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51. Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection

Author

Goldmann, Torsten, Burgemeister, Renate, Sauer, Ulrich, Loeschke, Siegfried, Lang, Dagmar Silvia, Branscheid, Detlev, Zabel, Peter, and Vollmer, Ekkehard

Subjects
Short Report, lcsh:Pathology, lcsh:RB1-214
Abstract

As part of an investigation aimed at illuminating the possibilities and limits of the HOPE-fixation and paraffin-embedding technique we here describe a novel procedure which was developed in order to combine the benefits of the HOPE-technique with the capabilities of laser microdissection. The presented procedure avoids the need for amplification of template-RNA thus facilitating reliable and reproducible results. The excellent preservation of nucleic acids, proteins, and morphology in HOPE-fixed, paraffin-embedded tissues enhances the molecular applications available to date with materials acquired by laser microdissection when compared to formalin fixed, paraffin-embedded tissues, thus substantially extending the methodological panel in tissue based research.

Published
2006

53. Personalmarketing für die Generation Internet

Author

Grothe, Martin, primary, Becker, André, additional, Bender, Jens, additional, Buhl, Annekatrin, additional, Clasen, Marco, additional, Deglow, Sven, additional, Dépierre, Caroline, additional, Dietrich, Sebastian, additional, Eicher, Markus, additional, Erb, Oliver, additional, Fellinger, Christoph, additional, Grabmeier, Stephan, additional, Gunkel, Sina, additional, Heuzeroth, Corinna, additional, Komar, Edmund, additional, Kriegler, Wolf Reiner, additional, Kürn, Hans-Christoph, additional, Lindemann, Sara, additional, Mendius, Maximilian, additional, Preedy, Kara, additional, Quenzler, Alfred L. J., additional, Reif, Markus, additional, Scheller, Stefan, additional, Schmalstich, Maik, additional, Schmitz, Bernd, additional, Schweneke, Nina, additional, Sorg, Jürgen, additional, Stoisiek, Oliver, additional, Teetz, Thomas, additional, Uhrig, Andrea, additional, Ullah, Rubindro, additional, Wörle, Christiane, additional, Wurzer, Florian, additional, and Zabel, Peter, additional

Published
2014
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58. HOPE-BAL

Author

Marwitz, Sebastian, primary, Abdullah, Mahdi, additional, Vock, Christina, additional, Fine, Jay S., additional, Visvanathan, Sudha, additional, Gaede, Karoline I., additional, Hauber, Hans-Peter, additional, Zabel, Peter, additional, and Goldmann, Torsten, additional

Published
2011
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60. Myeloid-derived suppressor cells in the peripheral blood of cancer patients contain a subset of immature neutrophils with impaired migratory properties

Author

Brandau, Sven, primary, Trellakis, Sokratis, additional, Bruderek, Kirsten, additional, Schmaltz, Dominik, additional, Steller, Gabriele, additional, Elian, Motaz, additional, Suttmann, Henrik, additional, Schenck, Marcus, additional, Welling, Jürgen, additional, Zabel, Peter, additional, and Lang, Stephan, additional

Published
2010
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61. The TGF-beta-Pseudoreceptor BAMBI is strongly expressed in COPD lungs and regulated by nontypeable Haemophilus influenzae

Author

Drömann, Daniel, primary, Rupp, Jan, additional, Rohmann, Kristina, additional, Osbahr, Sinia, additional, Ulmer, Artur J, additional, Marwitz, Sebastian, additional, Röschmann, Kristina, additional, Abdullah, Mahdi, additional, Schultz, Holger, additional, Vollmer, Ekkehard, additional, Zabel, Peter, additional, Dalhoff, Klaus, additional, and Goldmann, Torsten, additional

Published
2010
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78. Tissue microarrays from HOPE-fixed specimens allow for enhanced high throughput molecular analyses in paraffin-embedded material

Author

Goldmann, Torsten, primary, Drömann, Daniel, additional, Marzouki, Marouane, additional, Schimmel, Udo, additional, Debel, Karsten, additional, Branscheid, Detlev, additional, Zeiser, Tobias, additional, Rupp, Jan, additional, Gerdes, Johannes, additional, Zabel, Peter, additional, and Vollmer, Ekkehard, additional

Published
2005
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92. Paraneoplastic syndromes in lung cancer.

