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鼻出血是耳鼻咽喉科的常见急症之一，但鼻出血伴发急性期脑出血的高危情况较为少见。本文总结分析了2例就诊于北京朝阳医院的伴有急性期脑出血的鼻出血患者的病例资料，包括临床表现、实验室检查、影像学检查及治疗情况等。并结合国内外相关文献，总结了鼻出血患者并发急性脑出血的特点，提示耳鼻咽喉科医生对于有脑出血高风险的鼻出血患者做到早发现、早干预，避免延误治疗。.

Three off-shore islands of Taiwan: Penghu, Kinmen and Matsu are critical in tactical position for Taiwan military, but lack of scarcity resource. Therefore, logistics is very important especially for the off-shore islands. Practically, most of the goods and materials are transported to the islands in the way of shipping and air transportation by Taiwan. However, the severe weather of the islands makes the transportation difficult or even to delay, which is a serious problem for the military logistics of the islands. To raise transportation performance, a proper evaluation method is necessary for military logistics to select the best transport mode based on reducing cost and emphasizing efficiency. Generally, analytic hierarchy process (AHP) may be a method in the selection of transport modes for Taiwan off-shore islands’ military logistics. However, some computation procedures in AHP are hard or complicated especially for processing numerous interviewees’ messages. To resolve the above tie, we utilize fuzzy analytic hierarchy process(FAHP) in selecting transport modes for Taiwan off-shore islands’ military logistics in this paper. After integrating interviewees’(i.e., querying soldiers’) opinions into fuzzy pair-wise comparison matrices, FAHP, being different from AHP, simply and efficiently yields priorities of the fuzzy pair-wise comparison matrices to find the best transport mode of Taiwan off-shore islands’ military logistics.

Acoustophoretic assembly uses acoustic waves to move dispersed particles into a geometric pattern. The pattern is typically created in a single step and often relies on wave-forming techniques to achieve the desired pattern geometries. We show that multiple acoustic waves can be applied sequentially in a multi-step process to create particle patterns not achievable by the individual waves alone. We demonstrate this approach in spherical particles using two planar pseudo-standing waves. Applied individually, each of the two waves would create linear particle bands with uniform spacing in between the bands. However, when applied sequentially, the banding pattern created in the first step is further manipulated by the second wave to create non-uniform spacing in between the bands. The experimentally achieved particle pattern geometry agrees well with the theoretical prediction.

Regarding to the impact of temperature on springback in hot stretch bending (HSB) process, this work proposed an investigation on temperature uniformity control of titanium extrusion in HSB process based on the mould which can be elevated by resistance heating. Prior to the HSB test, mould structure was designed to fit for resistance heating. Coupled thermal electrical structural finite element model of HSB process was created in ABAQUS. Resistance heating and HSB tests were performed using displacement control electrically assisted stretch bending machine. The results of simulation indicate that the temperature uniformity of titanium extrusion and the forming accuracy of curved part can be effectively improved by increasing the initial temperature of mould. At last, the curved part of titanium extrusion with L-section was achieved, and the forming accuracy of which was measured and evaluated.

In amorphous solids, plastic flow is prone to localization into shear bands via an avalanche of shear-transformation (ST) rearrangements of constituent atoms or particles. However, such banding instability still remains a lack of direct experimental evidence. Using a real 3D colloidal glass under shear as proof of principle, we study STs' avalanches into shear banding that is controlled by strain rates. We demonstrate that, accompanying the emergent shear banding, the elastic response fields of the system, typical of a quadrupole for shear and a centrosymmetry for dilatation, lose the Eshelby-type spatial symmetry; instead, a strong correlation appears preferentially along the banding direction. By quantifying the fields' spatial decay, we identify an elastic criterion for the shear-banding instability, that is, the strongly correlated length of dilatation is smaller than the full length of shear correlation. Specifically, ST-induced free volume has to be confined within the elastic shear domain of ST so that those STs can self-organize to trigger shear banding. This physical picture is directly visualized by tracing the real-space evolution of local dilatation and ST particles. The present work unites the two classical mechanisms: free volume and STs, for the fundamental understanding of shear banding in amorphous solids.

