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To solve the problems of poor mixing effect and poor sphericity for the silicon nitride particles in the dry granulation chamber, the influence of the inclination parameters for the open turbine typed axial‒radial combined structure on the mixing effect of silicon nitride particles prepared by dry method was studied. The mathematical model of Eulerian‒Eulerian gas‒solid two phase flow was established, and the physical model of the axial‒diameter combined structure granulation chamber was simplified. The computational fluid dynamics (CFD) method was used to analyze the volume distribution and velocity field of silicon nitride particles in granulation chamber in the axial structure under the different inclined angles. The sphericity of silicon nitride particles was tested by experiments, and the accuracy of numerical simulation results was verified. The numerical analysis results show that, when the inclination angle is 45°, the volume fraction of silicon nitride particles is 0.3~0.7 (accounting for 28%), showing the better circulating fluidity and the best mixing effect, compared with the inclination angle of 30° and 60°. The experimental results show that, the best average sphericity of the silicon nitride particles is 0.72 when the inclination angle of the axis‒diameter combined structure is 45°, which can effectively improve the mixing effect of silicon nitride particles.

Fatty acid oxidation (FAO) has been proven to be an accomplice in tumor progression. Carnitine palmitoyltransferase 1C (CPT1C), a rate-limiting enzyme in FAO, mainly functions to catalyze fatty acid carnitinylation and guarantee subsequent entry into the mitochondria for FAO in colorectal cancer (CRC). Gene expression data and clinical information extracted from The Cancer Genome Atlas (TCGA) database show significantly higher expression of CPT1C in patients with metastatic CRC ( P=0.005). Moreover, overexpression of CPT1C is correlated with worse relapse-free survival in CRC (HR 2.1, P=0.0006), while no statistical significance is indicated for CPT1A and CPT1B. Further experiments demonstrate that downregulation of CPT1C expression leads to a decrease in the FAO rate, suppression of cell proliferation, cell cycle arrest and repression of cell migration in CRC, whereas opposite results are obtained when CPT1C is overexpressed. Furthermore, an FAO inhibitor almost completely reverses the enhanced cell proliferation and migration induced by CPT1C overexpression. In addition, analysis of TCGA data illustrates a positive association between CPT1C expression and HIF1α level, suggesting that CPT1C is a transcriptional target of HIF1α. In conclusion, CPT1C overexpression indicates poor relapse-free survival of patients with CRC, and CPT1C is transcriptionally activated by HIF1α, thereby promoting the proliferation and migration of CRC cells.

The single event effect caused by space heavy ion radiation is one of the important factors affecting the safety and operation of spacecraft on orbit. In the research and evaluation of the frequency, spatial distribution and time characteristics of single event effects, linear energy transfer (LET) spectra of space radiation play an important role. On the Beidou navigation M15 and M16 satellites, a single event upset (SEU) and LET monitor was developed to obtain the upsets of the memory device and the LET spectra of space radiation which passes through the device. Through the measurement results from this monitor, the correlation between the device’s SEUs and the LET spectra could be studied.

This paper investigated the multi-port caving technology to improve the top coal recovery and caving efficiency of the longwall top coal caving(LTCC)in extra-thick seams.The 3D drawing experiments are designed based on the actual geological conditions of Panel 8222 in the Tashan Coal Mine.Results indicated that in the initial drawing stage, the drawing body of single-port caving is roughly in the shape of a funnel with a narrow bottom and a wide upper part.The drawing body of multi-port coal caving is characterized by a wide middle and narrow ends, the coal caving volume shows single-port>double-port>three-port.During the initial drawing stage, the larger the number of openings, the greater the slope of the top coal boundary line; the larger the disturbance range to the top coal; and the higher the lowest point of the top coal boundary line, the more low-level top coal is lost.The lowest point of the top coal boundary is basically at the center of the group of supports.When the advance of the panel is completed, the three caving ports release more medium and low-level top coal above and in front of the supports, the coal caving volume generally shows three ports>double ports>single port.

Tanshinone IIA has a potential therapeutic effect on cerebral ischemia/reperfusion injury (CIRI). In this study, tanshinone IIA was encapsulated in poly(lactic-co-glycolic acid)-block-poly (ethylene glycol)-carboxylic acid (PLGA-PEG-COOH) nanoparticles, and its therapeutic efficacy on CIRI was investigated. Morphology and dynamic light scattering analyses were performed to identify and optimize nano-formulations. A drug release test was conducted using the dialysis method. The cytotoxic effect of tanshinone IIA on human neuroblastoma cells (SH-SY5Y) and brain endothelial capillary cells (hCMEC/D3) was measured using the MTT assay. The protective effect of PLGA-PEG-COOH-encapsulated tanshinone IIA against CIRI was evaluated in oxygen and glucose deprivation/reoxygenation-induced SH-SY5Y/IR cells and middle cerebral artery occlusion (MCAO) rats. Results showed that PLGA-PEG-COOH-encapsulated tanshinone IIA promoted viability and inhibited apoptosis of SH-SY5Y/IR cells (P < 0.01). Moreover, PLGA-PEG-COOH-encapsulated tanshinone IIA facilitated the invasion of SH-SY5Y/IR cells and repressed inflammation in MCAO rats (P < 0.01). Noteworthy, PLGA-PEG-COOH-encapsulated tanshinone IIA combined with angiopep-2 peptide presented a better inhibitory effect on CIRI than tanshinone IIA alone (P < 0.01). Angiopep-2 peptide contributes to traversing blood–brain barrier by recognizing lipoprotein-related protein expressed in the brain capillary endothelial cells. In conclusion, PLGA-PEG-COOH-encapsulated tanshinone IIA plus angiopep-2 peptide holds promising therapeutic potential toward CIRI.

Objective To investigate the expression levels of thyroid transcription factor-1 （TTF-1） and CyclinD1 in ovarian cancer and analyze their relationship with lymphangiogenesis. Methods The ovarian cancer tissue samples were collected from 60 patients with ovarian cancer and corresponding ovarian tissue samples from 20 patients pathologically diagnosed with benign ovarian tumors and normal ovarian tissue were collected as the control group. Tissue sections were prepared for immunohistochemical staining of TTF-1， CyclinD1 and D2-40. The staining results of TTF-1 and CyclinD1 were evaluated based on semi-quantitative integration method. D2-40 staining results were assessed according to Weidner evaluation method. Lymphatic vessel density （LVD） was calculated. Results The positive expression rates of TTF-1 and CyclinD1 were 58% and 83% in the ovarian cancer tissues， which were higher than 5% and 10% in the normal cancer tissues（all P < 0.001）， respectively. The positive expression rates of TTF-1 and CyclinD1 were significantly increased in the ovarian cancer tissues with pathological stageⅢ-Ⅳ， lymph node metastasis， peritoneal metastasis， T3-T4 stage， N1 stage and M1 stage （all P < 0.05）. Age and histological type were not correlated with the positive expression rates of TTF-1 and CyclinD1 （all P > 0.05）. The LVD of ovarian cancer tissues with pathological stageⅢ-Ⅳ， lymph node metastasis and peritoneal metastasis was significantly increased （all P < 0.05）. The LVD of ovarian cancer tissues with positive expression of TTF-1 and CyclinD1 was significantly higher than that of ovarian cancer tissues with negative expression （both P < 0.05）. Conclusions TTF-1 and CyclinD1 are highly expressed in ovarian cancer tissues. Patients with severe ovarian cancer， lymphangiogenesis and metastasis have high expression of TTF-1 and CyclinD1， which may be potential new targets for ovarian cancer interventions.

