108 results on '"de Bem AF"'
Search Results
52. Succinobucol, a Lipid-Lowering Drug, Protects Against 3-Nitropropionic Acid-Induced Mitochondrial Dysfunction and Oxidative Stress in SH-SY5Y Cells via Upregulation of Glutathione Levels and Glutamate Cysteine Ligase Activity.
- Author
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Colle D, Santos DB, Hartwig JM, Godoi M, Engel DF, de Bem AF, Braga AL, and Farina M
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- Buthionine Sulfoximine pharmacology, Cell Death drug effects, Cell Line, Tumor, Cell Survival drug effects, Glutamate-Cysteine Ligase genetics, Glutathione Peroxidase metabolism, Humans, Hydroquinones pharmacology, Mitochondria drug effects, Nitro Compounds, Probucol pharmacology, Propionates, Protective Agents pharmacology, RNA, Messenger genetics, RNA, Messenger metabolism, Time Factors, Glutamate-Cysteine Ligase metabolism, Glutathione metabolism, Hypolipidemic Agents pharmacology, Mitochondria metabolism, Oxidative Stress drug effects, Probucol analogs & derivatives, Up-Regulation drug effects
- Abstract
Succinobucol (succinyl ester of probucol) is a lipid-lowering compound with anti-inflammatory and antioxidant properties. Recent experimental evidence has highlighted the potential neuroprotective effects of succinobucol. In the present study, cultured neuroblastoma (SH-SY5Y) cells were used to investigate mechanisms mediating the potential protective effect of succinobucol against mitochondrial metabolic impairment and oxidative stress induced by 3-nitropropionic acid (3-NP), a succinate dehydrogenase inhibitor that has been used in experimental models of the Huntington disease (HD). 3-NP decreased cellular viability after 24 h of incubation. This decline in cellular viability was preceded by (i) reduced mitochondrial complex II activity, (ii) increased reactive species generation, (iii) decreased mitochondrial membrane potential (ΔΨm), and (iv) diminished glutathione (GSH) levels. Succinobucol pretreatment (6 days) significantly prevented 3-NP-induced loss of cellular viability, generation of reactive oxygen species, and decrease of ΔΨm. However, succinobucol pretreatment did not protect against 3-NP-induced inhibition of mitochondrial complex II activity, pointing to the mitigation of secondary events resultant from mitochondrial complex II inhibition. Succinobucol pretreatment (6 days) significantly increased (50 %) the levels of GSH in SH-SY5Y cells, and this event was paralleled by significant increases in glutamate cysteine ligase messenger RNA (mRNA) expression and activity (GCL; the first enzyme in the GSH biosynthesis). The present findings are the first to show that succinobucol increases GSH levels via upregulation of GCL activity (possibly through the activation of the nuclear (erythroid-derived 2)-related factor (Nrf2)/antioxidant response element (ARE) pathway), displaying protective effects against mitochondrial dysfunction-derived oxidative stress.
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- 2016
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53. Long-term and low-dose malathion exposure causes cognitive impairment in adult mice: evidence of hippocampal mitochondrial dysfunction, astrogliosis and apoptotic events.
- Author
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dos Santos AA, Naime AA, de Oliveira J, Colle D, dos Santos DB, Hort MA, Moreira EL, Suñol C, de Bem AF, and Farina M
- Subjects
- Animals, Apoptosis drug effects, Astrocytes pathology, Cholinesterase Inhibitors toxicity, Dose-Response Relationship, Drug, Hippocampus pathology, Male, Mice, Mitochondria drug effects, Mitochondria pathology, Spatial Memory drug effects, Toxicity Tests, Chronic methods, Astrocytes drug effects, Cognition Disorders chemically induced, Hippocampus drug effects, Insecticides toxicity, Malathion toxicity
- Abstract
The organophosphorus (OP) pesticide malathion is a neurotoxic compound whose acute toxicity is primarily caused by the inhibition of acetylcholinesterase (AChE), leading to cholinergic syndrome-related symptoms. Some lines of evidence indicate that long-term exposure to low levels of OP may produce neuropsychiatric and/or neurobehavioral signs that do not necessarily involve the AChE inhibition. This study evaluated the effects of a repeated (15-day period) and low-dose malathion exposure on spatial memory and discrimination (object location task), as well as on biochemical parameters in the hippocampus of mice [AChE and mitochondrial chain complexes activities; levels of proapoptotic proteins (Bax and Bak) and cholinergic neuronal and astroglial markers (ChAT and GFAP, respectively)]. Malathion treatments (30 and 100 mg/kg, s.c.) did not affect the body weight of animals and caused no evident signs of cholinergic toxicity throughout the treatment, although the highest dose (100 mg/kg) was associated with inhibition of AChE activity. Malathion-exposed animals showed a significant impairment on spatial memory and discrimination, which was correlated with a decrease in the mitochondrial complex I activity in the hippocampus. Moreover, malathion increased the levels of proapoptotic proteins and induced astroglial activation. The results show that long-term malathion exposure, at a dose that does not affect hippocampal AChE activity (30 mg/kg), caused impaired spatial memory and discrimination in mice that was related to hippocampal mitochondrial dysfunctional, astrogliosis and apoptosis. When extrapolated to humans, such results shed light on noncholinergic mechanisms likely related to the neurobehavioral and cognitive deficits observed in individuals chronically exposed to this pesticide.
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- 2016
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54. Efficacy of Donepezil for Cognitive Impairments in Familial Hypercholesterolemia: Preclinical Proof of Concept.
- Author
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Lopes JB, de Oliveira J, Engel DF, de Paula GC, Moreira EL, and de Bem AF
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- Animals, Cholinesterase Inhibitors pharmacology, Cognition Disorders etiology, Discrimination, Psychological drug effects, Disease Models, Animal, Donepezil, Drug Evaluation, Preclinical, Exploratory Behavior drug effects, Hyperlipoproteinemia Type II complications, Hyperlipoproteinemia Type II psychology, Learning drug effects, Memory drug effects, Mice, Inbred C57BL, Mice, Knockout, Psychological Tests, Receptors, LDL genetics, Receptors, LDL metabolism, Space Perception drug effects, Cognition Disorders drug therapy, Hyperlipoproteinemia Type II drug therapy, Indans pharmacology, Nootropic Agents pharmacology, Piperidines pharmacology
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- 2015
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55. Cerebral cortex, hippocampus, striatum and cerebellum show differential susceptibility to quinolinic acid-induced oxidative stress.
- Author
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Vandresen-Filho S, Martins WC, Bertoldo DB, Mancini G, De Bem AF, and Tasca CI
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- Analysis of Variance, Animals, Brain anatomy & histology, Cerebellum drug effects, Cerebellum physiology, Cerebral Cortex drug effects, Cerebral Cortex physiology, Corpus Striatum drug effects, Corpus Striatum physiology, Glutathione Peroxidase metabolism, Glutathione Reductase metabolism, Hippocampus drug effects, Hippocampus physiology, Lipid Peroxidation drug effects, Male, Mice, Reactive Oxygen Species metabolism, Thiobarbituric Acid Reactive Substances metabolism, Time Factors, Brain drug effects, Brain physiology, Oxidative Stress drug effects, Quinolinic Acid toxicity, Seizures chemically induced
- Abstract
Quinolinic acid (QA) is a NMDA receptor agonist implicated in pathological conditions, such as neurodegenerative diseases and epilepsy. Time-course responses of different brain regions after QA i.c.v. infusion are not known. We aimed to investigate the time-course effects of QA infusion on oxidative stress-related parameters on different brain regions. In cerebral cortex, QA infusion promoted an early (1 h) decrease of NPSH levels and GR activity followed by a later increase in ROS production (8 h) and TBARS detection (24-72 h). In the hippocampus, QA promoted an increase in ROS production that lasted 8 h. Striatal tissue presented a later increase in ROS generation (8-72 h) after QA infusion. In the cerebellum, an increase in the GPx activity after 8 h was the only effect observed. These results show that oxidative stress induced by QA i.c.v. infusion is region and time dependent.
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- 2015
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56. Impact of SIN-1-derived peroxynitrite flux on endothelial cell redox homeostasis and bioenergetics: protective role of diphenyl diselenide via induction of peroxiredoxins.
- Author
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Fiuza B, Subelzú N, Calcerrada P, Straliotto MR, Piacenza L, Cassina A, Rocha JB, Radi R, de Bem AF, and Peluffo G
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- Animals, Aorta cytology, Cattle, Endothelial Cells enzymology, Homeostasis drug effects, Membrane Potential, Mitochondrial drug effects, Mitochondria enzymology, Molsidomine chemistry, Oxidation-Reduction, Peroxynitrous Acid chemistry, Peroxynitrous Acid toxicity, Benzene Derivatives pharmacology, Endothelial Cells drug effects, Energy Metabolism drug effects, Mitochondria drug effects, Molsidomine analogs & derivatives, Organoselenium Compounds pharmacology, Peroxiredoxins metabolism, Peroxynitrous Acid metabolism
- Abstract
Increased production of reactive nitrogen (RNS) and oxygen (ROS) species and its detrimental effect to mitochondria are associated with endothelial dysfunction. This study was designed to determine the effect of a peroxynitrite flux, promoted by 1,3-morpholinosydnonimine (SIN-1), in mitochondrial function and some redox homeostasis parameters in bovine aortic endothelial cells (BAEC). Moreover, the effect of diphenyl diselenide (PhSe)2, a simple organic selenium compound, in preventing peroxynitrite-mediated cytotoxicity was also investigated. Our results showed that overnight exposure to SIN-1 (250 μM) caused a profound impairment of oxygen consumption, energy generation and reserve capacity in mitochondria of BAEC. Mitochondrial dysfunction resulted in an additional intracellular production of peroxynitrite, amplifying the phenomenon and leading to changes in redox homeostasis. Moreover, we observed an extensive decline in mitochondrial membrane potential (ΔΨm) induced by peroxynitrite and this event was associated with apoptotic-type cell death. Alternatively, the pretreatment of BAEC with (PhSe)2, hindered peroxynitrite-mediated cell damage by preserving mitochondrial and endothelial function and consequently preventing apoptosis. The protective effect of (PhSe)2 was related to its ability to improve the intracellular redox state by increasing the expression of different isoforms of peroxiredoxins (Prx-1-3), efficient enzymes in peroxynitrite detoxification.
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- 2015
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57. Probucol mitigates streptozotocin-induced cognitive and biochemical changes in mice.
- Author
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Santos DB, Colle D, Moreira ELG, Peres KC, Ribeiro RP, Dos Santos AA, de Oliveira J, Hort MA, de Bem AF, and Farina M
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- Acetylcholinesterase metabolism, Administration, Oral, Alzheimer Disease, Amyloid Precursor Protein Secretases metabolism, Animals, Antioxidants pharmacology, Aspartic Acid Endopeptidases metabolism, Cholesterol blood, Disease Models, Animal, Glutathione Peroxidase metabolism, Glutathione Reductase metabolism, Male, Mice, Oxidative Stress drug effects, Oxidative Stress physiology, Recognition, Psychology drug effects, Recognition, Psychology physiology, Streptozocin, Cognition Disorders drug therapy, Cognition Disorders metabolism, Hippocampus drug effects, Hippocampus metabolism, Nootropic Agents pharmacology, Probucol pharmacology
- Abstract
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by synaptic loss and cognitive impairments. Although AD is the most prevalent aging-related neurodegenerative disease, therapeutic strategies remain palliative. Recent studies have shown that probucol presents neuroprotective effects in experimental models of neurodegenerative disease. The present study aimed to investigate the potential protective effects of probucol against streptozotocin (STZ)-induced cognitive impairment and hippocampal biochemical changes (oxidative stress-related parameters, acetylcholinesterase (AChE) activity, cholesterol levels and β-secretase (BACE) protein levels) in mice. Adult Swiss mice received STZ [150 μg/bilateral, i.c.v.], and treated daily with probucol (≅10 mg/kg/day, in drinking water, for 5 weeks,). Twenty-one days after i.c.v. administrations, STZ-infused animals displayed significant deficits in cognition (evaluated in the displaced and new object recognition tasks), which were paralleled by a significant increase in hippocampal AChE activity. Moreover, STZ-infused mice showed increased levels of BACE and decreased glutathione reductase (GR) activity in the hippocampus compared with the control group. Probucol treatment significantly protected against the behavioral and hippocampal biochemical changes induced by STZ. However, it was unable to prevent STZ-induced increase of hippocampal BACE levels and did not change hippocampal cholesterol levels. It is noteworthy that probucol treatment increased the glutathione peroxidase (GPx) activity per se independent of STZ injection. The present findings are the first to show that i.c.v. STZ infusions are able to increase hippocampal BACE expression. Moreover, the results also show that probucol can counteract STZ-induced cognitive impairments and biochemical parameters independently of potential modulator effects toward BACE levels. The study is the first to report the protective effects of probucol against STZ-induced biochemical hippocampal changes and behavioral impairments, rendering this compound a promising molecule for further pharmacological studies on the search for therapeutic strategies to treat or prevent AD., (Copyright © 2014 IBRO. Published by Elsevier Ltd. All rights reserved.)
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- 2015
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58. β-Caryophyllene protects the C6 glioma cells against glutamate-induced excitotoxicity through the Nrf2 pathway.
