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The aim of this study was to evaluate, ex vivo, the histological effects of 445-nm diode laser (Eltech K-Laser srl, Treviso, Italy), during an oral soft tissue biopsy. Thirty samples from pig cadaver tongues were obtained, through five laser settings, in continuous and pulsed wave (CW and PW). Samples were divided into six groups of five pieces each. A control specimen was taken by a scalpel. All samples were examined with an optical microscope by a blinded pathologist. Thermal effects on epithelium and connective tissues were measured with LAS 4.8 software. Finally, a statistical evaluation was made using GraphPadPrism 7.0 software. All specimens, except one, showed a damage lower than 1 mm. Readability was always optimal; there was a different thermal effect between epithelial and connective tissue and in CW and PW samples, confirmed by statistical analysis too. A 445-nm diode laser creates a minimum thermal effect, that has no implications in the histological evaluation of benign lesions. In suspicious lesions, a safety margin of 1 mm, compared with a scalpel, is preferable. A 445-nm diode laser has excellent surgical properties and can manage many arduous clinical cases, such as vascularized lesions. In the excision of suspected lesions, it is necessary to compare, case by case, clinical advantages to possible histological implications.

Amaç: Bu retrospektif çalışmanın amacı, belirli bir zaman aralığında yapılan oral mukoza lezyonlarının biyopsi sonuçlarını değerlendirmektir. Gereç ve Yöntemler: 2016-2018 yılları arasında Altınbaş Üniversitesi ve Kocaeli Üniversitesi Diş Hekimliği Fakülteleri Oral Diagnoz Kliniklerinde yapılan oral mukoza lezyonlarının biyopsi sonuçları retrospektif olarak incelenmiştir. Bu çalışmada, hastaların demografik bilgileri, kullanılan biyopsi tekniği, lezyonların lokalizasyonu ve histopatolojik tanıları değerlendirilmiştir. Bulgular: Çalışmamıza katılan yaşları 16 ile 73 arasında değişen 36 hastanın 21'i kadın, 15'i erkektir. En fazla biyopsisi yapılmış oral mukoza lezyonları 5 olguda irritasyon fibromu ve 5 olguda papillomdur. Yapılan biyopsi yöntemine göre sınıflama yaptığımızda 25 olguda eksizyonel, 6 olguda insizyonel, 4 olguda punch, 1 olguda smear biyopsisi yapılmıştır. Biyopsilerin alındığı bölge olarak biyopsilerin en sık 13 olguda yanak mukozasından, 6 olguda alt dudaktan, 5 olguda dilden, 4 olguda sert damaktan alındığı görülmektedir. Sonuç: Oral Diagnoz uzmanlarının ağız içinde görülen selim, premalign ve malign lezyonların erken teşhisinde önemli sorumlulukları mevcuttur. Bu lezyonların doğru tanısında histopatolojik ve sitolojik muayene gibi tanıya yardımcı metotlar gerekli olabilmektedir., Background: The aim of this retrospective study was to analyze the results of the oral mucosa biopsies performed at a certain time interval. Methods: A retrospective study was carried out investigating the results of oral mucosa biopsies performed in Oral Diagnosis Clinics of Altınbaş and Kocaeli Universities, Dentistry Faculties, between 2016-2018 years. In this study, demographic data, the method of biopsy, location and the histopathological diagnosis of the lesions were evaluated. Results: Of the 36 patients aged between 16 to 73 included in our study, 21 of the patients were female, and 15 were male. The most commonly biopsied oral mucosa lesions were irritation fibroma in 5 cases and papilloma in 5 cases. According to the biopsy method, excisional biopsies in 25 cases, incisional biopsies in 6 cases, punch biopsies in 4 cases and smear biopsy in 1 case were performed. Localizations of biopsies were buccal mucosa in 13 cases, lower lip in 6 cases, tongue in 5 cases and hard palate in 4 cases. Conclusion: Oral diagnosis specialists have important duties in the early detection of benign, premalignant and malignant lesions in the mouth. Accurate diagnosis of these lesions can be performed by additional diagnostic methods such as histopathological and cytological examination.

