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Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In the present study, effects of maturity stage on structural characteristics and biosynthesis/hydrolysis-associated genes expression of glucans from Volvariella volvacea fruit body were well investigated. Elongation and pileus expansion stages decreased total soluble carbohydrate and protein contents to 17.09 mg/g and 8.33 mg/g, and significantly accumulated the total amino acids contents to 32.37 mg/g. Yields of crude polysaccharides significantly increased to 8.12% at egg stage and decreased to 3.72% at pileus expansion stage. Purified VVP I-a and VVP I-b were proved to be α-glucans. The maturity process affected the monosaccharide compositions, decreased the molecular weights of VVP I-a and VVP I-b with decreased transcription levels of glucan biosynthesis-associated enzyme genes vvugp and vvgls and increased glucan hydrolysis-associated glucanase gene vvexg2 expression with no significant effects on backbone structures including glycosidic linkages and configurations. The findings would benefit for understanding change patterns of V. volvacea glucan structures and their biosynthesis/hydrolysis-associated genes expression at maturity stages. [ABSTRACT FROM AUTHOR]

Volvariella volvacea has bioactivities in improving immunity, anti‐oxidation, and alleviating obesity, which is an excellent functional food. Polysaccharide from Volvariella volvacea (VPS), one of the main bioactive components, exerts a potential fat‐lowering effect, but its exact mechanism remains unclear. In this study, the effects and molecular pathways of VPS regulate the fat deposition of Caenorhabditis elegans. Results showed that VPS at low (250 μg/ml), medium (500 μg/ml) and high (750 μg/ml) concentrations all reduced the overall fat, without inhibitory effects on the growth and movement abilities of nematode. VPS at 500 μg/ml could dramatically decrease the triglyceride (TG) level of wild‐type nematode, while no significant changes in TG content were observed in mutants deficient in aak‐2 (energy receptor), nhr‐49 (nuclear transcription factor), fat‐5, and fat‐7 genes. VPS declines fat storage of C. elegans, largely through the aak‐2/nhr‐49‐mediated fatty acid synthesis pathway, and partially the acs‐2‐mediated fatty acid oxidation pathway. Practical applications: A model illustrates the mechanism of polysaccharide from Volvariella volvacea (VPS) inhibiting fat accumulation in Caenorhabditis elegans. VPS may directly or indirectly activate the energy sensor aak‐2, which governs lipid metabolism. Results demonstrate that VPS regulates fat metabolism including fatty acid oxidation (FAO) and fatty acid synthesis (FAS), rather than lipolysis. In the FAO, VPS promotes FAO by up‐regulating the mRNA and protein levels of acs‐2. In FAS, VPS significantly down‐regulated the transcriptional regulator nhr‐49 and the downstream targets fat‐5, fat‐6, and fat‐7, thereby declining the overall fat deposition. In conclusion, VPS inhibits the fat accumulation of C. elegans largely dependent on an aak‐2/nhr‐49‐mediated FAS pathway. [ABSTRACT FROM AUTHOR]

Low biological efficiency is a defect in straw mushroom production. Mycelia of the straw mushroom were exposed to UV radiation to induce mutagenesis and improve the strains of straw mushroom for a high yield. A UV exposure time that yielded a survival rate of about 25% was applied for mutation induction. After UV treatment, 65 putative mutant strains were obtained and seven fast-growing strains were selected for further analysis. Mushroom cultivation experiments revealed that the biological efficiency of four of the new strains was approximately 30% higher than the parental strain. The radial mycelial colony growth rate was assessed on different media and under various temperature and pH conditions for physical characterization. The results indicated that the overall growth rates of the mutant strains were higher than the parental strain. Genetic characterization by random amplification of polymorphic DNA analysis revealed that the genome had been altered in the mutant strains.

Volvariella volvacea, with high commercial, nutritional and medicinal value, is widely cultivated in tropical and subtropical regions. The effects of supplementation on mushroom yield has been studied. We showed that the optimal application of sodium acetate (NaAc) was spray application of a 0.08% concentration during the substrate mixing stage which could increase yields by up to 89.16% and enhance the enzymatic hydrolysis of cellulose and hemicellulose from the substrate. For most enzymes tested maximum activity occurred during the fruiting body growth and development stage, which led to degradation of the substrate, increasing the available nutrients for mycelial propagation and fruiting body growth and development. Meanwhile, NaAc also significantly increased the indole-3-acetic acid (IAA) content in the early fruiting body development stage of V. volvacea, It was observed that IAA promotes not only plant primordium differentiation; but also the primordium differentiation of edible fungi. Furthermore, treatments with three acetate salts had an increase of yield by 30.22% on average. The mechanisms by which NaAc application may improve the yield of V. volvacea are discussed.

Bamboo shoot shell (BSS), as agricultural waste, is mostly burned or discarded, causing serious environment pollution. In this study, the degradation and utilization of BSS by the edible fungus Volvariella Volvacea was investigated. The composition of V. volvacea fruit body was determined by HPLC-MS, GC-MS and ICP-OES. The activities of CMCase and xylanase were monitored by DNS (3,5-dinitrosalicylic acid) method. Laccase activity was assayed by the oxidation reaction of ABTS [2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate)]. The degraded bamboo shoot shell powder was characterized by FTIR and SEM. The results showed that the mycelium of V. volvacea could degrade and utilize BSS for growth. The activities of carboxymethyl cellulase and laccase were increased during the cultivation. At the same time, the physical structure of the shell fiber becames porous and rough. Most of the products of decayed fibers contain alkanes, ethyl or methyl groups. Moreover, the biological efficiency (fruiting body yield) of V. volvacea cultivated on BSS was 1.52-fold higher than that of straw cultivation. The contents of total lipid, elaidic acid (C18:1n-9), total essential amino acids, total amino acids and iron in V. volvacea fruit bodies grown on BSS were 1.11, 1.66, 1.52, 1.60 and 1.30-fold higher than those of straw treatment, respectively. This study provides an effective method to solve the environmental pollution caused by BSS, and provides a new way for the potential utilization of BSS in edible fungi cultivation. [ABSTRACT FROM AUTHOR]

