62 results on '"Wang, Meng‐Yue"'
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52. [Construction strategies and prospect of the Global Medicinal Plant Stem Cell Bank].
- Author
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Xiang L, Gao RR, Wang MY, Xiao LY, Liu YZ, and Chen SL
- Subjects
- Conservation of Natural Resources, Ethnicity, Humans, Medicine, Chinese Traditional, Stem Cells, Plants, Medicinal
- Abstract
Medicinal plant stem cells are separated from the meristem and vascular cambium of medicinal plants, which can produce active components for preventing and treating diseases and improving body physical functions under certain conditions. Medicinal plant stem cells come from a broad category of medicinal plants, including ethnic medicinal plants, folk medicinal plants, original plants of health products, vegetables, fruits, and other potential medicinal plants. At present, the techniques for the isolation, identification, preservation and culture of medicinal plant stem cells have become increasingly mature, and the mechanism of stem cell differentiation, growth and regulation of secondary metabolites has been studied in depth. Medicinal plant stem cells have a broad application prospect in medicine, health food, food and medical beauty products. As a strategic resource, the construction of the "Global Medicinal Plant Stem Cell Bank" was first proposed to preserve various kinds of medicinal plant resources in the world, and it will go global relying on the internationalization strategy of traditional Chinese medicine. The bank should follow safety, environmental protection, advanced and practical design principles. The main construction contents include the original plant bank, stem cell bank, component resource bank, gene bank, database and resource sharing system, with genetic and data resources incorporated into the scope of protection and utilization. The bank will establish a new strategy for medicinal plant resources protection and regeneration, and provide a new resource for natural products drug discovery and a technology sharing platform for various medicinal plant stem cells. As a resource treasury, a source of innovative technologies and a center of cooperation, it will become the core driving force of the global medicinal plant stem cell industry.
- Published
- 2022
- Full Text
- View/download PDF
53. Switchable, Reagent-Controlled C(sp 3 )-H Selective Iodination and Acetoxylation of 8-Methylquinolines.
- Author
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Zhang ML, Zhang XL, Guo RL, Wang MY, Zhao BY, Yang JH, Jia Q, and Wang YQ
- Abstract
An efficient Pd-catalyzed C(sp
3 )-H selective iodination of 8-methylquinolines is reported herein for the first time. Because of the versatility of organic iodides, the method offers a facile access to various C8-substituted quinolines. By slightly switching the reaction conditions, an efficient C(sp3 )-H acetoxylation of 8-methylquinolines has also been enabled. Both approaches feature mild reaction conditions, good tolerance of functional groups, and a broad substrate scope.- Published
- 2022
- Full Text
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54. Increased activation of the caudate nucleus and parahippocampal gyrus in Parkinson's disease patients with dysphagia after repetitive transcranial magnetic stimulation: a case-control study.
- Author
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Huang PL, Wang SJ, Sun RF, Zhu ZM, Li XL, Li WS, Wang MY, Lin M, and Gong WJ
- Abstract
Repetitive transcranial magnetic stimulation (rTMS) has been shown to effectively improve impaired swallowing in Parkinson's disease (PD) patients with dysphagia. However, little is known about how rTMS affects the corresponding brain regions in this patient group. In this case-control study, we examined data from 38 PD patients with dysphagia who received treatment at Beijing Rehabilitation Medicine Academy, Capital Medical University. The patients received high-frequency rTMS of the motor cortex once per day for 10 successive days. Changes in brain activation were compared via functional magnetic resonance imaging in PD patients with dysphagia and healthy controls. The results revealed that before treatment, PD patients with dysphagia showed greater activation in the precentral gyrus, supplementary motor area, and cerebellum compared with healthy controls, and this enhanced activation was weakened after treatment. Furthermore, before treatment, PD patients with dysphagia exhibited decreased activation in the parahippocampal gyrus, caudate nucleus, and left thalamus compared with healthy controls, and this activation increased after treatment. In addition, PD patients with dysphagia reported improved subjective swallowing sensations after rTMS. These findings suggest that swallowing function in PD patients with dysphagia improved after rTMS of the motor cortex. This may have been due to enhanced activation of the caudate nucleus and parahippocampal gyrus. The study protocol was approved by the Ethics Committee of Beijing Rehabilitation Hospital of Capital Medical University (approval No. 2018bkky017) on March 6, 2018 and was registered with Chinese Clinical Trial Registry (registration No. ChiCTR 1800017207) on July 18, 2018., Competing Interests: None
- Published
- 2022
- Full Text
- View/download PDF
55. [Establishment and verification of quality evaluation method of Jizhi Syrup based on quantitative analysis of multi-components by single marker].
