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A dysfunctional gut microbiota-brain axis is emerging as a potential pathogenic mechanism in epilepsy, particularly in pediatric forms of epilepsy. To add new insights into gut-related changes in acquired epilepsy that develops early in life, we used a multi-omics approach in a rat model with a 56% incidence of epilepsy.The presence of spontaneous seizures was assessed in adult rats (n = 46) 5 months after status epilepticus induced by intra-amygdala kainate at postnatal day 13, by 2 weeks (24/7) ECoG monitoring. Twenty-six rats developed epilepsy (Epi) while the remaining 20 rats (No-Epi) did not show spontaneous seizures. At the end of ECoG monitoring, all rats and their sham controls (n = 20) were sacrificed for quantitative histopathological and immunohistochemical analyses of the gut structure, glia and macrophages, as well as RTqPCR analysis of inflammation/oxidative stress markers.By comparing Epi, No-Epi rats, and sham controls, we found structural, cellular, and molecular alterations reflecting a dysfunctional gut, which were specifically associated with epilepsy. In particular, the villus height-to-crypt depth ratio and number of Goblet cells were reduced in the duodenum of Epi rats vs both No-Epi rats and sham controls (p

IntroductionShigella is the etiologic agent of a bacillary dysentery known as shigellosis, which causes millions of infections and thousands of deaths worldwide each year due to Shigella’s unique lifestyle within intestinal epithelial cells. Cell adhesion/invasion assays have been extensively used not only to identify targets mediating host-pathogen interaction, but also to evaluate the ability of Shigella-specific antibodies to reduce virulence. However, these assays are time-consuming and labor-intensive and fail to assess differences at the single-cell level.Objectives and methodsHere, we developed a simple, fast and high-content method named visual Adhesion/Invasion Inhibition Assay (vAIA) to measure the ability of anti-Shigellaantibodies to inhibit bacterial adhesion to and invasion of epithelial cells by using the confocal microscope Opera Phenix.ResultsWe showed that vAIA performed well with a pooled human serum from subjects challenged with S. sonnei and that a specific anti-IpaD monoclonal antibody effectively reduced bacterial virulence in a dose-dependent manner.DiscussionvAIA can therefore inform on the functionality of polyclonal and monoclonal responses thereby supporting the discovery of pathogenicity mechanisms and the development of candidate vaccines and immunotherapies. Lastly, this assay is very versatile and may be easily applied to other Shigella species or serotypes and to different pathogens.

Abstract Background The recent pandemic outbursts, due to SARS-CoV-2, have highlighted once more the central role of the inflammatory process in the propagation of viral infection. The main consequence of COVID-19 is the induction of a diffuse pro-inflammatory state, also defined as a cytokine storm, which affects different organs, but mostly the lungs. We aimed to prove the efficacy of cinnamaldehyde, the active compound of cinnamon, as an anti-inflammatory compound, able to reduce SARS-CoV-2 induced cytokine storm. Results We enrolled 53 COVID-19 patients hospitalized for respiratory failure. The cohort was composed by 39 males and 13 females, aged 65.0 ± 9.8 years. We reported that COVID-19 patients have significantly higher IL-1β and IL-6 plasma levels compared to non-COVID-19 pneumonia patients. In addition, human mononuclear cells (PBMCs) isolated from SARS-CoV-2 infected patients are significantly more prone to release pro-inflammatory cytokines upon stimuli. We demonstrated, using in vitro cell models, that macrophages are responsible for mediating the pro-inflammatory cytokine storm while lung cells support SARS-CoV-2 replication upon viral infection. In this context, cinnamaldehyde administration significantly reduces SARS-CoV-2-related inflammation by inhibiting NLRP3 mediated IL-1β release in both PBMCs and THP-1 macrophages, as well as viral replication in CaLu-3 epithelial cells. Lastly, aerosol-administered cinnamaldehyde was able to significantly reduce IL-1β release in an in vivo lung-inflammatory model. Conclusion The obtained results suggest the possible use of cinnamaldehyde as a co-adjuvant preventive treatment for COVID-19 disease together with vaccination, but also as a promising dietary supplement to reduce, more broadly, viral induced inflammation.

