51. Characterization and Variability of Endotoxin and 3-Hydroxy Fatty Acids in an Office Building During a Particle Intervention Study
- Author
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Donald K. Milton, Cynthia J. Hines, Mark J. Mendell, Lennart Larsson, William J. Fisk, and Martin R. Petersen
- Subjects
Carbon chain ,Environmental Engineering ,Chemistry ,Indoor air ,Endotoxin activity ,Public Health, Environmental and Occupational Health ,Building and Construction ,complex mixtures ,Intervention studies ,Environmental chemistry ,Geometric standard deviation ,Particle ,Spatial variability ,Geometric mean - Abstract
Air and dust samples were collected on two floors of an office building during a double-blind particle intervention study to examine spatial and temporal variability of airborne endotoxin over a period of weeks, and to characterize endotoxin activity and lipopolysaccharide (LPS) content in carpet and chair dust. Air samples were collected on multiple days within and across weeks. Dust samples were collected from carpets and chairs one day per week for three weeks. Endotoxin was measured using a Limulus assay. Dust samples were analyzed for LPS by determination of 3-hydroxy fatty acids (3-OHFAs) using gas chromatography-mass spectrometry. The geometric mean (geometric standard deviation) for 96 indoor air samples was 0.24 (1.6) EU/m3. Significant within-floor spatial variation of airborne endotoxin was found (P
- Published
- 2000