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In this study, a novel homogeneous polysaccharide (HVP-1) was purified from the Volvariella volvacea. Its structural characteristics and anti-oxidant activity in vitro were further evaluated. The results revealed that HVP-1 was composed of mannose, glucose, galactose and arabinose in a molar ratio (mol %) of 55.37: 15.74: 25.20: 3.69. Its main chain consisted of →4)- β -D-Gal p -(1→, →6)- α -D-Glc p -(1→, →3)- α -D-Glc p -(1→, →4)- β -D-Man p -(1→ and →3,6)- β -D-Man p -(1→. The branched structure α -L-Ara f -(1→, →2)- β -D-Glc p -(1→ and →6)- β -D-Man p -(1→ were connected to →3,6)- β -D-Man p -(1→ through the O -3 position. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed that HVP-1 had porous sheet-like structure with a triple helix conformation. Anti-oxidant activity experiments showed that HVP-1 alleviated H 2 O 2 -induced oxidative damage by reducing the accumulation of reactive oxygen species, increasing the activity of related enzymes in cells, and activating the Nrf2/HO-1 signaling pathway. These results suggested that HVP-1 had the potential to be used as a natural anti-oxidant in functional foods and pharmaceuticals. • A novel homogeneous polysaccharide HVP-1 was isolated and purified from Volvariella volvacea. • The structure and anti-oxidant activity of HVP-1 were characterized. • HVP-1 could alleviate the accumulation of reactive oxygen species and enhance related enzyme activities. • HVP-1 activated the Nrf2/HO-1 signaling pathway. [ABSTRACT FROM AUTHOR]

Polysaccharides from Volvariella volvacea (VVP) were investigated for their cosmetic-related activities and in vivo efficacy for use as a multifunctional active cosmetic ingredient. Three different polysaccharide extraction methods, including hot water shaking (HS), microwave-assisted (MA) and ultrasonic-assisted (UA), were used. Extractable yield, polysaccharide contents and biological activities, including antioxidant, anti-tyrosinase and anti-elastase activities, were compared. The polysaccharides from HS provided the highest extraction yield (15.58 ± 0.96% w/w) and the highest beta-glucan content (18.80 ± 0.81% w/w). The HS polysaccharides also possessed the highest inhibitory effects toward lipid peroxidation (IC50 of 0.0378 mg/mL), tyrosinase (51.46 mg KAE/g), and elastase (604.21 ± 73.66 mg EGCG/g). The cytotoxicity of the VVP was determined for safe use. A cosmetic gel cream containing VVP was developed and 0.2% VVP formulation was observed to be the most stable in color. UV protection factors, skin irritation by single patch test, and in vivo efficacy, including skin moisturization, anti-wrinkle and whitening, were measured. The VVP showed no cytotoxicity against human dermal skin fibroblast. The gel cream containing VVP provided less sun protection factor; however, it significantly exhibited the skin benefits of increasing moisture, gross elasticity, net elasticity, and skin firmness. Improvements to skin roughness, scaliness, wrinkles and in melanin content were also depicted gradually along 8 weeks. V. volvacea, therefore, could be a good source for polysaccharides being used as a moisturizing, anti-wrinkle, and whitening agent in cosmetic preparations.

Volvariella volvacea, also known as straw mushroom, is an edible mushroom, and a highly nutritious food source. In Malaysia, tremendous increase in the consumption of this mushroom is due to its characteristics; short cropping duration, distinct flavour, and pleasant taste. During harvesting, the maturity of V. volvacea is vital to obtain high quality produce. As the mature stage quickly succeeds the immature stage, farmers usually collect both the immature and mature stages at harvest. Thus, the objective of the present work was to evaluate the physical quality, nutrients, and health risk assessment of V. volvacea at both maturity stages. The mushrooms were cultivated on composted empty fruit bunch (EFB), and harvested after a week at immature (button) and mature (veil opening) stages. The absence of spores at the button stage could lower the metabolic activity after harvest, thus decelerating the deterioration rate. Button stage mushrooms showed significantly higher firmness and higher content of glutamic acid. In term of safety, both stages were within the dietary intake limit for heavy metals. In conclusion, the button stage could be suitable and practical for consumption and commercialisation. [ABSTRACT FROM AUTHOR]

Studies were carried out to investigate cultivation of some indigenous edible mushrooms in Anambra State. The most suitable substrates for cultivation of Pleurotus tuberregium and Volvariella volvacea were also investigated. Spawn of V. volvacea was obtained from the Department of Life Science, University of Benin, Edo State and sclerotia of P. Tuberregium purchased from Eke Awka Market, Anambra State were used for this study. Plantain leaves and oil palm bunch wastes were substrates employed for V. volvacea whereas topsoil was used for P. tuberregium cultivation. The methods used were site preparation, substrate preparation, soil preparation, sclerotia preparation, cultivation of sclerotia, irrigation, spawn cultivation and harvesting of mushrooms. Means were analyzed statistically using (ANOVA) to test for significance. Means were separated using Duncan's Multiple Range Tests (DMRT).The highest number of fruit bodies of V. volvacea, 31±2.68 was obtained from plantain leaves on day 2 after primordial emergence whereas the least, 2±1.00 was recorded from oil palm bunch wastes on days 6 and 14, respectively. The highest number of P. tuberregium, 6±1.58 was recorded on day 12 after primordial emergence while the least, 2±0.49 was recorded on days 7, 13 and 14, respectively. All the three substrates utilized for cultivation of mushrooms in this study supported their growth and development. Plantain leaves supported fast colonization and produced high yield fruit bodies of V. volvacea. The ability to use agricultural wastes for cultivation of mushrooms will boost food production for ever increasing population. [ABSTRACT FROM AUTHOR]

