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323 results on '"Van den Abbeele, Pieter"'

52. Long-Term Lactulose Administration Improves Dysbiosis Induced by Antibiotic and C. difficile in the PathoGut TM SHIME Model.

Author

Calatayud, Marta, Duysburgh, Cindy, Van den Abbeele, Pieter, Franckenstein, Dennis, Kuchina-Koch, Angelika, and Marzorati, Massimo

Subjects
CLOSTRIDIOIDES difficile, LACTULOSE, BACTERIAL colonies, ANTIBIOTICS, DYSBIOSIS
Abstract

Clostridioides difficile infection (CDI) is the leading cause of antibiotic-associated diarrhea and an important nosocomial infection with different severity degrees. Disruption of the gut microbiota by broad-spectrum antibiotics creates a proper environment for C. difficile colonization, proliferation, and clinical disease onset. Restoration of the gut microbial ecosystem through prebiotic interventions can constitute an effective complementary treatment of CDI. Using an adapted simulator of the human gut microbial ecosystem, the PathoGutTM SHIME, the effect of different long-term and repeated dose lactulose treatments was tested on C. difficile germination and growth in antibiotic-induced dysbiotic gut microbiota environments. The results showed that lactulose reduced the growth of viable C. difficile cells following clindamycin treatment, shifted the antibiotic-induced dysbiotic microbial community, and stimulated the production of health-promoting metabolites (especially butyrate). Recovery of the gut microenvironment by long-term lactulose administration following CDI was also linked to lactate production, decrease in pH and modulation of bile salt metabolism. At a structural level, lactulose showed a significant bifidogenic potential and restored key commensal members of the gut ecosystem such as Lactobacillaceae, Veillonellaceae and Lachnospiraceae. These results support further human intervention studies aiming to validate the in vitro beneficial effects of lactulose on gut microbiome recovery during antibiotic exposure and CDI. [ABSTRACT FROM AUTHOR]

Published
2022
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56. A randomized, placebo-controlled trial investigating the acute and chronic benefits of American Ginseng (Cereboost®) on mood and cognition in healthy young adults, including in vitro investigation of gut microbiota changes as a possible mechanism of action

Author

Bell, Lynne, primary, Whyte, Adrian, additional, Duysburgh, Cindy, additional, Marzorati, Massimo, additional, Van den Abbeele, Pieter, additional, Le Cozannet, Romain, additional, Fança-Berthon, Pascale, additional, Fromentin, Emilie, additional, and Williams, Claire, additional

Published
2021
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66. impact of environmental pH on the gut microbiota community structure and short chain fatty acid production.

Author

Firrman, Jenni, Liu, LinShu, Mahalak, Karley, Tanes, Ceylan, Bittinger, Kyle, Tu, Vincent, Bobokalonov, Jamshed, Mattei, Lisa, Zhang, Huanjia, and Van den Abbeele, Pieter

Subjects
SHORT-chain fatty acids, GUT microbiome, BUTYRIC acid, ACETIC acid, VALERIC acid, PROPIONIC acid
Abstract

Environmental pH is a critical parameter for maintenance of the gut microbiota. Here, the impact of pH on the gut microbiota luminal and mucosal community structure and short chain fatty acid (SCFA) production was evaluated i n vitro , and data compiled to reveal a donor-independent response to an increase or decrease in environmental pH. The results found that raising environmental pH significantly increased luminal community richness and decreased mucosal community evenness. This corresponded with an increased abundance of Ruminococcaceae Ruminococcus and Erysipelotrichaceae Erysipelatoclostridium , and a decreased abundance of Coriobacteriaceae Collinsella and Enterobacteriaceae Shigella for both the luminal and mucosal communities. Total SCFA levels were significantly higher, primarily due to an increase in acetic and 2-methylbutanoic acids. Lowering pH decreased luminal community evenness and decreased mucosal community evenness and richness. This corresponded with an increased abundance of Lachnospiraceae Enterocloster, Veillonellaceae Megasphaera, Veillonellaceae Sporomusa, Erysipelotrichaceae Eubacterium , and Alcaligenaceae Sutterella , and decreased abundance of Odoribacteraceae Butyricimonas, Fusobacteriaceae Fusobacterium, Veillonellaceae Phascolarctobacterium , and multiple Enterobacteriaceae species for both the luminal and mucosal communities. Total SCFA levels were significantly lower, with an observed drop in acetic and propionic acids, and increased butyric and valeric acids. Taken together, these results indicate that alterations to environmental pH can modulate the gut microbiota community structure and function, and some changes may occur in a donor-independent manner. [ABSTRACT FROM AUTHOR]

