128 results on '"Yumiko Hori"'
Search Results
52. Angiogenic Factor with G-patch and FHA Domain 1 (AGGF1) Expression in Human Vascular Lesions
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Maosheng Zhan, Jun-ichiro Ikeda, Yumiko Hori, Eiichi Morii, Yuuki Hata, Keigo Osuga, and Naoki Wada
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AGGF1 ,Pathology ,medicine.medical_specialty ,Histology ,Capillary malformation ,Physiology ,Angiogenesis ,Biochemistry ,vascular malformation ,Pathology and Forensic Medicine ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,In vivo ,Medicine ,business.industry ,Vascular malformation ,Regular Article ,Cell Biology ,Mast cell ,medicine.disease ,Vascular endothelial growth factor B ,Vascular endothelial growth factor ,medicine.anatomical_structure ,Vascular endothelial growth factor C ,chemistry ,030220 oncology & carcinogenesis ,vascular tumor ,mast cell ,business - Abstract
Angiogenic factor with G-patch and FHA domain 1 (AGGF1) is a novel angiogenic factor that was first described in Klippel-Trenaunay syndrome, a congenital vascular disease associated with capillary and venous malformations. AGGF1, similar to vascular endothelial growth factor (VEGF), has been shown to promote strong angiogenesis in chick embryos in vivo. Blocking AGGF1 expression prevented vessel formation, which suggests AGGF1 is a potent angiogenic factor linked to vascular malformations. So far, AGGF1 expression studies in human vascular lesions have not been performed. Here, we immunohistochemically investigated AGGF1 expression in venous, arteriovenous or capillary malformations, and infantile or congenital hemangioma. We found that AGGF1 was mostly expressed in endothelial cells with plump morphology. Moreover, the majority of mast cells strongly expressed AGGF1. Notwithstanding our incomplete knowledge of the molecular mechanism of AGGF1 in angiogenesis, our results show for the first time that AGGF1 is expressed in plump endothelial cells and mast cells.
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- 2016
53. Recurrent Stroke Due to Metastatic Pulmonary Tumor Emboli as an Important Clinical Entity
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Kenji Ohshima, Yasukazu Terasaki, Manabu Sakaguchi, Yasufumi Gon, Susumu Nakahara, Junji Takasugi, Hideki Mochizuki, Eiichi Morii, Yumiko Hori, Tsutomu Sasaki, and Naoki Oyama
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Male ,medicine.medical_specialty ,Lung Neoplasms ,Biopsy ,Autopsy ,Fibrin Fibrinogen Degradation Products ,03 medical and health sciences ,Fatal Outcome ,0302 clinical medicine ,Recurrence ,medicine ,Humans ,Stroke ,Aged ,Neoplasm Staging ,Rivaroxaban ,Squamous Cell Carcinoma of Head and Neck ,business.industry ,Tumor Embolism ,Rehabilitation ,Anticoagulants ,Heparin ,Neoplastic Cells, Circulating ,medicine.disease ,Surgery ,Oropharyngeal Neoplasms ,Venous thrombosis ,Diffusion Magnetic Resonance Imaging ,Treatment Outcome ,Intracranial Embolism ,Respiratory failure ,Head and Neck Neoplasms ,Lymphatic Metastasis ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Neurology (clinical) ,Radiology ,Respiratory Insufficiency ,Cardiology and Cardiovascular Medicine ,business ,Biomarkers ,030217 neurology & neurosurgery ,Chemoradiotherapy ,medicine.drug - Abstract
We present an autopsy case of repetitive stroke due to tumor emboli, indistinguishable from thromboembolism with a hypercoagulable state in its clinical course. A 72-year-old man diagnosed with stage IVA oropharyngeal squamous cell carcinoma received chemoradiotherapy. Follow-up imaging revealed mediastinal lymph nodes and pulmonary metastasis. One year later, the patient experienced right arm weakness, and brain magnetic resonance imaging showed acute ischemic lesions in multiple vascular territories. He was diagnosed with paradoxical cerebral embolism due to cancer-associated venous thrombosis and treated with rivaroxaban. However, newly developed cerebral infarcts were confirmed 1 month later. Then, rivaroxaban treatment was switched to subcutaneous unfractionated heparin injection. He was admitted again for stroke recurrence and died of respiratory failure 8 days after admission. Autopsy demonstrated pulmonary metastasis invading the veins and tumor emboli in the culprit cerebral arteries. D-dimer was kept constant at a slightly higher level, ranging from 1 to 3 µg/mL during the course of recurrence. We should consider tumor embolism in the differential diagnosis of recurrent stroke along with pulmonary tumor and resistance to heparin preparations with unchanged D-dimer levels.
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- 2017
54. Origin of cancer-associated fibroblasts and tumor-associated macrophages in humans after sex-mismatched bone marrow transplantation
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Masaharu Kohara, Shinichiro Tahara, Satoshi Nojima, Eiichi Morii, Koichi Miyamura, Yumiko Hori, Masako Kurashige, Naoki Wada, Jun-ichiro Ikeda, Masafumi Ito, and Kenji Ohshima
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0301 basic medicine ,Bone marrow transplantation ,Disease progression ,Mammary gland ,Medicine (miscellaneous) ,In situ hybridization ,Biology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,lcsh:Biology (General) ,030220 oncology & carcinogenesis ,Cancer research ,medicine ,Cancer-Associated Fibroblasts ,Bone marrow ,Oral mucosa ,General Agricultural and Biological Sciences ,lcsh:QH301-705.5 ,Immunostaining - Abstract
Cancer-associated fibroblasts (CAFs) and tumor-associated macrophages (TAMs) in tumor stroma play a key role in disease progression. Recent studies using mice models suggest that CAFs are partly derived from bone marrow and TAMs primarily originate from bone marrow-derived inflammatory monocytes. However, the origin of these cells in humans remains unclear. Hence, we investigated their human origin, using specimens from human secondary tumors that developed after sex-mismatched bone marrow transplantation, by modified immunofluorescent in situ hybridization analysis and triple immunostaining. We observed that most of the α-smooth muscle actin (αSMA)-positive CAFs in the mammary gland, liver, and oral mucosa specimens obtained 3–19 years after bone marrow transplantation are recipient-derived cells. In contrast, the majority of the peritumoral αSMA-negative fibroblast-like cells are actually bone marrow-derived HLA-DR-positive myeloid cells, such as macrophages and dendritic cells. Furthermore, almost all CD163-positive TAMs and macrophages present in the non-tumor areas are derived from bone marrow.
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- 2018
55. Persistent methicillin-resistant Staphylococcus aureus bacteremia owing to placental abscess
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Tetsuo Maeda, Hideharu Hagiya, Yumiko Hori, Keigo Kimura, Yuzuru Kanakura, Kazunori Tomono, Tadashi Kimura, Shinsuke Kusakabe, Keisuke Kawasaki, Eiichi Morii, Tsuyoshi Takiuchi, Sayuri Iwai, and Naomi Maeda
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0301 basic medicine ,Microbiology (medical) ,Adult ,Methicillin-Resistant Staphylococcus aureus ,medicine.medical_specialty ,Abdominal Abscess ,Pancytopenia ,Placenta ,030106 microbiology ,Bacterial Toxins ,Exotoxins ,Bacteremia ,Tretinoin ,medicine.disease_cause ,Staphylococcal infections ,Chorioamnionitis ,03 medical and health sciences ,0302 clinical medicine ,Leukemia, Promyelocytic, Acute ,Leukocidins ,Pregnancy ,Internal medicine ,Medicine ,Humans ,Pharmacology (medical) ,030212 general & internal medicine ,Abscess ,business.industry ,SCCmec ,Toxic shock syndrome ,biochemical phenomena, metabolism, and nutrition ,Staphylococcal Infections ,bacterial infections and mycoses ,medicine.disease ,Methicillin-resistant Staphylococcus aureus ,Infectious Diseases ,Female ,business ,Febrile neutropenia - Abstract
Staphylococcus aureus as a pathogen in human gestational membranes, a rather rare phenomenon, has recently been the focus of several researches. S. aureus forms biofilms on these membranes and potentially causes chorioamnionitis in pregnant women. We report a case of persistent methicillin-resistant S. aureus (MRSA) bacteremia owing to placental infection, causing chorioamnionitis and preterm birth. A 29-year-old Japanese woman at the 27th gestational week was diagnosed with acute promyelocytic leukemia and underwent all-trans retinoic acid therapy. Soon after hospitalization, the patient presented with persistent MRSA bacteremia of unknown origin. Despite various antimicrobial therapies, she experienced 12 MRSA bacteremia episodes over 6 weeks. However, after child birth, MRSA bacteremia disappeared without any complications. A pathologic examination of her placenta revealed placenta abscess, resulting in a diagnosis of MRSA-associated chorioamnionitis. Molecular analysis proved that a single MRSA strain (SCCmec Type IVa), which tested negative for Panton-Valentine leukocidin and toxic shock syndrome toxin-1, caused the obstinate infection. We should be aware that persistent MRSA bacteremia in pregnant women can originate from placental abscess.
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- 2018
56. Poorly differentiated hepatocellular carcinoma accompanied by anti-Hu antibody-positive paraneoplastic peripheral neuropathy
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Shigeru Marubashi, Eiichi Morii, Naoki Wada, Michiie Sakamoto, Yumiko Hori, Hiroshi Wada, Jun-ichiro Ikeda, Hidetoshi Eguchi, Hiroaki Nagano, and Takahiro Matsui
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Pathology ,medicine.medical_specialty ,Liver tumor ,biology ,business.industry ,Chromogranin A ,Vimentin ,General Medicine ,medicine.disease ,Pathology and Forensic Medicine ,Lesion ,Cytokeratin ,Poorly Differentiated Hepatocellular Carcinoma ,biology.protein ,Synaptophysin ,Medicine ,Small Cell Lung Carcinoma ,medicine.symptom ,business - Abstract
The anti-Hu antibody is one of the most famous onco-neural antibodies related to paraneoplastic neurological syndrome, and is associated with small cell lung carcinoma in most cases. Here, we report a case of poorly differentiated hepatocellular carcinoma accompanied by paraneoplastic peripheral neuropathy positive for the anti-Hu antibody. Image inspection before operation revealed that no tumors were found in organs other than the liver, including lung, and that the liver tumor had no metastatic lesion. The liver tumor showed histological appearance of poorly differentiated carcinoma with cartilaginous metaplasia and partial blastoid cell appearance. Most tumor cells presented trabecular-like structure lined by sinusoidal vessels. Immunohistochemically, the tumor cells were positive for low molecular weight cytokeratin and vimentin, partially positive for cytokeratin 19 and CD56, but negative for synaptophysin, chromogranin A and alpha-fetoprotein. Based on the trabecular-like morphology and the results of immunohistochemical staining, we concluded that the tumor was diagnosed as poorly differentiated hepatocellular carcinoma. Anti-Hu antibody-positive paraneoplastic peripheral neuropathy accompanied with liver tumor is extremely rare as far as is known. The presented case indicates that poorly differentiated carcinoma has the potential to be the responsible lesion of anti-Hu antibody-positive paraneoplastic neurological syndrome and systemic work-up is important for the management of this neurological disorder.
