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Objectives: To identify the optimal screw configuration for internal fixation of femoral neck fractures based on anatomic analysis on radiologic imaging. Methods: 30 proximal femurs of 15 adults were constructed by CT. 3 femoral neck sections (FNS), the subcapital, medial, and the fundus, were projected on to the lateral femoral trochanteric wall. The simulated 3 screw configurations in the projection of FNS include: 2 inverted equilateral triangles symmetrised to the axis of the FNS (IET-FNS group) or the coronal axis of the proximal femur (IET-PR group) and an obtuse triangle (OT group). The distance between the screws, the distance between the centre of the FNS and the screws, and the area ratio of the triangle/FNS were calculated. Results: The projection of the FNS on to the lateral femoral trochanteric wall is displayed as a rotating forward ellipse. Measurements of distance between screws, distance between the centre of the FNS to the screws, and the area ratio of triangle/FNS were significantly larger in the OT group than in the IET-FNS and IET-PF groups (p < 0.05). The values of the 3 parameters in the IET-FNS group were also larger than those in the IET-PF group (p < 0.05). Conclusions: The obtuse triangle screw configuration displayed advantages with respect to the parameters of distance between screws, distance between the centre of FNS to screws, and the triangle area. Therefore, the obtuse triangle screw configuration may be the ideal pattern for internal fixation of femoral neck fractures (Pauwels I and II). This needs to be corroborated with biomechanics testing. [ABSTRACT FROM AUTHOR]

In this presentation, extra-large size and high resolution ADS panels have been developed successfully and described on panel processes and architectures, as are 110inch UHD 120Hz and QUHD, 98 inch QUHD, 82 inch 10K4K (Aspect ratio 21:9) and 65 inch QUHD. Several important improvements on ADS TV panel were introduced, such as low color shift at dark state and low storage capacitance. [ABSTRACT FROM AUTHOR]

When the ratio of W/S in ADS mode LCD is tuned, V-T Curve symmetry is altered by splay deformations in areas above the pixel electrode and slit, and flicker shift and DC image sticking are changed by bend deformations in area above the border of the pixel electrode and slit. [ABSTRACT FROM AUTHOR]

Background: In recent decades, natural compounds have been considered a significant source of new antitumor medicines due to their unique advantages. Several in vitro and in vivo studies have focused on the effect of terpenoids on apoptosis mediated by mitochondria in malignant cells., Recent Findings: In this review article, we focused on six extensively studied terpenoids, including sesquiterpenes (dihydroartemisinin and parthenolide), diterpenes (oridonin and triptolide), and triterpenes (betulinic acid and oleanolic acid), and their efficacy in targeting mitochondria to induce cell death. Terpenoid-induced mitochondria-related cell death includes apoptosis, pyroptosis, necroptosis, ferroptosis, autophagy, and necrosis caused by mitochondrial permeability transition. Apoptosis and autophagy interact in meaningful ways. In addition, in view of several disadvantages of terpenoids, such as low stability and bioavailability, advances in research on combination chemotherapy and chemical modification were surveyed., Conclusion: This article deepens our understanding of the association between terpenoids and mitochondrial cell death, presenting a hypothetical basis for the use of terpenoids in anticancer management., (© 2024 The Author(s). Cancer Reports published by Wiley Periodicals LLC.)

Transketolase (EC 2.2.1.1, TK) is a thiamine diphosphate-dependent enzyme that catalyzes the transfer of a two-carbon hydroxyacetyl unit with reversible C-C bond cleavage and formation. It is widely used in the production of chemicals, drug precursors, and asymmetric synthesis by cascade enzyme catalysis. In this paper, the activity of transketolase TKTA from Escherichia coli K12 on non-phosphorylated substrates was enhanced through site-directed saturation mutation and combined mutation. On this basis, the synthesis of tartaric semialdehyde was explored. The results showed that the optimal reaction temperature and pH of TKTA&#95;M (R358I/H461S/R520Q) were 32 ℃ and 7.0, respectively. The specific activity on d-glyceraldehyde was (6.57±0.14) U/mg, which was 9.25 times higher than that of the wild type ((0.71±0.02) U/mg). Based on the characterization of TKTA&#95;M, tartaric acid semialdehyde was synthesized with 50 mmol/L 5-keto-d-gluconate and 50 mmol/L non-phosphorylated ethanolaldehyde. The final yield of tartaric acid semialdehyde was 3.71 g with a molar conversion rate of 55.34%. Hence, the results may facilitate the preparation of l-(+)-tartaric acid from biomass, and provide an example for transketolase-catalyzed non-phosphorylated substrates.

Pyrroloquinoline quinone (PQQ), an important redox enzyme cofactor, has many physiological and biochemical functions, and is widely used in food, medicine, health and agriculture industry. In this study, PQQ production by recombinant Gluconobacter oxydans was investigated. First, to reduce the by-product of acetic acid, the recombinant strain G. oxydans T1 was constructed, in which the pyruvate decarboxylase (GOX1081) was knocked out. Then the pqqABCDE gene cluster and tldD gene were fused under the control of endogenous constitutive promoter P0169, to generate the recombinant strain G. oxydans T2. Finally, the medium composition and fermentation conditions were optimized. The biomass of G. oxydans T1 and G. oxydans T2 were increased by 43.02% and 38.76% respectively, and the PQQ production was 4.82 and 20.5 times higher than that of the wild strain, respectively. Furthermore, the carbon sources and culture conditions of G. oxydans T2 were optimized, resulting in a final PQQ yield of (51.32±0.899 7 mg/L), 345.6 times higher than that of the wild strain. In all, the biomass of G. oxydans and the yield of PQQ can be effectively increased by genetic engineering.

The current Shigella sonnei pandemic involves geographically associated, multidrug-resistant clones. This study has demonstrated that S. sonnei phylogeny can be accurately defined with limited single nucleotide polymorphisms (SNPs). By typing 6 informative SNPs using a high-resolution melting (HRM) assay, major S. sonnei lineages/sublineages can be identified as defined by whole-genome variation.