- Author
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Assis LC, Straliotto MR, Engel D, Hort MA, Dutra RC, and de Bem AF
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- Animals, Astrocytes physiology, Cell Line, Tumor, Cell Survival drug effects, Cell Survival physiology, Dose-Response Relationship, Drug, Glutathione metabolism, Glutathione Peroxidase metabolism, Membrane Potential, Mitochondrial drug effects, Membrane Potential, Mitochondrial physiology, Mitochondria drug effects, Mitochondria physiology, Polycyclic Sesquiterpenes, Rats, Reactive Oxygen Species metabolism, Astrocytes drug effects, Glutamic Acid toxicity, NF-E2-Related Factor 2 metabolism, Neuroprotective Agents pharmacology, Sesquiterpenes pharmacology
- Abstract
β-Caryophyllene (BCP), a natural bicyclic sesquiterpene present in several essential oils, displays analgesic and anti-inflammatory properties in vitro and in vivo. Astrocytes are a major class of glial cells that regulate extracellular ion balance, repair and scarring processes in the CNS following neuroinflammatory conditions and traumatic injuries. This study sought to determine the protective effect of BCP against glutamate (Glu)-induced cytotoxicity in the C6 glioma cell line on neurochemical parameters as well as their biochemical mechanism. Glu increases intracellular reactive oxygen species (ROS) production and induces mitochondrial dysfunction as well as decreasing antioxidant defenses such as glutathione (GSH) and glutathione peroxidase activity. BCP prevented C6 cells from Glu-induced cytotoxicity by modulating the cellular antioxidant response, mainly by inhibiting ROS production and reestablishing the mitochondrial membrane potential (Δψm). Moreover, BCP per se induced the nuclear translocation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) which was reflected by improvement in the cellular GSH antioxidant system. Taken together, our results suggest that cytoprotective effects of BCP were mediated by the amelioration of cellular antioxidant responses via Nrf2 activation, which is, in part, dependent on cannabinoid receptor type 2 (CB2R) activation. This functional nonpsychoactive CB2R ligand, could represent an important molecule for protection of glial cells against oxidative stress induced by glutamate., (Copyright © 2014 IBRO. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2014
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59. Diphenyl diselenide protects endothelial cells against oxidized low density lipoprotein-induced injury: Involvement of mitochondrial function.
- Author
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Hort MA, Straliotto MR, de Oliveira J, Amoêdo ND, da Rocha JB, Galina A, Ribeiro-do-Valle RM, and de Bem AF
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- Animals, Apoptosis drug effects, Atherosclerosis etiology, Atherosclerosis metabolism, Cattle, Cell Survival drug effects, Endothelial Cells pathology, Glutathione metabolism, Humans, Lipoproteins, LDL metabolism, Lipoproteins, LDL toxicity, Mitochondria drug effects, Oxidative Stress drug effects, Protective Agents metabolism, Reactive Oxygen Species metabolism, Atherosclerosis drug therapy, Benzene Derivatives administration & dosage, Endothelial Cells drug effects, Organoselenium Compounds administration & dosage, Protective Agents administration & dosage
- Abstract
Elevated levels of oxidized low density lipoprotein (oxLDL) are considered to be one of the major risk factors for atherosclerosis and cardiovascular morbidity. The early stages of atherosclerosis are initiated by the accumulation of oxLDL and the induction of toxic effects on endothelial cells, resulting in endothelial dysfunction. The aim of this study was to investigate how diphenyl diselenide (DD), an organoselenium compound, protect vascular endothelial cells against the toxic effects of oxLDL in vitro. Our data showed that the treatment of bovine endothelial aortic cells (BAEC) with DD (0.1-1 μM) for 24 h protected from oxLDL-induced reactive species (RS) production and reduced glutathione (GSH) depletion. Moreover, DD (1 μM) per se improved the maximal mitochondrial respiratory capacity and prevented oxLDL-induced mitochondrial damage. In addition, DD could prevent apoptosis induced by oxLDL in BAEC. Results from this study may provide insight into a possible molecular mechanism underlying DD suppression of oxLDL-mediated vascular endothelial dysfunction., (Copyright © 2014 Elsevier Masson SAS. All rights reserved.)
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- 2014
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60. Diphenyl diselenide improves the antioxidant response via activation of the Nrf-2 pathway in macrophage cells.
- Author
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Mancini G, Raniel Straliotto M, da Rocha JB, and de Bem AF
- Abstract
Diphenyl diselenide [(PhSe)2] is an organoselenium compound that can mimic endogenous antioxidant enzymes, such as glutathione peroxidase (GPx), or be metabolized by thioredoxin reductase to form selenol intermediate, which can copy the function of the antioxidant selenoenzymes. This compound has shown potential role in preventing atherosclerosis and other oxidative stress-related diseases. The understanding of the underlying mechanism by which (PhSe)2 modulates the glutathione-related antioxidant defenses is a relevant question. Therefore, we tested its ability to promote the nuclear translocation of the nuclear factor (erythroid 2-like)-related factor 2 (Nrf-2), increasing the expression of enzymes related to the antioxidant system, such as heme oxygenase 1 (HO-1) and peroxiredoxin 1 (Prx-1), in addition to the main enzyme in the glutathione synthesis - gamma glutamylcysteine synthetase (?-GCS) - in murine J774 macrophage cells. (PhSe)2 (1µM) was able to promote nuclear translocation and increased the expression of the Nrf-2 factor in the nucleus in a time-dependent manner (1-24hours). In addition, this compound significantly increased the expression of HO-1 and Prx-1 at 24hours and GPx-1 after the first hour. Furthermore, (PhSe)2 was able to enhance GSH levels in a time-dependent manner, as well as GPx and GGCS activities. The increase in GPx and GGCS activities was dependent on the activation of PI3K, JNK, and p38MAPKs signaling pathways that may activate the Nrf2 factor. Altogether, these results show that (PhSe)2 improved the antioxidant defense by increasing the expression of HO-1 and Prx-1 and the synthesis of GSH as a consequence of the activation and nuclear translocation of Nrf-2 factor., (Copyright © 2014. Published by Elsevier Inc.)
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- 2014
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61. Low-density lipoprotein receptor: a promising therapeutic target to promote cerebral beta-amyloid clearance?
- Author
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de Oliveira J, de Bem AF, and Moreira EL
- Subjects
- Amyloid beta-Peptides pharmacology, Animals, Humans, Mice, Alzheimer Disease drug therapy, Alzheimer Disease metabolism, Alzheimer Disease pathology, Cerebral Cortex metabolism, Receptors, LDL metabolism
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- 2014
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62. Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model.
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Glaser V, Martins Rde P, Vieira AJ, Oliveira Ede M, Straliotto MR, Mukdsi JH, Torres AI, de Bem AF, Farina M, da Rocha JB, De Paul AL, and Latini A
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- Animals, Brain pathology, Cerebral Cortex drug effects, Cerebral Cortex metabolism, Disease Models, Animal, Male, Mercury Poisoning, Nervous System metabolism, Mercury Poisoning, Nervous System pathology, Methylmercury Compounds toxicity, Mice, Mitochondria drug effects, Oxidative Stress drug effects, Benzene Derivatives administration & dosage, Brain metabolism, Heme Oxygenase-1 biosynthesis, Mitochondria metabolism, Organoselenium Compounds administration & dosage
- Abstract
Interest in biochemistry of organoselenium compound has increased in the last decades, mainly due to their chemical and biological activities. Here, we investigated the protective effect of diphenyl diselenide (PhSe)2 (5 μmol/kg), in a mouse model of methylmercury (MeHg)-induced brain toxicity. Swiss male mice were divided into four experimental groups: control, (PhSe)2 (5 μmol/kg, subcutaneous administration), MeHg (40 mg/L, in tap water), and MeHg + (PhSe)2. After the treatment (21 days), the animals were killed and the cerebral cortex was analyzed. Electron microscopy indicated an enlarged and fused mitochondria leading to a reduced number of organelles, in the MeHg-exposed mice. Furthermore, cortical creatine kinase activity, a sensitive mitochondrial oxidative stress sensor, was almost abolished by MeHg. Subcutaneous (PhSe)2 co-treatment rescued from MeHg-induced mitochondrial alterations. (PhSe)2 also behaved as an enhancer of mitochondrial biogenesis, by increasing cortical mitochondria content in mouse-receiving (PhSe)2 alone. Mechanistically, (PhSe)2 (1 μM; 24 h) would trigger the cytoprotective Nrf-2 pathway for activating target genes, since astroglial cells exposed to the chalcogen showed increased content of hemeoxygenase type 1, a sensitive marker of the activation of this via. Thus, it is proposed that the (PhSe)2-neuroprotective effect might be linked to its mitoprotective activity.
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- 2014
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63. Neuroprotective effect of the proanthocyanidin-rich fraction in experimental model of spinal cord injury.
- Author
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Assis LC, Hort MA, de Souza GV, Martini AC, Forner S, Martins DF, Silva JC, Horst H, dos Santos AR, Pizzolatti MG, Rae GA, Koepp J, de Bem AF, and do Valle RM
- Subjects
- Animals, Antioxidants pharmacology, Antioxidants therapeutic use, Cell Death drug effects, Disease Models, Animal, Ganglia, Spinal drug effects, Ganglia, Spinal metabolism, Male, Movement drug effects, Muscle Strength drug effects, Neuroprotective Agents pharmacology, Plant Bark, Plant Extracts pharmacology, Plant Extracts therapeutic use, Proanthocyanidins pharmacology, Rats, Wistar, Reactive Oxygen Species metabolism, Spinal Cord Injuries physiopathology, Croton chemistry, Glutamic Acid adverse effects, Neuroprotective Agents therapeutic use, Phytotherapy, Proanthocyanidins therapeutic use, Receptors, Glutamate metabolism, Spinal Cord Injuries drug therapy
- Abstract
Objectives: In this study, we evaluated the effect of the proanthocyanidins-rich fraction (PRF) obtained from Croton celtidifolius bark in an experimental animal model of spinal cord injury and cell death induced by glutamate., Methods: Experiments were conducted using adult male Wistar rats (10 weeks old and weighing 270-300g). Experimental groups were randomly allocated into the following groups: spinal cord injury (SCI) + vehicle group: rats were subjected to SCI plus intraperitoneal administration of vehicle (saline 10 ml/kg); SCI + PRF: rats were subjected to SCI plus intraperitoneal administration of PRF (10 mg/kg) at 1 and 6 h after injury and sham operated., Key Findings: The treatment with the proanthocyanidin-rich fraction significantly improved not only motor recovery and grip force but also H2 O2 or glutamate-induced cell death and reactive oxygen species generation induced by glutamate in dorsal root ganglion cells. In this study we demonstrate that the neuroprotective effect triggered by the proanthocyanidins-rich fraction appears to be mediated in part by the inhibition of N-methyl-D-aspartate-type glutamate receptors., Conclusions: Taken together, our results demonstrate that PRF treatment ameliorates spinal cord injury and glutamatergic excitotoxicity and could have a potential therapeutic use., (© 2014 Royal Pharmaceutical Society.)
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- 2014
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64. Hypercholesterolemia induces short-term spatial memory impairments in mice: up-regulation of acetylcholinesterase activity as an early and causal event?
- Author
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Moreira EL, de Oliveira J, Engel DF, Walz R, de Bem AF, Farina M, and Prediger RD
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- Amyloid Precursor Protein Secretases metabolism, Analysis of Variance, Animals, Aquaporin 4 metabolism, Aspartic Acid Endopeptidases metabolism, Blood Glucose metabolism, Cell Line, Tumor, Disease Models, Animal, Glutathione metabolism, Glutathione Reductase metabolism, Hippocampus enzymology, Lipids blood, Male, Maze Learning physiology, Mice, Neuroblastoma pathology, Prefrontal Cortex enzymology, Swimming psychology, Thiobarbituric Acid Reactive Substances metabolism, Acetylcholinesterase metabolism, Hypercholesterolemia complications, Memory Disorders etiology, Memory Disorders metabolism, Up-Regulation physiology
- Abstract
Epidemiological studies have indicated hypercholesterolemia in midlife as a risk factor for dementia in later life, bringing cholesterol to the forefront of Alzheimer's disease research. Herein, we modeled mild hypercholesterolemia in mice to evaluate biochemical and behavioral alterations linked to hypercholesterolemia. Swiss mice were fed a high fat/cholesterol diet (20 % fat and 1.25 % cholesterol) for an 8-week period (from 12 to 18 weeks old) and were tested on the object location, forced swimming and elevated plus-maze tasks. We also investigated hypercholesterolemia-induced changes on acetylcholinesterase (AChE) activity, oxidative damage, amyloid precursor protein (APP) processing and blood brain barrier (BBB) integrity within the prefrontal cortex and hippocampus. It was found that increased AChE activity within the prefrontal cortex and hippocampus is an early event associated with hypercholesterolemia-induced short-term memory impairments. We observed no signs of antioxidant imbalance and/or oxidative damage or changes in cortical and hippocampal densities of beta-site amyloid precursor protein-cleaving enzyme 1 and aquaporin-4, biomarkers of APP processing and BBB integrity, respectively. In addition, we treated SH-SY5Y human neuroblastoma cells with low-density lipoprotein (LDL) cholesterol in an attempt to manipulate cell cholesterol content. Notably, LDL cholesterol increased in a dose-dependent manner the activity of AChE in SH-SY5Y cells. The present findings provide new evidence that increased AChE activity within the prefrontal cortex and hippocampus is an early event associated with hypercholesterolemia-induced cognitive impairments.
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- 2014
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65. Diphenyl diselenide differently modulates cardiovascular redox responses in young adult and middle-aged low-density lipoprotein receptor knockout hypercholesterolemic mice.
- Author
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Mancini G, de Oliveira J, Hort MA, Moreira EL, Ribeiro-do-Valle RM, Rocha JB, and de Bem AF
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- Age Factors, Animals, Antioxidants metabolism, Aorta, Atherosclerosis prevention & control, Glutathione metabolism, Glutathione Reductase metabolism, Malondialdehyde metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, Mitochondria drug effects, Mitochondria metabolism, Oxidation-Reduction, Reactive Oxygen Species metabolism, Thiobarbituric Acid Reactive Substances, Antioxidants pharmacology, Benzene Derivatives pharmacology, Heart drug effects, Hypercholesterolemia metabolism, Myocardium metabolism, Organoselenium Compounds pharmacology, Oxidative Stress drug effects, Receptors, LDL metabolism
- Abstract
Objectives: The present work aimed to investigate the effect of (PhSe)2 on cardiovascular age-related oxidative stress in hypercholesterolemic mice., Methods: To this end, LDL receptor knockout (LDLr(-/-) ) mice, 3 months (young adult) and 12 months (middle-aged) old, were orally treated with (PhSe)2 ., Key Findings: Hypercholesterolemia, regardless of age, impaired the mitochondrial antioxidant defence in the cardiac tissue, which was characterized by a decline in mitochondrial aortic glutathione (GSH) levels and increased reactive oxygen species production in the heart. (PhSe)2 treatment improved GSH levels, thioredoxin reductase (TRxR) and GSH reductase (GR) activity, and decreased malondialdehyde levels in the heart of young adult LDLr(-/-) mice. Moreover, (PhSe)2 increased GPx activity in both age groups, and GR activity in the aorta of middle-aged LDLr(-/-) mice., Conclusions: Therefore, (PhSe)2 enhances the antioxidant defences in the cardiovascular system of LDLr(-/-) mice, which could explain its success as an anti-atherogenic compound., (© 2013 Royal Pharmaceutical Society.)
