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Emotion is the external way to express human’s inner thoughts, which has a significant influence on human behaviors. It is an important prerequisite for studying the intrinsic affect mechanism of emotions on behaviors certain. In this article, drivers’ emotional induction experiment, actual and virtual driving experiments are designed to obtain the multi-source dynamic data of human–vehicle–environment under the condition of different emotions. The influences of emotions’ changes on car movement characteristics of different types of drivers are explored. Changing law of car movement characteristics under the condition of different emotions can be obtained finally. The research can provide theoretical basis for the future research of driver assistance system, which is of great significance to realize active vehicle safety warning and unmanned driving in the future.

Most quantum error-correcting codes constructed are stabilizer codes which are potentially more efficient and significant.Under the concept of graph states theory, we focused on 7-qubit graph states characteristics of stabilizer codes, by calculating and analyzing the entanglement properties of graphical codes. For the instance of code ((7,1,3)) with 16 inequitable graphs, the figure of entanglement properties can be measured.And we analyzed 7-qubit graph characteristics of stabilizers codes and calculated the entanglement measures by iterative algorithm.

With the fast development of clean energy in China, the conflict between limited peak load regulation capability and the increasing installed wind power capacity became more and more obvious. Two different wind power integration dispatching modes were proposed in this paper, meanwhile, the wind power integration capacity and system economy under different scenarios, which have different wind power penetration and energy storage system (ESS) capacity, were also analyzed. As indicated in the example analysis, the ESS can significantly enhance the wind power integration capacity; and the economical wind power integration dispatching mode is the most suitable choice for the power grid in wind-rich area.

Indefinite kernel support vector machine (IKSVM) has recently attracted increasing attentions in machine learning. Different from traditional SVMs, IKSVM essentially is a non-convex optimization problem. Some algorithms directly change the spectrum of the indefinite kernel matrix at the cost of losing some valuable information involved in the kernels so as to transform the non-convex problem into a convex one. Other algorithms aim to solve the dual form of IKSVM, but suffer from the dual gap between the primal and dual problems in the case of indefinite kernels. In this paper, we directly focus on the non-convex primal form of IKSVM and propose a novel algorithm termed as IKSVM-DC. According to the characteristics of the spectrum for the indefinite kernel matrix, IKSVM-DC decomposes the objective function into the subtraction of two convex functions and thus reformulates the primal problem as a difference of convex functions (DC) programming which can be optimized by the DC algorithm (DCA). In order to accelerate convergence rate, IKSVM-DC further combines the classical DCA with a line search step along the descent direction at each iteration. A theoretical analysis is then presented to validate that IKSVM-DC can converge to a local minimum. Systematical experiments on real-world datasets demonstrate the superiority of IKSVM-DC compared to state-of-the-art IKSVM related algorithms.

Three novel rare earth complexes [N(CH3)4][Ln(NF)4]6H2O(Ln=Nd(III)(1), Sm(III)(2), Ho(III)(3)) were synthesized using hydrothermal method from norfloxacin HNF=1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3-quinoline carboxylic acid, C16H18FN3O3), imidazole and rare earth nitrates. The complexes were characterized by elemental analysis, FT-IR, TG-DTG and X-ray single crystal diffraction. Each rare earth ion was eight-coordinated with carboxyl-O atoms and keto-O atoms from norfloxacin. Four of the norfloxacin ions acted as bidentate chelate group took part in the coordination with rare earth ion. The structures of complexes were tetragonal system with space group I41/acd, which were allomerism. The interaction between complex 1 and DNA was studied by electronic absorption spectra and fluorescence spectroscopy. The binding interaction between the complex 1 and bovine serum albumin (BSA) was studied by fluorescence spectroscopy. The complex 1 bound to DNA by the mode of partial intercalation. Complex 1 had a strong ability to quench the fluorescence from BSA. The complex interaction was mainly a static quenching process with BSA together with formation of two binding sites.

This paper designs a type of small artificial blood vessel in a composite structure, which the nano cellulose coating is attached to the tube blank. The properties of this type of artificial blood vessel - radical and axial tensile property, area of aperture gaps - are observed by an electron microscopy and analyzed to prepare for the further experiments.