Published
2022
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68. Development of an Affordable, Sustainable and Efficacious Plant-Based Immunomodulatory Food Ingredient Based on Bell Pepper or Carrot RG-I Pectic Polysaccharides

Author

McKay, Sue, primary, Oranje, Paul, additional, Helin, Jari, additional, Koek, Jean H., additional, Kreijveld, Ellen, additional, van den Abbeele, Pieter, additional, Pohl, Ute, additional, Bothe, Gordana, additional, Tzoumaki, Maria, additional, Aparicio-Vergara, Marcela, additional, Mercenier, Annick, additional, Schols, Henk, additional, and Albers, Ruud, additional

Published
2021
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70. In vitro–in vivo Validation of Stimulatory Effect of Oat Ingredients on Lactobacilli

Author

Duysburgh, Cindy, primary, Van den Abbeele, Pieter, additional, Kamil, Alison, additional, Fleige, Lisa, additional, De Chavez, Peter John, additional, Chu, YiFang, additional, Barton, Wiley, additional, O’Sullivan, Orla, additional, Cotter, Paul D., additional, Quilter, Karina, additional, Joyce, Susan A., additional, Murphy, Mike, additional, DunnGalvin, Gillian, additional, Dinan, Timothy G, additional, and Marzorati, Massimo, additional

Published
2021
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73. Development and validation of the simulator of the Canine Intestinal Microbial Ecosystem (SCIME)

Author

Duysburgh, Cindy, Ossieur, Wendy, De Paepe, Kim, Van den Abbeele, Pieter, Vichez-Vargas, Ramiro, Vital, Marius, Pieper, Dietmar H., Van de Wiele, Tom, Hesta, Myriam, Possemiers, Sam, and Marzorati, Massimo

Subjects
ADULT DOGS, validation, FERMENTATION, FOS, PROTEIN CATABOLITES, EFFECTS MODELS, in vitro, METABOLISM, GUT MICROBIOME, TRACT NUTRIENT DIGESTIBILITIES, INULIN, dog, FECAL MICROBIOTA, SCIME, POPULATIONS, Veterinary Sciences
Abstract

Whereas a wide variety of in vitro models have been developed and validated to assess the effect of specific food ingredients on the human gut microbiome, such models have only been developed and applied to a limited extent for companion animals. Since the use of pre- and probiotics to improve gut health is an emerging research topic in the field of companion animals and as dogs are often used as laboratory animals in developing and testing of pharmaceuticals, the current study aimed to establish an adequate canine in vitro model. This consisted of a four-stage reactor composed of a stomach and small intestinal compartment followed by a proximal and distal colon. This semi-continuous gastrointestinal tract model allowed a long-term, region-dependent, and pH-controlled simulation of the colon-associated microbial community of dogs. Upon reaching a functional steady state, the simulated canine microbial community composition proved to be representative of the in vivo situation. Indeed, the predominant bacterial phyla present in the in vitro proximal and distal colon corresponded with the main bacterial phyla detected in the fecal material of the dogs, resulting in an average community composition along the simulated canine gastrointestinal tract of 50.5% Firmicutes, 34.5% Bacteroidetes, 7.4% Fusobacteria, 4.9% Actinobacteria, and 2.7% Proteobacteria. A parallel in vivo-in vitro comparison assessing the effects of fructooligosaccharides (FOS) on the canine microbial community composition showed a consistent stimulation of Lactobacillus concentrations in the in vivo fecal samples as well as in the in vitro canine gut model. Furthermore, the in vitro platform provided additional insights about the prebiotic effect of FOS supplementation of dogs, such as a reduced abundance of Megamonas spp. which are only present in very low abundance in in vivo fecal samples, indicating an interesting application potential of the developed canine in vitro model in research related to gastrointestinal health of dogs.

