegetables, which are an important part of human nutrition, are very rich in minerals necessary for human health, and heavy metals can be found in vegetables at high rates because they can be easily taken by plant roots and leaves. In this study, the macro, micro element and heavy metal element concentrations accumulated in different parts of some carrot and radish types were investigated. The element concentrations in the samples were analyzed by Inductively coupled plasma optical emission spectrometry (ICP-OES; Varian-Vista Model) equipment. P, K, Ca, Mg and S contents of the head of orange and black carrot samples were determined as 602.30 and 727.23 mg/kg, 19,790.91 and 22,230.21 mg/kg, 1765.66 and 1609.41 mg/kg, 580.34 and 660.79 mg/kg and 376.21 and 4444.46 mg /kg respectively. Also, exterior parts of orange and black carrots contained 281.65 and 336.43 mg/kg P, 7768.37 and 10,109.44 mg/kg K, 169.88 and 272.18 mg/kg Ca, 112.08 and 189.28 mg/kg Mg and 135.43 and 217.60 mg/kg S, respectively. P and K contents of the head parts of radish samples (white, red and black radish) were determined between 302.14 (red radish) and 1111.53 mg/kg (black radish) to 13,717.2 (red radish) and 22,202.4 mg/kg (white radish), respectively. Fe amounts of the roots of radish samples changed between20.47 (red radish) and 45.93 mg/kg (white radish). As and Ba were the most abundant heavy metals in both carrot and radish parts. The Ni contents of the parts of the carrots contain more than 50% lower than the head part. Also, while Pb contents of the parts of orange carrot change between 0.189 µg/g (interior of body) and 0.976 µg/g (shell), Pb amounts of the black carrot parts were recorded between 0.136 (head) and 0.536 µg/g (interior of body). The results obtained differed according to the vegetable type and parts. The head part of the radishes was the richest in zinc, followed by root, shell, exterior of body and interior of body in descending order. In general, the parts where heavy metals were most localized were the head and shell parts. The most localized parts of heavy metals in radishes were the head, shell and root parts. As a result, the most of the edible inner parts of carrots and radishes are thought to have a positive effect on human health, since their heavy metal content is low. [ABSTRACT FROM AUTHOR]

Radish is a vegetable high in nutritional and phytochemical contents that are beneficial to the human body, especially for managing kidney stone disease. However, it is less consumed as compared to other vegetables due to its pungent flavour and odour. Therefore, the objective of the present work was to formulate a radish effervescent tablet to enhance the palatability of the vegetable. The radish effervescent tablet was formulated by using Simplex Lattice Mixture Design where the percentages of sucralose and strawberry flavour were chosen as the factors. Five formulations were generated, and sensory acceptance test was conducted on them. Next, the halalan toyyiban principle compliance was evaluated based on the Halal Control Points (HCPs), toxicity assay (brine shrimp lethality assay), anti-urolithiatic properties (turbidimetric assay), and nutritional composition (energy, total protein, total fat, total carbohydrate, and total sugar). Formulation 5 (F5) with 20% citric acid, 12% sodium bicarbonate, 58% radish powder, 2.5% sucralose, and 7.5% strawberry flavour was selected as the most acceptable formulation (p < 0.05). For the halalan toyyiban principle compliance, F5 was evaluated, and it complied with the halalan toyyiban principles. It is halal, non-toxic, and safe for consumption as the LC50 was greater than 1,000 µg/mL (2,223.31 µg/mL) for toxicity test, and exhibited significant potential as an anti-urolithiatic agent (88.13% inhibition). In the future, prototype development of radish effervescent tablet with potential anti-urolithiatic and fast-dissolving properties can be carried out. [ABSTRACT FROM AUTHOR]

