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A sensing measurement scheme based on the optical fiber coupling optical driving converting probe is proposed. Using the optical feedback stabilized 5 mW LD as illuminant, the optical driving distance is 100 m. The power of designed low-consumption temperature-sensitive sensor probe is small to 2.2 mW. The design idea of this new sensor and the key technology of realizing optical driving are introduced in this paper. Experimental results show that the temperature sensor with high resolution can resist high-voltage and electromagnetic interference, and has high sensitivity and precision with 1000 Hz/°C.

A all-fiber perimeter alarm system is designed which is composed of the optical Fiber Sensor Module(FSM) laid along the perimeter and Security Information Control Center(SICC) (including display terminals-the large screen display, the terminal of personnel operation, the system control host, etc.) and optical fiber fence (or sensor board). It uses optical fiber (cable) as the sensing and transmission device, through directly contacting with optical fiber (cable) or through the carrying material, passing various disturbances to the fiber (cable), carrying continuous real-time monitoring and collecting disturbance data. Through the back-end analysis, processing and intelligent identification, it can determine the different types of external disturbances. The system can automatically obtain the real-time alarm information that the optical cable generates and process these warning messages according to predefined business logic, then generate sound and light warning prompt and provide several functions of statistics, query on the warning message.

Non-alcoholic fatty liver disease (NAFLD) is a common cause of chronic hepatitis, which leads to cirrhosis and hepatocellular carcinoma. However, it is difficult to identify subjects at high risk for NAFLD onset. This study aims to construct a model to predict the onset of NAFLD within 2 years in elderly adults.This study included and followed 3378 initial NAFLD-free subjects aged 60 years or over for 2 years, which were randomly divided into a training set and a validation set. NAFLD was diagnosed on ultrasound. Clinical and laboratory data were recorded at baseline. A model was constructed in the training set to predict the onset of NAFLD and validated in the validation set.Body mass index, hemoglobin, fasting blood glucose, and triglycerides were identified as predictors for the onset of NAFLD. A risk score (R) was calculated by them. It classified the subjects into low-risk group (R ≤ -2.88), moderate-risk group (-2.88 R ≤ -1.26), and high-risk group (R -1.26). In the training set, 4.68% of the participants in the low-risk group, 11.59% of the participants in the moderate-risk group, and 31.02% of the participants in the high-risk group developed NAFLD. In the validation set, 5.84% of the participants in the low-risk group, 10.57% of the participants in the moderate-risk group, and 29.44% of the participants in the high-risk group developed NAFLD.This study developed a model to predict the onset of NAFLD in elderly adults, which might provide indications for intervention to these subjects.

The design methods of a kind of three-dimensional fuzzy controller and reasoning rules of its control algorithm, and the relevant simplification technology of three-dimensional table have been advanced in this paper. Self-optimized algorithm of the fuzzy controller has also been presented. The simulation results of high-order non-linear system and test of its practical applications have shown its good control effect.

The function of the herpes simplex virus type 1 (HSV-1) UL4 protein is still elusive. Our objective is to investigate the subcellular transport mechanism of the UL4 protein. In this study, fluorescence microscopy was employed to investigate the subcellular localization of UL4 and characterize the transport mechanism in living cells. By constructing a series of deletion mutants fused with enhanced yellow fluorescent protein (EYFP), the nuclear export signals (NES) of UL4 were for the first time mapped to amino acid residues 178 to 186. In addition, the N-terminal 19 amino acids are identified to be required for the granule-like cytoplasmic pattern of UL4. Furthermore, the UL4 protein was demonstrated to be exported to the cytoplasm through the NES in a chromosomal region maintenance 1 (CRM1)-dependent manner involving RanGTP hydrolysis.

When introduce the wavelet theory to filter the hologram and the reconstructed image in the digital holography, the noise of the reconstructed image is greatly reduced. In the off-axis digital holography, the zero-order wave is decreased dramatically when after a wavelet filter, at the same time, the speckle noise is also reduced, and it turns out that the resolution of the reconstruction image is improved greatly. The system launches the research thoroughly on the three-dimensional body digital holographic technology, from the theory to the application, from the simulation to the experiment, and has elaborated and analyzed each characteristic of three-dimensional body digital holographic technology. It proposes many kinds of improved effective method of three-dimensional body digital holographic restructuring information, and finally has effectively explored the three-dimensional body digital holographic technology application through the experiment.