Published
2020

75. The impact of the Fungus-Host-Microbiota interplay uponCandida albicansinfections: current knowledge and new perspectives

Author

d'Enfert, Christophe, primary, Kaune, Ann-Kristin, additional, Alaban, Leovigildo-Rey, additional, Chakraborty, Sayoni, additional, Cole, Nathaniel, additional, Delavy, Margot, additional, Kosmala, Daria, additional, Marsaux, Benoît, additional, Fróis-Martins, Ricardo, additional, Morelli, Moran, additional, Rosati, Diletta, additional, Valentine, Marisa, additional, Xie, Zixuan, additional, Emritloll, Yoan, additional, Warn, Peter A, additional, Bequet, Frédéric, additional, Bougnoux, Marie-Elisabeth, additional, Bornes, Stephanie, additional, Gresnigt, Mark S, additional, Hube, Bernhard, additional, Jacobsen, Ilse D, additional, Legrand, Mélanie, additional, Leibundgut-Landmann, Salomé, additional, Manichanh, Chaysavanh, additional, Munro, Carol A, additional, Netea, Mihai G, additional, Queiroz, Karla, additional, Roget, Karine, additional, Thomas, Vincent, additional, Thoral, Claudia, additional, Van den Abbeele, Pieter, additional, Walker, Alan W, additional, and Brown, Alistair J P, additional

Published
2020
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96. Yeast-Derived Formulations Are Differentially Fermented by the Canine and Feline Microbiome As Assessed in a Novel In VitroColonic Fermentation Model

Author

Van den Abbeele, Pieter, Moens, Frédéric, Pignataro, Giulia, Schnurr, Judy, Ribecco, Cataldo, Gramenzi, Alessandro, and Marzorati, Massimo

Abstract

The current study evaluated the effect of five yeast-derived formulations (T1–T5) on microbial metabolism and composition of the canine and feline gut microbiota using a novel in vitrocolonic incubation approach. This novel in vitromodel allowed for growth of the entire spectrum of dog- and cat-derived bacteria from the inoculum, thus offering an excellent platform to evaluate effects of nutritional interventions on the gut microbiota. Further, yeast-derived ingredients differentially increased production of acetate, propionate, butyrate, ammonium, and branched short-chain fatty acids, with T5 and T1 consistently stimulating propionate and butyrate, respectively. 16S-targeted Illumina sequencing coupled with flow cytometry provided unprecedented high-resolution quantitative insights in canine and feline microbiota modulation by yeast-derived ingredients, revealing that effects on propionate production were related to Prevotellaceae, Tannerellaceae, Bacteroidaceae, and Veillonellaceae members, while effects on butyrate production were related to Erysipelotrichaceae, Lachnospiraceae, Ruminococcaceae, and Fusobacteriaceae. Overall, these findings strengthen the health-promoting potential of yeast-derived ingredients.

Published
2020
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97. A Bacteriophage Cocktail Eliminates Salmonella Typhimurium from the Human Colonic Microbiome while Preserving Cytokine Signaling and Preventing Attachment to and Invasion of Human Cells by Salmonella In Vitro.

Author

MOYE, ZACHARY D., WOOLSTON, JOELLE, VAN DEN ABBEELE,, PIETER, DUYSBURGH, CINDY, VERSTREPEN, LYNN, DAS, CHYTHANYA RAJANNA, and MARZORATI, MASSIMO

Abstract

Nontyphoidal Salmonella strains continue to be a major cause of foodborne illness globally. One intriguing approach to reducing the risk of salmonellosis is the direct ingestion of phages targeting Salmonella to enhance natural gut resilience and provide protection during foodborne disease outbreaks. We evaluated the ability of a prophylactically administered bacteriophage cocktail, the foodborne outbreak pill (FOP) targeting Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella, to resolve a Salmonella infection in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME), a simulated gut platform populated by the human intestinal microbiome of healthy donors. The FOP preparation eliminated Salmonella enterica serovar Typhimurium from the colon compartment of the SHIME platform but health-associated metabolites, such as short-chain fatty acids and lactate, remained stable or increased in a donor-dependent manner. In studies of human intestinal cells, pretreatment of Salmonella Typhimurium with the FOP cocktail preserved lipopolysaccharide-stimulated signaling in a Caco-2–THP-1 Transwell system and prevented destruction of the Caco-2 monolayer by Salmonella. Adhesion and invasion of intestinal epithelial cells by Salmonella—a critical factor in Salmonella pathogenesis—was blunted when the bacteria were incubated with the FOP preparation before addition to the monolayer. The FOP phage cocktail was effective for (i) eliminating Salmonella from a simulated human gut without disturbing the indigenous microbiota and (ii) reducing the risk of invasion by Salmonella into the intestinal epithelia. These results suggest that the FOP preparation may be of value for reducing the risk of salmonellosis in humans, e.g., during foodborne disease outbreaks. [ABSTRACT FROM AUTHOR]

