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438 results on '"Vrtala, Susanne"'

51. Expression in Escherichia coli and Purification of Folded rDer p 20, the Arginine Kinase From Dermatophagoides pteronyssinus: A Possible Biomarker for Allergic Asthma

Author

Sarzsinszky, Eszter, primary, Lupinek, Christian, additional, Vrtala, Susanne, additional, Huang, Huey-Jy, additional, Hofer, Gerhard, additional, Keller, Walter, additional, Chen, Kuan-Wei, additional, Panaitescu, Carmen Bunu, additional, Resch-Marat, Yvonne, additional, Zieglmayer, Petra, additional, Zieglmayer, René, additional, Lemell, Patrick, additional, Horak, Friedrich, additional, Duchêne, Michael, additional, and Valenta, Rudolf, additional

Published
2021
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52. Molecular sensitization profile of asthmatic children in equatorial Africa

Author

Josefina Zakzuk, Vrtala Susanne, Carole Wurmser-Danion, Caraballo Luis, Antoine Roos, Gabrielle Pauli, Cosme Kokou, Huey-Jy Huang, Valenta Rudolf, and Christian Lupinek

Subjects
Pulmonary and Respiratory Medicine, Asthmatic children, lcsh:Immunologic diseases. Allergy, medicine.anatomical_structure, business.industry, Immunology, medicine, Immunology and Allergy, business, lcsh:RC581-607, Sensitization
Published
2020

53. Additional file 1 of Variation in IgE binding potencies of seven Artemisia species depending on content of major allergens

Author

Zhao, Lan, Wanyi Fu, Biyuan Gao, Liu, Yi, Shandong Wu, Chen, Zhi, Xianqi Zhang, Huiying Wang, Feng, Yan, Xueyan Wang, Hongtian Wang, Tianfei Lan, Meiling Liu, Xuefeng Wang, Yuemei Sun, Fangmei Luo, Gadermaier, Gabriele, Ferreira, Fatima, Versteeg, Serge A., Akkerdaas, Jaap H., Deyun Wang, Valenta, Rudolf, Vrtala, Susanne, Gao, Zhongshan, and Ree, Ronald Van

Abstract

Additional file 1: Table S1. Clinical and demographic data of 150 mugwort pollen-allergic individuals sIgE against mugwort extract (w6), Art v 1 (w231) and Art v 3(w233) determined by ImmunoCAP, ND, not determined. AS asthma; AR allergic rhinitis; C, conjunctivitis; E, eczema. I-1, I-2, I-3, I-4 indicates the patients serum used in ImmunoCAP inhibition assay belonging to four groups of different sensitization patterns (1, Art v 1 and Art v 3 positive; 2, Art v 1 positive, Art v 3 negative; 3, Art v 1 negative, Art v 3 positive; 4, Art v 1 and Art v 3 negative). The 82 patients reported in previously studies17, 20, 28 are indicated by an asterisk. Table S2. GenBank accession numbers for three allergen groups in seven Artemisia species.Table S3. Productivity of the three group allergens purified by specific mAb. Table S4. ImmunoCAP IgE characterization of serum pools from five areas. Figure S1. Six Artemisia species collected from China. Figure S2. SDS-PAGE of natural purified Art v 1, Art v 2 and Art v 3 homologous allergens from six Chinese Artemisia species. a, natural Art v 1 homologues purified by specific mAb A7-G4-E6; b, natural Art v 2 homologues purified by specific mAb C9-C1 shown in six different gels; c, natural Art v 3 homologues purified by specific mAb A2-B8. Figure S3. Mass spectra of natural purified Art v 1(a), Art v 2(b) and Art v 3(c) homologues. The peptides verified by LC–MS/MS are shown in red and highlighted. Figure S4. ELISA quantification of three allergen components in Artemisia spp. pollen. a, Chinese silver mugwort (A. argyi) pollen extract (ArE) in SDS gel and reaction to polyclonal antibodies (pAb) by Western blot; b, ELISA standard curve for Art v 1 allergen (mAb A7-G4-E6 and rabbit pAbs); c, ELISA standard curve for Art v 2 homologous allergen (mAb C9-C1 with rabbit pAbs); d, ELISA standard curve for Art v 3 homologous allergen with two mAbs (mAbs A2-B8 and A9-G10) with representative different isoforms. Figure S5..Inhibition of IgE binding to Art v 1 and Art v 3 with seven pollen extracts using two serum pools. a: inhibition ELISA coated with rArt v 1.0101, serum pool from Datong, Shanxi (nArt v 1: 11 kUA/l); b: inhibition ELISA coated with rArt v 1.0101, serum pool from Yantai, Shandong (nArt v 1: 6.51 kUA/l); c: inhibition ELISA coated with rArt v 3.0201, serum pool from Datong, Shanxi (CAP nArt v 3: 11.27 kUA/l); d: inhibition ELISA coated with rArt v 3.0201, serum pool from Yantai, Shandong (CAP nArt v 3: 9.65 kUA/l). Figure S6. Inhibition of sera pool from Shanxi with extract from different species at 100 μg/ml in ELISA coated with 10 μg/ml of different pollen extracts and mixture. 6-mix, mixture of six Artemisia spp. extract in the same proportions.

