Search

The Fused Filament Fabrication (FFF) method is an additive technology that is used for the creation of prototypes within Rapid Prototyping (RP) as well as for the creation of final components in piece or small-series production. The possibility of using FFF technology in the creation of final products requires knowledge of the properties of the material and, at the same time, how these properties change due to degradation effects. In this study, the mechanical properties of the selected materials (PLA, PETG, ABS, and ASA) were tested in their non-degenerate state and after exposure of the samples to the selected degradation factors. For the analysis, which was carried out by the tensile test and the Shore D hardness test, samples of normalized shape were prepared. The effects of UV radiation, high temperature environments, high humidity environments, temperature cycles, and exposure to weather conditions were monitored. The parameters obtained from the tests (tensile strength and Shore D hardness) were statistically evaluated, and the influence of degradation factors on the properties of individual materials was assessed. The results showed that even between individual manufacturers of the same filament there are differences, both in the mechanical properties and in the behavior of the material after exposure to degradation effects., Metoda Fused Filament Fabrication (FFF) je aditivní technologie, která se používá pro tvorbu prototypů v rámci Rapid Prototyping (RP) i pro tvorbu finálních komponent v kusové nebo malosériové výrobě. Možnost využití technologie FFF při tvorbě finálních výrobků vyžaduje znalost vlastností materiálu a současně i toho, jak se tyto vlastnosti mění v důsledku degradačních účinků. V této studii byly testovány mechanické vlastnosti vybraných materiálů (PLA, PETG, ABS a ASA) v nedegenerovaném stavu a po vystavení vzorků vybraným degradačním faktorům. Pro analýzu, která byla provedena tahovou zkouškou a zkouškou tvrdosti podle Shorea D, byly připraveny vzorky normalizovaného tvaru. Byl sledován vliv UV záření, prostředí s vysokou teplotou, prostředí s vysokou vlhkostí, teplotních cyklů a vystavení povětrnostním podmínkám. Parametry získané ze zkoušek (pevnost v tahu a tvrdost Shore D) byly statisticky vyhodnoceny a byl posouzen vliv degradačních faktorů na vlastnosti jednotlivých materiálů. Výsledky ukázaly, že i mezi jednotlivými výrobci stejného vlákna existují rozdíly, a to jak v mechanických vlastnostech, tak v chování materiálu po vystavení degradačním vlivům.

Drilling into the solid material is one of the basic technological operations, which creates a cylindrical hole in an appropriate time with required quality. Drilling operation demands a favourable removal of chips from the cutting area because a creation of an undesirable shape of chips can impart a lower quality of the drilled hole corresponding with the generation of excess heat due to the intense contact of the chip with drill. The solution for a proper machining is a suitable modification of the drill geometry i.e., point and clearance angles as presented in current study. The tested drills are made of M35 high-speed steel characterized by a very thin core at the point of the drill. An interesting feature of the drills is the use of cutting speed higher than 30 m min(-1), with the feed of 0.2 mm per revolution. The surface roughness (Ra and Rz lower than 1 mu m and 6 mu m respectively), cylindricity (0.045 mm), roundness (0.025 mm), perpendicularity of the hole axis (0.025 mm), diameters and position of the individual holes were achieved for a drill with point angle 138.32 degrees and clearance angle 6.92 respectively. The increase of the drill point angle by 6 degrees resulted in the decrease in the feed force of more than 150 N. In addition, an increase of the clearance angle by 1 degrees resulted with a decrease in the feed force of 70 N. The results of the experiment showed that with the correct geometry of the tool the effective machining without using internal cooling can be realised.

Incineration is currently the standard way of disposing of municipal waste. It uses components protected by high-temperature-resistant layers of materials, such as Inconel alloys. Therefore, the objective of the current paper is to study the mechanical properties and structure of a bimetallic Inconel 625-16Mo3 steel tube. The Inconel 625 layer was 3.5 mm thick and was applied to the surface of the tube with a wall thickness of 7 mm via the cold metal transfer method. The bimetallic tube was bent using a supercritical bend (d <= 0.7D). This paper is focused on the investigation of the material changes in the Inconel 625 layer areas influenced by the maximum tensile and compressive stresses after the bend. The change in layer thickness after the bend was evaluated and compared to the non-deformed tube. In addition, the local mechanical properties (nanohardness, Young modulus) across the indicated interfacial areas using quasistatic nanoindentation were investigated. Subsequently, a thorough microstructure observation was carried out in areas with maximum tensile and compressive stresses to determine changes in the morphology and size of dendrites related to the effect of tensile or compressive stresses induced by bending. It was found that the grain featured a stretched secondary dendrite axis in the area of tensile stress, but compressive stress imparted a prolongation of the primary dendrite axis.