Microdroplets have many unique features derived from the large surface-volume ratio and the ultrahigh electric field at the air-water interface, therefore they can remarkably accelerate various slow reactions and trigger reactions that won’t occur in bulk solutions. The size of atmospheric aerosols is similar to that of the microdroplets, so reactions performed in microdroplets and ambient conditions can act as good simulations of important atmospheric processes, which will provide a better understanding of the role of aerosols. Aldehydes are important oxidized species in the atmosphere derived from releases of human activities, such as vehicle emissions and biomass burning, which can generate volatile organic compounds (VOCs) and are highly related to saltrich aerosol formation through further oxidation. Studying of aldehydes’ hydration and oxidation reactions is very helpful for understanding the complex conversion process from VOCs to secondary organic aerosols (SOA). In this work, several aromatic aldehydes with different electron donating or withdrawing groups were studied by spraying their solutions into microdroplets, then directly characterizing them by online mass spectrometry. It showed that these aromatic aldehydes could be oxidized into carboxylic acids under the space-restricting condition of microdroplets, and the hydration reactions of aromatic aldehydes could be induced by carboxylate under the extreme pH environment and abundant reactive oxygen species provided by the air-water interface of microdroplets. Together, the products of oxidation and hydration reactions would combine to yield relatively unstable “carboxylate-aldehyde hydrate” complex ions. The lifetime of the ions was roughly estimated by conducting a collision-induced disassociation (CID) experiment and comparing it to the instrument running procedure, and the structure was checked using CID and a cross-forming reaction between two different aldehydes. This work revealed that the complex ions were potential intermediates of the atmospheric aldehyde oxidation reaction, providing a previously unknown pathway of atmospheric carboxylic acids and SOA. This result supported the view that atmospheric oxidation might take place in aerosols, such as fog and clouds, which was the missing part of SOA sources and matched the observations. However, unlike other liquid phase or gaseous simulation research of the oxidation process of aldehydes, the oxidation reaction would occur even without the participation of UVs or hydrogen peroxide addition in microdroplets, because the air-water interface could generate a large number of reactive oxygen species (ROS) like hydroxyl radicals and hydrogen peroxide, implicating that the air-water interface of aerosols might be an important place to perform many atmospheric reactions.

Probiotic fermented milk is an important carrier of probiotics. It enhances the nutritional and health properties of fermented milk by improving the balance of host intestinal microflora and promoting the metabolism of beneficial ingredients. This review expounds the auxiliary effects of probiotics fermented milk in improving intestinal function, regulating body immunity, assisting in treating metabolic diseases and improving mental system diseases, etc., in order to provide theoretical support for expanding the application of probiotics fermented milk.

Sichuan hilly area is located in southwest China, which have many hilly area, is a densely populated, economically active, frequent geological disasters area. In this paper, Neijiang City in the region is taken as the test sample. This article, which analyzes Land-Use and Land-Cover Changes (LUCC), meteorological and hydrological data from 2015 to 2020, takes Neijiang City in this region as the research object. We explored the relationship between geological disasters such as collapse and landslides that often occur in the natural ecological environment with LUCC and meteorological and hydrological factors, using GIS spatial analysis and statistical analysis methods. The research results indicate that geological disasters mainly occur in farmland areas. The density of geological disaster points is highest within urban areas, making them the most prone to geological disasters. Geological disasters are more densely distributed in the area within 600 to 800 m from the river. There is a strong correlation between the average rainfall amount and the probability of geological disasters occurring. This study provides a scientific basis for the geological disaster risk assessment, prevention and control in this region. It has certain reference significance for the ecological environment governance, land resources planning and management in other similar regions.

INTRODUCTION. Low back pain (LBP) is a common discomfort in young women during pregnancy and after childbirth. These female patients with lumbar disc herniation (LDH) have unique clinical manifestations, but few studies have been reported. AIM. To investigate the clinical characteristics of female patients with LDH after childbirth and to provide basis for rehabilitation andtreatment. MATERIAL AND METHODS. We collected the clinical data of 58 female patients with LDH, analyzed the clinical characteristics. We usedCT scan to determine the location and type of LDH, observed the dural sac of lumbar intervertebral disc, serum 25 hydroxyvitaminD (25[OH] D) levels were measured and compared with healthy women and pregnant women. RESULTS. Our results showed that according to the age distribution of patients with low back pain was the most common among 30-34years old, accounting for 43.1% of all patients. Patients with LDH accounted for the highest proportion of patients with LBP, up to 55.2%.L4-L5 segment was the most common part of LDH, accounting for 46.9%, followed by L5-S1 segment, accounting for 31.3%. Protrusionwas the most common type of LDH, accounting for 84.4%. Among 34 patients with LDH, 15 patients (46.9%) had dural sac compression.The serum 25(OH) D level of patients with LDH was 11.36±5.16 ng/ml, lower than that of healthy women (first control group, 15.22±3.42ng/ml, p=0.022), and pregnant women (second control group, 18.95±6.94 ng/ml, p

Objective： This study is to improve the sorting efficiency of string tomatoes, and solve its false detection and false detection. Methods: First, collect the image data set of string tomatoes, expand the data and improve the generalization performance of the model through data enhancement, and 3×3 convolution is replaced by improved SVM-MHSA layer. By replacing softmax classification function in MHSA with SVM classification function which is more suitable for string tomatoes, the detection accuracy of string tomatoes is enhanced. Secondly, the remaining 3×3 convolution is replaced by deep separable convolution to reduce the number of parameters and improve the operation efficiency. Finally, random correction linear unit is introduced to improve the convergence speed of network training. Results: the test results show that the improved tiny YOLOv5l model can effectively realize the string single fruit recognition and positioning and the whole string fruit counting. The detection frame loss rate is reduced from 1.48% to 1.34%, the target loss rate is reduced from 1.98% to 1.73%, the confidence loss is reduced by 1.4%, the accuracy is increased from 97.36% to 98.89%, and the recall rate is increased from 97.35% to 98.56%. Conclusion: The tiny YOLOv5l algorithm is more accurate and lightweight. It has a high recognition accuracy in the face of challenges such as occlusion, background interference, illumination change and virtualization, and provides accurate information on the location of single fruit and the quantity of the whole string of fruit for post natal string tomato sorters.