Published
2020
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66. The Molecular Allergen Recognition Profile in China as Basis for Allergen-Specific Immunotherapy.

Author

D'souza, Nishelle, Weber, Milena, Sarzsinszky, Eszter, Vrtala, Susanne, Curin, Mirela, Schaar, Mirjam, Garib, Victoria, Focke-Tejkl, Margarete, Li, Yanqiu, Jones, Richard, Chen, Hao, Valenta, Rudolf, and Sun, Baoqing

Subjects
MOLECULAR recognition, IMMUNOGLOBULIN E, ANAPHYLAXIS, ALLERGIES, RESPIRATORY allergy, PEANUT allergy
Abstract

Approximately 30% of the world population suffers from immunoglobulin-E (IgE)-mediated allergy. IgE-mediated allergy affects the respiratory tract, the skin and the gastrointestinal tract and may lead to life-threatening acute systemic manifestations such as anaphylactic shock. The symptoms of allergy are mediated by IgE-recognition of causative allergen molecules from different allergen sources. Today, molecular allergy diagnosis allows determining the disease-causing allergens to develop allergen-specific concepts for prevention and treatment of allergy. Allergen-specific preventive and therapeutic strategies include allergen avoidance, vaccination, and tolerance induction. The implementation of these preventive and therapeutic strategies requires a detailed knowledge of the relevant allergen molecules affecting a given population. China is the world´s most populous country with around 1.4 billion inhabitants and an estimated number of more than 400 million allergic patients. Research in allergy in China has dramatically increased in the last decade. We summarize in this review article what is known about the dominating allergen sources and allergen molecules in China and what further investigations could be performed to draw a molecular map of IgE sensitization for China as a basis for the implementation of systematic and rational allergen-specific preventive and therapeutic strategies to combat allergic diseases in this country. [ABSTRACT FROM AUTHOR]

Published
2021
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74. Recombinant Allergens

Author

Valenta, Rudolf, primary, Laffer, Sylvia, additional, Vrtala, Susanne, additional, Grönlund, Hans, additional, Elfman, Lena, additional, Sperr, Wolfgang R., additional, Valent, Peter, additional, Ferreira, Fatima, additional, Mayer, Peter, additional, Liehl, Ekke, additional, Heiss, Susanne, additional, Steiner, Renate, additional, Eichler, Hans Georg, additional, Susani, Markus, additional, and Kraft, Dietrich, additional

Published
1996
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78. Allergen-specific IgE levels and the ability of IgE-allergen complexes to cross-link determine the extent of CD23-mediated T-cell activation

Author

Villazala-Merino, Sergio, Rodriguez-Dominguez, Azahara, Stanek, Victoria, Campion, Nicholas J., Gattinger, Pia, Hofer, Gerhard, Froeschl, Renate, Fae, Ingrid, Lupinek, Christian, Vrtala, Susanne, Breiteneder, Heimo, Keller, Walter, Perkmann, Thomas, Nakamura, Ryosuke, Pickl, Winfried F., Valenta, Rudolf, Eckl-Dorna, Julia, and Niederberger, Verena

Subjects
Adult, Male, Antigen Presentation, IgE-allergen complex, Receptors, IgE, Allergen, T-Lymphocytes, Rhinitis, Allergic, Seasonal, chemical and pharmacologic phenomena, hemic and immune systems, Antigens, Plant, Immunoglobulin E, Middle Aged, Lymphocyte Activation, Article, facilitated allergen presentation, immune system diseases, Bet v 1, T-cell activation, Humans, Female, CD23, IgE, birch pollen allergy, cross-linking
Abstract

Background CD23 mediates IgE-facilitated allergen presentation and subsequent allergen-specific T-cell activation in allergic patients. Objective We sought to investigate key factors regulating IgE-facilitated allergen presentation through CD23 and subsequent T-cell activation. Methods To study T-cell activation by free allergens and different types of IgE–Bet v 1 complexes, we used a molecular model based on monoclonal human Bet v 1–specific IgE, monomeric and oligomeric Bet v 1 allergen, an MHC-matched CD23-expressing B-cell line, and a T-cell line expressing a human Bet v 1–specific T-cell receptor. The ability to cross-link Fcε receptors of complexes consisting of either IgE and monomeric Bet v 1 or IgE and oligomeric Bet v 1 was studied in human FcεRI-expressing basophils. T-cell proliferation by monomeric or oligomeric Bet v 1, which cross-links Fcε receptors to a different extent, was studied in allergic patients’ PBMCs with and without CD23-expressing B cells. Results In our model non–cross-linking IgE–Bet v 1 monomer complexes, as well as cross-linking IgE–Bet v 1 oligomer complexes, induced T-cell activation, which was dependent on the concentration of specific IgE. However, T-cell activation by cross-linking IgE–Bet v 1 oligomer complexes was approximately 125-fold more efficient. Relevant T-cell proliferation occurred in allergic patients’ PBMCs only in the presence of B cells, and its magnitude depended on the ability of IgE–Bet v 1 complexes to cross-link CD23. Conclusion The extent of CD23-mediated T-cell activation depends on the concentration of allergen-specific IgE and the cross-linking ability of IgE-allergen complexes.