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- 2014
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66. Increased susceptibility to amyloid-β-induced neurotoxicity in mice lacking the low-density lipoprotein receptor.
- Author
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de Oliveira J, Moreira EL, dos Santos DB, Piermartiri TC, Dutra RC, Pinton S, Tasca CI, Farina M, Prediger RD, and de Bem AF
- Subjects
- Acetylcholinesterase metabolism, Amyloid beta-Peptides, Amyloidosis complications, Amyloidosis pathology, Animals, Antioxidants metabolism, Astrocytes pathology, Astrocytes physiology, Blood-Brain Barrier physiopathology, Capillary Permeability physiology, Cell Membrane Permeability physiology, Disease Models, Animal, Gliosis pathology, Gliosis physiopathology, Hippocampus pathology, Memory Disorders etiology, Memory Disorders pathology, Memory, Short-Term physiology, Mice, Inbred C57BL, Mice, Knockout, Neuroimmunomodulation physiology, Oxidative Stress physiology, Peptide Fragments, Prefrontal Cortex pathology, Receptors, LDL genetics, Spatial Memory physiology, Superoxide Dismutase metabolism, Amyloidosis physiopathology, Hippocampus physiopathology, Memory Disorders physiopathology, Prefrontal Cortex physiopathology, Receptors, LDL metabolism
- Abstract
Familial hypercholesterolemia is caused by inherited genetic abnormalities that directly or indirectly affect the function of the low-density lipoprotein (LDL) receptor. This condition is characterized by defective catabolism of LDL which results in increased plasma cholesterol concentrations and premature coronary artery disease. Nevertheless, there is increasing preclinical and clinical evidence indicating that familial hypercholesterolemia subjects show a particularly high incidence of mild cognitive impairment. Moreover, the LDL receptor (LDLr) has been implicated as the main central nervous system apolipoprotein E receptor that regulates amyloid deposition in distinct mouse models of β-amyloidosis. In this regard, herein we hypothesized that the lack of LDLr would enhance the susceptibility to amyloid-β-(Aβ)-induced neurotoxicity in mice. Using the acute intracerebroventricular injection of aggregated Aβ(1-40) peptide (400 pmol/mouse), a useful approach for the investigation of molecular mechanisms involved in Aβ toxicity, we observed oxidative stress, neuroinflammation, and neuronal membrane damage within the hippocampus of C57BL/6 wild-type mice, which were associated with spatial reference memory and working memory impairments. In addition, our data show that LDLr knockout (LDLr(-/-)) mice, regardless of Aβ treatment, displayed memory deficits and increased blood-brain barrier permeability. Nonetheless, LDLr(-/-) mice treated with Aβ(1-40) peptide presented increased acetylcholinesterase activity, astrogliosis, oxidative imbalance, and cell permeability within the hippocampus in comparison with Aβ(1-40)-treated C57BL/6 wild-type mice. Overall, the present study shows that the lack of LDLr increases the susceptibility to Aβ-induced neurotoxicity in mice providing new evidence about the crosslink between familial hypercholesterolemia and cognitive impairment.
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- 2014
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67. Protective effects of diphenyl diselenide in a mouse model of brain toxicity.
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Glaser V, Moritz B, Schmitz A, Dafré AL, Nazari EM, Rauh Müller YM, Feksa L, Straliottoa MR, de Bem AF, Farina M, da Rocha JB, and Latini A
- Subjects
- Animals, Antineoplastic Agents chemistry, Benzene Derivatives chemistry, Cell Line, Tumor, Cell Proliferation drug effects, Cell Survival drug effects, Cerebral Cortex metabolism, Cerebral Cortex pathology, Dose-Response Relationship, Drug, Drug Screening Assays, Antitumor, Male, Methylmercury Compounds chemistry, Methylmercury Compounds pharmacology, Mice, Neuroprotective Agents chemistry, Organoselenium Compounds chemistry, Structure-Activity Relationship, Antineoplastic Agents pharmacology, Benzene Derivatives pharmacology, Cerebral Cortex drug effects, Disease Models, Animal, Neuroprotective Agents pharmacology, Organoselenium Compounds pharmacology
- Abstract
Interest in organoselenide chemistry and biochemistry has increased in the past three decades, mainly due to their chemical and biological activities. Here, we investigated the protective effect of the organic selenium compound diphenyl diselenide (PhSe)2 (5 μmol/kg), in a mouse model of methylmercury (MeHg)-induced brain toxicity. Our group has previously demonstrated that the oral and repeated administration (21 days) of MeHg (40 mg/L) induced MeHg brain accumulation at toxic concentrations, and a pattern of severe cortical and cerebellar biochemical and behavioral. In order to assess neurotoxicity, the neurochemical parameters, namely, mitochondrial complexes I, II, II-III and IV, glutathione peroxidase (GPx) and glutathione reductase (GR) activities, the content of thiobarbituric acid-reactive substances (TBA-RS), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and brain-derived neurotrophic factor (BDNF), as well as, metal deposition were investigated in mouse cerebral cortex. Cortical neurotoxicity induced by brain MeHg deposition was characterized by the reduction of complexes I, II, and IV activities, reduction of GPx and increased GR activities, increased TBA-RS and 8-OHdG content, and reduced BDNF levels. The daily treatment with (PhSe)2 was able to counteract the inhibitory effect of MeHg on mitochondrial activities, the increased oxidative stress parameters, TBA-RS and 8-OHdG levels, and the reduction of BDNF content. The observed protective (PhSe)2 effect could be linked to its antioxidant properties and/or its ability to reduce MeHg deposition in brain, which was here histochemically corroborated. Altogether, these data indicate that (PhSe)2 could be consider as a neuroprotectant compound to be tested under neurotoxicity., (Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2013
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68. Diphenyl diselenide prevents cortico-cerebral mitochondrial dysfunction and oxidative stress induced by hypercholesterolemia in LDL receptor knockout mice.
- Author
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de Oliveira J, Moreira EL, Mancini G, Hort MA, Latini A, Ribeiro-do-Valle RM, Farina M, da Rocha JB, and de Bem AF
- Subjects
- Animals, Brain drug effects, Cerebral Cortex drug effects, Cholesterol blood, Cholesterol, Dietary administration & dosage, Electron Transport Complex I metabolism, Electron Transport Complex II metabolism, Glutathione metabolism, Glutathione Peroxidase metabolism, Male, Mice, Mice, Knockout, Mitochondria drug effects, Mitochondria metabolism, Oxidative Stress drug effects, Benzene Derivatives therapeutic use, Cerebral Cortex metabolism, Hypercholesterolemia physiopathology, Mitochondrial Diseases prevention & control, Neuroprotective Agents therapeutic use, Organoselenium Compounds therapeutic use, Receptors, LDL deficiency
- Abstract
Recent studies have indicated a causal link between high dietary cholesterol intake and brain oxidative stress. In particular, we have previously shown a positive correlation between elevated plasma cholesterol levels, cortico-cerebral oxidative stress and mitochondrial dysfunction in low density lipoprotein receptor knockout (LDLr(-/-)) mice, a mouse model of familial hypercholesterolemia. Here we show that the organoselenium compound diphenyl diselenide (PhSe)2 (1 mg/kg; o.g., once a day for 30 days) significantly blunted the cortico-cerebral oxidative stress and mitochondrial dysfunction induced by a hypercholesterolemic diet in LDLr(-/-) mice. (PhSe)2 effectively prevented the inhibition of complex I and II activities, significantly increased the reduced glutathione (GSH) content and reduced lipoperoxidation in the cerebral cortex of hypercholesterolemic LDLr(-/-) mice. Overall, (PhSe)2 may be a promising molecule to protect against hypercholesterolemia-induced effects on the central nervous system, in addition to its already demonstrated antiatherogenic effects.
- Published
- 2013
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69. Diphenyl diselenide supplementation reduces biochemical alterations associated with oxidative stress in rats fed with fructose and hydrochlorothiazide.
- Author
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Ribeiro MC, Avila DS, Schiar VP, Santos DB, Meinerz DF, Duarte MM, Monteiro R, Puntel R, de Bem AF, Hassan W, de Vargas Barbosa NB, and Rocha JB
- Subjects
- Animals, Catalase metabolism, Down-Regulation drug effects, Male, Rats, Rats, Wistar, Antioxidants pharmacology, Benzene Derivatives pharmacology, Diet, Dietary Supplements, Fructose metabolism, Hydrochlorothiazide metabolism, Organoselenium Compounds pharmacology, Oxidative Stress drug effects
- Abstract
The study evaluated whether a diet containing diphenyl diselenide (PhSe)2, a synthetic antioxidant, could reduce the biochemical alterations induced by chronic consumption of highly enriched fructose diet and/or hydrochlorothiazide (HCTZ). Rats were fed a control diet (CT) or a high fructose diet (HFD), supplemented with or not HCTZ (4.0g/kg) and/or (PhSe)2 (3ppm) for 18weeks. HFD intake increased significantly plasma glucose, fructosamine, triglycerides and cholesterol levels. (PhSe)2 supplementation significantly reduced triglycerides and cholesterol but could not restore them to control levels. The combination of HFD and HCTZ significantly altered plasma glucose, fructosamine, triglycerides and cholesterol levels which were not restore by (PhSe)2 supplementation. Lipid peroxidation, protein carbonyl formation, vitamin C level and catalase activity decreased after HFD, HCTZ or HFD plus HCTZ ingestion. Remarkably (PhSe)2 supplementation restored the oxidative stress parameters. HCTZ decreased renal superoxide dismutase (SOD) activity, which was restored to control levels by (PhSe)2. Furthermore, the association of HFD and HCTZ decreased plasma potassium levels and aggravated HCTZ-induced hypomagnesemia and hypertriglyceridemia. Here we provided evidence of the involvement of oxidative stress and metabolic disorders in a rat model of HFD associated or not with HTCZ. (PhSe)2 supplementation reduced the oxidative stress and this compound should be considered for the treatment of biochemical disturbances and oxidative stress in other animal models of metabolic disorders., (Copyright © 2013. Published by Elsevier Ireland Ltd.)
- Published
- 2013
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70. Diphenyl diselenide modulates oxLDL-induced cytotoxicity in macrophage by improving the redox signaling.
- Author
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Straliotto MR, Hort MA, Fiuza B, Rocha JB, Farina M, Chiabrando G, and de Bem AF
- Subjects
- Animals, Cells, Cultured, Humans, Lipoproteins, LDL toxicity, Matrix Metalloproteinase 1 metabolism, Mice, Mitochondria drug effects, Oxidation-Reduction drug effects, Protein Binding drug effects, Reactive Oxygen Species metabolism, Benzene Derivatives pharmacology, Macrophages drug effects, Organoselenium Compounds pharmacology, Signal Transduction drug effects
- Abstract
It has been reported that oxidized LDLs (oxLDL) are involved in the pathogenesis of atherosclerosis, and that macrophages as well as other cells of the arterial wall can oxidize LDL in vitro, depending on the balance between intracellular prooxidant generation and antioxidant defense efficiency. Because of their potential beneficial role in preventing atherosclerosis and other oxidative stress-related diseases, organoselenium compounds such as diphenyl diselenide (PhSe)2, are receiving increased attention. In the present work, we investigated the mechanisms underlying the protective effect exerted by (PhSe)2 on oxLDL-mediated effects in murine J774 macrophage-like cells. (PhSe)2 pretreatment reduced atherogenic signaling triggered by oxLDL in macrophages in vitro, namely: ROS generation, disturbance of NO homeostasis, activation of matrix metalloproteinase, foam cell formation, and mitochondrial dysfunction. Moreover, the redox signaling effects of (PhSe)2 presented herein were accompanied by a downregulation of NF-κB-binding activity. The relatively strong performance of (PhSe)2 makes it an ideal candidate for further, expanded trials as a new generation of antioxidants for preventing atherosclerotic lesion., (Copyright © 2013 Elsevier Masson SAS. All rights reserved.)
- Published
- 2013
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71. Atorvastatin prevents cell damage via modulation of oxidative stress, glutamate uptake and glutamine synthetase activity in hippocampal slices subjected to oxygen/glucose deprivation.
- Author
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Vandresen-Filho S, Martins WC, Bertoldo DB, Mancini G, Herculano BA, de Bem AF, and Tasca CI
- Subjects
- Animals, Atorvastatin, Cell Death drug effects, Glucose metabolism, Glutamic Acid drug effects, Male, Mice, Neurons drug effects, Neurons metabolism, Neuroprotective Agents pharmacology, Oxygen metabolism, Reactive Oxygen Species metabolism, Glutamate-Ammonia Ligase metabolism, Glutamic Acid metabolism, Heptanoic Acids pharmacology, Hippocampus drug effects, Oxidative Stress drug effects, Pyrroles pharmacology
- Abstract
Oxygen-glucose deprivation (OGD) in brain cells increases extracellular glutamate concentration leading to excitotoxicity. Glutamate uptake from the synaptic cleft is carried out by glutamate transporters, which are likely to be modulated by oxidative stress. Therefore, oxidative stress is associated with reduced activity of glutamate transporters and glutamine synthetase, thus increasing extracellular glutamate levels that may aggravate damage to brain cells. Atorvastatin, a cholesterol-lowering agent, has been shown to exert neuroprotective effects. The aim of this study was to investigate if in vivo atorvastatin treatment would have protective effects against hippocampal slices subjected to OGD, ex vivo. Atorvastatin pretreatment promoted increased cell viability after OGD and reoxygenation of hippocampal slices. Atorvastatin-induced neuroprotection may be related to diminished oxidative stress, since it prevented OGD-induced decrement of non-proteic thiols (NPSH) levels and increase in the production of reactive oxygen species (ROS). Atorvastatin pretreatment also prevented the OGD-induced decrease in glutamate uptake and glutamine synthetase activity, although it had no effect on OGD-induced excitatory aminoacids release. Addition of cholesterol before OGD and reoxygenation, abolished the protective effect of atorvastatin on cellular viability as well as on glutamate uptake and glutamine synthetase activity. Therefore, atorvastatin is capable of preventing OGD-induced cell death, an effect achieved due to modulation of glutamate uptake and glutamine synthetase activity, and associated with diminished oxidative stress. Additionally, atorvastatin effects were dependent on its action on cholesterol synthesis inhibition. Thus, atorvastatin might be a useful strategy in the prevention of glutamate exitotoxicity involved in brain injuries such as vascular disorders., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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72. Protective effect of diphenyl diselenide against peroxynitrite-mediated endothelial cell death: a comparison with ebselen.