Aim: The aim of this study is to describe an unusual case of an Epidermoid Cyst (EC) of the tongue treated by a Potassium Titanium Phosphate (KTP) laser. Presentation of Case: A 38 young male patient came to our attention since a little painless swelling, yellowish, on the lateral left side of the tongue. After an accurate clinical examination, a surgical excision with a KTP laser was performed. Control at 7 and 21 days showed a complete healing of lesion and the histological diagnosis was EC. Discussion: EC generally appear like a firm, usually painless swelling that sometimes raises the tongue. In the differential diagnosis of the EC the clinician has to consider a wide range of pathologic conditions that could be classified as development, neoplastic and infective pathologies. Surgical enucleation is the only effective treatment for these kinds of lesions and prognosis is very good, with a very low incidence of relapse. Conclusion: Several techniques are reported in the literature but the use of KTP laser for the excisional biopsy of ORAL EC is very effective, since the relative ease and speed of execution, the compliance of patience, the absence of bleeding and suture.

Many oral lesions may need to be diagnosed by removing a sample of tissue from the oral cavity. Biopsy is widely used in the medical field, but the practice is not quite widespread in dental practice. As oral pathologists, we have found many artifacts in the tissue specimen because of poor biopsy technique or handling, which has led to diagnostic pitfalls and misery to both the patient and the clinician. This article aims at alerting the clinicians about the clinical faults arising preoperatively, intraoperatively and postoperatively while dealing with oral biopsy that may affect the histological assessment of the tissue and, therefore, the diagnosis. It also reviews the different techniques, precautions and special considerations necessary for specific lesions.

Made available in DSpace on 2019-09-11T20:51:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2011 The aim of this paper is to describe a case of a relatively common oral pathological lesion which diagnosis became "complex" because of previous biopsies performed without observing all technical requirements. This is a female patient, 49 years old, showing sessile submucosal tumor in the right lower lip with a history of 10 years of evolution. On clinical examination there was a lesion about 3. cm in diameter, painless, with the overlying mucosa erythematous and with decreased tactile sensitivity. Featured wine-stains on his right elbow and back. Patient underwent two biopsies and used several drugs without clinical improvement or definite diagnosis because the pathologic reports, because of lack of representativeness of the biopsy material, showed only chronic inflammatory process. After differential diagnosis made by a dentist and a dermatologist it was decided to perform a third biopsy, excisional this time, which allowed a definitive diagnosis of the lesion. © 2011 Sociedade Portuguesa de Estomatologia e Medicina Dentária. Carvalho De Melo, A.U., Doutor Titular da Disciplina de Estomatologia da Universidade Tiradentes (UNIT/SE), Brazil Ferreira Ribeiro, C., Programa de Programa de Pós-Graduação em Odontologia (Doutorado), Universidade de Taubaté (UNITAU), Brazil De Santana Santos, T., Programa de Pós-Graduação em Odontologia (Doutorado), Universidade de São Paulo (FORP/USP), Brazil Oliveira Neto, A., Aluno de Graduação do curso de Odontologia da Universidade Tiradentes (UNIT/SE), Brazil Nascimento Araújo, F.E., Aluno de Graduação do curso de Odontologia da Universidade Tiradentes (UNIT/SE), Brazil Cavalcanti Albuquerque Júnior, R.L., Doutor Titular da Disciplina de Patologia Oral e Maxilofacial da Universidade Tiradentes (UNIT/SE), Brazil