In order to solve the problems of low detection accuracy and less attention of vairous malicious traffic identification in traditional intrusion detection system (IDS) in the face of massive data mixed with various malicious traffic, an intrusion detection model based on capsule network CapIDS was proposed. In this model, the traffic to be measured was input into the capsule network in the form of capsules, and the characteristics of malicious traffic were extracted by using the dynamic routing mechanism to complete the identification of malicious traffic. At the same time, the structure of the capsule network was improved, which made the model have stronger generalization ability and improved the detection accuracy of malicious traffic. The experimental results show that the detection accuracy of the proposed model for malicious traffic has reached 99.50% on the NSL-KDD data set, which is better than that of other schemes.

Weathering steel is used more and more widely in various fields of production and life.A rust layer stabilization treatment on the surface of weathering steel before usage can enhance both material life extension and beautification，promoting the mutual coordination between weathering steel and its service environment.In this paper，the research status of surface stabilization treatment technique of rust layer on weathering steel was summarized，which mainly included the formation，composition，structure and protection mechanism of rust layer，especially the disadvantages of traditional technology and novel technology.Finally，the further development of the surface rust layer stabilization treatment was prospected.

Space radiation particles will cause a variety of radiation effects on orbiting satellites, such as single event effects, total ionizing dose, displacement damage, etc. In response to these space radiation effects, BeiDou Navigation satellites M15/M16 operating in medium earth orbit (MEO) developed the first integrated monitor for high-energy proton and particle radiation effects, based on space particle radiation detection technology. This payload realizes the joint observation of the high-energy proton environment, particle radiation linear energy transfer spectrum, and total radiation dose in the MEO. According to the detection data, research on the characteristics and laws of the space particle radiation effects can be carried out, and the problem of spacecraft reliability verification in orbit can be solved from the causal chain. In this article, we have introduced the high-energy proton detector (energy range 3–300 MeV), including technical indicators, working principles, instrument design, and ground calibration.

One-dimensional carbon materials were widely used in adsorption and purification, catalytic carriers, energy electronics and other fields because of their efficient mass transfer and diffusion efficiency, excellent mechanical properties and good chemical stability. Microwave heating met the requirements of current material processing and sustainable development by rapid treatment, improving energy efficiency and reducing equipment cost, and showed great application potential in the preparation of one-dimensional carbon materials. The development and application status of one-dimensional carbon materials and the unique advantages of microwave heating technology were introduced in this paper, the mechanism and characteristics of microwave heating were summarized, and the research and application status of microwave heating related to the preparation and performance optimization of biomass-based carbon nanotubes (CNTs), carbon nanofibers (CNFs) and polyacrylonitrile-based carbon nanofibers (PAN-based CNFs) were summarized. In addition, the action mode of microwave heating on biomass-based one-dimensional carbon materials during carbonization and the changes of structure and properties of PAN-based carbon fibers during microwave heating were emphatically analyzed. Finally, it was pointed out that accurate and reliable temperature measurement tools and corresponding microscopic characterization methods should be developed, the simulation and modeling tools should be used to innovatively design novel microwave reators, and the understanding of the interaction mechanism between materials and microwave through scientific research practice and basic research should be deepened, and to promote the extensive and in-depth application of microwave heating technology in the field of material processing.

Taking lithium battery of new energy vehicles as the research object,an echo state network (ESN) model is established to predict the state of charge (SOC) of the vehicle's lithium battery. The cross-validation method is used to optimize the parameters of the ESN to solve difficulty to select arameters of the model. The echo state network is trained by recursive least squares method with forgetting factors to calculate the output weight matrix so as to improve the adaptability and accuracy of the network.The feasibility of the prediction algorithm is further analyzed and verified by the model simulation. The research further analyzes and compares the predicted SOC of the established ESN model, the BP neural network algorithm and radial basis function (RBF) network algorithm under UDDS, US06 and NYCC. The research results show that the established echo state network model is superior to the BP algorithm and RBF algorithm in estimating the performance and effect of lithium-ion battery SOC evaluation. Using ESN model to predict SOC has a good application prospect and can provide a reference for long-term and effective SOC prediction of the lithium battery.

Krüppel-like factor 7 (KLF7) is a negative regulator of preadipocyte differentiation. Our previous KLF7 ChIP-seq analysis showed that the binding motif of PU.1 was found among the KLF7 binding peaks, indicating that an interaction between KLF7 and PU.1 at preadipocyte gene promoters and other regulatory elements might be common. Here, Co-IP and FRET assays are used to confirm that PU.1 can directly bind to KLF7 and enhance the transcription activity of cyclin-dependent kinase inhibitor 3 ( CDKN3), which is a downstream target gene of KLF7. We show that the PU.1 expression level is decreased during preadipocyte differentiation. Furthermore, PU.1 overexpression and knockdown experiments reveal that PU.1 negatively regulates chicken preadipocyte differentiation, as evidenced by appropriate changes in lipid droplet accumulation and altered expressions of PPARγ, FAS, and PLIN. In addition, PU.1 overexpression promotes preadipocyte proliferation, while knockdown of PU. 1 inhibits preadipocyte proliferation. We further demonstrate that PU.1 inhibits differentiation and promotes proliferation in preadipocytes, in part by directly interacting with KLF7.

In order to summarize the current status and evolution of surimi research in China and globally, 944 and 1 082 research paper published in the period of 2009 to 2021 were retrieved using the keywords: yumi (in Chinee) and surimi from the China national knowledge infrastructure (CNKI) and Web of Science (WOS) databases and were test mined for the number of research papers, authors, countries, institutions and journals using CiteSpace and the online bibliometric analysis platform. Moreover, data visual analysis was carried out by plotting keyword clustering, time line and burstness maps. It was found that there has been increasing interest in surimi research. The number of surimi research papers indexed in CNKI and WOS has shown an increasing trend in the period from 2009 to 2021, and most papers were published in the top journals in the food field. China has published 441 research papers in English, ranking first in the world, accounting for 37.99% of the world’s total, followed by Thailand, the United States and Japan. The close cooperation between authors and research institutions in different regions and countries has formed an international academic community. The research content has shifted from the macroscopic field to the microscopic field, and the research hotspots have changed from processing technology and surimi quality to preservation and antifreezers. At present, “protein” “myosin” and “secondary structure” have become new keywords and the research content has reached the molecular level. The time zone analysis showed that the keywords “sensory evaluation” and “quality” span the entire research period and have become the cornerstones of surimi research. In conclusion, surimi research is still in an active stage and will have a bright future.

VOCs are the main source of indoor environmental pollution. Bodalal established a mathematical model of VOCs emission characteristics, which provided a reference for many scholars to study the diffusion of VOCs. However, there are some logical errors in the process of mathematical derivation, which make the model difficult to understand. In the paper, first, the reasons for the error of model derivation are pointed out in detail. Second, using Laplace–Carson transform and generalised Heaviside theorem, the correct derivation process is given. Finally, a simple method for calculating the diffusion coefficient is presented and its application is given. This paper provides convenience for people to use this model in related research.