- Author
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de Bem AF, Fiuza B, Calcerrada P, Brito PM, Peluffo G, Dinis TC, Trujillo M, Rocha JB, Radi R, and Almeida LM
- Subjects
- Analysis of Variance, Animals, Aorta cytology, Aorta drug effects, Aorta metabolism, Cattle, Cell Survival drug effects, Endothelial Cells cytology, Endothelial Cells metabolism, Glutathione metabolism, Glutathione Peroxidase metabolism, Isoindoles, NF-E2-Related Factor 2, Apoptosis drug effects, Azoles pharmacology, Benzene Derivatives pharmacology, Endothelial Cells drug effects, Organoselenium Compounds pharmacology, Peroxynitrous Acid toxicity, Protective Agents pharmacology
- Abstract
Excess production of superoxide (O₂(-)) and nitric oxide (NO) in blood vessel walls may occur early in atherogenesis leading to the formation of peroxynitrite, a strong oxidant and nitrating agent. This study was designed to determine the effect of diphenyl diselenide (PhSe)₂, a synthetic organoselenium compound, in comparison with ebselen, on peroxynitrite-mediated endothelial damage. Experimental results showed that pre-incubation of BAEC (24 h) with low concentrations of (PhSe)₂ (0.5 and 1 μM) protected the cells from peroxynitrite-dependent apoptosis and protein tyrosine nitration. The intracellular levels of GSH were almost completely depleted by peroxynitrite and, although the compounds did not restore its normal levels, (PhSe)₂ per se significantly increased GSH in a concentration-dependent manner. Moreover, (PhSe)₂, which was about two times more active as a GPx mimic than ebselen, induced a significantly higher increase in both cellular GPx expression and activity. Taking into account the kinetics of the reaction between peroxynitrite and (PhSe)₂, our data indicate that (PhSe)₂ protects BAEC against peroxynitrite-mediated cell damage not by a direct reaction, but rather by increasing cellular GPx expression as a consequence of enhanced nuclear translocation of Nrf-2, which together with the increase in intracellular GSH, may work catalytically to reduce peroxynitrite to nitrite., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2013
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73. Effects of lifestyle modifications on cognitive impairments in a mouse model of hypercholesterolemia.
- Author
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Moreira EL, Aguiar AS Jr, de Carvalho CR, Santos DB, de Oliveira J, de Bem AF, Xikota JC, Walz R, Farina M, and Prediger RD
- Subjects
- Animals, Anticholesteremic Agents pharmacology, Anticholesteremic Agents therapeutic use, Cognition Disorders etiology, Cognition Disorders therapy, Founder Effect, Hyperlipoproteinemia Type II complications, Hyperlipoproteinemia Type II drug therapy, Mice, Mice, Knockout, Motor Activity, Probucol pharmacology, Probucol therapeutic use, Receptors, LDL genetics, Social Environment, Cognition Disorders psychology, Hyperlipoproteinemia Type II psychology, Physical Conditioning, Animal
- Abstract
Epidemiological studies indicate that high midlife plasma cholesterol levels increases the risk of Alzheimer's disease. Moreover, middle-aged familial hypercholesterolemia (FH) subjects show a particularly high incidence of mild cognitive impairments (MCI). These evidence points to hypercholesterolemia as one of the modifiable risk factors focused on prevention/treatment of cognitive deterioration. The present study draws a comparison between pharmacological (lipid-lowering drug probucol) and non-pharmacological (voluntary running wheel, RW) approaches for the management of hypercholesterolemia and cognitive impairments associated with the low-density lipoprotein receptor-deficient (LDLr(-/-)) mice, a well-established rodent model of FH. We also investigated whether exposure to environmental enrichment (EE), a feasible option to increase physical activity in young mice cohort, from birth to adolescence (PN45) yields long-term behavioral changes in adult LDLr(-/-) mice (PN90). We observed that both probucol and RW significantly decreased total and non-HDL plasma cholesterol levels in LDLr(-/-) mice. Notably, only physical exercise mitigated the spatial memory deficits of LDLr(-/-) mice. In addition, we showed that exposure to EE from birth until the adolescence did not mitigate the spatial memory deficits of adult LDLr(-/-) mice in the object location task, although it induced persistent anxyolitic-like effects in the open field arena. Collectively, our results emphasize the advantages physical exercise, in comparison to lipid-lowering drugs, for the management of cognitive deficits associated with FH., (Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2013
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74. Disubstituted diaryl diselenides as potential atheroprotective compounds: Involvement of TrxR and GPx-like systems.
- Author
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Straliotto MR, de Oliveira J, Mancini G, Bainy AC, Latini A, Deobald AM, Rocha JB, and de Bem AF
- Subjects
- Animals, Atherosclerosis prevention & control, Glutathione metabolism, Humans, Lipoproteins, LDL metabolism, Mice, Oxidation-Reduction, Antioxidants pharmacology, Glutathione Peroxidase metabolism, Organoselenium Compounds pharmacology, Thioredoxin Reductase 1 metabolism
- Abstract
Oxidative modifications of low-density lipoproteins (LDLs) have a determinant role in atherogenesis and the study of agents that can modulate LDL oxidation is of pharmacological and therapeutic significance. Therefore, the aim of this study was to evaluate the antioxidant effect of the disubstituted diaryl diselenides, p-methoxyl-diphenyl diselenide (p-CH(3)O-C(6)H(4)Se)(2) (DM) and p-chloro-diphenyl diselenide (p-Cl-C(6)H(4)Se)(2) (DC), on Cu(2+)-induced LDL oxidation. Both compounds caused a dose-dependent inhibition of human serum and isolated LDL oxidation evidenced by the increasing of the lag phase of lipid peroxidation and decreased the lipid oxidation rate (V(max)). The protein moieties from isolated LDL were also protected from Cu(2+)-induced oxidation. Moreover, the disubstituted diaryl diselenides efficiently decreased the oxidized LDL (ox-LDL) induced foam cell formation in J774A.1 macrophage cells. Mechanistically, we have demonstrated that the antioxidant and antiatherogenic effects of DM and DC are related to formation of their selenol intermediates (RSeH) either by a direct reaction with endogenous thiols (GPx-like activity) or via their reduction by TrxR (using NADPH as electron donor). Considering the powerful effect of DM and DC against LDL-induced toxicity, they could be considered for developing of new therapeutic approaches to preventing and treating atherosclerosis and cardiovascular diseases., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
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75. Does methylmercury-induced hypercholesterolemia play a causal role in its neurotoxicity and cardiovascular disease?
- Author
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Moreira EL, de Oliveira J, Dutra MF, Santos DB, Gonçalves CA, Goldfeder EM, de Bem AF, Prediger RD, Aschner M, and Farina M
- Subjects
- Animals, Anticholesteremic Agents pharmacology, Behavior, Animal drug effects, Biomarkers blood, Cerebellum drug effects, Cerebellum metabolism, Cerebellum physiopathology, Cholesterol blood, Cholesterol, LDL blood, Glutathione Peroxidase metabolism, Hypercholesterolemia blood, Hypercholesterolemia genetics, Hypercholesterolemia prevention & control, Kidney drug effects, Kidney metabolism, Kidney pathology, Liver drug effects, Liver metabolism, Male, Mercury Poisoning, Nervous System pathology, Mercury Poisoning, Nervous System physiopathology, Mice, Mice, Inbred C57BL, Mice, Knockout, Motor Activity drug effects, Neuroglia drug effects, Neuroglia pathology, Probucol pharmacology, Receptors, LDL deficiency, Receptors, LDL genetics, Time Factors, Urea blood, Cardiovascular Diseases chemically induced, Hypercholesterolemia chemically induced, Mercury Poisoning, Nervous System etiology, Methylmercury Compounds toxicity, Water Pollutants, Chemical toxicity
- Abstract
Methylmercury (MeHg) is an environmental pollutant that biomagnifies throughout the aquatic food chain, thus representing a toxicological concern for humans subsiding on fish for their dietary intake. Although the developing brain is considered the critical target organ of MeHg toxicity, recent evidence indicates that the cardiovascular system may be the most sensitive in adults. However, data on the mechanisms mediating MeHg-induced cardiovascular toxicity are scarce. Based on the close relationship between cardiovascular disease and dyslipidemia, this study was designed to investigate the effects of long-term MeHg exposure on plasma lipid levels in mice, as well as their underlying mechanisms and potential relationships to MeHg-induced neurotoxicity. Our major finding was that long-term MeHg exposure induced dyslipidemia in rodents. Specifically, Swiss and C57BL/6 mice treated for 21 days with a drinking solution of MeHg (40 mg/l, ad libitum) diluted in tap water showed increased total and non-HDL plasma cholesterol levels. MeHg-induced hypercholesterolemia was also observed in low-density lipoprotein receptor knockout (LDLr⁻/⁻) mice, indicating that this effect was not related to decreased LDLr-mediated cholesterol transport from blood to other tissues. Although the hepatic synthesis of cholesterol was unchanged, significant signs of nephrotoxicity (glomerular shrinkage, tubular vacuolization, and changed urea levels) were observed in MeHg-exposed mice, indicating that the involvement of nephropathy in MeHg-induced lipid dyshomeostasis may not be ruled out. Notably, Probucol (a lipid-lowering drug) prevented the development of hypercholesterolemia when coadministered with MeHg. Finally, hypercholesterolemic LDLr⁻/⁻ mice were more susceptible to MeHg-induced cerebellar glial activation, suggesting that hypercholesterolemia in itself may pose a risk factor in MeHg-induced neurotoxicity. Overall, based on the strong and graded positive association between total as well as LDL cholesterol and risk of cardiovascular diseases, our data support the concept of MeHg-induced cardiovascular toxicity.
- Published
- 2012
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76. Cardioprotective effects of a proanthocyanidin-rich fraction from Croton celtidifolius Baill: focus on atherosclerosis.
- Author
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Hort MA, Straliotto MR, Duz MS, Netto PM, Souza CB, Schulz T, Horst H, Pizzolatti MG, de Bem AF, and Ribeiro-do-Valle RM
- Subjects
- Animals, Cells, Cultured, Cholesterol, LDL chemistry, Copper, Endothelial Cells drug effects, Mice, Mice, Knockout, Oxidation-Reduction, Oxidative Stress, Plant Bark chemistry, Plant Extracts chemistry, Proanthocyanidins chemistry, Receptors, LDL genetics, Receptors, LDL metabolism, Vasodilation drug effects, Coronary Artery Disease prevention & control, Croton chemistry, Plant Extracts pharmacology, Proanthocyanidins pharmacology
- Abstract
Proanthocyanidins are the most abundant polyphenols in human diets. Epidemiological studies have pointed to proanthocyanidins as promising molecules that could prevent the development of several coronary syndromes by inhibiting the atherogenic process. The present study was designed to investigate the antiatherogenic effects of a proanthocyanidin-rich fraction (PRF) obtained from Croton celtidifolius Baill (Euphorbiaceae) barks. In isolated human LDL, PRF caused a concentration-dependent inhibition of Cu2+-induced oxidative modifications, evidenced by the increasing of the lag phase of lipid peroxidation and decreasing in the oxidation rate (Vmax), moreover, the protein moieties from LDL were protected against Cu2+-induced oxidation. In human umbilical vein endothelial cells (HUVECs), PRF reduced the ROS production stimulated by oxidized LDL. Herein, we demonstrate that oral treatment with PRF improved endothelium-dependent vasorelaxation in hypercholesterolemic LDL receptor knockout mice (LDLr-/-), however, the fraction did not modify plasma lipids and atherosclerotic lesion size in this experimental model. Finally, our results showed for the first time that PRF prevent isolated LDL oxidation, decrease oxidative stress in endothelial cells and improve endothelial function in mice., (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Published
- 2012
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77. Diphenyl diselenide-modulation of macrophage activation: down-regulation of classical and alternative activation markers.
- Author
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Rupil LL, de Bem AF, and Roth GA
- Subjects
- Animals, Antigen Presentation drug effects, Cell Line, Dexamethasone immunology, Down-Regulation, Interleukin-10 metabolism, Lectins, C-Type metabolism, Lipopolysaccharides immunology, Macrophages, Peritoneal immunology, Mannose Receptor, Mannose-Binding Lectins metabolism, Mice, Nitric Oxide metabolism, Nitric Oxide Synthase Type II metabolism, Reactive Oxygen Species metabolism, Receptors, Cell Surface metabolism, Benzene Derivatives pharmacology, Macrophage Activation drug effects, Macrophages, Peritoneal drug effects, Organoselenium Compounds pharmacology
- Abstract
Diphenyl diselenide (PhSe)(2), a simple organoselenium compound, possesses interesting pharmacological properties that are under extensive research. As macrophages respond to microenvironmental stimuli and can display activities engaged in the initiation and the resolution of inflammation, in the present report we describe the ability of (PhSe)(2) to modulate the macrophage activation. Our data indicate that (PhSe)(2) could inhibit the NO production in a dose-dependent fashion in peritoneal macrophages activated by LPS or treated with vehicle alone. We could demonstrate that this effect correlated with a reduction in the expression of the inducible NO synthase in (PhSe)(2)-treated cells. Furthermore, (PhSe)(2) suppressed the production of reactive oxygen species, diminished the activity of the arginase enzyme, and the accumulation of nitrotyrosine modified proteins in LPS-stimulated macrophages. This compound also diminished the antigen presentation capacity of classically activated macrophages, as it reduced MHCII and CD86 expression. In addition, (PhSe)(2) modulated the alternative activation phenotype of macrophages. Dexamethasone-activated macrophages presented higher production of IL-10 and CD206, which were both down-regulated by the addition of (PhSe)(2). These results suggest that (PhSe)(2) possesses antioxidant and anti-inflammatory activities in classically-activated macrophages. We could demonstrate that (PhSe)(2) can be also utilized to modulate the alternative activation phenotype of macrophages.