Epilepsy is a common and serious neurological disorder, with many different constituent conditions characterized by their electro clinical, imaging, and genetic features. MRI has been fundamental in advancing our understanding of brain processes in the epilepsies. Smaller-scale studies have identified many interesting imaging phenomena, with implications both for understanding pathophysiology and improving clinical care. Through the infrastructure and concepts now well-established by the ENIGMA Consortium, ENIGMA-Epilepsy was established to strengthen epilepsy neuroscience by greatly increasing sample sizes, leveraging ideas and methods established in other ENIGMA projects, and generating a body of collaborating scientists and clinicians to drive forward robust research. Here we review published, current, and future projects, that include structural MRI, diffusion tensor imaging (DTI), and resting state functional MRI (rsfMRI), and that employ advanced methods including structural covariance, and event-based modeling analysis. We explore age of onset- and duration-related features, as well as phenomena-specific work focusing on particular epilepsy syndromes or phenotypes, multimodal analyses focused on understanding the biology of disease progression, and deep learning approaches. We encourage groups who may be interested in participating to make contact to further grow and develop ENIGMA-Epilepsy.

This Special Issue is dedicated to the 2nd International Conference on “Multilingual digital terminology today: Design, representation formats, and management systems” (MDTT 2023) (Di Nunzio et al [...]

Human behavior strongly influences the spread of infectious diseases: understanding the interplay between epidemic dynamics and adaptive behaviors is essential to improve response strategies to epidemics, with the goal of containing the epidemic while preserving a sufficient level of operativeness in the population. Through activity-driven temporal networks, we formulate a general framework which models a wide range of adaptive behaviors and mitigation strategies, observed in real populations. We analytically derive the conditions for a widespread diffusion of epidemics in the presence of arbitrary adaptive behaviors, highlighting the crucial role of correlations between agents behavior in the infected and in the susceptible state. We focus on the effects of sick leave, comparing the effectiveness of different strategies in reducing the impact of the epidemic and preserving the system operativeness. We show the critical relevance of heterogeneity in individual behavior: in homogeneous networks, all sick-leave strategies are equivalent and poorly effective, while in heterogeneous networks, strategies targeting the most vulnerable nodes are able to effectively mitigate the epidemic, also avoiding a deterioration in system activity and maintaining a low level of absenteeism. Interestingly, with targeted strategies both the minimum of population activity and the maximum of absenteeism anticipate the infection peak, which is effectively flattened and delayed, so that full operativeness is almost restored when the infection peak arrives. We also provide realistic estimates of the model parameters for influenza-like illness, thereby suggesting strategies for managing epidemics and absenteeism in realistic populations.

A person-oriented approach was applied to the study of early adolescents' learning orientations to identify different profiles and their association with school achievement. A total of 244 middle-school students (male: 128; female: 116; M-age ± SD = 12.51 ± 0.93) completed a self-report questionnaire to explore cognitive, metacognitive, and affective dimensions of learning orientations. School achievements were collected in a range of school subjects. Cluster analyses and one-way MANOVA were carried out to verify the existence of profiles. Chi-square tests were used to test the association between profiles of learning orientations and school achievement. The results showed two profiles differently associated with school achievement: students "Dragged by the current" (Profile 1) showed low school achievement across school subjects, meanwhile students "At the helm" (Profile 2) reached excellent school achievement. In promoting middle-school students' achievement, it is important for teachers to consider students' profiles of learning orientations.

Working in pairs can create favourable conditions for students’ involvement in reading comprehension strategies. The literature has shown practical advantages of organisation in pairs in classrooms, assimilating the functioning of the pair to that of the "good reader"(Hall et al., 1989). Following previous research inspired by a Reciprocal Teaching protocol (Calvani & Chiappetta Cajola, 2019), the goal of the present work was to analyse the interactions of some pairs. The processes of understanding the text have been described when the pairs worked in two different procedures: when both students play an alternating tutorial role (tutorial procedure) and when two students tackle the comprehension tasks jointly (joint procedure). The interactions between the two students in the pairs (cognitive, regulative, and affective) are significantly focused on the texts, both in low-skilled pairs and in those with more advanced skills, with a greater prevalence in the joint procedure compared to the tutorial one.

Infantile spasms (IS) is a devastating epilepsy syndrome that typically begins in the first year of life. Symptoms consist of stereotypical spasms, developmental delay, and electroencephalogram (EEG) that may demonstrate Hypsarhythmia. Current therapeutic approaches are not always effective, and there is no reliable way to predict which patient will respond to therapy. Given this disorder's complexity and the potential impact of a disease-modifying approach, Citizens United for Research in Epilepsy (CURE) employed a "team science" approach to advance the understanding of IS pathology and explore therapeutic modalities that might lead to the development of new ways to potentially prevent spasms and Hypsarhythmia. This approach was a first-of-its-kind collaborative initiative in epilepsy. The IS initiative funded 8 investigative teams over the course of 1-3 years. Projects included the following: discovery on the basic biology of IS, discovery of novel therapeutic targets, cross-validation of targets, discovery of biomarkers, and prognosis and treatment of IS. The combined efforts of a strong investigative team led to numerous advances in understanding the neural pathways underlying IS, testing of small molecules in preclinical models of IS and generated preliminary data on potential biomarkers. Thus far, the initiative has resulted in over 19 publications and subsequent funding for several investigators. Investigators reported that the IS initiative generally affected their research positively due to its collaborative and iterative nature. It also provided a unique opportunity to mentor junior investigators with an interest in translational research. Learnings included the need for a dedicated project manager and more transparent and real-time communication with investigators. The CURE IS initiative represents a unique approach to fund scientific discoveries on epilepsy. It brought together an interdisciplinary group of investigators-who otherwise would not have collaborated-to find transformative therapies for IS. Learnings from this initiative are being utilized for subsequent initiatives at CURE.