Nanometer-thick passive films, which impart superior corrosion resistance to metals, are degraded in long-term service; they are also susceptible to chloride-induced localized attack. Here we show, by engineering crystallographic configurations upon metal matrices adjacent to their passive films, we obtain great enhancement of corrosion resistance of FeCr15Ni15 single crystal in sulphuric acid, with activation time up to two orders of magnitude longer than that of the non-engineered counterparts. Meanwhile, engineering crystallography decreases the passive current density and shifts the pitting potential to noble values. Applying anodic polarizations under a transpassivation potential, we make the metal matrices underneath the transpassive films highly uneven with {111}-terminated configurations, which is responsible for the enhancement of corrosion resistance. The transpassivation strategy also works in the commercial stainless steels where both grain interior and grain boundaries are rebuilt into the low-energy configurations. Our results demonstrate a technological implication in the pretreatment process of anti-corrosion engineering.

川崎病是一种急性、自限性发热性疾病，病因不明，在急性发热期主要影响中型动脉、多个脏器和组织。最新研究发现白细胞介素（IL）-1信号通路是川崎病的致病关键环节之一。IL-1受体拮抗剂（IL-1Ra）阿那白滞素通过阻断IL-1信号通路，对于川崎病的治疗有一定的作用，但其应用的临床证据较少，本文就阿那白滞素治疗川崎病的作用机制、疗效及安全性进行综述，旨在为该药治疗川崎病提供依据。.

OBJECTIVE: To assess the mid-to-long term clinical outcomes after anterior cruciate ligament (ACL) revision surgery and to analyze their predictors. METHODS: The medical records of 235 patients undergoing ACL revision surgery between Jan. 2001 and Dec. 2015 at Department of Sports Medicine, Peking University Third Hospital were reviewed. Data were collected including demographic information, information related to revision surgery (time and cause of graft failure, date of revision surgery, surgical technique, combined injuries and management, etc.), as well as information related to primary ACL reconstruction (time, cause and mechanism of first-time ACL rupture, date of primary ACL reconstruction, surgical technique, combined injuries and management, etc.). Patients were followed up at least 2 years after revision surgery for clinical outcomes [Tegner score, Lysholm score, and International Knee Documentation Committee (IKDC) subjective knee score]. Post-revision surgeries on the involved knee and the contralateral knee joint were also documented. Multivariate regression model was used to analyze the predictors of clinical outcomes after ACL revision surgery. RESULTS: A total of 166 (70.63%) patients were followed up at a mean of (4.44±2.40) years (2.03-14.63 years). Clinical outcomes improved significantly at the last follow-up from pre-operative level, with the Lysholm, Tegner, and IKDC scores improving from 70.51±21.25, 3.39±1.77, 63.78±15.04 to 88.64±14.36, 4.67±1.739, 80.23±13.31 (P < 0.05), respectively. Three (1.81%) patients experienced infection while 39 (23.49%) patients underwent surgery after revision surgery during the follow-up. Compared with that those occurred during sports, graft failure that occurred during daily activities or due to surgical technical errors that led to poorer clinical outcomes, with the Lysholm, Tegner, and IKDC scores of 9.90 (95%CI: 1.49-18.31), 1.41 (95%CI: 0.10-2.72), 10.35 (95%CI: 0.17-20.54), and 8.53 (95%CI: 1.31-15.75), 1.28 (95%CI: 0.14-2.43), 9.39 (95%CI: 1.03-17.74) lower, respectively. Compared with antero-medial portal, transtibial technique for placement of the femoral bone tunnel showed poorer Lysholm scores of 11.18 (95%CI: 4.73-17.63, P=0.001). Concurrent repair of medial meniscus yielded higher IKDC scores of 11.06 (95%CI: 1.21-20.92, P=0.029) than those with intact medical meniscus. Other factors showed no significant effect. CONCLUSION: ACL revision surgery is able to restore knee stability and improve knee function. Graft failure caused by sports, concurrent repair of medical meniscus and antero-medial portal technique predicts better outcomes after revision surgery.