Three novel norcantharidin acylamide acids (L1N-thiadiazole norcantharidin acylamide acid, C10H11N3O4S; L2N-thiazole norcantharidin acylamide acid, C11H12N2O4S and L3N-benzothiazole norcantharidin acylamide acid, C15H14N2O4S) were synthesized by the reactions of norcantharidin (NCTD7-oxabicyclo[2,2,1]heptane-2,3-dicarboxylic acid anhydride, C8H8O4) with 2-amino-1,3,4-thiadiazole (C2H3N3S), 2-aminothiazole (C3H4N2S) and 2-aminobenzothiazole (C7H6N2S), respectively. Their structures were characterized by elemental analysis, IR, and NMR. The inhibition rates of L3 was much higher than those of L1 and L2 against human hepatoma cells SMMC7721 cell lines in vitro. The interaction between the compounds and DNA was studied by means of fluorescence quenching studies and viscosity measurements. The emission intensities decreased obviously with increasing concentration of the compounds in the fluorescence quenching experiments. The linear Stern-Volmer quenching constant Ksq values were 0.62 (L1), 0.55 (L2) and 1.08 (L3), respectively. The binding abilities followed the trend from high to low were L3, L1 and L2, respectively. The results of viscosity measurements showed that L1 and L2 might bind to DNA via partial intercalation, while L3 bound mainly in intercalation.

In a previous study, we reported water-soluble light-emitting nanoparticles with distinct interchain aggregation states of the constituent conjugated polymers. These interchain states usually result in strong self-quenching, dramatically reducing the quantum efficiency of fluorescence. In the work reported in the present study, we developed new water-soluble fluorescent nanoparticles without distinct aggregation of the conjugated polymer chains, which demonstrated distinctive morphologies and optical properties. ‘Strawberry’ morphologies of the nanoparticles were directly observed using transmission electron microscopy. The conjugated polymers were dispersed in the individual cores of the nanoparticles and the majority of the core diameters were in the range 8–12 nm. The primary optical properties of the conjugated polymers in tetrahydrofuran still remained in the nanoparticles. The results suggest that the conjugated polymer chains formed a possible unimolecular structure without distinct aggregation in the nanoparticles. Copyright © 2010 Society of Chemical Industry

Here we report a case of cervical spondylosis misdiagnosed as cerebral infarction. The patient was a 55-year-old man with a one-day history of weakness in his right extremities. Brain magnetic resonance imaging (MRI) showed no acute abnormality, cerevical MRI showed that cervical spondylisis, C4/5, C5/6 disc herniation, spinal canal stenosis and compression of the spinal cord. Then the patient was transferred to the Department of Orthopaedics and underwent surgical treatment of cervical spondylosis. Followed-up for six months, the weakness of his right extremities returned to normal.

The caustic method is applied to compressive shear experiment and used to detect the stress intensity factors of cracks prefabricated on plexiglass sample. Loading, friction of crack planes and influence among cracks are not needed to know as they are combined and transformed into the caustic shadow used in detecting the stress intensity factor. Even boundary condition is not necessary. Therefore it is effective to determine the stress intensity factor of compressive shear crack.

Based on the phenomena that the deformation gap was observed before the great Tangshang earthquake, this paper discusses the strain gap according to test and theory. The (strain) patterns were recorded photographically by realtime holographic interferometry and shadow optical method of caustics, as soon as the loading process started. In the meantime, the AE (acoustic emission) signals were recorded by a micro crack information storage-analysis system. According to damage theory and location of micro fracture, we have studied the stain gap and gained: a) It is necessary that strain gap appears under the condition of linear elasticity theory, and its situation is relatively stable, corresponding to stress concentration. b) Micro fractures, which appear initially at area of high stress, occur rarely at the strain gap, and their locations are finally in the zone between the stress concentration area and the strain gap, which indicate the clusters or groups. However, the major macro fracture (final rupture) started from the shadow areas, and then grew quickly towards the strain gaps, which resulted in failure of sample.

Using a plexiglass sample and by means of real-time holographic interferometry and shadow optical method of caustics, the different features of dynamic variation in stress (strain) field, plastic area and nucleation zone (shadow area) when the sample fractures during loading (loading-fracture) and unloading (unloading-fracture) are studied visually. The results show that the strain nuclei (zones with dense fringes) appear first at the tips of prefabricated cracks at low stress, and then the shadow areas of caustics form with the increase of load. These nuclei and shadow areas can become larger, or smaller, when the process of loading, or unloading, goes on. When the stress is kept within a certain range, the shadow areas of caustics can become larger and smaller alternatively with repeated loading and unloading (cyclic loading). However, when loading and unloading at high stress, in particular when the macrofracture is about to appear, the variations of the shadow areas of caustics are irreversible and quite different. The shadow areas of caustics expand rapidly at an increasing speed when loading-fracture appears. In contrast, the shadow areas of caustics expand at a lower speed when unloading-fracture appears; besides, there is a circular shadow in front of the sharp-angle shaped area.

