51. Specific Neuroligin3–αNeurexin1 signaling regulates GABAergic synaptic function in mouse hippocampus
- Author
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Yuka Imamura Kawasawa, Timmy Le, Masahiko Watanabe, Takeshi Uemura, Emily Demchak, Amy Cheung, Kenji Sakimura, David G Keener, Kohtarou Konno, Kensuke Futai, Toshikuni Sasaoka, Manabu Abe, Takuya Watanabe, and Motokazu Uchigashima
- Subjects
0301 basic medicine ,Gene isoform ,inhibitory interneuron ,Male ,Mouse ,QH301-705.5 ,hippocampus ,Science ,Cell Adhesion Molecules, Neuronal ,Hippocampus ,Nerve Tissue Proteins ,Neurotransmission ,Inhibitory postsynaptic potential ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Postsynaptic potential ,Animals ,Biology (General) ,GABAergic Neurons ,CA1 Region, Hippocampal ,Neural Cell Adhesion Molecules ,General Immunology and Microbiology ,Cell adhesion molecule ,Chemistry ,General Neuroscience ,Calcium-Binding Proteins ,Membrane Proteins ,General Medicine ,electrophysiology ,trans-synaptic adhesion ,Cell biology ,Mice, Inbred C57BL ,030104 developmental biology ,Gene Knockdown Techniques ,Synapses ,Excitatory postsynaptic potential ,Medicine ,GABAergic ,Female ,030217 neurology & neurosurgery ,Research Article ,Neuroscience - Abstract
Synapse formation and regulation require signaling interactions between pre- and postsynaptic proteins, notably cell adhesion molecules (CAMs). It has been proposed that the functions of neuroligins (Nlgns), postsynaptic CAMs, rely on the formation of trans-synaptic complexes with neurexins (Nrxns), presynaptic CAMs. Nlgn3 is a unique Nlgn isoform that localizes at both excitatory and inhibitory synapses. However, Nlgn3 function mediated via Nrxn interactions is unknown. Here we demonstrate that Nlgn3 localizes at postsynaptic sites apposing vesicular glutamate transporter 3-expressing (VGT3+) inhibitory terminals and regulates VGT3+ inhibitory interneuron-mediated synaptic transmission in mouse organotypic slice cultures. Gene expression analysis of interneurons revealed that the αNrxn1+AS4 splice isoform is highly expressed in VGT3+ interneurons as compared with other interneurons. Most importantly, postsynaptic Nlgn3 requires presynaptic αNrxn1+AS4 expressed in VGT3+ interneurons to regulate inhibitory synaptic transmission. Our results indicate that specific Nlgn–Nrxn signaling generates distinct functional properties at synapses.
- Published
- 2020