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Purpose – The purpose of this paper is to focus on testing hypotheses derived from theories presented by leading scholars regarding possible sources of differences in reliability coefficients, using cross-sectional data from Japan and Malaysia. Design/methodology/approach – A survey was conducted in Malaysia and Japan, both of them characterized as having collectivist values. Convenience sampling and the same method of survey administration, personal interviews by trained interviewers, was used in both countries. Findings – The tests of the hypotheses regarding cultural, subcultural, and aging effects provide some results in line with the hypotheses, but they raise additional concerns about the possible sources of measurement differences and the applicability of theories and measures developed in one country to other countries and subcultures within countries. The major findings include the alpha reliability coefficient of the self-esteem scale (SSE) which is hypothesised to be lower than the alpha for the material value scale (MVS) and satisfaction with life scale (SWLS) in the Malaysian and Japanese samples, but this difference only occurs for the Japanese sample. Research limitations/implications – The study findings highlight the challenges that researchers confront when conducting cross-cultural studies. Originality/value – The present study systematically tests the reliability of measures developed in the West and employed in different countries (especially Asian countries). Although several assumptions and speculations have been made regarding the sources of potential bias in measures used when studying cultural and subcultural differences, little empirical work supports these views. Our study fills this gap. [ABSTRACT FROM AUTHOR]

Hydrocarbon contamination introduced during point, line and map analyses in a field emission electron probe microanalysis (FE-EPMA) was investigated to enable reliable quantitative analysis of trace amounts of carbon in steels. The increment of contamination on pure iron in point analysis is proportional to the number of iterations of beam irradiation, but not to the accumulated irradiation time. A combination of a longer dwell time and single measurement with a liquid nitrogen (LN2) trap as an anti-contamination device (ACD) is sufficient for a quantitative point analysis. However, in line and map analyses, contamination increases with irradiation time in addition to the number of iterations, even though the LN2 trap and a plasma cleaner are used as ACDs. Thus, a shorter dwell time and single measurement are preferred for line and map analyses, although it is difficult to eliminate the influence of contamination. While ring-like contamination around the irradiation point grows during electron-beam irradiation, contamination at the irradiation point increases during blanking time after irradiation. This can explain the increment of contamination in iterative point analysis as well as in line and map analyses. Among the ACDs, which are tested in this study, specimen heating at 373 K has a significant contamination inhibition effect. This technique makes it possible to obtain line and map analysis data with minimum influence of contamination. The above-mentioned FE-EPMA data are presented and discussed in terms of the contamination-formation mechanisms and the preferable experimental conditions for the quantification of trace carbon in steels., (© The Author 2016. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Tear lipocalin and beta-lactoglobulin are members of the lipocalin superfamily. They have similar tertiary structures but unusually low overall sequence similarity. Non-native helical structures are formed during the early stage of beta-lactoglobulin folding. To address whether the non-native helix formation is found in the folding of other lipocalin superfamily proteins, the folding kinetics of a tear lipocalin variant were investigated by stopped-flow methods measuring the time-dependent changes in circular dichroism (CD) spectrum and small-angle X-ray scattering (SAXS). CD spectrum showed that extensive secondary structures are not formed during a burst-phase (within a measurement dead time). The SAXS data showed that the radius of gyration becomes much smaller than in the unfolded state during the burst-phase, indicating that the molecule is collapsed during an early stage of folding. Therefore, non-native helix formation is not general for folding of all lipocalin family members. The non-native helix content in the burst-phase folding appears to depend on helical propensities of the amino acid sequence.