51. Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish
- Author
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Hiroko Nakayama, Shu-Ping Hui, Yusuke Miura, Yasuhito Shimada, Zhen Chen, Hitoshi Chiba, Rojeet Shrestha, Yue Wu, Yaoyao Zhao, Liqing Zang, and Norihiro Nishimura
- Subjects
0301 basic medicine ,Antioxidant ,type 2 diabetes mellitus ,medicine.medical_treatment ,Phospholipid ,medicine.disease_cause ,01 natural sciences ,Analytical Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Lipid oxidation ,Diabetes mellitus ,medicine ,Animals ,Metabolomics ,Zebrafish ,Phospholipids ,biology ,Chemistry ,010401 analytical chemistry ,oxidative stress biomarkers ,Type 2 Diabetes Mellitus ,medicine.disease ,biology.organism_classification ,zebrafish ,0104 chemical sciences ,LC-MS ,Disease Models, Animal ,030104 developmental biology ,Lysophosphatidylcholine ,Diabetes Mellitus, Type 2 ,Biochemistry ,lipids (amino acids, peptides, and proteins) ,oxidized phospholipids ,Oxidation-Reduction ,Biomarkers ,Oxidative stress - Abstract
Type 2 diabetes mellitus is a serious metabolic disorder in the world. Oxidative stress, as a key role on the pathogenesis of diabetes, also results in the oxidation of phospholipids. However, studies on phospholipid oxidation in diabetes, especially directly focusing on oxidized and degraded phospholipid species, are quite limited. In this study, phospholipid profiles of diabetic zebrafish plasma were characterized by LC-HRMS and MS/MS, and the total amounts of each lipid class were compared. Furthermore, the key molecular species as biomarkers in distinguishing control and diabetic samples were investigated by orthogonal partial least squares discriminant analysis. Among the identified 114 phospholipid species in total, there were 11 hydroperoxides, 7 aldehydes, and 19 lysophospholipids found significantly elevated along with the increasing blood glucose, which were known as oxidation or degradation products. Furthermore, lysophosphatidylcholine 20:5 and lysophosphatidylcholine 22:6 were assessed as potential biomarkers in diabetic zebrafish. The current work would not only help to gain further insights into diabetes, but also contribute to find new clinical parameters for the screening of the promising antioxidant agents for its therapies.
- Published
- 2018