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- 2015
57. Present status of steroid administration to patients with autoimmune hepatitis and factors associated with relapse
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Yoshinori Doi, Takayuki Yakushijin, Hiroyuki Fukui, Naoki Hiramatsu, Tomohide Tatsumi, Eijiro Hayashi, Eiji Mita, Fumihiko Nakanishi, Narihiro Shibukawa, Shusaku Tsutsui, Yasuharu Imai, Masahide Oshita, Nobuyuki Tatsumi, Harumasa Yoshihara, Eiichi Morii, Tetsuo Takehara, Ichizo Kobayashi, Akira Kaneko, and Yumiko Hori
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Hepatology ,business.industry ,medicine.medical_treatment ,Immunology ,medicine ,Autoimmune hepatitis ,medicine.disease ,business ,Administration (government) ,Steroid - Published
- 2015
58. Adenylosuccinate lyase enhances aggressiveness of endometrial cancer by increasing killer cell lectin-like receptor C3 expression by fumarate
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Shinichiro Tahara, Satoshi Nojima, Jun-ichiro Ikeda, Daisuke Okuzaki, Eiichi Morii, Naoki Wada, Yumiko Hori, Masako Kurashige, Haengki Park, and Kenji Ohshima
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0301 basic medicine ,Cell ,Population ,Biology ,Adenocarcinoma ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Fumarates ,Cell Movement ,Cell Line, Tumor ,medicine ,Humans ,Neoplasm Invasiveness ,Adenylosuccinate lyase ,Receptor ,education ,Molecular Biology ,Cell Proliferation ,Gene knockdown ,education.field_of_study ,Cell growth ,Adenylosuccinate Lyase ,Cancer ,Cell Biology ,medicine.disease ,Endometrial Neoplasms ,030104 developmental biology ,medicine.anatomical_structure ,Cell culture ,030220 oncology & carcinogenesis ,Cancer research ,Female ,NK Cell Lectin-Like Receptor Subfamily C - Abstract
Adenylosuccinate lyase (ADSL) is an enzyme that plays important roles in de novo purine synthesis. Although ADSL was reported to be upregulated in various malignancies, such as colorectal, breast, and prostate cancer, as well as gliomas, the mechanism by which elevated ADSL expression contributes to cancer has not been elucidated. We previously performed a shotgun proteomics analysis to characterize specific proteins associated with the properties of the aldehyde dehydrogenase (ALDH)-high cell population, which was reported to be involved in tumorigenic potential, and showed that ADSL expression is upregulated in the ALDH-high population of endometrial cancer. Here, we showed that ADSL is involved in endometrial cancer aggressiveness by regulating expression of killer cell lectin-like receptor C3 (KLRC3), which is a receptor expressed on natural killer cells. Immunohistochemical analysis indicated that ADSL expression increased as endometrioid carcinoma specimens became more poorly differentiated and higher degree of primary tumor progression. Knockdown of ADSL in endometrial cancer cells decreased cell proliferation, migration, and invasive capability, and caused the cells to adopt a more rounded shape. DNA microarray analysis and quantitative real-time PCR showed that KLRC3 expression was decreased in ADSL knockdown cells. Knockdown of KLRC3 in endometrial cancer cells resulted in the same phenotype as knockdown of ADSL. Moreover, fumarate, which could be produced by ADSL and was recently shown to be an oncometabolite, recovered KLRC3 expression in ADSL knockdown cells, suggesting that fumarate produced by ADSL could regulate KLRC3 expression. Our findings indicate that ADSL enhances cell proliferation, migration, and invasive capability through regulation of KLRC3 expression by fumarate.
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- 2017
59. Argininosuccinate Synthase 1-Deficiency Enhances the Cell Sensitivity to Arginine through Decreased DEPTOR Expression in Endometrial Cancer
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Kohei Hagiwara, Satoshi Nojima, Shinichiro Tahara, Shinya Oki, Masako Kurashige, Yumiko Hori, Eiichi Morii, Yoshikatsu Kanai, Jun-ichiro Ikeda, Kenji Ohshima, Naoki Wada, and Daisuke Okuzaki
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0301 basic medicine ,Arginine ,mTORC1 ,Mechanistic Target of Rapamycin Complex 1 ,DEPTOR ,Article ,03 medical and health sciences ,Cell Movement ,Tumor Cells, Cultured ,Humans ,Mechanistic target of rapamycin ,Cell Proliferation ,Citrullinemia ,Tumor microenvironment ,Multidisciplinary ,biology ,Cell growth ,Chemistry ,Intracellular Signaling Peptides and Proteins ,Endometrial Neoplasms ,Argininosuccinate Synthetase 1 ,030104 developmental biology ,Cancer research ,biology.protein ,Female ,Signal transduction ,Signal Transduction - Abstract
Argininosuccinate synthetase 1 (ASS1) is a rate-limiting enzyme in arginine biosynthesis. Although ASS1 expression levels are often reduced in several tumors and low ASS1 expression can be a poor prognostic factor, the underlying mechanism has not been elucidated. In this study, we reveal a novel association between ASS1 and migration/invasion of endometrial tumors via regulation of mechanistic target of rapamycin complex (mTORC) 1 signaling. ASS1-knockout cells showed enhanced migration and invasion in response to arginine following arginine starvation. In ASS1-knockout cells, DEPTOR, an inhibitor of mTORC1 signal, was downregulated and mTORC1 signaling was more activated in response to arginine. ASS1 epigenetically enhanced DEPTOR expression by altering the histone methylation. Consistent with these findings, tumor cells at the invasive front of endometrioid carcinoma cases showed lower ASS1 and DEPTOR expression. Our findings suggest that ASS1 levels in each tumor cell are associated with invasion capability in response to arginine within the tumor microenvironment through mTORC1 signal regulation.
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- 2017
60. Importance of Early Appropriate Intervention Including Antibiotics and Wound Care for Device-Related Infection in Patients With Left Ventricular Assist Device
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S. Nakajima, Takahiro Sato, Yumiko Hori, T. Watanabe, Masanobu Yanase, Hiroki Hata, Takeshi Nakatani, Osamu Seguchi, Michinari Hieda, Y. Murata, M. Sata, Kyoichi Wada, T. Fujita, J. Kobayashi, and Haruki Sunami
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Adult ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Wound care ,medicine ,Humans ,Antibiotic prophylaxis ,Retrospective Studies ,Transplantation ,Teicoplanin ,business.industry ,Incidence (epidemiology) ,Doripenem ,Bacterial Infections ,Antibiotic Prophylaxis ,equipment and supplies ,medicine.disease ,Cannula ,Anti-Bacterial Agents ,Surgery ,Occlusive dressing ,Carbapenems ,Ventricular assist device ,Heart failure ,Wounds and Injuries ,Female ,Heart-Assist Devices ,business ,medicine.drug - Abstract
Introduction A left ventricular assist device (LVAD) is essential for treating patients with advanced heart failure. However, LVAD-related infection is a significant cause of mortality and morbidity, with bloodstream infection (BSI) especially associated with high mortality. We investigated the incidence of infectious complications in patients who received an LVAD and evaluated the effects of early and appropriate intervention for LVAD-related infection. Method We retrospectively reviewed 27 consecutive patients who underwent continuous-flow LVAD (CF-LVAD; n = 16) or pulsatile-flow LVAD (PF-LVAD; n = 11) implantation at the National Cerebral and Cardiovascular Center between April 2011 and March 2013. Incidences of LVAD-related infections, such as drive-line infection in patients with CF-LVAD, cannula infection in patients with PF-LVAD, and BSI in patients with both types, were examined (follow-up period, 342 ± 229 days). The mandatory antibiotic prophylaxis protocol at our institution includes teicoplanin (400 mg) 2 days before LVAD implantation and doripenem (1000 mg) within 1 hour of skin incision. In addition, the driveline exit sites undergo sterile cleansing with diluted hydrogen peroxide and placement of an antimicrobial occlusive dressing for wound care, with dressing changes performed 2-3 times per day. Results More than 90% of all patients suffered from a drive-line infection within 12 months after LVAD implantation. However, BSI developed in only 12.5% of CF-LVAD and 10% of PF-LVAD patients within 12 months (log-rank test; P = .875). Conclusions LVAD-related infections, such as drive-line and cannula infections, were common, whereas the incidence of BSI was low in our LVAD-implanted patients. Our results highlight the importance of early and appropriate intervention including antibiotics and wound care for device-related infections for reducing the incidence of potentially fatal BSI.
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- 2014
61. Fluorescent colored material made of clay mineral and phycoerythrin pigment derived from seaweed
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Saki Hoshino, Yi-Hung Lin, Yoshiumi Kohno, Masashi Shibata, Chiho Miyazawa, and Yumiko Hori
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chemistry.chemical_classification ,Aqueous solution ,Process Chemistry and Technology ,General Chemical Engineering ,Salt (chemistry) ,Mineralogy ,Fluorescence ,chemistry.chemical_compound ,Pigment ,Montmorillonite ,Adsorption ,chemistry ,Chemical engineering ,visual_art ,Hectorite ,visual_art.visual_art_medium ,sense organs ,Clay minerals - Abstract
In order to obtain a fluorescent colored powder, composites of phycoerythrin pigment extracted from seaweed and clays (hectorite and montmorillonite) were examined. The pigment adsorbed easily on synthetic hectorite in an aqueous solution and a composite with bright fluorescence was obtained. On the other hand, the adsorption of the pigment on natural montmorillonite was not sufficient, and a salt had to be added to the pigment solution to obtain the composite. Low heat and light fastness, which are serious drawbacks of phycoerythrin for practical usage, were increased by the adsorption to the hectorite clay.
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- 2014
62. A community-based cross-sectional and longitudinal study uncovered asymptomatic proteinuria in Japanese adults with low body weight
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Setsuko Seta, Hitoshi Sugawara, Shin-ichi Momomura, Kei Nakajima, Haruki Oshida, Kaname Suwa, Takeshi Ishida, Masafumi Kakei, Toshitaka Muneyuki, Masafumi Saito, and Yumiko Hori
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Adult ,Male ,medicine.medical_specialty ,Longitudinal study ,Time Factors ,Cross-sectional study ,urologic and male genital diseases ,Asymptomatic ,Young Adult ,Asian People ,Japan ,Recurrence ,Risk Factors ,Internal medicine ,Odds Ratio ,Prevalence ,Humans ,Medicine ,Longitudinal Studies ,Aged ,Retrospective Studies ,Chi-Square Distribution ,Proteinuria ,urogenital system ,business.industry ,Incidence ,Incidence (epidemiology) ,Body Weight ,Odds ratio ,Middle Aged ,medicine.disease ,Obesity ,female genital diseases and pregnancy complications ,Cross-Sectional Studies ,Logistic Models ,Endocrinology ,Nephrology ,Asymptomatic Diseases ,Multivariate Analysis ,Linear Models ,Female ,medicine.symptom ,business ,Body mass index - Abstract
Although proteinuria is highly prevalent in obese individuals, the association between proteinuria and low body weight is equivocal. In this study we determine whether low body weight is more strongly associated with proteinuria compared with normal weight. The association between body mass index (BMI) and proteinuria was examined in a cross-sectional study of 62,582 asymptomatic individuals aged 20-70 years without known kidney diseases recruited, based on the results of medical checkups in 1999. We also examined the incidence of recurrent or nonrecurrent proteinuria in an 8-year longitudinal analysis of 12,493 individuals without proteinuria at baseline. The prevalence of proteinuria showed a J-shaped relationship with BMI. Multivariate regression analysis showed that BMI of 27.0 kg/m(2) and above or 18.9 kg/m(2) and less was significantly associated with proteinuria relative to BMI 21.0-22.9 kg/m(2), even after adjusting for relevant cardiometabolic risk factors. In the longitudinal study, similar J-shaped relationships between the incident rates of proteinuria and baseline BMI groups were observed at post-baseline checkups. Baseline BMI 27.0 kg/m(2) and above was associated with significantly greater risk for recurrent and nonrecurrent proteinuria, whereas BMI 18.9 kg/m(2) and less was only associated with nonrecurrent proteinuria. Thus, obesity and low body weight may be associated with different types of proteinuria independent of cardiometabolic risk factors.