- Published
- 2012
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78. Positive correlation between elevated plasma cholesterol levels and cognitive impairments in LDL receptor knockout mice: relevance of cortico-cerebral mitochondrial dysfunction and oxidative stress.
- Author
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de Oliveira J, Hort MA, Moreira EL, Glaser V, Ribeiro-do-Valle RM, Prediger RD, Farina M, Latini A, and de Bem AF
- Subjects
- Alzheimer Disease metabolism, Alzheimer Disease physiopathology, Animals, Brain metabolism, Cholesterol blood, Cognition Disorders genetics, Cognition Disorders metabolism, Disease Models, Animal, Glutathione Peroxidase analysis, Glutathione Peroxidase metabolism, Glutathione Reductase analysis, Glutathione Reductase metabolism, Hypercholesterolemia metabolism, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Receptors, LDL deficiency, Brain physiopathology, Cognition Disorders physiopathology, Hypercholesterolemia physiopathology, Oxidative Stress physiology
- Abstract
Convergent epidemiological, clinical, and experimental findings indicate that hypercholesterolemia contributes to the onset of Alzheimer's disease (AD)-like dementia, but the exact underlying mechanisms remains unknown. In this study, we evaluated the cognitive performance of mice submitted to a model of hypercholesterolemia, as well as its relationship with mitochondrial dysfunction and oxidative stress, two key events involved in AD pathogenesis. Wild-type C57bl/6 or low density lipoprotein receptor (LDLr)-deficient mice were fed with either standard or cholesterol-enriched diet for a 4-week period and tested for spatial learning and memory in the object location task. LDLr⁻/⁻ mice displayed spatial learning and memory impairments regardless of diet. Moreover, LDLr⁻/⁻ mice fed cholesterol-enriched diet presented a significant decrease in the mitochondrial complexes I and II activities in the cerebral cortex, which were negatively correlated with respective blood cholesterol levels. Additionally, hypercholesterolemic LDLr⁻/⁻ mice presented a significant decrease in glutathione levels, about 40% increase in the thiobarbituric acid-reactive substances levels, as well as an imbalance between the peroxide-removing-related enzymes glutathione peroxidase/glutathione reductase activities in the cerebral cortex. These findings indicate a significant relationship between hypercholesterolemia, cognitive impairment, and cortico-cerebral mitochondrial dysfunctional/oxidative stress. Because of the involvement of such alterations in AD patients, our data render this mouse model of hypercholesterolemia a useful approach to comprehend the molecular events mediating AD pathogenesis., (Copyright © 2011 IBRO. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2011
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79. Effects of K074 and pralidoxime on antioxidant and acetylcholinesterase response in malathion-poisoned mice.
- Author
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dos Santos AA, dos Santos DB, Ribeiro RP, Colle D, Peres KC, Hermes J, Barbosa AM, Dafré AL, de Bem AF, Kuca K, and Farina M
- Subjects
- Acetylcholinesterase blood, Animals, Antidotes toxicity, Brain enzymology, Butanes toxicity, Catalase metabolism, Cholinesterase Reactivators toxicity, GPI-Linked Proteins blood, GPI-Linked Proteins metabolism, Glutathione Peroxidase metabolism, Glutathione Reductase metabolism, Lethal Dose 50, Lipid Peroxidation drug effects, Male, Mice, Oxidative Stress drug effects, Oximes toxicity, Poisoning drug therapy, Poisoning enzymology, Pralidoxime Compounds toxicity, Pyridinium Compounds toxicity, Time Factors, Acetylcholinesterase metabolism, Antidotes pharmacology, Antioxidants metabolism, Brain drug effects, Butanes pharmacology, Cholinesterase Inhibitors poisoning, Cholinesterase Reactivators pharmacology, Insecticides poisoning, Malathion poisoning, Oximes pharmacology, Pralidoxime Compounds pharmacology, Pyridinium Compounds pharmacology
- Abstract
The organophosphorus (OP) pesticide malathion is a highly neurotoxic compound and its toxicity is primarily caused by the inhibition of acetylcholinesterase (AChE), leading to cholinergic syndrome. Although oximes have been used as potential antidotal treatments in malathion poisoning because of their potential capability to reactivate the inhibited enzyme, the clinical experience with the clinically available oximes (e.g. pralidoxime) is disappointing and their routine use has been questioned. In the present study, we investigated the potency of pralidoxime and K074 in reactivating AChE after acute exposure to malathion, as well as in preventing malathion-induced changes in oxidative-stress related parameters in mice. Malathion (1.25 g/kg, s.c.) induced a significant decrease in cortico-cerebral, hippocampal and blood AChE activities at 24h after exposure. Oxime treatments (1/4 of LD(50), i.m., 6h after malathion poisoning) showed that pralidoxime significantly reversed malathion-induced blood AChE inhibition, although no significant effects were observed after K074 treatment. Interestingly, both oximes tested were unable to reactivate the cortico-cerebral and hippocampal enzymes after intramuscular or intracerebroventricular injection (1/4 of LD(50), 6h after malathion poisoning). Biochemical parameters related to oxidative stress (cerebro-cortical and hippocampal glutathione peroxidase, glutathione reductase and catalase activities, as well as lipid peroxidation) were not affected in animals treated with malathion, oximes or atropine alone. However, pralidoxime and K074, administered intramuscularly 6h after malathion poisoning, were able to increase the endogenous activities of these antioxidant enzymes in the prefrontal cortex and hippocampus. Taken together, the results presented herein showed that pralidoxime (the most common clinically used oxime) and the recently developed oxime K074, administered 6h after malathion poisoning, were unable to reactivate the inhibited AChE in mouse prefrontal cortex and hippocampus. However, only pralidoxime significantly reversed the blood AChE inhibition induced by malathion poisoning. This indicates that peripheral and central AChE activities are not necessarily correlated after the treatment of OP compounds and/or oximes, which should be taken into account in the diagnosis and management of OP-exposed humans. In addition, considering that the available treatments to malathion poisoning appear to be ineffective, the present study reinforce the need to search for potential new AChE reactivators able to efficiently reactivate the brain and blood AChEs after malathion poisoning., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2011
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80. Effect of diphenyl diselenide on the development of experimental autoimmune encephalomyelitis.
- Author
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Chanaday NL, de Bem AF, and Roth GA
- Subjects
- Animals, Antioxidants pharmacology, Autoimmunity immunology, Benzene Derivatives pharmacology, Cell Proliferation drug effects, Central Nervous System metabolism, Demyelinating Diseases drug therapy, Encephalomyelitis, Autoimmune, Experimental immunology, Encephalomyelitis, Autoimmune, Experimental metabolism, Immunity, Humoral drug effects, Myelin Sheath, Organoselenium Compounds pharmacology, Oxidative Stress physiology, Rats, T-Lymphocytes drug effects, T-Lymphocytes immunology, Antioxidants therapeutic use, Benzene Derivatives therapeutic use, Encephalomyelitis, Autoimmune, Experimental drug therapy, Organoselenium Compounds therapeutic use
- Abstract
Experimental autoimmune encephalomyelitis (EAE) is a T cell-mediated inflammatory and demyelinating disease of the central nervous system with clinical and pathological similarities with multiple sclerosis. The oxidative stress is one of the major mediators of demyelination and axonal damage in both, multiple sclerosis and EAE. Therefore, several studies are being performed to assess whether treatment with antioxidants prevents the progression of these diseases. Some organic forms of selenium that exhibit glutathione peroxidase-like activity have become good candidates for disease prevention and therapy since they catalytically remove oxidative stressors. Particularly, diphenyl diselenide ((PhSe)(2)) exerts antioxidant activity and has neuroprotective effects in several systems. The aim of the present study was to prove the therapeutic activity of (PhSe)(2) on the development of EAE. Intraperitoneally administered (PhSe)(2) (1-25 μmoles/kg body weight/day) reduced the incidence of the disease but was also deleterious for the animals. Conversely, (PhSe)(2) given orally (80 μmoles/kg body weight/day) produced a significant inhibition of EAE without any toxic effect. In addition, there was a reduction of the characteristic histological alterations and a diminished in vivo and in vitro T-cell response against the encephalitogenic myelin basic protein. These results show an effective suppression of the autoimmune response that could be the base for future developments of successful antioxidants therapies in EAE as well as in multiple sclerosis., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
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81. Differential effects of insulin on peripheral diabetes-related changes in mitochondrial bioenergetics: involvement of advanced glycosylated end products.
- Author
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Remor AP, de Matos FJ, Ghisoni K, da Silva TL, Eidt G, Búrigo M, de Bem AF, Silveira PC, de León A, Sanchez MC, Hohl A, Glaser V, Gonçalves CA, Quincozes-Santos A, Borba Rosa R, and Latini A
- Subjects
- Adult, Aged, Aged, 80 and over, Animals, Antibiotics, Antineoplastic toxicity, Blood Glucose metabolism, Case-Control Studies, Cells, Cultured, Electron Transport, Fibroblasts cytology, Fibroblasts metabolism, Heart physiology, Humans, Hyperglycemia chemically induced, Immunoenzyme Techniques, Male, Middle Aged, Mitochondria pathology, Muscle, Skeletal cytology, Muscle, Skeletal metabolism, Oxidative Stress, Oxygen Consumption, Pyruvaldehyde metabolism, Rats, Rats, Wistar, Reactive Oxygen Species metabolism, Skin cytology, Skin metabolism, Streptozocin toxicity, Diabetes Mellitus physiopathology, Energy Metabolism, Glycation End Products, Advanced metabolism, Hyperglycemia physiopathology, Hypoglycemic Agents pharmacology, Insulin pharmacology, Mitochondria metabolism
- Abstract
Large scale clinical trials have demonstrated that an intensive antihyperglycemic treatment in diabetes mellitus (DM) in individuals reduces the incidence of micro- and macrovascular complications, e.g. nephropathy, retinopathy, DM-accelerated atherosclerosis, myocardial infarction, or limb amputations. Here, we investigated the effect of short- and long-term insulin administration on mitochondrial function in peripheral tissues of streptozotocin (STZ)-induced hyperglycemic rats. In addition, the in vitro effect of methylglyoxal (MG), advanced glycation end products (AGEs) and human diabetic plasma on mitochondrial activity was investigated in skeletal muscle and liver mitochondria and in rat skin primary fibroblasts. Hyperglycemic STZ rats showed tissue-specific patterns of energy deficiency, evidenced by reduced activities of complexes I, II and/or IV after 30 days of hyperglycemia in heart, skeletal muscle and liver; moreover, cardiac tissue was found to be the most sensitive to the diabetic condition, since energy metabolism was impaired after 10 days of the hyperglycemia. Insulin-induced tight glycemic control was effective in protecting against the hyperglycemia-induced inhibition of mitochondrial enzyme activities. Furthermore, the long-term hormone replacement (30 days) also increased these activities in kidney from STZ-treated animals, where the hyperglycemic state did not modify the electron transport activity. Results from in vitro experiments indicate that mitochondrial impairment could result from oxidative stress-induced accumulation of MG and/or AGEs. Further investigations demonstrated that human plasma AGE accumulation elicits reduced mitochondrial function in skin fibroblast. These data suggest that persistent hyperglycemia results in tissue-specific patterns of energy deficiency and that early and continuous insulin therapy is necessary to maintain proper mitochondrial metabolism., (2011 Elsevier B.V. All rights reserved.)
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- 2011
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82. Molecular aspects involved in swimming exercise training reducing anhedonia in a rat model of depression.
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Sigwalt AR, Budde H, Helmich I, Glaser V, Ghisoni K, Lanza S, Cadore EL, Lhullier FL, de Bem AF, Hohl A, de Matos FJ, de Oliveira PA, Prediger RD, Guglielmo LG, and Latini A
- Subjects
- Animals, Brain-Derived Neurotrophic Factor biosynthesis, Depression complications, Dexamethasone toxicity, Disease Models, Animal, Gene Expression physiology, Glucocorticoids toxicity, Hippocampus metabolism, Hypothalamo-Hypophyseal System metabolism, Interleukin-10 biosynthesis, Male, Pituitary-Adrenal System metabolism, Rats, Rats, Wistar, Real-Time Polymerase Chain Reaction, Swimming, Anhedonia physiology, Depression rehabilitation, Physical Conditioning, Animal methods
- Abstract
Patients suffering from depression frequently display hyperactivity of the hypothalamic-pituitary-adrenal axis (HPA) resulting in elevated cortisol levels. One main symptom of this condition is anhedonia. There is evidence that exercise training can be used as a rehabilitative intervention in the treatment of depressive disorders. In this scenario, the aim of the present study was to assess the effect of an aerobic exercise training protocol on the depressive-like behavior, anhedonia, induced by repeated dexamethasone administration. The study was carried out on adult male Wistar rats randomly divided into four groups: the "control group" (C), "exercise group" (E), "dexamethasone group" (D) and the "dexamethasone plus exercise group" (DE). The exercise training consisted of swimming (1 h/d, 5 d/wk) for 3 weeks, with an overload of 5% of the rat body weight. Every day rats were injected with either dexamethasone (D/DE) or saline solution (C/E). Proper positive controls, using fluoxetine, were run in parallel. Decreased blood corticosterone levels, reduced adrenal cholesterol synthesis and adrenal weight (HPA disruption), reduced preference for sucrose consumption and increased immobility time (depressive-like behavior), marked hippocampal DNA oxidation, increased IL-10 and total brain-derived neurotrophic factor (BDNF; pro-plus mature-forms) and a severe loss of body mass characterized the dexamethasone-treated animals. Besides increasing testosterone blood concentrations, the swim training protected depressive rats from the anhedonic state, following the same profile as fluoxetine, and also from the dexamethasone-induced impaired neurochemistry. The data indicate that physical exercise could be a useful tool in preventing and treating depressive disorders., (Copyright © 2011 IBRO. Published by Elsevier Ltd. All rights reserved.)
