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419 results on '"Wolfe, N"'

57. Healthy hunting in central Africa

Author

LeBreton, M., primary, Prosser, A. T., additional, Tamoufe, U., additional, Sateren, W., additional, Mpoudi-Ngole, E., additional, Diffo, J. L. D., additional, Burke, D. S., additional, and Wolfe, N. D., additional

Published
2006
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66. Degradation of Munitions and Chlorinated Solvents by Aquatic Plants

Author

ENVIRONMENTAL PROTECTION AGENCY ATHENS GA ENVIRONMENTAL RESEARCH LAB, Wolfe, N. L., ENVIRONMENTAL PROTECTION AGENCY ATHENS GA ENVIRONMENTAL RESEARCH LAB, and Wolfe, N. L.

Abstract

Nitroreductase and dehalogenase enzymes have been isolated from sediments and soils. Using enzyme linked immunospecific assays (ELISA), a number of aquatic plants have been identified as sources of the enzymes. The plants were then brought back into the laboratory and evaluated as candidates for further remediation studies., Presented at Fourteenth Annual Symposium 1995, Current Topics in Plant Biochemistry, Physiology and Molecular Biology, "Will Plants Have a Role in Bioremediation?", University of Missouri, Columbia, MO, p45-46, April 19-22, 1995.

Published
1995

67. Phytoremediation of Hazardous Wastes

Author

ENVIRONMENTAL PROTECTION AGENCY ATHENS GA ENVIRONMENTAL RESEARCH LAB, McCutcheon, Steven C., Wolfe, N. L., Carreria, Laura H., Ou, Tse-Yuan, ENVIRONMENTAL PROTECTION AGENCY ATHENS GA ENVIRONMENTAL RESEARCH LAB, McCutcheon, Steven C., Wolfe, N. L., Carreria, Laura H., and Ou, Tse-Yuan

Abstract

A new and innovative approach to phytoremediation (the use of plants to degrade hazardous contaminants) was developed. The new approach to phytoremediation involves rigorous pathway analyses, mass balance determinations, and identification of specific enzymes that break down trinitrotoluene (TNT), other explosives (RDX and HMX), nitrobenzene, and chlorinated solvents (e.g., TCE and PCE) (EPA 1994). As a good example, TNT is completely and rapidly degraded by nitroreductase and laccase enzymes. The aromatic ring is broken and the carbon in the ring fragments is incorporated into new plant fiber, as part of the natural lignification process. Half lives for TNT degradation approach 1 hr or less under ideal laboratory conditions. Continuous-flow pilot studies indicate that scale up residence times in created wetlands may be two to three times longer than in laboratory batch studies. The use of created wetlands and land farming techniques guided by rigorous field biochemistry and ecology promises to be a vital part of a newly evolving field, ecological engineering.

Published
1995

73. Biochemical Remediation Using Plant Enzymes

Author

ENVIRONMENTAL PROTECTION AGENCY ATHENS GA ENVIRONMENTAL RESEARCH LAB, Wolfe, N. L., ENVIRONMENTAL PROTECTION AGENCY ATHENS GA ENVIRONMENTAL RESEARCH LAB, and Wolfe, N. L.

Abstract

The transformation of trinitrotoluene (TNT) to environmentally acceptable compounds is achieved through a Lab-developed process that uses common aquatic weeds containing a nitroreductase enzyme. This research breakthrough provides an efficient and inexpensive technology for the cleanup of soils contaminated with munitions waste at military installations and other sites.

Published
1994

74. Installation Restoration Research Program. Alternative Methods for Biological Destruction of TNT: A Preliminary Feasibility Assessment of Enzymatic Degradation.

Author

ARMY ENGINEER WATERWAYS EXPERIMENT STATION VICKSBURG MS, Wolfe, N. L., Ou, Tse-Yuan, Carreira, Laura, Gunnison, Douglas, ARMY ENGINEER WATERWAYS EXPERIMENT STATION VICKSBURG MS, Wolfe, N. L., Ou, Tse-Yuan, Carreira, Laura, and Gunnison, Douglas

Abstract

The ability of a nitrate reductase enzyme extracted from pond sediment to remediate soils contaminated with low levels of TNT was evaluated. The enzyme extract was found to reduce TNT levels in four of the six soils examined over a 35-day incubation period. With more extensive incubation, treatments containing the enzyme extract significantly reduced TNT levels in highly contaminated soils as well. Based on monoclonal antibody investigations of the pond sediment, a stonewort (Nitella sp.) was found to be the source of the nitrate reductase enzyme found in the sediment. Samples of the stonewort were collected and used to treat the same six soils. Stonewort was effective in reducing the TNT concentrations in all six soils. Based on this work, use of the nitrate reductase in highly concentrated form may be effective for treating low to highly contaminated soils in bioreactors. However, to obtain sufficient enzyme for this purpose, the gene responsible for enzyme production will need to be cloned into a rapidly growing microorganism. Use of intact stonewort plant material will be an effective treatment for in situ bioremediation. 2,4,6-Trinitrotoluene, Nitrate reductase, Enzyme, Stonewort, Explosive.

Published
1994

87. Preface

Author

SEIBER, JAMES N., primary, KNUTESON, JAMES A., additional, WOODROW, JAMES E., additional, WOLFE, N. LEE, additional, YATES, MARYLYNN V., additional, and YATES, S. R., additional

Published
1996
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89. BOOK REVIEWS

Author

Croom, Philip, primary, Hoolihan, Joan Thomas, additional, Coggan, Janet M., additional, Wolfe, N. J., additional, Jarvis, Mary, additional, Paquette-Murphy, Mindy R., additional, and Larson, Betsy, additional

Published
1995
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