In Volvariella volvacea, an important species of edible mushroom, cryogenic autolysis is a typical phenomenon that occurs during abnormal metabolism. Analysis of gene expression profiling and qPCR showed that chilling stress (CS) significantly and continuously upregulated only one type of Argonaute in V. volvacea, i.e., VvAgo1. Structural and evolutionary analysis revealed that VvAgo1 belongs to the Ago-like family, and its evolution has involved gene duplication, subsequent gene loss, and purifying selection. Analysis of its interaction network and expression suggested that CS triggers VvAgo1-mediated miRNA-like RNA (milRNA) biogenesis in V. volvacea V23 but not in VH3 (a composite mutant strain from V23 with improved CS resistance). Small RNA sequencing and qPCR analysis confirmed that CS triggered the increased milRNA expression in V23 and not in VH3. The predicted target genes of the increased milRNAs were enriched in several pathways, such as signal transduction and ubiquitination. Heatmap analysis showed that CS altered the expression profile of milRNAs with their target genes related to signal transduction and ubiquitination in V23. Combined analysis of transcriptome and proteome data confirmed that most of the target genes of the increased milRNAs were not translated into proteins. Our observations indicate that CS might trigger VvAgo1-mediated RNAi to facilitate the cryogenic autolysis of V. volvacea.

Copyright of Acta Edulis Fungi is the property of Acta Edulis Fungi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Edulis Fungi is the property of Acta Edulis Fungi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Edulis Fungi is the property of Acta Edulis Fungi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In Volvariella volvacea , an important species of edible mushroom, cryogenic autolysis is a typical phenomenon that occurs during abnormal metabolism. Analysis of gene expression profiling and qPCR showed that chilling stress (CS) significantly and continuously upregulated only one type of Argonaute in V. volvacea , i.e., VvAgo1. Structural and evolutionary analysis revealed that VvAgo1 belongs to the Ago-like family, and its evolution has involved gene duplication, subsequent gene loss, and purifying selection. Analysis of its interaction network and expression suggested that CS triggers VvAgo1-mediated miRNA-like RNA (milRNA) biogenesis in V. volvacea V23 but not in VH3 (a composite mutant strain from V23 with improved CS resistance). Small RNA sequencing and qPCR analysis confirmed that CS triggered the increased milRNA expression in V23 and not in VH3. The predicted target genes of the increased milRNAs were enriched in several pathways, such as signal transduction and ubiquitination. Heatmap analysis showed that CS altered the expression profile of milRNAs with their target genes related to signal transduction and ubiquitination in V23. Combined analysis of transcriptome and proteome data confirmed that most of the target genes of the increased milRNAs were not translated into proteins. Our observations indicate that CS might trigger VvAgo1-mediated RNAi to facilitate the cryogenic autolysis of V. volvacea. [ABSTRACT FROM AUTHOR]

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

It was hypothesized that white-rot fungus fermented with rice straw and purple field corn improves nutrient utilization via enhanced digestibility and lowers methane (CH4) production due to the effects of the lovastatin compound. The aim of the current experiment was to investigate the effect of inoculation of two fungi belonging to white-rot fungus type on feed value and ruminal fermentation characteristic. The experiment was carried out according to a completely randomized 3 × 3 factorial design: three roughage sources (rice straw, purple corn stover, and purple corn field cob) for three inoculation methods (untreated, P. ostreatus treated, and V. volvacea treated). The two fungi increased concentration of lovastatin when compared to the untreated, and P. ostreatus had higher lovastatin production potential than V. volvacea (p < 0.05). The yield of lovastatin was obtained from rice straw fermentation with P. ostreatus. The monomeric anthocyanin content (MAC) in untreated purple field corn cobs was higher than in the fermentation groups. Ruminal fermentation gas production from soluble fractions ranged from −2.47 to 1.14 and differed among the treatments (p < 0.01). In comparison to all treatments, the gas production rate for the insoluble fraction was significantly highest (p < 0.01) in treatment alone, in which purple field corn stover was fermented with P. ostreatus and V. volvacea. There was significant interaction in in vitro dry matter digestibility at 12 h of incubation. Purple field corn cob had a higher significant effect on in vitro DM digestibility at 12 and 24 h after incubation when compared to that of other groups. Moreover, current research has found that roughage fermented with P. ostreatus and V. volvacea increased in vitro DM digestibility at 24 h after incubation. Fermenting roughage with fungi did not affect rumen pH, which ranged from 6.60 to 6.91 (p > 0.05), while P. ostreatus resulted in increased levels of ruminal ammonia-nitrogen concentrations. Propionic acid increased in all roughages fermented with P. ostreatus or V. volvacea after 8 h of ruminal fermentation testing. The two fungi fermented as substrate treatments had significantly lower (p < 0.05) CH4 production. Based on the improved rumen DM digestibility and reduced CH4 production, P. ostreatus and V. volvacea could be utilized for enhancing feeding efficiency of roughage. [ABSTRACT FROM AUTHOR]

Phytochemical investigation of the fruiting body of Volvariella volvacea led to the isolation of a new furanone, 2(5H)-furanone-4-propionic acid named volvafuranone A (1), together with twelve known compounds (2–13). Compounds 2–7, 9–11 were isolated from this mushroom for the first time. The isolated compounds were assessed for their cytotoxicity against four human tumour lines (SGC-7901, PC-3M, MCF-7, HepG-2), and the results showed that compound 2, 3, 12, 13 have significant cytotoxicity with IC50 values of 5.90 μM (HepG-2), 20.72 μM (HepG-2), 27.98 μM (PC-3M) and 23.15 μM (PC-3M), respectively. [ABSTRACT FROM AUTHOR]