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- 2011
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83. Diphenyl diselenide effectively reduces atherosclerotic lesions in LDLr -/- mice by attenuation of oxidative stress and inflammation.
- Author
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Hort MA, Straliotto MR, Netto PM, da Rocha JB, de Bem AF, and Ribeiro-do-Valle RM
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- Animals, Antioxidants pharmacology, Antioxidants therapeutic use, Atherosclerosis genetics, Atherosclerosis pathology, Benzene Derivatives pharmacology, Cells, Cultured, Inflammation Mediators pharmacology, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Organoselenium Compounds pharmacology, Oxidative Stress genetics, Random Allocation, Receptors, LDL genetics, Atherosclerosis drug therapy, Benzene Derivatives therapeutic use, Inflammation Mediators therapeutic use, Organoselenium Compounds therapeutic use, Oxidative Stress drug effects, Receptors, LDL deficiency
- Abstract
Glutathione peroxidase (GPx) plays an important role in the antioxidant defense of the vascular wall, and its deficiency has been implicated in the development of atherosclerotic lesions. This study analyzed the potential of diphenyl diselenide (DD), a simple organoselenium compound with GPx-like activity, to reduce atherosclerosis. Herein, we demonstrate that oral treatment with low doses of DD potently reduced the formation of atherosclerotic lesion in hypercholesterolemic low-density lipoprotein (LDL) receptor knockout (LDLr -/-) mice. This reduction was accompanied by significantly improved endothelium-dependent vasorelaxation, lower nitrotyrosine and malondialdehyde levels, decrease in vessel-wall infiltration by inflammatory cells, and prevention of upregulation of the proatherogenic monocyte chemoattractant protein-1. Studies in J774 macrophage-like cells show that DD significantly decreased oxLDL-induced formation of foam cells and the generation of reactive oxygen species and inflammatory mediators. Our results reveal the antiatherogenic actions of DD by modulating intracellular signaling pathways related to antioxidant and anti-inflammatory responses.
- Published
- 2011
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84. The intranasal administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP): a new rodent model to test palliative and neuroprotective agents for Parkinson's disease.
- Author
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Prediger RD, Aguiar AS Jr, Moreira EL, Matheus FC, Castro AA, Walz R, De Bem AF, Latini A, Tasca CI, Farina M, and Raisman-Vozari R
- Subjects
- Administration, Intranasal, Animals, Behavior, Animal drug effects, Behavior, Animal physiology, Biogenic Monoamines metabolism, Brain drug effects, Brain metabolism, Brain physiopathology, Humans, MPTP Poisoning chemically induced, MPTP Poisoning psychology, Neuroprotective Agents pharmacology, 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine administration & dosage, Disease Models, Animal, Drug Evaluation, Preclinical methods, MPTP Poisoning physiopathology, Neuroprotective Agents therapeutic use, Parkinson Disease drug therapy, Parkinson Disease physiopathology
- Abstract
Parkinson's disease (PD) is the second most common neurodegenerative disorder affecting approximately 1% of the population older than 60 years. Classically, PD is considered to be a motor system disease and its diagnosis is based on the presence of a set of cardinal motor signs that are consequence of a pronounced death of dopaminergic neurons in the substantia nigra pars compacta (SNc). Nowadays there is considerable evidence showing that non-dopaminergic degeneration also occurs in other brain areas which seems to be responsible for the deficits in olfactory, emotional and memory functions that precede the classical motor symptoms in PD. Dopamine-replacement therapy has dominated the treatment of PD and although the currently approved antiparkinsonian agents offer effective relief of the motor deficits, they have not been found to alleviate the non-motor features as well as the underlying dopaminergic neuron degeneration and thus drug efficacy is gradually lost. Another major limitation of chronic dopaminergic therapy is the numerous adverse effects such as dyskinesias, psychosis and behavioral disturbance. The development of new therapies in PD depends on the existence of representative animal models to facilitate the evaluation of new pharmacological agents before they are applied in clinical trials. We have recently proposed a new experimental model of PD consisting of a single intranasal (i.n.) administration of the proneurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 1 mg/nostril) in rodents. Our findings demonstrated that rats and mice treated intranasally with MPTP suffer impairments in olfactory, cognitive, emotional and motor functions conceivably analogous to those observed during different stages of PD. Such infusion causes time-dependent loss of tyrosine hydroxylase in the olfactory bulb and SNc, resulting in significant dopamine depletion in different brain areas. We have also identified some pathogenic mechanisms possibly involved in the neurodegeneration induced by i.n. administration of MPTP including mitochondrial dysfunction, oxidative stress, activation of apoptotic cell death mechanisms and glutamatergic excitotoxicity. Therefore, the present review attempts to provide a comprehensive picture of the i.n. MPTP model and to highlight recent findings from our group showing its potential as a valuable rodent model for testing novel drugs that may provide alternative or adjunctive treatment for both motor and non-motor symptoms relief with a reduced side-effect profile as well as the discovery of compounds to modify the course of PD.
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- 2011
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85. High-intensity physical exercise disrupts implicit memory in mice: involvement of the striatal glutathione antioxidant system and intracellular signaling.
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Aguiar AS Jr, Boemer G, Rial D, Cordova FM, Mancini G, Walz R, de Bem AF, Latini A, Leal RB, Pinho RA, and Prediger RD
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- Adaptation, Physiological physiology, Analysis of Variance, Animals, Disease Models, Animal, Electron Transport Chain Complex Proteins metabolism, Exercise Test, Fear physiology, Freezing Reaction, Cataleptic physiology, Glutathione Peroxidase metabolism, Glutathione Reductase metabolism, Lactic Acid blood, Male, Maze Learning physiology, Memory Disorders blood, Mice, Motor Activity physiology, Muscle, Skeletal physiopathology, Corpus Striatum metabolism, Glutathione metabolism, Memory Disorders etiology, Memory Disorders pathology, Physical Conditioning, Animal adverse effects, Signal Transduction physiology
- Abstract
Physical exercise is a widely accepted behavioral strategy to enhance overall health, including mental function. However, there is controversial evidence showing brain mitochondrial dysfunction, oxidative damage and decreased neurotrophin levels after high-intensity exercise, which presumably worsens cognitive performance. Here we investigated learning and memory performance dependent on different brain regions, glutathione antioxidant system, and extracellular signal-regulated protein kinase 1/2 (ERK1/2), serine/threonine protein kinase (AKT), cAMP response element binding (CREB) and dopamine- and cyclic AMP-regulated phosphoprotein (DARPP)-32 signaling in adult Swiss mice submitted to 9 weeks of high-intensity exercise. The exercise did not alter the animals' performance in the reference and working memory versions of the water maze task. On the other hand, we observed a significant impairment in the procedural memory (an implicit memory that depends on basal ganglia) accompanied by a reduced antioxidant capacity and ERK1/2 and CREB signaling in this region. In addition, we found increased striatal DARPP-32-Thr-75 phosphorylation in trained mice. These findings indicate an increased vulnerability of the striatum to high-intensity exercise associated with the disruption of implicit memory in mice and accompanied by alteration of signaling proteins involved in the plasticity of this brain structure., (Copyright © 2010 IBRO. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2010
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86. Proanthocyanidin-rich fraction from Croton celtidifolius Baill confers neuroprotection in the intranasal 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine rat model of Parkinson's disease.
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Moreira EL, Rial D, Aguiar AS Jr, Figueiredo CP, Siqueira JM, DalBó S, Horst H, de Oliveira J, Mancini G, dos Santos TS, Villarinho JG, Pinheiro FV, Marino-Neto J, Ferreira J, De Bem AF, Latini A, Pizzolatti MG, Ribeiro-do-Valle RM, and Prediger RD
- Subjects
- Administration, Intranasal, Animals, Disease Models, Animal, Male, Neuroprotective Agents administration & dosage, Plant Extracts administration & dosage, Proanthocyanidins therapeutic use, Rats, Rats, Wistar, Croton chemistry, Neuroprotective Agents pharmacology, Parkinsonian Disorders drug therapy, Plant Extracts pharmacology, Proanthocyanidins pharmacology
- Abstract
We have recently demonstrated that rodents treated intranasally with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) suffered impairments in olfactory, cognitive and motor functions associated with time-dependent disruption of dopaminergic neurotransmission in different brain structures conceivably analogous to those observed during different stages of Parkinson's disease (PD). On the other hand, the proanthocyanidin-rich fraction (PRF) obtained from the bark of Croton celtidifolius Baill (Euphorbiaceae), a tree frequently found in the Atlantic forest in south Brazil, has been described to have several neurobiological activities including antioxidant and anti-inflammatory properties, which may be of interest in the treatment of PD. The present data indicated that the pretreatment with PRF (10 mg/kg, i.p.) during five consecutive days was able to prevent mitochondrial complex-I inhibition in the striatum and olfactory bulb, as well as a decrease of the enzyme tyrosine hydroxylase expression in the olfactory bulb and substantia nigra of rats infused with a single intranasal administration of MPTP (1 mg/nostril). Moreover, pretreatment with PRF was found to attenuate the short-term social memory deficits, depressive-like behavior and reduction of locomotor activity observed at different periods after intranasal MPTP administration in rats. Altogether, the present findings provide strong evidence that PRF from C. celtidifolius may represent a promising therapeutic tool in PD, thus being able to prevent both motor and non-motor early symptoms of PD, together with its neuroprotective potential.
- Published
- 2010
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87. Atorvastatin prevents hippocampal cell death, neuroinflammation and oxidative stress following amyloid-β(1-40) administration in mice: evidence for dissociation between cognitive deficits and neuronal damage.
- Author
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Piermartiri TC, Figueiredo CP, Rial D, Duarte FS, Bezerra SC, Mancini G, de Bem AF, Prediger RD, and Tasca CI
- Subjects
- Amino Acid Transport System X-AG metabolism, Analysis of Variance, Animals, Animals, Newborn, Astrocytes drug effects, Atorvastatin, Cell Death drug effects, Cyclooxygenase 2 metabolism, Fluoresceins, Gene Expression Regulation drug effects, Glutamic Acid metabolism, Glutathione metabolism, Glutathione Peroxidase metabolism, Glutathione Reductase metabolism, Humans, In Vitro Techniques, Learning Disabilities chemically induced, Learning Disabilities drug therapy, Male, Maze Learning drug effects, Memory Disorders chemically induced, Memory Disorders drug therapy, Mice, Nerve Tissue Proteins metabolism, Organic Chemicals, Propidium, Tritium metabolism, Amyloid beta-Peptides toxicity, Encephalitis chemically induced, Encephalitis pathology, Encephalitis prevention & control, Heptanoic Acids pharmacology, Hippocampus pathology, Hydroxymethylglutaryl-CoA Reductase Inhibitors pharmacology, Neurons drug effects, Oxidative Stress drug effects, Peptide Fragments toxicity, Pyrroles pharmacology
- Abstract
The accumulation of amyloid-beta (Aβ) peptides in the brain of human and rodents has been associated with the activation of glial cells, neuroinflammatory and oxidative responses, and cognitive deficits. These oxidative changes leave glutamate transporters more vulnerable and may result in reduction of their functions, resulting in excitotoxic damage. Herein, we evaluated the effects of atorvastatin, a HMG-CoA reductase inhibitor, in molecular and behavioral alterations induced by a single intracerebroventricular injection of aggregated Aβ(1-40) (400 pmol) in mice. An increased glial fibrillar acidic protein (GFAP) expression and cyclooxygenase-2 (COX-2) levels, as well as increased lipid peroxidation and impairment in the glutathione antioxidant system and cell degeneration was found in the hippocampus of Aβ(1-40)-treated mice. Aβ(1-40) also induced a marked decrease in glutamatergic transporters (GLAST and GLT-1) expression and in l-[³H] glutamate uptake in mice hippocampus, in addition to spatial learning and memory deficits. Atorvastatin (10 mg/kg/day v.o.) was administered after Aβ(1-40) injection and through seven consecutive days. Atorvastatin treatment was neuroprotective against cell degeneration induced by Aβ(1-40), reducing inflammatory and oxidative responses and increasing the expression of glutamatergic transporters. On the other hand, atorvastatin did not reverse the cognitive impairments and failed to alter the hippocampal glutamate uptake in Aβ(1-40)-treated mice. These results reinforce and extend the notion of the potential neuroprotective action of atorvastatin against the neuronal toxicity induced by Aβ(1-40). In addition, the present findings suggest that the spatial learning and memory deficits induced by Aβ peptides in rodents may not be entirely related to neuronal damage., (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Published
- 2010
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88. Acute exposure of rabbits to diphenyl diselenide: a toxicological evaluation.
