97 results on '"Votino, C"'
Search Results
52. Alendronato a basse dosi e terapia estrogenica sostitutiva nella prevenzione della perdita ossea in donne in postmenopausa chirurgica
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Palomba, S, Sammartino, A, Cerasuolo, Mr, Vassallo, M, Salvatore, B, Ruoto, M, Criscuolo, A, Mandato, V, Votino, C, and Nappi, C
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ormonoterapia ,apparato genitale femminile ,malattie - Published
- 2000
53. OC161: Management and outcome of isolated abdominal calcifications.
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UCL - MD/GYPE - Département de gynécologie, d'obstétrique et de pédiatrie, Votino, C, Lestrade, A, Biard, J M, Debiève, Frédéric, Hubinont, Corinne, Bernard, Pierre, UCL - MD/GYPE - Département de gynécologie, d'obstétrique et de pédiatrie, Votino, C, Lestrade, A, Biard, J M, Debiève, Frédéric, Hubinont, Corinne, and Bernard, Pierre more...
- Published
- 2008
54. OP12.03: Early evaluation of fetal heart anatomy: a prospective study from 6 to 10 weeks' gestation
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Votino, C., primary, Condorelli, M., additional, Votino, R., additional, Bessieres, B., additional, Dessy, H., additional, and Cos, T., additional
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- 2013
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55. P05.01: Correlation between prenatal ultrasound and autopsy findings: a study on first trimester abortion
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Votino, C., primary, Cos, T., additional, Votino, R., additional, Condorelli, M., additional, Segers, V., additional, and Bessieres, B., additional
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- 2013
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56. The impact of prior medical termination of pregnancy on the mother's early relationship with a subsequent infant.
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Alexandre, M., Votino, C., De Noose, L., Cos Sanchez, T., Gaugue, J., and Jani, J.
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ABORTION research , *CHILD development research , *MOTHER-infant relationship , *MOTHER-child relationship , *PERINATAL death , *ABORTION & psychology , *CHILD development , *GRIEF , *INTERVIEWING , *LANGUAGE acquisition , *PSYCHOLOGY of mothers , *CASE-control method - Abstract
Objective: There is insufficient research on the mother’s early relationship with a child born subsequent to a previous medical termination of pregnancy (TOP). This study explores mother-infant interactions following prior TOP and the impact on the infant’s development. Methods: Being an exploratory research comprising 12 mother-infant (6–7 months old) couples, following prior TOP, and five controls, this study uses a descriptive methodology and a qualitative approach. The Greenspan and Lieberman Observation Scale (GLOS) and the Stern’s “R”-Interview were employed to investigate the mother-infant relationship. We used the Brunet-Lézine’s Revised Scales (BL-R) and the Projective Kit for Early Childhood (PKEC) to assess the infant’s development. Grief resolution was taken into account (Perinatal Grief Scale, semi-structured interview). Results: The later the perinatal loss, the less likely children are to express their emotions and respond contingently (GLOS). Their psychomotor (BL-R) and emotional (PKEC) development remains adequate. Unresolved grief is associated with more pronounced disturbances: no dyadic exchange (GLOS), language disruptions (BL-R), and withdrawal from the environment (PKEC). Conclusions: This study suggests that mother-infant interactions following a prior late TOP could undergo disturbances, which do not lead systematically to pathogenic effect on the subsequent child. Nevertheless, unresolved grief could lead to adverse effects. [ABSTRACT FROM PUBLISHER] more...
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- 2016
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57. Acceptance, Reliability and Confidence of Diagnosis of Fetal and Neonatal Virtuopsy Compared With Conventional Autopsy
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Cannie, M., primary, Votino, C., additional, Moerman, P. H., additional, Vanheste, R., additional, Segers, V., additional, Van Berkel, K., additional, Hanssens, M., additional, Kang, X., additional, Cos, T., additional, Kir, M., additional, Balepa, L., additional, Divano, L., additional, Foulon, W., additional, De Mey, J., additional, and Jani, J., additional more...
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- 2012
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58. OP26.01: Post-mortem examination of fetal heart in the first half of the pregnancy: a comparison of high-field MRI at 9.4 T to lower-field MRI and to autopsy
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Votino, C., primary, Jani, J., additional, Verhoye, M., additional, Bessieres, B., additional, Fierens, Y., additional, Segers, V., additional, Cos, T., additional, Foulon, W., additional, and Cannie, M., additional more...
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- 2012
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59. OP12.02: The use of spatio-temporal image correlation (STIC) modality before 15 weeks of gestation in a high risk population for congenital heart diseases
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Votino, C., primary, Cos, T., additional, Abu-Rustum, R. S., additional, Dobrescu, O., additional, Gallo, V., additional, Dahman Saidi, S., additional, Dessy, H., additional, and Jani, J., additional
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- 2012
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60. OC161: Management and outcome of isolated abdominal calcifications
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Votino, C., primary, Lestrade, A., additional, Biard, J. M., additional, Debiève, F., additional, Hubinont, C., additional, and Bernard, P., additional
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- 2008
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61. Vanadate regulates the insulin mitogenic effect by modulating SHP-2 association with insulin receptor substrate 1 in JAr human choriocarcinoma cells
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Bifulco, G., primary, Caruso, M., additional, Carlo, C. Di, additional, Acunzo, G., additional, Votino, C., additional, Pellicano, M., additional, Beguinot, F., additional, and Nappi, C., additional
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- 2003
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62. Acceptance, reliability and confidence of diagnosis of fetal and neonatal virtuopsy compared with conventional autopsy: a prospective study
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Mieke Cannie, Votino, C., Moerman, P., Vanheste, R., Segers, V., Berkel, K., Hanssens, M., Cos, T., Kir, M., Lisa Balepa, Divano, L., Walter Foulon, Johan de Mey, Jani, J., Medical Imaging and Physical Sciences, Clinical Pharmacology and Pharmacotherapy, Obstetrics, and Mother and Child more...
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conventional autopsy ,confidence scale ,postmortem MRI ,magnetic resonance imaging ,virtuopsy - Abstract
Objectives To compare prospectively maternal acceptance of fetal and neonatal virtuopsy with that of conventional autopsy and to determine the confidence with which magnetic resonance (MR) virtuopsy can be used to diagnose normality/abnormality of various fetal anatomical structures. Methods MR and/or computed tomography virtuopsy and conventional autopsy were offered to 96 women (102 fetuses/neonates) following termination of pregnancy (TOP), intrauterine fetal death (IUFD) or neonatal death. Multivariable logistic regression analysis was used to investigate the effect on maternal acceptance of virtuopsy and/or conventional autopsy of the age of the mother, gestational age at TOP or delivery after IUFD, order of pregnancy, parity, religion, type of caregiver obtaining consent and reason for death. When parents consented to both MR virtuopsy and conventional autopsy of fetuses = 20 weeks of gestation or neonates, the confidence with which MR virtuopsy could be used to diagnose normality/abnormality of variousanatomical structures was determined on a scale in which conventional autopsy was considered gold standard. On autopsy we classified fetuses/neonates as having either normal or abnormal anatomical structures; these groups were analyzed separately. At virtuopsy, we indicated confidence of diagnosis of normality/abnormality of every anatomical structure in each of these two groups defined at autopsy, using a scale from 0 (definitely abnormal) to 100 (definitely normal). Results Of the 96 women, 99% (n = 95) consented to virtuopsy and 61.5% (n = 59) to both conventional autopsy and virtuopsy; i.e. 36 (37.5%) consented to virtuopsy alone. Maternal acceptance of conventional autopsy was independently positively related to singleton pregnancy, non-Moslem mother, earlier gestation at TOP or delivery afer IUFD and a maternalfetal medicine specialist obtaining consent. Thirty-three fetuses = 20 weeks of gestation had both conventional autopsy and MR virtuopsy, of which 19 had a full autopsy including the brain. In fetuses with normal anatomical structures at conventional autopsy, MR virtuopsy was associated with high diagnostic confidence (scores > 80) for the brain, skeleton, thoracic organs except the heart, abdominal organs except the pancreas, ureters, bladder and genitals. In fetuses with abnormal anatomical structures at autopsy, MR virtuopsy detected the anomalies with high confidence (scores more...
63. Abstracts of the 26th World Congress on Ultrasound in Obstetrics and Gynecology, Rome, Italy, 24-28 September 2016.
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De Robertis, V., Votino, C., Rembouskos, G., Fanelli, T., and Volpe, P.
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CLEFT lip , *CLEFT palate , *DIAGNOSIS of fetal diseases , *DIAGNOSIS - Abstract
An abstract of the article "The retronasal triangle view and maxillary gap at 11-13 weeks in the detection of cleft lip and palate with or without secondary palate involvement," by V. De Robertis and colleagues is presented. more...