To investigate the population dynamics andRodents were captured using the mouse trap method in pastures from Batang Township and Longbao Township of Yushu City, Qinghai Province on May, August and October, 2018. The body weight and snout-vent length of all captured rodents were measured, and the species was identified according to the rodent morphology. Genomic DNA was extracted from rodent liver specimens and lesion specimens, and the mitochondrialA total of 285 small rodents were captured, including 143The small rodents around the human settlement in Yushu City of Qinghai Province mainly include

Recently, the LHAASO Collaboration published the detection of 12 ultrahigh-energy γ-ray sources above 100 TeV, with the highest energy photon reaching 1.4 PeV. The first detection of PeV γ rays from astrophysical sources may provide a very sensitive probe of the effect of the Lorentz invariance violation (LIV), which results in decay of high-energy γ rays in the superluminal scenario and hence a sharp cutoff of the energy spectrum. Two highest energy sources are studied in this work. No signature of the existence of the LIV is found in their energy spectra, and the lower limits on the LIV energy scale are derived. Our results show that the first-order LIV energy scale should be higher than about 10^{5} times the Planck scale M_{Pl} and that the second-order LIV scale is10^{-3}M_{Pl}. Both limits improve by at least one order of magnitude the previous results.

结外NK/T细胞淋巴瘤（ENKTL）是一种与EB病毒密切相关、恶性程度较高的非霍奇金淋巴瘤（NHL），多种因素影响其预后。既有的ENKTL预后模型（国际预后指数、韩国预后指数）对疾病预后判断价值有限，且不能适应当代的非蒽环类药物治疗时代。因此，本文根据国内外研究进展，对EB病毒、分子遗传学和肿瘤微环境相关的预后因素进行综述，以寻求建立更有效的预后模型和发现新的治疗靶点，提高ENKTL患者的生存率。.

The present study aims to understand the mechanisms controlling ecosystem metabolic states and atmospheric CO2 fluxes and explore their linkages through monthly investigations along the tropical Kaoping Submarine Canyon (KPSC) in southwestern Taiwan. The magnitudes of integrated gross production (IGP) and integrated dark community respiration (IDCR) were higher in summer than in winter, likely supported by greater inputs of freshwater in summer with high loads of nutrients and organic carbon. Strong positive correlations were observed between gross production (GP) (or dark community respiration (DCR)) and temperature, photosynthetically active radiation and nutrients, but negative correlations were observed between GP (or DCR) and salinity in discrete water samples; these results reveal the significant impacts of freshwater inputs on GP and DCR. GP was determined largely by DCR, and DCR was attributed largely (78%) to bacterial respiration in the KPSC. Approximately 60% of measurements were autotrophic conditions (IGP/IDCR >1) in most wet seasons except for certain offshore stations, which were apparently driven by higher temperature and nutrient availability, whereas 40% of measurements revealed heterotrophic conditions (IGP/IDCR

Domain behavior of (111)- oriented perovskite ferroelectric films is significantly different from (001)-/(101)- oriented ones, resulting in enhancing property responses such as a superior susceptibility and a reduced coercive field. However, the domain structures and evolutions with the thickness of (111)-oriented ferroelectric films, which are crucial to further understand the distinctive properties, are still obscure. In this study, the ferroelectric domains of (111)- oriented PbTiO3 films are investigated by transmission electronic microscopy (TEM) and piezoresponse force microscopy (PFM). We identify the domain evolution with the film thicknesses under anisotropic strains imposed by the orthorhombic GdScO3 (101)O substrates. Contrast analysis and electron diffraction patterns reveal that only four ferroelectric variants evolve in PTO films: d2+, d2-, d3+ and d3-, with the polarization directions along [010], [0 1 ¯ 0], [001] and [00 1 ¯ ], respectively. Two kinds of domain walls are formed: “inclined” (011) domain walls for d2-/d3+ (d2+/d3-) domains, “normal” (01 1 ¯ ) domain walls for d2-/d3+ (d2+/d3-) domains. The width of periodically distributed d2+/d3+ (d2-/d3-) domains increases with film thickness following the square root rule. Aberration-corrected scanning transmission electronic microscopy demonstrates the lattice characteristics of domains in (111)- oriented PbTiO3 films are consistent with tetragonal ferroelectric domains. PFM studies reveal that both the out-of-plane and in-plane polarization components of d2-/d3+ (d2+/d3-) domains are non-identical, whereas the d2+/d3+ (d2-/d3-) domains possess uniform out-of-plane polarization component and non-uniform in-plane polarization component. This study discloses the domain structure in (111)- oriented tetragonal ferroelectric films under anisotropic strains and the association with the ferroelectric properties.

