Search

Your search keyword '"Urbach, Serge"' showing total 288 results
Start Over Author "Urbach, Serge"
288 results on '"Urbach, Serge"'

52. Identification of Differential Responses of Goat PBMCs to PPRV Virulence Using a Multi-Omics Approach

Author

Eloiflin, Roger-Junior, primary, Auray, Gaël, additional, Python, Sylvie, additional, Rodrigues, Valérie, additional, Seveno, Martial, additional, Urbach, Serge, additional, El Koulali, Khadija, additional, Holzmuller, Philippe, additional, Totte, Philippe, additional, Libeau, Genevieve, additional, Bataille, Arnaud, additional, and Summerfield, Artur, additional

Published
2021
Full Text
View/download PDF

54. Identification of differential responses of goat PBMCs to PPRV virulence using a multi-omics approach

Author

Eloiflin, Roger-Junior, Auray, Gaël, Python, Valérie, Rodrigues, Valérie, Seveno, Martial, Urbach, Serge, El Koulali, Khadija, Holzmuller, Philippe, Totté, Philippe, Libeau, Geneviève, Bataille, Arnaud, Summerfield, Artur, Eloiflin, Roger-Junior, Auray, Gaël, Python, Valérie, Rodrigues, Valérie, Seveno, Martial, Urbach, Serge, El Koulali, Khadija, Holzmuller, Philippe, Totté, Philippe, Libeau, Geneviève, Bataille, Arnaud, and Summerfield, Artur

Abstract

Peste des petits ruminants (PPR) is an acute transboundary infectious viral disease of small ruminants, mainly sheep and goats. Host susceptibility varies considerably depending on the PPR virus (PPRV) strain, the host species and breed. The effect of strains with different levels of virulence on the modulation of the immune system has not been thoroughly compared in an experimental setting so far. In this study, we used a multi-omics approach to investigate the host cellular factors involved in different infection phenotypes. Peripheral blood mononuclear cells (PBMCs) from Saanen goats were activated with a T-cell mitogen and infected with PPRV strains of different virulence: Morocco 2008 (high virulence), Ivory Coast 1989 (low virulence) and Nigeria 75/1 (live attenuated vaccine strain). Our results showed that the highly virulent strain replicated better than the other two in PBMCs and rapidly induced cell death and a stronger inhibition of lymphocyte proliferation. However, all the strains affected lymphocyte proliferation and induced upregulation of key antiviral genes and proteins, meaning a classical antiviral response is orchestrated regardless of the virulence of the PPRV strain. On the other hand, the highly virulent strain induced stronger inflammatory responses and activated more genes related to lymphocyte migration and recruitment, and inflammatory processes. Both transcriptomic and proteomic approaches were successful in detecting viral and antiviral effectors under all conditions. The present work identified key immunological factors related to PPRV virulence in vitro.

Published
2021

55. Plant-phenotypic changes induced by parasitoid ichnoviruses enhance the performance of both unparasitized and parasitized caterpillars

Author

Cusumano, Antonino, Urbach, Serge, Legeai, Fabrice, Ravallec, Marc, Dicke, Marcel, Poelman, Erik H., Volkoff, Anne Nathalie, Cusumano, Antonino, Urbach, Serge, Legeai, Fabrice, Ravallec, Marc, Dicke, Marcel, Poelman, Erik H., and Volkoff, Anne Nathalie