- Published
- 2013
63. Expression of FoxO3a in clinical cases of malignant lymphoma
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Tian Tian, Keiichiro Honma, Eiichi Morii, Naoki Wada, Yi Wang, Yumiko Hori, and Jun-ichiro Ikeda
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Pathology ,medicine.medical_specialty ,Follicular lymphoma ,Pathology and Forensic Medicine ,Nodular sclerosis ,immune system diseases ,hemic and lymphatic diseases ,Biomarkers, Tumor ,Humans ,Medicine ,T-cell lymphoma ,Phosphorylation ,Anaplastic large-cell lymphoma ,Cellular Senescence ,business.industry ,Lymphoma, Non-Hodgkin ,Forkhead Box Protein O3 ,Forkhead Transcription Factors ,Cell Biology ,medicine.disease ,Marginal zone ,Hodgkin Disease ,Immunohistochemistry ,Lymphoma ,Leukemia ,Ki-67 Antigen ,Mantle cell lymphoma ,business ,Proto-Oncogene Proteins c-akt - Abstract
Cancer-initiating cells (CICs) are a limited number of cells with tumorigenic activity. Few studies have been performed on CICs in malignant lymphoma. We recently demonstrated that a small number of FoxO3a-expressing cells possessed CIC-like potential in Hodgkin's lymphoma (HL) cell lines. In the present study, FoxO3a expression was examined immunohistochemically in 137 patients with malignant lymphoma. Among patients with HL, FoxO3a-positive tumor cells were detected in 11 of 11 with nodular sclerosis classical HL, 8 of 15 with mixed cellularity classical HL, 0 of 1 with lymphocyte-rich classical HL, and 2 of 3 with nodular lymphocyte-predominant HL. Only limited numbers of patients with non-HL expressed FoxO3a: 4 of 66 with diffuse large B-cell lymphoma, 1 of 20 with follicular lymphoma, and 1 of 5 with peripheral T-cell lymphoma, not otherwise specified. No FoxO3a expression was detected in patients with mantle cell lymphoma (n=3), extranodal marginal zone B-cell lymphoma (n=3), mediastinal large B-cell lymphoma (n=1), NK/T cell lymphoma (n=5), anaplastic large cell lymphoma (n=2), or T-lymphoblastic lymphoma/leukemia (n=2). These results suggest that FoxO3a is expressed mostly in patients with HL, but not in patients with non-HL. FoxO3a expression was limited to a small number of Hodgkin cells in a quiescent state. FoxO3a may be a CIC marker of HL, but not of non-HL.
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- 2013
64. Artificial Sweeteners and Glucose Metabolism: A Review of the Literature Published Since 2000
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Masafumi Saito, Yumiko Hori, and Kei Nakajima
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business.industry ,Medicine ,General Medicine ,Food science ,Carbohydrate metabolism ,business ,Artificial Sweetener - Published
- 2013
65. Design of the Saitama Cardiometabolic Disease and Organ Impairment Study (SCDOIS): A Multidisciplinary Observational Epidemiological Study
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Yumiko Hori, Ayano Kutsuma, Shin-ichi Momomura, Kaname Suwa, Masafumi Saito, Tsukasa Yoshida, Eiichiro Kanda, Kei Nakajima, Yoshihiko Kannno, Masafumi Kakei, Toshitaka Muneyuki, Tomoya Takaoka, and Haruki Oshida
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Pediatrics ,medicine.medical_specialty ,business.industry ,Incidence (epidemiology) ,Type 2 diabetes ,Overweight ,medicine.disease ,Obesity ,Diabetes mellitus ,Epidemiology ,Physical therapy ,Medicine ,Observational study ,medicine.symptom ,business ,Dyslipidemia - Abstract
Background: In the past few decades, the incidence of cardiometabolic diseases and disorders of the liver, kidney, pancreas, heart, and lung, have been increasing in Western and Asian countries, including Japan. Numerous factors, including abnormal body weight (obesity, overweight, or underweight), infrequent exercise, and other unfavorable lifestyle factors (e.g., smoking and heavy alcohol drinking) have been proposed as risk factors for the development and the progression of diseases, such as type 2 diabetes, hypertension, and dyslipidemia, which ultimately lead to impaired organ function and possibly death. However, the mechanisms that link these risk factors with diseases are still poorly understood, and the potential treatments, including pharmacotherapy and diet, have not been fully evaluated. Methods: In 2011, we established a new collaborative research program, the Saitama Cardiometabolic Disease and Organ Impairment Study (SCDOIS). This multidisciplinary observational epidemiological research study was designed to cover several high-profile diseases and some traditional fields of internal medicine, as well as apparently unrelated fields and particular lifestyle factors, such as unhealthy eating behaviors. In a series of studies, apparently healthy subjects who underwent a regular medical checkup were retrospectively identified based on the results of their medical checkups. In this way, the incidence, prevalence, causality, and clinical relevance of specific conditions and diseases have been investigated in cross-sectional analyses of 100,000 - 200,000 adults, and in longitudinal studies of several thousand subjects who underwent medical checkups multiple times. Discussion: This article describes the background, rationale, purpose, and methods of the SCDOIS. Using data obtained from annual medical checkups, our goals are to 1) establish criteria or identify clinical features that would enable clinicians to detect the presence of abnormal conditions associated with cardiometabolic diseases and/or organ impairment much earlier in the disease course; and 2) determine the potential mechanisms and therapies for these conditions.
- Published
- 2013
66. Vulvovaginal aggressive angiomyxoma mimicking lymphedema complicated with Behcet’s disease: a case report and review of the literature
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Tadashi Kimura, Tetsu Wakimoto, Masami Fujita, Yumiko Hori, Yutaka Ueda, Eiichi Morii, Eiji Kobayashi, Toshihiro Kimura, Kiyoshi Yoshino, and Shinya Matsuzaki
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Vulvovaginal Region ,medicine.medical_specialty ,business.industry ,Behcet's disease ,medicine.disease ,Dermatology ,Benign tumor ,Surgery ,Aggressive angiomyxoma ,Lymphedema ,Edema ,medicine ,medicine.symptom ,Angiomyxoma ,business ,Rare disease - Abstract
This report describes a case of aggressive angiomyxoma (AAM), arising in the vulvovaginal region, which was initially misdiagnosed as lymphedema due to a vaginal ulcer caused by Behcet’s disease. AAM is a very rare disease, usually found only in women of reproductive age, occurring predominantly in the pelviperineal region. AAM is basically a benign tumor usually treated by surgical resection. However, even after apparently complete resection, local recurrence of the tumor often occurs, thus its characterization as “aggressive” angiomyxoma. In contrast, vulvar edema is a condition with a very similar appearance that is observed relatively commonly, and which has many causes, e.g., obesity, inflammation, and benign or malignant tumors. Here we report a case of AAM which was initially mistaken for vulvovaginal edema, and initially believed to arise as a result of an ulcerative vaginal lesion associated with the patient’s underlying Behcet’s disease. We have reviewed the literature concerning vulvovaginal edema. We present herein this work to others as a precautionary tale, to prevent similar misdiagnosis and delay of treatment.
- Published
- 2012
67. Project conducted in Hirakata to improve cervical cancer screening rates in 20-year-old Japanese: Influencing parents to recommend that their daughters undergo cervical cancer screening
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Asami, Yagi, Yutaka, Ueda, Tomomi, Egawa-Takata, Yusuke, Tanaka, Yoshito, Terai, Masahide, Ohmichi, Tomoyuki, Ichimura, Toshiyuki, Sumi, Hiromi, Murata, Hidetaka, Okada, Hidekatsu, Nakai, Masaki, Mandai, Shinya, Matsuzaki, Eiji, Kobayashi, Kiyoshi, Yoshino, Tadashi, Kimura, Junko, Saito, Yumiko, Hori, Eiichi, Morii, Tomio, Nakayama, Yukio, Suzuki, Yoko, Motoki, Akiko, Sukegawa, Mikiko, Asai-Sato, Etsuko, Miyagi, Manako, Yamaguchi, Risa, Kudo, Sosuke, Adachi, Masayuki, Sekine, Takayuki, Enomoto, Yorihiko, Horikoshi, Tetsu, Takagi, and Kentaro, Shimura
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Adult ,Marketing of Health Services ,Parents ,Young Adult ,Japan ,Humans ,Uterine Cervical Neoplasms ,Female ,Patient Acceptance of Health Care ,Early Detection of Cancer ,Nuclear Family - Abstract
In Japan, the rate of routine cervical cancer screening is quite low, and the incidence of cervical cancer has recently been increasing. Our objective was to investigate ways to effectively influence parental willingness to recommend that their 20-year-old daughters undergo cervical cancer screening.We targeted parents whose 20-year-old daughters were living with them. In fiscal year 2013, as usual, the daughter received a reminder postcard several months after they had received a free coupon for cervical cancer screening. In fiscal year 2014, the targeted parents received a cervical cancer information leaflet, as well as a cartoon about cervical cancer to show to their daughters, with a request that they recommend to their daughter that she undergo cervical cancer screening. The subsequent screening rates for fiscal years 2013 and 2014 were compared.The cervical cancer screening rate of 20-year-old women whose parents received the information packet in fiscal year 2014 was significantly higher than for the women who, in fiscal year 2013, received only a simple reminder postcard (P0.001). As a result, the total screening rate for 20-year-old women for the whole of the 2014 fiscal year was significantly increased over 2013 (P0.001).For the first time, we have shown that the parents of 20-year-old daughters can be motivated to recommend that their daughters receive their first cervical cancer screening. This was achieved by sending a cervical cancer information leaflet and a cartoon about cervical cancer for these parents to show to their daughters. This method was significantly effective for improving cervical cancer screening rates.
- Published
- 2016
68. Intense fluorodeoxyglucose uptake by a benign sclerosing stromal tumor of the ovary
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Takahiro Tsuboyama, Kazuya Mimura, Hiroki Kato, Yumiko Hori, Yayoi Fukuda, Takuji Tomimatsu, Kiyoshi Yoshino, and Tadashi Kimura
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Adult ,Pathology ,medicine.medical_specialty ,Sclerosing stromal tumor ,Ovary ,lcsh:Gynecology and obstetrics ,Diagnosis, Differential ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Fluorodeoxyglucose F18 ,Pregnancy ,Positron Emission Tomography Computed Tomography ,Obstetrics and Gynaecology ,medicine ,Humans ,Sex Cord-Gonadal Stromal Tumors ,lcsh:RG1-991 ,Positron Emission Tomography-Computed Tomography ,Fluorodeoxyglucose ,Ovarian Neoplasms ,030219 obstetrics & reproductive medicine ,business.industry ,Obstetrics and Gynecology ,Magnetic Resonance Imaging ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Female ,Radiopharmaceuticals ,business ,medicine.drug - Published
- 2016
69. Tumour-associated macrophages in diffuse large B-cell lymphoma: a study of the Osaka Lymphoma Study Group
- Author
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Hiroto Sakoda, Nobuhiko Tominaga, Mitsuru Tsudo, Jun Ishikawa, Hironori Take, Naoki Wada, Katsuyuki Aozasa, Machiko Tsukaguchi, Eiichi Morii, Yumiko Hori, Yoichi Tatsumi, Mona A A Zaki, Maki Kuwayama, and Koji Hashimoto
- Subjects
medicine.medical_specialty ,Pathology ,Histology ,business.industry ,CD68 ,General Medicine ,medicine.disease ,Gastroenterology ,Pathology and Forensic Medicine ,Lymphoma ,stomatognathic system ,Antigen ,Predictive value of tests ,Internal medicine ,Medicine ,Immunohistochemistry ,skin and connective tissue diseases ,business ,CD163 ,Survival rate ,Diffuse large B-cell lymphoma ,hormones, hormone substitutes, and hormone antagonists - Abstract
Wada N, Zaki M A A, Hori Y, Hashimoto K, Tsukaguchi M, Tatsumi Y, Ishikawa J, Tominaga N, Sakoda H, Take H, Tsudo M, Kuwayama M, Morii E & Aozasa K (2012) Histopathology 60, 313–319 Tumour-associated macrophages in diffuse large B-cell lymphoma: a study of the Osaka Lymphoma Study Group Aims: To evaluate the role of tumour-associated macrophages (TAMs) of the M1 and M2 types in the behaviour of diffuse large B-cell lymphoma (DLBCL). Methods and results: Double immunohistochemical staining of HLA-DR/CD68 (M1) or CD163/CD68 (M2) was performed in 101 cases of DLBCL. CD68+ cells represent the total number of TAMs. The average number of double-positive cells was counted, and the cut-off value was set at the mean number of counts, i.e. 30.7 and 27.0 for M1 TAMs and M2 TAMs, respectively. That for total TAMs was set at the 90th percentile number of total counts, i.e. 132.3. Cases were categorized into three pairs: high (34 cases) and low (67 cases) M1 TAM groups, high (39 cases) and low (62 cases) M2 TAM groups, and high (10 cases) and low (91 cases) total TAM groups. The difference in overall survival rates was statistically significant between the high and low M2 TAM groups (P
- Published
- 2011
70. Presence of B-cell clones in T-cell lymphoma
- Author
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Masaharu Kohara, Shigeki Fujita, Yumiko Hori, Jun-ichiro Ikeda, Eiichi Morii, Naoki Wada, Masayuki Hino, Yuzuru Kanakura, Hiroyasu Ogawa, Katsuyuki Aozasa, Mona A A Zaki, and Haruo Sugiyama
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Pathology ,medicine.medical_specialty ,Hematology ,General Medicine ,Biology ,medicine.disease ,medicine.disease_cause ,Epstein–Barr virus ,Virus ,law.invention ,Lymphoma ,medicine.anatomical_structure ,law ,hemic and lymphatic diseases ,Monoclonal ,medicine ,T-cell lymphoma ,Immunohistochemistry ,Polymerase chain reaction ,B cell - Abstract
Objectives: The presence of B-cell clones in 76 cases with peripheral T-cell lymphoma (PTCL) and precursor T-lymphoblastic lymphoma (T-LBL) and its correlation with Epstein–Barr virus (EBV) was studied. Methods: DNA was extracted from paraffin sections and/or fresh-frozen samples and then used for clonality analysis using a modified BIOMED-2 polymerase chain reaction (PCR)-based method. Results: T- and B-cell clones were detected in 59 (77.6%) and 14 (18.4%) of 76 patients, respectively: 90% and 30% of cases with PTCL, not otherwise specified, 76.4% and 17.6% of cases with angioimmunoblastic T-cell lymphoma, 77% and 7.6% of cases with adult T-cell lymphoma, 50% and 0% of cases with anaplastic large T-cell lymphoma, 62.5% and 12.5% of cases with T-LBL, and 50% and 0% of cases with intestinal T-cell lymphoma, respectively. Histological and immunohistochemical analyses revealed the presence of large B cells in lesional tissues, which were occasionally monoclonal. The presence of B-cell clones was highly associated with EBV positivity, as revealed by in situ hybridization. In two cases that were evaluated by serial histological and molecular examination, EBV-positive cells persisted in one and disappeared in the other. Conclusions: These findings suggest a role for EBV in the evolution of B-cell clones in T-cell lymphomas.