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Straliotto MR, Mancini G, de Oliveira J, Nazari EM, Müller YM, Dafre A, Ortiz S, Silva EL, Farina M, Latini A, Rocha JB, and de Bem AF
- Subjects
- Animals, Brain drug effects, Creatinine blood, Creatinine metabolism, Female, Glutathione Peroxidase metabolism, Heart drug effects, Kidney drug effects, Liver drug effects, Male, Muscles drug effects, Oxidative Stress, Porphobilinogen Synthase metabolism, Rabbits, Thiobarbituric Acid Reactive Substances metabolism, Toxicity Tests, Acute, Antioxidants pharmacology, Antioxidants toxicity, Benzene Derivatives metabolism, Benzene Derivatives toxicity, Drug Evaluation, Preclinical, Organoselenium Compounds metabolism, Organoselenium Compounds toxicity
- Abstract
The simple organoselenium compound diphenyl diselenide (PhSe)(2) is a promising new pharmacological agent. However, few toxicological evaluations of this molecule have been reported. We evaluated the effects of acute administration of (PhSe)(2) on toxicological parameters in rabbits. Adult New Zealand rabbits were exposed to (PhSe)(2) (5-500 micromol kg(-1) , intraperitoneally) once a day for 5 days. Exposure to 500 micromol kg(-1) caused 85% mortality. Exposure to 50 micromol kg(-1) of (PhSe)(2) increased the glutathione levels in the hippocampus, kidney, heart, muscle and blood, whereas lipoperoxidation (TBARS) decreased in the cerebellum and kidney after exposure to 5 micromol kg(-1) . The activity of glutathione peroxidase increased in the heart and muscle of rabbits treated with 50 micromol kg(-1) of (PhSe)(2) and glutathione reductase activity was reduced in the cerebellum, cerebral cortex and kidney. Treatment with (PhSe)(2) reduced the activity of δ-aminolevulinate dehydratase in the hippocampus and increased this activity in the heart, but did not alter the activity of complexes I and II of the respiratory chain in the liver and brain. Hepatic and renal biochemical and histological parameters were not modified by (PhSe)(2) and apoptosis was not detected in these tissues; however, the hepatic cells tended to accumulate fat vacuoles. These results indicated that acute toxicology to (PhSe)(2) in rabbit is dependent on the dose, which should motivate further experiments on the therapeutic properties of this compound., (2010 John Wiley & Sons, Ltd.)
- Published
- 2010
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89. Effects of inorganic selenium administration in methylmercury-induced neurotoxicity in mouse cerebral cortex.
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Glaser V, Nazari EM, Müller YM, Feksa L, Wannmacher CM, Rocha JB, de Bem AF, Farina M, and Latini A
- Subjects
- Animals, Cerebral Cortex metabolism, Electron Transport drug effects, Male, Mice, Oxidative Stress drug effects, Sodium Selenite pharmacology, Cerebral Cortex drug effects, Methylmercury Compounds toxicity, Neurotoxicity Syndromes metabolism, Sodium Selenite administration & dosage
- Abstract
Selenium can counteract methylmercury (MeHg) neurotoxicity. However, data about the neuroprotective effects of sodium selenite (Na(2)SeO(3)) on the activity of mitochondrial complexes and creatine kinase (mtCK) are scarce. Therefore, this study investigated the effects of the chronic exposure to Na(2)SeO(3) on brain energy metabolism and oxidative stress parameters in MeHg-poisoned mice. Adult male mice were orally treated with MeHg (40 mg L(-1) in drinking water, ad libitum) during 21 days and simultaneously administrated with daily subcutaneous injections of Na(2)SeO(3) (5 μmol kg(-1)), a potential neuroprotectant. Mitochondrial complexes I to IV and mtCK activities were measured in cerebral cortex mitochondria. The cerebro-cortical tissue was also used to evaluate the antioxidant enzymes glutathione peroxidase (GPx) and glutathione reductase (GR) activities, as well as lipid peroxidation. Metal deposition was followed autometalographically (AMG). Na(2)SeO(3) partially prevented MeHg-induced inhibition of complexes II-III, IV and mtCK activities; however, it was unable to prevent MeHg-induced complex I and II inhibition. MeHg increased lipid peroxidation, GR activity and decreased GPx activity in the cerebral cortex; however, Na(2)SeO(3) did not modify such events. Furthermore, Na(2)SeO(3)per se inhibited complexes I, II-III and IV and mtCK activities and increased GPx and GR activities and lipid peroxidation. These data show that inorganic selenium was ineffective in preventing most of the MeHg-induced brain biochemical alterations. However, the most prominent finding was the selenium-induced reduction of cells labelled for metal deposition. Although, the literature supports the beneficial effects of selenium against mercury toxicity, the toxic effects elicited by Na(2)SeO(3), alone or in combination with mercury, should be considered when this compound is proposed as a potential protective therapy for MeHg poisoning., (Copyright © 2010 ISDN. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2010
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90. In vitro reactivating effects of standard and newly developed oximes on malaoxon-inhibited mouse brain acetylcholinesterase.
- Author
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dos Santos AA, dos Santos DB, Dafre AL, de Bem AF, Souza DO, da Rocha JB, Kuca K, and Farina M
- Subjects
- Acetylcholinesterase metabolism, Animals, Brain enzymology, Butanes pharmacology, Enzyme Repression drug effects, Malathion toxicity, Male, Mice, Obidoxime Chloride pharmacology, Pralidoxime Compounds pharmacology, Pyridinium Compounds pharmacology, Trimedoxime pharmacology, Antidotes pharmacology, Brain drug effects, Cholinesterase Inhibitors toxicity, Cholinesterase Reactivators pharmacology, Insecticides toxicity, Malathion analogs & derivatives, Oximes pharmacology
- Abstract
Malathion is an organophosphate (OP) pesticide whose toxicity depends on its bioactivation to malaoxon. Human malathion poisoning has been treated with oximes (mainly pralidoxime) in an attempt to reactivate OP-inhibited acetylcholinesterase (AChE). However, pralidoxime has shown unsatisfactory therapeutic effects in malathion poisoning and its routine use has been questioned. In this study, we evaluated the in vitro potency of standards and newly developed oximes in reactivating malaoxon-inhibited AChE derived from mouse brain supernatants. Malaoxon displayed a concentration-dependent inhibitory effect on mouse brain AChE (IC(50) = 2.36 microM), and pralidoxime caused a modest reactivating effect (30% of reactivation at 600 microM). Obidoxime and trimedoxime, as well as K047 and K075, displayed higher reactivating effects (from 55% to 70% of reactivation at 600 muM) when compared with pralidoxime. The results show that obidoxime, trimedoxime, K074 and K075 present higher reactivating effects on malaoxon-inhibited AChE under in vitro conditions when compared with pralidoxime. Taking into account the unsatisfactory effects of pralidoxime as antidotal treatment in malathion poisonings, the present results suggest that obidoxime, trimedoxime, K074 and K075 might be interesting therapeutic strategies to reactivate malaoxon-inhibited AChE in malathion poisonings.
- Published
- 2010
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91. Oxidative stress-mediated inhibition of brain creatine kinase activity by methylmercury.
- Author
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Glaser V, Leipnitz G, Straliotto MR, Oliveira J, dos Santos VV, Wannmacher CM, de Bem AF, Rocha JB, Farina M, and Latini A
- Subjects
- Analysis of Variance, Animals, Astrocytoma pathology, Brain enzymology, Cell Line, Tumor, Dose-Response Relationship, Drug, Enzyme Activation drug effects, Male, Mice, Protein Carbonylation drug effects, Reactive Oxygen Species metabolism, Tetrazolium Salts, Thiazoles, Time Factors, Brain drug effects, Creatine Kinase metabolism, Gene Expression Regulation, Enzymologic drug effects, Methylmercury Compounds pharmacology, Oxidative Stress drug effects
- Abstract
Methylmercury (MeHg), a potent neurotoxicant, easily passes through the blood-brain barrier and accumulates in brain causing severe irreversible damage. However, the underlying neurotoxic mechanisms elicited by MeHg are still not completed defined. In this study, we aimed to investigate the in vitro toxic effects elicited by crescent concentrations (0-1500 microM) of MeHg on creatine kinase (CK) activity, thiol content (NPSH) and protein carbonyl content (PCC) in mouse brain preparations. In addition, CK activity, MTT reduction and DCFH-DA oxidation (reactive oxygen species (ROS) formation) were also measured in C6 glioma cell linage. CK activity was severely reduced by MeHg treatment in mouse brain preparations. This inhibitory effect was positively correlated to the MeHg-induced reduction of NPSH levels and increment in PCC. Moreover, the positive correlation between brain CK activity and NPSH levels was observed at either 15 or 60 min of MeHg pre-incubation. In addition, MeHg-treated C6 cells showed also a significant inhibition of CK activity at MeHg concentrations, as low as, 50 microM in parallel to reduced mitochondrial function and increased ROS production. Taking together, these data demonstrate that MeHg severely affects CK activity, an essential enzyme for brain energy buffering to maintain cellular energy homeostasis. This effect appears to be mediated by oxidation of thiol groups that might cause subsequent oxidative stress., (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Published
- 2010
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92. Effects of environmental and artificial UV-B radiation on freshwater prawn Macrobrachium olfersi embryos.
- Author
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Nazari EM, Ammar D, de Bem AF, Latini A, Müller YM, and Allodi S
- Subjects
- Animals, Embryo, Nonmammalian anatomy & histology, Embryo, Nonmammalian cytology, Embryo, Nonmammalian radiation effects, Environment, Female, Fresh Water, Male, Mitosis, Oxidative Stress radiation effects, Palaemonidae anatomy & histology, Palaemonidae cytology, Sulfhydryl Compounds metabolism, Thiobarbituric Acid Reactive Substances metabolism, Palaemonidae radiation effects, Ultraviolet Rays
- Abstract
The recent decrease of the stratospheric ozone has resulted in an increase of ultraviolet-B (UV-B) radiation reaching the Earth's surface. In freshwater ecosystems with transparent water, UV-B rays easily penetrate and potentially cause harmful effects to organisms. In this study, embryos of the prawn Macrobrachium olfersi were used to evaluate the impact of UV-B rays in freshwater environments. We observed three groups of embryos: the first was to assess whether UV-B radiation produced morphological defects and/or biochemical impairments in the laboratory. The second was to check whether embryos with the same impairments as those observed in the laboratory were found in their environment, under natural solar radiation. The third group was the non-irradiated control. The embryos irradiated with 310 mW cm(-2) UV-B for 30 min showed morphological alterations similar to those observed in embryos from the environmental control group. The most important effects of the UV-B radiation observed in M. olfersi embryos were morphological (1.2% of the total number of embryos from the environment and 2.8% of the total number of irradiated embryos), pigmentation changes in the eyes (78.0% of the total number of embryos from the environment and 98.9% of the total number of irradiated embryos), and disruption of the chromatophores (46.9% of the total number of embryos from the environment and 95.5% of the total number of irradiated embryos). We also observed an increase in egg volume, which was accompanied by a significant increase in water content in UV-B irradiated groups when compared with aquaria control embryos. In addition, a significant decrease in the mitotic index in eggs exposed to UV-B radiation was detected (0.17 for the embryos from the aquaria control, 0.10 for the embryos of the environmental control, and 0.04 for the irradiated groups). The low levels of NPSH and high levels of TBARS indicated that UV-B rays directly compromised the antioxidant function of the embryonic cells, leading to oxidative stress. Our combined morphological and biochemical analyses revealed important effects induced by UV-B on M. olfersi embryos, and the results suggest that the recent changes in global conditions may have injurious effects, at least on the embryos of freshwater prawns., (Copyright (c) 2010 Elsevier B.V. All rights reserved.)
- Published
- 2010
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93. Diphenyl diselenide decreases serum levels of total cholesterol and tissue oxidative stress in cholesterol-fed rabbits.
- Author
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de Bem AF, Portella Rde L, Colpo E, Duarte MM, Frediane A, Taube PS, Nogueira CW, Farina M, da Silva EL, and Teixeira Rocha JB
- Subjects
- Animal Feed, Animals, Ascorbic Acid analysis, Brain drug effects, Brain enzymology, Brain Chemistry drug effects, Hypercholesterolemia chemically induced, Liver chemistry, Liver drug effects, Liver enzymology, Male, Rabbits, Random Allocation, Reactive Oxygen Species blood, Thiobarbituric Acid Reactive Substances analysis, Triglycerides blood, Antioxidants pharmacology, Benzene Derivatives pharmacology, Cholesterol blood, Cholesterol, Dietary administration & dosage, Organoselenium Compounds pharmacology, Oxidative Stress drug effects, Porphobilinogen Synthase blood, Reactive Oxygen Species metabolism
- Abstract
Hypercholesterolaemia and oxidative stress are well-known risk factors in coronary artery diseases. Diphenyl diselenide is a synthetic organoselenium compound that has been shown to have in vitro and in vivo antioxidant properties. In this study, we investigated whether diphenyl diselenide could reduce the hypercholesterolaemia and diminish the tissue oxidative stress in cholesterol-fed rabbits. Twenty-four New Zealand white male rabbits were randomly divided into four groups. Each group was fed a different diet as follows: Control group--regular chow; Cholesterol group--1% cholesterol-enriched diet; diphenyl diselenide group--regular diet supplemented with 10 ppm diphenyl diselenide; and Chol/diphenyl diselenide group--the same cholesterol-rich supplemented with 10 ppm diphenyl diselenide. After 45 days of treatment, the rabbits were killed and the blood, liver, and brain were used for laboratory analysis. The results showed that the serum levels of total cholesterol were markedly increased in cholesterol-fed rabbits and the consumption of diphenyl diselenide decreased these levels approximately twofold in Chol/diphenyl diselenide rabbits (P < 0.05). The intake of diphenyl diselenide by hypercholesterolaemic rabbits diminished the serum and hepatic thiobarbituric acid reactive substances levels as well as the production of reactive oxygen species in the blood and brain (P < 0.05) when compared to the cholesterol group. In addition, diphenyl diselenide supplementation increased hepatic and cerebral delta-aminolevulinic dehydratase activity and hepatic non-protein thiol groups levels despite hypercholesterolaemia (P < 0.05). In summary, the results showed that diphenyl diselenide reduced the hypercholesterolaemia and the oxidative stress in cholesterol-fed rabbits.
- Published
- 2009
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94. Synergistic neurotoxicity induced by methylmercury and quercetin in mice.