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- 2016
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64. Visual and Hearing Impairment Are Associated With Delirium in Hospitalized Patients: Results of a Multisite Prevalence Study
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Alessandro Morandi, Marco Inzitari, Cristina Udina, Neus Gual, Miriam Mota, Elena Tassistro, Anita Andreano, Antonio Cherubini, Simona Gentile, Enrico Mossello, Alessandra Marengoni, Anna Olivé, Francesc Riba, Domingo Ruiz, Elisabet de Jaime, Giuseppe Bellelli, A. Tarasconi, M. Sella, S. Auriemma, G. Paternò, G. Faggian, C. Lucarelli, N. De Grazia, C. Alberto, A. Margola, L. Porcella, I. Nardiello, E. Chimenti, M. Zeni, A. Giani, S. Famularo, E. Romairone, C. Minaglia, C. Ceccotti, G. Guerra, G. Mantovani, F. Monacelli, T. Candiani, A. Ballestrero, F. Santolini, M. Rosso, V. Bono, S. Sibilla, P. Dal Santo, M. Ceci, P. Barone, T. Schirinzi, A. Formenti, G. Nastasi, G. Isaia, D. Gonella, A. Battuello, S. Casson, D. Calvani, F. Boni, A. Ciaccio, R. Rosa, G. Sanna, S. Manfredini, L. Cortese, M. Rizzo, R. Prestano, A. Greco, M. Lauriola, G. Gelosa, V. Piras, M. Arena, D. Cosenza, A. Bellomo, M. LaMontagna, L. Gabbani, L. Lambertucci, S. Perego, G. Parati, G. Basile, V. Gallina, G. Pilone, C. Giudice, F. De, L. Pietrogrande, B. De, M. Mosca, I. Corazzin, P. Rossi, V. Nunziata, F. D'Amico, A. Grippa, S. Giardini, R. Barucci, A. Cossu, L. Fiorin, M. Distefano, M. Lunardelli, M. Brunori, I. Ruffini, E. Abraham, A. Varutti, E. Fabbro, A. Catalano, G. Martino, D. Leotta, A. Marchet, G. Dell'Aquila, A. Scrimieri, M. Davoli, M. Casella, A. Cartei, G. Polidori, D. Brischetto, S. Motta, R. Saponara, P. Perrone, G. Russo, D. Del, C. Car, T. Pirina, S. Franzoni, A. Cotroneo, F. Ghiggia, G. Volpi, C. Menichetti, M. Bo, A. Panico, P. Calogero, G. Corvalli, M. Mauri, E. Lupia, R. Manfredini, F. Fabbian, A. March, M. Pedrotti, M. Veronesi, E. Strocchi, C. Borghi, A. Bianchetti, A. Crucitti, V. DiFrancesco, G. Fontana, L. Bonanni, F. Barbone, C. Serrati, G. Ballardini, M. Simoncelli, G. Ceschia, C. Scarpa, R. Brugiolo, S. Fusco, T. Ciarambino, C. Biagini, E. Tonon, M. Porta, D. Venuti, M. DelSette, M. Poeta, G. Barbagallo, G. Trovato, A. Delitala, P. Arosio, F. Reggiani, G. Zuliani, B. Ortolani, E. Mussio, A. Girardi, A. Coin, G. Ruotolo, A. Castagna, M. Masina, R. Cimino, A. Pinciaroli, G. Tripodi, U. Cannistrà, F. Cassadonte, M. Vatrano, L. Scaglione, P. Fogliacco, C. Muzzuilini, F. Romano, A. Padovani, L. Rozzini, A. Cagnin, F. Fragiacomo, G. Desideri, E. Liberatore, A. Bruni, G. Orsitto, M. Franco, L. Bonfrate, M. Bonetto, N. Pizio, G. Magnani, G. Cecchetti, A. Longo, V. Bubba, L. Marinan, M. Cotelli, M. Turla, M. Sessa, L. Abruzzi, G. Castoldi, D. LoVetere, C. Musacchio, M. Novello, A. Cavarape, A. Bini, A. Leonardi, F. Seneci, W. Grimaldi, F. Fimognari, V. Bambara, A. Saitta, F. Corica, M. Braga, E. Ettorre, C. Camellini, G. Bellelli, G. Annoni, A. Marengoni, A. Crescenzo, G. Noro, R. Turco, M. Ponzetto, L. Giuseppe, B. Mazzei, G. Maiuri, D. Costaggiu, R. Damato, M. Formilan, G. Patrizia, M. Gallucci, M. Paragona, P. Bini, D. Modica, C. Abati, M. Clerici, I. Barbera, F. NigroImperiale, A. Manni, C. Votino, C. Castiglioni, M. Di, M. Degl'Innocenti, G. Moscatelli, S. Guerini, C. Casini, D. Dini, E. D'Imporzano, S. DeNotariis, F. Bonometti, C. Paolillo, A. Riccardi, A. Tiozzo, M. DiBari, S. Vanni, A. Scarpa, D. Zara, P. Ranieri, M. Alessandro, F. Di, D. Pezzoni, C. Platto, V. D'Ambrosio, C. Ivaldi, P. Milia, F. DeSalvo, C. Solaro, M. Strazzacappa, M. Cazzadori, S. Confente, M. Grasso, E. Troisi, V. Guerini, B. Bernardini, C. Corsini, S. Boffelli, A. Filippi, K. Delpin, B. Faraci, E. Bertoletti, M. Vannucci, F. Tesi, P. Crippa, A. Malighetti, D. Bettini, F. Maltese, G. Abruzzese, D. Cosimo, M. Azzini, M. Colombo, G. Procino, S. Fascendini, F. Barocco, P. Del, A. Mazzone, E. Riva, D. Dell'Acqua, M. Cottino, G. Vezzadini, S. Avanzi, C. Brambilla, S. Orini, F. Sgrilli, A. Mello, L. Lombardi, E. Muti, B. Dijk, S. Fenu, C. Pes, P. Gareri, M. Passamonte, R. Rigo, L. Locusta, L. Caser, G. Rosso, S. Cesarini, R. Cozzi, C. Santini, P. Carbone, I. Cazzaniga, R. Lovati, A. Cantoni, P. Ranzani, D. Barra, G. Pompilio, S. Dimori, S. Cernesi, C. Riccò, F. Piazzolla, E. Capittini, C. Rota, F. Gottardi, L. Merla, A. Barelli, A. Millul, G. De, G. Morrone, M. Bigolari, M. Macchi, F. Zambon, C. Pizzorni, G. DiCasaleto, G. Menculini, M. Marcacci, G. Catanese, D. Sprini, T. DiCasalet, M. Bocci, S. Borga, P. Caironi, C. Cat, E. Cingolani, L. Avalli, G. Greco, G. Citerio, L. Gandini, G. Cornara, R. Lerda, L. Brazzi, F. Simeone, M. Caciorgna, D. Alampi, S. Francesconi, E. Beck, B. Antonini, K. Vettoretto, M. Meggiolaro, E. Garofalo, S. Notaro, R. Varutti, F. Bassi, G. Mistraletti, A. Marino, R. Rona, E. Rondelli, I. Riva, A. Scapigliati, A. Cortegiani, F. Vitale, L. Pistidda, R. D'Andrea, L. Querci, P. Gnesin, M. Todeschini, M. Lugano, G. Castelli, M. Ortolani, A. Cotoia, S. Maggiore, L. DiTizio, R. Graziani, I. Testa, E. Ferretti, C. Castioni, F. Lombardi, R. Caserta, M. Pasqua, S. Simoncini, F. Baccarini, M. Rispoli, F. Grossi, L. Cancelliere, M. Carnelli, F. Puccini, G. Biancofiore, A. Siniscalchi, C. Laici, E. Mossello, M. Torrini, G. Pasetti, S. Palmese, R. Oggioni, V. Mangani, S. Pini, M. Martelli, E. Rigo, F. Zuccalà, A. Cherri, R. Spina, I. Calamai, N. Petrucci, A. Caicedo, F. Ferri, P. Gritti, N. Brienza, R. Fonnesu, M. Dessena, G. Fullin, D. Saggioro, Morandi, A, Inzitari, M, Udina, C, Gual, N, Mota, M, Tassistro, E, Andreano, A, Cherubini, A, Gentile, S, Mossello, E, Marengoni, A, Olivé, A, Riba, F, Ruiz, D, de Jaime, E, Bellelli, G, Alessandro Morandi, Marco Inzitari, Cristina Udina, Neus Gual, Miriam Mota, Elena Tassistro, Anita Andreano, Antonio Cherubini, Simona Gentile, Enrico Mossello, Alessandra Marengoni, Anna Olivé, Francesc Riba, Domingo Ruiz, Elisabet de Jaime, Giuseppe Bellelli, Italian Study Group of Delirium, Claudio Borghi, Morandi, Alessandro, Inzitari, Marco, Udina, Cristina, Gual, Neu, Mota, Miriam, Tassistro, Elena, Andreano, Anita, Cherubini, Antonio, Gentile, Simona, Mossello, Enrico, Marengoni, Alessandra, Olivé, Anna, Riba, Francesc, Ruiz, Domingo, de Jaime, Elisabet, Bellelli, Giuseppe, and A Tarasconi, M Sella, S Auriemma, G Paternò, G Faggian, C Lucarelli, N De Grazia, C Alberto, A Margola, L Porcella, I Nardiello, E Chimenti, M Zeni, A Giani, S Famularo, E Romairone, C Minaglia, C Ceccotti, G Guerra, G Mantovani, F Monacelli, C Minaglia, T Candiani, A Ballestrero, C Minaglia, F Santolini, C Minaglia, M Rosso, V Bono, S Sibilla, P Dal Santo, M Ceci, P Barone, T Schirinzi, A Formenti, G Nastasi, G Isaia, D Gonella, A Battuello, S Casson, D Calvani, F Boni, A Ciaccio, R Rosa, G Sanna, S Manfredini, L Cortese, M Rizzo, R Prestano, A Greco, M Lauriola, G Gelosa, V Piras, M Arena, D Cosenza, A Bellomo, M LaMontagna, L Gabbani, L Lambertucci, S Perego, G Parati, G Basile, V Gallina, G Pilone, C Giudice, F De, L Pietrogrande, B De, M Mosca, I Corazzin, P Rossi, V Nunziata, F D'Amico, A Grippa, S Giardini, R Barucci, A Cossu, L Fiorin, M Arena, M Distefano, M Lunardelli, M Brunori, I Ruffini, E Abraham, A Varutti, E Fabbro, A Catalano, G Martino, D Leotta, A Marchet, G Dell'Aquila, A Scrimieri, M Davoli, M Casella, A Cartei, G Polidori, G Basile, D Brischetto, S Motta, R Saponara, P Perrone, G Russo, D Del, C Car, T Pirina, S Franzoni, A Cotroneo, F Ghiggia, G Volpi, C Menichetti, M Bo, A Panico, P Calogero, G Corvalli, M Mauri, E Lupia, R Manfredini, F Fabbian, A March, M Pedrotti, M Veronesi, E Strocchi, C Borghi, A Bianchetti, A Crucitti, V DiFrancesco, G Fontana, L Bonanni, F Barbone, C Serrati, G Ballardini, M Simoncelli, G Ceschia, C Scarpa, R Brugiolo, S Fusco, T Ciarambino, C Biagini, E Tonon, M Porta, D Venuti, M DelSette, M Poeta, G Barbagallo, G Trovato, A Delitala, P Arosio, F Reggiani, G Zuliani, B Ortolani, E Mussio, A Girardi, A Coin, G Ruotolo, A Castagna, M Masina, R Cimino, A Pinciaroli, G Tripodi, U Cannistrà, F Cassadonte, M Vatrano, L Scaglione, P Fogliacco, C Muzzuilini, F Romano, A Padovani, L Rozzini, A Cagnin, F Fragiacomo, G Desideri, E Liberatore, A Bruni, G Orsitto, M Franco, L Bonfrate, M Bonetto, N Pizio, G Magnani, G Cecchetti, A Longo, V Bubba, L Marinan, M Cotelli, M Turla, M Brunori, M Sessa, L Abruzzi, G Castoldi, D LoVetere, C Musacchio, M Novello, A Cavarape, A Bini, A Leonardi, F Seneci, W Grimaldi, F Seneci, F Fimognari, V Bambara, A Saitta, F Corica, M Braga, E Ettorre, C Camellini, G Bellelli, G Annoni, A Marengoni, A Bruni, A Crescenzo, G Noro, R Turco, M Ponzetto, L Giuseppe, B Mazzei, G Maiuri, D Costaggiu, R Damato, E Fabbro, M Formilan, G Patrizia, M Gallucci, C Minaglia, M Paragona, P Bini, D Modica, C Abati, M Clerici, I Barbera, F NigroImperiale, A Manni, C Votino, C Castiglioni, M Di, M Degl'Innocenti, G Moscatelli, S Guerini, C Casini, D Dini, E D'Imporzano, S DeNotariis, F Bonometti, C Paolillo, A Riccardi, A Tiozzo, A Riccardi, C Paolillo, M DiBari, S Vanni, A Scarpa, D Zara, P Ranieri, M Alessandro, P Calogero, G Corvalli, F Di, D Pezzoni, C Platto, V D'Ambrosio, C Ivaldi, P Milia, F DeSalvo, C Solaro, M Strazzacappa, M Bo, A Panico, M Cazzadori, S Confente, M Bonetto, M Grasso, E Troisi, G Magnani, G Cecchetti, V Guerini, B Bernardini, C Corsini, S Boffelli, A Filippi, K Delpin, B Faraci, E Bertoletti, M Vannucci, F Tesi, P Crippa, A Malighetti, D Bettini, F Maltese, M Formilan, G Abruzzese, C Minaglia, D Cosimo, M Azzini, M Cazzadori, M Colombo, G Procino, S Fascendini, F Barocco, P Del, F D'Amico, A Grippa, A Mazzone, E Riva, D Dell'Acqua, M Cottino, G Vezzadini, S Avanzi, C Brambilla, S Orini, F Sgrilli, A Mello, L Lombardi, E Muti, B Dijk, S Fenu, C Pes, P Gareri, A Castagna, M Passamonte, F De, R Rigo, L Locusta, L Caser, G Rosso, S Cesarini, R Cozzi, C Santini, P Carbone, I Cazzaniga, R Lovati, A Cantoni, P Ranzani, D Barra, G Pompilio, S Dimori, S Cernesi, C Riccò, F Piazzolla, E Capittini, C Rota, F Gottardi, L Merla, A Barelli, A Millul, G De, G Morrone, M Bigolari, C Minaglia, M Macchi, F Zambon, F D'Amico, F D'Amico, C Pizzorni, G DiCasaleto, G Menculini, M Marcacci, G Catanese, D Sprini, T DiCasalet, M Bocci, S Borga, P Caironi, C Cat, E Cingolani, L Avalli, G Greco, G Citerio, L Gandini, G Cornara, R Lerda, L Brazzi, F Simeone, M Caciorgna, D Alampi, S Francesconi, E Beck, B Antonini, K Vettoretto, M Meggiolaro, E Garofalo, A Bruni, S Notaro, R Varutti, F Bassi, G Mistraletti, A Marino, R Rona, E Rondelli, I Riva, A Scapigliati, A Cortegiani, F Vitale, L Pistidda, R D'Andrea, L Querci, P Gnesin, M Todeschini, M Lugano, G Castelli, M Ortolani, A Cotoia, S Maggiore, L DiTizio, R Graziani, I Testa, E Ferretti, C Castioni, F Lombardi, R Caserta, M Pasqua, S Simoncini, F Baccarini, M Rispoli, F Grossi, L Cancelliere, M Carnelli, F Puccini, G Biancofiore, A Siniscalchi, C Laici, E Mossello, M Torrini, G Pasetti, S Palmese, R Oggioni, V Mangani, S Pini, M Martelli, E Rigo, F Zuccalà, A Cherri, R Spina, I Calamai, N Petrucci, A Caicedo, F Ferri, P Gritti, N Brienza, R Fonnesu, M Dessena, G Fullin, D Saggioro more...