Published
2019

80. Underestimation of house dust mite–specific IgE with extract-based ImmunoCAPs compared with molecular ImmunoCAPs

Author

Huang, Huey-Jy, primary, Resch-Marat, Yvonne, additional, Rodriguez-Dominguez, Azahara, additional, Chen, Kuan-Wei, additional, Kiss, Renata, additional, Zieglmayer, Petra, additional, Zieglmayer, René, additional, Lemell, Patrick, additional, Horak, Friedrich, additional, Valenta, Rudolf, additional, and Vrtala, Susanne, additional

Published
2018
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89. Abstracts from the 11th Symposium on Experimental Rhinology and Immunology of the Nose (SERIN 2017)

Author

Gracia, Ibon Eguiluz, primary, Rondón, Carmen, additional, Campo, Paloma, additional, Prieto, Ana, additional, Mayorga, Lina, additional, Galindo, Luisa, additional, Molina, Ana, additional, Blanca, Miguel, additional, Torres, Maria Jose, additional, Morikawa, Taiyo, additional, Fukuoka, Ayumi, additional, Matsushita, Kazufumi, additional, Fujieda, Shigeharu, additional, Yoshimoto, Tomohiro, additional, Iwasaki, Naruhito, additional, Smulders, Tamar, additional, Van Egmond, Danielle, additional, Van Drunen, Kees, additional, Van Der Schee, Marc, additional, Beule, Achim Georg, additional, Berings, Margot, additional, Jult, Anton, additional, Vermeulen, Hanne, additional, De Ruyck, Natalie, additional, Derycke, Lara, additional, Ucar, Hakan, additional, Ghekiere, Philip, additional, Temmerman, Robin, additional, Ellis, John, additional, Bachert, Claus, additional, Lambrecht, Bart, additional, Dullaers, Melissa, additional, Gevaert, Philippe, additional, Arasi, Stefania, additional, Perna, Serena, additional, Resch, Yvonne, additional, Lupinek, Christian, additional, Chen, Kuan-Wei, additional, Vrtala, Susanne, additional, Valenta, Rudolf, additional, Matricardi, Paolo Maria, additional, Gonçalves, Ivânia, additional, Jacinto, Tiago, additional, Amaral, Rita, additional, Pereira, Ana M., additional, Araújo, Luís M., additional, Couto, Mariana, additional, Fonseca, João A., additional, Stjarne, Par, additional, Kaulsay, Ranbir, additional, Pohl, Wolfgang, additional, Plaza, Maria Carmen, additional, Prieto, Ana Maria, additional, Mayorga, Cristobalina, additional, Herknerova, Magdalena, additional, Wang, Tengchin, additional, Wu, Chiejun, additional, Kilimajer, Jonathan, additional, Pujols, Laura, additional, Roca-Ferrer, Jordi, additional, Callejas, Borja, additional, Fuentes-Prado, Mireya, additional, Perez-Gonzalez, Maria, additional, Alobid, Isam, additional, Valero, Antonio, additional, Picado, Cesar, additional, Murray, Ruth, additional, Mullol, Joaquim, additional, Steelant, Brecht, additional, Martens, Katleen, additional, Boeckxstaens, Guy, additional, Seys, Sven F., additional, Hellings, Peter W., additional, Biggs, Timothy C., additional, Hayes, Stephen M., additional, Harries, Philip G., additional, Pender, Sylvia, additional, Salib, Rami J., additional, Kim, Jean, additional, Lee, Hyun Sil, additional, Kalogjera, Livije, additional, Vrkic, Nada, additional, Topic, Anita, additional, Tomljenovic, Dejan, additional, Greguric, Tomislav, additional, Radovanovic, Patricija Bankovic, additional, Jund, Rainer, additional, Haimerl, Pascal, additional, Chaker, Adam M., additional, Schober, Yvonne, additional, Schindela, Sonja, additional, Nockher, Andreas, additional, Schmidt-Weber, Carsten B., additional, Bieren, Julia Esser-Von, additional, Ickrath, Pascal, additional, Kleinsasser, Norbert, additional, Beyersdorf, Niklas, additional, Ding, Xin, additional, Hagen, Rudolf, additional, Hackenberg, Stephan, additional, Cangiano, Daniela, additional, Cinetto, Francesco, additional, Brescia, Giuseppe, additional, Marioni, Gino, additional, Zanotti, Claudia, additional, Schiavon, Franco, additional, Padoan, Roberto, additional, Caputo, Ilaria, additional, Neri, Raffaella, additional, Agostini, Carlo, additional, Kim, Ji Heui, additional, Jang, Yong Ju, additional, Lim, Ji Youn, additional, Kim, Sung Hee, additional, Savlevich, Elena, additional, Gaganov, Leonid, additional, Kochnova, Maria, additional, Egorov, Victor, additional, Fok, Jie Shen, additional, Hanif, Tanzeela, additional, Renkonen, Jutta, additional, Joenväärä, Sakari, additional, Kankainen, Matti, additional, Mäkelä, Mika, additional, Kauppi, Paula, additional, Pelkonen, Anna, additional, Mattila, Pirkko, additional, Renkonen, Risto, additional, Toppila-Salmi, Sanna, additional, Holtappels, Gabriele, additional, Lambrecht, Bart N., additional, Blanca-López, Natalia, additional, Gonzalez-Visiedo, Miguel, additional, Jurado, Raquel, additional, and Canto, Gabriela, additional

