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As one of the core transmission components, axle-box bearings have been widely applied in urban rail vehicles and played a crucial role in their safe and stable operation. No matter in the starting and stopping stage or stable operation stage of the vehicle, the skidding phenomenon in axle-box bearings exists and impacts their own dynamic characteristics. Furthermore, as one of the main shape errors of the bearing surface, waviness may cause fluctuations for skidding characteristics of axle-box bearings. This study investigates the skidding dynamic analysis of axle-box bearings, taking into account race surface waviness. Firstly, a dynamics model for double-row cylindrical roller bearings is established, considering the cage flexibility and friction forces between different components. Then, this model is coupled with a three-dimension vehicle–track model, allowing for more precise dynamic simulation of axle-box bearings under actual running conditions. Furthermore, an excitation related to race surface waviness is added to the coupling model through time-varying displacement and contact stiffness. At last, the influence of race surface waviness on skidding characteristics of axle-box bearings is analyzed. The effectiveness of the proposed model is validated through theoretical analysis and field tests. Simulation results demonstrate that race surface waviness can impact the cage and roller slip ratio of axle-box bearings. Overall, this study provides valuable insights into the skidding dynamic analysis of axle-box bearings, highlighting the importance of considering race surface waviness in designing and maintaining axle-box bearings. [ABSTRACT FROM AUTHOR]

The article explores the deep crustal structure of the Greenland–Iceland–Faroe Ridge in the North Atlantic, focusing on the characteristics of the Iceland region. Geophysical techniques were used to study the Bouguer gravity anomaly and Moho depth values, revealing significant differences in density and crustal thickness in the study area. The research highlights the development of deep faults and structural zones in the region, providing valuable insights into the tectonic evolution of the Greenland–Norwegian Sea. [Extracted from the article]

Chromatin remodelers are essential for regulating plant growth, development, and responses to environmental stresses. HIT4 (HEAT-INTOLERANT 4) is a novel stress-induced chromatin remodeling factor that has been less studied in abiotic stress and stress resistance, particularly in cotton. In this study, we conducted a comprehensive analysis of the members of the HIT4 gene family in Gossypium hirsutum using bioinformatics methods, including phylogenetic relationships, gene organization, transcription profiles, phylogenetic connections, selection pressure, and stress response. A total of 18 HIT4 genes were identified in four cotton species, with six HIT4 gene members in upland cotton. Based on the evolutionary relationships shown in the phylogenetic tree, the 18 HIT4 protein sequences were classified into four distinct subgroups. Furthermore, we conducted chromosome mapping to determine the genomic locations of these genes and visually represented the structural characteristics of HIT4 in G. hirsutum. In addition, we predicted the regulatory elements in HIT4 in G. hirsutum and conducted an analysis of repetitive sequences and gene collinearity among HIT4 in four cotton species. Moreover, we calculated the Ka/Ks ratio for homologous genes to assess the selection pressure acting on HIT4. Using RNA-seq, we explored the expression patterns of HIT4 genes in G. hirsutum and Gossypium barbadense. Through weighted gene co-expression network analysis (WGCNA), we found that GHHIT4_4 belonged to the MEblue module, which was mainly enriched in pathways such as DNA replication, phagosome, pentose and glucuronate interconversions, steroid biosynthesis, and starch and sucrose metabolism. This module may regulate the mechanism of upland cotton resistance to Verticillium wilt through DNA replication, phagosome, and various metabolic pathways. In addition, we performed heterologous overexpression of GH_D11G0591 (GHHIT4_4) in tobacco, and the results showed a significant reduction in disease index compared to the wild type, with higher expression levels of disease resistance genes in the transgenic tobacco. After conducting a VIGS (virus-induced gene silencing) experiment in cotton, the results indicated that silencing GHHIT4_4 had a significant impact, the resistance to Verticillium wilt weakened, and the internode length of the plants significantly decreased by 30.7% while the number of true leaves increased by 41.5%. qRT-PCR analysis indicated that GHHIT4_4 mainly enhanced cotton resistance to Verticillium wilt by indirectly regulating the PAL, 4CL, and CHI genes. The subcellular localization results revealed that GHHIT4_4 was predominantly distributed in the mitochondria and nucleus. This study offers preliminary evidence for the involvement of the GHHIT4_4 in cotton resistance to Verticillium wilt and lays the foundation for further research on the disease resistance mechanism of this gene in cotton. [ABSTRACT FROM AUTHOR]