Published
2019
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99. Arriving on time: Estimating travel time distributions on large-scale road networks

Author

Hunter, Timothy, Hofleitner, Aude, Reilly, Jack, Krichene, Walid, Thai, Jerome, Kouvelas, Anastasios, van den Abbeele, Pieter, and Bayen, Alexandre

Subjects
Inference, Machine learning, Estimation
Abstract

Most optimal routing problems focus on minimizing travel time or distance traveled. Oftentimes, a more useful objective is to maximize the probability of on-time arrival, which requires statistical distributions of travel times, rather than just mean values. We propose a method to estimate travel time distributions on large-scale road networks, using probe vehicle data collected from GPS. We present a framework that works with large input of data, and scales linearly with the size of the network. Leveraging the planar topology of the graph, the method computes efficiently the time correlations between neighboring streets. First, raw probe vehicle traces are compressed into pairs of travel times and number of stops for each traversed road segment using a `stop-and-go' algorithm developed for this work. The compressed data is then used as input for training a path travel time model, which couples a Markov model along with a Gaussian Markov random field. Finally, scalable inference algorithms are developed for obtaining path travel time distributions from the composite MM-GMRF model. We illustrate the accuracy and scalability of our model on a 505,000 road link network spanning the San Francisco Bay Area., Internal Technical Report

Published
2013

100. Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli

Author

Van den Abbeele, Pieter, Roos, Stefaan, Eeckhaut, Venessa, MacKenzie, Donald A, Derde, Melanie, Verstraete, Willy, Marzorati, Massimo, Possemiers, Sam, Vanhoecke, Barbara, Van Immerseel, Filip, and Van de Wiele, Tom

Subjects
FECES, SP NOV, MUCUS, MICROBIAL COMMUNITY, DIVERSITY, food and beverages, Biology and Life Sciences, GASTROINTESTINAL-TRACT, ADHESION, LACTIC-ACID BACTERIA, PROBIOTICS, RHAMNOSUS GG
Abstract

To avoid detrimental interactions with intestinal microbes, the human epithelium is covered with a protective mucus layer that traps host defence molecules. Microbial properties such as adhesion to mucus further result in a unique mucosal microbiota with a great potential to interact with the host. As mucosal microbes are difficult to study in vivo, we incorporated mucin-covered microcosms in a dynamic in vitro gut model, the simulator of the human intestinal microbial ecosystem (SHIME). We assessed the importance of the mucosal environment in this M-SHIME (mucosal-SHIME) for the colonization of lactobacilli, a group for which the mucus binding domain was recently discovered. Whereas the two dominant resident Lactobacilli, Lactobacillus mucosae and Pediococcus acidilactici, were both present in the lumen, L. mucosae was strongly enriched in mucus. As a possible explanation, the gene encoding a mucus binding (mub) protein was detected by PCR in L. mucosae. Also the strongly adherent Lactobacillus rhamnosus GG (LGG) specifically colonized mucus upon inoculation. Short-term assays confirmed the strong mucin-binding of both L. mucosae and LGG compared with P. acidilactici. The mucosal environment also increased long-term colonization of L. mucosae and enhanced its stability upon antibiotic treatment (tetracycline, amoxicillin and ciprofloxacin). Incorporating a mucosal environment thus allowed colonization of specific microbes such as L. mucosae and LGG, in correspondence with the in vivo situation. This may lead to more in vivo-like microbial communities in such dynamic, long-term in vitro simulations and allow the study of the unique mucosal microbiota in health and disease.

Published
2012

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