51. 不同剂量天麻素对甲基苯丙胺依赖 CPP 大鼠海马中 TLR4/MyD88/NF-κB 炎症信号通路的影响.
- Author
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朱婷娜, 曹媛媛, 张 园, 刘鹏亮, 王一航, 吴亚梅, 李利华, 赵永娜, and 洪仕君
- Abstract
Objective To investigate the effects of different dosages of gastrodin onTLR4/ MyD88 / NFκB signal pathway in the hippocampus of methamphetamine (meth)dependence rats. Methods The conditioned place preference (CPP) model of meth was established, meth (10mg/kg) was administered (i.p, 14 d), and then, different dosages (10, 30, 100 mg/kg) of gastrodin were used to intervene in 14 days. Western blotting and RTqPCR were used to explore the expression level of protein and mRNA of key factors in TLR4/ MyD88 / NF-κB signal pathway in hippocampus. Results Gastrodin decreased the protein and mRNA expression of TLR4, MyD88, TRAF6, NF-κBp65 in a dose-dependent manner (P < 0.001, P < 0.01 or P < 0.05), and the protein expression of p-NF-κB p65 was reduced with showing a significant dose-response relationship ( P < 0.01 or P < 0.05) . Gastrodin increased the protein and mRNA expression of IκB-α with showing a significant dose-response relationship ( P < 0.01 or P < 0.05) . Gastrodin reduced the protein expression of p-IκB-α ( P < 0.01) . Conclusion Gastrodin intervention has a protective effect against hippocampal neuroinflammation in methamphetamine-dependent CPP rats, and is closely related to the function of TLR4/MyD88/NF-κB signal pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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