Climate change is with us. As professionals who place value on evidence-based practice, climate change is something we cannot ignore. The current pandemic of the novel coronavirus, SARS-CoV-2, has demonstrated how global crises can arise suddenly and have a significant impact on public health. Global warming, a chronic process punctuated by acute episodes of extreme weather events, is an insidious global health crisis needing at least as much attention. Many neurological diseases are complex chronic conditions influenced at many levels by changes in the environment. This review aimed to collate and evaluate reports from clinical and basic science about the relationship between climate change and epilepsy. The keywords climate change, seasonal variation, temperature, humidity, thermoregulation, biorhythm, gene, circadian rhythm, heat, and weather were used to search the published evidence. A number of climatic variables are associated with increased seizure frequency in people with epilepsy. Climate change-induced increase in seizure precipitants such as fevers, stress, and sleep deprivation (e.g. as a result of more frequent extreme weather events) or vector-borne infections may trigger or exacerbate seizures, lead to deterioration of seizure control, and affect neurological, cerebrovascular, or cardiovascular comorbidities and risk of sudden unexpected death in epilepsy. Risks are likely to be modified by many factors, ranging from individual genetic variation and temperature-dependent channel function, to housing quality and global supply chains. According to the results of the limited number of experimental studies with animal models of seizures or epilepsy, different seizure types appear to have distinct susceptibility to seasonal influences. Increased body temperature, whether in the context of fever or not, has a critical role in seizure threshold and seizure-related brain damage. Links between climate change and epilepsy are likely to be multifactorial, complex, and often indirect, which makes predictions difficult. We need more data on possible climate-driven altered risks for seizures, epilepsy, and epileptogenesis, to identify underlying mechanisms at systems, cellular, and molecular levels for better understanding of the impact of climate change on epilepsy. Further focussed data would help us to develop evidence for mitigation methods to do more to protect people with epilepsy from the effects of climate change.

Abstract Background Globally, it has been reported that different social determinants of health -structural, sociodemographic, economic, living conditions and cultural factors- may affect opportunities to adhere to prevention measures against SARS-CoV-2. The objective of this study was to explore the perceptions around barriers and facilitators for adherence to COVID-19 prevention measures among the adult population residing in three large cities in Chile from a social determinants of health perspective. Methods Qualitative paradigm, multiple case-study design. Online semi-structured interviews were conducted with men and women aged 18 and over from different socioeconomic groups residing in three large cities. For participant recruitment and selection, purposive contacts were made based on community and social media networks, followed by snowball sampling. Saturation was reached at 61 participants, after which a thematic analysis was carried out with the support of AtlasTi software. The Ethics Committee of the Universidad del Desarrollo in Chile approved this study. Results The main perceived barriers to adherence to COVID-19 preventive measures are linked to structural social determinants of health such as income, occupation, gender, access to basic supplies, and housing. Perceived facilitators are the fear of contagion and the incorporation of measures into daily habits. The social communication of preventive measures by health authorities is perceived as punitive, affecting adherence once the fear of contagion decreased in the country. It is also perceived that the recommended preventive measures are disconnected from communities’ cultural practices and people´s identity, as well as affected by gender inequities and socioeconomic conditions that stakeholders in the country do not sufficiently address. Conclusion Study findings suggest that adherence to preventive measures, such as social distancing, mask use, and hand washing, could be promoted through their incorporation into the daily life habits of people and communities. These measures should consider the structural social determinants that generate multiple barriers to adherence, like poverty, occupational risks, and overcrowding. Socio-cultural dimensions of health and everyday risks need further understanding among the different communities in the country, allowing for differences in viewpoints and practices based on gender, age, place, and social identity.