- Published
- 2011
71. Pulmonary Arterial Hypertension Associated with Chronic Active Epstein-Barr Virus Infection
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Yoshikazu Nakaoka, Katsuyuki Aozasa, Takahiro Hashimoto, Tetsuo Maeda, Yasushi Sakata, Wataru Shioyama, Yumiko Hori, Eiichi Morii, Yuzuru Kanakura, Kentaro Fukushima, Yoh Arita, Keiko Yamauchi-Takihara, and Issei Komuro
- Subjects
Male ,Epstein-Barr Virus Infections ,Chemotherapy ,Lung ,business.industry ,Chronic Active ,Hypertension, Pulmonary ,medicine.medical_treatment ,Autopsy ,General Medicine ,Middle Aged ,medicine.disease ,medicine.disease_cause ,Epstein–Barr virus ,Virus ,Natural killer cell ,Fatal Outcome ,medicine.anatomical_structure ,Heart failure ,Chronic Disease ,Immunology ,Internal Medicine ,Humans ,Medicine ,business - Abstract
A 45-year-old man with chronic active Epstein-Barr virus (EBV) infection (CAEBV) with natural killer cell type developed pulmonary arterial hypertension (PAH). After chemotherapy, he showed marked depression of the EBV DNA genome in the peripheral blood, but PAH sustained. He died of heart failure due to PAH, and the histo-pathological examination revealed pulmonary vascular abnormalities without lung disease on autopsy. Although the EBV DNA genome and the infiltrating lymphocytes were not detected in the lung, his clinical course suggested that his PAH might be caused by CAEBV. This is the first reported case of PAH associated CAEBV in an adult.
- Published
- 2011
72. Significance of aldehyde dehydrogenase 1 expression in stromal cells of diffuse large B-cell lymphoma
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Jun-ichiro Ikeda, Shigeki Fujita, Katsuyuki Aozasa, Eiichi Morii, Nur Rahadiani, Yumiko Hori, and Naoki Wada
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Cancer Research ,Stromal cell ,biology ,CD68 ,Cell ,Articles ,General Medicine ,medicine.disease ,Lymphoma ,medicine.anatomical_structure ,Immunology and Microbiology (miscellaneous) ,Cancer research ,medicine ,biology.protein ,Progenitor cell ,Lymph node ,Diffuse large B-cell lymphoma ,Fascin - Abstract
Aldehyde dehydrogenase (ALDH) 1 is expressed in various stem/progenitor cells, including cancer-initiating cells (CICs). In the present study, ALDH1 expression was immunohistochemically examined in diffuse large B-cell lymphoma (DLBCL). Unexpectedly, ALDH1 expression was occasionally detected in stromal cells, but not in lymphoma cells. Positively stained cells were CD68(+) cells with voluminous cytoplasm and fascin(+) cells with reticular morphology, designated as macrophages and dendritic cells (DCs), respectively. The presence of ALDH1(+) macrophages was not correlated with stage, response to therapies and prognosis. However, cases with ALDH1(+) DCs were mostly nodal, which showed a poorer response to therapies and a worse overall survival rate than cases without ALDH1(+) DCs. The presence of ALDH1(+) DCs appears to be involved in the malignant potential of DLBCL in lymph node.
- Published
- 2011
73. Abstract 5488: Serine racemase is a new therapeutic target for colon cancer
- Author
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Masako Kurashige, Eiichi Morii, Jun-ichiro Ikeda, Yumiko Hori, Satoshi Nojima, Kenji Ohshima, Keisuke Kawasaki, Naoki Wada, and Shinichiro Tahara
- Subjects
chemistry.chemical_classification ,Cancer Research ,Colorectal cancer ,Colon Adenoma ,Cell growth ,Biology ,medicine.disease ,digestive system diseases ,In vitro ,Amino acid ,Oncology ,chemistry ,Serine racemase ,Cancer cell ,Cancer research ,medicine ,Receptor - Abstract
Cancer cells have an altered metabolic state to meet the requirements of rapid proliferation, and altered amino acid metabolism is known to be significant for cancer cell growth. Amino acids consist of L- and D-amino acids, and humans are known to metabolize L-amino acids. D-amino acids have been considered not to exist in human body except in intestinal flora. Recently, D-serine was reported to be synthesized and catabolized by serine racemase (SRR) and D-amino acid oxidase, respectively, and it has an important function as co-activator of the N-methyl-D-aspartate receptor in the human brain. However, whether D-amino acids function in other human organs has not been elucidated. Moreover, the role of enzymes metabolizing D-amino acids in the context of cancer metabolism has not been studied. Here, we present a novel role for SRR in colon cancer. First, we analyzed SRR expression levels in colon adenoma, colon adenocarcinoma, and normal colon mucosa in ONCOMINE datasets. We found that SRR expression levels were significantly elevated in colon adenoma and colon adenocarcinoma compared to normal colon mucosa in several datasets, including that of the Cancer Genome Atlas (TCGA). To determine whether SRR has a functional role in colon cancer cells, we disrupted its expression using the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated 9 (Cas9) system in two colon cancer cell lines, HCT116 and DLD-1, using two distinct targets of short guide RNAs and examined the effects on cell proliferation. We found that SRR knockout resulted in reduced cell proliferation in both HCT116 and DLD-1 cells, which was recovered by exogenous SRR expression. In addition, a positive correlation was found between SRR expression levels and cell proliferation rate in four colon cancer cell lines, HCT116, DLD-1, WiDr, and Lovo. Moreover, phenazine methosulfate and L-aspartic acid β-hydroxamate, which have been previously reported to inhibit SRR, suppressed in vitro colon cancer cell proliferation. In conclusion, SRR is highly expressed in colon adenoma and colon adenocarcinoma compared to normal colon mucosa, and it enhances colon cancer cell proliferation. SRR is expected to be a potential new target for the development of novel therapies for colon cancer. Citation Format: Kenji Ohshima, Satoshi Nojima, Naoki Wada, Yumiko Hori, Shinichiro Tahara, Masako Kurashige, Keisuke Kawasaki, Junichiro Ikeda, Eiichi Morii. Serine racemase is a new therapeutic target for colon cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 5488.
- Published
- 2018
74. Novel phenolic glycosides, adenophorasides A–E, from Adenophora roots
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Toshiyuki Atsumi, Kazuo Toriizuka, Motonori Fukumura, Shiho Usui, Yuka Koike, Yumiko Hori, and Yasuaki Hirai
- Subjects
chemistry.chemical_classification ,Magnetic Resonance Spectroscopy ,Campanulaceae ,Molecular Structure ,biology ,Lobetyolin ,Traditional medicine ,Pharmacology toxicology ,Glycoside ,Alcohol ,biology.organism_classification ,Plant Roots ,Adenophora ,chemistry.chemical_compound ,chemistry ,Molecular Medicine ,Glycosides - Abstract
Five novel phenolic glycosides, adenophorasides A (1), B (2), C (3), D (4), and E (5), were isolated from commercial Adenophora roots, together with vanilloloside (6), 3,4-dimethoxybenzyl alcohol 7-O-beta-D: -glucopyranoside (7), and lobetyolin (8). The structures of the new compounds (1-5) were characterized as 4-hydroxy-3-methoxyphenylacetonitrile 4-O-beta-D: -glucopyranoside (1), 4-hydroxy-3-methoxyphenylacetonitrile 4-O-beta-D: -glucopyranosyl-(1--6)-beta-D: -glucopyranoside (2), 4-hydroxy-3-methoxyphenylacetonitrile 4-O-alpha-L: -rhamnopyranosyl-(1--6)-beta-D: -glucopyranoside (3), 4-hydroxyphenylacetonitrile 4-O-beta-D: -glucopyranosyl-(1--6)-beta-D: -glucopyranoside (4), and 4-hydroxy-3-methoxybenzyl alcohol 4-O-beta-D: -glucopyranosyl-(1--6)-beta-D: -glucopyranoside (5), respectively, by means of spectroscopic and chemical analyses.
- Published
- 2010
75. Lymphoproliferative disease of the kidney developing in fibro-inflammatory lesion
- Author
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Naoki Wada, Eiichi Morii, Tetsurou Yoshimoto, Masaharu Kohara, Yumiko Hori, Keisuke Kajio, and Katsuyuki Aozasa
- Subjects
Male ,Epstein-Barr Virus Infections ,Pathology ,medicine.medical_specialty ,Hyperlipidemias ,Abdominal cavity ,Granuloma, Plasma Cell ,Virus ,Pathology and Forensic Medicine ,Lesion ,hemic and lymphatic diseases ,Humans ,Medicine ,B cell ,Aged, 80 and over ,CD20 ,B-Lymphocytes ,Kidney ,biology ,business.industry ,Cell Biology ,Lymphoproliferative Disorders ,medicine.anatomical_structure ,Hypertension ,biology.protein ,Inflammatory pseudotumor ,Kidney Diseases ,medicine.symptom ,Lymphoproliferative disease ,business - Abstract
An 80-year-old man presented with continuous spike fever and night sweats. Computed tomographic scans revealed a poorly demarcated mass in the upper part of the right kidney, which was resected. At surgery, tumorous lesions were not found in the abdominal cavity. Serum IgG4 level measured after surgery was 40.1 mg/dl. Macroscopically, renal parenchyma of the upper part was replaced by an irregularly shaped grayish-white lesion of elastic, firm consistency. Histologically, the lesion consisted mostly of fibrous tissue in which small lymphoid cells, often with formation of aggregates, were evident. IgG4-positive plasma cells were few in number. Careful macroscopic examination revealed several minute nodules, which histologically consisted of large lymphoid cells, small lymphoid cells, and macrophages. These large lymphoid cells were positive for CD20 and contained Epstein-Barr virus (EBV) genome. Taken together, a diagnosis of EBV-positive B-cell lymphoproliferative disease (LPD) developing in inflammatory pseudotumor (IPT) of the kidney was made. This is the first report of B-LPD in IPT of the kidney. In addition, a presence of EBV in renal lymphoma cells has not yet been reported.