Published
2017
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90. Reliable mite-specific IgE testing in nasal secretions by means of allergen microarray

Author

Berings, Margot, primary, Arasi, Stefania, additional, De Ruyck, Natalie, additional, Perna, Serena, additional, Resch, Yvonne, additional, Lupinek, Christian, additional, Chen, Kuan-Wei, additional, Vrtala, Susanne, additional, Pajno, Giovanni Battista, additional, Bachert, Claus, additional, Lambrecht, Bart N., additional, Dullaers, Melissa, additional, Valenta, Rudolf, additional, Matricardi, Paolo Maria, additional, and Gevaert, Philippe, additional

Published
2017
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93. 6th International Symposium on Molecular Allergology (ISMA)

Author

Hilger, Christiane, primary, Swiontek, Kyra, additional, Fischer, Jörg, additional, Hentges, François, additional, Lehners, Christiane, additional, Morisset, Martine, additional, Eberlein, Bernadette, additional, Biedermann, Tilo, additional, Ollert, Markus, additional, Wildner, Sabrina, additional, Stemeseder, Teresa, additional, Freier, Regina, additional, Briza, Peter, additional, Lang, Roland, additional, Batanero, Eva, additional, Villalba, Mayte, additional, Lidholm, Jonas, additional, Hawranek, Thomas, additional, Ferreira, Fatima, additional, Brandstetter, Hans, additional, Gadermaier, Gabriele, additional, Moingeon, Philippe, additional, Groeme, Rachel, additional, Bouley, Julien, additional, Bordas, Véronique, additional, Le Mignon, Maxime, additional, Bussières, Laetitia, additional, Lautrette, Aurélie, additional, Mascarell, Laurent, additional, Lombardi, Vincent, additional, Baron-Bodo, Véronique, additional, Chabre, Henri, additional, Batard, Thierry, additional, Nony, Emmanuel, additional, De Amicis, Karine Marafigo, additional, Watanabe, Alexandra Sayuri, additional, Figo, Daniele Danella, additional, Dos Santos-Pinto, José Roberto Aparecido, additional, Palma, Mario Sergio, additional, Castro, Fabio Fernandes Morato, additional, Kalil, Jorge, additional, Wohlschlager, Therese, additional, Ferreira-Briza, Fatima, additional, Santos, Keity Souza, additional, Faber, Margaretha, additional, Van Gasse, Athina, additional, Sabato, Vito, additional, Hagendorens, Margo M., additional, Bridts, Chris H., additional, De Clerck, Luc S., additional, Perales, Araceli Diaz, additional, Ebo, Didier, additional, Zavadakova, Petra, additional, Buchwalder, Aurélie, additional, Rebeaud, Fabien, additional, Märki, Iwan, additional, Gepp, Barbara, additional, Lengger, Nina, additional, Möbs, Christian, additional, Pfützner, Wolfgang, additional, Radauer, Christian, additional, Bohle, Barbara, additional, Galvao, Clovis Eduardo, additional, Santos-Pinto, Jose Roberto Aparecido, additional, Schwager, Christian, additional, Kull, Skadi, additional, Schocker, Frauke, additional, Behrends, Jochen, additional, Becker, Wolf-Meinhard, additional, Jappe, Uta, additional, Mastrorilli, Carla, additional, Tripodi, Salvatore, additional, Caffarelli, Carlo, additional, Asero, Riccardo, additional, Dondi, Arianna, additional, Ricci, Giampaolo, additional, Dascola, Carlotta Povesi, additional, Calamelli, Elisabetta, additional, Di Rienzo Businco, Andrea, additional, Bianchi, Annamaria, additional, Frediani, Tullio, additional, Verga, Carmen, additional, Iacono, Iride Dello, additional, Peroni, Diego, additional, Pingitore, Giuseppe, additional, Bernardini, Roberto, additional, Matricardi, Paolo Maria, additional, Hofer, Heidi, additional, Asam, Claudia, additional, Hauser, Michael, additional, Himly, Martin, additional, Ebner, Christof, additional, Lemoine, Pierrick, additional, Jain, Karine, additional, Abiteboul, Kathy, additional, Arvidsson, Monica, additional, Rak, Sabina, additional, Mota, Inês, additional, Garcia, Filipe