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Your search keyword '"Vegesna, A. K."' showing total 76 results
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76 results on '"Vegesna, A. K."'

67. Use of Intravenous Fluorescein to Localize Gastrointestinal Ischemia

Author

Vegesna, Anil K., primary, Gary, Cohen, additional, Kalra, Amit, additional, Besetty, Ramashesai, additional, Gomez, Javier, additional, Knight, Linda, additional, Iqbal, Zahid, additional, Bright, Lewis, additional, Naseer, Imran, additional, Korrimili, Annapurna, additional, Haroon, Muhammad Y., additional, Farooq, Umar, additional, and Miller, Larry S., additional

Published
2007
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68. pH-activatable near-infra red fluorescent probes for detection of lysosomal pH inside living cells.

Author

Vegesna, Giri K., Janjanam, Jagadeesh, Jianheng Bi, Fen-Tair Luo, Jingtuo Zhang, Connor Olds, Tiwari, Ashutosh, and Haiying Liu

Abstract

Four near-infrared fluorescent probes (A, B, C and D) have been synthesized, characterized, and evaluated for detection of lysosomal pH inside living cells. The fluorescent probes display highly sensitive and selective fluorescent response to acidic pH as the acidic pH results in drastic structural changes from spirocyclic (non-fluorescent) forms to ring-opening (fluorescent) forms of the fluorescent probes. The fluorescence intensities of the fluorescent probes (B, C and D) increase significantly by more than 200-fold from pH 7.4 to 4.2. The fluorescent probe D bearing the N-(2-hydroxyethyl) ethylene amide residue possesses the advantages of high sensitivity, excellent photostability, good cell membrane permeability, strong pH dependence, and low auto-fluorescence background. It has been successfully applied to selectively stain lysosomes and detect lysosomal pH changes inside normal endothelial and breast cancer cells. [ABSTRACT FROM AUTHOR]

Published
2014
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70. Comparison of human and porcine gastric clasp and sling fiber contraction by M2 and M3 muscarinic receptors.

Author

Vegesna, Anit K., Braverman, Alan S., Miller, Larry S., Tallarida, Ronald J., Tiwana, Mansoor I., Khayyam, Umar, and Ruggieri, Sr., Michael R.

Subjects
*ESOPHAGOGASTRIC junction, *MUSCARINIC receptors, *SMOOTH muscle, *DISEASE research, *SWINE diseases, *MUSCLE contraction
Abstract

To compare the gastroesophageal junction of the human with the pig, M2 and M3 receptor densities and the potencies of M2 and M3 muscarinic receptor subtype selective antagonists were determined in gastric clasp and sling smooth muscle fibers. Total muscarinic and M2 receptors are higher in pig than human clasp and sling fibers. M3 receptors are higher in human compared with pig sling fibers but lower in human compared with pig clasp fibers. Clasp fibers have fewer M3q receptors than sling fibers in both humans and pigs. Similar to human clasp fibers, pig clasp fibers contract significantly less than pig sling fibers. Analysis of the methoctramine Schild plot suggests that M2 receptors are involved in mediating contraction in pig clasp and sling fibers. Darifenacin potency suggests that M3 receptors mediate contraction in pig sling fibers and that M2 and M3 receptors mediate contraction in pig clasp fibers. Taken together, the data suggest that both M2 and M3 muscarinic receptors mediate the contraction in both pig clasp and sling fibers similar to human clasp and sling fibers. [ABSTRACT FROM AUTHOR]

Published
2010
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72. Quantitation of the contractile response mediated by two receptors: M2 and M3 muscarinic receptor-mediated contractions of human gastroesophageal smooth muscle.

Author

Braverman, Alan S, Miller, Larry S, Vegesna, Anil K, Tiwana, Mansoor I, Tallarida, Ronald J, and Ruggieri, Michael R

Abstract

Although muscarinic receptors are known to mediate tonic contraction of human gastrointestinal tract smooth muscle, the receptor subtypes that mediate the tonic contractions are not entirely clear. Whole human stomachs with attached esophagus were procured from organ transplant donors. Cholinergic contractile responses of clasp, sling, lower esophageal circular (LEC), midesophageal circular (MEC), and midesophageal longitudinal (MEL) muscle strips were determined. Sling fibers contracted greater than the other fibers. Total, M(2) and M(3) muscarinic receptor density was determined for each of these dissections by immunoprecipitation. M(2) receptor density is greatest in the sling fibers, followed by clasp, LEC, MEC, and then MEL, whereas M(3) density is greatest in LEC, followed by MEL, MEC, sling, and then clasp. The potency of subtype-selective antagonists to inhibit bethanechol-induced contraction was calculated by Schild analysis to determine which muscarinic receptor subtypes contribute to contraction. The results suggest both M(2) and M(3) receptors mediate contraction in clasp and sling fibers. Thus, this type of analysis in which multiple receptors mediate the contractile response is inappropriate, and an analysis method relating dual occupation of M(2) and M(3) receptors to contraction is presented. Using this new method of analysis, it was found that the M(2) muscarinic receptor plays a greater role in mediating contraction of clasp and sling fibers than in LEC, MEC, and MEL muscles in which the M(3) receptor predominantly mediates contraction.

Published
2009
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