Lignification of cellulose limits the effective utilisation of fibre in plant cell wall. Lignocellulose-degrading bacteria secrete enzymes that decompose lignin and have the potential to improve fibre digestibility. Therefore, this study aimed to investigate the effect of whole-plant corn silage inoculated with lignocellulose-degrading bacteria on the growth performance, rumen fermentation, and rumen microbiome in sheep. Twelve 2-month-old male hybrid sheep (Dorper ♂ × small-tailed Han ♀) were randomly assigned into two dietary groups (n = 6): (1) untreated whole-plant corn silage (WPCS) and (2) WPCS inoculated with bacterial inoculant (WPCSB). Whole-plant corn silage inoculated with bacterial inoculant had higher in situ NDF digestibility than WPCS. Sheep in the WPCSB group had significantly higher average daily gain, DM intake, and feed conversion rate than those in the WPCS group (P 0.05). Furthermore, higher volatile fatty acid concentrations were detected in WPCSB rumen samples, leading to lower ruminal pH (P 0.05). The WPCSB group showed higher abundance of Bacteroidetes and lower abundance of Firmicutes in the rumen microbiome than the WPCS group (P 0.05). Multiple differential genera were identified, with Prevotella being the most dominant genus and more abundant in WPCSB samples. Moreover, the enriched functional attributes, including those associated with glycolysis/gluconeogenesis and citrate cycle, were more actively expressed in the WPCSB samples than in the WPCS samples. Additionally, certain glucoside hydrolases that hydrolyse the side chains of hemicelluloses and pectins were also actively expressed in the WPCSB microbiome. These findings suggested that WPCSB increased NDF digestibility in three ways: (1) by increasing the relative abundance of the most abundant genera, (2) by recruiting more functional features involved in glycolysis/gluconeogenesis and citrate cycle pathways, and (3) by increasing the relative abundance and/or expression activity of the glucoside hydrolases involved in hemicellulose and pectin metabolism. Our findings provide novel insights into the microbial mechanisms underlying improvement in the growth performance of sheep/ruminants. However, the biological mechanisms cannot be fully elucidated using only metagenomics tools; therefore, a combined multi-omics approach will be used in subsequent studies.

Polar topological domain structures may have potential applications in future memory devices. Exploring their dynamical behaviors is paramount to the device design. Here, the meron–antimeron annihilation processes induced by the electric field in ultrathin ferroelectric PbTiO3 films were studied by phase-field simulations. Two types of annihilation behaviors were identified: The merons with central polarizations parallel to the electric field will directly annihilate with their nearby antimerons; those antiparallel to the electric field will first be switched and then annihilate with antimerons. The annihilation ratio of meron–antimeron pairs (MAPs) decreases with the increase in the meron–antimeron distance. During annihilation processes, the positions of antimerons seldom change and the merons approach to antimerons at some critical electric fields. The annihilation probability of a meron is found to be closely related to its topological charge. Specifically, the restoration of an MAP from a partially annihilated state was observed under the decrease in the electric field. These results may have some indications to the development of polar-meron-based memory devices.

Eight dissociation constants of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis (methylene phosphonic acid) (DOTMP, H8L) were determined for the first time by potentiometric titration in water using the CHEMEQUI program. The stability constants of Ga3+ complexes with deprotonated forms of DOTMP were determined. The stability constant of the Ga3+ complex with the fully deprotonated DOTMP ligand, lg KML equal to 27.8, is higher than the corresponding constant for the Ga3+ complex with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA), the ligand most widely used in radiopharmacy; it is also higher than the constant for the Ga3+ complex with plasma protein transferrin, which makes the DOTMP ligand very promising for the use in radiopharmacy.