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medicine.medical_specialty ,Activities of daily living ,Cross-sectional study ,Hearing loss ,medicine.medical_treatment ,Visual impairment ,Psychological intervention ,visual impairment ,Socio-culturale ,behavioral disciplines and activities ,Hearing impairment, delirium, older, sensory deficits, visual impairment ,sensory deficit ,Hearing impairment ,03 medical and health sciences ,delirium ,older ,sensory deficits ,0302 clinical medicine ,Risk Factors ,Activities of Daily Living ,mental disorders ,medicine ,Humans ,Dementia ,030212 general & internal medicine ,LS4_4 ,Hearing Loss ,General Nursing ,Rehabilitation ,business.industry ,Health Policy ,General Medicine ,medicine.disease ,nervous system diseases ,Cross-Sectional Studies ,Italy ,Emergency medicine ,Delirium ,Geriatrics and Gerontology ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
Objective: Sensory deficits are important risk factors for delirium but have been investigated in single-center studies and single clinical settings. This multicenter study aims to evaluate the association between hearing and visual impairment or bi-sensory impairment (visual and hearing impairment) and delirium. Design: Cross-sectional study nested in the 2017 "Delirium Day" project. Setting and participants: Patients 65 years and older admitted to acute hospital medical wards, emergency departments, rehabilitation wards, nursing homes, and hospices in Italy. Methods: Delirium was assessed with the 4AT (a short tool for delirium assessment) and sensory deficits with a clinical evaluation. We assessed the association between delirium, hearing and visual impairment in multivariable logistic regression models, adjusting for: Model 1, we included predisposing factors for delirium (ie, dementia, weight loss and autonomy in the activities of daily living); Model 2, we added to Model 1 variables, which could be considered precipitating factors for delirium (ie, psychoactive drugs and urinary catheters). Results: A total of 3038 patients were included; delirium prevalence was 25%. Patients with delirium had a higher prevalence of hearing impairment (30.5% vs 18%; P < .001), visual impairment (24.2% vs 15.7%; P < .01) and bi-sensory impairment (16.2% vs 7.5%) compared with those without delirium. In the multivariable logistic regression analysis, the presence of bi-sensory impairment was associated with delirium in Model 1 [odds ratio (OR) 1.5, confidence interval (CI) 1.2-2.1; P = .00] and in Model 2 (OR 1.4; CI 1.1-1.9; P = .02), whereas the presence of visual and hearing impairment alone was not associated with delirium either in Model 1 (OR 0.8; CI 0.6-1.2, P = .36; OR 1.1; CI 0.8-1.4; P = .42) or in Model 2 (OR 0.8, CI 0.6-1.2, P = .27; OR 1.1, CI 0.8-1.4, P = .63). Conclusions and implications: Our findings support the importance of routine screening and specific interventions by a multidisciplinary team to implement optimal management of sensory impairments and hence prevention and the management of the patients with delirium. more...
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- 2021
65. Isolated Upward Rotation of the Fetal Cerebellar Vermis (Blake's Pouch Cyst) Is a Normal Variant: An Analysis of 111 Cases
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Gianluigi Pilu, Elisa Montaguti, C. Votino, Paolo Volpe, Francesco Toni, Monica Maffei, Ginevra Salsi, G. Volpe, Eva Pompilii, T. Fanelli, Salsi G., Volpe G., Montaguti E., Fanelli T., Toni F., Maffei M., Votino C., Pompilii E., Pilu G., and Volpe P. more...
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Prenatal Diagnosi ,Embryology ,medicine.medical_specialty ,Colonic Pouche ,Rotation ,Colonic Pouches ,Prenatal diagnosis ,Ultrasonography, Prenatal ,Cerebellar Vermi ,Posterior fossa ,Interquartile range ,Retrospective Studie ,Pregnancy ,Prenatal Diagnosis ,medicine ,Blake's pouch cyst ,Humans ,Radiology, Nuclear Medicine and imaging ,Cyst ,Retrospective Studies ,Fetus ,medicine.diagnostic_test ,Obstetrics ,business.industry ,Cysts ,Infant, Newborn ,Obstetrics and Gynecology ,Gestational age ,Infant ,Magnetic resonance imaging ,General Medicine ,medicine.disease ,Magnetic Resonance Imaging ,Prenatal magnetic resonance ,Cranial Fossa, Posterior ,Prenatal ultrasound ,Pediatrics, Perinatology and Child Health ,Cerebellar vermis ,Female ,business ,Dandy-Walker Syndrome ,Human ,Cerebellar Vermis - Abstract
Introduction: The objective of the study was to provide more detailed data about fetal isolated upward rotation of the cerebellar vermis rotation (Blake’s pouch cyst) in particular regarding pregnancy outcome. Methods: This is a retrospective study of all cases of fetal isolated upward rotation of the cerebellar vermis (URCV) diagnosed in 3 referral centers in Italy from January 2009 to November 2019. Whenever possible, prenatal magnetic resonance imaging (MRI) was performed and a fetal karyotype was obtained. A detailed follow-up was obtained by consultation of medical records, interview with the parents, and the pediatricians. Results: Our study population included 111 patients with a prenatal diagnosis of isolated URCV made at a median gestational age of 21 weeks +3 days (interquartile range (IQR) 21 + 0–22 + 2). The median brain stem-vermis (BV) angle was 27° (IQR 24–29°). In 37.9% of the cases, a regression of the finding with restoration of normal anatomy was noted at a follow-up scan or at postnatal checks. A BV angle of 25° or less predicted regression with a probability in excess of 90%. MRI was performed in utero or at birth in 101 patients and always confirmed sonographic diagnosis. Fetal CGH array and/or karyotype was available in 97 cases and was always normal, but in 1 case. A postnatal follow-up was available in 102 infants (mean 7 months, range 0–10 years of age) and documented a normal neurologic development in all the cases. Conclusions: Isolated URCV is most likely a normal variant of fetal anatomy without clinical consequences, at least at an early follow-up. A BV angle of 25° or less predicts intrauterine regression of the finding, but the outcome is good in all the cases. When a confident sonographic diagnosis is made, MRI is not mandatory. The risk of a chromosomal anomaly in these cases is probably low. more...
- Published
- 2020
66. Proteomic screening identifies calreticulin as a miR-27a direct target repressing MHC class I cell surface exposure in colorectal cancer
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Marianna Santopaolo, Mario Galgani, Giuseppe Matarese, Alessandra Fucci, Carmelo Laudanna, Tommaso Colangelo, Biagio Pucci, Mario Bigioni, Pamela Ziccardi, Lina Sabatino, Maria Rita Milone, Carolina Votino, Vittorio Colantuoni, Giovanna Polcaro, Massimo Pancione, Carlo Alberto Maggi, Gianluigi Mazzoccoli, Alfredo Budillon, Monica Binaschi, Ada Piepoli, Matteo Fassan, Livio Muccillo, Colangelo, T, Polcaro, G, Ziccardi, ANNA MARIA, Pucci, B, Muccillo, Massimiliano, Galgani, Mario, Fucci, A, Milone, M. R, Budillon, A, Santopaolo, M, Votino, C, Pancione, M, Piepoli, A, Mazzoccoli, G, Binaschi, M, Bigioni, M, Maggi, C. A, Fassan, M, Laudanna, C, Matarese, G, Sabatino, L, and Colantuoni, V. more...
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Proteomics ,0301 basic medicine ,Cancer Research ,Cell ,Apoptosis ,Colorectal Neoplasm ,CD8-Positive T-Lymphocytes ,Mice ,Cytotoxic T cell ,Mice, Inbred BALB C ,Neovascularization, Pathologic ,biology ,MicroRNA ,Up-Regulation ,3. Good health ,medicine.anatomical_structure ,Original Article ,Female ,RNA Interference ,Colorectal Neoplasms ,Human ,Immunology ,Down-Regulation ,Mice, Nude ,Adenocarcinoma ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Cell Line, Tumor ,microRNA ,MHC class I ,medicine ,Animals ,Humans ,Cell Proliferation ,Base Sequence ,Animal ,Cell growth ,Histocompatibility Antigens Class I ,Apoptosi ,Proteomic ,CD8-Positive T-Lymphocyte ,Cell Biology ,HCT116 Cells ,MicroRNAs ,030104 developmental biology ,Perforin ,HCT116 Cell ,biology.protein ,Cancer research ,Calreticulin ,Sequence Alignment ,Proteomic screening ,calreticulin ,miR-27a ,cell ,cancer ,CD8 - Abstract
Impairment of the immune response and aberrant expression of microRNAs are emerging hallmarks of tumour initiation/progression, in addition to driver gene mutations and epigenetic modifications. We performed a preliminary survey of independent adenoma and colorectal cancer (CRC) miRnoma data sets and, among the most dysregulated miRNAs, we selected miR-27a and disclosed that it is already upregulated in adenoma and further increases during the evolution to adenocarcinoma. To identify novel genes and pathways regulated by this miRNA, we employed a differential 2DE-DIGE proteome analysis. We showed that miR-27a modulates a group of proteins involved in MHC class I cell surface exposure and, mechanistically, demonstrated that calreticulin is a miR-27a direct target responsible for most downstream effects in epistasis experiments. In vitro miR-27a affected cell proliferation and angiogenesis; mouse xenografts of human CRC cell lines expressing different miR-27a levels confirmed the protein variations and recapitulated the cell growth and apoptosis effects. In vivo miR-27a inversely correlated with MHC class I molecules and calreticulin expression, CD8+ T cells infiltration and cytotoxic activity (LAMP-1 exposure and perforin release). Tumours with high miR-27a, low calreticulin and CD8+ T cells' infiltration were associated with distant metastasis and poor prognosis. Our data demonstrate that miR-27a acts as an oncomiRNA, represses MHC class I expression through calreticulin downregulation and affects tumour progression. These results may pave the way for better diagnosis, patient stratification and novel therapeutic approaches. more...
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- 2016
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67. UHRF1 coordinates peroxisome proliferator activated receptor gamma (PPARG) epigenetic silencing and mediates colorectal cancer progression
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Alessandra Fucci, Lina Sabatino, Lucia Altucci, Vittorio Colantuoni, Vincenzo Carafa, Massimo Pancione, Angela Nebbioso, Michele Ceccarelli, Carmelo Laudanna, Carolina Votino, Christian Pistore, Federica Babbio, Ian Marc Bonapace, Sabatino, L, Fucci, A, Pancione, M, Carafa, V, Nebbioso, Angela, Pistore, C, Babbio, F, Votino, C, Laudanna, C, Ceccarelli, M, Altucci, Lucia, Bonapace, Im, and Colantuoni, V. more...
