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Two highly active anticancer Pt(II) complexes, [Pt(Jat1)Cl]Cl (Pt1) and [Pt(Jat2)Cl]Cl (Pt2), containing jatrorrhizine derivative ligands (Jat1 and Jat2) are described. Cell intake study showed high accumulation in cell nuclear fraction. Pt1 and Pt2 exhibited high selectivity for HeLa cancer cells (IC50 = 15.01 ± 1.05 nM and 1.00 ± 0.17 nM) comparing with HL-7702 normal cells (IC50 > 150 μM), by targeting p53 and telomerase. Pt2 containing Jat2 ligand was more potent and showed high selectivity for telomerase. It also caused mitochondria and DNA damage, sub-G1 phase arrest, and a high rate of cell apoptosis at the low dose of 1.00 nM. The HeLa tumor inhibition rate (TIR) of Pt2 was 48.8%, which was even higher than cisplatin (35.2%). In addition, Pt2 displayed green luminescent property and potent telomerase inhibition. Our findings demonstrated the promising development of platinum(II) complexes containing jatrorrhizine derivatives as novel Pt-based anti-cancer agents.

A series of novel organoplatinum(II) complexes, [PtII(QC1)(H-QC1)Cl] (Pt1), [PtII(QC2)(H-QC2)Cl] (Pt2), [PtII(QC3)(H-QC3)Cl] (Pt3), [PtII(QC4)(H-QC4)Cl]⋅CH3OH (Pt4), [PtII(QC5)(H-QC5)Cl] (Pt5), [PtII(H-QC6)(DMSO)Cl2] (Pt6), [PtII(H-QC7)(DMSO)Cl2]⋅H2O (Pt7), [PtII(H-QC8)(DMSO)Cl2] (Pt8), [PtII(H-QC9)(DMSO)Cl2]⋅CH3OH (Pt9), [PtII(H-QC10)(DMSO)Cl2] (Pt10) and [PtII(H-QC11)(DMSO)Cl2] (Pt11), bearing quinoline-coumarin derivatives (H-QC1−H-QC11) have been first designed. Complexes Pt1–Pt11 selectively displayed obvious cytotoxicities in comparison to cisplatin for A549/DDP (cisplatin-resistant human lung adenocarcinoma) cells and HeLa cervical carcinoma cells, with IC50 values as low as 100 nM−10.33 μM. In addition, Pt4 and Pt5 display a green-colored luminescent properties, targeted mitochondrial membrane and, thereby induced mainly mitochondria-mediated cell apoptosis was in the following order: Pt4 > Pt5. The different anti-cancer activity of quinoline-coumarin complexes Pt4 (100 nM) and Pt5 (250 nM) were correlate with the presence of 3-(2′-quinolyl)-6-hydroxy-coumarin (H-QC4) ligand. The quinoline-coumarin complex Pt4 (2.0 mg/kg per 2 days) also displayed potent in vivo anti-tumor effect after 21 days-treated. In contrast, the H-QC4 ligand highly enhances the anti-tumor activity and selectivity of organoplatinum(II) complexes in comparison to other previously reported coumarin derivatives metal complexes.

Four platinum(II/IV) complexes, [PtIV(PPy)2Cl2]·CH3OH (Pt1), [PtIV(TFPy)2Cl2] (Pt2), [PtII(MPy)(H-MPy)Cl] (Pt3), and [PtII(DPPy)(H-DPPy)Cl] (Pt4) with 2-phenylpyridine (H-PPy), 2-(4-trifluoromethylphenyl)pyridine (H-TFPy), 3-methyl-2-phenylpyridine (H-MPy) and 2,5-diphenylpyridine (H-DPPy) were first designed and prepared. Cytotoxic properties of Pt1–Pt4 were evaluated in human tumor HeLa (cervical), MCF-7 (breast), Hep-G2 (hepatoma), T-24 (bladder) cells and HL-7702 nontumorigenic cells by means of the MTT assay. The four Pt complexes Pt1–Pt4 were more selective for HeLa (cervical) cells versus normal HL-7702 cells. Remarkably the most active Pt2 compound, having 2-(4-trifluoromethylphenyl)pyridine (H-TFPy) ligand, showed IC50 values down to 1.01 ± 0.34 μM. Importantly, Pt1 and Pt2 induce apoptosis in T-24 (bladder) cells via ROS-mediated mitochondria dysfunction.