Author

Heinemann, Stefanie, Zabel, Peter, and Hauber, Hans-Peter

Subjects
*PARANEOPLASTIC syndromes, *LUNG cancer, *TUMOR treatment, *CACHEXIA, *ASTHENIA, *METABOLIC syndrome
Abstract

Paraneoplastic syndromes are frequently found in lung cancer. They can be the first manifestation of disease or recurrence. Neuromuscular, vascular, haematological and metabolic syndromes, as well as syndromes involving the connective and skeletal tissues and skin can be distinguished. While some of the paraneoplastic syndromes are commonly observed (eg. Anemia, cachexia) others are rare disorders (eg. Opsoclonus-myoclonus, tylosis). This review focuses on the epidemiology, pathogenesis, clinical symptoms and treatement options of frequent and clinical important paraneoplastic syndromes. [ABSTRACT FROM AUTHOR]

Published
2008

94. LPS-induced mucin expression in human sinus mucosa can be attenuated by hCLCA inhibitors.

Author

Hauber, Hans-Peter, Goldmann, Torsten, Vollmer, Ekkehard, Wollenberg, Barbara, Hung, Hsiao-Ling, Levitt, Roy C., and Zabel, Peter

Abstract

Background: hCLCA1 is a member of the calcium-activated chloride channel family and is associated with disease-inducible mucus expression. Niflumic acid (NFA) and a closely related chemical structure are reported inhibitors of calcium-activated chloride channels and endotoxin-inducible mucus expression in the mouse. Therefore, we tested the hypothesis that hCLCA1 may be involved in lipopolysaccharide (LPS) induced mucin up-regulation in human airways. We also investigated the effect of NFA and MSI-2216 on LPS-induced mucin up-regulation.Materials and Methods : Explanted human airways and the muco-epidermoid cell line Calu-3 were stimulated with LPS. Different concentrations of NFA or MSI-2216 were added to LPS-stimulated airway mucosa and Calu-3 cells. Expression of hCLCA1 and MUC5AC mRNA and protein was quantified in human airways using real-time PCR and PAS staining. In addition, immunohistochemistry was performed for quantification of inflammatory cells (lymphocytes, monocytes, eosinophils, and neutrophils) in the submucosa of the airways. Expression of hCLCA1 protein in Calu-3 cells was analysed by FACS.Results: LPS significantly induced hCLCA1 and MUC5AC mRNA and protein expression in human airway mucosa (P < 0.05). NFA and MSI-2216 significantly decreased LPS-induced mucus expression in explanted airway mucosa in a dose-dependent manner (P < 0.05). In Calu-3 cells, LPS significantly increased hCLCA1 surface expression whereas intracellular expression was significantly decreased (P < 0.05). In Calu-3 cells, NFA and MSI-2216 also significantly reduced MUC5AC mRNA expression (P < 0.05).Conclusions: These data suggest that hCLCA1 may play a role in LPS-induced mucin expression in human airway mucosa. Calcium-activated chloride channel inhibitors significantly decreased LPS-induced mucus expression both ex vivo and in vitro . Therefore, blocking of hCLCA1 may offer a therapeutic approach to reduce bacterial-induced mucus hypersecretion. [ABSTRACT FROM PUBLISHER]

Published
2007
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95. Alveolar epithelial cells type II are major target cells for C. pneumoniae in chronic but not in acute respiratory infection

Author

Rupp, Jan, Droemann, Daniel, Goldmann, Torsten, Zabel, Peter, Solbach, Werner, Vollmer, Ekkehard, Branscheid, Detlev, Dalhoff, Klaus, and Maass, Matthias

Subjects
RESPIRATORY diseases, EPITHELIAL cells, OBSTRUCTIVE lung diseases, CHLAMYDIA
Abstract

Pulmonary presence of Chlamydia pneumoniae is associated with acute and chronic infections. We show that unapparent chlamydial infection in four out of 31 chronic obstructive pulmonary disease (COPD) patients (12.9%) is characterized by a significant increase in infected alveolar epithelial cells type II (18.2 ± 3.5% vs. 2.3 ± 0.9; IHC/ISH) compared to a newly established model of acute chlamydial infection (ACIM) in vital lung specimens from pulmonary lobectomy. Expression of cHSP60 demonstrated pathogen viability and virulence in the ACIM. We conclude that target cells differ in acute and chronic chlamydial infection and suggest the ACIM as a novel tool to analyze the host–pathogen-interactions in acute respiratory infections. [Copyright &y& Elsevier]

Published
2004
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97. HOPE-BAL: Improved Molecular Diagnostics by Application of a Novel Technique for Fixation and Paraffin Embedding

Author

Marwitz, Sebastian, Abdullah, Mahdi, Vock, Christina, Fine, Jay, Visvanathan, Sudha, Gaede, Karoline, Hauber, Hans-Peter, Zabel, Peter, and Goldmann, Torsten