Celiac disease (CD) is a lifelong, T cell—mediated enteropathy, triggered by the ingestion of gluten and related prolamins in genetically susceptible subjects, resulting in minor intestinal mucosal injury, including villous atrophy with crypt hyperplasia and intraepithelial lymphocytosis, and subsequent nutrient malabsorption. Although serological tests for antiendomysial (EMA) and anti—tissue transglutaminase (anti-tTG) autoantibodies are used to screen and follow up on patients with CD, diagnostic confirmation is still based on the histological examination of the small intestinal mucosa. Although the small intestinal mucosa is the main site of the gut involved in CD, other mucosal surfaces (such as gastric, rectal, ileal, and esophageal) belonging to the gastrointestinal tract and the gut-associated lymphoid tissue (GALT) can also be involved. A site that could be studied less invasively is the mouth, as it is the first part of the gastrointestinal system and a part of the GALT. Indeed, not only have various oral ailments been reported as possible atypical aspects of CD, but it has been also demonstrated that inflammatory changes occur after oral supramucosal application and a submucosal injection of gliadin into the oral mucosa of CD patients. However, to date, only two studies have assessed the capacity of the oral mucosa of untreated CD patients to EMA and anti-tTG antibodies. In this paper, we will review studies that evaluate the capacity of the oral mucosa to produce specific CD autoantibodies. Discrepancies in sensitivity from the two studies have revealed that biopsy is still not an adequate procedure for the routine diagnostic purposes of CD patients, and a more in-depth evaluation on a larger sample size with standardized collection and analysis methods is merited. However, the demonstration of immunological reactivity to the gluten ingestion of the oral mucosa of CD, in terms of IgA EMA and anti-tTG production, needs to be further evaluated in order to verify whether the oral mucosa is colonized by lymphocytes activated in the intestine or if gluten could stimulate naïve lymphocytes directly in the oral mucosa. This would have important implications for the pathogenesis, diagnosis, and treatment of CD.

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:24:43Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:30:46Z : No. of bitstreams: 1 2-s2.0-77958123471.pdf: 451667 bytes, checksum: 4e6dfbedf99b5ca0bd0b6dd5d3566db6 (MD5) Made available in DSpace on 2014-05-27T11:24:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-07-01 Mucocele forms because of salivary gland mucous extravasation or retention and is usually related to trauma in the area of the lower lips. Ruptured ducts release the mucous that accumulates into adjacent tissues, leading to swelling. This report describes a large mucocele involving the lower lip, which was produced in a child by incorrect use of a pacifier. A few important concepts are discussed to help clinicians in the diagnosis and treatment of this pathology. © 2010 Dermatology Online Journal. Department of Orthodontics and Pediatric Dentistry Clinic State University of São Paulo São José dos Campos Dental School - UNESP Department of Orthodontics and Pediatric Dentistry Clinic State University of São Paulo São José dos Campos Dental School - UNESP

Es este artículo se revisa el papel de la biopsia oral en el diagnóstico de lesiones precancerosas y en el diagnóstico precoz del cáncer oral. Se discuten diferentes técnicas, procedimientos, materiales, indicaciones y aspectos quirúrgicos. Se propone efectuar biopsias incisionales en lesiones malignas y sospechosas de malignidad, en tanto se preconizan biopsias escisionales, cuando el tamaño lo permita, en lesiones precancerosas. This article reviews the paper of oral biopsy on the precancerous lesions diagnosis and on the oral cancer early diagnosis. Different techniques, procedures, materials, indications and other surgical aspects are debated. It proposes to do incisional biopsies on malignant lesions and on malignant suspicious lesions, while doing excisional biopsies on precancerous lesions when the size allows it.

The conclusions drawn from the study of an oral biopsy are considered essential for the definitive diagnosis of diseases of the oral mucosa, and for the subsequent planning of appropriate treatment. Although the obtainment of biopsies is widely used in all medical fields, the practice is not so widespread in dental practice - fundamentally because of a lack of awareness of the procedure among dental professionals. In this context, it must be taken into account that the early diagnosis of invasive oral malignancy may be critical for improving the patient prognosis. However, in some cases the results are adversely affected by incorrect manipulation of the biopsy material. The present study provides an update on the different biopsy sampling techniques and their application. Such familiarization in turn will contribute to knowledge of the material and instruments required for correct biopsy performance in dentistry, as well as of the material required for correct sample storage and transport. La utilización de la biopsia oral se considera una prueba irrefutable para el diagnóstico definitivo de enfermedades de la mucosa bucal y la subsiguiente planificación de un enfoque terapéutico adecuado. Aunque esta técnica está ampliamente extendida en todas las especialidades médicas, en el ámbito de la odontología no está muy divulgada, fundamentalmente por el desconocimiento de la misma por parte del odontólogo general; y cabe tener en consideración que un diagnóstico temprano de un cáncer oral invasivo pueda mejorar de forma radical el pronóstico del paciente. Sin embargo, en ocasiones el resultado se ve malogrado por un inadecuado manejo del material a analizar. El objetivo de este trabajo es actualizar las diversas técnicas para obtener la muestra y su aplicación. Nos facilitará, en gran medida, conocer el material e instrumental que precisemos para su toma en odontología y el material más apropiado para la conservación y el transporte de la muestra.