- Author
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Ding HR, Liu F, Wang MY, and Li XB
- Subjects
- Chromatography, High Pressure Liquid methods, Ephedrine, Reproducibility of Results, Drugs, Chinese Herbal chemistry, Glycyrrhizic Acid analysis
- Abstract
A quantitative analysis of multi-components by single marker(QAMS) method was established for the simultaneous determination of ephedrine hydrochloride, protocatechuic acid, 5-caffeoylquinic acid, 4-hydroxybenzoic acid, naringin, neohesperidin, glycyrrhizic acid, and praeruptorin A in Jizhi Syrup by high performance liquid chromatography(HPLC) with ultraviolet multi-wavelength detection system, and its feasibility in quality evaluation of Jizhi syrup was verified. With naringin as the internal reference substance, the relative correction factors and chromatographic peak localization methods of other seven components were respectively established at 210, 254, 280, and 320 nm. The method reproducibility was validated, and the result of QAMS were compared with those obtained by the external standard method(ESM) to verify the accuracy and feasibility of the method. The relative correction factors of ephedrine hydrochloride, protocatechuic acid, 5-caffeoylquinic acid, 4-hydroxybenzoic acid, neohesperidin, glycyrrhizic acid, and praeruptorin A with naringin as reference were 0.846, 0.582, 0.608, 0.293, 0.913, 2.207, and 0.940, respectively, which presented excellent reproducibility under different experimental conditions. Furthermore, QAMS and ESM showed no significant difference in the results for 15 batches of samples. Except protocatechuic acid and 5-caffeoylquinic acid, other six compounds were the exclusive components of single medicinal materials. In addition, glycyrrhizic acid and praeruptorin A were identified in the Jizhi Syrup for the first time, filling up the blank of no component detected in Glycyrrhizae Radix et Rhizoma and Peucedani Radix. The method established in this study is convenient, efficient, specific, accurate, and reliable, which can comprehensively and effectively evaluate the quality of Jizhi Syrup to ensure the safety and efficacy of this drug in clinical application.
- Published
- 2022
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56. [Screening of reference genes for quantitative real-time PCR in Artemisia argyi].
- Author
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Yi XZ, Wu L, Xiang L, Wang MY, Chen SL, Shi YH, and Liu X
- Subjects
- Gene Expression Profiling, Genes, Plant genetics, Plant Leaves genetics, Real-Time Polymerase Chain Reaction, Reference Standards, Transcriptome, Artemisia genetics
- Abstract
Artemisia Argyi Folium, a traditional Chinese medicine of important medicinal and economic value, sees increasing demand in medicinal and moxibustion product market. Screening stable and reliable reference genes for quantitative real-time PCR(qRT-PCR) is a prerequisite for the analysis of gene expression in Artemisia argyi. In this study, eight commonly used reference genes, Actin, 18s, EF-1α, GAPDH, SAND, PAL, TUA, and TUB, from the transcriptome of A. argyi, were selected as candidate genes. The expression of each gene in different tissues(roots, stems, and leaves) of A. argyi and in leaves of A. argyi after treatment with methyl jasmonate(MeJA) for different time(0, 4, 8, 12 h) was detected by qRT-PCR. Then, geNorm, NormFinder, BestKeeper, ΔCT, and RefFinder were employed to evaluate their expression stability. The results demonstrated that Actin was the most stable reference gene in different tissues and in leaves treated with MeJA, and coming in the second was SAND. Furthermore, the expression of DXS and MCT which are involved in terpenoid backbone biosynthesis was detected in different tissues and after MeJA treatment. The results showed that the expression patterns of DXS and MCT in different tissues and under MeJA treatment calculated with Actin and SAND as internal reference genes were consistent, which validated the screening results. In conclusion, Actin is the most suitable reference gene for the analysis of gene expression in different tissues of A. argyi and after MeJA treatment. This study provides valuable information for gene expression analysis in A. argyi and lays a foundation for further research on molecular mechanism of quality formation of Artemisia Argyi Folium.
- Published
- 2022
- Full Text
- View/download PDF
57. [Active constituents of Urtica fissa in inhibition of benign prostatic hyperplasia].
