51. Antiarrhythmic effects and potential mechanism of WenXin KeLi in cardiac Purkinje cells
- Author
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Chang-Yi Li, Xiao-Meng Chen, Jing Zhao, Yi-Gang Li, Yi-He Chen, Bu-Chang Zhao, Hong Wang, Wei Li, Yue-Peng Wang, Kai Guo, and Jian-Wen Hou
- Subjects
0301 basic medicine ,Patch-Clamp Techniques ,chemistry.chemical_element ,Action Potentials ,030204 cardiovascular system & hematology ,Pharmacology ,Calcium ,Sodium current ,03 medical and health sciences ,Purkinje Cells ,0302 clinical medicine ,Heart Conduction System ,Physiology (medical) ,medicine ,Animals ,Wenxin keli ,Myocytes, Cardiac ,Patch clamp ,Potential mechanism ,Flecainide ,business.industry ,Arrhythmias, Cardiac ,Nap ,Electrophysiology ,030104 developmental biology ,chemistry ,Rabbits ,Cardiology and Cardiovascular Medicine ,business ,medicine.drug ,Drugs, Chinese Herbal - Abstract
Background Previous studies have demonstrated that WenXin KeLi (WXKL), a traditional Chinese medicine, can exert antiarrhythmic properties through complex multichannel inhibition, but its pharmacologic effect remains to be elucidated, especially in the cardiac conductive system. Objective To explore the antiarrhythmic property of WXKL in cardiac Purkinje cells (PCs). Methods PCs were isolated from rabbit hearts and action potentials (APs) and ion currents were recorded by whole-cell patch clamp technique. Anemonia toxin II (ATX-II) and isoproterenol (ISO) were used to induce early or delayed afterdepolarizations (EADs, DADs) or triggered activities (TAs). Results WXKL (1 g/L and 5 g/L) significantly abbreviated the action potential duration (APD) of PCs in a dose- and rate-dependent manner. Treatment of PCs with ATX-II (2 nM) prolonged APD and induced EADs, which were significantly suppressed by WXKL. WXKL (1, 5 g/L) also inhibited ISO-induced EADs, DADs, and TAs. To reveal the ionic mechanisms, we studied the effects of WXKL on late sodium current (I NaL ), peak sodium current (I NaP ), and L-type calcium currents (I CaL ) in PCs. WXKL-attenuated ATX-II (5 nM) induced I NaL augmentation and blocked I NaL with an IC 50 of 4.3 ± 0.5 g/L, which is 3- to 4-fold more selective than that of I NaP (13.3 ± 0.9 g/L) and I CaL (17.6 ± 1.4 g/L). Moreover, WXKL exerted significantly less use-dependent block of I NaP than that of flecainide, indicating its lower proarrhythmic effect. Conclusions WXKL exhibits antiarrhythmic properties in cardiac PCs via selective inhibition of I NaL .
- Published
- 2015