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Male ,Cancer Research ,Peroxisome proliferator-activated receptor gamma ,endocrine system diseases ,Ubiquitin-Protein Ligases ,Peroxisome proliferator-activated receptor ,Epigenesis, Genetic ,Cell Movement ,Reference Values ,Cell Line, Tumor ,PPARg ,Genetics ,Gene silencing ,Humans ,Epigenetics ,Gene Silencing ,Intestinal Mucosa ,UHRF1 ,Promoter Regions, Genetic ,Molecular Biology ,Aged ,Neoplasm Staging ,Regulation of gene expression ,chemistry.chemical_classification ,Aged, 80 and over ,colon ,biology ,Reproducibility of Results ,nutritional and metabolic diseases ,DNA Methylation ,Middle Aged ,Prognosis ,Gene Expression Regulation, Neoplastic ,PPAR gamma ,Histone ,DNA demethylation ,chemistry ,colon cancer ,DNA methylation ,Cancer research ,biology.protein ,CCAAT-Enhancer-Binding Proteins ,Disease Progression ,Female ,Colorectal Neoplasms ,epigenetic - Abstract
""Peroxisome proliferator-activated receptor gamma (PPARG) inactivation has been identified as an important step in colorectal cancer (CRC) progression, although the events involved have been partially clarified. UHRF1 is emerging as a cofactor that coordinates the epigenetic silencing of tumor suppressor genes, but its role in CRC remains elusive. Here, we report that UHRF1 negatively regulates PPARG and is associated with a higher proliferative, clonogenic and migration potential. Consistently, UHRF1 ectopic expression induces PPARG repression through its recruitment on the PPARG promoter fostering DNA methylation and histone repressive modifications. In agreement, UHRF1 knockdown elicits PPARG re-activation, accompanied by positive histone marks and DNA demethylation, corroborating its role in PPARG silencing. UHRF1 overexpression, as well as PPARG-silencing, imparts higher growth rate and phenotypic features resembling those occurring in the epithelial-mesenchymal transition. In our series of 110 sporadic CRCs, high UHRF1-expressing tumors are characterized by an undifferentiated phenotype, higher proliferation rate and poor clinical outcome only in advanced stages III-IV. In addition, the inverse relationship with PPARG found in vitro is detected in vivo and UHRF1 prognostic significance appears closely related to PPARG low expression, as remarkably validated in an independent dataset. The results demonstrate that UHRF1 regulates PPARG silencing and both genes appear to be part of a complex regulatory network. These findings suggest that the relationship between UHRF1 and PPARG may have a relevant role in CRC progression.Oncogene advance online publication, 30 January 2012; doi:10.1038\\\/onc.2012.3."" more...
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- 2012
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68. Is uterine artery embolization for cervical ectopic pregnancy always safe?
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Giuseppe Maria Maruotti, A. Agangi, Rosamaria Oppedisano, Mario Quarantelli, L. L. Mazzarelli, Carmela Votino, Vittorio Iaccarino, Pasquale Martinelli, Martinelli, Pasquale, Maruotti, Gm, Oppedisano, R, Agangi, A, Mazzarelli, Ll, Votino, C, Quarantelli, M, and Iaccarino, Vittorio more...
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Adult ,medicine.medical_specialty ,medicine.medical_treatment ,Cervical pregnancy ,Cervix Uteri ,Hysterectomy ,Dilatation and Curettage ,Uterine artery embolization ,Obstetrics and gynaecology ,Pregnancy ,medicine ,Electrocoagulation ,Humans ,Embolization ,Ectopic pregnancy ,Obstetrics ,business.industry ,Uterus ,Obstetrics and Gynecology ,Myoma ,Arteries ,medicine.disease ,Embolization, Therapeutic ,Surgery ,Pregnancy, Ectopic ,Female ,business - Abstract
The study objective was to assess the feasibility and the efficacy of bilateral uterine artery embolization (BUAE) for the treatment of cervical pregnancy. The design was a series of 3 cases of viable cervical pregnancy diagnosed by transvaginal ultrasonography and treated by means of BUAE and subsequent uterine curettage. Three women with viable cervical pregnancy underwent BUAE and subsequent uterine curettage in the department of obstetrics and gynecology, High Risk Pregnancy Center, University "Federico II" of Naples. Measurements included surgical outcomes and preservation of fertility. The treatment was effective in all cases. Two patients resumed normal menstruation about 1 month after the procedure, whereas 1 patient underwent a hysterectomy 2 weeks after embolization because of acute ischemic degeneration of a concomitant myoma. The conservative management of cervical pregnancy with angiographic BUAE is a feasible and effective option, even if subsequent hysterectomy may be required. Counseling is necessary. more...
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- 2007
69. Vanadate regulates the insulin mitogenic effect by modulating SHP-2 association with insulin receptor substrate 1 in JAr human choriocarcinoma cells
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G. Acunzo, Carmine Nappi, Massimiliano Pellicano, C. Di Carlo, C. Votino, Matilde Caruso, Francesco Beguinot, Giuseppe Bifulco, Bifulco, G, Caruso, M, DI CARLO, Costantino, Acunzo, G, Votino, C, Pellicano, M, Beguinot, F, Nappi, Carmine, and Bifulco, Giuseppe more...
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MAPK/ERK pathway ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Placenta ,Protein Tyrosine Phosphatase, Non-Receptor Type 11 ,Protein tyrosine phosphatase ,Carbohydrate metabolism ,Endocrinology ,Pregnancy ,Internal medicine ,medicine ,Tumor Cells, Cultured ,Humans ,Insulin ,Vanadate ,Choriocarcinoma ,Phosphorylation ,Protein kinase A ,biology ,Intracellular Signaling Peptides and Proteins ,Obstetrics and Gynecology ,Phosphoproteins ,IRS1 ,Enzyme Activation ,Insulin receptor ,Glucose ,Uterine Neoplasms ,biology.protein ,Insulin Receptor Substrate Proteins ,Female ,Mitogen-Activated Protein Kinases ,Protein Tyrosine Phosphatases ,Vanadates ,Cell Division - Abstract
Maternal hyperglycemia alters placental glucose metabolism and induces placental hypercellularity. In this study we investigated, in JAr cells, the effect of a protein tyrosine phosphatase inhibitor, vanadate, on the insulin receptor substrate 1 (IRS1)-mitogen-activated protein kinase (MAPK) pathway and on cell proliferation in the presence of normal or high glucose concentration. When JAr cells were cultured in the presence of 25 mmol/l glucose, treatment with vanadate completely prevented SHP-2 association with IRS1. However, vanadate treatment reverted the effect of high glucose on basal and insulin-stimulated insulin receptor and IRS1 phosphorylation. Similar effects were observed on MAPK activation. These events determined a related modification in cell proliferation. Indeed, after high glucose and vanadate treatment, thymidine incorporation levels were comparable to those observed in the presence of normal glucose concentration and in the absence of vanadate. Therefore, in JAr cells, vanadate exerts an inhibitory effect on cell proliferation. This action is related to a modulation of the SHP-2 association with IRS1 that in turn might regulate the phosphorylation state of the main substrates involved in mitogenesic signaling of the insulin receptor. more...
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- 2003
70. Effect of estriol treatment per vaginam before Burch culposuspension]
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S, Palomba, V, Napolitano, A, Sammartino, A, Di Spiezio Sardo, M, Vassallo, V, Mandato, M, Ruoto, G, Acunzo, C, Votino, C, Nappi, Palomba, S, Napolitano, V, Sammartino, A, DI SPIEZIO SARDO, Attilio, Vassallo, M, Mandato, V, Ruoto, M, Acunzo, G, Votino, C, and Nappi, Carmine more...
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Postmenopause ,Administration, Intravaginal ,Urodynamics ,Time Factors ,Estriol ,Urinary Incontinence, Stress ,Vagina ,Humans ,Female ,Prospective Studies ,Middle Aged ,Follow-Up Studies ,Ultrasonography - Abstract
To assess the effect of estriol treatment per vaginam before Burch culposuspension in postmenopausal women with stress urinary incontinence (IUS).prospective randomised study.Department of Gynaecology, Obstetrics and Physiopathology of Human Reproduction-Medical School-University of Naples Federico II .twenty women in postmenopause at least from five years with a urogenital symptomatology due to IUS.women were randomised into one of two groups (treated or control) and they were submitted to an evaluation of vulva and vagina trophism. All the women were submitted to a urodynamic examination and to a transvaginal ultrasonography with evaluation of pubis-bladder neck distance, bladder and proximal urethra position, before treatment, one week before the operation and after six months from the same operation.subjective symptomatology and urodynamic parameters between treated and control groups before and after operation.After 12 weeks of treatment, a significant improvement of subjective symptomatology and a not significant improvement of all the urodynamic parameters in the treated group in comparison with the control group have been demonstrated, while any anatomic alteration compared with the basal hasn t been observed. After six months from the operation in all the women a significant reduction of subjective quantity of urine lost after a strain has been demonstrated and significant variations of urodynamic parameters without significant differences between treated group and control group were also observed.The estriol treatment per vaginam is not so effective on the result of Burch culposuspension in postmenopausal women with IUS. more...
- Published
- 2001
71. Emerging role of the β-catenin-PPARγ axis in the pathogenesis of colorectal cancer
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Angelo Lupo, Vittorio Colantuoni, Massimo Pancione, Antonio Lavecchia, Lina Sabatino, Carolina Votino, Ettore Novellino, Tommaso Colangelo, Sabatino, L, Pancione, M, Votino, C, Colangelo, T, Lupo, A, Novellino, Ettore, Lavecchia, Antonio, and Colantuoni, V. more...
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Genomic instability ,Beta-catenin ,Colorectal cancer ,Peroxisome proliferator-activated receptor ,Antineoplastic Agents ,Biology ,Peroxisome proliferator-activated receptor γ ,medicine.disease_cause ,Peroxisome proliferator activated receptor γ ligand ,medicine ,Wnt/β-catenin pathway ,Animals ,Humans ,Molecular Targeted Therapy ,Topic Highlight ,Epigenetics ,Wnt Signaling Pathway ,beta Catenin ,chemistry.chemical_classification ,Gastroenterology ,Wnt signaling pathway ,General Medicine ,medicine.disease ,PPAR gamma ,Crosstalk (biology) ,chemistry ,Drug Design ,Catenin ,Immunology ,biology.protein ,Cancer research ,Colorectal Neoplasms ,Carcinogenesis - Abstract
Multiple lines of evidence indicate that Wnt/β-catenin signaling plays a fundamental role in colorectal cancer (CRC) initiation and progression. Recent genome-wide data have confirmed that in CRC this pathway is one of the most frequently modified by genetic or epigenetic alterations affecting almost 90% of Wnt/β-catenin gene members. A major challenge is thus learning how the corrupted coordination of this pathway is tied to other signalings to enhance cell growth. Peroxisome proliferator activated receptor γ (PPARγ) is emerging as a growth-limiting and differentiation-promoting factor. In tumorigenesis it exerts a tumor suppressor role and is potentially linked with the Wnt/β-catenin pathway. Based on these results, the identification of new selective PPARγ modulators with inhibitory effects on the Wnt/β-catenin pathway is becoming an interesting perspective. Should, in fact, these molecules display such properties, new research avenues would be opened aimed at developing new molecular targeted drugs. Herein, we review the basic principles and present new hypotheses underlying the crosstalk between Wnt/β-catenin and PPARγ signaling. Furthermore, we discuss the advances in our understanding as to how their altered regulation can culminate in colon cancer and the efforts aimed at designing novel PPARγ agonists endowed with Wnt/β-catenin inhibitory effects to be used as therapeutic and/or preventive agents. more...
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- 2014
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72. Prenatal identification of invasive placentation using ultrasound in women with placenta previa and prior cesarean delivery.
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Califano G, Saccone G, Maria Maruotti G, Bartolini G, Quaresima P, Morelli M, Venturella R, Votino C, Morlando M, Sarno L, Miceli M, Mazzulla R, Collà Ruvolo C, Nazzaro G, Locci M, Guida M, Berghella V, and Bifulco G more...