Two new quinolone ruthenium(II)-arene complexes, i.e., [Ru(cym)(DClQ)] (Ru1) and [Ru(cym)(ClQ)] (Ru2), with 5,7-dichloro-8-hydroxyquinoline (H-DClQ), 5-chloro-8-hydroxyquinoline (H-ClQ) and [(η6-p-cymene)RuI2]2 (cym), were first designed. The cytotoxic activity of two new quinolone Ru(II)-arene complexes Ru1 and Ru2 has been evaluated in T-24 bladder carcinoma, Hep-G2 hepatoma, BEL-7404 hepatocellular carcinoma, MGC80-3 gastric adenocarcinoma, and non-tumorous HL-7702 liver cells in order to study structure-activity relationships of their anti-tumor activity. Additionally, the cytotoxic activity of quinolone Ru(II)-arene complex Ru1 was much higher potent than Ru2 and cisplatin, with low IC50 values was 0.02 ± 0.0.1 μM against T-24 bladder carcinoma cells. The mechanism studies testified that quinolone Ru(II)-arene complexes Ru1 and Ru2 induced apoptosis in T-24 bladder carcinoma cells by generating ROS metabolites, triggering caspase-9 increase and mitochondrial membrane potential loss. The quinolone Ru(II)-arene complex Ru1 with 5,7-dichloro-8-hydroxyquinoline (H-DClQ) ligand was considerably more active than the 5-chloro-8-hydroxyquinoline Ru2 complex.

Best Management Practices (BMPs) is a good way to control pollution source. Now many BMPs have been collected and stored in database. But they are not available directly by managers due to cognitive differences. In order to recommend suitable BMP to water environment managers for treating specific contaminated river, we model BMP knowledge with ontology. In the paper, we introduce ontology building process, and a wastewater BMP ontology is built and integrated into an environment management decision-making support system. In the end, a BMP recommendation case study is also presented.

Three critical technologies of simulation analysis on thermal-loads of radome in flight are selected to study in this paper. Section 2 analyzes the first critical technology, which is modeling of the radome and the antenna. And the computational fluid dynamics software FLUENT is used to analyse the radome, so as to calculate the temperature distribution of its outer surface. Section 3 analyzes the second critical technology, which is to establish the multi-cavity thermal radiation model of the radome so that to get the temperature distribution of the inner surface. A kind of interpolation method, which is the third critical technology, is used to transfer the temperature of flow field and the space heat radiation to the corresponding thermal-stress analysis model of radome.

The formation of PrOCl is widely regarded as a result of PrCl3 hydrolysis; that is, PrCl3 + H2 O = PrOCl + HCl. However, our study indicates that PrCl3 hydrolysis follows the schematic PrCl3 → Pr(OH)2 Cl → PrOCl. In this study, several analytical techniques, such as X-ray diffraction, field-emission scanning electron microscopy (FESEM), thermogravimetry (TG)–differential thermal analysis (DTA) and TG–DTA–mass spectrometry, were employed to explore the mechanism underlying the thermal decomposition of PrCl3 ·6H2 O at 25 – 1500 °C in air atmosphere. To examine the hydrolysis mechanism of PrCl3 , the hydrolysis product was separated by continuous centrifugation. The phase composition and morphology of the intermediate compound were determined by XRD and FESEM, respectively. Finally, the dehydration schematic and oxidation behavior of PrCl3 were discussed according to the evolved gas composition.