Semic analysis is a linguistic technique aimed at methodically factorizing the meaning of terms into a collection of minimum non-decomposable atoms of meaning. In this study, we propose a methodology targeted at enhancing the systematicity of semic analysis of medical terminology in order to increase the quality of the creation of the set of atoms of meaning and improve the identification of concepts, as well as enhance specialized domain studies. Our approach is based on: (1) a semi-automatic domain-specific corpus-based extraction of semes, (2) the application of the property of termhood to address the diaphasic and the diastratic variations of language, (3) the automatic lemmatization of semes, and (4) seme weighting to establish the order of semes in the sememe. The paper explores the distinction between denotative and connotative semes, offering insights into polysemy and polyreferentiality in medical terminology.

Students' conceptions of learning represent an influential factor for learning, yet the few existing studies used measures with limited validity and lacked to provide a model for middle school students. This research aimed to provide a preliminary validation of 'Learning Conception Questionnaire' (LCQ) by Liverta Sempio and Marchetti (2001) aimed to measure conceptions of learning in a holistic way by including belief, academic emotion, and causal attributions. In the current study, the factor structure was tested in a sample of 212 middle school students. Exploratory factor analysis (EFAs) and Confirmatory factor analysis (CFAs) showed that the factor structure of the comprehensive measure of conceptions of learning used could be described across three domains (Belief: Comparative Fit Index [CFI] = 0.98, Standardised Root Mean Square Residual [SRMR] = 0.06; Emotions: CFI = 0.89, SRMR = 0.07; Causal attribution: CFI = 0.92, SRMR = 0.06), with significant relationships. Implications and future ways of research were discussed.

ABSTRACT The traditional paradigm of education, although outdated, still supports Soil Educational procedures in several Brazilian universities. However, the strategic documents of these institutions (Institutional Development Plan-IDP, Institutional Political Project-IPP and Pedagogical Projects of Courses-PPC's) support a critical vision of education, which converges with the emancipatory paradigm and with the purpose of Soil Education (SE), which is the formation and transformation of subjects for conscious actions in reality. To overcome this problem, we propose educational procedures guided by emancipatory principles for two disciplines related to the subject of soils in Higher Education (HE) in a traditional paradigm structure, with the objective of meeting the conception of education supported in the strategic documents of a Brazilian public university and achieving the purpose of SE. A bibliographical research and documental analysis of the strategic documents were carried out to form a theoretical and methodological base. In addition, emancipatory principles were considered in the elements that made up the educational procedures in SE. The proposal was analyzed and discussed based on the behavior of these principles in the structure of the disciplines. To verify how the proposal articulates with the conception of education defended by the university, the analysis started from the didactic-pedagogical aspects expressed in the IDP, IPP, PPC's and Teaching Plans of the disciplines involved in the research. We emphasize that it was possible to propose and apply educational procedures on soils with emancipatory principles within a traditional paradigm structure. Some principles were considered in essence, while others could not be considered or were only partially considered, showing that the traditional paradigm limits principles that go against its foundations. The proposal responded more to the concept of critical education defended in the strategic documents than the Soil Educational procedures with traditional principles elaborated and used by professors, since the proposal considered the student’s protagonism, the constitution of their autonomy, knowledge as relational, research as an educational principle, interculturality and procedural and continuous evaluation. On the other hand, procedures with traditional principles considered students as passive subjects, knowledge was seen as fragmented and their approach was made in an authoritarian way, disregarding students’ knowledge and the evaluation was quantitative. Furthermore, the convergence of educational intentionality between Education in Soils and education with emancipatory principles allows us to state that the emancipatory paradigm was an alternative to achieve the educational and formative purpose in educational processes on soils.

The latency between traumatic brain injury (TBI) and the onset of epilepsy (PTE) represents an opportunity for counteracting epileptogenesis. Antiepileptogenesis trials are hampered by the lack of sensitive biomarkers that allow to enrich patient's population at-risk for PTE. We aimed to assess whether specific ECoG signals predict PTE in a clinically relevant mouse model with ∼60% epilepsy incidence. TBI was provoked in adult CD1 male mice by controlled cortical impact on the left parieto-temporal cortex, then mice were implanted with two perilesional cortical screw electrodes and two similar electrodes in the hemisphere contralateral to the lesion site. Acute seizures and spikes/sharp waves were ECoG-recorded during 1 week post-TBI. These early ECoG events were analyzed according to PTE incidence as assessed by measuring spontaneous recurrent seizures (SRS) at 5 months post-TBI. We found that incidence, number and duration of acute seizures during 3 days post-TBI were similar in PTE mice and mice not developing epilepsy (No SRS mice). Control mice with cortical electrodes (naïve, n = 5) or with electrodes and craniotomy (sham, n = 5) exhibited acute seizures but did not develop epilepsy. The daily number of spikes/sharp waves at the perilesional electrodes was increased similarly in PTE (n = 15) and No SRS (n = 8) mice vs controls (p