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- Humans, Female, Pregnancy, Retrospective Studies, Adult, Placentation, Placenta Previa diagnostic imaging, Ultrasonography, Prenatal, Placenta Accreta diagnostic imaging, Cesarean Section
- Abstract
Objective: To evaluate the performance of ultrasound for antenatal identification of invasive placentation in women with placenta previa in the setting of prior cesarean delivery., Study Design: This was a multicenter, retrospective, cohort study. Singleton pregnancies at risk of placenta accreta because of persistent placenta previa in the setting of prior cesarean delivery who delivered at four centers, from January 2010 to May 2020, were included in the study. For this study, pregnancies with diagnosis of accreta, increta, or percreta were considered under the umbrella term of placenta accreta. All women with placenta previa identified in the second trimester had a follow-up ultrasound at 32-34 weeks. Only those with prior cesarean delivery were considered at risk of placenta accreta. Women were considered with suspected accreta in case of suspected prenatal ultrasound. Women with suspected placenta accreta had delivery planned via cesarean hysterectomy at 34+0 - 35+6 weeks, without any attempt to remove the placenta. The primary endpoint of the study was the performance of ultrasound for antenatal identification of invasive placentation. The following ultrasound signs were evaluated: placenta lacunae; loss of clear space; increased vascularity between myometrium and placenta; intracervical lake; rail sign; uterovesical hypervascularity; increased vascularity in the inferior part of the lower uterine segment potentially extending into the parametrial region; and disruption of bladder-myometrial interface., Results: 180 singleton pregnancies with placenta previa in the setting of prior cesarean delivery were identified. Of them, 155 (86.1%) had antenatal suspected placenta accreta based on ultrasound, having at least one sign of invasive placentation. Of the 155 suspected cases, 99 had confirmed placenta accreta at the time of delivery. Among the 99 cases of confirmed placenta accreta, all of them had at least one sign of invasive placentation at ultrasound. Among the 81 cases with placenta previa, prior cesarean delivery, without placenta accreta, 25/81 (30.9%) had ultrasound scan negative for sign of invasive placentation, and 56/81 (69.1%) had at least one sign of invasive placentation). In particular, 12/81 (14.8%) had placenta lacunae, 16/81 (19.8%) had loss of clear space, 20/81 (24.7%) had increased vascularity between myometrium and placenta, 9/81 (11.1%) had intracervical lake, 14/81 (17.3%) had rail sign, 14 (17.3%) had uterovesical hypervascularity, 5/81 (6.2%) had increased vascularity in the inferior part of the lower uterine segment potentially extending into the parametrial region, 8/81 (9.9%) had disruption of bladder-myometrial interface. In the group of women with confirmed placenta accreta, the most common sign recorded was the disruption of bladder-myometrial interface, being recorded in 88/99 women. Disruption of bladder-myometrial interface had the highest sensitivity in detection placenta accreta. Women with disruption of bladder-myometrial interface at ultrasound had 73-fold increase in the risk of placenta accreta compared to those who did not., Conclusion: Prenatal ultrasound has an excellent diagnostic accuracy in identifying invasive placentation in women with placenta previa and prior cesarean delivery., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.) more...
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- 2024
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73. Correction: Post-liquefaction normospermic human semen behaves as a weak-gel viscoelastic fluid.
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Tomaiuolo G, Fellico F, Preziosi V, Cariati F, Strina I, Votino C, Zullo F, Longobardi S, and Guido S
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Correction for 'Post-liquefaction normospermic human semen behaves as a weak-gel viscoelastic fluid' by Giovanna Tomaiuolo et al. , Soft Matter , 2023, https://doi.org/10.1039/d3sm00443k.
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- 2023
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74. Post-liquefaction normospermic human semen behaves as a weak-gel viscoelastic fluid.
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Tomaiuolo G, Fellico F, Preziosi V, Cariati F, Strina I, Votino C, Zullo F, Longobardi S, and Guido S
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- Humans, Rheology, Semen, Body Fluids
- Abstract
The rheological behavior of human semen is overlooked and essentially unexplored in the scientific literature. Here, we provide the first quantitative experimental evidence that post-liquafaction normospermic human semen behaves as a viscoelastic fluid and the shear moduli can be scaled according to the weak-gel model. more...
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- 2023
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75. Corrigendum to "The Italian guidelines on ultrasound in obstetrics and gynecology: Executive summary of recommendations for practice" [Eur. J. Obstetrics Gynecol. 279 (2022) 176-182].
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Aprile A, Calì G, Chianchiano N, Chiappa V, Corbella P, D'Addario V, Dall'Asta A, De Robertis V, Exacustos C, Familiari A, Fichera A, Formigoni C, Frusca T, Ghi T, Guerriero S, Iuculano A, Labate F, Martinelli P, Monni G, Morlano M, Nonino F, Olivieri C, Paladini D, Peddes C, Prefumo F, Rizzo G, Rustico M, Sarno L, Sciacovelli I, Sciarrone A, Stampalija T, Taddei F, Todros T, Valensise H, Vergani P, Volpe N, Volpe P, Votino C, Bettoncelli G, Bracalente G, Collini Ceccatelli M, Costantini M, D'Aloia A, Ferrazzi E, Giorlandino C, Locci M, Verrotti di Pianella C, Viora E, Zoia R, Bilardo K, and Vicar M more...
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- 2023
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76. A Novel Splicing Variant of COL2A1 in a Fetus with Achondrogenesis Type II: Interpretation of Pathogenicity of In-Frame Deletions.
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Bruni V, Spoleti CB, La Barbera A, Dattilo V, Colao E, Votino C, Bellacchio E, Perrotti N, Giglio S, and Iuliano R
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- Abortion, Eugenic, Achondroplasia diagnosis, Achondroplasia pathology, Achondroplasia surgery, Adult, Alternative Splicing genetics, Female, Fetal Diseases diagnosis, Fetal Diseases pathology, Fetal Diseases surgery, Humans, Imaging, Three-Dimensional, Italy, Mutation, Pregnancy, Protein Isoforms genetics, Sequence Deletion, Ultrasonography, Prenatal, Achondroplasia genetics, Collagen Type II genetics, Fetal Diseases genetics
- Abstract
Achondrogenesis type II (ACG2) is a lethal skeletal dysplasia caused by dominant pathogenic variants in COL2A1 . Most of the variants found in patients with ACG2 affect the glycine residue included in the Gly-X-Y tripeptide repeat that characterizes the type II collagen helix. In this study, we reported a case of a novel splicing variant of COL2A1 in a fetus with ACG2. An NGS analysis of fetal DNA revealed a heterozygous variant c.1267-2_1269del located in intron 20/exon 21. The variant occurred de novo since it was not detected in DNA from the blood samples of parents. We generated an appropriate minigene construct to study the effect of the variant detected. The minigene expression resulted in the synthesis of a COL2A1 messenger RNA lacking exon 21, which generated a predicted in-frame deleted protein. Usually, in-frame deletion variants of COL2A1 cause a phenotype such as Kniest dysplasia, which is milder than ACG2. Therefore, we propose that the size and position of an in-frame deletion in COL2A1 may be relevant in determining the phenotype of skeletal dysplasia. more...
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- 2021
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77. Isolated Upward Rotation of the Fetal Cerebellar Vermis (Blake's Pouch Cyst) Is a Normal Variant: An Analysis of 111 Cases.
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Salsi G, Volpe G, Montaguti E, Fanelli T, Toni F, Maffei M, Votino C, Pompilii E, Pilu G, and Volpe P
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- Cranial Fossa, Posterior diagnostic imaging, Female, Humans, Infant, Infant, Newborn, Magnetic Resonance Imaging, Pregnancy, Prenatal Diagnosis, Retrospective Studies, Rotation, Ultrasonography, Prenatal, Cerebellar Vermis diagnostic imaging, Colonic Pouches, Cysts, Dandy-Walker Syndrome
- Abstract
Introduction: The objective of the study was to provide more detailed data about fetal isolated upward rotation of the cerebellar vermis rotation (Blake's pouch cyst) in particular regarding pregnancy outcome., Methods: This is a retrospective study of all cases of fetal isolated upward rotation of the cerebellar vermis (URCV) diagnosed in 3 referral centers in Italy from January 2009 to November 2019. Whenever possible, prenatal magnetic resonance imaging (MRI) was performed and a fetal karyotype was obtained. A detailed follow-up was obtained by consultation of medical records, interview with the parents, and the pediatricians., Results: Our study population included 111 patients with a prenatal diagnosis of isolated URCV made at a median gestational age of 21 weeks +3 days (interquartile range (IQR) 21 + 0-22 + 2). The median brain stem-vermis (BV) angle was 27° (IQR 24-29°). In 37.9% of the cases, a regression of the finding with restoration of normal anatomy was noted at a follow-up scan or at postnatal checks. A BV angle of 25° or less predicted regression with a probability in excess of 90%. MRI was performed in utero or at birth in 101 patients and always confirmed sonographic diagnosis. Fetal CGH array and/or karyotype was available in 97 cases and was always normal, but in 1 case. A postnatal follow-up was available in 102 infants (mean 7 months, range 0-10 years of age) and documented a normal neurologic development in all the cases., Conclusions: Isolated URCV is most likely a normal variant of fetal anatomy without clinical consequences, at least at an early follow-up. A BV angle of 25° or less predicts intrauterine regression of the finding, but the outcome is good in all the cases. When a confident sonographic diagnosis is made, MRI is not mandatory. The risk of a chromosomal anomaly in these cases is probably low., (© 2021 S. Karger AG, Basel.) more...
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- 2021
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78. Prospective detection and differential diagnosis of cystic posterior fossa anomalies by assessing posterior brain at 11-14 weeks.
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Volpe P, Persico N, Fanelli T, De Robertis V, D'Alessandro J, Boito S, Pilu G, and Votino C
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- Dandy-Walker Syndrome diagnosis, Diagnosis, Differential, Female, Humans, Pregnancy, Pregnancy Trimester, First, Prospective Studies, Brain diagnostic imaging, Cranial Fossa, Posterior diagnostic imaging
- Abstract
Background: The role of the first-trimester scan has expanded from aneuploidy screening to the diagnosis of fetal malformations. Abnormal appearance of the posterior brain at 11-14 weeks gestation is a marker of cerebral anomalies; in fact an increased amount of fluid, particularly when the choroid plexus of the fourth ventricle is not visible and only 2 brain spaces instead of 3 are seen, may indicate the presence of cystic or cyst-like posterior fossa anomalies, such as Blake's pouch cyst or Dandy-Walker malformation., Objective: The purpose of this study was to assess the role of ultrasound scanning in the identification of cystic posterior fossa anomalies at 11-14 weeks gestation., Study Design: A prospective cohort study of fetuses with cystic appearance of the posterior fossa at 11-14 weeks gestation was performed. In all cases and in a control group of 40 normal fetuses, the brainstem-tentorium angle was also measured. The presence or absence of cystic posterior anomalies was determined at birth or at postmortem evaluation., Results: In the period 2014-2018, 32 fetuses with an increased brainstem-occipital bone distance and/or failure to visualize the choroid plexus of fourth ventricle (2 brain spaces) were seen. Of these, 18 fetuses were terminated in the first trimester because of associated anomalies and were excluded from the study because of unavailable autoptic findings. The remaining 14 fetuses eventually were found to have a Dandy-Walker malformation in 4 cases, a Blake's pouch cyst in 8 cases, and normal brain anatomy in 2 cases. Two brain spaces were seen in all cases with Dandy-Walker malformation and in 2 of 8 cases with Blake's pouch cyst. Both brainstem-occipital bone measurement and brainstem-tentorium angle were significantly different in fetuses with Dandy-Walker malformation, Blake's pouch cyst, and control subjects (P<.0001). The brainstem-occipital bone z-scores of fetuses with Dandy-Walker malformation and Blake's pouch cyst were always +3 or more and +1.7 or more, respectively. The brainstem-tentorium angle z-scores were always -5 or less and -0.1 or less, respectively., Conclusion: Our study confirms that sonography of the posterior brain at 11-14 weeks gestation allows the identification of cystic posterior fossa anomalies. A large brainstem-occipital bone predicts Dandy-Walker malformation or Blake's pouch cyst. The presence of 2 brain spaces and a small brainstem-tentorium angle are correlated significantly with the presence of Dandy-Walker malformation., (Copyright © 2019 Elsevier Inc. All rights reserved.) more...
- Published
- 2019
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79. Cleft Palate with or without Cleft Lip: The Role of Retronasal Triangle View and Maxillary Gap at 11-14 Weeks.