To explore the clinical efficacy of endovascular treatment for lower extremity arteriosclerosis obliterans.A total of 34 patients (43 limbs) with lower extremity arteriosclerosis obliterans undergoing endovascular treatment from July 2010 to July 2012 were analyzed. The cohort had Fontaine stage II b (n = 16), Fontaine stage III (n = 10) and Fontaine stage III (n = 8). Among them, the lesions were of TASC IIA (n = 4), TASC IIB (n = 8), TASC IIC (n = 9) and TASC IID (n = 13). All patients underwent percutaneous transluminal angioplasty (PTA) plus stents after computed tomographic angiography (CTA) or digital subtraction angiography (DSA). And 9 patients underwent femoral endarterectomy associated with endovascular treatment.The success rate of this technique was 100%. One iliac artery ruptured during endovascular surgery. One patient suffered intraoperatively from arterial perforation. One patient was amputated one week later. One patient had in-stent thrombosis at Week 3 post-treatment. One patient was amputated at Week 4 after endovascular treatment. Thirty patients (38 limbs) were followed up for 13.3 months. During the follow-up, 3, 4 and 4 limbs became occluded at Month 6, 12 and 24 post-treatment respectively.Endovascular treatment has an excellent early patency rate. Special issues should be properly handled during endovascular treatment.

Recently, the research of flow structure of hypervelocity athodyd firebox is very popular [1], especially, to realize the three dimension flow structure of cut layer and eddy is more exigent, therefore, many flow visualization techniques have been developed, such as the particle image velocimetry (PIV). The focusing schlieren method is good in three dimension flow visualization for it needn’t put any materials in the flow [2, 3, 4], The focusing schlieren system to show the flow fields of two sections in the research of hypervelocity firebox fuel branch wake structure has been developed, which can be used also to validate the ability to show the three dimension flow. The system can get two images of two different sections flow at the same time in one experiment, if the technique has been breached, it will be used to show more sections is promising.

Large-size of spatial data and limited bandwidth of network make it restricted to transmit spatial data in WebGIS. This paper employs IPv6 (Internet Protocol version 6), the successor of IPv4 running now, to transmit spatial data efficiently. As the core of NGN (Next Generation Network), IPv6 brings us many advantages to resolve performance problems in current IPv4 network applications. Multicast, which is mandatory in IPv6 routers, can make one server serve many clients simultaneously efficiently, thus to improve capacity of network applications. The new type of anycast address in IPv6 will make network client applications possible to find the nearest server. This makes data transmission between client and server fastest. The paper introduces how to apply IPv6 multicast and anycast in WebGIS to transmit data efficiently.

The inherent flaws in current network, especially the inadequate IP address space, make the born of NGN (Next Generation Network). The NGN aims to enable the information sharing and inter-operation of heterogeneous networks. As the core of NGN, IPv6 (Internet Protocol version 6), which is designed as the successor of IPv4 running now, brings us many new features. It provides larger address space, supports mobile devices, and has built-in security policy, and so on. Although it brings us so many advantages and the backbone of NGN also has been constructed in many countries, we have difficulties moving to NGN. One of the main reasons is there are few killer applications for it. So what features can be employed by GIS, and how to use them is the main content of this paper. The IPv6 certainly offers new entry to solve some problems in GIS.

In this paper, the development of a dual-function leak detector is presented. The system consists of a laser interference instrument, a portable helium leakage detector, a specially designed test chamber with a quartz-glass observation window, a pressure gauge. The developed measuring system offers new features for experimental investigation on the integrity of hermetic sealed structure. Both helium bombing mode and diaphragm deflection mode were investigated using the developed detector. Basically, the system can be used for helium leak bombing detection. By employing this system for leakage detection in a micro optical switch, it was shown that a leakage of less than 10 -7 std cc/sec can be measured. The system can also be used to measure the surface deflection of a diaphragm. The measurement was accomplished by using laser interference technique to monitor the pressure variation within the small cavity of MEMS devices after pressurized gas was introduced. A leakage as low as 10 -14 std cc/sec could be detected for a sample with several cubic millimeter cavity of 10 -4 mbar.