51. Maternal supplementation with edible birds' nest during gestation and lactation enhances intestinal barrier function by upregulating Claudin-1 in rat offspring.
- Author
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Zhang, Wen, Zhao, Guoping, Lin, Xiaoxian, Li, Chuangang, Zhu, Huiqing, Bi, Ran, Fang, Bing, Xiong, Wei, Yuan, Man, Wang, Dongliang, and Li, Yixuan
- Abstract
[Display omitted] • Maternal edible bird's nest (EBN) supplementation promoted intestinal barrier integrity in offspring. • Maternal EBN supplementation increased the number of goblet cells in offspring. • Maternal EBN supplementation improved the expression of tight junction proteins in offspring including ZO-1, Occludin and Claudin-1. • Maternal EBN supplementation upregulated the expression of RUNX3 and TGF-β, the Claudin-1 upstreaming signaling pathway proteins. • EBN might upregulate the expression of TGF-β by modulating intestinal microbiota, leading to elevated expression of the transcription factor RUNX3, ultimately resulting in the upregulation of Claudin-1. Maternal diet is indispensable for the proper development of offspring, and the establishment of a well-functioning intestinal barrier is crucial for normal growth and development. Edible bird's nest (EBN) has positive impacts on intestinal health, but the underlying mechanisms are unknown. Our study showed that maternal EBN supplementation downregulated the serum levels of diamine oxidase and D-lactate, elevated expression of tight junction proteins ZO-1, Occludin, and Claudin-1, and regulated the intestinal microbiota in rat offspring. Spearman's correlation analysis revealed that Claudin-1, transforming growth factor-β (TGF-β), and transcription factor RUNX3 were positively correlated with changes in intestinal bacteria. EBN may upregulate the expression of TGF-β by modulating the intestinal microbiota composition, enhancing RUNX3 binding to the Claudin-1 promoter region to upregulate its expression. In conclusion, we demonstrated that maternal EBN supplementation during gestation and lactation enhanced intestinal barrier function by upregulating Claudin-1 in rat offspring. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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