Rock experiment results indicate that the load/unload response ratio (LURR) of rocks expressed via strain energy may have singular or negative value after the stress in the rock reaches its maximum before rock failure or when the rock goes into the strain-weakening phase. The universality of this phenomenon is discussed. Expressed via strain or strain energy and the travel time of P wave, the variation form of the reciprocal of LURR during the process of rock failure preparation is derived. The results show that after a sharp decrease the reciprocal of LURR reaches its minimum when the main fracture of the rock is about to appear. This feature can be taken as an indication that the rock main fracture is impending.

The purposes of the present study were to investigate the inhibitory effect of quercetin (QUE) preconditioning on endoplasmic reticulum stress (ERS) inducer tunicamycin (TM)-induced apoptosis in RAW264.7 macrophages and the underlying molecular mechanisms. RAW264.7 cells were pretreated with different concentrations (20, 40, and 80 μmol/L) of QUE for 30 min and then treated with TM (5 mg/L) for 12 h. Cell viability and apoptosis were determined using MTT assay and Annexin V-FITC apoptosis detection kit, respectively. The nuclear translocation of activating transcription factor 6 (ATF6) in cells was detected by immunofluorescence analysis and Western blot. Protein and mRNA expressions of C/EBP homologous protein (CHOP) and Bcl-2 were examined by Western blot and real-time PCR, respectively. The results showed that TM reduced cell viability and induced apoptosis in RAW264.7 macrophages. The cytotoxic effects of TM were significantly inhibited by QUE pretreatment at the concentrations of 40 and 80 μmol/L. Interestingly, we found that QUE also significantly suppressed the TM-induced translocation of ATF6, an ERS sensor, from the cytoplasm to the nucleus. In addition, exposure of RAW264.7 macrophages to TM resulted in a significant increase of the expression of CHOP, a transcription factor regulated by ATF6 under conditions of ERS, as well as a decrease of Bcl-2 at transcript and protein levels. QUE blocked these effects in a dose-dependent manner. These data indicate that QUE can protect RAW264.7 cells from TM-induced apoptosis and that the mechanism at least partially involves its ability to inhibit the ATF6-CHOP signaling pathway.

Noroviruses (NoVs) are a leading cause of epidemic acute gastroenteritis affecting millions of people worldwide. Understanding of NoV remains limited due to the lack of a cell culture system and small animal models. Currently, there are no available vaccines or antivirals against NoVs. In this study, an approach for large-scale production of anti-NoV antibodies for use as a potential treatment for NoV disease using passive immunization was evaluated. NoV-specific immunoglobulins (IgY) were produced by immunizing chickens with NoV P particles. The birds continuously produced high titers of antibodies in their eggs for at least 3 months, in which NoV-specific antibody levels reached 4.7-9.2 mg/egg yolk. The egg yolk antibodies strongly reacted with NoV P particles by both ELISA and Western blot and blocked NoV virus-like particle (VLP) and P particle binding to the histo-blood group antigen (HBGA) receptors with a BT50 of about 1:800. The blocking activity of the chicken IgY remained after an incubation at 70°C for 30 min or treatment at pH 4 to 9 for 3 h. These data suggested that chicken IgY could be a practical strategy for large-scale production of anti-NoV antibodies for potential use as passive immunization against NoV infection, as well as for diagnostic purposes.

Among the new norcantharidin acylamide acids synthesized, compound (VII) shows the highest antiproliferative activities against human hepatoma cells SMMC7721 in vitro.