RATIONALE: The attempt at direct liberation from mechanical ventilation (MV) using open-circuit oxygen in the tracheostomized patients with prolonged MV might carry a substantial failure rate. Frequent switching between closed-circuit MV support and open-circuit oxygen, such as a T-piece during the liberation attempt, might also carry a risk of dispersing aerosol particles to the care environment, especially during the pandemic era. We aimed to analyze the feasibility and predictability of adding a screening test with automated tube compensation (ATC) or airway resistance compensation (ARC) mode before the attempt of an open-circuit oxygen trial. METHODS: We conducted a retrospective analysis of tracheostomized patients admitted to a medical center's dedicated weaning unit in Taiwan. Because of coronavirus's global pandemic, the unit implemented a universal process to apply ATC/ARC screening tests to patients since April 2020. Before starting the MV liberation trial with continuous spontaneous breathing via open-circuit oxygen (T-piece), the patients received a screening test consisting of 12 hours of ATC/ARC the first day followed by 72 hours of continuous ATC/ARC. Those who passed the test then proceeded to the open-circuit liberation trial. Those who failed the test would receive repeated ATC/ARC or open circuit trials. RESULTS: A total of 79 (51% of the admitted) patients, including 54 male, aged 69 [18-95] years, and averaged 32 days of MV before admission to the weaning unit, received the screening test (59 for ATC;20 for ARC) after MV settings reduction. Of the 79 patients, 48 (61%) succeeded in the test. Upon discharge from the unit, 49 (62%) were liberated from MV, and 43 (54%) were MV-free status 30 days after the ATC/ARC test. Of the 31 who failed ATC/ARC, 22 (71%) remained MV-dependent at 30 days after the ATC/ARC test. Therefore, the sensitivity, specificity, positive predictive value, and negative predictive values of the ATC/ARC test for outcomes of MV-liberation upon discharge from the unit were 90%, 87%, 92%, and 84%, respectively. The sensitivity, specificity, positive predictive value, and negative predictive values of the ATC/ARC test for outcomes of MV-free status 30 days after the test were 93%, 78%, 83%, and 90%, respectively. CONCLUSION: Before proceeding with open-circuit oxygen, a screening test with ATC/ARC provides an optimal prediction for the 30-day outcomes of MV liberation.

静脉溶栓治疗是缺血性卒中发病后再灌注治疗的重要措施，重组组织型纤溶酶原激活剂（recombinant tissue plasminogen activator，r-tPA）是证据最多，应用最为广泛是静脉溶栓药物。筛选出适宜r-tPA静脉溶栓的患者是静脉溶栓成功的关键。本文从静脉溶栓时间窗、年龄、轻型卒中、合并多种疾病或并发多发情况、近期穿刺/手术/外伤、既往服用抗栓药物等多个方面，探讨如何筛选出r-tPA静脉溶栓适宜人群。.

Based on the nonequilibrium Green's function formalism, the spinor Boltzmann equation beyond gradient approximation is derived in a ferromagnetic metal with a single domain wall (DW). We further obtain the charge continuity equation and the spin diffusion equation by integrating over the momentum. By using the spin diffusion equation, we get a generalized spin transfer torque (STT), in which the usual STT is extended to the case beyond the gradient approximation and with inhomogeneous current. We also calculate numerically the physical observables such as charge density $n(x)$, spin accumulation $\mathbit{m}(x)$, current density $j(x)$, spin current density ${\mathbit{j}}_{s}(x)$, etc., by the use of the spinor distribution function. Along with the Landau-Lifshitz-Gilbert-Slonczewski equation that contains the above generalized STT, we can study the motion domain wall, and the critical electric field at an initial velocity of the DW is obtained in terms of the linear stability analysis method.

Coronaviruses are a common class of respiratory viruses that can cause human infections. 2019 novel coronavirus(2019-nCoV), a new coronavirus that has recently caused a pandemic, has affected millions of people and put tremendous pressure on the health systems of almost every country in the world. Coronaviruses are known to spread from person to person through droplets or contact. The 2019-nCoV has also been found in the conjunctival secretions and tears of some clinically diagnosed patients. To assess whether the eye is one of the transmission routes of the virus, we review literature, and summarize the anatomy of the eye-nose pathway, the expression of the virus receptor in the eye, the preclinical animal studies, and the clinical data. We analyze the possibility of eyes as a means of transmission and propose some suggestions of ocular protection.冠状病毒是可感染人类的一类常见呼吸道病毒，已知其是通过飞沫和密切接触在人与人之间传播。近期在世界范围内流行的2019新型冠状病毒（2019-nCoV）已造成数以千万人感染，在部分临床确诊患者的结膜分泌物和泪液中发现了2019-nCoV。本文就冠状病毒在眼表传染的解剖基础、其受体在眼部的表达特征、临床前动物研究结果以及患者临床表现，对文献进行总结和分析，以探讨眼部作为传播途径的可能性，同时提出眼部防护的思考和建议，以供眼科医师在临床工作中参考。

1岁11月龄患儿以腹胀、呕吐起病，临床表现为腹腔高压综合征、重度营养不良，腹部影像学检查提示肠梗阻，剖腹探查见所有肠道严重扩张，未见机械性梗阻，回肠造瘘术后仍有腹胀，后全外显子组基因检测提示为ACTG2基因c.769CT； p.Arg257Cys突变，诊断为原发性内脏肌病致假性肠梗阻。该病临床罕见，预后差，目前以对症支持治疗为主。当患儿出现顽固性难治性腹胀且排除机械性肠梗阻时，需考虑此病，早期基因检测可明确诊断。.