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De Robertis V, Rembouskos G, Fanelli T, Votino C, and Volpe P
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- Anatomic Landmarks, Female, Humans, Observer Variation, Predictive Value of Tests, Pregnancy, Pregnancy Trimester, First, Reproducibility of Results, Retrospective Studies, Cleft Lip diagnostic imaging, Cleft Palate diagnostic imaging, Ultrasonography, Prenatal
- Abstract
Objective: To evaluate the presence of maxillary gap (MG) and abnormal retronasal triangle (RT) as markers of cleft palate (CP) with and without cleft lip in the first trimester and to assess their association with the type of orofacial cleft (OC)., Methods: The RT and the mid-sagittal view of the face were evaluated retrospectively by two operators in 26 fetuses with OC and in 80 normal controls to detect abnormal RT and/or MG. The agreement between operators was calculated., Results: Amongst the 26 fetuses, there were 15 cases of bilateral, 6 cases of unilateral, and 4 cases of median cleft lip and palate, and 1 case of CP alone. The MG was observed in 18 cases by operator 1 and in 17 cases by operator 2; an abnormal RT was detected in 21 cases by operator 1 and in 22 cases by operator 2. Great agreement between operators was obtained. In controls, MG or abnormal RT was suspected in 6 and 2-4% of cases, respectively., Conclusions: RT seems to be more sensitive compared to MG; however, the latter showed an additional diagnostic ability when the secondary palate was involved. Both approaches in combination could be useful in detecting OC in the first trimester., (© 2019 S. Karger AG, Basel.) more...
- Published
- 2019
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80. Cervical pessary for preventing preterm birth in twin pregnancies with short cervical length: a systematic review and meta-analysis.
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Saccone G, Ciardulli A, Xodo S, Dugoff L, Ludmir J, D'Antonio F, Boito S, Olearo E, Votino C, Maruotti GM, Rizzo G, Martinelli P, and Berghella V
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- Cervical Length Measurement, Female, Humans, Infant, Newborn, Organ Size, Pregnancy, Randomized Controlled Trials as Topic, Cervix Uteri pathology, Pessaries, Pregnancy, Twin, Premature Birth prevention & control
- Abstract
Objective: To evaluate the effectiveness of cervical pessary for preventing spontaneous preterm birth (SPTB) in twin pregnancies with an asymptomatic transvaginal ultrasound cervical length (TVU CL) in the second trimester., Methods: We performed a meta-analysis including all randomized clinical trials (RCTs) comparing the use of cervical pessary (i.e. intervention group) with expectant management (i.e. control group). The primary outcome was incidence of SPTB <34 weeks., Results: Three trials, including 481 twin pregnancies with short cervix, were analyzed. Two RCTs defined short cervix as TVU CL ≤25 mm and one as TVU CL ≤38 mm. Pessary was not associated with prevention of SPTB, and the mean gestational age at delivery and the mean latency were similar in the pessary group compared to the control group. Moreover, no benefits were noticed in neonatal outcomes., Conclusions: Use of the Arabin pessary in twin pregnancies with short TVU CL at 16-24 weeks does not prevent SPTB or improve perinatal outcome. more...
- Published
- 2017
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81. Aberrant BLM cytoplasmic expression associates with DNA damage stress and hypersensitivity to DNA-damaging agents in colorectal cancer.
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Votino C, Laudanna C, Parcesepe P, Giordano G, Remo A, Manfrin E, and Pancione M
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- Ataxia Telangiectasia genetics, Bloom Syndrome complications, Bloom Syndrome genetics, Bloom Syndrome metabolism, Cell Line, Tumor, Colorectal Neoplasms etiology, Colorectal Neoplasms metabolism, Colorectal Neoplasms pathology, CpG Islands genetics, Cytoplasm metabolism, DNA Methylation, DNA Repair, DNA, Neoplasm genetics, Female, Gene Expression Profiling methods, Gene Silencing, Humans, Male, Neoplasm Proteins genetics, Neoplasm Proteins metabolism, Neoplasm Staging, RNA, Messenger genetics, RecQ Helicases metabolism, Signal Transduction genetics, Up-Regulation, Colorectal Neoplasms genetics, DNA Damage, RecQ Helicases genetics
- Abstract
Background: Bloom syndrome is a rare and recessive disorder characterized by loss-of-function mutations of the BLM gene, which encodes a RecQ 3'-5' DNA helicase. Despite its putative tumor suppressor function, the contribution of BLM to human sporadic colorectal cancer (CRC) remains poorly understood., Methods: The transcriptional regulation mechanism underlying BLM and related DNA damage response regulation in independent CRC subsets and a panel of derived cell lines was investigated by bioinformatics analysis, the transcriptomic profile, a CpG island promoter methylation assay, Western blot, and an immunolocalization assay., Results: In silico analysis of gene expression data sets revealed that BLM is overexpressed in poorly differentiated CRC and exhibits a close connection with shorter relapse-free survival even after adjustment for prognostic factors and pathways that respond to DNA damage response through ataxia telangiectasia mutated (ATM) signaling. Functional characterization demonstrated that CpG island promoter hypomethylation increases BLM expression and associates with cytoplasmic BLM mislocalization and increased DNA damage response both in clinical CRC samples and in derived cancer cell lines. The DNA-damaging agent S-adenosylmethionine suppresses BLM expression, leading to the inhibition of cell growth following accumulation of DNA damage. In tumor specimens, cytoplasmic accumulation of BLM correlates with DNA damage and γH2AX and phosphorylated ATM foci and predicts long-term progression-free survival in metastatic patients treated with irinotecan., Conclusions: Taken together, the findings of this study provide the first evidence that cancer-linked DNA hypomethylation and cytosolic BLM mislocalization might reflect compromised levels of DNA-repair activity and enhanced hypersensitivity to DNA-damaging agents in CRC patients. more...
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- 2017
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82. Knowledge of Human Cytomegalovirus Infection and Prevention in Pregnant Women: A Baseline, Operational Survey.
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Mazzitelli M, Micieli M, Votino C, Visconti F, Quaresima P, Strazzulla A, Torti C, and Zullo F
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- Adult, Cytomegalovirus Infections virology, Female, Humans, Italy epidemiology, Pregnancy, Pregnancy Complications, Infectious virology, Surveys and Questionnaires, Cytomegalovirus isolation & purification, Cytomegalovirus Infections prevention & control, Health Knowledge, Attitudes, Practice, Pregnancy Complications, Infectious prevention & control
- Abstract
Currently, the only efficient way to prevent human Cytomegalovirus (HCMV) infection in pregnancy is primary prophylaxis through hygienic measures. So, we evaluated knowledge of HCMV and its prevention in a group of pregnant women. An anonymous questionnaire with multiple-choice answers was administered to all pregnant women who were followed up at the Obstetrics and Gynecology Unit of "Pugliese-Ciaccio Hospital," a third-level hospital in Catanzaro (Southern Italy), from November 2015 to March 2016. Previously prescribed serology results for HCMV were also evaluated. Three hundred and fifty women participated in the study and the results clearly demonstrated that knowledge of pregnant women about HCMV is poor. Moreover, prescribed screening procedures need to be optimized, since one out of three pregnant women has not been tested for HCMV or the screening was not performed adequately. For this reason, it is important to implement informative campaign in both pregnant women and providing physicians. more...
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- 2017
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83. A case report of Schistosoma haematobium infection in a pregnant migrant raises concerns about lack of screening policies.
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Mazzitelli M, Matera G, Votino C, Visconti F, Strazzulla A, Loria MT, Peronace C, Settembre P, Giancotti A, Liberto MC, Focà A, Zullo F, and Torti C
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- Adult, Animals, Anthelmintics administration & dosage, Female, Humans, Male, Praziquantel administration & dosage, Pregnancy, Schistosomiasis haematobia drug therapy, Young Adult, Pregnancy Complications, Parasitic drug therapy, Schistosoma haematobium isolation & purification, Schistosomiasis haematobia parasitology, Transients and Migrants
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Pregnant women with urinary schistosomiasis should be treated, but screening is not implemented in migrants. We report herein a case of a migrant diagnosed late into pregnancy, after diagnosis was made in her husband. Praziquantel was safe and effective. Schistosomiasis should be considered in pregnant women from endemic countries., (© International Society of Travel Medicine, 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.) more...
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- 2016
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84. The antiproliferative and proapoptotic effects of cladosporols A and B are related to their different binding mode as PPARγ ligands.
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Zurlo D, Ziccardi P, Votino C, Colangelo T, Cerchia C, Dal Piaz F, Dallavalle S, Moricca S, Novellino E, Lavecchia A, Colantuoni V, and Lupo A
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- Anilides pharmacology, Cell Line, Tumor, Cell Proliferation drug effects, Cyclin-Dependent Kinase Inhibitor p21 metabolism, Drug Partial Agonism, G1 Phase Cell Cycle Checkpoints, HEK293 Cells, Humans, Ligands, Molecular Docking Simulation, Oxidative Stress, PPAR gamma antagonists & inhibitors, PPAR gamma genetics, PPAR gamma metabolism, Protein Binding, Protein Conformation, Stereoisomerism, Structure-Activity Relationship, Transcriptional Activation, Antineoplastic Agents pharmacology, Apoptosis drug effects, Naphthols pharmacology, PPAR gamma agonists
- Abstract
Cladosporols are secondary metabolites from Cladosporium tenuissimum characterized for their ability to control cell proliferation. We previously showed that cladosporol A inhibits proliferation of human colon cancer cells through a PPARγ-mediated modulation of gene expression. In this work, we investigated cladosporol B, an oxidate form of cladosporol A, and demonstrate that it is more efficient in inhibiting HT-29 cell proliferation due to a robust G0/G1-phase arrest and p21(waf1/cip1) overexpression. Cladosporol B acts as a PPARγ partial agonist with lower affinity and reduced transactivation potential in transient transfections as compared to the full agonists cladosporol A and rosiglitazone. Site-specific PPARγ mutants and surface plasmon resonance (SPR) experiments confirm these conclusions. Cladosporol B in addition displays a sustained proapoptotic activity also validated by p21(waf1/cip1) expression analysis in the presence of the selective PPARγ inhibitor GW9662. In the DMSO/H2O system, cladosporols A and B are unstable and convert to the ring-opened compounds 2A and 2B. Finally, docking experiments provide the structural basis for full and partial PPARγ agonism of 2A and 2B, respectively. In summary, we report here, for the first time, the structural characteristics of the binding of cladosporols, two natural molecules, to PPARγ. The binding of compound 2B is endowed with a lower transactivation potential, higher antiproliferative and proapoptotic activity than the two full agonists as compound 2A and rosiglitazone (RGZ)., (Copyright © 2016 Elsevier Inc. All rights reserved.) more...
- Published
- 2016
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85. Increased TGF-β: a drawback of tracheal occlusion in human and experimental congenital diaphragmatic hernia?
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Vuckovic A, Herber-Jonat S, Flemmer AW, Ruehl IM, Votino C, Segers V, Benachi A, Martinovic J, Nowakowska D, Dzieniecka M, and Jani JC
- Subjects
- Animals, Fetus metabolism, Humans, Pulmonary Alveoli metabolism, Rabbits, Respiration, Artificial methods, Trachea metabolism, rho-Associated Kinases metabolism, Airway Obstruction metabolism, Hernias, Diaphragmatic, Congenital genetics, Hernias, Diaphragmatic, Congenital metabolism, Lung metabolism, Transforming Growth Factor beta metabolism
- Abstract
Survivors of severe congenital diaphragmatic hernia (CDH) present significant respiratory morbidity despite lung growth induced by fetal tracheal occlusion (TO). We hypothesized that the underlying mechanisms would involve changes in lung extracellular matrix and dysregulated transforming growth factor (TGF)-β pathway, a key player in lung development and repair. Pulmonary expression of TGF-β signaling components, downstream effectors, and extracellular matrix targets were evaluated in CDH neonates who died between birth and the first few weeks of life after prenatal conservative management or TO, and in rabbit pups that were prenatally randomized for surgical CDH and TO vs. sham operation. Before tissue harvesting, lung tissue mechanics in rabbits was measured using the constant-phase model during the first 30 min of life. Human CDH and control fetal lungs were also collected from midterm onwards. Human and experimental CDH did not affect TGF-β/Smad2/3 expression and activity. In human and rabbit CDH lungs, TO upregulated TGF-β transcripts. Analysis of downstream pathways indicated increased Rho-associated kinases to the detriment of Smad2/3 activation. After TO, subtle accumulation of collagen and α-smooth muscle actin within alveolar walls was detected in rabbit pups and human CDH lungs with short-term mechanical ventilation. Despite TO-induced lung growth, mediocre lung tissue mechanics in the rabbit model was associated with increased transcription of extracellular matrix components. These results suggest that prenatal TO increases TGF-β/Rho kinase pathway, myofibroblast differentiation, and matrix deposition in neonatal rabbit and human CDH lungs. Whether this might influence postnatal development of sustainably ventilated lungs remains to be determined., (Copyright © 2016 the American Physiological Society.) more...