Spinal cord injury (SCI) is a severe central nervous system disease, which may cause serious locomotor deficit. Acacetin is a flavone that possesses antioxidant and anti-inflammatory effects in different human diseases. The main purpose of this study was to explore whether acacetin ameliorates SCI in mice. A model of SCI was established in C57BL/6 mice. The Basso Mouse Scale (BMS) score, BMS subscore, mechanical hypersensitivity, and thermal hypersensitivity of mice were tested for determining the motor function. Immunofluorescence staining was utilized to detect NeuN, GFAP, and Iba-1 levels in spinal cord tissues. ELISA was utilized to assess the contents of proinflammatory factors such as interleukin (IL)-1β, IL-18, and tumor necrosis factor-alpha (TNF-α) in spinal cord tissues. The levels of oxidative stress markers, reactive oxygen species, thiobarbituric acid-reactive substances, superoxide dismutase, catalase, glutathione peroxidase, and glutathione were detected using their corresponding kits. Western blot was employed for estimating the levels of heme oxygenase 1 (HO-1), nuclear factor E2-related factor 2 (Nrf2), and Kelch-like ECH-associated protein 1 (Keap-1). In this study, acacetin treatment recovered the motor function in SCI mice. Acacetin improved neuron integrity and repressed glial cell activation in the spinal cord tissues of SCI mice. Furthermore, acacetin administration reduced the SCI-induced high concentrations of IL-1β, IL-18, and TNF-α, as well as inhibited oxidative stress in SCI mice. Moreover, acacetin activated HO-1/Nrf2 pathway in SCI mice. The neuroprotective effects of acacetin against SCI were reversed by Nrf2 inhibitor. Overall, acacetin alleviated neuroinflammation and oxidative stress injury by activating the Nrf2/HO-1 signaling pathway in the mouse models of SCI.

Objective： A tomato weight detection method based on image processing was established to realize non-contact tomato weight detection. Methods： The binary image of tomato was obtained through image processing. The geometric features of tomato were extracted by pixel statistics and minimum circumscribed rectangle method, and correlation analysis was made between the characteristics and the real value of tomato weight, then the regression model of tomato weight detection with geometric features as parameters was established. Results： Compared with the real size of tomato, the measurement error of transverse and longitudinal diameter of Tomato by minimum external rectangle method was less than 3%. In addition to fruit shape index, other geometric characteristics were linearly correlated with tomato fruit weight, and the correlation between positive characteristics and fruit weight was more significant. Three types of 20 models were established for prediction and evaluation. The multiple regression model with the parameters of tomato front projection area and perimeter, projection area of a side image and tomato transverse diameter had the highest accuracy, the regression coefficient was 0.962, the average relative error of detection value was 0.673%, and the average absolute error was 1.425 g. Conclusion： The model is suitable for the weight detection of tomatoes and other fruits or articles with similar axisymmetric shape characteristics.

Objective: This study aimed to research and develop puffed blueberry dregs indica rice and fruit snack food. Methods: Using indica rice flour and blueberry residue powder as raw materials, three experimental factors including moisture content, extrusion temperature and screw speed were selected, and the expansion degree, anthocyanin content and sensory score were taken as comprehensive indexes. The expansion process was optimized by single factor and orthogonal test, and the antioxidant activity of the expanded products was studied. Results: The optimum process parameters were as follows: the moisture content of the material was 16%, the temperature of the four extrusion zones were 50—80—110—130 ℃ and the screw speed was 160 r/min. The indica rice fruit with blueberry residue obtained under this process has the highest comprehensive score of 62.30±0.51. The results of antioxidant test in vitro showed that compared with puffed indica rice fruit, the scavenging rates of DPPH and ABTS by blueberry residue indica rice fruit increased by 60.19% and 73.67% respectively. Conclusion: the optimal processing parameters of indica rice fruit with blueberry residue were obtained, and the product also showed good antioxidant properties.

Bran is a by-product of naked barley mainly including seed coat, germ, aleurone layer and a small amount of starchy endosperm. It is not only rich in nutrients, but also has functional components such as arabinoxylan, β-glucan, phenolic compounds and phytosterol. Besides, it has a variety of physiological activities such as hypoglycemic effect, antilipemic function, antioxidant capacity and intestinal tract regulation, and can meet the demand for a healthy diet. However, rough taste and poor digestibility result in its insufficient processing and utilization, and limit the development of its product. With the advocacy of low-carbon and innovative ideas, there has been an increasing number of related researches on naked barley bran in recent years. The oil, dietary fiber, fermentation product and related processing technologies based on naked barley bran have developed continually, which has stimulated the potential for its efficient utilization. To provide a theoretical reference for breaking through the key technology of deep processing and accelerating the commercialization of high-quality products, the research process of functional components and product development of naked barley bran have been reviewed.

Cervical cancer is the fourth most common cancer and the fourth leading cause of cancer death in women. Human papillomavirus (HPV16) E6/E7 heterogenous expression in C33A cells increased the mRNA and protein levels of KIF2A, while siRNA deletion of endogenous E6/E7 reduced the mRNA and protein levels of KIF2A in SiHa cells. KIF2A promoted cell migration and invasion, and regulated the expression of epithelial–mesenchymal transition-related proteins in C33A and SiHa cells. The exogenous expression of E6/E7 in C33A cells increased the phosphorylation of Akt, ERK, and JNK. However, Akt (API-2) and ERK (PD98059) inhibitors had no effect on the increase in KIF2A expression induced by E6/E7, while JNK inhibitors (JNK-IN-8 and SP600125) blocked the increase in KIF2A expression induced by E6/E7. The exogenous expression of E6/E7 increased the levels of transcription factor c-Jun, which is the classic substrate of JNK. Knockdown of c-Jun reduced the increase in KIF2A expression induced by E6/E7. In summary, KIF2A plays a key role in the motility and metastasis of cervical cancer. HPV16 E6/E7 can increase the levels of transcription factor c-Jun by activating the JNK signal, thereby up-regulating the transcriptional expression of KIF2A.

Objective: A high-throughput detection method for Cu2+ was developed with a well designed water-soluble fluorescent probe（HWEHH） and the microplate reader. Methods: The specificity of the fluorescent probe for Cu2+ determination was studied with the selectivity experiment. The linear range and detection limit of the probe for Cu2+ determination was detected with titration experiment, and the accuracy of the probe for the detection of Cu2+ in water was studied with sample spiked recovery method. Results: The fluorescent probe was able to specifically detect Cu2+. When the concentration of peptide probe was 2 μmol/L, the linear range of Cu2+ detection was 0~1.5 μmol/L. The linear equation was Y=-1 135.085 7X+2 048.187 6, and the detection limit was 0.036 μmol/L. The binding constant of HWEHH-Cu2+ complex was 1.3×106 mol/L. The linear range of high-throughput detection method was 0~1.5 μmol/L, and the recovery rate was 93.6%~101.9%. Conclusion: HWEHH peptide fluorescent probe can be used for high-throughput detection of Cu2+ in aqueous solution.