2020年7月

We report the discovery of an extended very-high-energy (VHE) gamma-ray source around the location of the middle-aged (207.8 kyr) pulsar PSR J0622+3749 with the Large High-Altitude Air Shower Observatory (LHAASO). The source is detected with a significance of 8.2σ for E25 TeV assuming a Gaussian template. The best-fit location is (right ascension, declination) =(95.47°±0.11°,37.92°±0.09°), and the extension is 0.40°±0.07°. The energy spectrum can be described by a power-law spectrum with an index of -2.92±0.17_{stat}±0.02_{sys}. No clear extended multiwavelength counterpart of the LHAASO source has been found from the radio to sub-TeV bands. The LHAASO observations are consistent with the scenario that VHE electrons escaped from the pulsar, diffused in the interstellar medium, and scattered the interstellar radiation field. If interpreted as the pulsar halo scenario, the diffusion coefficient, inferred for electrons with median energies of ∼160 TeV, is consistent with those obtained from the extended halos around Geminga and Monogem and much smaller than that derived from cosmic ray secondaries. The LHAASO discovery of this source thus likely enriches the class of so-called pulsar halos and confirms that high-energy particles generally diffuse very slowly in the disturbed medium around pulsars.

We report the discovery of a UHE gamma-ray source, LHAASO J2108+5157, by analyzing the LHAASO-KM2A data of 308.33 live days. Significant excess of gamma-ray induced showers is observed in both energy bands of 25-100 TeV and $\gt$100 TeV with 9.5 sigma and 8.5 sigma, respectively. This source is not significantly favored as an extensive source with the angular extension smaller than the point-spread function of KM2A. The measured energy spectrum from 20 to 200 TeV can be approximately described by a power-law function with an index of -2.83$\pm$ 0.18stat. A harder spectrum is demanded at lower energies considering the flux upper limit set by Fermi-LAT observations. The position of the gamma-ray emission is correlated with a giant molecular cloud, which favors a hadronic origin. No obvious counterparts have been found, deeper multiwavelength observations will help to shed new light on this intriguing UHE source., Comment: 12 pages, 7 figures, accepted by ApJL

We aimed to study the correlations of angiotensin II receptor type 1 (AGTR1) gene polymorphisms with the occurrence and development of eclampsia.A total of 200 pregnant women with eclampsia admitted to our hospital from January 1, 2017 to September 30, 2019 were collected as observation group and 200 normal pregnant women during the same period were recruited in the control group. Genome sequencing was performed to detect the AGTR1 gene polymorphisms in the two groups. Expression level of AGTR1 in both groups was detected. The influences of AGTR1 on clinical data of pregnant women with eclampsia were analyzed.There were no significant differences in age (p=0.545), height (p=0.738), weight (p=0.695) and hypertension (p=0.372) between observation group and control group. However, significant differences were found in the distributions of alleles at AGTR1 rs1799870 (p=0.002) and AGTR1 rs52936049 (p=0.047) between groups. The frequencies of T allele at rs1799870 and rs52936044 in observation group were higher than those in the control group. In addition, the distributions of AGTR1 gene genotypes at rs1799870 (p=0.012), rs144520513 (p=0.008) and rs529360494 (p p =0.036) in observation group differed from those in control group. Observation group had higher frequencies of TT genotype at rs1799870, GG genotype at rs144520513 and TG genotype at rs529360494 than those in control group. Besides, the frequency of CGG haplotype (p=0.008) of AGTR1 gene in observation group was notably lower than that in the control group, while the frequency of TGT haplotype (p=0.012) of AGTR1 gene in the former was remarkably higher than that in the latter. Moreover, the linkage disequilibrium between rs529360494 and rs144520513 of AGTR1 gene was relatively high (D'=0.623). AGTR1 gene polymorphism rs529360494 showed an evident relationship with the expression of AGTR1 gene, and the expression of AGTR1 in pregnant women with eclampsia who carried TG genotype was markedly reduced (p0.05). Furthermore, AGTR1 gene polymorphism rs1799870 was associated with prothrombin time (PT) in pregnant women with eclampsia (p=0.046), and PT in those carrying genotype TC was shorter. Rs144520513 was related to the levels of triglyceride (TG) (p0.001) and low-density lipoprotein (LDL) (p0.001) in pregnant women with eclampsia, and TG and LDL levels were significantly lower.AGTR1 gene polymorphisms are closely associated with the onset and progression of eclampsia.