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Mushrooms are health foods due to their nutritional values and antioxidant properties. This study investigated chemical properties, total phenolic content (TPC), antioxidant activities (2, 2-diphenyl-1-picrylhydrazyl; DPPH and 2, 2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid); ABTS) and selected phenolic acids from five different tray-dried and freeze-dried mushrooms (Pleurotus ostreatus, Pleurotus pulmonarius, Schizophyllum commune, Volvariella volvacea and Lentinus edodes). The extracts were prepared using methanol and hot water extractions. The results revealed that V. volvacea had the highest protein (28.70%) and ash (9.48%) contents. There were significant differences among some tray dried and freeze dried mushrooms regarding TPC, DPPH and ABTS. However, tray drying tended to improve TPC, DPPH and ABTS in P. ostreatus, P. pulmonarius and L. edodes. Among five mushroom species, both methanol and hot water extractions of V. volvacea had the highest TPC, DPPH and ABTS values but there were no significant differences between mushrooms prepared using tray drying and freeze drying. Moreover, the yield and contents of gallic acid and p-hydroxybenzoic acid from the hot water extracts were higher than from the methanol extracts. Therefore, tray-dried V. volvacea had the most potential as a natural antioxidant and could be used as a functional ingredient in healthy food products. [ABSTRACT FROM AUTHOR]

The straw-rotting edible fungus Volvariella volvacea is a widely cultivated edible fungus across China and Southeast Asian countries. Three complete mitochondrial genomes of V. volvacea from China, Thailand, and India were determined using the next-generation sequencing technology. The genome sizes of the three strains (China, Thailand, and India) were 62,541 bp, 64,531 bp, and 65,668 bp with GC contents of 38.46%, 38.56%, and 38.52%, respectively. All the genomes encoded 14 conserved protein-coding genes, the small ribosomal RNA subunits (rns), large ribosomal RNA subunits (rnl), and 23 tRNAs were located on the same strand. In the putative protein-coding genes, four introns were distributed in cox1 in the genomes of V23-1 and V8. 5 introns (four introns invaded into cox1and one intron invaded into cob) were detected in Tai8. The phylogenetic analysis confirmed that V. volvacea was a number of Agaricales. This mitochondrial genome may open new avenues for understanding the phylogeny and evolution of Pluteaceae and Agaricales.

Volvariella volvacea is a typical edible Basidiomycete with a high-temperature tolerance. It has a strong fibrinolysis capability and consumes abundant agricultural wastes. In agricultural cultivation, mycelial subculturing has been adopted, leading to serious strain degeneration. In this study, continuous mycelial subculturing of the common V. volvacea strain V971 (original strain recorded as M0) was performed in potato dextrose agar (PDA) medium. One generation of the strain was preserved every 3 months (90 days); thus, six generations of degenerated strains (M1–M6) were obtained after 18 months of mycelial subculturing. The original and degenerated strains were preserved in sterile paraffin liquid at room temperature (18–25°C). The biological traits and nutrients of M0 and M1–M6 were studied. The mycelial growth rate and biomass initially increased and then decreased as the degeneration progressed, reaching minimum levels of 0.041 ± 0.001 cm/h and 1.82 ± 0.25 g, respectively, at M6. Additionally, the polysaccharide, protein, polyphenol, flavone, total amino acid, and total mineral element contents of the strains decreased continuously, reaching minimum levels of 30.12 ± 3.12 g/100 g, 26.42 ± 2.1 g/100 g, 1.08 ± 0.05 g/100 g, 4.23 ± 0.21 g/100 g, 12.51 mg/g, and 398.05 mg/kg, respectively, at M6. The decolorization capability of V. volvacea in liquid medium supplemented with bromothymol blue and lactose reflected the degree of strain degeneration, with the capability weakening as the degeneration intensified. These results are highly significant for V. volvacea production. The mycelial characteristics during subculture-associated degeneration were described and provide an early identification method for V. volvacea’s degeneration.

• Dihydro-β-ionone was first identified in Volvariella volvacea. • Odor activity values of main constituents of Volvariella volvacea were determined. • SAFE was enriched in aroma-active components for Volvariella volvacea aroma analysis. The volatile compounds of Volvariella volvacea mushroom were investigated by solvent assisted flavor evaporation (SAFE)/gas chromatography-mass spectrometry (GC–MS), gas chromatography-olfactometry (GC-O), odor activity value (OAV), combined with aroma reconstitution and omission. The results showed that a total of 63 compounds were detected after SAFE extraction. A total of 26 compounds were determined after GC-O and 17 compounds whose OAV greater than 1 were subjected to reconstitution and omission experiments. The results showed that dihydro-β-ionone, 1-octen-3-one, 1-octen-3-ol, γ-undecalactone, 3-octanol, 2-octanone, hexanal, 2-methylbutanal, camphene, carvone, 2-nonanone, and phenylacetaldehyde have been successfully identified as the key aroma compounds. More significantly, dihydro-β-ionone as a key aroma compound was first found in Volvariella volvacea mushroom. [ABSTRACT FROM AUTHOR]