- Published
- 2010
76. Two new glucuronide saponins, Achyranthosides G and H, from Achyranthes fauriei root
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Yumiko Hori, Yasuaki Hirai, Yoshiteru Ida, Yoshiyuki Kuchino, Motonori Fukumura, Kazuo Toriizuka, and Hidehiro Ando
- Subjects
Magnetic Resonance Spectroscopy ,Molecular Structure ,biology ,Stereochemistry ,Saponins ,biology.organism_classification ,Plant Roots ,chemistry.chemical_compound ,Glucuronides ,chemistry ,Molecular Medicine ,Achyranthes ,Oleanolic Acid ,Glucuronide ,Oleanolic acid - Abstract
Two oleanolic acid saponins named achyranthosides G (1) and H (2) were newly isolated from Achyranthes fauriei root as methyl esters in addition to methyl esters of achyranthosides A - F and five oleanolic acid glucuronides (chikusetsusaponins IVa, V, 28-deglucosyl chikusetsusaponin V, pseudoginsenoside RT(1), and oleanolic acid 3-O-beta-D-glucuronopyranoside) as well as oleanolic acid 28-O-beta-D-glucopyranoside, beta-ecdysterone, and polypodine B. Their structures were characterized as follows on the basis of the chemical and spectroscopic evidences.
- Published
- 2007
77. The chemical constituents of fresh Gentian Root
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Mikio Fujii, Yoshiteru Ida, Yumiko Hori, Yasuaki Hirai, Kazuo Toriizuka, Toshiro Shibata, Hidehiro Ando, Motonori Fukumura, Yujiro Niiho, and Yoshijiro Nakajima
- Subjects
chemistry.chemical_classification ,Lignan ,Iridoid ,medicine.drug_class ,Pharmacology toxicology ,Glycoside ,Rhizome ,Dimethyl acetal ,chemistry.chemical_compound ,chemistry ,Chemical constituents ,Botany ,medicine ,Molecular Medicine ,Gentiana lutea - Abstract
We isolated and characterized 23 compounds, including a new iridoid named gentiolutelin and its dimethyl acetal, and a new lignan named gentioluteol from fresh roots (including small amounts of rhizome) of Gentiana lutea cultivated in Japan (Hokkaido). The structures of gentiolutelin and gentioluteol were determined as (1S,2R,3S,5R)-3-hydroxy-2-methyl-5-(2-oxoethyl)-cyclopentanecarboxylic acid methyl ester and (7R,8S,8′S)-4,4′,8,9-tetrahydroxy-3,3′,5-trimethoxy-7,9′-epoxylignan, respectively, on the basis of chemical and spectroscopic evidence. It was noteworthy that gentiopicroside, known to be a major secoiridoid glycoside in Gentian root, was not detected from the fresh roots of 3-year-old G. lutea.
- Published
- 2007
78. Similar protein expression profiles of ovarian and endometrial high-grade serous carcinomas
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Kiyoshi Yoshino, Shinya Matsuzaki, Eiichi Morii, Tetsuji Naka, Kosuke Hiramatsu, Takayuki Enomoto, Tadashi Kimura, Satoshi Serada, Yumiko Hori, Kosuke Yoshihara, Eiji Kobayashi, Yutaka Ueda, Tomomi Egawa-Takata, and Minoru Fujimoto
- Subjects
0301 basic medicine ,Adult ,Proteomics ,Cancer Research ,Pathology ,medicine.medical_specialty ,endocrine system diseases ,Biology ,Protein expression ,03 medical and health sciences ,0302 clinical medicine ,MCM2 ,Cell Line, Tumor ,Carcinoma ,medicine ,Biomarkers, Tumor ,Cluster Analysis ,Humans ,Pathological ,Molecular Diagnostics ,Aged ,Cell Proliferation ,Aged, 80 and over ,Ovarian Neoplasms ,Gene knockdown ,RNA-Binding Proteins ,Minichromosome Maintenance Complex Component 2 ,Middle Aged ,medicine.disease ,Immunohistochemistry ,female genital diseases and pregnancy complications ,Endometrial Neoplasms ,Serous fluid ,030104 developmental biology ,ovarian cancer ,Oncology ,iTRAQ ,Cell culture ,030220 oncology & carcinogenesis ,Gene Knockdown Techniques ,high-grade serous carcinoma ,endometrial cancer ,Cancer research ,Female ,IMP2 ,Neoplasms, Cystic, Mucinous, and Serous ,Carcinoma, Endometrioid - Abstract
Background: Ovarian and endometrial high-grade serous carcinomas (HGSCs) have similar clinical and pathological characteristics; however, exhaustive protein expression profiling of these cancers has yet to be reported. Methods: We performed protein expression profiling on 14 cases of HGSCs (7 ovarian and 7 endometrial) and 18 endometrioid carcinomas (9 ovarian and 9 endometrial) using iTRAQ-based exhaustive and quantitative protein analysis. Results: We identified 828 tumour-expressed proteins and evaluated the statistical similarity of protein expression profiles between ovarian and endometrial HGSCs using unsupervised hierarchical cluster analysis (P
- Published
- 2015
79. [Chemoradiotherapy is effective for primary esophageal adenosquamous cell carcinoma but ineffective for the metastatic adenocarcinoma component]
- Author
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Yasutoshi, Nozaki, Tsutomu, Nishida, Yumiko, Hori, Shinichiro, Shinzaki, Motohiko, Kato, Takayuki, Yakushijin, Hideki, Iijima, Masahiko, Tsujii, Eiichi, Morii, and Tetsuo, Takehara
- Subjects
Male ,Carcinoma, Adenosquamous ,Esophageal Neoplasms ,Humans ,Chemoradiotherapy ,Adenocarcinoma ,Middle Aged - Abstract
A 62-year-old man was referred to our hospital with dysphagia. Blood examination revealed significantly elevated serum CA19-9 levels but normal CEA and SCC levels. Imaging uncovered thoracic esophageal cancer with lung and bone metastasis, and subsequent endoscopic biopsy specimens of the primary esophageal tumor showed poorly differentiated squamous cell carcinoma. The patient underwent palliative chemoradiotherapy, but died due to progression of multiple metastases and increasing serum CA19-9 levels. Autopsy revealed adenocarcinoma in multiple metastatic foci, although the squamous component had disappeared in the primary and metastatic lesions. Therefore, we concluded that the adenocarcinoma component of adenosquamous cell carcinoma was refractory to chemoradiotherapy.
- Published
- 2015
80. Chemoenzymatic synthesis of optically active γ-alkyl-γ-butenolides
- Author
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Yumiko Hori, Yasuaki Hirai, Yoshiteru Ida, Kazuo Toriizuka, Kaoru Nakamura, Mikio Fujii, Hiroyuki Akita, and Motonori Fukumura
- Subjects
chemistry.chemical_classification ,Organic Chemistry ,Enantioselective synthesis ,Optically active ,Novozyme 435 ,Metathesis ,Catalysis ,Inorganic Chemistry ,chemistry ,Yield (chemistry) ,Organic chemistry ,Physical and Theoretical Chemistry ,Enantiomeric excess ,Alkyl - Abstract
rac-Hept-1-en-3-ol 4 was subjected to an enantioselective esterification in the presence of Novozyme 435 and vinyl crotonate as the acyl donor to give (3S)-oct-1-en-3-yl crotonate 7 in >99% ee and (3R)-alcohol 4 in 99% ee. The E-value of this enzymatic reaction was found to be >1000. The (S)-crotonic ester 7 was converted by ring-closing metathesis (RCM) using Grubbs’ catalyst to give (S)-oct-2-en-4-olide 1 in 96% yield while keeping the high enantiomeric excess.
- Published
- 2006
81. Poorly differentiated hepatocellular carcinoma accompanied by anti-Hu antibody-positive paraneoplastic peripheral neuropathy
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Takahiro, Matsui, Yumiko, Hori, Hiroaki, Nagano, Hidetoshi, Eguchi, Shigeru, Marubashi, Hiroshi, Wada, Naoki, Wada, Jun-Ichiro, Ikeda, Michiie, Sakamoto, and Eiichi, Morii
- Subjects
Diagnosis, Differential ,Carcinoma, Hepatocellular ,ELAV Proteins ,Paraneoplastic Syndromes ,Liver Neoplasms ,Humans ,Paraneoplastic Polyneuropathy ,Cell Differentiation ,Female ,Antibodies ,Aged - Abstract
The anti-Hu antibody is one of the most famous onco-neural antibodies related to paraneoplastic neurological syndrome, and is associated with small cell lung carcinoma in most cases. Here, we report a case of poorly differentiated hepatocellular carcinoma accompanied by paraneoplastic peripheral neuropathy positive for the anti-Hu antibody. Image inspection before operation revealed that no tumors were found in organs other than the liver, including lung, and that the liver tumor had no metastatic lesion. The liver tumor showed histological appearance of poorly differentiated carcinoma with cartilaginous metaplasia and partial blastoid cell appearance. Most tumor cells presented trabecular-like structure lined by sinusoidal vessels. Immunohistochemically, the tumor cells were positive for low molecular weight cytokeratin and vimentin, partially positive for cytokeratin 19 and CD56, but negative for synaptophysin, chromogranin A and alpha-fetoprotein. Based on the trabecular-like morphology and the results of immunohistochemical staining, we concluded that the tumor was diagnosed as poorly differentiated hepatocellular carcinoma. Anti-Hu antibody-positive paraneoplastic peripheral neuropathy accompanied with liver tumor is extremely rare as far as is known. The presented case indicates that poorly differentiated carcinoma has the potential to be the responsible lesion of anti-Hu antibody-positive paraneoplastic neurological syndrome and systemic work-up is important for the management of this neurological disorder.
- Published
- 2014
82. Laparoscopic excisional surgery for growing teratoma syndrome of the ovary: case report and literature review
- Author
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Kenjiro Sawada, Kiyoshi Yoshino, Eiji Kobayashi, Yumiko Hori, Tadashi Kimura, Yutaka Ueda, and Naoya Shigeta
- Subjects
Laparoscopic surgery ,Adult ,medicine.medical_specialty ,endocrine system diseases ,medicine.medical_treatment ,Ovariectomy ,Malignant Germ Cell Tumor ,Paracolic gutters ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Biomarkers, Tumor ,Humans ,Laparoscopy ,Neoplasm Staging ,Ovarian Neoplasms ,Chemotherapy ,medicine.diagnostic_test ,business.industry ,Teratoma ,Obstetrics and Gynecology ,medicine.disease ,Surgery ,medicine.anatomical_structure ,Treatment Outcome ,Immature teratoma ,Female ,Pouch ,Neoplasm Recurrence, Local ,business - Abstract
Growing teratoma syndrome (GTS) is rare clinical phenomenon occurring as a sequelae of a malignant germ cell tumor. We present the case of a 20-year-old woman who developed GTS after undergoing fertility-sparing surgery and chemotherapy for an immature teratoma. She underwent left salpingo-oophorectomy, right ovarian cystectomy, and disseminated tumor reduction during her primary surgery. The postsurgical histology report identified the tumor as an immature teratoma, grade 3, International Federation of Gynecology and Obstetrics (FIGO) stage IIIb. She subsequently received 3 cycles of chemotherapy consisting of bleomycin, etoposide, and cisplatin. At 17 months after the chemotherapy, follow-up computed tomography (CT) scan revealed an enlarged mass in her right paracolic gutter and a small peritoneal lesion in the pouch of Douglas. Her serum alpha-fetoprotein level was not elevated. These findings were compatible with GTS, but it was difficult to rule out a recurrent immature teratoma. Diagnostic exploratory laparoscopic surgery revealed the enlarged tumors that had been detected by the CT scan. Although there were multiple tumors in the pouch of Douglas, we were able to resect all of them laparoscopically. Histological diagnosis of the surgically resected specimens was of a mature teratoma, and so we concluded that this tumor was a GTS. Our experience suggests that laparoscopic surgery is an effective alternative diagnostic and therapeutic approach in cases suspicious of GTS where the disease is disseminated to the peritoneum.