When expressing streptavidin recombinant polypeptide on magnetosomes (called bacterial magnetic nanoparticles, or BMPs), the presence of endogenous bacterial biotin might be detrimental. In the study, the streptavidin monomer fragment (S1-116) was fused with the intein N-terminal (termed precursor S1-116-IN), and S1-116-IN was expressed in E. coli (BL21). Meanwhile, the SA117-160 fragment was fused with the C-terminal intein, and then this chimeric polypeptide was expressed on magnetosomes by fusion with magnetosome membrance protein MamF. In the in vitro protein splicing system, the purified engineered magnetosomes (BMP-SA117-160-IC) and the S1-116-IN precursor were mixed. Intein-mediated trans-splicing reaction was induced to produce the functional magnetic beads BMP-SA. Our results indicate that intein-mediated protein trans-splicing may lead to efficient synthesis of the recombinant streptavidin on the magnetosomes, showing its promising potential to produce other functional magnetic nanoparticles.

Long non-coding RNA (lncRNA) maternally expressed 3 (MEG3) has been identified to participate in the progression of malignant tumors. However, the role and function of MEG3 in Wilms' tumor (WT) remain unknown. Therefore, the aim of this study was to detect the role of MEG3 in the development of Wilms' tumor, and to explore the underlying mechanism.Expression of MEG3 in WT tissues and blood samples were detected using quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between MEG3 level and clinicopathological character and histogenesis was analyzed. WT-CLS1 and WiT49 cells were cultured in vitro, and the influence of ectopic MEG3 expression was determined. Colony formation assay and Edu assay were employed to measure cell proliferation, while transwell assay and Matrigel assay were adopted to detect cell metastasis. Furthermore, Western blot was applied to explore the mechanism of MEG3 in WT.MEG3 was lowly expressed in WT tissues and blood samples (p0.05). Over-expression of MEG3 significantly reduced the proliferation, invasion and migration of CLS1cells than control cells (p0.05). However, inhibition of MEG3 in WiT49 cells significantly promoted cell growth and metastasis compared with cells in negative control group (p0.05). In addition, MEG3 influenced the protein expression of β-catenin by regulating the Wnt/β-catenin pathway.MEG3 was low-expressed in WT tissues and blood samples. Meanwhile, it could inhibit the proliferation and metastasis of WT cells via wt/β-catenin pathways. All our findings indicated that MEG3 served as a potential target for the diagnosis, treatment and prognosis prediction of WT.

Acute liver injury (ALI) is associated with the Kupffer cells (KCs) inflammation and hepatocytes apoptosis. Previous studies have shown that miR-640 is a valid regulator of the Low-density lipoprotein receptor-related protein 1 (LRP 1) which expressed much lower in an inflammatory condition. However, it is unclear whether MiR-640 inhibition protects against ALI by the up-regulation of LRP 1. To explore the regulated mechanism of miR-640 on acute liver injury.We analyzed the expression of miR-640 in different times of acute injured liver tissues. Lipopolysaccharide (LPS) was employed in provoking the KCs inflammation to injure liver. We used miR-640 mimic or inhibitor to improve or resist the function of miR-640 to explore miR-640 function to ALI via the target of LRP1.We showed that the expression of miR-640 markedly increased in LPS-induced acute injured liver tissues. LPS promoted the progress of ALI, and the inhibition of miR-640 could reverse the injured effects of LPS. Moreover, WNT signaling pathway and LRP1 were significantly enhanced by miR-640 inhibition.These results suggested that miR-640 promotes KCs inflammation via restraining LRP 1 and WNT signaling pathway. But inhibiting miR-640 prevents inflammation damage and ameliorates ALI. MiR-640 inhibition may become a novel target for the therapy of ALI in the future.