Benito, additional, Gaspar, Angela, additional, Arêde, Cristina, additional, Piedade, Susana, additional, Sampaio, Graça, additional, Pires, Graça, additional, Borrego, Luís Miguel, additional, Santa-Marta, Cristina, additional, Morais-Almeida, Mário, additional, Popescu, Florin-Dan, additional, Vieru, Mariana, additional, Secureanu, Florin-Adrian, additional, Fernandes, Rosa Anita Rodrigues, additional, Carrapatoso, Isabel, additional, Gomes, Raquel, additional, Pereira, Celso, additional, Todo-Bom, Ana, additional, De Basoa, María Cecilia Martín Fernández, additional, Regio, Javier Barrios, additional, De Castro Cordova, Juan, additional, Ferreiro, Antón Fernández, additional, Tsilochristou, Olympia, additional, Perna, Serena, additional, Schwarz, Alina, additional, Rohrbach, Alexander, additional, Cappella, Antonio, additional, Hatzler, Laura, additional, Bauer, Carl-Peter, additional, Hoffmann, Ute, additional, Forster, Johannes, additional, Zepp, Fred, additional, Schuster, Antje, additional, D’amelio, Raffael, additional, Wahn, Ulrich, additional, Keil, Thomas, additional, Lau, Susanne, additional, Apoil, Pol André, additional, Mailhol, Claire, additional, Broué-Chabbert, Anne, additional, Juchet, Agnès, additional, Didier, Alain, additional, Carrer, Elodie, additional, Lanot, Thomas, additional, Blancher, Antoine, additional, Kurtaj, Almedina, additional, Hillebrand, Christoph, additional, Fichtinger, Gerda, additional, Danzer, Martin, additional, Gabriel, Christian, additional, Thalhamer, Theresa, additional, Scheiblhofer, Sandra, additional, Thalhamer, Josef, additional, Weiss, Richard, additional, Wolf, Martin, additional, Pichler, Ulrike, additional, Twaroch, Teresa, additional, Yokoi, Hidenori, additional, Takai, Toshiro, additional, Didierlaurent, Alain, additional, Mari, Adriano, additional, Behrendt, Heidrun, additional, Neubauer, Angela, additional, Stolz, Frank, additional, Ferreira, Fátima, additional, Wallner, Michael, additional, Carvalho, Sara, additional, Lourenço, Tatiana, additional, Cosme, Joana, additional, Duarte, Fátima Cabral, additional, Santos, Amélia Spínola, additional, Costa, Ana Célia, additional, Barbosa, Manuel Pereira, additional, Klinglmayr, Eva, additional, Schweidler, Bettina, additional, Lueftenegger, Lisa, additional, Moser, Stephanie, additional, Doppler, Patrick, additional, Oostingh, Gertie J., additional, Bathke, Arne, additional, Zumbach, Joerg, additional, Panzner, Petr, additional, Vachova, Martina, additional, Vlas, Tomas, additional, Maly, Marek, additional, Posa, Daniela, additional, Hofmaier, Stephanie, additional, Stock, Philippe, additional, Grabenhenrich, Linus, additional, Chen, Kuan-Wei, additional, Resch, Yvonne, additional, Vrtala, Susanne, additional, Valenta, Rudolf, additional, Abramidze, Tamar, additional, Lomidze, Nino, additional, Gotua, Maia, additional, Dapkeviciute, Austeja, additional, Einikyte, Ruta, additional, Norkuniene, Jolita, additional, Skrickiene, Laima, additional, Miskiniene, Asta, additional, Kvedariene, Violeta, additional, Schiener, Maximilian, additional, Moreno-Aguilar, Carmen, additional, Pietsch, Gunilla, additional, Intyre, Mareike Mc, additional, Schwarze, Lea, additional, Rußkamp, Dennis, additional, Spillner, Edzard, additional, Darsow, Ulf, additional, Schmidt-Weber, Carsten, additional, Blank, Simon, additional, Longé, Cyril, additional, Brazdova, Andrea, additional, Brunet, Jean-Louis, additional, Schwartz, Claire, additional, Girodet, Bruno, additional, Lavaud, François, additional, Birnbaum, Joelle, additional, Thi, Nhân