Two new La(III) and Sm(III) complexes with enrofloxacin (HER, 1-cyclopropyl-7-(4-ethyl-1-piperazinyl)-6-fluoro-1,4-dihydro-4-oxo-3-quinoline carboxylic acid, C19H21FN3O3), [La2(ER)6(H2O)2]·14H2O(1) and [Sm2(ER)6(H2O)2]·14H2O(2) have been synthesized and characterized by elemental analysis, FT-IR, TG-DTG and X-ray single crystal diffraction. Both of the complexes are triclinic system with space group Pī. The structure of the complexes show that each rare earth atom in both complexes was nine-coordinated. Two of the enrofloxacin ions acted as tridentate chelate and bridging ligands, while the others as bidentate chelate ligands. The binding reaction between the complexes and bovine serum albumin (BSA) was studied by UV-vis absorption spectra and fluorescence spectroscopy. The results indicated that the two complexes had a quite strong ability to quench the fluorescence from BSA and the binding reaction was mainly a static quenching process. The binding constants K A /(L·mol−1) were 1.46 × 105(1) and 8.59 × 106(2) and one binding site was formed. The synchronous spectroscopy suggested that tryptophan residues were placed in BSA. It was also found that the two complexes exhibited greater antimicrobial activity than enrofloxacin at given concentrations.

Three novel complexes [Nd(L)(NO3)(H2O)2].NO(3).2H2O (HL1 = N-pyrimidine norcantharidin acylamide acid, C12H13N3O4; HL2 = N-pyridine norcantharidin acylamide acid, C13H14N2O4; HL3 = N-phenyl norcantharidin acylamide acid, C14H15NO4) were synthesized. HL1, HL2 and HL3 are the ligand of complex(1), complex(2) and complex(3), respectively. Their structures were characterized by elemental analysis, conductivity measurement, infrared spectra and thermogravimetric analysis. The DNA-binding properties of the complexes have been investigated by fluorescence spectroscopy and viscosity measurements. The results suggest that the complexes can bind to DNA by partial intercalation. The liner Stern-Volmer quenching constant Ksq values are 3.3(+/-0.21)(1), 1.7(+/-0.19)(2) and 0.9(+/-0.04)(3), respectively. Complex (1) and (2) have been found to cleave pBR322 plasmid DNA at physiological pH and temperature. The test of antiproliferation activity indicates that complex(1) has strong antiproliferative ability against the SMMC7721 (IC50 = 131.7 +/- 23.4 micromol x L(-1)) and A549 (IC50 = 128.4 +/- 19.9 micromol x L(-1)) cell lines. The inhibition rates of complex(2) (IC50 = 86.3 +/- 11.3 micromol x L(-1)) are much higher than that of NCTD (IC50 = 115.5 +/- 9.5 micromol x L(-1)) and HL2 (111.0 +/- 5.7 micromol x L(-1)) against SMMC7721 cell lines.

On a plexiglass sample, a penetrating crack was prefabricated by laser. The crack is inclined towards the major principal stress (σ1 (σ y ,) at an angle of about 30o. Using this sample and by means of shadow optical method of caustics and microcrack location, the process zone, nucleation zone and plastic area of earthquakes were studied experimentally, and the strain variation in the shadow area was monitored. From the result, we comprehend the following. When the stress σ y , was increased to a certain value, shadow areas were formed around the prefabricated crack and at its tips, with that at the tips being larger. These shadow areas become larger with the increase of load and smaller with unloading. In the shadow area the strain was inhomogeneous: it was very large in some places but very small in others. When the shadow area reached a critical state, microcracks appeared at the tips of the prefabricated crack. Microcracks appeared on one side of the prefabricated crack where the strain and the shadow were both smaller. The zone with concentrated microcracks, or the process zone, was always located at the crack tip; this zone together with a half length of the original crack formed the nucleation zone which fell into the shadow area but was smaller than it. The shadow optical area of caustics bears a certain quantitative relation with the plastic area. Therefore, if an appropriate method is available to obtain the shadow optical area of caustics, it would be possible to detect the area with strong differential deformation change, and hence to determine the zone where strong fracture (an earthquake) would take place.

Novel noncovalently connected water-soluble nanoparticles containing hydroxyl-capped polyfluorene (PFOH) and poly(acrylic acid) (PAA) were obtained and characterized. These nanoparticles were quite...

In the crystal structure of the title compound, [Ni(C(8)H(8)O(5))(H(2)O)(2)](n), the Ni(II) cation is in a Jahn-Teller-distorted octahedral coordination environment binding to two O atoms from water molecules, the bridging O atom of the bicycloheptane unit, two carboxylate O atoms from different carboxylate groups and one carboxylate O atom from a symmetry-related bridging ligand. The crystal structure is made up from layers propagating parallel to the bc plane.