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- 2016
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86. Role of CGH array in the diagnosis of autosomal recessive disease: a case of Ellis-van Creveld syndrome.
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D'Ambrosio V, Votino C, Cos T, Boulanger S, Dheedene A, Jani J, and Keymolen K
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- Adult, Base Sequence, Chromosome Disorders diagnosis, Chromosome Disorders genetics, Ellis-Van Creveld Syndrome genetics, Female, Humans, Intercellular Signaling Peptides and Proteins, Male, Membrane Proteins, Molecular Sequence Data, Pregnancy, Comparative Genomic Hybridization, Ellis-Van Creveld Syndrome diagnosis, Genes, Recessive, Prenatal Diagnosis methods, Proteins genetics
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- 2015
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87. Stomach position versus liver-to-thoracic volume ratio in left-sided congenital diaphragmatic hernia.
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Cordier AG, Cannie MM, Guilbaud L, De Laveaucoupet J, Martinovic J, Nowakowska D, Milejska-Lewandowska M, Rodó C, Viaris de Lesegno B, Votino C, Senat MV, Jani JC, and Benachi A
- Subjects
- Female, Fetus pathology, Gestational Age, Hernias, Diaphragmatic, Congenital classification, Hernias, Diaphragmatic, Congenital diagnostic imaging, Humans, Liver diagnostic imaging, Liver embryology, Lung diagnostic imaging, Lung embryology, Lung pathology, Magnetic Resonance Imaging, Organ Size, Pregnancy, Retrospective Studies, Stomach diagnostic imaging, Stomach embryology, Thoracic Cavity diagnostic imaging, Thoracic Cavity embryology, Ultrasonography, Prenatal, Hernias, Diaphragmatic, Congenital pathology, Liver pathology, Prenatal Diagnosis methods, Stomach pathology, Thoracic Cavity pathology
- Abstract
Objective: To describe a new grading method for stomach position (SP) in fetuses with left-sided congenital diaphragmatic hernia (L-CDH) using ultrasound and to correlate SP to liver position and to liver-to-thoracic cavity volume ratio (LiTR) using magnetic resonance imaging., Methods: SP were graded at the level of the 4-chamber view as following: grade 1-to-4 for stomach not visualised, visualised anteriorly at the apex of the heart, stomach showing abdominal structures anteriorly and stomach with its larger part posterior to the level of the atrial-ventricular heart valves, respectively. The LiTR was calculated and correlated to SP using the Mann-Whitney U test., Results: Seventy-four fetuses were included. Median LiTR for grade 1 SP was 0% and was not different from median LiTR for grade 2 SP (0%, p=NS). Median LiTR for grade 3 SP was 14.9% and was significantly higher than for grade 2 SP (p<0.001). Similarly, median LiTR for grade 4 SP was 20.7% and was significantly higher than for grade 3 SP (p<0.05). When SP was grade 1 or 2, liver was intra-abdominal in 21 (84%) out of 25 fetuses while it was always intrathoracic for SP 3 or 4., Conclusion: In L-CDH, SP as described represents a simple indirect measurement of intrathoracic position and quantification of liver. more...
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- 2015
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88. Peroxisome proliferator-activated receptor γ-mediated induction of microRNA-145 opposes tumor phenotype in colorectal cancer.
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Panza A, Votino C, Gentile A, Valvano MR, Colangelo T, Pancione M, Micale L, Merla G, Andriulli A, Sabatino L, Vinciguerra M, Prattichizzo C, Mazzoccoli G, Colantuoni V, and Piepoli A
- Subjects
- Aged, Blotting, Western, Cell Cycle, Cell Movement, Cell Proliferation, Cohort Studies, Colon metabolism, Colon pathology, Colorectal Neoplasms metabolism, Colorectal Neoplasms pathology, DNA Primers chemistry, DNA Primers genetics, Female, Humans, Luciferases metabolism, Male, Mutagenesis, PPAR gamma genetics, Phenotype, Promoter Regions, Genetic genetics, RNA, Messenger genetics, Real-Time Polymerase Chain Reaction, Rectum metabolism, Rectum pathology, Reverse Transcriptase Polymerase Chain Reaction, SOX9 Transcription Factor genetics, Transcriptional Activation, Tumor Cells, Cultured, Colorectal Neoplasms genetics, Gene Expression Regulation, Neoplastic, MicroRNAs genetics, PPAR gamma metabolism, SOX9 Transcription Factor metabolism
- Abstract
Unlabelled: MicroRNAs (miRNAs) regulate diverse biological processes by inhibiting translation or inducing degradation of target mRNAs. miR-145 is a candidate tumor suppressor in colorectal carcinoma (CRC). Colorectal carcinogenesis involves deregulation of cellular processes controlled by a number of intertwined chief transcription factors, such as PPARγ and SOX9. Since PPAR family members are able to modulate complex miRNAs networks, we hypothesized a role of miRNA-145 in the interaction between PPARγ and SOX9 in colorectal carcinogenesis. To address this issue, we evaluated gene expression in tissue specimens of CRC patients and we took advantage of invitro models represented by CRC derived cell lines (CaCo2, SW480, HCT116, and HT-29), employing PPARγ activation and/or miRNA-145 ectopic overexpression to analyze how their interplay impact the expression of SOX9 and the development of a malignant phenotype., Results: PPARγ regulates the expression of miR-145 by directly binding to a PPAR response element (PPRE) in its promoter at -1207/-1194bp from the transcription start site. The binding is essential for miR-145 upregulation by PPARγ upon rosiglitazone treatment. Ectopic expression of miR-145, in turn, regulates SOX9 expression through the binding to specific seed motifs. The PPARγ-miR-145-SOX9 axis overarches cell cycle progression, invasiveness and differentiation of CRC derived cell lines. Together, these results suggest that miR-145 is a novel target of PPARγ, acts as a tumor suppressor in CRC cell lines and is a key regulator of intestinal cell differentiation by directly targeting SOX9, a marker of undifferentiated progenitors in the colonic crypts., (Copyright © 2014 Elsevier B.V. All rights reserved.) more...
- Published
- 2014
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89. MicroRNA-130b promotes tumor development and is associated with poor prognosis in colorectal cancer.
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Colangelo T, Fucci A, Votino C, Sabatino L, Pancione M, Laudanna C, Binaschi M, Bigioni M, Maggi CA, Parente D, Forte N, and Colantuoni V
- Subjects
- Animals, Biomarkers, Tumor genetics, Cadherins metabolism, Cell Line, Tumor, Cell Proliferation, Colorectal Neoplasms pathology, Down-Regulation, Epithelial-Mesenchymal Transition genetics, Female, HCT116 Cells, Humans, Mice, Mice, Nude, Neoplasm Invasiveness genetics, Neoplasm Transplantation, Neovascularization, Pathologic genetics, PTEN Phosphohydrolase metabolism, Prognosis, Snail Family Transcription Factors, Transcription Factors metabolism, Up-Regulation, Vascular Endothelial Growth Factor A metabolism, Xenograft Model Antitumor Assays, Cell Transformation, Neoplastic genetics, Colorectal Neoplasms genetics, MicroRNAs genetics, MicroRNAs metabolism, PPAR gamma metabolism
- Abstract
MicroRNA-130b (miR-130b) is involved in several biologic processes; its role in colorectal tumorigenesis has not been addressed so far. Herein, we demonstrate that miR-130b up-regulation exhibits clinical relevance as it is linked to advanced colorectal cancers (CRCs), poor patients' prognosis, and molecular features of enhanced epithelial-mesenchymal transition (EMT) and angiogenesis. miR-130b high-expressing cells develop large, dedifferentiated, and vascularized tumors in mouse xenografts, features that are reverted by intratumor injection of a specific antisense RNA. In contrast, injection of the corresponding mimic in mouse xenografts from miR-130b low-expressing cells increases tumor growth and angiogenic potential while reduces the epithelial hallmarks. These biologic effects are reproduced in human CRC cell lines. We identify peroxisome proliferator-activated receptor γ (PPARγ) as an miR-130b direct target in CRC in vitro and in vivo. Notably, the effects of PPARγ gain- and loss-of-function phenocopy those due to miR-130b down-regulation or up-regulation, respectively, underscoring their biologic relevance. Furthermore, we provide mechanistic evidences that most of the miR-130b-dependent effects are due to PPARγ suppression that in turn deregulates PTEN, E-cadherin, Snail, and vascular endothelial growth factor, key mediators of cell proliferation, EMT, and angiogenesis. Since higher levels of miR-130b are found in advanced tumor stages (III-IV), we propose a novel role of the miR-130b-PPARγ axis in fostering the progression toward more invasive CRCs. Detection of onco-miR-130b and its association with PPARγ may be useful as a prognostic biomarker. Its targeting in vivo should be evaluated as a novel effective therapeutic tool against CRC. more...
- Published
- 2013
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90. Ensemble of gene signatures identifies novel biomarkers in colorectal cancer activated through PPARγ and TNFα signaling.
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Pagnotta SM, Laudanna C, Pancione M, Sabatino L, Votino C, Remo A, Cerulo L, Zoppoli P, Manfrin E, Colantuoni V, and Ceccarelli M
- Subjects
- 5'-Nucleotidase metabolism, Aged, Algorithms, Cell Adhesion genetics, Cell Line, Tumor, Colorectal Neoplasms diagnosis, Colorectal Neoplasms therapy, Computational Biology, Extracellular Matrix metabolism, GPI-Linked Proteins metabolism, Humans, Membrane Proteins metabolism, Oligonucleotide Array Sequence Analysis, Prognosis, Reproducibility of Results, Biomarkers, Tumor genetics, Colorectal Neoplasms genetics, Colorectal Neoplasms pathology, PPAR gamma metabolism, Signal Transduction genetics, Transcriptome, Tumor Necrosis Factor-alpha metabolism
- Abstract
We describe a novel bioinformatic and translational pathology approach, gene Signature Finder Algorithm (gSFA) to identify biomarkers associated with Colorectal Cancer (CRC) survival. Here a robust set of CRC markers is selected by an ensemble method. By using a dataset of 232 gene expression profiles, gSFA discovers 16 highly significant small gene signatures. Analysis of dichotomies generated by the signatures results in a set of 133 samples stably classified in good prognosis group and 56 samples in poor prognosis group, whereas 43 remain unreliably classified. AKAP12, DCBLD2, NT5E and SPON1 are particularly represented in the signatures and selected for validation in vivo on two independent patients cohorts comprising 140 tumor tissues and 60 matched normal tissues. Their expression and regulatory programs are investigated in vitro. We show that the coupled expression of NT5E and DCBLD2 robustly stratifies our patients in two groups (one of which with 100% survival at five years). We show that NT5E is a target of the TNF-α signaling in vitro; the tumor suppressor PPARγ acts as a novel NT5E antagonist that positively and concomitantly regulates DCBLD2 in a cancer cell context-dependent manner. more...
- Published
- 2013
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91. Post-mortem high-field magnetic resonance imaging: effect or various factors.
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Verhoye M, Votino C, Cannie MM, Segers V, Mabiglia C, Cos T, Lipombi D, and Jani JC
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- Female, Fetus pathology, Fetus radiation effects, Freezing adverse effects, Gestational Age, Humans, Image Enhancement, Magnetic Fields adverse effects, Pregnancy, Sensitivity and Specificity, Autopsy methods, Fetal Death diagnosis, Magnetic Resonance Imaging methods
- Abstract
Purpose: To evaluate image quality and diagnostic accuracy of high-field post-mortem (PM) magnetic resonance imaging (MRI) on fetuses below 20 weeks of gestation before and after the freeze-thaw process., Materials and Methods: Nine fetuses were scanned with three different scanning procedures: "fresh", just after termination of pregnancy (TOP), "non-fresh short scan" and "non-fresh long scan" after being kept at -20 °C, followed by a conventional autopsy. The brain, thorax except the heart, heart and abdomen were studied. The qualities of the images for the four different fetal regions and for the three different scanning procedures were reported. Regression analysis was used to investigate the effect on image quality of different factors. Additionally, the diagnostic accuracy was also evaluated., Results: Fetuses at 12.0-19.6 weeks were included. Regression analysis showed that better image quality was correlated to advanced gestation at TOP and scan on fresh fetuses. PM-MRI on fresh fetuses was always diagnostic for the brain and in more than half of cases on non-fresh fetuses and was nearly equally diagnostic for thoracic and abdominal structures., Conclusion: High-field PM-MRI seems to offer a quite reliable alternative to the parents declining conventional PM for fetuses before 20 weeks whether these fetuses are freshly scanned or after being frozen. more...