Abstract

There is increasing awareness that interactions between plants and insects can be mediated by microbial symbionts. Nonetheless, evidence showing that symbionts associated with organisms beyond the second trophic level affect plant-insect interactions are restricted to a few cases belonging to parasitoid-associated bracoviruses. Insect parasitoids harbor a wide array of symbionts which, like bracoviruses, can be injected into their herbivorous hosts to manipulate their physiology and behavior. Yet, the function of these symbionts in plant-based trophic webs remains largely overlooked. Here we provide the first evidence of a parasitoid-associated symbiont belonging to the group of ichnoviruses which affects the strength of plant-insect interactions. A comparative proteomic analysis shows that, upon parasitoid injection of calyx fluid containing ichnovirus particles, the composition of salivary glands of caterpillars changes both qualitatively (presence of two viral-encoded proteins) and quantitatively (abundance of several caterpillar-resident enzymes, including elicitors such as glucose oxidase). In turn, plant phenotypic changes triggered by the altered composition of caterpillar oral secretions affect the performance of herbivores. Ichnovirus manipulation of plant responses to herbivory leads to benefits for their parasitoid partners in terms of reduced developmental time within the parasitized caterpillar. Interestingly, plant-mediated ichnoviruses-induced effects also enhance the performances of unparasitized herbivores which in natural conditions may feed alongside parasitized ones. We discuss these findings in the context of ecological costs imposed to the plant by the viral symbiont of the parasitoid. Our results provide intriguing novel findings about the role played by carnivore-associated symbionts on plant-insect-parasitoid systems and underline the importance of placing mutualistic associations in an ecological perspective., There is increasing awareness that interactions between plants and insects can be mediated by microbial symbionts. Nonetheless, evidence showing that symbionts associated with organisms beyond the second trophic level affect plant-insect interactions are restricted to a few cases belonging to parasitoid-associated bracoviruses. Insect parasitoids harbor a wide array of symbionts which, like bracoviruses, can be injected into their herbivorous hosts to manipulate their physiology and behavior. Yet, the function of these symbionts in plant-based trophic webs remains largely overlooked. Here we provide the first evidence of a parasitoid-associated symbiont belonging to the group of ichnoviruses which affects the strength of plant-insect interactions. A comparative proteomic analysis shows that, upon parasitoid injection of calyx fluid containing ichnovirus particles, the composition of salivary glands of caterpillars changes both qualitatively (presence of two viral-encoded proteins) and quantitatively (abundance of several caterpillar-resident enzymes, including elicitors such as glucose oxidase). In turn, plant phenotypic changes triggered by the altered composition of caterpillar oral secretions affect the performance of herbivores. Ichnovirus manipulation of plant responses to herbivory leads to benefits for their parasitoid partners in terms of reduced developmental time within the parasitized caterpillar. Interestingly, plant-mediated ichnoviruses-induced effects also enhance the performances of unparasitized herbivores which in natural conditions may feed alongside parasitized ones. We discuss these findings in the context of ecological costs imposed to the plant by the viral symbiont of the parasitoid. Our results provide intriguing novel findings about the role played by carnivore-associated symbionts on plant-insect-parasitoid systems and underline the importance of placing mutualistic associations in an ecological perspective.

Published
2021

58. PTPN13 induces cell junction stabilization and inhibits mammary tumor invasiveness

Author

Hamyeh, Mohamed, Bernex, Florence, Larive, Romain M., Naldi, Aurelien, Urbach, Serge, Simony-Lafontaine, Joelle, Hendriks, W.J.A.J., Freiss, Gilles, Hamyeh, Mohamed, Bernex, Florence, Larive, Romain M., Naldi, Aurelien, Urbach, Serge, Simony-Lafontaine, Joelle, Hendriks, W.J.A.J., and Freiss, Gilles

Abstract

Contains fulltext : 214494.pdf (publisher's version ) (Open Access)

Published
2020

59. PfGBP2is a novel G‐quadruplex binding protein inPlasmodium falciparum

Author

Gurung, Pratima, primary, Gomes, Ana Rita, additional, Martins, Rafael M., additional, Juranek, Stefan A., additional, Alberti, Patrizia, additional, Mbang‐Benet, Diane‐Ethna, additional, Urbach, Serge, additional, Gazanion, Elodie, additional, Guitard, Vincent, additional, Paeschke, Katrin, additional, and Lopez‐Rubio, Jose‐Juan, additional