The aim of the KOALA Birth Cohort Study in the Netherlands is to identify factors that influence the clinical expression of atopic disease with a main focus on lifestyle (e.g., anthroposophy, vaccinations, antibiotics, dietary habits, breastfeeding and breast milk composition, intestinal microflora composition, infections during the first year of life, and gene-environment interaction). The recruitment of pregnant women started in October 2000. First, participants with 'conventional lifestyles' (n = 2343) were retrieved from an ongoing prospective cohort study (n = 7020) on pregnancy-related pelvic girdle pain. In addition, pregnant women (n = 491) with 'alternative lifestyles' with regard to child rearing practices, dietary habits (organic, vegetarian), vaccination schemes and/or use of antibiotics, were recruited through organic food shops, anthroposophic doctors and midwives, Steiner schools, and dedicated magazines. All participants were enrolled between 14 and 18 wk of gestation and completed an intake questionnaire on family history of atopy and infant care intentions. Documentation of other relevant variables started in the pregnant mother and covered the first and third trimester as well as early childhood by repeated questionnaires at 14-18, 30, and 34 wk of gestation and 3, 7, 12, and 24 months post-partum. A subgroup of participants, including both conventional and alternative lifestyles, was asked to consent to maternal blood sampling, breast milk and a faecal sample of the infant at 1 month post-partum, capillary blood at age 1 yr, venous blood and observation of manifestation of atopic dermatitis during home visits at the age of 2 yr (using the UK working party criteria and the severity scoring of atopic dermatitis index), and buccal swabs for DNA isolation from child-parent trios. From the start, ethical approval and informed consent procedures included gene-environment interaction studies. Follow-up at 3 and 7 months post-partum was completed with high response rates (respectively 90% and 88% in the conventional group, and 97% and 97% in the alternative group). The home visits at 2 yr of age will be completed in 2005. Preliminary results show that we have succeeded in recruiting a large population with various lifestyle choices with a fairly large contrast with regard to dietary habits (including organic foods, vegetarian diet), vaccination schemes and/or use of antibiotics. We have also been able to collect a large number of faecal samples (n = 1176) and capillary blood samples at age 1 yr (n = 956). Furthermore, a large proportion of the participants have consented with genetic studies. Mid 2006 we expect to report our first results on the relationship between the various exposures in early life and childhood atopy. An outline of the focus and design of the KOALA Birth Cohort Study is presented. © 2005 Blackwell Munksgaard.

PG - 679-84 AB - The aim of the KOALA Birth Cohort Study in the Netherlands is to identify factors that influence the clinical expression of atopic disease with a main focus on lifestyle (e.g., anthroposophy, vaccinations, antibiotics, dietary habits, breastfeeding and breast milk composition, intestinal microflora composition, infections during the first year of life, and gene-environment interaction). The recruitment of pregnant women started in October 2000. First, participants with 'conventional lifestyles' (n = 2343) were retrieved from an ongoing prospective cohort study (n = 7020) on pregnancy-related pelvic girdle pain. In addition, pregnant women (n = 491) with 'alternative lifestyles' with regard to child rearing practices, dietary habits (organic, vegetarian), vaccination schemes and/or use of antibiotics, were recruited through organic food shops, anthroposophic doctors and midwives, Steiner schools, and dedicated magazines. All participants were enrolled between 14 and 18 wk of gestation and completed an intake questionnaire on family history of atopy and infant care intentions. Documentation of other relevant variables started in the pregnant mother and covered the first and third trimester as well as early childhood by repeated questionnaires at 14-18, 30, and 34 wk of gestation and 3, 7, 12, and 24 months post-partum. A subgroup of participants, including both conventional and alternative lifestyles, was asked to consent to maternal blood sampling, breast milk and a faecal sample of the infant at 1 month post-partum, capillary blood at age 1 yr, venous blood and observation of manifestation of atopic dermatitis during home visits at the age of 2 yr (using the UK working party criteria and the severity scoring of atopic dermatitis index), and buccal swabs for DNA isolation from child-parent trios. From the start, ethical approval and informed consent procedures included gene-environment interaction studies. Follow-up at 3 and 7 months post-partum was completed with high response rates (respectively 90% and 88% in the conventional group, and 97% and 97% in the alternative group). The home visits at 2 yr of age will be completed in 2005. Preliminary results show that we have succeeded in recruiting a large population with various lifestyle choices with a fairly large contrast with regard to dietary habits (including organic foods, vegetarian diet), vaccination schemes and/or use of antibiotics. We have also been able to collect a large number of faecal samples (n = 1176) and capillary blood samples at age 1 yr (n = 956). Furthermore, a large proportion of the participants have consented with genetic studies. Mid 2006 we expect to report our first results on the relationship between the various exposures in early life and childhood atopy. An outline of the focus and design of the KOALA Birth Cohort Study is presented. AD - Department of Epidemiology, Care and Public Health Research Institute (Caphri), Maastricht University, Maastricht, The Netherlands. ischa.kummeling@epid.unimaas.nl