This article deals with the ballistic impact of the 7.62 mm FMJ M80 rifle projectile into the laminated Twaron/UHMWPE composite armor. The armor composition consisted of composite panels made from Twaron CT 747 para-aramid fabric and ultra-high-molecular-weight Endumax Shield XF33 polyethylene. To analyze the ballistic impact and to verify the resistance of the designed armor according to the NATO AEP 4569 STANAG standard, protection level 1, 7.62 x 51 mm FMJ NATO M80 rifle cartridges with lead projectiles were used in the ballistic experiment. After the projectile impact, the damage failure mechanisms of the composite panels were documented. As part of the evaluation of the experiments, the initial microstructure of the composite panels was documented, and subsequently, the damaged areas of the composite armor after the ballistic experiment were also documented. Optical and scanning electron microscopy were used to document the structures. The important parameter of composite armor is its mechanical properties. The surface hardness of the composite panels was measured by the Shore D method using the hardness tester DIGI-Test II. The results obtained from the ballistic experiment demonstrate that the designed Twaron/Endumax armor was not penetrated. This armor has sustained multiple impacts for all three 7.62 mm FMJ M80 projectiles and is suitable for the construction of armor protection.

This article aims to investigate the accuracy and dimensional stability of bonded metal and CFRP (Carbon Fibre Reinforced Plastic) adherends. The motivation behind this study was to assess the suitability of CFRP for optical devices through the evaluation of precision bonding technology. A binocular was selected as a reference optical device. A technological sample was designed, with required total runout of key dimensions 0.05 mm. The sample underwent testing according to ISO 9022-1. The total runout was evaluated after production and environmental tests. Eight out of 15 samples were turned after gluing due to insufficient accuracy. None of the turned samples exceeded the total runout deviation of 0.01 mm, and the average value of the maximal deviation was 0.0041 mm. The noncalibrated samples performed significantly worse with the average value of the maximal deviation of 0.0164 mm. The measurements during the climatic tests showed that the largest deviation (on average 77.6% of the maximum achieved deviation) occurs at the first temperature loading. Subsequent temperature cycles caused lower deviations. The results highlight the significance of addressing deformations resulting from adhesive volume shrinkage-induced stress as a crucial factor in precision bonding technology.

This paper deals with the study of high-strength M300 maraging steel produced using the selective laser melting method. Heat treatment consists of solution annealing and subsequent aging; the influence of the selected aging temperatures on the final mechanical properties-microhardness and compressive yield strength-and the structure of the maraging steel are described in detail. The microstructure of the samples is examined using optical and electron microscopy. The compressive test results show that the compressive yield strength increased after heat treatment up to a treatment temperature of 480 degrees C and then gradually decreased. The sample aged at 480 degrees C also exhibited the highest observed microhardness of 562 HV. The structure of this sample changed from the original melt pools to a relatively fine-grained structure with a high fraction of high-angle grain boundaries (72%).

The study of endomembrane trafficking is crucial for understanding how cells and whole organisms function. Moreover, there is a special interest in investigating endomembrane trafficking in plants, given its role in transport and accumulation of seed storage proteins and in secretion of cell wall material, arguably the two most essential commodities obtained from crops. The mechanisms of anterograde transport in the biosynthetic and endocytic pathways of plants have been thoroughly discussed in recent reviews, but, comparatively, retrograde trafficking pathways have received less attention. Retrograde trafficking is essential to recover membranes, retrieve proteins that have escaped from their intended localization, maintain homeostasis in maturing compartments, and recycle trafficking machinery for its reuse in anterograde transport reactions. Here, we review the current understanding on retrograde trafficking pathways in the endomembrane system of plants, discussing their integration with anterograde transport routes, describing conserved and plant-specific retrieval mechanisms at play, highlighting contentious issues and identifying open questions for future research.

Within the herein presented research, we studied the applicability of flax fabrics for composite parts in personal watercrafts in order to enhance damping of vibrations from the engine and noise reduction (which is relatively high for contemporary carbon constructions). Since the composite parts are intended to be exposed to humid environments requiring high levels of mechanical properties, a carbon–flax composite was selected. Samples of carbon, fiberglass, flax, and hybrid carbon–flax twill and biax fabrics were subjected to tensile and three-point bending tests. The mechanical properties were also tested after exposure of the samples to a humid environment. Damping was assessed by vibration and noise measurements directly on the complete float for samples as well as real parts. The hybrid carbon–flax material exhibited lower values of tensile strength than the carbon material (760 MPa compared to 463 MPa), but, at the same time, significantly higher than the other tested materials, or flax itself (115 MPa for a twill fabric). A similar trend in the results was observed for the three-point bending tests. Vibration tests and noise measurements showed reductions in vibration amplitude and frequency when using the carbon–flax hybrid material; the frequency response function for the watercraft part assembled from the hybrid material was 50% lower than for that made of carbon. Testing of samples located in a humid environment showed the necessity of surface treatment to prevent moisture absorption (mechanical properties were reduced at minimum by 28%). The tests confirmed that the hybrid material is satisfactory in terms of strength and its contribution to noise and vibration damping.