Published
2021
Full Text
View/download PDF

65. PTPN13 induces cell junction stabilization and inhibits mammary tumor invasiveness

Author

Hamyeh, Mohamed, primary, Bernex, Florence, additional, Larive, Romain M., additional, Naldi, Aurélien, additional, Urbach, Serge, additional, Simony-Lafontaine, Joelle, additional, Puech, Carole, additional, Bakhache, William, additional, Solassol, Jérome, additional, Coopman, Peter J., additional, Hendriks, Wiljan J.A.J, additional, and Freiss, Gilles, additional

Published
2020
Full Text
View/download PDF

67. The role of parasitic larvae and their symbiotic viruses as hidden players in plant-insect interactions

Author

Cusumano, Antonino, Urbach, Serge, Volkoff, Anne Nathalie, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)

Subjects
animal structures, parasitic diseases, fungi, interaction plante insecte, food and beverages, virus, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, plant insect interaction, symbiose, human activities, symbiosis, larve
Abstract

The role of parasitic larvae and their symbiotic viruses as hidden players in plant-insect interactions. SIP/IOBC

Published
2019

68. Dual dissection of the molecular dialogue driving the bread wheat - Fusarium graminearum interaction reveals the co-regulation of candidate susceptibility factors and fungal effectors

Author

Fabre, Francis, Vignassa, Manon, Urbach, Serge, Langin, Thierry, Bonhomme, Ludovic, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), International Society of Molecular Plant Microbe Interaction., and ProdInra, Archive Ouverte

Subjects
[SDV] Life Sciences [q-bio], blé, fungal diseases, wheat, [SDV]Life Sciences [q-bio], fusarium graminearum, bread, food and beverages, pain, susceptibilité, maladie fongique
Abstract

Meeting abstract Molecular Plant-Microbe Interactions, 32(10): 203 ISSN: 0894-0282 eISSN: 1943-7706Meeting abstractMolecular Plant-Microbe Interactions, 32(10): 203ISSN: 0894-0282eISSN: 1943-7706; Fungal plant diseases are controlled by a complex molecular dialogue that involves pathogen effectors able to manipulate plant susceptibility factors at the earliest stages of the interaction. By probing the wheat-Fusarium graminearum pathosystem, we profiled the co-regulations of the fungal and plant proteins shaping the molecular responses of a 96 hour-long infection's dynamics. While no symptoms were yet detectable, fungal biomass swiftly increased along with an extremely diverse set of secreted proteins and candidate effectors supposed to target key plant organelles. Some showed to be early accumulated during the interaction or already present in spores, otherwise stored in germinating spores and detectable in an in vitro F. graminearum exudate. Wheat responses were swiftly set up and were evidenced before any visible symptom. Significant wheat protein abundance changes co-occurred along with the accumulation of putative secreted fungal proteins and predicted effectors. Regulated wheat proteins were closely connected to basal cellular processes occurring during spikelet ontogeny and particular co-regulation patterns were evidenced between chloroplast proteins and fungal proteins harboring a predicted chloroplast transit peptide. The plant and fungal coordinated responses provide a resourceful set of data and expand our understanding of the wheat-F. graminearum interaction.