A set of fluorescence optical fiber temperature measurement system is designed based on Y2O2S:Eu+Fe2O3.The system is mainly composed of the following parts: excitated illuminant, probe, photoelectric detector, belt wave filter, zero checking and fluorescence life-span measurement device. The fluorescence matters give out light when they are stimulated by external light. Fluorescence intensity and life-span depend on the fluorophore temperature. Fluorescence life-span is fluorescence materials’ inherence characteristic, we can realize temperature's absolut measurement by carrying out this measurement, and it has very good sensitiveness and measure accuracy within the low and middle temperature range. Fluorescence frequency is identical with exciting light since exciting light is modulated to a fixed frequency. Y2O2S:Eu+Fe2O3is a new material handled by Y2O2S:Eu with red pigment of ferric oxide, while the corresponding wave crest’s intensity of Y2O2S:Eu+Fe2O3is 9 times higher than Y2O2S:Eu nearly a magnitude. The experiment of the optical spectrum to several sensitive materials confirms the match that Y2O2S:Eu+Fe2O3is with light source and the feasibility of the system.

To study the chemical constituents from Carpesium abrotanoides.Compounds were isolated and pu- rified by silica gel and Sephadex LH-20 column chromatography, their structures were determined by using spectroscopic analysis.Ten compounds were isolated and identified as Vomifoliol (I), 2-Desoxy-4-epi-pulchellin (II), 8-epi-Confertin (III), 1-epi-Inuviscolide (IV), Telekin (V), Isotelekin (VI), 4 (15)-β-Epoxyisotelekin (VII), Carabrone (VIII), Carabrol (IX), and 3- Deuteriomethyl-5-methyl-2,3-dihydrobenzofuran (X).Compounds I, III, IV,VII, VII and X are isolated from this plant for the first time.

To explore the clinical manifestations and short-term prognosis of twin-twin transfusion syndrome (TTTS) in neonates with different disease stages, receiving different intrauterine interventions, or as blood donors and recipients.The study retrospectively collected 76 TTTS neonates who were hospitalized in the Neonatal Ward, Peking University Third Hospital. The participants were classified into mild TTTS (n=38) and severe TTTS groups (n=21), or into amnioreduction (n=20), laser surgery (n=21), and expectant therapy groups (n=32), or into donor (n=23) and recipient groups (n=30).The severe TTTS group had higher incidences of brain injury, heart disease, asphyxia, and renal damage and in-hospital mortality rate compared with the mild TTTS group, but the differences had no statistical significance. The laser surgery group displayed decreasing trends in the incidences of brain injury, heart disease, and renal damage and in-hospital mortality rate compared with the amnioreduction and expectant therapy groups. The recipient group had higher incidences of heart diseases and pathological jaundice than the donor group (P0.05). The donor group had higher incidences of asphyxia and renal damage than the recipient group, but with no significant difference.The neonates with severe TTTS have higher rates of organ damages and in-hospital mortality. Intrauterine laser surgery seems to lead to a better prognosis compared with the amnioreduction and expectant therapy. The recipients are more susceptible to heart diseases and pathological jaundice, whereas the donors are more susceptible to asphyxia and renal damage.