In order to provide a comprehensive reference for the study of carbon dioxide displacement of coalbed methane, this paper explores the influence of different influencing factors on coal adsorption capacity, analyzes the gas migration and adsorption principle in coal bed, as well as the difference and relationship between different adsorption models, and introduces the pilot test and application of CO2-enhanced coalbed methane recovery in different countries. Using VOSviewer software and CNKI metrological visualization analysis function, this paper analyzes the annual publication trend and keyword time trend of CO2-ECBM related literature in CNKI and Web of Science databases, and discusses the problems existing in CO2-ECBM. The results show that coal rank and pore structure, temperature and pressure, moisture content and other factors can affect coal adsorption capacity in different forms. Gas migration in coal seam is a complex system composed of adsorption/desorption-diffusion-seepage. The best adsorption model should be considered in studying coal adsorption gas. Based on VOSviewer analysis, CO2-ECBM research is a hot topic in China. The research direction has gradually shifted from macroscopic geological storage evaluation to reveal the mechanism of coal storage and displacement gas from microscopic or molecular levels such as pore structure and composition of coal, and multi-field coupling simulation of CO2-ECBM.

CD70 is overexpressed in a variety of solid and hematological tumors and plays a role in tumor proliferation and evasion of immune surveillance. Targeting and blocking its binding to the receptor CD27 have the potential to treat CD70-dependent tumors. To generate novel CD70 blocking agents, we screen a human CD70-immunized camel VHH phage display library and isolate two blocking nanobodies against human CD70 targeting different epitopes. Upon enrichment by three rounds of biopanning, two strategies are employed to identify CD70 blockers. One named affinity selection is used for detecting clones with CD70 binding by conventional PE-ELISA. However, no clone with a blocking effect is obtained from 188 enriched clones by this method. The alternative strategy named competitive selection is based on the inhibiting capacity of CD70-CD27 binding by enriched VHHs. By this method, two clones, Nb-2B3 and Nb-3B6, with strong blocking capacity are obtained from 20 enriched VHHs, suggesting the efficiency of this strategy. Furthermore, Nb-2B3 and Nb-3B6 specifically bind to CD70-positive SKOV3 and Raji cells at low concentrations. Meanwhile, Nb-2B3 has no competitive effect on the binding of Nb-3B6 to CD70, and vice versa, indicating that they target two different epitopes on CD70. Our data show that nanobodies Nb-2B3 and Nb-3B6 are potential attractive theranostic agents for CD70-expressing cancers.

AIM. To study the clinical effect of body composition-based lifestyle intervention in patients with polycystic ovary syndrome (PCOS). MATERIAL AND METHODS. In the period of November, 2020 – July, 2021, 91 patients diagnosed with PCOS in the First People's Hospital of Urumqi were prospectively selected and divided into a control group (46 cases) and an experimental group (45 cases) according to random number method. The patients in the control group were treated with Drospirenone and Ethinylestradiol tablets (II), while the patients in the experimental group were treated with Drospirenone and Ethinylestradiol tablets (II) and lifestyle adjustment based on body composition. The changes of the indices before and after 3 months of treatment were compared between the two groups. RESULTS. The indices of PCOS patients in the two groups after treatment (except 1 basal metabolic rate and muscle weight, 2. FSH and 3. HDL) were decreased, and the decrease in the experimental group was more significant (P < 0.05). Patients in the experimental group had significantly increased spontaneous ovulation and pregnancy rate / Conducive to ovulation and pregnancy. CONCLUSION. In PCOS patients, the combination of Drospirenone and Ethinylestradiol tablets (II) and lifestyle intervention based on human body components can improve reproductive hormone, glucose metabolism and insulin resistance more effectively, which is beneficial to spontaneous ovulation and pregnancy, and reduce the weight of patients.

With the continuous increase of renewable energy permeability， the randomness of new energy and the benefit game between different operators bring a great challenge to the economic dispatch of power system. For safety and economy improvement of microgrid system， the paper proposes an autonomous economic dispatch method for multi-microgird systems considering the complementarity between wind solar power. With the goal of optimal cost for operation economy of multi-microgrid and taking account of unit regulation cost under multiple scenarios， a typical scenario generation method of wind and photovoltaic power based on Copula theory is proposed. Analytical target cascading （ATC） is used to establish a two-layer optimization model to optimize microgrid and distribution networks autonomously and independently. The IEEE 33 bus system is taken as an example to compare the economic efficiencies of multi-microgrid systems that take account of wind-solar power correlation and multi-microgrid systems that take account of random scenarios only. Furthermore， the economic efficiencies are compared with that of the centralized dispatching method. It is verified that the dispatching method taking account of the complementarity between wind and solar power can improve system economy and enhance system robustness under extreme scenarios.

Objective To investigate the effect of cognitive⁃motor dual⁃task on gait automaticity in patients with Parkinson's disease (PD). Methods A total of 45 patients with primary PD in Tianjin Huanhu Hospital were selected from June 2020 to December 2021. Cognitive single task, motor single task and cognitive⁃motor dual⁃task in the "off" and "on" states of dopaminergic medication were performed. The correct number of subtractions was recorded to measure cognitive performance. A three⁃dimensional gait analyzer was used to extract the parameters of stride length and gait velocity, and the dual⁃task cost (DTC) was calculated to measure gait automaticity. Results Compared with single task, patients with PD showed slower gait velocity (t=11.909, P=0.000; t=11.621, P=0.000), shortened stride length (t=9.027, P=0.000; t=9.991, P=0.000), while there was no significant difference in the correct number of subtraction (t=0.651, P=0.519; t=1.205, P=0.234) during both "off" and "on" states. In patients with PD, the DTC of gait velocity (Z=⁃4.148, P=0.000) and stride length (Z=⁃4.114, P=0.000) in the "off" state was higher than that in the "on" state, while the DTC for subtraction had no significant difference (Z=⁃1.005, P=0.315). The DTC of gait velocity in the "off" and "on" states of patients with PD was negatively correlated with the Mini⁃Mental State Examination (MMSE) score (rs=⁃0.474, P=0.000; rs=⁃0.461, P=0.000), but positively correlated with Unified Parkinson's Disease Rating Scale Ⅲ (UPDRS Ⅲ) score (rs=0.446, P=0.002; rs=0.428, P=0.003). Conclusions Patients with PD have a significant decrease in stride length and gait velocity, and higher risk of falling when performing dual⁃task. Dopaminergic medication can improve the gait automaticity of patients, and cognitive decline may lead to gait automaticity disorder in patients with PD.