The influence of plasma nitriding and gas nitriding processes on the change of surface roughness and dimensional accuracy of 42CrMo4 steel was investigated in this paper. Both processes almost always led to changes in the surface texture. After plasma nitriding, clusters of nitride ions were formed on the surface of steel, while gas nitriding very often led to the new creation of a formation of a “plate-like” surface texture. In both cases of these processes, a compound layer in specific thickness was formed, although the parameters of the processes were chosen with the aim of suppressing it. After the optimizing of nitriding parameters during nitriding processes, it was found that there were no changes in the surface roughness evaluated using the Ra parameter. However, it turned out that when using a multi-parameter evaluation of roughness (the parameters Rz, Rsk and Rku were used), there were presented some changes in roughness due to nitriding processes, which affect the functional behavior of the components. Roughness changes were also detected by evaluating surface roughness profiles, where nitriding led to changes in peak heights and valley depths. Nitriding processes further led to changes in dimensions in the form of an increase of 0.032 mm on average. However, the magnitude of the change has some context on chemical composition of material. A larger increase in dimensions was found with gas nitriding. The change in the degree of IT accuracy is closely related to the change in dimension. For both processes, there was a change of one degree of IT accuracy compared to the ground part (from IT8 to IT9). On the basis of the achieved dimensional accuracy results, a coefficient of change in the degree of accuracy IT was created, which can be used to predict changes in the dimensional accuracy of ground surfaces after nitriding processes in degrees of accuracy IT3–IT10. In this study, a tool for predicting changes in degrees of accuracy of ground parts after nitrid

Vacuolar proteins play essential roles in plant physiology and development, but the factors and the machinery regulating their vesicle trafficking through the endomembrane compartments remain largely unknown. We and others have recently identified an evolutionarily conserved plant endosomal sorting complex required for transport (ESCRT)-associated protein apoptosis-linked gene-2 interacting protein X (ALIX), which plays canonical functions in the biogenesis of the multivesicular body/prevacuolar compartment (MVB/PVC) and in the sorting of ubiquitinated membrane proteins. In this study, we elucidate the roles and underlying mechanism of ALIX in regulating vacuolar transport of soluble proteins, beyond its conventional ESCRT function in eukaryotic cells. We show that ALIX colocalizes and physically interacts with the retromer core subunits Vps26 and Vps29 in planta. Moreover, double-mutant analysis reveals the genetic interaction of ALIX with Vps26 and Vps29 for regulating trafficking of soluble vacuolar proteins. Interestingly, depletion of ALIX perturbs membrane recruitment of Vps26 and Vps29 and alters the endosomal localization of vacuolar sorting receptors (VSRs). Taken together, ALIX functions as a unique retromer core subcomplex regulator by orchestrating receptor-mediated vacuolar sorting of soluble proteins.

CFRP (Carbon Fiber Reinforced Polymers) are often used when designing parts that need to be stiff, light and thermally stable. These benefits are a big motivation to use CFRP in many applications, one of them could be sports optics. However, optical devices require precise dimensions with tight tolerances for the optical assembly to work correctly. In order to determine if CFRP could be a suitable material of choice for sports optics a simplified body of binoculars was designed. The tubular body was manufactured by prepreg lay-up into a 3D printed mold, followed by curing in an autoclave. After the prototype was manufactured 3D measurements of the tube using 3D scanner GOM ATOS were made. As expected, shrinkage of the mold and the epoxy resin in the matrix of CFRP caused minor deformations. However, if the shape of the cured part remains unchanged during conditions similar to the general use of binoculars, then the initial deformations happening during manufacturing could be accounted for when designing the part. © 2020 Manufacturing Technology. All rights reserved.

The factors and mechanisms involved in vacuolar transport in plants, and in particular those directing vesicles to their target endomembrane compartment, remain largely unknown. To identify components of the vacuolar trafficking machinery, we searched for Arabidopsis modified transport to the vacuole (mtv) mutants that abnormally secrete the synthetic vacuolar cargo VAC2. We report here on the identification of 17 mtv mutations, corresponding to mutant alleles of MTV2/VSR4, MTV3/PTEN2A MTV7/EREL1, MTV8/ARFC1, MTV9/PUF2, MTV10/VPS3, MTV11/VPS15, MTV12/GRV2, MTV14/GFS10, MTV15/BET11, MTV16/VPS51, MTV17/VPS54, and MTV18/VSR1. Eight of the MTV proteins localize at the interface between the trans-Golgi network (TGN) and the multivesicular bodies (MVBs), supporting that the trafficking step between these compartments is essential for segregating vacuolar proteins from those destined for secretion. Importantly, the GARP tethering complex subunits MTV16/VPS51 and MTV17/VPS54 were found at endoplasmic reticulum (ER)- and microtubule-associated compartments (EMACs). Moreover, MTV16/VPS51 interacts with the motor domain of kinesins, suggesting that, in addition to tethering vesicles, the GARP complex may regulate the motors that transport them. Our findings unveil a previously uncharacterized compartment of the plant vacuolar trafficking pathway and support a role for microtubules and kinesins in GARP-dependent transport of soluble vacuolar cargo in plants.