To study the chemical constituents of Zanthoxyli Cortex.The chemical constituents were isolated and purified by silica gel and HP-20, MCI gel, Sephadex LH -20 column chromatography, RP-18 and PTLC. Their structures were elucidated by the analysis of spectral data and chemical properties.Ten compounds were isolated from EtOAc extract and their structures were identified as: asarinin (I), fargesin (II), eudesmin (III), (1R, 2R, 5R, 6S)-2-(3,4-dimethoxyphenyl)-6-(3,4-dihydroxyphenyl)-3,7-dioxabicyclo[3.3.0]-octane(IV), dimethoxysamin(V), rel-(1R,5R,6S)-6-(3,4-dimethoxyphen-yl)-3,7-dioxabicyclo-[3.3.0]-octan-2-one(VI), Magnone A(VII), beta-sitoste-rol( VIII), beta-armyrin(IX), beta-amyrone(X).These compounds isolated from Zanthoxyli Cortex's Ethyl acetate extract are all known compounds. Fargesin(II) and beta-amyrone(X) are isolated from Zanthoxyli Cortex for the first time.

Investigation of the bark of Zanthoxylum simulans afforded six new dimeric lignans zanthpodocarpins C-H (1-6) bearing an unusual alpha,beta-unsaturated ketone group. The new structures of 1-6 were determined by using detailed spectroscopic analysis. All of the isolated compounds were examined for their inhibitory effects against rat joint synovial cell and splenocyte proliferation. Compounds 1-6 showed potent anti-inflammatory activities with IC50 values ranging from 18.6 to 36.1 mu M, and 13.8 to 74.3 mu M. (C) 2014 Elsevier Ltd. All rights reserved.

A sapphire fiber thermal probe with Cr3+ ion-doped end was grown using the laser heated pedestal method. The fluorescence thermal probe offers advantages of compact structure, high performance and the ability to sustain high temperature from the room temperature to 450 °C. Based on the fast fourier transform (FFT), the fluorescence lifetime is obtained from the tangent function of the phase angle of the first non-zeroth item of FFT result. Compared with other traditional fitting methods, our method has advantages such as fast speed, high accuracy and being free from the influence of the base signal. The standard deviation of FFT method is about half of that of the Prony method and close to the one of the Marquardt method. In addition, since the FFT method is immunity to the background noise of the signal, the background noise analysis can be skipped.

The role of DAXX in ovarian cancer development and metastasis has not been investigated before now.Overexpression of DAXX enhanced ovarian cancer cell proliferation, colony formation, and migration, whereas Daxx depletion had the opposite effects.DAXX promotes ovarian cancer cell proliferation and chemoresistance.ModulatingDAXXmay be an effective strategy for preventing the recurrence and chemoresistance of ovarian cancers. Understanding the genes involved in apoptosis and DNA damage responses may improve therapeutic strategies for ovarian cancer. The death domain-associated protein DAXX can be either a pro-apoptotic or an anti-apoptotic factor, depending on the cell type and context. In this study, we found that DAXX was highly expressed in human ovarian surface epithelial tumors but not in granulosa cell tumors. In cultured ovarian cancer cells, DAXX interacted with promyelocytic leukemia protein (PML) and localized to subnuclear domains (so-called PML nuclear bodies). A role for DAXX in ovarian cancer cell proliferation, metastasis, and radio/chemoresistance was examined. Overexpression of DAXX enhanced multiple ovarian cancer cell lines' proliferation, colony formation, and migration, whereas Daxx depletion by RNA interference had the opposite effects. When transplanted into nude mice, ovarian cancer cells that overexpressed DAXX displayed enhanced tumorigenesis capability in vivo, whereas Daxx depletion inhibited tumor development. Importantly, Daxx induced tumorigenic transformation of normal ovarian surface epithelial cells. Daxx also protected ovarian cancer cells against x-irradiation- and chemotherapy-induced DNA damage by interacting with PML. Taken together, our results suggest that DAXX is a novel ovarian cancer oncogene that promotes ovarian cancer cell proliferation and chemoresistance in ovarian cancer cells. Thus, modulating DAXX-PML nuclear body activity may be an effective strategy for preventing the recurrence and chemoresistance of ovarian cancers.

In Drosophila melanogasler,the wings apart-like (wapl) gene encodes a protein that regulates heterochromatin structure. Human wapl gene is human homolognue of wapl,they have same function. HWAPL is a cohesion-binding protein that affect chromosomal segregation. It has a character of oncoproteins and linked to cervical carcinogenesis and tumor progression.