Volvariella volvacea is a typical edible Basidiomycete with a high-temperature tolerance. It has a strong fibrinolysis capability and consumes abundant agricultural wastes. In agricultural cultivation, mycelial subculturing has been adopted, leading to serious strain degeneration. In this study, continuous mycelial subculturing of the common V. volvacea strain V971 (original strain recorded as M0) was performed in potato dextrose agar (PDA) medium. One generation of the strain was preserved every 3 months (90 days); thus, six generations of degenerated strains (M1–M6) were obtained after 18 months of mycelial subculturing. The original and degenerated strains were preserved in sterile paraffin liquid at room temperature (18–25°C). The biological traits and nutrients of M0 and M1–M6 were studied. The mycelial growth rate and biomass initially increased and then decreased as the degeneration progressed, reaching minimum levels of 0.041 ± 0.001 cm/h and 1.82 ± 0.25 g, respectively, at M6. Additionally, the polysaccharide, protein, polyphenol, flavone, total amino acid, and total mineral element contents of the strains decreased continuously, reaching minimum levels of 30.12 ± 3.12 g/100 g, 26.42 ± 2.1 g/100 g, 1.08 ± 0.05 g/100 g, 4.23 ± 0.21 g/100 g, 12.51 mg/g, and 398.05 mg/kg, respectively, at M6. The decolorization capability of V. volvacea in liquid medium supplemented with bromothymol blue and lactose reflected the degree of strain degeneration, with the capability weakening as the degeneration intensified. These results are highly significant for V. volvacea production. The mycelial characteristics during subculture-associated degeneration were described and provide an early identification method for V. volvacea 's degeneration. [ABSTRACT FROM AUTHOR]

• A series of separation techniques guided by sensory evaluation were used. • Two peptides with umami taste were identified in Volvariella volvacea. • Taste characteristics and dose-response relationships of peptides were studied. Mushroom peptides produced by high-pressure cooking from Volvariella volvacea were found to retain and enhance the umami taste. Three tasty peptides were identified as Ala-Ser-Asn-Met-Ser-Asp-Leu (ASNMSDL), Tyr-Tyr-Gly-Ser-Asn-Ser-Ala (YYGSNSA) and Leu-Gln-Pro-Leu-Asn-Ala-His (LQPLNAH) by UPLC-Q-TOF-MS, after a series of separation methods of ultrafiltration, gel filtration chromatography and reverse phase high-performance liquid chromatography. Each fractionation step is based on the results of sensory evaluation. ASNMSDL and LQPLNAH have umami taste with threshold values of 10.19 and 12.63 mmol/L, respectively. In addition, these two peptides also showed umami-enhancing properties with a threshold estimated at 13.58 and 18.95 mmol/L, respectively. Furthermore, the peptides showed better sensory taste than mixtures of their constituent amino acids. After separation and purification, the content of taste peptides in Volvariella volvacea is about 1.4%. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Volvariella volvacea (Family: Plutaceae), also more commonly known as paddy straw mushroom, is an edible mushroom with high nutritional content. It is usually cultivated using lignocellulosic-based materials for enhanced production. However, V. volvacea is highly perishable and easily deteriorates in terms of quality and appearance after harvest. The present paper thus aimed to provide a critical review on aspects related to the production of V. volvacea using palm oil empty fruit bunch as cultivation substrate. The different stages of V. volvacea development are also highlighted. The present review also provides some information on the preservation techniques and appropriate postharvest management in extending V. volvacea shelf life to further boost the paddy straw mushroom industry. [ABSTRACT FROM AUTHOR]

Aims: Paddy straw is known to have lignocellulosic materials such as cellulose and hemicellulose which can be readily converted into fermentable sugar for production of bioethanol via simultaneous saccharification and fermentation (SSF). In order to produce ethanol competently, the degradation of biomass by cellulase and highly ethanol-producing microorganism in fermentation process are necessarily needed. However, there is lacking in cellulose degrading organism in producing adequate amount of lignocellulosic enzyme. Therefore, the screening and selection for the best fungi to hydrolyze the lignocellulosic materials as well as forming consortium between two species of fungi has become the main focus. Methodology and results: Thirteen strains of fast-growing fungi were tested qualitatively for cellulase (congo red staining) and polyphenol oxidase (Bavendamm test). All tested strains displayed lignocellulolytic fungi characteristics. The selection was narrowed down by quantitative assay on endoglucanase, exoglucanase, β-glucosidase and xylanase and the highest cellulases enzyme producer were Trichoderma asperellum B1581 (3.93 U/mL endoglucanase; 2.37 U/mL exoglucanase; 3.00 IU/mL β-glucosidase; 54.87 U/mL xylanase), followed by Aspergillus niger B2484 (5.60 U/mL endoglucanase; 1.08 U/mL exoglucanase; 1.57 IU/mL β-glucosidase; 56.85 U/mL xylanase). In compatibility test, both T. asperellum B1581 and A. niger B2484 were inoculated on the same Petri dish for 4 days and the interaction showed by the two species was mutual intermingling. Conclusions, significance and impact of study: Both T. asperellum B1581 and A. niger B2484 produced the highest cellulase enzyme. Since both strains can co-exist and produce enzymes that complete each other, a fungal consortium was suggested to increase the yield of sugars in saccharification process. [ABSTRACT FROM AUTHOR]

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Abstract This study was conducted to develop and optimise an alternative fermentation model system using mushrooms (Pleurotus pulmonarius and Volvariella volvacea) and yeast (Saccharomyces cerevisiae) as the starter cultures, while standard glucose solution was used as the substrate for fermentation. The optimisation was performed on two factors, fermentation time and culture ratio, using D-optimal mixture design. Both fermentation time and culture ratio showed significant (p < 0.05) effects on reducing sugar content and increasing the ethanol content for alcoholic fermentation. The optimum fermentation conditions were 72 h of fermentation and using a S. cerevisiae : P. pulmonarius mycelia: V. volvacea mycelia culture ratio of 1:1:1, achieving a desirability of 0.901. A verification test was performed, and there was no significant difference (p > 0.05) in the ethanol content between the experimental value (13.45 ± 0.99%) and the predicted value (13.52%). Vinegar production was carried out using the optimised alcoholic sample through the inoculation of mother of vinegar and Kombucha. The concentrations of acetic acid from both samples of vinegar were between 2.05% and 2.41% with zero residual ethanol. In conclusion, an alternative fermentation model system for vinegar production using mushrooms and yeast, followed by mother of vinegar and Kombucha inoculation, has been successfully developed with the desired physiochemical properties. Highlights • Alternative fermentation system using mushrooms and yeasts as a starter cultures. • P. pulmonarius and V. volvacea mycelia was used with glucose solution as substrate. • Optimisation on culture ratio and time was carried out using mixture design. • Alcoholic fermentation was optimum at 72 h and culture ratio of 1:1:1. • Vinegar was produced using mother of vinegar and kombucha. [ABSTRACT FROM AUTHOR]