Published
2019

69. SAMHD1 enhances chikungunya and zika virus replication in human skin fibroblasts

Author

Wichit, Sineewanlaya, Hamel, Rodolphe, Zanzoni, Andreas, Diop, Fode, Cribier, Alexandra, Talignani, Loïc, Diack, Abibatou, Ferraris, Pauline, Liegeois, Florian, Urbach, Serge, Ekchariyawat, Peeraya, Merits, Andres, Yssel, Hans, Benkirane, Monsef, Missé, Dorothée, Mahidol University [Bangkok], Interhuman Arbovirus Transmission (MIVEGEC-iHAT), Biologie des infections virales: Emergence, DIFfusion, Impact, Contrôle, Elimination (EDIFICE), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Theories and Approaches of Genomic Complexity (TAGC), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Virostyle (MIVEGEC-Virostyle), Perturbations, Evolution, Virulence (PEV), Zoonoses virales et MTN (MIVEGEC-VIROZ), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), University of Tartu, Centre d'Immunologie et de Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), ANR-14-CE14-0029,TIMTAMDEN,Rôle des récepteurs TIM et TAM dans l'infection des cellules cibles par le virus de la dengue(2014), ANR-16-IDEX-0006,MUSE,MUSE(2016), European Project: 734548,ZIKAlliance(2016), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), and Centre d'Immunologie et des Maladies Infectieuses (CIMI)

Subjects
viruses, Virus Replication, SILAC, Article, Cell Line, SAM Domain and HD Domain-Containing Protein 1, lcsh:Chemistry, Zika, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Viral Regulatory and Accessory Proteins, Protein Interaction Maps, lcsh:QH301-705.5, Skin, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, Zika Virus Infection, IFIT, virus diseases, Molecular Sequence Annotation, Zika Virus, Fibroblasts, SAMHD1, Up-Regulation, arbovirus, lcsh:Biology (General), lcsh:QD1-999, Proteolysis, Chikungunya Fever, Chikungunya, Chikungunya virus
Abstract

International audience; Chikungunya virus (CHIKV) and Zika virus (ZIKV) are emerging arboviruses that pose a worldwide threat to human health. Currently, neither vaccine nor antiviral treatment to control their infections is available. As the skin is a major viral entry site for arboviruses in the human host, we determined the global proteomic profile of CHIKV and ZIKV infections in human skin fibroblasts using Stable Isotope Labelling by Amino acids in Cell culture (SILAC)-based mass-spectrometry analysis. We show that the expression of the interferon-stimulated proteins MX1, IFIT1, IFIT3 and ISG15, as well as expression of defense response proteins DDX58, STAT1, OAS3, EIF2AK2 and SAMHD1 was significantly up-regulated in these cells upon infection with either virus. Exogenous expression of IFITs proteins markedly inhibited CHIKV and ZIKV replication which, accordingly, was restored following the abrogation of IFIT1 or IFIT3. Overexpression of SAMHD1 in cutaneous cells, or pretreatment of cells with the virus-like particles containing SAMHD1 restriction factor Vpx, resulted in a strong increase or inhibition, respectively, of both CHIKV and ZIKV replication. Moreover, silencing of SAMHD1 by specific SAMHD1-siRNA resulted in a marked decrease of viral RNA levels. Together, these results suggest that IFITs are involved in the restriction of replication of CHIKV and ZIKV and provide, as yet unreported, evidence for a proviral role of SAMHD1 in arbovirus infection of human skin cells.

Published
2019
Full Text
View/download PDF

72. The Syk Kinase Promotes Mammary Epithelial Integrity and Inhibits Breast Cancer Invasion by Stabilizing the E-Cadherin/Catenin Complex

Author

Kassouf, Toufic, primary, Larive, Romain, additional, Morel, Anne, additional, Urbach, Serge, additional, Bettache, Nadir, additional, Marcial Medina, Ma, additional, Mèrezègue, Fabrice, additional, Freiss, Gilles, additional, Peter, Marion, additional, Boissière-Michot, Florence, additional, Solassol, Jérôme, additional, Montcourrier, Philippe, additional, and Coopman, Peter, additional

Published
2019
Full Text
View/download PDF

75. SETDB1-dependent heterochromatin stimulates alternative lengthening of telomeres

Author

Gauchier, Mathilde, primary, Kan, Sophie, additional, Barral, Amandine, additional, Sauzet, Sandrine, additional, Agirre, Eneritz, additional, Bonnell, Erin, additional, Saksouk, Nehmé, additional, Barth, Teresa K., additional, Ide, Satoru, additional, Urbach, Serge, additional, Wellinger, Raymund J., additional, Luco, Reini F., additional, Imhof, Axel, additional, and Déjardin, Jérôme, additional