Pham, additional, Duchateau, Magalie, additional, Chamot-Rooke, Julia, additional, Guilloux, Laurence, additional, Selva, Marie-Ange, additional, Couderc, Rémy, additional, Sénéchal, Hélène, additional, Sutra, Jean-Pierre, additional, Poncet, Pascal, additional, Augustin, Steffen, additional, Pump, Linda, additional, Wald, Martin, additional, Eichhorn, Thomas, additional, Fischer, Frank, additional, Willers, Christoph, additional, Miehe, Michaela, additional, Plum, Melanie, additional, Wolf, Sara, additional, Jabs, Frederic, additional, Raiber, Tim, additional, Bantleon, Frank, additional, Seismann, Henning, additional, Jakob, Thilo, additional, Apostolovic, Danijela, additional, Tran, Anh Thu, additional, Sanchez-Vidaurre, Sara, additional, Velickovic, Tanja Cirkovic, additional, Starkhammar, Maria, additional, Hamsten, Carl, additional, Van Hage, Marianne, additional, Dubiela, Pawel, additional, Humeniuk, Piotr, additional, Pfeifer, Sabine, additional, Bublin, Merima, additional, Borowski, Tomasz, additional, Hoffmann-Sommergruber, Karin, additional, Verschuren, Martie C. M., additional, Bastiaan-Net, Shanna, additional, Depoortere, Defien, additional, Foetisch, Kay, additional, Scheurer, Stephan, additional, Wichers, Harry J, additional, Noij, Theo, additional, Van Uden, Nikki M.E., additional, Vandenberghe, Karel, additional, Wichers, Harry J., additional, Noij, Theo H. M., additional, Roulias, Anargyros, additional, Parigiani, Maria Alejandra, additional, Ahammer, Linda, additional, Grutsch, Sarina, additional, Tollinger, Martin, additional, Moya, Raquel, additional, López-Matas, Mª Angeles, additional, Reyes, Raquel, additional, Carnés, Jerónimo, additional, Larré, Colette, additional, Rogniaux, Hélène, additional, Lupi, Roberta, additional, Denery-Papini, Sandra, additional, Pablos, Isabel Maria, additional, Eichhorn, Stephanie, additional, Machado, Yoan, additional, Park, Jung-Won, additional, Arora, Naveen, additional, Vieths, Stefan, additional, Tanaka, Charlene, additional, Pineau, Florence, additional, Drouet, Martine, additional, Beaudouin, Etienne, additional, Altenbach, Susan, additional, Mameri, Hamza, additional, Brossard, Chantal, additional, Gaudin, Jean Charles, additional, Moneret-Vautrin, Denise Anne, additional, Paty, Evelyne, additional, Tranquet, Olivier, additional, Masci, Stefania, additional, Moneret-Vautrin, Denise-Anne, additional, Petersen, Arnd, additional, Böttger, Marisa, additional, Rennert, Sandra, additional, Krause, Susanne, additional, Ernst, Martin, additional, Gutsmann, Thomas, additional, Bauer, Johann, additional, Lindner, Buko, additional, Koppelman, Stef, additional, Jayasena, Shyamali, additional, Luykx, Dion, additional, Schepens, Erik, additional, De Jong, Govardus, additional, Isleib, Tom, additional, Nordlee, Julie, additional, Baumert, Joe, additional, Taylor, Steve, additional, Maleki, Soheila, additional, Palladino, Chiara, additional, Sirvent, Sofía, additional, Angelina, Alba, additional, Eiwegger, Thomas, additional, Palomares, Oscar, additional, Breiteneder, Heimo, additional, Claude, Mathilde, additional, Bouchaud, Grégory, additional, Bodinier, Marie, additional, Korte, Robin, additional, Bräcker, Julia, additional, Brockmeyer, Jens, additional, Satoh, Rie, additional, Teshima, Reiko, additional, Tscheppe, Angelika, additional, Palmberger, Dieter, additional, Grabherr, Reingard, additional, Raith, Marianne, additional, Sonnleitner, Linda, additional, Zach, Doris, additional, Woroszylo, Konrad, additional, Focke-Tejkl, Margit, additional, Wank, Herbert, additional, Graf, Thorsten, additional, Kuehn, Annette, additional, Swoboda, Ines, additional, Huber, Sara, additional, Gay-Crosier, Fabienne, additional, Polak, Dominika, additional, Nagl, Birgit, additional, Kitzmüller, Claudia, additional, Samadi, Nazanin, additional, Geyeregger, Rene, additional, Jahn-Schmid, Beatrice, additional, Gomez, Ariel, additional, Haka, Jaana, additional, Hattara, Liisa, additional, Heikkinen, Marika, additional, Niemi, Merja