The effects of paper containing different concentrations of 1‐methylcyclopropene (1‐MCP) on the quality of Chinese mushrooms were investigated. Mushrooms were first stored with paper containing 0, 0.25, 0.50, 0.75, 1.00, or 1.25 μl L−1 1‐MCP at 15 ± 1°C for 12 hr and then continued for up to 8 days. 1‐MCP reduced the respiratory peak, weight loss, degree of cap browning, relative leakage rate, and soluble solid content, and delayed the change in the surface hue angle. Mushrooms exposed to paper containing 1‐MCP maintained firmness and sensory attributes. The storage time of Chinese mushrooms treated with 1‐MCP at the best concentration (0.75 μl L−1) was 8 days, which was 4 days longer than that of the control. Browning in mushrooms was related to the vitamin C content, total phenol content, and polyphenol oxidase activity. The results would be very useful to the mushroom industry and provide a theoretical basis for the potential researches of the mechanisms maturation and senescence in Chinese mushrooms. The results in this article could provide a theoretical basis for the potential use of paper containing 1‐MCP in the development of Chinese mushroom commercial production. [ABSTRACT FROM AUTHOR]

Abstract A number of homeobox transcription factors (TFs) play critical role in regulating developmental processes of fungi. However, studies on TFs in fruiting body development of mushroom forming species, Volvariella volvacea , are still at initial stage. Here, we report homeobox TFs in the whole-genomic sequence of V. volvacea and expression analysis of the homeobox TFs during a series of developmental stages. Homeobox TFs were identified using InterPro terms and Fungal Transcription Factor Database (FTFD) from the genome of V. volvacea and quantitative real-time PCR were used for gene expression analysis. Based on phylogenetic analysis, the homeobox TFs of V. volvacea were divided into two groups and showed close relationships with the TFs of other Basidiomycetes. Eight differentially expressed homeobox TFs were selected by digital gene expression analysis from 47 putative homeobox TFs , including five up-regulated genes in primordia and three down-regulated genes in fruiting elongation stage of V. volvacea. VvHox1 , VvHox2 , and VvHox3 might be participating in fruiting body elongation. It can be assumed that VvHox3 might be involved in volva development. Moreover, five TFs (VvHox4-VvHox8) might be contributing in primordia formation. Results indicated that differentially expressed homeobox TFs are significant candidates for fruiting body development study in V. volvacea. Highlights • 214 putative TFs were identified from the genome of Volvariella volvacea. • Eight differentially expressed TFs were obtained from 47 putative homeobox TFs. • Homeobox TFs could be involved in morphological variation in V. volvacea. [ABSTRACT FROM AUTHOR]

A study was conducted to analyze the suitability of local substrates like maize stalk, arecanut husk, coconut leaf stalk and bunch waste for production of oyster mushroom. Quickest spawn run was observed in 22.5 days and first crop was harvested in 27.0 days in the maize stalk substrate. Further, the maximum yield of 297 gm per cover was recorded in maize stalk substrate followed by 277 gm per cover of paddy straw. Highest biological efficiency of 84.79 per cent was also observed in maize stalk followed by 55.40 per cent in paddy straw. Maize stalk as substrate required 36 man hours of labour to produce 100 kg of oyster mushroom and the production cost incurred was Rs. 3,246 per 100 kg. The net returns from maize stalk as substrate was Rs. 6,751 per 100 kg of mushroom and BC ratio was 3.09 which were highest among all the substrates. Paddy straw performance was also good with BC ratio of 2.72. [ABSTRACT FROM AUTHOR]

Abstract Endoglucanase 1 (EG1) isolated from the straw mushroom has great potential in the textile and paper industries. Improving EG1 expression level will add to its value for industrial applications. In this study, we employed two combined strategies to enhance the expression quantity of EG1, which are increase the copy number of EG1 and enhance the folding and secretion efficiency of EG1 in the endoplasmic reticulum by overexpress HAC1. Multiple plasmids, which contains four copies of EG1, were constructed by isocaudamers, resulted a recombinant strain with EG1 activity up to 39.6 U/mL, 262% higher than that measured in the strain containing only a single copy. A significant increase in activity (151%) was found when eight copies of EG1 was introduced into a different host, compared with a host harboring four copies. Further overexpression of the HAC1 transcription factor in the host harboring eight EG1 copies led to activity of 91.9 U/mL, which is 619% higher than that measured in the original strain. Finally, EG1 activity of 650.1 U/mL was achieved in a 3-L scaled–up fed-batch fermenter and the protein yield was 4.05 g/L. The characteristics of recombinant EG1 were also investigated, the optimal values for enzyme activity were 60 °C and pH 5.0, which yielded a catalytic efficiency of 312.9 mL mg−1min−1 using carboxymethyl cellulose(CMC) as the substrate. Highlights • Multi-copy expression strategy and over-expression transcription factor HAC1 could enhance folding and secretion of the recombinant Endoglucanase 1 in Pichia pastoris. • Highest yield and activity of Endoglucanase 1 from the fungus Volvariella volvacea at present. [ABSTRACT FROM AUTHOR]