ERP project post evaluation EAIU (Evaluate Analyze Improve Upgrade) cycling model were demonstrated in this thesis. ERP project success degree evaluation system was designed. The result of ERP project success degree evaluation could be obtained through Delphi weight establishment method and comprehensive multi-level fuzzy evaluation. Second, data Envelopment Analysis was used to analyze ERP post evaluation. Enterprise Resource Planning project post evaluation Data Envelopment Analysis Model was developed to judge whether the ERP project was DEA effective or not. At last, through the contrastive analysis of radar charts, the result of evaluation looks compact.

As a new mode of governance, e-government is a challenge to the traditional government management. The E-government is a deep change to the traditional management model by using modern network technology and information technology. It not only means the further disclosure and transparent of governmental management, but also means the change of traditional function and the reconstruction of the organizational structure. From global point of view, the development of e-government and government management model changes have always been associated with each other. It has the outstanding performance in the government power system reconstruction, governmental organization restructuring; the change of government management and so on. With the main issues in the building of Chinese e-government systems, the model and framework of an e-government system has been designed, which is both macro-guidance and specific system implementation for the development of Chinese e-government strategy. We explore the implementation of e-government management on China in promoting.

Economy globalization and the development of information technology have intensified competition among modern companies, while customer relationship has became an important resource for building up core competence. Customer Relationship Management(CRM), as a new idea, technology and strategy, provides an all-around guidance and support for business to build, keep and manage customer relationship. CRM and its application are proposed according to the principle“analyzing CRM layer upon layer, applying CRM step by step, understanding CRM completely, implementing CRM holistically.” Holding the principle that idea is base of CRM, its technology is tools and its strategy is the key. Aiming at certain problems in the application of CRM, studying CRM idea, introducing technology and enact strategy have been brought forward.

The recombined adenovirus DNA was transfected into 293 cells for packing and amplification of replication-deficient Ad-CMV-E6/E7, Ad-K14 -E6/E7 virus was purified by CsCl density gradient centrifugation , recombined adenovirus Ad-CMV-E6/E7, Ad-K14 -E6/E7 were used as experimental group, while pAd-CMV and pAdtrack-K14 were used as control group. Four of them were injected through one main vein of nude mice tail respectively. These mice were then treated with 0.05 mg 17beta-estradiol over 12 weeks. Mice were anaesthesiaed with 2.5% Avertint and the vagina, mammary gland, ovaries and uterus were dissected and fixed in 3.75% paraformaldehyde overnight at 4 degrees C. Paraffin-embedded sections, HE staining and identification of P53 and Bcl-2 protein via immunohistochemistry were performed. The expression of E6/E7 was verified by RT-PCR in different tissue of nude mice. HE staining showed evident hyperplasy in cervix-uterus transformation zone of experimental group 2. The expression of mutant P53 and Bcl-2 were higher than control group via immunohistochemical S-P method in uterus stroma-cell. Western blotting also showed that E6 protein was expressed. The expression of E6/E7 was higher than control group by human cytokeratin promoter 14 and hyperlasy changes were detected in epithelial tissue of cervix-uterus transformation zone.

A sapphire fiber thermometer for high temperature measurement is described. The mechanism of the fluorescence temperature measurement is based on the basic physical phenomenon of photoluminescence. The so-called photoluminescence essentially is the superthermal radiation luminescence when some materials are stimulated by ultraviolet, visible or a certain form of the infrared electromagnetic radiation. The fluorescence lifespan and fluorescence strength of all fluorescence materials will imply some temperature correlation within some homologous temperatures. It uses a specially grown sapphire fiber as thermometer probe. The end part of the sapphire fiber is doped with Cr3+ion by means of the laser heat pedestal growth (LHPG) method and coated with some high radiant material to constitute a mini fiber cavity. The fluorescence thermal probe offers the advantages of a compact structure, a high performance and the ability to withstand high temperatures in detection. According to the surface temperature, we can obtain the inner temperature by the temperature field. It can be used to measure the steel fluid of the tundish in continuous cast steel machines. An experimental system of the thermometer and the preliminary experimental results are presented.