Radish is a popular root vegetable grown worldwide and young fresh tender tuberous roots can be cooked or eaten raw in salads. The haphazard use of inorganic fertilizers for long period deteriorates fertility and productivity of soil. Therefore, this study was proposed for an extensive evaluation of different combinations of organic manure, bio fertilizer with inorganic fertilizers and influence on growth, yield, quality, and soil health. This study was carried out on radish cv. Japanese white with 13 treatment combinations in randomized block design with three replications. Soil quality was increased in terms of availability of N(Nitrogen), P (Phosphorus) and K (Potassium) to the tune of 15.17, 18.66 and 13.0%, respectively by integrated module 90% RDF (Recommended dose of fertilizers) + 10% SMC (Spent Mushroom Compost) + FYM (Farm Yard Manure) + AZB (Azotobacter) + PSB (Phosphate Solubilising Bacteria). Application of 90% Recommended dose of Fertilizers +10% Spent Mushroom Compost + Farm Yard Manure + Azotobacter + Phosphate Solubilising Bacteriarecorded maximum number of leaves (15.68), leaf size (21.01 x 9.88 cm), root size (18.80 x 2.86 cm), root weight (130.42 g), root yield (341.68q/ha) while, 60% Recommended dose of fertilizers +40% Spent Mushroom Compost+ Farm Yard Manure + Azotobacter + Phosphate Solubilising Bacteria proved best for quality attributes. Increase in yield under T6 was to the tune 11.44% over standard practices. Our findings of integrated nutrient management approaches may effectively address the present notion of "farming for health" to maintain soil health for sustainable crop production. [ABSTRACT FROM AUTHOR]

Interspecific hybridization and accompanying backcross between crops and relatives have been recognized as a powerful method to broaden genetic diversity and transfer desirable adaptive traits. Crosses between radish (Raphanus sativus, RR, 2n = 18) and Brassica oleracea (CC, 2n = 18), which formed allotetraploid Raphanobrassica (RRCC, 2n = 36), initiated the construction of resynthetic allopolyploids. However, these progenies from the backcrosses between Raphanobrassica and the two diploid parents have not been well deciphered. Herein, thousands of backcrosses using both Raphanobrassica and the two diploid parents as pollen donors were employed. Several hybrids with expected (2n = 27) and unexpected chromosome numbers (2n = 26 and 2n = 36) were obtained. Fluorescence in situ hybridization (FISH) analysis with R-genome-specific sequences as probes demonstrated that the genome structures of the two expected hybrids were RRC and CCR, and the genome structures of the three unexpected hybrids were RRRC, CCCR, and RRC' (harbouring an incomplete C genome). The unexpected hybrids with extra R or C genomes showed similar phenotypic characteristics to their expected hybrids. FISH analysis with C-genome-specific sequences as probes demonstrated that the unexpected allotetraploid hybrids exhibited significantly more intergenomic chromosome pairings than the expected hybrids. The expected and unexpected hybrids provide not only novel germplasm resources for the breeding of radish and B. oleracea but also very important genetic material for genome dosage analysis. [ABSTRACT FROM AUTHOR]

Background and Purpose: Fungal infection by species of pathogenic Candida withantifungal resistance is currently a serious problem. Treatment with new medications isbecoming more challenging to manage this type of infection. The present study aimed to investigate the antifungal effect of essential oils (EOs) against itraconazole-resistantspecies of pathogenic Candida.Materials and Methods: Seven essential oils were tested on 15 clinical isolates ofitraconazole-resistant Candida from patients with vulvovaginal candidiasis. Theantifungal action of selected EOs was evaluated using the disc diffusion method with the determination of the minimum inhibitory concentration (MIC) of effective EOs.Results: Radish EO was the most effective type against all Candida isolates with MICsbetween 3.125% and 6.25% (v/v) .It also had a stronger effect than itraconazole. Sixother EOs showed antifungal effects at varying concentrations and were dependent upon the type of isolate. Low concentrations of these six EOs were more effective against many isolates than their high concentrations. Moreover, camphor and linseed EOs were less effective on isolates.Conclusion: Radish EO has a strong antifungal activity against itraconazole-resistancespecies of Candida, even more than itraconazole. The antifungal action of some EOs can be increased through the use of low concentrations.

Research on combined phytotoxicity of perfluorooctanoic acid (PFOA) and nanoparticles is very important for the remediation of PFOA contaminated soil and further assessment for the potential of nano-enhanced phytoremediation. Here, joint effects of PFOA and CuO nanoparticles (nCuO) in plants were studied by exposing radish (Raphanus sativus L.) to PFOA (4 mg/kg) and nCuO (200 and 400 mg/kg) for 30 days, and measuring for contaminant accumulation, radish biomass, photosynthesis profiles and nutrient contents. Results showed that PFOA accumulated highly in radish organs but showed limited effects on radish biomass. nCuO could increase the transfer rate of PFOA from root to shoot and reduce PFOA accumulation in edible root part, but higher nCuO lead to decreased radish biomass. Reversely, PFOA alleviated the adverse effects of nCuO on leaf photosynthesis and root metabolism of vitamins and amino acids. These results provided basics for exploring possibility of nano-enhanced phytoremediation for PFOA soil pollution.