Published
2019
Full Text
View/download PDF

77. PfGBP2 is a novel G‐quadruplex binding protein in Plasmodium falciparum.

Author

Gurung, Pratima, Gomes, Ana Rita, Martins, Rafael M., Juranek, Stefan A., Alberti, Patrizia, Mbang‐Benet, Diane‐Ethna, Urbach, Serge, Gazanion, Elodie, Guitard, Vincent, Paeschke, Katrin, and Lopez‐Rubio, Jose‐Juan

Subjects
PLASMODIUM falciparum, CARRIER proteins, QUADRUPLEX nucleic acids, DNA structure, GENES, GENE knockout, TELOMERES
Abstract

Guanine‐quadruplexes (G4s) are non‐canonical DNA structures that can regulate key biological processes such as transcription, replication and telomere maintenance in several organisms including eukaryotes, prokaryotes and viruses. Recent reports have identified the presence of G4s within the AT‐rich genome of Plasmodium falciparum, the protozoan parasite causing malaria. In Plasmodium, potential G4‐forming sequences (G4FS) are enriched in the telomeric and sub‐telomeric regions of the genome where they are associated with telomere maintenance and recombination events within virulence genes. However, there is a little understanding about the biological role of G4s and G4‐binding proteins. Here, we provide the first snapshot of G4‐interactome in P. falciparum using DNA pull‐down assay followed by LC–MS/MS. Interestingly, we identified ~24 potential G4‐binding proteins (G4‐BP) that bind to a stable G4FS (AP2_G4). Furthermore, we characterised the role of G‐strand binding protein 2 (PfGBP2), a putative telomere‐binding protein in P. falciparum. We validated the interaction of PfGBP2 with G4 in vitro as well as in vivo. PfGBP2 is expressed throughout the intra‐erythrocytic developmental cycle and is essential for the parasites in the presence of G4‐stabilising ligand, pyridostatin. Gene knockout studies showed the role of PfGBP2 in the expression of var genes. Taken together, this study suggests that PfGBP2 is a bona fide G4‐binding protein, which is likely to be involved in the regulation of G4‐related functions in these malarial parasites. In addition, this study sheds light on this understudied G4 biology in P. falciparum. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

79. NEDDylation promotes nuclear protein aggregation and protects the Ubiquitin Proteasome System upon proteotoxic stress

Author

Maghames, Chantal, Lobato-Gil, Sofia, Perrin, Aurélien, Trauchessec, Hélène, Rodriguez, Manuel, Urbach, Serge, Marin, Philippe, Xirodimas, Dimitris, Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Artelia Eau & Environnement [Echirolles], Artelia Eau & Environnement [Lyon], Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Institut de Génomique Fonctionnelle (IGF), and Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Proteomics, Ribosomal Proteins, Proteasome Endopeptidase Complex, NEDD8 Protein, Science, Tumor Suppressor Proteins, Ubiquitin-Protein Ligases, Blotting, Western, Ubiquitination, Nuclear Proteins, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Real-Time Polymerase Chain Reaction, Article, HEK293 Cells, Microscopy, Fluorescence, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Cell Line, Tumor, Humans, lcsh:Q, lcsh:Science, Ubiquitins, ComputingMilieux_MISCELLANEOUS
Abstract