- Published
- 2014
83. Pharmacognostic studies on ginger and related drugs—part 1: five sulfonated compounds from Zingiberis rhizome (Shokyo)
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Tsuyoshi Miura, Yumiko Hori, Yasuaki Hirai, Yukio Nemoto, Motonori Fukumura, Kazuo Toriizuka, and Yoshiteru Ida
- Subjects
China ,Magnetic Resonance Spectroscopy ,Pharmacognosy ,Traditional medicine ,Chemistry ,Plant Science ,General Medicine ,Ginger ,Horticulture ,Biochemistry ,Rhizome ,Botany ,Sulfonic Acids ,Molecular Biology - Abstract
Five sulfonated compounds, namely 4-gingesulfonic acid and shogasulfonic acids A, B, C and D, were isolated together with seven known compounds including 6-gingesulfonic acid from Zingiberis rhizome (Japanese name: Shokyo) made out of ginger. Their structures were characterized by means of spectroscopic analysis.
- Published
- 2003
84. Structural Revision of a Naphthodipyranodione from Gentian Root and its Degradation Pathway from Gentiopicroside
- Author
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Mikio Fujii, Yasuaki Hirai, Kiju Konno, Hidehiro Ando, Motonori Fukumura, Yoshiteru Ida, and Yumiko Hori
- Subjects
Pharmacology ,Gentianaceae ,Chromatography ,biology ,010405 organic chemistry ,Chemistry ,Plant Science ,General Medicine ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Complementary and alternative medicine ,Enzymatic hydrolysis ,Drug Discovery ,Degradation (geology) ,Fermentation ,Degradation pathway - Abstract
Based on NOE experiments, the structure of naphthodipyranodione from Gentianaceae plants was revised to 1,2-dihydro-4 H,6 H,8 H-naphto[1,2-d:4,5-c'd']dipyrano-4,8-dione. Naphthodipyranodione was assumed to be formed by the degradation of gentiopicroside by enzymatic hydrolysis at low water concentration. The degradation pathway was a unique domino-reaction triggered by enzymatic hydrolysis. Naphthodipyranodione may become an index compound for the drying and/or fermenting procedure of Gentian root.
- Published
- 2017
85. Assessment of combination of contrast-enhanced magnetic resonance imaging and positron emission tomography/computed tomography for evaluation of ovarian masses
- Author
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Mitsuaki Tatsumi, Tonsok Kim, Atsushi Nakamoto, Masatoshi Hori, Jun Hatazawa, Takahiro Tsuboyama, Hiromitsu Onishi, Yumiko Hori, Noriyuki Tomiyama, Makoto Sakane, and Eiichi Morii
- Subjects
Adult ,Contrast Media ,Gadolinium ,Malignancy ,Multimodal Imaging ,Sensitivity and Specificity ,Fluorodeoxyglucose F18 ,Heterocyclic Compounds ,Image Interpretation, Computer-Assisted ,medicine ,Organometallic Compounds ,Humans ,Radiology, Nuclear Medicine and imaging ,Contrast-enhanced Magnetic Resonance Imaging ,Computed tomography laser mammography ,Positron Emission Tomography-Computed Tomography ,Aged ,Fluorodeoxyglucose ,Aged, 80 and over ,Ovarian Neoplasms ,medicine.diagnostic_test ,business.industry ,Reproducibility of Results ,General Medicine ,Middle Aged ,medicine.disease ,Image Enhancement ,Magnetic Resonance Imaging ,Positron emission tomography ,Positron-Emission Tomography ,Female ,Tomography ,Radiopharmaceuticals ,business ,Nuclear medicine ,Tomography, X-Ray Computed ,Preclinical imaging ,medicine.drug - Abstract
The objectives of this study were to correlate fluorodeoxyglucose uptake in ovarian masses on positron emission tomography/computed tomography (PET/CT) with pathological grades of malignancy and subtypes and to determine the appropriate approach for combining PET/CT and contrast-enhanced magnetic resonance imaging (CE-MRI) to characterize ovarian masses.A retrospective study was conducted including 127 patients who underwent surgical resection of an ovarian mass (30 benign, 31 borderline, 66 malignant). Maximum standardized uptake values (SUVmax) obtained with PET/CT were compared between pathological grades of malignancy and subtypes. Two radiologists each independently conducted a blind evaluation of CE-MRI for all lesions and classified them by the grade of malignancy as determinate (benign, borderline, or malignant) or indeterminate and by subtype as mucinous or nonmucinous. The appropriate approach for combining CE-MRI and PET/CT was determined by comparing the combined diagnostic ability with that of CE-MRI alone.The SUVmax of malignant tumors was significantly higher than that of benign and borderline lesions (mean, 7.8, 1.7, 2.4; P0.05). Among malignant tumors, SUVmax was significantly lower in mucinous adenocarcinomas compared with nonmucinous malignant tumors (mean, 3.3, 8.4; P0.05) and lower in clear cell adenocarcinomas compared with other subtypes of nonmucinous malignant tumors (mean, 6.0, 9.4; P0.05). The SUVmax cutoff that best differentiated malignant lesions from benign/borderline lesions was 2.4 for mucinous and 4.0 for nonmucinous tumors. These cutoffs correctly classified lesions as malignant or not in 88.2% of cases (112/127). When PET/CT was combined with CE-MRI, the readers correctly classified 85% (34/40) and 86.5% (32/37) of indeterminate lesions on CE-MRI. However, PET/CT was not useful for classifying determinate lesions on CE-MRI, particularly because PET/CT correctly classified only 70.1% (12/17) of clear cell adenocarcinomas, whereas CE-MRI alone correctly classified 94.1% (1617). Thus, compared with CE-MRI alone, the diagnostic accuracy of CE-MRI + PET/CT when PET/CT was added only for indeterminate lesions on CE-MRI was significantly higher for both readers for differentiating between benign and borderline/malignant (P0.05), as well as between benign/borderline and malignant (P0.01).Fluorodeoxyglucose uptake in ovarian masses correlates with pathological subtypes as well as the grade of malignancy. Furthermore, the combination of CE-MRI and PET/CT is a highly accurate method for characterizing ovarian masses because PET/CT can be used as a complement to classify indeterminate lesions as malignant or not based on appropriate cutoff SUVmax for mucinous and nonmucinous tumors.
- Published
- 2014
86. Embolic effects of transcatheter mesenteric arterial embolization with microspheres on the small bowel in a dog model
- Author
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Yoshiyuki Higashi, Eiichi Morii, Masahisa Nakamura, Yumiko Hori, Fumihito Ohashi, Noboru Maeda, Keigo Osuga, Akiyoshi Hayashi, Kentaro Kishimoto, and Noriyuki Tomiyama
- Subjects
medicine.medical_specialty ,medicine.medical_treatment ,Dog model ,Microsphere ,Dogs ,Mesenteric Artery, Superior ,medicine.artery ,Intestine, Small ,medicine ,Animals ,Radiology, Nuclear Medicine and imaging ,Embolization ,Superior mesenteric artery ,Mesentery ,business.industry ,Significant difference ,Vasa recta ,Mesenteric arterial embolization ,Embolization, Therapeutic ,Microspheres ,Radiography ,medicine.anatomical_structure ,Models, Animal ,Radiology ,Cardiology and Cardiovascular Medicine ,business - Abstract
To determine the arterial distribution and ischemic effects of various particle sizes after transcatheter embolization of the small bowel in a dog model.In 10 dogs, selective microsphere embolization was performed in six branches of the superior mesenteric artery. Microspheres were allocated into three size ranges (100-300 μm, 300-500 μm, and 500-700 μm) and four volume concentrations (0.625%, 1.25%, 2.5%, and 5%). For each size and volume concentration, embolization was performed of five branches at the origin of the last arcade. The distribution of microspheres and the range of ischemic changes of mucosa were evaluated histologically. Angiograms were categorized into two groups: group A, only the vasa recta nonopacified; group B, the last arcade or more proximal branches nonopacified.Microspheres sized 100-300 μm penetrated into intramural arteries and 500-700 μm microspheres mainly blocked arteries in the mesentery. There was a significant difference among three sizes in terms of the locations within the vasculature (P.0001). The larger volume and the smaller size resulted in more ischemia. The range of ischemic changes among three sizes and among four volume concentrations was significantly different (P = .004 and P.0001, respectively). The range of ischemic changes with 500-700 μm microspheres in group B was significantly greater than in group A (0% in group A vs 83% in group B, P = .001).In a dog model, embolization of the small bowel limited to the vasa recta with the use if 500-700 μm microspheres reduced the range of ischemic changes.
- Published
- 2014
87. In vivo evaluation of irinotecan-loaded QuadraSphere microspheres for use in chemoembolization of VX2 liver tumors
- Author
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Fumihito Ohashi, Eiichi Morii, Keigo Osuga, Yoshiyuki Higashi, Akiyoshi Hayashi, Kentaro Kishimoto, Yumiko Hori, Kaishu Tanaka, Noriyuki Tomiyama, and Noboru Maeda
- Subjects
medicine.medical_specialty ,Liver tumor ,Colorectal cancer ,medicine.medical_treatment ,Ioxaglic acid ,Urology ,Irinotecan ,Hepatic arterial infusion ,Liver Neoplasms, Experimental ,Pharmacokinetics ,In vivo ,Medicine ,Animals ,Radiology, Nuclear Medicine and imaging ,Embolization ,Chemoembolization, Therapeutic ,business.industry ,medicine.disease ,Antineoplastic Agents, Phytogenic ,Microspheres ,Disease Models, Animal ,Treatment Outcome ,Anesthesia ,Camptothecin ,Female ,Rabbits ,Cardiology and Cardiovascular Medicine ,business ,Tomography, X-Ray Computed ,medicine.drug - Abstract
Purpose To investigate the pharmacokinetics and chemoembolization efficacy of irinotecan-loaded QuadraSphere microspheres (QSMs) in a rabbit VX2 liver tumor model. Materials and Methods Fourteen rabbits with VX2 liver tumors were divided into two groups. In the irinotecan-loaded QSM group (n = 7), 3 mg of QSMs (30–60 μm) containing 12 mg of irinotecan (0.6 mL; 20 mg/mL) were injected into the left hepatic artery. In the control group (hepatic arterial infusion [HAI] and QSMs; n=7), 3 mg of QSMs suspended in ioxaglic acid were injected following a bolus injection of 0.6 mL of irinotecan solution (20 mg/mL). Sequential irinotecan, SN-38, and SN-38G concentration changes were measured in plasma within 24 hours and at 1 week and in tissues at 1 week. The VX2 tumor growth rates at 1 and 2 weeks were calculated from computed tomographic images. Results All rabbits underwent successful embolization. Plasma irinotecan, SN-38, and SN-38G concentrations in the irinotecan-loaded QSM group showed significantly sustained release compared with the control group ( P = .01). Compared with the control group, the irinotecan-loaded QSM group had significantly higher irinotecan concentration in liver tumors ( P = .03) and a tendency toward higher SN-38 concentration in liver tumors ( P = .29). The SN-38G tissue concentrations were below the limits of quantification. The tumor growth rate was significantly lower and the tumor necrosis rate significantly higher in the irinotecan-loaded QSM group ( P = .02 and P = .01, respectively). Conclusion Chemoembolization via irinotecan-loaded QSMs more effectively suppresses tumor growth than chemoembolization with unloaded QSMs after HAI. A clinical feasibility study is warranted.