Objective: To study the effects of microbial nitrification inhibitors on the formation and changes of flavors in air-dried sausages. Methods: Four groups of air-dried sausages were designed: MNI group: 0.05% of microbial nitrosation inhibitors （MNI） were added; MNIP group: 0.05% MNI and an inoculation of PRO-MIX5 commercial starter （Staphylococcus xylose, Lactobacillus sake, and L. plantarum） were added; FBFAP group: fermented beef flavorings and compound antioxidants were added, and the sausages were inoculated with the PRO-MIX5 commercial starter; and CK group: blank control （CK）. The flavor of air-dried sausages was determined by an amino acid analyzer, Heracles iii ultra-fast gas phase electronic nose, electronic tongue and gas chromatography-mass spectrometry （GC-MS）. Results: Glutamate, alanine, taurine and carnosine are the primary free amino acids in the four groups of air-dried sausages. The addition of MNIP promoted the production of fresh amino acids and increased the total amount of free amino acids. Glutamate and alanine, two umami and sweet amino acids, contributed the most to the taste of air-dried sausages, and the four groups of air-dried sausages were primarily umami and sweet. There were differences in flavor and taste among the four groups. The electronic nose and electronic tongue principal component analysis （PCA） could distinguish between them. The flavors and taste of the MNI and MNIP groups were similar, and the taste of FBFAP group and CK were similar. The addition of MNI and MNIP improved the umami of air-dried sausage and reduced its saltiness and bitterness. Ethyl hexanoate and ethyl isovalerate were the characteristic flavors of the MNIP group. Linalool was the characteristic flavor of the MNI group, while decanal and ethyl isovalerate were the characteristic flavors of the FBFAP group. Conclusion: The addition of MNI alone or the synergistic effect of MNI and PRO-MIX5 promotes the formation of flavor in the air-dried sausages, indicating that MNI shows great promise at promoting the formation of flavor in air-dried sausages.

With the continuous expansion of Grain Wheat processing capacity and the increase of bulk grain vehicle transportation volume year by year, it needs to promote the use of bulk grain unloading system based on hydraulic turnover in factories in order to promote the safety, environmental protection, efficiency and economy of bulk wheat unloading. Taking bulk grain unloading system as the research and analysis object, this paper compared and analyzed the data before and after the application of the system, and designed different experimental groups for the fan opening to explore the influence of the fan on the dust concentration. The results showed that the system can significantly improve the grain unloading efficiency per capita, the investment return period was short, the system design was safe and reliable, and the dust concentration during grain unloading decreased significantly with the increase of fan opening (P

In this work, we design and implement a user-friendly, AIempowered, auto-pipelining data analysis system for biological macromolecule crystallography. It consists of four modules, (1) data reduction that generates reference reflection files from X-ray diffraction images, (2) structure prediction via database-querying or AlphaFold/OpenFold real-time prediction, (3) molecular replacement and (4) module building and refinement. This data analysis system, currently at Work-In-Progress stage, is based on and developed for High Energy Photon Source initially, aiming at automatic, intelligent, and highefficiency software and will be open-source for academic research.

This study delves into senior tourists’ engagement behaviour in social networking site advertisements (SNSA) and the underlying factors influencing their behaviour. Using a qualitative research approach, 19 senior tourists with prior SNSA engagement experience were interviewed. The findings reveal three distinct patterns in senior tourists’ SNSA engagement: 1) Proactive Engagement, 2) Passive Engagement, 3) Positive Consciousness Driven Engagement. These insights provide valuable guidance for the hospitality and tourism industry. By understanding the multifaceted nature of senior tourists’ engagement with SNSA, industry participants can tailor their marketing strategies to better target and engage this demographic.

Objective To investigate the effect of over-activation of mTORC1 on mouse intestinal epithelial homeostasis by conditional knockout of Depdc5. Methods Mice were divided into Depdc5flox/flox group (control group), Depdc5flox/flox:Villin-Cre group (IKO group), rapamycin-Depdc5flox/flox group (rap-Ctrl group) rapamycin-Depdc5flox/flox:Villin-Cre group (rap-IKO group). Western blot was used to detect the protein expression of DEPDC5 and the phosphorylation S6 (PS6) which is the downstream molecule of mTORC1 in the intestinal epithelium. qPCR was applied to detect the mRNA levels of Dclk1, Trpm5 and Muc2 in intestinal epithelial cells (IECs). Hematoxylin-eosin (HE) staining was used to observe the intestinal morphology changes. Immunohistochemical (IHC) staining and alcian blue staining were used to detect the number of tuft cells, Paneth cells, proliferative cells and goblet cells. Results The protein expression of PS6 in the small intestine and colon from the mice of IKO group was obviously up-regulated than that of the control group (0.957±0.028 vs. 0.598±0.041) (P＜0.05). The mRNA levels of Dclk1, Trpm5, and Muc2 were significantly decreased in the IKO group than those in the control group (P＜0.05). The numbers of tuft cells, Paneth cells and goblet cells in the IKO group were all significantly reduced than that in the control group. Nevertheless, the crypt depth in the IKO group was significantly higher than that of the control group (P＜0.05), and the number of proliferative cells was also increased in the IKO group(P＜0.05). Conclusions The over-activation of mTORC1 inhibits the differentiation of IECs and impairs the homeostasis of intestinal epithelial.

Polyether imide (PEI) flat ultrafiltration membrane was prepared by immersion precipitation phase inversion method. The effects of polymer PEI concentration and additive polyvinylpyrrolidone (PVP) concentration on the structure and properties of membrane were investigated. In order to improve the solvent resistance of ultrafiltration membrane, hexamethylenediamine (HDA) was used for chemical crosslinking, and the difference of membrane performance under different crosslinking time was studied. The results show that the flux of the crosslinked membrane is effectively improved without reducing the membrane separation performance compared with the uncrosslinked ultrafiltration membrane. Under the operating pressure of 0.1 MPa, the flux of pure water is increased from 667.1 L/(m2·h) to 1126.1 L/(m2·h), while the rejection rate of 1.0 g/L BSA solution remains above 97%. After soaking in six different solvents for 48 h, it was found that the uncrosslinked membrane is completely dissolved in N, N-dimethylformamide (DMF), while the crosslinked membrane still maintains a good membrane morphology with an adsorption capacity of 0.86 g/g. The adsorption capacity in ethanol, isopropanol, n-hexane and 1 mol/L HCl solution is smaller than that of the uncrosslinked membrane, but is increased in 1 mol/L NaOH solution, which indicates that the prepared crosslinked membrane has good solvent resistance and strong acid resistance, but the strong alkali resistance becomes worse.

Aimed at the shortcomings of existing methods in identifying evidence conflicts, in combination with traditional evidence conflicts, an evidence conflict identification and adjustment method is proposed. First, the weighted average of traditional evidence conflicts is used as the identification index in this method. Then, the credibility and uncertainty are introduced to determine the weight coefficients in two situations based on whether it is a conflict between different evidences. After that, the identified evidence conflicts are adjusted in combination with the traditional evidence conflicts and Jousselme information distance. This method is based on the traditional evidence conflicts, which ensures the representativeness of the identification index and the authenticity of the identification results. In addition, the weight coefficients comprehensively consider the credibility within and between the evidence, which is also more representative. Finally, the reduction degree and the average deviation are selected to analysis and verify the proposed method. The results show that the proposed method has a high recognition accuracy and can effectively adjust evidence conflicts.

In this review, the cultivation environment, research and development status, nutritional composition, health effect and application in food and medicine of Changzhi region characteristic export products“Qinzhou Huang”millet were summarized,and its development prospects, development and utilization were prospected.