- Author
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Zhang H, Chen Y, Li XB, Deng WX, and Wang MY
- Subjects
- Animals, Plant Extracts chemistry, Plant Extracts pharmacology, Rats, Prostatic Hyperplasia drug therapy, Urticaceae chemistry
- Abstract
The present study investigated the material basis of Urtica fissa for the inhibition of benign prostatic hyperplasia(BPH). The active fractions were screened, and the extracts of dichloromethane and ethyl acetate exhibited significantly inhibitory activities against 5α-reductase in vitro and BPH in model rats. The chemical constituents in the active fractions were systematically investigated, and 28 compounds were obtained, which were identified as lobechine methyl ester(1), dibutyl-O-phthalate(2), 1-monolinolein(3), epipinoresinol(4), 5-hydroxy-3,4-dimethyl-5-pentanyl-2(5H)-furanone(5), E-7,9-diene-11-methenyl palmitic acid(6), evofolin B(7), ficusal(8), threo-2,3-bis-(4-hydroxy-3-methoxyphenyl)-3-ethoxypropan-1-ol(9), α-viniferin(10),(9R,7E)-9-hydroxy-5,7-mengatigmadien-4-one-9-O-β-D-glucopyranoside(11), indole-3-carboxaldehyde(12), p-hydroxy ethyl cinnamate(13), benzyl alcohol-O-β-D-glucoside(14), L-methionine(15), 4-methoxyaniline(16), 6-aminopurine(17), 8'-acetyl oilvil(18), 4-methoxyl-8'-acetyl oilvil(19), vanillic acid(20), β-hydroxypropiovanillone(21), 7-hydroxy-6-methoxycoumarin(22), p-hydroxybenzaldehyde(23), pinoresinol(24), erythro-1,2-bis-(4-hydroxy-3-methoxyphenyl)-1,3-propanediol(25), urticol(26), urticol-7-O-β-D-glucopyranoside(27), and lobechine(28). Compounds 1-17 were isolated from U. fissa for the first time. Meanwhile, compound 1 was a new natural product. Compounds 10, 11, 19, 21, and 27 exhibited significant inhibitory effects on 5α-reductase.
- Published
- 2022
- Full Text
- View/download PDF
58. [Lignans with inhibitory effect on 5α-reductase from Urtica cannabina].
- Author
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Chen Y, Guo ZX, Li XB, Sun CJ, and Wang MY
- Subjects
- 5-alpha Reductase Inhibitors, Chromatography, High Pressure Liquid, Magnetic Resonance Spectroscopy, Molecular Structure, Cholestenone 5 alpha-Reductase pharmacology, Lignans pharmacology, Urticaceae drug effects, Urticaceae enzymology
- Abstract
The lignans in Urtica cannabina were isolated by preparative HPLC, silica, and ODS column chromatographies, and identified by NMR and HR-MS. The inhibitory activities on 5α-reductase were evaluated in vitro. As a result, ten secolignans,(2R,4S)-2,4-bis(3-methoxyl-4-hydroxyphenyl)-3-butoxypropanol(1), 3,4-trans-3-hydroxymethyl-4-[bis(3,4-dimethoxyphenyl)methyl] butyrolactone(2), 3,4-trans-3-hydroxymethyl-4-[(3,4-dimethoxyphenyl)(3-methoxyl-4-hydroxyphenyl)methyl] butyrolactone(3), 3,4-trans-3-hydroxymethyl-4-[bis(3-methoxyl-4-hydroxyphenyl)methyl] butyrolactone(trans urticol, 4), 3,4-trans-3-hydroxymethyl-4-[bis(3,4-dimethoxyphenyl)methyl] butyrolactone-3-O-β-D-glucopyranoside(5), 3,4-trans-3-hydroxymethyl-4-[(3,4-dimethoxyphenyl)(3-methoxyl-4-hydroxyphenyl)methyl]butyrolactone-3-O-β-D-glucopyranoside(6), 3,4-trans-3-hydroxymethyl-4-[bis(3-methoxyl-4-hydroxyphenyl)methyl]butyrolactone-3-O-β-D-glucopyranoside(trans-urticol-7-O-β-D-glucopyranoside, 7), cycloolivil-4-O-β-D-glucopyranoside(8), isolariciresinol-4'-O-β-D-glucopyranoside(9), and olivil-4'-O-β-D-glucopyranoside(10), together with a polyphenol [α-viniferin(11)], were isolated from U. cannabina for the first time. Compound 1 was a new lignan. Compound 7 was potent in inhibiting 5α-reductase.
- Published
- 2021
- Full Text
- View/download PDF
59. New lignans from Urticae Fissae Herba.
- Author
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Wang MY, Zhang Y, Zhang H, Feng XR, and Li XB
- Subjects
- Animals, China, Medicine, Chinese Traditional, Mice, Molecular Structure, Nitric Oxide antagonists & inhibitors, Plant Components, Aerial chemistry, Plant Extracts chemistry, Plant Extracts pharmacology, RAW 264.7 Cells, Tumor Necrosis Factor-alpha metabolism, Anti-Inflammatory Agents, Non-Steroidal chemistry, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Lignans chemistry, Lignans pharmacology, Urticaceae chemistry
- Abstract
Two new lignans named neourticol A (1) and neourticol B (2), together with seven known compounds (3-9), were isolated from Urticae Fissae Herba, a folk medicine for rheumatism arthritis in China. The active evaluation results showed that 1 and 2 possessed the potent anti-complement and anti-inflammatory activities.
- Published
- 2019
- Full Text
- View/download PDF
60. [Studies on liposoluble constituents from Momordicae Semen].