H, additional, Rouvinen, Juha, additional, Saviranta, Petri, additional, Mattila, Pekka, additional, Takkinen, Kristiina, additional, Laukkanen, Marja-Leena, additional, Pablos, Isabel, additional, Kastner, Bianca, additional, Silar, Mira, additional, Selb, Julij, additional, Kogovsek, Rok, additional, Kosnik, Mitja, additional, Korosec, Peter, additional, Pestana, Leticia, additional, Melo, Alcinda Campos, additional, Mendes, Ana, additional, Pedro, Maria Elisa, additional, Santos, Maria Conceição Pereira, additional, Bienvenu, Françoise, additional, Goursaud, Claire, additional, Garnier, Lorna, additional, Jacquenet, Sandrine, additional, Degaud, Michaël, additional, Viel, Sébastien, additional, Barre, Annick, additional, Rougé, Pierre, additional, Bienvenu, Jacques, additional, Vitte, Joana, additional, Bensalah, Amel, additional, Cleach, Isabelle, additional, Mousseau, Laurent, additional, Agabriel, Chantal, additional, Liabeuf, Valérie, additional, Birnbaum, Joëlle, additional, Mège, Jean-Louis, additional, Gardner, James, additional, Gandhi, Minal, additional, Kariyawasam, Harsha, additional, Rotiroti, Giuseppina, additional, Regateiro, Frederico, additional, Faria, Emília, additional, Schmid, Johannes Martin, additional, Dahl, Ronald, additional, Hoffmann, Hans Juergen, additional, Pestana, Letícia, additional, Silva, Diana, additional, Vieira, Teresa, additional, Pereira, Ana Maria, additional, Moreira, André, additional, Delgado, Luís, additional, Prates, Sara, additional, Alves, Cátia, additional, Finelli, Elena, additional, Pinto, Paula Leiria, additional, Cardoso, Bárbara Kong, additional, Cruz, Cíntia, additional, Semedo, Filipa, additional, Tomaz, Elza, additional, Inácio, Filipe, additional, Maity, Santanu, additional, Baricevic-Jones, Ivona, additional, Marsh, Justin T., additional, Johnson, Phil E., additional, Balasundaram, Anuradha, additional, Hope, Anya-May, additional, Taekema, Aafke, additional, Simpson, Angela, additional, Semic-Jusufagic, Aida, additional, Clare Mills, E. N., additional, Nelly, Gourdon Dubois, additional, Laetitia, Sellam, additional, Bruno, Pereira, additional, Elodie, Michaud, additional, Khaled, Messaoudi, additional, Bertrand, Evrard, additional, Jean-Luc, Fauquert, additional, Goodman, Richard E., additional, Plata, Elena Rodríguez, additional, Amaral, Luis, additional, Bartolomé, Borja, additional, Coimbra, Alice, additional, Placido, Jose L, additional, Ganea, Carmen Saviana, additional, Costello, Carol Ann, additional, Sorensen, Martin, additional, Mills, Clare, additional, Rogers, Adrian, additional, Otherhals, Aage, additional, Kalic, Tanja, additional, Ellinger, Isabella, additional, Waltl, Eva, additional, Niederberger-Leppin, Verena, additional, Szczepankiewicz, Dawid, additional, Pruszynska-Oszmalek, Ewa, additional, Skrzypski, Marek, additional, Nowak, Krzysztof W., additional, Szczepankiewicz, Aleksandra, additional, Jang, Gwang-Cheon, additional, Markovic, Iva, additional, Borowski, Andreas, additional, Vetter, Tina, additional, Wohlmann, Andreas, additional, Kuepper, Michael, additional, Friedrich, Karlheinz, additional, Gracia, Ibon Eguiluz, additional, Bosco, Anthony, additional, Dollner, Ralph, additional, Melum, Guro Reinholt, additional, Jones, Anya C, additional, Lexberg, Maria, additional, Holt, Patrick G, additional, Bækkevold, Espen Sønderaal, additional, Jahnsen, Frode Lars, additional, Sobkowiak, Paulina, additional, Rachel, Marta, additional, Narozna, Beata, additional, Jenerowicz, Dorota, additional, Swiatowy, Witold, additional, Breborowicz, Anna, additional, Nestelbacher, Reinhard, additional, and Fukui, Hiroyuki, additional