- Published
- 2014
88. Interaction of mastoparan with membranes studied by1H-NMR spectroscopy in detergent micelles and by solid-state2H-NMR and15N-NMR spectroscopy in oriented lipid bilayers
- Author
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Mitsuo Iwadate, Haruhiko Aoyagi, Takuro Niidome, Anne S. Ulrich, Makoto Demura, Tetsuo Asakura, and Yumiko Hori
- Subjects
Crystallography ,Chemistry ,Bilayer ,Chemical shift ,Mastoparan ,Helix ,Analytical chemistry ,Nuclear magnetic resonance spectroscopy ,Lipid bilayer ,complex mixtures ,Biochemistry ,Two-dimensional nuclear magnetic resonance spectroscopy ,Micelle - Abstract
Several complementary NMR approaches were used to study the interaction of mastoparan, a 14-residue peptide toxin from wasp venom, with lipid membranes. First, the 3D structure of mastoparan was determined using 1H-NMR spectroscopy in perdeuterated (SDS-d25) micelles. NOESY experiments and distance geometry calculations yielded a straight amphiphilic α-helix with high-order parameters, and the chemical shifts of the amide protons showed a characteristic periodicity of 3–4 residues. Secondly, solid-state 2H-NMR spectoscopy was used to describe the binding of mastoparan to lipid bilayers, composed of headgroup-deuterated dimyristoylglycerophosphocholine (DMPC-d4) and dimyristoylphosphatidylglycerol (DMPG). By correlating the deuterium quadrupole splittings of the α-segments and β-segments, it was possible to differentiate the electrostatically induced structural response of the choline headgroup from dynamic effects induced by the peptide. A partial phase separation was observed, leading to a DMPG-rich phase and a DMPG-depleted phase, each containing some mastoparan. Finally, the insertion and orientation of a specifically 15N-labeled mastoparan (at position Ala10) in the bilayer environment was investigated by solid-state 15N-NMR spectroscopy, using macroscopically oriented samples. Two distinct orientational states were observed for the mastoparan helix, namely an in-plane and a trans-membrane alignment. The two populations of 90% in-plane and 10% trans-membrane helices are characterized by a mosaic spread of ± 30° and ± 10°, respectively. The biological activity of mastoparan is discussed in terms of a pore-forming model, as the peptide is known to be able to induce nonlamellar phases and facilitate a flip-flop between the monolayers.
- Published
- 2001
89. Histologic Findings in a Dilated Atrium Serving as a Fontan Pathway for 23 Years
- Author
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Takayoshi Ueno, Hajime Ichikawa, Iki Adachi, Yumiko Hori, Toru Kuratani, Goro Matsumiya, and Yoshiki Sawa
- Subjects
Adult ,Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Time Factors ,Fontan pathway ,medicine.medical_treatment ,Economic shortage ,Interstitial fibrosis ,Fontan Procedure ,Muscle hypertrophy ,Fontan procedure ,Internal medicine ,medicine ,Humans ,Heart Atria ,cardiovascular diseases ,Atrium (heart) ,Dilated atrium ,business.industry ,Surgery ,medicine.anatomical_structure ,Circulatory system ,cardiovascular system ,Cardiology ,Female ,Cardiology and Cardiovascular Medicine ,business - Abstract
We microscopically examined atrial tissues surgically obtained from a 35-year-old woman who had been palliated with the atriopulmonary connection in a Fontan operation 23 years earlier. Apart from mild hypertrophy of myocytes and trivial interstitial fibrosis, no gross abnormalities were identified. Although this finding is somehow surprising, considering the duration of atrial pressure-loading, it seems to be in accordance with the result of her Holter recordings, which demonstrates the absence of any rhythm disturbances other than sporadic premature contractions. Because of the shortage of histologic investigations in this group of patients, we present this case with a review of the literature.
- Published
- 2010
90. Orientational behavior of phospholipid membranes with mastoparan studied by 31 P solid state NMR
- Author
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Haruhiko Aoyagi, Makoto Demura, Yumiko Hori, Takuro Niidome, and Tetsuo Asakura
- Subjects
Magnetic Resonance Spectroscopy ,Lipid Bilayers ,Biophysics ,Phospholipid ,Membrane orientation ,Wasp Venoms ,Peptide ,Biochemistry ,Nuclear magnetic resonance ,chemistry.chemical_compound ,Non-lamellar phase ,Structural Biology ,Genetics ,Animals ,Lamellar structure ,Amino Acid Sequence ,Anisotropy ,Lipid bilayer ,Molecular Biology ,chemistry.chemical_classification ,Mastoparan ,Phosphatidylglycerols ,Phosphorus ,Cell Biology ,Crystallography ,Membrane ,chemistry ,Solid-state nuclear magnetic resonance ,Intercellular Signaling Peptides and Proteins ,lipids (amino acids, peptides, and proteins) ,Dimyristoylphosphatidylcholine ,Peptides - Abstract
Solid state 31P NMR spectroscopy was used to study the perturbing effect of the wasp venom peptide mastoparan (MP) on lipid bilayers composed of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylglycerol (DMPG). The 31P chemical shift anisotropy of multilamellar vesicles decreased with increasing peptide concentration, indicating that MP interacts strongly and selectively with the charged DMPG head group. Macroscopically oriented MP-lipid samples between glass plates were studied by 31P NMR as a function of tilt angle. These spectra showed the coexistence of orientation-dependent lamellar signals as well as an isotropic peak, suggesting that MP can induce non-lamellar phases in DMPC/DMPG membranes.
- Published
- 1999
91. Inhibitory effect of Nodal on the expression of aldehyde dehydrogenase 1 in endometrioid adenocarcinoma of uterus
- Author
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Yumiko Hori, Yang Jiang, Yi Wang, Eiichi Morii, Naoki Wada, Jun-ichiro Ikeda, and Tian Tian
- Subjects
medicine.medical_specialty ,Nodal Protein ,Population ,Biophysics ,Uterus ,Aldehyde dehydrogenase ,Biology ,Biochemistry ,Aldehyde Dehydrogenase 1 Family ,Transforming Growth Factor beta ,Internal medicine ,Cell Line, Tumor ,medicine ,Endometrioid adenocarcinoma ,Humans ,education ,Molecular Biology ,education.field_of_study ,Ubiquitination ,Retinal Dehydrogenase ,Cell Biology ,Endometrial Neoplasms ,Isoenzymes ,medicine.anatomical_structure ,Endocrinology ,Cell culture ,Proteolysis ,Cancer research ,biology.protein ,Immunohistochemistry ,Female ,NODAL ,Carcinoma, Endometrioid ,Transforming growth factor - Abstract
Cancers consist of heterogeneous populations. Recently, it has been demonstrated that cells with tumorigenic potential are limited to a small population, called cancer-initiating cells (CICs). Aldehyde dehydrogenase 1 (ALDH1) is one of the markers of CICs. We previously reported that ALDH1-high cases of uterine endometrioid adenocarcinoma showed poor prognosis, and ALDH1-high population of endometrioid adenocarcinoma cell line was more tumorigenic, resistant to anti-cancer drugs, and invasive than ALDH1-low population. Here, the regulatory signaling for ALDH1 was examined. The inhibition of TGF-β signaling increased ALDH1-high population. Among TGF-β family members, Nodal expression and ALDH1 expression levels were mutually exclusive. Immunohistochemical analysis on clinical samples revealed Nodal-high tumor cells to be ALDH-low and vise versa, suggesting that Nodal may inhibit ALDH1 expression via stimulating TGF-β signaling in uterine endometrioid adenocarcinoma. In fact, the addition of Nodal to endometrioid adenocarcinoma cell line reduced ALDH1-high population. Although ALDH1 mRNA level was not affected, the amount of ALDH1 protein appeared to be reduce by Nodal through ubiquitine-proteasome pathway. The regulation of TGF-β signaling might be a novel therapeutic target of CICs in endometrioid adenocarcinoma.
- Published
- 2013
92. [Heart transplantation and ventricular assit systems]
- Author
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Takeshi, Nakatani, Hiroki, Hata, Tomoyuki, Fujita, Junjiro, Kobayashi, Yoshihiro, Murata, Osamu, Seguchi, Masanobu, Yanase, Yumiko, Hori, Kyoichi, Wada, Hatsue, Ueda, Shigeki, Miyata, and Hiroaki, Naito
- Subjects
Adult ,Heart Failure ,Male ,Adolescent ,Heart Transplantation ,Humans ,Female ,Heart-Assist Devices ,Middle Aged - Abstract
Since the organ transplantation law was passed, we performed 50 heart transplantation at National Cerebral and Cardiovascular Center. Of those, 2 patients have been doing well over 13 years and 10 years survival rate was 93.4%.During those years, we performed 139 applications of left ventricular assist systems( LVAS). Initially, extracorporeal LVASs had been used. Now, 2 implantable LVAS were approved by medical insurance as bridge to transplant in 2011. Now, our 1st option as bridge to transplantation(BTT) is implantable LVAS.
- Published
- 2013
93. Characterization of subpopulation lacking both B-cell and plasma cell markers in Waldenstrom macroglobulinemia cell line
- Author
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Maosheng Zhan, Jun-ichiro Ikeda, Eiichi Morii, Yumiko Hori, Naoki Wada, and Keiichiro Honma
- Subjects
Apoptosis ,Plasma cell ,Biology ,Pathology and Forensic Medicine ,Immunophenotyping ,immune system diseases ,hemic and lymphatic diseases ,Cell Line, Tumor ,medicine ,Humans ,Molecular Biology ,B cell ,Cellular Senescence ,CD20 ,B-Lymphocytes ,Forkhead Box Protein O3 ,Waldenstrom macroglobulinemia ,Forkhead Transcription Factors ,Cell Biology ,medicine.disease ,Antigens, CD20 ,Flow Cytometry ,Molecular biology ,medicine.anatomical_structure ,Phenotype ,Cell culture ,Cancer cell ,biology.protein ,Syndecan-1 ,Waldenstrom Macroglobulinemia ,Reactive Oxygen Species ,Cell aging ,Biomarkers - Abstract
Cancer cells with tumorigenic potential are limited to a small population known as cancer-initiating cells (CICs). To date, CICs have not been identified in non-Hodgkin's lymphomas. Here, we investigated a candidate of CICs of an indolent non-Hodgkin's lymphoma, Waldenstrom macroglobulinemia (WM), using WM cell line MWCL-1. WM tumor expresses both B-cell and plasma cell markers, CD20 and CD138. When stained with anti-CD20 and anti-CD138 antibodies, MWCL-1 cells were classified into three subpopulations: CD20⁻ CD138⁻, CD20⁺ CD138⁻, and CD20⁺ CD138⁺. When cultured, CD20⁻ CD138⁻ cells yielded all three subpopulations, but CD20⁺ cells did not yield CD20⁻ CD138⁻ cells. Higher reactive oxygen species (ROS) expelling and in vitro colony formation activities were detected in CD20⁻ CD138⁻ cells than in CD20⁺ CD138⁻ and CD20⁺ CD138⁺ cells. When cultured in the absence of serum or with anti-cancer drug, CD20⁻ CD138⁻ cells were resistant to apoptosis. In contrast, CD20⁺ CD138⁺ cells were vulnerable to apoptosis in the same condition. In fact, the immunohistochemical analysis with clinical samples revealed that tumor cells in apoptosis were CD138-positive. The production of all three subpopulations, the efficient ROS expelling and in vitro colony-forming activities, and the resistance to apoptosis suggested that the CD20⁻ CD138⁻ cell might be a candidate of CICs in WM.
- Published
- 2013
94. Independent association between low serum amylase and non-alcoholic fatty liver disease in asymptomatic adults: a cross-sectional observational study
- Author
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Hiroshi Fuchigami, Toshitaka Muneyuki, Kei Nakajima, Haruki Oshida, Hiromi Munakata, Yumiko Hori, Masafumi Kakei, and Masafumi Saito
- Subjects
medicine.medical_specialty ,Pathology ,Epidemiology ,Non-obesity ,Gastroenterology ,Asymptomatic ,Insulin resistance ,Diabetes mellitus ,Internal medicine ,NAFLD ,medicine ,Clinical Epidemiology ,Obesity ,Cross sectional studies ,business.industry ,Research ,Fatty liver ,General Medicine ,medicine.disease ,Quartile ,Serum amylase ,Metabolic syndrome ,medicine.symptom ,business ,Body mass index - Abstract
OBJECTIVES: Low serum amylase (LSA) was reported to be associated with obesity, metabolic syndrome (MetS) and diabetes. However, it is unknown as to whether LSA is associated with non-alcoholic fatty liver disease (NAFLD), a hepatic manifestation of MetS and insulin resistance. Therefore, we performed a clinical epidemiological study to investigate this potential association. DESIGN: A cross-sectional observational study with multivariate analysis. SETTING: Subjects were recruited in a healthcare centre in Saitama, an eastern district of Japan, near Tokyo. PARTICIPANTS: A total of 1475 asymptomatic adults aged 30-79 years who underwent detailed medical check-ups and who regularly consumed small amounts of alcohol (, Free PMC Article. Open Access.