- Author
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Wang MY, Zhan ZB, Xiong Y, and Li XB
- Subjects
- Chromatography, High Pressure Liquid, Magnetic Resonance Spectroscopy, Phytochemicals isolation & purification, Drugs, Chinese Herbal chemistry, Momordica chemistry, Phytochemicals chemistry
- Abstract
The liposoluble constituents in Momordicae Semen were investigated in the present study. By silica gel, Sephadex LH-20 column chromatography, and semi-preparative HPLC, 22 compounds were isolated and purified from dichloromethane and ethyl acetate fraction. Based on NMR and MS spectra analyses, these compounds were identified as lupeol (1), 5-(1'-hydroxypentyl)-5H-furan-2-one (2), palmitic acid (3), viscumamide (4), clavatustide C (5), laxanol (6), threo-1-(4-hydroxyphenyl)-2-{4-[2-formyl-(E)-vinyl]-2-methoxyphenoxyl}-propane-1, 3-diol (7), α-spinasterol-3-O-β-D-glucoside (8), chushizisin F (9), ehletianol C (10), tanegool (11), (7R, 8R, 8'R)-4'-guaiacylglyceryl-evofolin B (12), ligballinone (13), (7R, 8S, 8'R)- 4, 4', 9-trihydroxy- 7, 9'-epoxy- 8, 8'-lignan (14), chushizisin I (15), chushizisin A (16), chushizisin G (17), p-coumaraldehyde (18), α-spinasterol (19), p-hydroxybenzoic acid (20), chushizisin E (21), and 3-[2-(4-hydroxyphenyl)-3-hydroxyphenyl-2, 3-dihydro-1-benzofuran-5-yl] propane-1-ol (22), respectively. Compounds 1-17 were isolated from Momordica cochinchinensis for the first time. Compound 2 was a new natural product while compounds 4 and 5 were first found in the terrestrial organism., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2018
- Full Text
- View/download PDF
61. [Quality standard study on Entadae Semen].
- Author
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Shen HP, Wang MY, and Li XB
- Subjects
- China, Chromatography, High Pressure Liquid, Chromatography, Thin Layer, Drugs, Chinese Herbal isolation & purification, Drugs, Chinese Herbal standards, Quality Control, Drugs, Chinese Herbal chemistry, Fabaceae chemistry
- Abstract
Objective: To establish the quality standard of Entadae Semen, and provide scientific basis for its quality control., Method: TLC and HPLC were used for qualitative identification and quantitative analysis of phaseoloidin and entadamide A-O-β-D-glucopyranoside in Entadae Semen. The test of water content, ash and ethanol-soluble extractives of Entadae Semen was carried out according to the methods recorded in appendix of Chinese Pharmacopeia (2010 edition)., Result: The TLC was well separated with clear spots. The linear range of phaseoloidin was between 0.014 to 2.747 g x L(-1) (r = 0.999 6, n = 9) with an average recovery rate of 101.06% (RSD 0.90%, n = 6); the linear range of entadamide A-O-β-D-glucopyranoside was between 0.002 to 0.452 g x L(-1) (r = 0.999 7, n = 9) withan average recovery rate of 101.52% (RSD 1.09%, n = 6). The content of phaseoloidin in sample is between 5.12% to 9.24%, entadamide A-O-β-D-glucopyranoside is between 0.55% to 2.17%, alcohol-soluble extracts is between 30.9% to 45.2%, water is between 6.6% to 8.6%, and total ash is between 2.4% to 2.9%., Conclusion: The established standard is acceptable for quality control of Entadae Semen.
- Published
- 2015
62. [Investigation of enhanced low carbon wastewater denitrification by catalytic iron].
- Author
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Wang MY and Ma LM
- Subjects
- Bioreactors, Carbon chemistry, Nitrates chemistry, Nitrites chemistry, Nitrogen chemistry, Nitrous Oxide chemistry, Oxygen chemistry, Denitrification, Iron chemistry, Wastewater, Water Purification methods
- Abstract
The nitrogen removal efficiency and N2O production during the process of coupling catalytic iron and biological denitrification for low C/N ratio wastewater were studied. The results showed that biological denitrification coupled with catalytic iron could significantly improve nitrate transforming efficiency, but led to nitrite accumulation, resulting in little difference in total nitrogen removal efficiency, compared with traditional biological denitrification. N2O production from the catalytic iron group was obviously higher than the traditional control group, among which chemical reaction between ferrous oxide and nitrite had great contribution. However, the highest amount of accumulated N2O was less than 8% of the removed nitrate, and N2O was further reduced to N2 by microorganisms. Furthermore, catalytic iron group could eliminate dissolved oxygen and lower the oxidation reduction potential, which is beneficial for keeping anoxic conditions during denitrification.
- Published
- 2014
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