Published
2016
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94. Journal of Investigative Dermatology / Der p 11 Is a Major Allergen for House Dust Mite-Allergic Patients Suffering from Atopic Dermatitis

Author

Banerjee, Srinita, Resch, Yvonne, Chen, Kuan-Wei, Swoboda, Ines, Focke-Tejkl, Margarete, Blatt, Katharina, Novak, Natalija, Wickmann, Magnus, van Hage, Marianne, Ferrara, Rosetta, Mari, Adriano, Purohit, Ashok, Pauli, Gabrielle, Sibanda, Elopy N., Ndlovu, Portia, Thomas, Wayne R., Krzyzanek, Vladislav, Tacke, Sebastian, Malkus, Ursula, Valent, Peter, Valenta, Rudolf, and Vrtala, Susanne

Published
2015

95. Biochemical, Biophysical and IgE-Epitope Characterization of the Wheat Food Allergen, Tri a 37

Author

Pahr, Sandra, Selb, Regina, Weber, Milena, Focke-Tejkl, Margarete, Hofer, Gerhard, Dordic, Andela, Keller, Walter, Papadopoulos, Nikolaos G., Giavi, Stavroula, Makela, Mika, Pelkonen, Anna, Niederberger, Verena, Vrtala, Susanne, Valenta, Rudolf, Clinicum, and Department of Dermatology, Allergology and Venereology

Subjects
education, Immunology, Molecular Sequence Data, Food Allergies, Crops, DIGESTIBILITY, Biochemistry, Cell Line, Epitopes, MOLECULAR-WEIGHT GLUTENIN, Allergies, Animals, Humans, Amino Acid Sequence, Triticum, Plant Proteins, ALPHA-1-PUROTHIONIN, IDENTIFICATION, GLIADIN, Biology and Life Sciences, Proteins, PEPTIDES, Agriculture, Allergens, Immunoglobulin E, Recombinant Proteins, EXERCISE-INDUCED ANAPHYLAXIS, Allergy Immunotherapy, 3121 General medicine, internal medicine and other clinical medicine, Wheat, Clinical Immunology, Immunotherapy, Rabbits, Epitope Mapping, Food Hypersensitivity, Research Article, Crop Science, Cereal Crops, Antimicrobial Cationic Peptides
Abstract

Wheat is an important staple food and potent allergen source. Recently, we isolated a cDNA coding for wheat alpha-purothionin which is recognized by wheat food allergic patients at risk for severe wheat-induced allergy. The purpose of the present study was the biochemical, biophysical and IgE epitope characterization of recombinant alpha-purothionin. Synthetic genes coding for alpha-purothionin were expressed in a prokaryotic system using Escherichia coli and in a eukaryotic expression system based on baculovirus-infected Sf9-insect cells. Recombinant proteins were purified and characterized by SDS-PAGE, mass spectrometry, circular dichroism, chemical cross-linking and size exclusion chromatography. Five overlapping peptide were synthesized for epitope mapping. Alpha-purothionin-specific rabbit antibodies were raised to perform IgE-inhibition experiments and to study the resistance to digestion. The IgE reactivity of the proteins and peptides from ten wheat food allergic patients was studied in non-denaturing RAST-based binding assays. Alpha-purothionin was expressed in the prokaryotic (EcTri a 37) and in the eukaryotic system (BvTri a 37) as a soluble and monomeric protein. However, circular dichroism analysis revealed that EcTri a 37 was unfolded whereas BvTri a 37 was a folded protein. Both proteins showed comparable IgE-reactivity and the epitope mapping revealed the presence of sequential IgE epitopes in the N-terminal basic thionin domain (peptide1:KSCCRSTLGRNCYNLCRARGAQKLCAGVCR) and in the C-terminal acidic extension domain (peptide3:KGFPKLALESNSDEPDTIEYCNLGCRSSVC, peptide4:CNLGCRSSVCDYMVNAAADDEEMKLYVEN). Natural Tri a 37 was digested under gastric conditions but resistant to duodenal digestion. Immunization with EcTri a 37 induced IgG antibodies which recognized similar epitopes as IgE antibodies from allergic patients and inhibited allergic patients' IgE binding. Reactivity to Tri a 37 does not require a folded protein and the presence of sequential IgE epitopes indicates that sensitization to alpha-purothionin occurs via the gut. Both allergens can be used for in-vitro diagnosis of wheat food allergy. The induction of blocking IgG antibodies suggests the usefulness for immunotherapy.

Published
2014

97. Molecular, Structural and Immunological Characterization of Der p 18, a Chitinase-Like House Dust Mite Allergen

Author

Resch, Yvonne, primary, Blatt, Katharina, additional, Malkus, Ursula, additional, Fercher, Christian, additional, Swoboda, Ines, additional, Focke-Tejkl, Margit, additional, Chen, Kuan-Wei, additional, Seiberler, Susanne, additional, Mittermann, Irene, additional, Lupinek, Christian, additional, Rodriguez-Dominguez, Azahara, additional, Zieglmayer, Petra, additional, Zieglmayer, René, additional, Keller, Walter, additional, Krzyzanek, Vladislav, additional, Valent, Peter, additional, Valenta, Rudolf, additional, and Vrtala, Susanne, additional

Published
2016
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98. Towards a non-allergenic peptide mix containing the T cell epitopes of the clinically most relevant house dust mite allergens for tolerance induction

Author

Huang, Huey-Jy, primary, Curin, Mirela, additional, Banerjee, Srinita, additional, Chen, Kuan-Wei, additional, Garmatiuk, Tetiana, additional, Resch, Yvonne, additional, Campana, Raffaela, additional, Focke-Tejkl, Margarete, additional, Valenta, Rudolf, additional, and Vrtala, Susanne, additional

Published
2016
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