- Published
- 2013
95. Pedunculated Sub-Serous Leiomyosarcoma Mimicking Ovarian Cancer: Case Report and Review of Literature
- Author
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Takuhei Yokoyama, Kiyoshi Yoshino, Yutaka Ueda, Masami Fujita, Yumiko Hori, Satoshi Nakagawa, Eiichi Morii, Shinya Matsuzaki, Toshihiro Kimura, and Tadashi Kimura
- Subjects
Leiomyosarcoma ,Pathology ,medicine.medical_specialty ,Malignant Ovarian Tumor ,business.industry ,Uterine leiomyosarcoma ,Uterine fundus ,medicine.disease ,body regions ,Serous fluid ,Medicine ,Radiology ,Differential diagnosis ,business ,Ovarian cancer - Abstract
Pedunculated subserous leiomyosarcoma is a quite rare presentation of leiomyosarcoma. As of 2013, only three cases have been reported in the literature. In this case report, we document two new cases of pedunculated subserous leiomyosarcoma from uterine fundus. These two cases illustrate the difficulty of making a correct differential diagnosis between a pedunculatedsubserous uterine leiomyosarcoma and a malignant ovarian tumor before intervention. A review of the literature confirms that this site remains unusual and making the diagnosis is difficult.
- Published
- 2013
96. Prediabetes and impaired lung function in asymptomatic adults
- Author
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Hiroshi Fuchigami, Satoshi Tobimatsu, Kei Nakajima, Haruki Oshida, Hiromi Munakata, Masafumi Saito, Yulan Li, and Yumiko Hori
- Subjects
Adult ,Blood Glucose ,Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Physiology ,Asymptomatic ,Impaired glucose tolerance ,Prediabetic State ,Endocrinology ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,medicine ,Humans ,Prediabetes ,Lung ,Lung function ,business.industry ,Confounding ,General Medicine ,Middle Aged ,medicine.disease ,Impaired fasting glucose ,Cross-Sectional Studies ,Spirometry ,Female ,medicine.symptom ,Metabolic syndrome ,business ,Lung Volume Measurements - Abstract
Diabetes and metabolic syndrome are associated with impaired lung function. However, it is unknown whether this is also true in prediabetes. In a cross-sectional study of 1237 asymptomatic adults, we found that diabetes and prediabetes were both significantly associated with low vital capacity, even after adjustment for relevant confounding factors.
- Published
- 2012
97. Tumour-associated macrophages in diffuse large B-cell lymphoma: a study of the Osaka Lymphoma Study Group
- Author
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Naoki, Wada, Mona A A, Zaki, Yumiko, Hori, Koji, Hashimoto, Machiko, Tsukaguchi, Yoichi, Tatsumi, Jun, Ishikawa, Nobuhiko, Tominaga, Hiroto, Sakoda, Hironori, Take, Mitsuru, Tsudo, Maki, Kuwayama, Eiichi, Morii, and Katsuyuki, Aozasa
- Subjects
Adult ,Aged, 80 and over ,Male ,Adolescent ,Macrophages ,Antigens, Differentiation, Myelomonocytic ,Middle Aged ,Prognosis ,Survival Rate ,Young Adult ,Japan ,Antigens, CD ,Predictive Value of Tests ,Humans ,Female ,Lymphoma, Large B-Cell, Diffuse ,Aged ,Retrospective Studies - Abstract
To evaluate the role of tumour-associated macrophages (TAMs) of the M1 and M2 types in the behaviour of diffuse large B-cell lymphoma (DLBCL).Double immunohistochemical staining of HLA-DR/CD68 (M1) or CD163/CD68 (M2) was performed in 101 cases of DLBCL. CD68+ cells represent the total number of TAMs. The average number of double-positive cells was counted, and the cut-off value was set at the mean number of counts, i.e. 30.7 and 27.0 for M1 TAMs and M2 TAMs, respectively. That for total TAMs was set at the 90th percentile number of total counts, i.e. 132.3. Cases were categorized into three pairs: high (34 cases) and low (67 cases) M1 TAM groups, high (39 cases) and low (62 cases) M2 TAM groups, and high (10 cases) and low (91 cases) total TAM groups. The difference in overall survival rates was statistically significant between the high and low M2 TAM groups (P0.01) and between the high and low total TAM groups (P0.05). Multivariate analysis revealed that the presence of a bulky mass and a higher number of M2 TAMs were significant factors for poor prognosis (P0.05).Estimation of specific type of macrophages, of the M1 and M2 types, is superior to the estimation of TAMs as a whole (CD68+ cells) for prediction of the prognosis of DLBCL patients.
- Published
- 2012
98. Presence of B-cell clones in T-cell lymphoma
- Author
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Mona A A, Zaki, Naoki, Wada, Masaharu, Kohara, Junichiro, Ikeda, Yumiko, Hori, Shigeki, Fujita, Hiroyasu, Ogawa, Haruo, Sugiyama, Masayuki, Hino, Yuzuru, Kanakura, Eiichi, Morii, and Katsuyuki, Aozasa
- Subjects
Adult ,Aged, 80 and over ,Male ,B-Lymphocytes ,Herpesvirus 4, Human ,Adolescent ,Middle Aged ,Gene Rearrangement, T-Lymphocyte ,Lymphoma, T-Cell ,Prognosis ,Polymerase Chain Reaction ,Clone Cells ,Young Adult ,Humans ,Female ,Gene Rearrangement, B-Lymphocyte ,In Situ Hybridization ,Aged - Abstract
The presence of B-cell clones in 76 cases with peripheral T-cell lymphoma (PTCL) and precursor T-lymphoblastic lymphoma (T-LBL) and its correlation with Epstein-Barr virus (EBV) was studied. DNA was extracted from paraffin sections and/or fresh-frozen samples and then used for clonality analysis using a modified BIOMED-2 polymerase chain reaction (PCR)-based method. T- and B-cell clones were detected in 59 (77.6%) and 14 (18.4%) of 76 patients, respectively: 90% and 30% of cases with PTCL, not otherwise specified, 76.4% and 17.6% of cases with angioimmunoblastic T-cell lymphoma, 77% and 7.6% of cases with adult T-cell lymphoma, 50% and 0% of cases with anaplastic large T-cell lymphoma, 62.5% and 12.5% of cases with T-LBL, and 50% and 0% of cases with intestinal T-cell lymphoma, respectively. Histological and immunohistochemical analyses revealed the presence of large B cells in lesional tissues, which were occasionally monoclonal. The presence of B-cell clones was highly associated with EBV positivity, as revealed by in situ hybridization. In two cases that were evaluated by serial histological and molecular examination, EBV-positive cells persisted in one and disappeared in the other. These findings suggest a role for EBV in the evolution of B-cell clones in T-cell lymphomas.
- Published
- 2011
99. Epstein-barr virus in diffuse large B-Cell lymphoma in immunocompetent patients in Japan is as low as in Western Countries
- Author
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Naoki Wada, Eiichi Morii, Toshihiro Soma, Shirou Fukuhara, Haruo Sugiyama, Masayuki Hino, Shigeki Fujita, Yuzuru Kanakura, Hiroyasu Ogawa, Akihisa Kanamaru, Katsuyuki Aozasa, Yumiko Hori, and Jun-ichiro Ikeda
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Epstein-Barr Virus Infections ,Herpesvirus 4, Human ,Adolescent ,Biology ,medicine.disease_cause ,Virus ,Herpesviridae ,Japan ,hemic and lymphatic diseases ,Virology ,medicine ,Gammaherpesvirinae ,Humans ,Epstein–Barr virus infection ,Aged ,Aged, 80 and over ,Cancer ,Middle Aged ,medicine.disease ,biology.organism_classification ,Epstein–Barr virus ,Lymphoma ,Infectious Diseases ,Female ,Lymphoma, Large B-Cell, Diffuse ,Diffuse large B-cell lymphoma ,Immunocompetence - Abstract
According to previous reports, the frequency of Epstein-Barr virus (EBV) positivity in diffuse large B-cell lymphoma is higher in East Asia (approximately 9%) than in Western countries. The presence of the EBV genome was examined in diffuse large B-cell lymphoma patients registered with the Osaka Lymphoma Study Group (OLSG) in Osaka, Japan, situated in East Asia. The EBV-positive rate was examined with in situ hybridization (ISH) in 484 immunocompetent diffuse large B-cell lymphoma patients registered with OLSG. The male-to-female ratio was 1.29, with ages ranging from 16 to 95 (median, 68) years. ISH with EBV-encoded small RNAs (EBER) probes revealed positive signals in the nuclei of tumor cells: the frequency of positively stained cells among all tumor cells was almost none in 458 cases, 5-10% in 5, 10-20% in 5, 20-50% in 11, and >50% in 5. When the frequency was >20% or >50%, the EBV-positive rate in the present series (3.3% or 1.0%) was rather similar to that reported in Western cases. Careful evaluation of patient backgrounds, including age distribution, type of lymphomas, exclusion of immunocompromised patients, and establishment of definite criteria for EBV positivity (>20%, >50%, or almost all tumor cells) are essential in comparing geographical differences.
- Published
- 2010
100. Hot water extract of adzuki (Vigna angularis) suppresses antigen-stimulated degranulation in rat basophilic leukemia RBL-2H3 cells and passive cutaneous anaphylaxis reaction in mice
- Author
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Tomohiro, Itoh, Yumiko, Hori, Toshiyuki, Atsumi, Kazuo, Toriizuka, Masahiro, Nakamura, Takeshi, Maeyama, Masashi, Ando, Yasuyuki, Tsukamasa, Yoshiteru, Ida, and Yukio, Furuichi
- Subjects
Male ,Mice, Inbred ICR ,Plant Extracts ,Passive Cutaneous Anaphylaxis ,Fabaceae ,Cell Degranulation ,Basophils ,Rats ,Mice ,Leukemia, Basophilic, Acute ,Cell Line, Tumor ,Animals ,Calcium ,Reactive Oxygen Species ,Signal Transduction - Abstract
The hot water extract of adzuki (HWEA), which is produced as a byproduct in the adzuki bean boiling process, has anti-tumor, antioxidative, and anti-diabetic activities. In this study, we fractionated HWEA to 4 fractions using stepwise gradient column chromatography with water and ethanol, and demonstrated the effects of each fraction on antigen (Ag)-stimulated degranulation in rat basophilic leukemia RBL-2H3 cells. The 40% ethanol eluate extract (EtEx.40) showed the strongest inhibition level of these fractions. To reveal the inhibitory mechanisms underlying degranulation by EtEx.40, we investigated intracellular reactive oxygen species (ROS) production, intracellular free Ca²⁺ concentration ([Ca²⁺]i), and early intracellular signaling pathways. Treatment with EtEx.40 markedly inactivated Lyn following Ag stimulation, resulting in the suppressions of intracellular elevation of [Ca²⁺]i and production of ROS. To identify the active compound in EtEx.40, we isolated 7 flavonoids from EtEx.40 and calculated their inhibition levels on Ag-stimulated degranulation. These flavonoids inhibited degranulation by about 25-60%. We further examined the in vivo effects of HWEA or EtEx.40 using a passive cutaneous anaphylaxis (PCA) reaction. Both extracts strongly suppressed the PCA reaction. These findings suggest that HWEA and/or EtEx.40 are beneficial for alleviating type I allergic symptoms.
- Published
- 2010
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