Abstract The aim of this work was to evaluate the quality and sensory properties of Cantonese sausages incorporated with dried straw mushrooms. Five types of sausage with the addition of 0%, 1%, 2%, 3%, and 4% straw mushrooms were prepared, and their physicochemical traits and sensory acceptance were investigated. The results showed that incorporation of straw mushrooms improved the physical properties, significantly decreased peroxide value, increased the amount of amino acids and volatile compounds, and introduced new volatiles (aldehyde and ester compounds) to Cantonese sausages. Compared to sausages without addition of straw mushrooms, the amount of essential amino acids increased 8-fold, the P/S ratio of fatty acid increased to 0.46 similar to the recommended nutritional value, and the lipid peroxide value reduced 10-fold in Cantonese sausages with addition of 4% straw mushrooms. These results indicate that incorporation of straw mushrooms could be an efficient way to obtain nutritional and healthy Cantonese sausages. Highlights • Effect of straw mushroom on physiochemical and sensorial properties were evaluated. • The straw mushroom improved the flavor and overall quality of Cantonese sausages. • The peroxide value was reduced in the sausages incorporated with straw mushroom. • The straw mushroom enhanced the amount of amino acids and volatile compounds in Cantonese sausage. • Cantonese sausage containing 2% of straw mushroom scored best for general acceptability. [ABSTRACT FROM AUTHOR]

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Mushrooms have been accepted as nutraceutical foods because of their high nutritional and functional values. They have also gained interest due to their medicinal properties, economic importance, and organoleptic merit. In this study, wild Ganoderma lucidum and four commercial mushrooms, that is, Pleurotus ostreatus, Volvariella volvacea, Hericium erinaceus, and Lentinus edodes from Pakistan were screened for their biological activities such as anticancer, antityrosinase, anti‐α‐glucosidase, and antithrombotic activities from their methanol, ethanol, and water extracts. Enzyme inhibition assay showed that selected mushrooms are potent inhibitors with %age inhibition ranging from 19.00% to 80.91%, and 32.85% to 83.38% for tyrosinase and α‐glucosidase, respectively. The best tyrosinase inhibition was shown by P. ostreatus whereas L. edodes was found best as α‐glucosidase inhibitor. These mushrooms were tested against cancer cell lines including HT‐29 colon and H‐1299 lungs carcinoma cell lines. G. lucidum showed 29% and 24% viability of cells against HT‐29 and H‐1299 cell lines, respectively. This antiproliferative effect was in dose‐dependent manner, and the maximum inhibition was observed at 200 μg/ml. Mushrooms extracts were also found effective against clot lysis. The percentage of clot lysis was in the range of 27%–29%. The research would provide knowledge to the people of Pakistan about the importance of locally available commercial mushrooms and wild mushrooms for health improvement and prevention against different kinds of diseases. The research would provide knowledge to the people of Pakistan about the importance of locally available commercial mushrooms and wild mushrooms for health improvement and prevention against different kinds of diseases. [ABSTRACT FROM AUTHOR]

Greenhouse summer squash is a major type of greenhouse vegetable in Shandong Province. In summer, there is a three-month idle period of greenhouse summer squash. Volvariella volvacea is a high temperature resistant high-quality edible fungus. In practice, it is able to use the spent mushroom substrate of Flammulina velutipes to cultivate V. volvacea. In the idle period of greenhouse summer squash, cultivating V. volvacea using the spent mushroom substrate of F. velutipes has high economic and environmental benefits. The cultivation techniques mainly include the preparation of cultivation materials, preliminary preparation for the greenhouse, planting management of V. volvacea, and returning the spent mushroom substrate to the field. By comparison with the conventional summer squash planting, this technique has higher economic benefits and its application prospects will be broad. [ABSTRACT FROM AUTHOR]

The edible straw mushroom Volvariella volvacea has a desirable taste and diverse effects on health; however, it undergoes rapid autolysis at 4 °C. Spray treatment with the β-glucosidase inhibitor DGlucono-1,5-lactone (DGase) at 100 µmol/L maintained the quality of V. volvacea during storage at 4 °C for 72 h. Enhanced alkaline protease and neutral protease activities can act as indicators of cold stress resistance (CSR) in V. volvacea , as well as the stable ATP level. The total sugar and amino acid content is negatively correlated with CSR in V. volvacea. DGase treatment enhanced the CSR of V. volvacea by inducing the overexpression of genes in the mitochondrial ribosome (mitoribosome). Overexpression of a serine/threonine-protein kinase gene in V. volvacea (VSKM1), which promotes mitoribosome activity in yeast, enhanced freezing tolerance. Our findings indicated that overexpression of VSKM1 promoted CSR by enhancing mitoribosome activity for the quality retention of postharvest V. volvacea. • DGlucono-1,5-lactone spraying maintained the long-term quality of V. volvacea at 4 °C. • Physiological indicators of cold stress resistance were identified in V. volvacea. • The enhanced mitoribosome activity promoted cold stress resistance in V. volvacea. • The overexpression of VSKM1 enhanced mitoribosome activity in V. volvacea. [ABSTRACT FROM AUTHOR]

Volvariella volvacea is an important crop in Southeast Asia, but erratic fruiting presents a serious challenge for its production and breeding. Efforts to explain inconsistent fruiting have been complicated by the multinucleate nature, typical lack of clamp connections, and an incompletely identified sexual reproductive system. In this study, we addressed the life cycle of V. volvacea using whole genome sequencing, cloning of MAT loci, karyotyping of spores, and fruiting assays. Microscopy analysis of spores had previously indicated the possible coexistence of heterothallic and homothallic life cycles. Our analysis of the MAT loci showed that only MAT-A, and not MAT-B, controlled heterokaryotization. Thus, the heterothallic life cycle was bipolar. Karyotyping of single spore isolates (SSIs) using molecular markers supported the existence of heterokaryotic spores. However, most SSIs were clearly not heterokaryotic, yet contained structural variation (SV) markers relating to both alleles of both parents. Heterokaryons from crossed, self-sterile homokaryons could produce fruiting bodies, agreeing with bipolar heterothallism. Meanwhile, some SSIs with two different MAT-A loci also produced fruiting bodies, which supported secondary homothallism. Next, SSIs that clearly contained only one MAT-A locus (homothallism) were also able to fruit, demonstrating that self-fertile SSIs were not, per definition, secondary homothallic, and that a third life cycle or genetic mechanism must exist. Finally, recombination between SV markers was normal, yet 10 out of 24 SV markers showed 1:2 or 1:3 distributions in the spores, and large numbers of SSIs contained doubled SV markers. This indicated selfish genes, and possibly partial aneuploidy.