Oral soft tissue lesions are commonly seen in the daily dental practice. The quick diagnosis of oral potentially malignant disorders and oral precancer disease is of highest clinical importance given the mortality rate of late stage disease. Since the oral cavity is more accessible to complete examination, it could be used in early detection of precancerous and cancerous lesions. But either due to ignorance or inaccessibility of medical care, the disease gets detected in the later stages. Thus, there is a need for improvement in early detection of oral disease, because in the initial stages, treatment is more effective and the morbidity is minimal. Aim of this report is to highlight how collagen membrane graft application seems to offer a perfect healing of the soft tissue after the lesion removal. Some reports and the microstructure of the collagen used have been recorded. The healing of the soft tissue, the bleeding control and the management of postoperative discomfort seem to be more favorable by avoiding a intraoral soft tissue graft and applying a collagen membrane. Oral soft tissue lesions are commonly seen in the daily dental practice. The quick diagnosis of oral potentially malignant disorders and oral precancer disease is of highest clinical importance given the mortality rate of late stage disease. Since the oral cavity is more accessible to complete examination, it could be used in early detection of precancerous and cancerous lesions. But either due to ignorance or inaccessibility of medical care, the disease gets detected in the later stages. Thus, there is a need for improvement in early detection of oral disease, because in the initial stages, treatment is more effective and the morbidity is minimal. Aim of this report is to highlight how collagen membrane graft application seems to offer a perfect healing of the soft tissue after the lesion removal. Some reports and the microstructure of the collagen used have been recorded. The healing of the soft tissue, the bleeding control and the management of postoperative discomfort seem to be more favorable by avoiding a intraoral soft tissue graft and applying a collagen membrane.

Introduction The frequency of pediatric and adolescent oral and maxillofacial lesions is various in different societies. The present study aimed at investigating the frequency of oral and maxillofacial pediatric and adolescent biopsies in Zahedan (southeast Iran), and compare the results with other epidemiologic studies.Methods and Materials This retrospective study reviewed oral and maxillofacial lesions in patients with 0-18 years old referring to the treatment centers of Zahedan University of Medical Sciences during 12-years period. Patients’ demographic information including age, gender and location of the lesion were collected and statistically analyzed.ResultsIn general, among 1112 oral and maxillofacial lesions, 154 (13.9%) cases were related to children and adolescents younger than 18 years old. The average age of patients was 11.4 ± 4.9, 53.2% and 46.8% of them were boys and girls, respectively. The most frequent sites of lesions were the gingiva and lip. The most prevalent lesions included inflammatory/reactive, cystic and neoplastic lesions, respectively. Benign and malignant tumors comprised 12.3% and 4.5% of cases. Moreover, pyogenic granuloma and peripheral giant cell granuloma (PGCG) were the most frequent lesions.ConclusionThe most prevalent oral and maxillofacial lesions in patients under 18 years old were inflammatory/reactive lesions in gingiva and in the 13-18 age range. Determining the characteristics of these lesions in the children and adolescents population provides a firm groundwork for proper diagnosis and treatment.