Abstract

The bronchoalveolar lavage (BAL) and its cells have been widely used as a support for clinical diagnosis and as a versatile tool for research questions since many years. Because there are no sufficient possibilities of long-term storage, the authors explore in this study the utility of a new fixative for fixation and paraffin embedding of human lavage cells with the possibility of implementing standard molecular biology techniques. HOPE-fixed, paraffin-embedded BAL cells of patients with different lung diseases (asthma, chronic obstructive pulmonary diseases, tuberculosis, sarcoidosis, emphysema, and fibrosis) were subjected to immunohistochemistry, in situ hybridization, quantitative polymerase chain reaction, and transcription microarray analysis. Furthermore, two-dimensional gel electrophoresis was conducted to evaluate the range of possible applications for research, diagnostics, and further implementing in biobanks. The authors show, by targeting some exemplary molecules, the power of screening and validating HOPE-BAL for new biomarkers. The transforming growth factor β signaling pathway may play a central role in immunomodulation upon infection as well as asthma. Furthermore, haptoglobin was overexpressed in asthma and sarcoidosis. Because of the excellent preservation of nucleic acids, protein, and morphologic structures, HOPE-BAL is a step forward into enhanced molecular diagnostics and biobanking in pulmonary medicine.

Published
2011
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98. Myeloid‐derived suppressor cells in the peripheral blood of cancer patients contain a subset of immature neutrophils with impaired migratory properties

Author

Brandau, Sven, Trellakis, Sokratis, Bruderek, Kirsten, Schmaltz, Dominik, Steller, Gabriele, Elian, Motaz, Suttmann, Henrik, Schenck, Marcus, Welling, Jürgen, Zabel, Peter, and Lang, Stephan

Abstract

Altered myelopoiesis in patients with solid tumors results in increased numbers of immunosuppressive immature neutrophils with impaired migratory properties in the peripheral blood. In tumor‐bearing mice, immunosuppressive granulocytic and monocytic MDSC have been identified. The identity and function of MDSC in cancer patients are less clear and need further characterization. We analyzed the peripheral blood of 103 patients with HNC, lung cancer, or cancers of bladder and ureter. Based on sedimentation properties in density gradients, a subset of LD‐PMN was identified and analyzed. LD‐PMN were expanded in the peripheral blood of cancer patients, suppressed proliferation, and IFN‐γ production of polyclonally stimulated T cells and thus, qualify as human MDSC. Immunophenotyping and morphological analysis revealed the accumulation of immature PMN in the MDSC fraction. Neutrophilic MDSC showed altered surface marker expression, prolonged survival, and impaired effector functions when compared with conventional, mature PMN of regular density. MDSC displayed markedly reduced chemotaxis toward tumor‐conditioned medium and lacked expression of chemokine receptors CXCR1 and CXCR2, which are normally required for PMN extravasation from the bloodstream and subsequent tissue infiltration. Collectively, our data suggest the accumulation and persistence of long‐lived, immature granulocytic MDSC with T cell‐suppressive function and impaired migratory properties in the peripheral blood of cancer patients.

Published
2011
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100. Regional lung response to bronchodilator reversibility testing determined by electrical impedance tomography in chronic obstructive pulmonary disease.

Author

Vogt B, Zhao Z, Zabel P, Weiler N, and Frerichs I

Subjects
Administration, Inhalation, Aged, Aged, 80 and over, Electric Impedance, Female, Humans, Lung, Male, Middle Aged, Pulmonary Disease, Chronic Obstructive drug therapy, Pulmonary Disease, Chronic Obstructive physiopathology, Spirometry, Tomography, Treatment Outcome, Vital Capacity, Albuterol administration & dosage, Bronchodilator Agents administration & dosage, Pulmonary Disease, Chronic Obstructive diagnostic imaging
Abstract

Patients with obstructive lung diseases commonly undergo bronchodilator reversibility testing during examination of their pulmonary function by spirometry. A positive response is defined by an increase in forced expiratory volume in 1 s (FEV1). FEV1 is a rather nonspecific criterion not allowing the regional effects of bronchodilator to be assessed. We employed the imaging technique of electrical impedance tomography (EIT) to visualize the spatial and temporal ventilation distribution in 35 patients with chronic obstructive pulmonary disease at baseline and 5, 10, and 20 min after bronchodilator inhalation. EIT scanning was performed during tidal breathing and forced full expiration maneuver in parallel with spirometry. Ventilation distribution was determined by EIT by calculating the image pixel values of FEV1, forced vital capacity (FVC), tidal volume, peak flow, and mean forced expiratory flow between 25 and 75% of FVC. The global inhomogeneity indexes of each measure and histograms of pixel FEV1/FVC values were then determined to assess the bronchodilator effect on spatial ventilation distribution. Temporal ventilation distribution was analyzed from pixel values of times needed to exhale 75 and 90% of pixel FVC. Based on spirometric FEV1, significant bronchodilator response was found in 17 patients. These patients exhibited higher postbronchodilator values of all regional EIT-derived lung function measures in contrast to nonresponders. Ventilation distribution was inhomogeneous in both groups. Significant improvements were noted for spatial distribution of pixel FEV1 and tidal volume and temporal distribution in responders. By providing regional data, EIT might increase the diagnostic and prognostic information derived from reversibility testing., (Copyright © 2016 the American Physiological Society.)

Published
2016
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