ObjectiveTo analyze the Nogo-P3 component of event-related potential （ERP） in the process of visual acuity processing， to provide electrophysiological evidence for objective evaluation of visual acuity.MethodsTwenty-six subjects with no other ocular diseases except for ametropia were recruited， and all subjects had uncorrected visual acuity both eyes 1/10 （evaluated using Monoyer chart）. Block letter E with different visual angles and directions were used as graphic stimuli. The Go/Nogo paradigm was used for ERP studies. The visual angle of Go stimulation angle was 1°15′， Nogo stimuli were 1°15′， 55′， 24′ and 15′. The visual acuity test was performed on each of the two naked eyes separately in all subjects， and the characteristics of the Nogo-P3 component were analyzed.ResultsThe latency of Nogo-P3 showed no difference between the stimuli of 1°15′ and 55′， and between Nogo stimulation angle 24′ and 15′. There was significant difference between Nogo stimulation angle 1°15′ and 24′， and between Nogo stimulation angle 1°15′ and 15′ （P

Objective: This study aimed to explore the regulatory effect of Lactarius hatsudake polysaccharide on intestinal flora. Methods: LHP was extracted from L. hatsudake by hot water. LHP-1 and LHP-2 were further purified and characterized. The effects of LHPs on body weight and intestinal microbiome composition were studied in antibiotic-induced mice. Results: LHP-1 was mainly composed of mannose, glucose and galactose. The molar mass ratio of LHP-1 was 3.44∶61.10∶1.22. LHP-2 is mainly composed of mannose and glucose, with a molar mass ratio of 3.84∶153.00. It has a "neuronal" structure and a chain structure region. LHP intervention can restore the structure and diversity of intestinal flora by increasing beneficial bacteria such as Bacteroides and Akkermansia and reducing harmful bacteria such Enterococcus and Brevundimonas. In particular, the increase of the beneficial bacteria A. muciniphila indicates the great potential of LHP in the regulation of human intestinal flora. Conclusion: Polysaccharide from Lactarius hatsudake can improve intestinal metabolism as potential prebiotics.

Objective To compare the clinical characteristics between intrasuprasellar and suprasellar Rathke cleft cyst (RCC), and investigate the therapeutic efficacy of endoscopy for suprasellar RCC. Methods We retrospectively analyzed 61 intrasuprasellar and 15 suprasellar RCC treated by endoscopic endonasal approach (EEA) (standard or extended) in our hospital between January 2013 and August 2018. Their clinical manifestations, surgical conditions and outcomes were compared between the 2 groups. Results The cyst size was significantly smaller in suprasellar RCC than those in intrasuprasellar region (13.1±2.0 vs 17.5±4.2 mm, P < 0.001). The patients with suprasellar RCC were more commonly presented with visual loss, visual field defect and hyperprolactinemia than those with intrasuprasellar RCC (60.0% vs 27.9%; 66.7% vs 37.7%, P < 0.05). The duration of surgery and postoperative hospital stay were significantly longer in the suprasellar RCC group than the intrasuprasellar group (136±21 vs 106±33 min, P=0.001; 11.7±2.7 vs 5.8±3.1 d, both P < 0.001). The incidences of new- onset diabetes insipidus, intracranial infection and hyponatremia were obviously higher in the former group than the latter group (33.3% vs 8.2%, 20.0% vs 1.6%, 46.7% vs 14.8%, all P < 0.05). During the follow-up of 50.4±19.8 months, higher recurrence rate was observed in the suprasellar RCC group than the intrasuprasellar RCC group (26.7% vs 6.6%, P < 0.05). Conclusion Compared with intrasuprasellar RCC, suprasellar RCC, even though in smaller size, may present higher incidences of preoperative visual loss and field defect and hyperprolactinemia before operation, and more common new-onset diabetes insipidus, meningitis and hyponatremia postoperatively, as well as longer surgical period and hospital stay. Extended EEA is an effective and safe approach for the treatment of suprasellar RCC.

The aim of this study was to identify the impacts of different driving factors on terrestrial ecosystem evolution. The Yellow River Basin was selected as the study area, of which terrestrial ecosystem was deeply affected by climatic change and human activities. We constructed four scenarios (including without any impacts, affected by climate change, by human activities and by both impacts), and the discrepancies between them reflected the impacts of climate change or human activities. Based on this, the future land use simulation model was used to simulate the land use distribution under the four scenarios, and then, the ecosystem services values (ESV) and landscape patterns index were evaluated. The results indicated that affected by climate change during 1995–2015, the Mean Patch Area of the forestland decreased by 0.19% and the landscape patterns became fragmented. Meanwhile, the total ESV decreased by 0.03 billion dollars and the ecosystem regulation services were weakened. Under the influences of human activities, the Contagion index decreased by 1.71% and the landscape patterns became dispersed. Simultaneously, the total ESV increased by 0.56 billion dollars, but the function tends to be unitary. In addition, these effects showed great spatial heterogeneity. This study provides scientific support for ecological protection in the Yellow River Basin.

Objective To investigate the causes and risk factors of recent death (within 30 d postoperatively) of patients with sellar tumors after endoscopic transsphenoidal surgery (ETS). Methods The clinical data of the patients with sellar tumors after ETS in our department from January 2011 to March 2021 were collected and retrospectively analyzed. Death cases were collected. Univariate analysis and multivariate logistic regression analysis were used to determine the independent risk factors for postoperative death. Results In the past 10 years, there were 1 635 cases of ETS surgery in our department, including 1 470 cases of endoscopic transsphenoidal approach (ETA), and 165 cases of extend endoscopic transsphenoidal approach (EETA). Nine patients died recently after operation, with a mortality of 0.55% (9/1 635). The causes of death included: ① hemorrhage in the operative area in 3 cases (subarachnoid hemorrhage in 1 case and residual tumor hemorrhage in the operative area in 2 cases); ② infection in 3 cases (2 cases of meningitis and 1 case of blood-borne infection); ③ hypothalamus injury in 1 case; ④ vascular injury in 1 case; ⑤ epileptic status in 1 case. Univariate analysis showed that diameter >3 cm, tough consistency, large amount of intraoperative blood loss, intraoperative cerebrospinal fluid (CSF) leakage, persistent postoperative CSF leakage, remnant tumor, and treatment of EETA may be associated with postoperative death after ETS (all P < 0.05). Multivariate logistic regression analysis indicated that intraoperative CSF leakage was the only independent risk factor. Conclusion The main causes of recent death of insellar tumor patients after ETS are hemorrhage in surgical area, meningitis and blood-borne infection. Those with intraoperative CSF leakage are more likely to die.