- Author
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Martins Rde P, Braga Hde C, da Silva AP, Dalmarco JB, de Bem AF, dos Santos AR, Dafre AL, Pizzolatti MG, Latini A, Aschner M, and Farina M
- Subjects
- Animals, Dose-Response Relationship, Drug, Female, Mice, Oxidative Stress, Methylmercury Compounds toxicity, Nervous System drug effects, Quercetin toxicity
- Abstract
Methylmercury (MeHg) is a highly neurotoxic pollutant, whose mechanisms of toxicity are related to its pro-oxidative properties. A previous report showed under in vivo conditions the neuroprotective effects of plants of the genus Polygala against MeHg-induced neurotoxicity. Moreover, the flavonoid quercetin, isolated from Polygala sabulosa, displayed beneficial effects against MeHg-induced oxidative damage under in vitro conditions. In this study, we sought for potential beneficial effects of quercetin against the neurotoxicity induced by MeHg in Swiss female mice. Animals were divided into six experimental groups: control, quercetin low dose (5 mg/kg), quercetin high dose (50 mg/kg), MeHg (40 mg/L, in tap water), MeHg+quercetin low dose, and MeHg+quercetin high dose. After the treatment (21 days), a significant motor deficit was observed in MeHg+quercetin groups. Biochemical parameters related to oxidative stress showed that the simultaneous treatment with quercetin and MeHg caused a higher cerebellar oxidative damage when compared to the individual exposures. MeHg plus quercetin elicited a higher cerebellar lipid peroxidation than MeHg or quercetin alone. The present results indicate that under in vivo conditions quercetin and MeHg cause additive pro-oxidative effects toward the mice cerebellum and that such phenomenon is associated with the observed motor deficit.
- Published
- 2009
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95. 17β-estradiol decreases methylmercury-induced neurotoxicity in male mice.
- Author
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Malagutti KS, da Silva AP, Braga HC, Mitozo PA, Soares Dos Santos AR, Dafre AL, de Bem AF, and Farina M
- Abstract
There is increasing evidence that health effects of toxic metals, including methylmercury (MeHg), differ in prevalence or are manifested differently in men and women. The present study was aimed at investigating the potential differential susceptibility of male and female Swiss mice against MeHg-induced neurotoxicity, which was evaluated by biochemical (cerebellar oxidative stress-related parameters) and behavioral (locomotor activity and motor performance) variables. We also aimed to evaluate the potential protective effects of 17β-estradiol against such toxicity in MeHg-exposed male animals. MeHg exposure (40mg/L, diluted in tap water, during 2 weeks) decreased locomotor activity and motor performance in both male and female animals, but such phenomena were higher in males. 17β-estradiol co-treatment (10μg/animal, in alternate days) prevented MeHg-induced locomotor deficits in males. MeHg exposure caused a significant increase (60%) in cerebellar lipid peroxidation in male mice, but did not in females. In close agreement, MeHg exposure decreased (43%) cerebellar glutathione peroxidase activity in males, but did not in females. These events were prevented by 17β-estradiol administration. Cerebellar GR activity was increased (25%) in MeHg-exposed males and such event was partially prevented by 17β-estradiol administration. These results indicate that the low susceptibility of female mice to the neurotoxicity elicited by MeHg is linked to neuroprotective effects of sex steroids, which appear to modulate the activities of glutathione-related enzymes. Our experimental observation corroborates previous epidemiological studies showing the greater developmental effects in male than in female humans exposed to MeHg., (Copyright © 2008 Elsevier B.V. All rights reserved.)
- Published
- 2009
- Full Text
- View/download PDF
96. Oximes as inhibitors of low density lipoprotein oxidation.
- Author
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de Lima Portella R, Barcelos RP, de Bem AF, Carratu VS, Bresolin L, da Rocha JB, and Soares FA
- Subjects
- Copper Sulfate pharmacology, Dose-Response Relationship, Drug, Humans, In Vitro Techniques, Lipoproteins, LDL chemistry, Molecular Structure, Organophosphate Poisoning, Oxidation-Reduction, Oximes chemistry, Poisoning blood, Poisoning drug therapy, Thiobarbituric Acid Reactive Substances metabolism, Lipoproteins, LDL blood, Oximes pharmacology
- Abstract
Aims: Several lines of evidence support the hypothesis that the oxidation of low density lipoprotein (LDL) may play a crucial role in the initiation and progression of atherosclerosis. Various studies have shown a positive effect of antioxidant compounds on oxidative modification of LDL and atherogenesis. In view of this, we have investigated the possible antioxidant activity of two new oximes against Cu2+- induced LDL and serum oxidation. Oximes are used in organophosphate (OP) poisoning acting by restoring the cholinesterase function. However, their antioxidant capacities are not well understood and poorly studied., Main Methods: We measured, in a Cu2+-induced oxidation, the conjugated dienes formation in serum and LDL and the loss of tryptophan fluorescence as well as the TBARS formation in the LDL., Key Findings: Our results showed that both oximes act as antioxidant and they are able to prevent LDL oxidation in a concentration-dependent manner. When human LDL or serum was oxidized by Cu2+, our oximes showed a significant increase in the lag phase of conjugated dienes and a significant decrease in the thiobarbituric acid reactive substances production. Moreover, oximes protected tryptophan residues of ApoB-100 in the early stage of LDL oxidation and during the subsequent propagation phase., Significance: These results indicated for the first time that oximes have a potential antioxidant activity and they could act in the prevention of LDL and serum oxidation. Thus, we speculated that our oximes could act as antiatherogenic compounds besides their well described role as antidote for organophosphate poisoning.
- Published
- 2008
- Full Text
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97. Diphenyl diselenide, a simple glutathione peroxidase mimetic, inhibits human LDL oxidation in vitro.
- Author
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de Bem AF, Farina M, Portella Rde L, Nogueira CW, Dinis TC, Laranjinha JA, Almeida LM, and Rocha JB
- Subjects
- Animals, Aorta pathology, Cell Culture Techniques, Humans, Lipoproteins, LDL metabolism, Oxidative Stress drug effects, Rats, Rats, Wistar, Thiobarbituric Acid Reactive Substances, Aorta drug effects, Benzene Derivatives pharmacology, Endothelial Cells drug effects, Glutathione Peroxidase metabolism, Lipid Peroxidation drug effects, Lipoproteins, LDL drug effects, Organoselenium Compounds pharmacology
- Abstract
Oxidative modification of low-density lipoprotein (LDL) represents an important factor in atherogenesis. In the present study, we have investigated the antioxidant capability of diphenyl diselenide (PhSe)(2), a simple organoseleno compound, against copper (Cu2+) and peroxyl radical-induced human LDL oxidation in vitro. In initial studies using human serum, (PhSe)(2) caused a dose-dependent inhibition of Cu(2+)-induced lipid peroxidation, which was correlated to thiol consumption. (PhSe)(2) increased lipid peroxidation lag phase and decreased lipid peroxidation rate in isolated human LDL, evaluated by measuring both conjugated diene (CD) and thiobarbituric acid reactive substances (TBARS) levels. Consistent with these observations, (PhSe)(2) showed a marked inhibitory effect on 2,2-azobis(2-amidinopropane dihydrochloride) (AAPH)-induced oxidation of LDL or parinaric acid (PnA) incorporated into LDL. (PhSe)(2) also displayed a dose-dependent protective effect against Cu(2+)-induced lipid peroxidation in rat aortic slices. Interestingly, besides the antioxidant effects of (PhSe)(2) toward the lipid moieties of LDL, which was related to its thiol-peroxidase activity, protein moieties from human isolated LDL were also protected against Cu(2+)-induced oxidation. The results presented herein are the first to show that (i) (PhSe)(2) inhibits lipid peroxidation in human isolated LDL in vitro, (ii) this phenomenon is related to its thiol-peroxidase activity, and (iii) this chalcogen also prevents the oxidation of protein moieties of human LDL. Taken together, such data render (PhSe)(2) a promising molecule for pharmacological studies with respect to the atherogenic process.
- Published
- 2008
- Full Text
- View/download PDF
98. Plasmatic vitamin C in nontreated hepatitis C patients is negatively associated with aspartate aminotransferase.
- Author
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Souza dos Santos RM, de Bem AF, Colpo E, Bertoncello I, Nogueira CW, and Rocha JB
- Subjects
- Adult, Analysis of Variance, Catalase blood, Female, Glutathione Peroxidase blood, Humans, Male, Thiobarbituric Acid Reactive Substances analysis, Ascorbic Acid blood, Aspartate Aminotransferases blood, Biomarkers blood, Hepatitis C blood, Oxidative Stress
- Abstract
Objectives: To evaluate the possible relationship between aminotransferases levels and markers of oxidative stress in chronic hepatitis C patients., Design and Methods: Patients without treatment for hepatitis were divided in to group I (15-39 U/L); group II (41-76 U/L) and group III (81-311 U/L) of activity alanine aminotransferase (ALT). Blood markers of oxidative stress [catalase (CAT), glutathione peroxidase (GPx), thiobarbituric acid-reactive species (TBARS), nonprotein and protein thiol (NP-SH and P-SH) groups and vitamin C] were determined., Results: P-SH and NP-SH levels, TBARS, GPx and CAT were not different between groups. Vitamin C was significantly decreased in groups II (P=0.03) and III (P=0.001) when compared with group I and correlated negatively with aspartate aminotransferase (AST; r=-0.29, P=0.042)., Conclusion: Vitamin C levels were negatively associated with AST, suggesting that vitamin C could be an additional indicator of hepatitis C severity.
- Published
- 2008
- Full Text
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99. A single high dose of ascorbic acid and iron is not correlated with oxidative stress in healthy volunteers.
- Author
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Colpo E, de Bem AF, Pieniz S, Schettert SD, dos Santos RM, Farias IL, Bertoncello I, Moreira CM, Barbosa NV, Moretto MB, and Rocha JB
- Subjects
- Adult, Antioxidants metabolism, Ascorbic Acid adverse effects, Ascorbic Acid blood, Ascorbic Acid pharmacokinetics, Catalase blood, Cross-Over Studies, Drug Interactions, Ferritins blood, Humans, Iron blood, Iron, Dietary adverse effects, Iron, Dietary blood, Iron, Dietary pharmacokinetics, Male, Oxidation-Reduction, Porphobilinogen Synthase blood, Thiobarbituric Acid Reactive Substances analysis, Young Adult, Ascorbic Acid pharmacology, Iron, Dietary pharmacology, Oxidative Stress drug effects
- Abstract
Fe (II) is a potential prooxidant in vivo and can induce cellular oxidative stress. Ascorbic acid (AA) is a powerful physiological antioxidant and, in the presence of free Fe (II), can exhibit prooxidant effects in vitro. However, in vivo prooxidant effects of Fe (II) and AA have not yet been indisputably demonstrated. Here we evaluate the potential toxic effect of supplementation of Fe (II) associated with AA. Nine healthy, nonsmoking male volunteers (20-31 years old) participated in the crossover study design. The volunteers were supplemented with either a dose of 2 g of AA, 150 mg of iron carbonyl or 2 g of AA plus 150 mg of iron carbonyl with a washout period of 15 days between each treatment. AA, iron, ferritin, thiobarbituric acid-reactive substances, catalase, delta-aminolevulinic dehydratase and SH thiol groups were measured in the blood of the volunteers. Plasma AA levels were increased at 2, 5 and 24 h after AA or AA plus iron ingestion. Plasma Fe levels were increased at 2 and 5 h in the AA plus iron group. Erythrocyte malondialdehyde levels decreased at 5 and 24 h after AA and 5 h after AA plus iron ingestion. Catalase activity from erythrocytes was increased 5 h after supplementation with AA plus iron. There was no significant difference between groups in the other biochemical parameters evaluated. Thus, the present study does not support the hypothesis that the combination of high plasma concentrations of AA and iron, or iron alone, could cause in vivo oxidative damage after a single supplementation dose., (2008 S. Karger AG, Basel)
- Published
- 2008
- Full Text
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100. Low toxicity of diphenyl diselenide in rabbits: a long-term study.
- Author
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de Bem AF, de Lima Portella R, Farina M, Perottoni J, Paixão MW, Nogueira CW, and Teixeira Rocha JB
- Subjects
- Animals, Antioxidants pharmacokinetics, Ascorbic Acid metabolism, Benzene Derivatives pharmacokinetics, Catalase metabolism, Dose-Response Relationship, Drug, Glutathione Peroxidase metabolism, Lipid Peroxidation physiology, Male, Organoselenium Compounds pharmacokinetics, Porphobilinogen Synthase metabolism, Rabbits, Thiobarbituric Acid Reactive Substances metabolism, Time Factors, Antioxidants adverse effects, Benzene Derivatives adverse effects, Brain metabolism, Kidney metabolism, Liver metabolism, Organoselenium Compounds adverse effects
- Abstract
Selenium compounds, like diphenyl diselenide (Ph(2)Se(2)), possess glutathione peroxidase (GSHPx)-like activities and other antioxidant properties. The aim of this study was to evaluate the effects of a long-term oral supplementation with Ph(2)Se(2) on various toxicological parameters in rabbits. Adult New Zealand male rabbits were divided into four groups: Group I served as control; Groups II, III and IV received 0.3, 3.0 and 30 p.p.m. of Ph(2)Se(2) pulverized in the chow for 8 months. A number of toxicological parameters were examined in liver, kidney, cerebral cortex and hippocampus, such as delta-aminolaevulinic acid dehydratase (delta-ALA-D), catalase (CAT), GSHPx activities, non-protein thiol (-SH), lipid peroxidation and ascorbic acid levels. The results indicated that supplementation 30 p.p.m. Ph(2)Se(2 )significantly increased delta-ALA-D activity in liver and in cerebral cortex. Non-protein -SH levels were significantly increased in liver but not in kidney, cerebral cortex and hippocampus of rabbits. Ascorbic acid content was significantly lower in the liver and cerebral cortex after supplementation with 30 p.p.m. Ph(2)Se(2). Conversely, no alterations in GSHPx and CAT activities, nor in thiobarbituric acid reactive substances levels were observed in rabbit tissues. These results indicate that oral supplementation with Ph(2)Se(2) is relatively secure in rabbits after 8 months of exposure. The findings encourage further experiments on the potential therapeutic effects of such compound.
- Published
- 2007
- Full Text
- View/download PDF
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