Volvariella volvacea, usually harvested in its egg stage, is one of the most popular mushrooms in Asia. The rapid transition from the egg stage to elongation stage, during which the stipe stretches to almost full length leads to the opening of the cap and rupture of the universal veil, and is considered to be one of the main factors that negatively impacts the yield and value of V. volvacea. Stipe elongation is a common phenomenon in mushrooms; however, the mechanisms, genes and regulation involved in stipe elongation are still poorly understood. In order to study the genes related to the stipe elongation, we analyzed the transcription of laccase genes in stipe tissue of V. volvacea, as some laccases have been suggested to be involved in stipe elongation in Flammulina velutipes. Based on transcription patterns, the expression of Vvlcc3 was found to be the highest among the 11 laccase genes. Moreover, phylogenetic analysis showed that VvLCC3 has a high degree of identity with other basidiomycete laccases. Therefore, we selected and cloned a laccase gene, named Vvlcc3, a cDNA from V. volvacea, and expressed the cDNA in Pichia pastoris. The presence of the laccase signature L1-L4 on the deduced protein sequence indicates that the gene encodes a laccase. Phylogenetic analysis showed that VvLCC3 clusters with Coprinopsis cinerea laccases. The ability to catalyze ABTS (2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) oxidation proved that the product of the Vvlcc3 gene was a functional laccase. We also found that the expression of the Vvlcc3 gene in V. volvacea increased during button stage to the elongation stage; it reached its peak in the elongation stage, and then decreased in the maturation stage, which was similar to the trend in the expression of Fv-lac3 and Fv-lac5 in F. velutipes stipe tissue. The similar trend in expression level of these laccase genes of F. velutipes suggested that this gene could be involved in stipe elongation in V. volvacea.

Molecular markers and genetic maps are useful tools in genetic studies. Novel molecular markers and their applications have been developed in recent years. With the recent advancements in sequencing technology, the genomic sequences of an increasingly great number of fungi have become available. A novel type of molecular marker was developed to construct the first reported linkage map of the edible and economically important basidiomycete Volvariella volvacea by using 104 structural variation (SV) markers that are based on the genomic sequences. Because of the special and simple life cycle in basidiomycete, SV markers can be effectively developed by genomic comparison and tested in single spore isolates (SSIs). This stable, convenient and rapidly developed marker may assist in the construction of genetic maps and facilitate genomic research for other species of fungi.

An examination of the process of extracting crude polysaccharides from Volvariella volvacea solely through hot water treatment (HWE) at 60, 80, and 100 °C and through an approach involving high pressure processing (HPP) at 200, 400, and 600 MPa followed by HWE. The physiological properties of the polysaccharides could be explained by the structural analysis performed via FT-IR spectroscopy and NMR spectroscopy, which revealed the extract composition of the protein-bound polysaccharides connected by β-glycosidic bonds. Under the extraction conditions investigated in this current study, the recommended extraction condition was a combination of HPP (600 MPa, 10 min) and HWE (60 °C, 2 h). This condition gave high crude polysaccharide yields (with a 2-12% increase), and β-glucan content (with a 15-20% increase) without disrupting the β-glycosidic bond, as compared to using HWE alone. High pressure extraction could be an alternative technique for reduced extraction temperatures of active compounds from mushrooms., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Author(s).)

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Copyright of Acta Edulis Fungi is the property of Acta Edulis Fungi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Mechanization of agriculture in the developed and developing countries generates lot of secondary agricultural waste. Efforts are being carried out to use such waste in production of energy, fuel and platform chemicals. Such technologies/processes would have two fold benefits. First, they are based on utilization of waste and thereby providing protection to the environment. Second, they produce value added products from waste and thereby providing additional income source. In this regard, rice is one the highly cultivated crops across the world and therefore generates huge waste in terms of straw. Hence, rice/paddy straw was used as a raw material in a direct process of simultaneous clean separation of small sized nano-silica and lignin. Since paddy straw is available in abundance, low in cost and causes environmental pollution upon burning, its use in the recovery of precious molecules of industrial applications would add value to the economic gain. In the present study, a simple acid-base hydrolytic method was used to recover pure silica (SiO 2 ) nanoparticles and lignin from paddy straw residues, with negligible mineral contaminants. The physio-chemical characteristics of lignin and nano-silica were determined using fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffraction (XRD), transmission electron microscopy (TEM) and scanning electron microscopy (SEM) studies along with energy-dispersive X-ray spectroscopy (EDS) analysis. Highly pure nano-silica and lignin were derived by acid precipitation process at an overall yield of 9.26% and 2.30%, respectively. The elemental composition of SiO 2 and lignin was authenticated by FTIR pattern and EDS analysis. Both SiO 2 and lignin were found to be amorphous in nature as confirmed by XRD investigation. Through this direct method, uniform sized spherical nano-silica particles of around 17 nm have been separated from paddy straw. This process perfectly worked at kilogram scale of paddy waste with similar equivalent level of yield. Hence, the recovery of such commercially important molecules would not only provide the possible protection to environmental pollution due to the abundance of paddy straw and its burning but can also contribute to the sustainable economic growth of the country by providing additional income to the farmers. [ABSTRACT FROM AUTHOR]