- Published
- 2013
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92. Right-sided rhabdoid colorectal tumors might be related to the serrated pathway.
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Pancione M, Remo A, Sabatino L, Zanella C, Votino C, Fucci A, Di Blasi A, Lepore G, Daniele B, Fenizia F, Molinari E, Normanno N, Manfrin E, Vendraminelli R, and Colantuoni V
- Subjects
- Adenoma chemistry, Adenoma genetics, Adenoma pathology, Aged, Biomarkers, Tumor analysis, Chromosomes, Human, Pair 22, Colorectal Neoplasms chemistry, Colorectal Neoplasms pathology, Colorectal Neoplasms therapy, CpG Islands, DNA Methylation, DNA Mutational Analysis, Disease Progression, Fatal Outcome, Female, Gene Rearrangement, Genetic Predisposition to Disease, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Intestinal Mucosa chemistry, Intestinal Mucosa pathology, Microsatellite Instability, Mutation, Paraffin Embedding, Phenotype, Polymerase Chain Reaction, Proto-Oncogene Proteins B-raf genetics, Rhabdoid Tumor chemistry, Rhabdoid Tumor pathology, Rhabdoid Tumor therapy, Time Factors, Treatment Outcome, Biomarkers, Tumor genetics, Colorectal Neoplasms genetics, Epigenesis, Genetic, Rhabdoid Tumor genetics, Signal Transduction genetics
- Abstract
Background: Rhabdoid colorectal tumor (RCT) is a rare, highly aggressive neoplasm recurrent in elderly patients, commonly at the caecum. The molecular mechanisms underlying RCT pathogenesis remain poorly elucidated. The differential diagnosis is with the malignant rhabdoid tumors of infancy characterized by genetic inactivation of SMARCB1 (INI1) or deletions of chromosome 22q12 locus., Materials and Methods: To shed light on RCT pathogenesis, we investigated genetic and epigenetic alterations in two cases of pure and composite RCT and compared them with the profiles of matched adenomas and normal mucosa. Immunohistochemical analysis, FISH, methylation specific PCR and DNA sequencing analysis were performed on paraffin-embedded tissues., Results: Loss of epithelial markers, (CK20, CDX2 and E-cadherin) and intense vimentin expression was observed in RCTs but neither in the normal mucosa or adenomas. INI1 expression was detected in normal mucosa, adenomas and retained in pure RCT, while it was undetected in composite RCT. Rearrangement of the 22q12 locus was found only in pure RCT. The APC/β-catenin pathway was not altered, while MLH1 immunostaining was negative in RCTs and positive in adenomas and normal mucosa. These expression profiles were associated with V600E BRAF mutation, a progressive accumulation of promoter methylation at specific CIMP loci and additional genes from the normal mucosa to tubular adenoma and RCT., Conclusions: Right-sided RCT could be characterized by epigenetic events and molecular features likely similar to those occurring in the serrated pathway and associated with epithelial-mesenchymal transition. These extremely rare tumors may benefit from the use of new biological molecules specific for colorectal carcinoma., Virtual Slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1641385210804556. more...
- Published
- 2013
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93. Signaling molecules in the fetal rabbit model for congenital diaphragmatic hernia.
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Vuckovic A, Roubliova XI, Votino C, Naeije R, and Jani JC
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- Animals, Cyclic Nucleotide Phosphodiesterases, Type 5 biosynthesis, Cyclic Nucleotide Phosphodiesterases, Type 5 genetics, Disease Models, Animal, Female, Fetal Organ Maturity physiology, Fetus metabolism, Gene Expression Profiling, Hernia, Diaphragmatic metabolism, Hernia, Diaphragmatic surgery, Humans, Nitric Oxide Synthase Type III biosynthesis, Nitric Oxide Synthase Type III genetics, Pregnancy, Protein-Lysine 6-Oxidase biosynthesis, Protein-Lysine 6-Oxidase genetics, Rabbits, Tropoelastin biosynthesis, Tropoelastin genetics, Gene Expression Regulation, Developmental physiology, Hernias, Diaphragmatic, Congenital, Lung growth & development, Lung metabolism, Signal Transduction physiology
- Abstract
Rationale and Objectives: Little is known about molecular changes in lungs of fetal rabbits with surgically induced diaphragmatic hernia (DH). Therefore, we examined in this model gene expressions of pivotal molecules for the developing lung., Methods: At day 23 of gestation, DH was created in 12 fetuses from 4 does. Both lungs from six live DH fetuses and from six unoperated controls were harvested and weighed at term. Transcription of 15 genes involved in alveolarization, angiogenesis, regulation of vascular tone, or epithelial maturation was investigated by real-time quantitative polymerase chain reaction., Main Results: DH decreased lung-to-body weight ratio (P < 0.001). A bilateral downregulation was seen for genes encoding for tropoelastin (P < 0.01), lysyl oxidase (P < 0.05), fibulin 5 (P < 0.05), and cGMP specific phosphodiesterase 5 (P < 0.05). Lower mRNA levels for endothelial nitric oxide synthase occurred in the ipsilateral lung (P < 0.05)., Conclusions: Experimental DH in fetal rabbits disrupted transcription of genes implicated in lung growth and function. Similarities with the human disease make this model appropriate for investigation of new prenatal therapies., (Copyright © 2012 Wiley Periodicals, Inc.) more...
- Published
- 2012
- Full Text
- View/download PDF
94. The role of peroxisome proliferator-activated receptors in the esophageal, gastric, and colorectal cancer.
- Author
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Fucci A, Colangelo T, Votino C, Pancione M, Sabatino L, and Colantuoni V
- Abstract
Tumors of the gastrointestinal tract are among the most frequent human malignancies and account for approximately 30% of cancer-related deaths worldwide. Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that control diverse cellular functions such as proliferation, differentiation, and cell death. Owing to their involvement in so many processes, they play crucial roles also in the development and physiology of the gastrointestinal tract. Consistently, PPARs deregulation has been implicated in several pathophysiological conditions, including chronic inflammation and cancer development. This paper summarizes the current knowledge on the role that the various PPAR isoforms play in the pathogenesis of the esophageal, gastric, and intestinal cancer. Elucidation of the molecular mechanisms underlying PPARs' signaling pathways will provide insights into their possible use as predictive biomarkers in the initial stages of the process. In addition, this understanding will provide the basis for new molecular targets in cancer therapy and chemoprevention. more...
- Published
- 2012
- Full Text
- View/download PDF
95. Successful treatment of a severe second trimester fetomaternal hemorrhage by repeated fetal intravascular transfusions.
- Author
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Votino C, Mirlesse V, Gourand L, Parnet-Mathieu F, Bessières B, and Daffos F
- Subjects
- Adult, Anemia therapy, Blood Flow Velocity, Female, Hematocrit, Humans, Infant, Newborn, Middle Cerebral Artery physiology, Pregnancy, Pregnancy Trimester, Second, Severity of Illness Index, Blood Transfusion, Intrauterine, Fetomaternal Transfusion therapy
- Abstract
Objective: It was the aim of this study to report a case of fetomaternal hemorrhage (FMH) that was successfully treated with fetal intravascular transfusions in which the middle cerebral artery peak systolic velocity (MCA-PSV) detected fetal anemia., Methods: A massive FMH occurred twice in a healthy 33-year-old pregnant woman at 26 and 29 weeks of gestation with no evident cause. Four repeated intravascular transfusions were performed. The MCA-PSV increased in the presence of anemia and decreased following correction of fetal hematocrit., Results: A healthy neonate was delivered at 33 weeks of gestation., Conclusion: MCA-PSV detected fetal anemia both before the first transfusion and following the next intravascular transfusions. In our case, the change in fetal blood viscosity following transfusion with adult blood did not affect the MCA-PSV value for detection of fetal anemia., (Copyright 2009 S. Karger AG, Basel.) more...
- Published
- 2008
- Full Text
- View/download PDF
96. Is uterine artery embolization for cervical ectopic pregnancy always safe?
- Author
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Martinelli P, Maruotti GM, Oppedisano R, Agangi A, Mazzarelli LL, Votino C, Quarantelli M, and Iaccarino V
- Subjects
- Adult, Arteries surgery, Cervix Uteri pathology, Dilatation and Curettage, Electrocoagulation adverse effects, Electrocoagulation methods, Female, Humans, Hysterectomy, Pregnancy, Cervix Uteri blood supply, Embolization, Therapeutic, Pregnancy, Ectopic therapy, Uterus blood supply
- Abstract
The study objective was to assess the feasibility and the efficacy of bilateral uterine artery embolization (BUAE) for the treatment of cervical pregnancy. The design was a series of 3 cases of viable cervical pregnancy diagnosed by transvaginal ultrasonography and treated by means of BUAE and subsequent uterine curettage. Three women with viable cervical pregnancy underwent BUAE and subsequent uterine curettage in the department of obstetrics and gynecology, High Risk Pregnancy Center, University "Federico II" of Naples. Measurements included surgical outcomes and preservation of fertility. The treatment was effective in all cases. Two patients resumed normal menstruation about 1 month after the procedure, whereas 1 patient underwent a hysterectomy 2 weeks after embolization because of acute ischemic degeneration of a concomitant myoma. The conservative management of cervical pregnancy with angiographic BUAE is a feasible and effective option, even if subsequent hysterectomy may be required. Counseling is necessary. more...
- Published
- 2007
- Full Text
- View/download PDF
97. [Effect of estriol treatment per vaginam before Burch culposuspension].
- Author
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Palomba S, Napolitano V, Sammartino A, Di Spiezio Sardo A, Vassallo M, Mandato V, Ruoto M, Acunzo G, Votino C, and Nappi C
- Subjects
- Administration, Intravaginal, Female, Follow-Up Studies, Humans, Middle Aged, Postmenopause, Prospective Studies, Time Factors, Ultrasonography, Urinary Incontinence, Stress diagnostic imaging, Urinary Incontinence, Stress physiopathology, Urodynamics, Estriol administration & dosage, Urinary Incontinence, Stress surgery, Vagina surgery
- Abstract
Background: To assess the effect of estriol treatment per vaginam before Burch culposuspension in postmenopausal women with stress urinary incontinence (IUS)., Design: prospective randomised study., Setting: Department of Gynaecology, Obstetrics and Physiopathology of Human Reproduction-Medical School-University of Naples Federico II ., Patients: twenty women in postmenopause at least from five years with a urogenital symptomatology due to IUS., Interventions: women were randomised into one of two groups (treated or control) and they were submitted to an evaluation of vulva and vagina trophism. All the women were submitted to a urodynamic examination and to a transvaginal ultrasonography with evaluation of pubis-bladder neck distance, bladder and proximal urethra position, before treatment, one week before the operation and after six months from the same operation., Evaluations: subjective symptomatology and urodynamic parameters between treated and control groups before and after operation., Results: After 12 weeks of treatment, a significant improvement of subjective symptomatology and a not significant improvement of all the urodynamic parameters in the treated group in comparison with the control group have been demonstrated, while any anatomic alteration compared with the basal hasn t been observed. After six months from the operation in all the women a significant reduction of subjective quantity of urine lost after a strain has been demonstrated and significant variations of urodynamic parameters without significant differences between treated group and control group were also observed., Conclusions: The estriol treatment per vaginam is not so effective on the result of Burch culposuspension in postmenopausal women with IUS. more...
- Published
- 2001
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