The potential signal generated in the process of coal rock deformation and failure can better characterize the damage evolution process of coal rock, and has a good application prospect in the field of coal rock dynamic disaster monitoring and early warning. At present, most of the researches focus on the potential characteristics and laws of the same type of coal rock failure, and there is a lack of systematic research on the comparative analysis of potential characteristics of different types of coal rocks. At the same time, there are few comparative studies on the effect of different lithology coal rock structure failure process and components on the surface potential signal generation mechanism at the micro level. In order to deeply study the response law and difference of potential signal of coal and rock with different lithology, four kinds of samples, graphite, raw coal, sandstone and granite, are selected for uniaxial loading and the potential signals generated in the process of damage and failure are collected synchronously. The variation characteristics of potential signals of four samples under loading and failure are analyzed. The results show that the potential signal value of graphite sample is relatively low, and the potential signal fluctuates greatly during the crack damage and unstable crack propagation stages. The fluctuation of the potential signal of the raw coal sample is consistent with the fluctuation of the load, and the variation of the overall potential signal is relatively stable. The potential signal value of the sandstone sample increases rapidly in the compaction stage and the elastic deformation stage. The potential signal of the granite sample fluctuates greatly in the crack damage and unstable crack propagation stages, and the potential signal value increases faster. By scanning electron microscope and X-ray fluorescence spectrometer, the generation mechanism of potential signals of coal samples with different lithology is explained from the aspects of microstructure and components. The results show that there are more mylonic scratches from the microscopic point of view in the graphite and raw coal samples in the compaction and elastic deformation stages of coal rock loading, which indicates that the friction effect is the important reason for the electrification of the graphite and raw coal samples. The sandstone and granite samples contain more O and Si elements, and the piezoelectric effect is the key reason for the electrification of the sandstone and granite samples, and the potential signal of the sandstone sample is more affected by the piezoelectric effect. In the crack initiation and stable crack growth stage, crack damage and unstable crack propagation and unloading stage, the potential signal generation of each coal sample is mainly caused by the crack propagation and friction effect inside the sample. Among them, crack propagation is an important reason for the generation of coal-rock potential signals. The charge separation at the crack tip mainly includes three aspects, including electron escape caused by stress concentration at the crack tip, crack surface charge separation caused by crack propagation and crack tip discharge.

As the traditional 4th Generation Mobile Communication (4G) bearer network can only meet the 2G, 3G and 4G wireless service backhaul bearer, it cannot meet the higher requirements of 5G wireless backhaul on the bearer network in terms of bandwidth, delay, slicing, and reliability. It also cannot satisfy the extremely demanding real-time protection switching requirements in various 5G service scenarios, as well as the service protection and fast convergence requirements in the enhanced Mobile Broadband (eMBB) scenario. In order to solve this issue, the article proposes a Segmented Routing (SR) tunnel technology in the 5G bearer network protection application scheme. This scheme adopts the connection-oriented SR-Transport Profile (SR-TP) tunnel enhancement technology for the end-to-end protection application of the north-south services in the 5G bearer network, and the optimal SR-Best Effort (SR-BE) tunnel technology for the east-west direction protection applications for connectionless services. Experimental results show that, compared with the traditional 4G bearer network protection scheme, it can fully solve the various requirements of the 5G eMBB service scenario for the bearer network. The service protection switching is completed within 50 ms, which can fully meet the carrier-level protection switching requirements.

Pneumocystis jirovecii is an opportunistic fungus that can cause severe and potentially fatal Pneumocystis pneumonia (PCP) in immunodeficient patients. In this study, we investigated the genetic polymorphisms of P. jirovecii at eight different loci, including six nuclear genes (ITS, 26S rRNA, sod, dhps, dhfr and β-Tub) and two mitochondrial genes (mtLSU-rRNA and cyb) in three PCP cases, including two patients with HIV infection and one without HIV infection in Shanxi Province, P.R. China. The gene targets were amplified by PCR followed by sequencing of plasmid clones. The HIV-negative patient showed a coinfection with two genotypes of P. jirovecii at six of the eight loci sequenced. Of the two HIV-positive patients, one showed a coinfection with two genotypes of P. jirovecii at the same two of the six loci as in the HIV-negative patient, while the other showed a single infection at all eight loci sequenced. None of the three drug target genes (dhfr, dhps and cyb) showed mutations known to be potentially associated with drug resistance. This is the first report of genetic polymorphisms of P. jirovecii in PCP patients in Shanxi Province, China. Our findings expand our understanding of the genetic diversity of P. jirovecii in China.

Due to the uneven distribution of flow velocity when the water flows in the middle section of the bidirectional flow guide device, affect the power generation efficiency of the horizontal axis turbine. By studying the blade radius and installation position of the turbine inside the guide device, improve the power generation efficiency of the turbine inside the two-way guide device in the two-way water flow. First, using Fluent to obtain the flow velocity distribution curve of the vertical section of the center of the diversion device. By means of numerical analysis, established the mathematical model of velocity distribution, and calculated the optimal blade radius. Then, by studying the variation law of flow velocity distribution in the vertical direction, obtained the optimal blade installation position. Finally, through the drag experiment, obtained the periodic power generation under the bidirectional water flow. The research results show that when the blade radius is within the linear range of the flow velocity distribution, the flow velocity of the rotating surface of the turbine blade is larger, and the flow velocity of the rotating surface of the turbine blade increases significantly as the blade installation position decreases. When the water flow velocity is 1 m/s, after adjusting the blade radius and installation position of the turbine, the power generation of the turbine is increased by 72% compared with the previous one, and the power generation by the bare blade is increased by 150%.

ObjectiveScreening bio-samples from a food poisoning event based on ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF MS), and to provide strategies and references for patient treatment as well as the event response.MethodsVomits, gastric lavage and food samples were extracted by acidic acetonitrile and purified by C18 sorbent and analyzed using UPLC-QTOF MS under data independent acquisition mode. Peak identification and spectrum library retrieval were carried out by the Unifi software.ResultsHyoscyamine was detected in 5 human samples and the concentration was below 1 mg/kg. Azithromycin was also screened in one gastric lavage sample. No metabolites of hyoscyamine was found, which was consistent with the result of rat exposure experiment.ConclusionAccording to the screened result, it was inferred that this event was a family food poisoning caused by hyoscyamine. The UPLC-QTOF MS screening is of great significance in dealing with food poisoning emergencies.

The variations in the reference evapotranspiration (ET0) are closely related to meteorological factors. The purpose of this study is to explore the relationships between the meteorological factors and the ET0. Based on meteorological data from 26 meteorological stations in the Yiluo River Basin (YLRB) and its surrounding areas from 1958 to 2020, in this study, the temporal and spatial variations and driving factors of the ET0 in the YLRB are investigated. The results are as follows. Spatially, the annual ET0 decreases from the northeast to the southwest in the YLRB. Temporally, the annual ET0 exhibits a fluctuating decreasing trend rather than a monotonic decreasing trend during the entire period. The trend of the ET0 contains two mutation points, in 1972 and 1994. Thus, the research period can be divided into three periods. It is concluded that the variations in the ET0 are the most sensitive to the relative humidity, but the driving factor that contributes the most to the variations in the ET0 is the wind speed. The driving factors are closely related to the rates of relative change of the meteorological factors.