Spatial management of stress-induced protein aggregation is an integral part of the proteostasis network. Protein modification by the ubiquitin-like molecule NEDD8 increases upon proteotoxic stress and it is characterised by the formation of hybrid NEDD8/ubiquitin conjugates. However, the biological significance of this response is unclear. Combination of quantitative proteomics with biological analysis shows that, during proteotoxic stress, NEDDylation promotes nuclear protein aggregation, including ribosomal proteins as a major group. This correlates with protection of the nuclear Ubiquitin Proteasome System from stress-induced dysfunction. Correspondingly, we show that NEDD8 compromises ubiquitination and prevents targeting and processing of substrates by the proteasome. Moreover, we identify HUWE1 as a key E3-ligase that is specifically required for NEDDylation during proteotoxic stress. The study reveals a specific role for NEDD8 in nuclear protein aggregation upon stress and is consistent with the concept that transient aggregate formation is part of a defence mechanism against proteotoxicity., Protein NEDDylation increases upon proteotoxic stress but the function of this response remains to be elucidated. Here, the authors show that NEDDylation contributes to the cellular defence against proteotoxicity by promoting nuclear protein aggregation and protecting the ubiquitin proteasome system.

Published
2017
Full Text
View/download PDF

80. Reconstruction and signal propagation analysis of the Syk signaling network in breast cancer cells

Author

Naldi, Aurélien, Larive, Romain, Czerwinska, Urszula, Urbach, Serge, Montcourrier, Philippe, Roy, Christian, Solassol, Jérôme, Freiss, Gilles, Coopman, Peter, Radulescu, Ovidiu, Dynamique des interactions membranaires normales et pathologiques (DIMNP), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche en Cancérologie de Montpellier (IRCM - U1194 Inserm - UM), CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Service de Biopathologie [CHRU Montpellier], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), KUHN-BERAUD, Sandrine, and Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Proteomics, [SDV]Life Sciences [q-bio], Biochemistry, Tyrosine Kinases, Mathematical and Statistical Techniques, Aromatic Amino Acids, Breast Tumors, Medicine and Health Sciences, Post-Translational Modification, Phosphorylation, Amino Acids, Biology (General), Mathematical Models, Organic Compounds, hemic and immune systems, Neoplasm Proteins, Enzymes, Gene Expression Regulation, Neoplastic, [SDV] Life Sciences [q-bio], Cell Motility, Chemistry, Oncology, Physical Sciences, MCF-7 Cells, Female, Protein Interaction Networks, Network Analysis, Signal Transduction, Research Article, Computer and Information Sciences, QH301-705.5, Breast Neoplasms, Research and Analysis Methods, Models, Biological, Cell Line, Tumor, Hydroxyl Amino Acids, Breast Cancer, Humans, Syk Kinase, Computer Simulation, Gene Expression Profiling, Organic Chemistry, Chemical Compounds, Biology and Life Sciences, Proteins, Cancers and Neoplasms, Cell Biology, Signaling Networks, Random Walk, Enzymology, Tyrosine, Protein Kinases
Abstract

The ability to build in-depth cell signaling networks from vast experimental data is a key objective of computational biology. The spleen tyrosine kinase (Syk) protein, a well-characterized key player in immune cell signaling, was surprisingly first shown by our group to exhibit an onco-suppressive function in mammary epithelial cells and corroborated by many other studies, but the molecular mechanisms of this function remain largely unsolved. Based on existing proteomic data, we report here the generation of an interaction-based network of signaling pathways controlled by Syk in breast cancer cells. Pathway enrichment of the Syk targets previously identified by quantitative phospho-proteomics indicated that Syk is engaged in cell adhesion, motility, growth and death. Using the components and interactions of these pathways, we bootstrapped the reconstruction of a comprehensive network covering Syk signaling in breast cancer cells. To generate in silico hypotheses on Syk signaling propagation, we developed a method allowing to rank paths between Syk and its targets. We first annotated the network according to experimental datasets. We then combined shortest path computation with random walk processes to estimate the importance of individual interactions and selected biologically relevant pathways in the network. Molecular and cell biology experiments allowed to distinguish candidate mechanisms that underlie the impact of Syk on the regulation of cortactin and ezrin, both involved in actin-mediated cell adhesion and motility. The Syk network was further completed with the results of our biological validation experiments. The resulting Syk signaling sub-networks can be explored via an online visualization platform., Author summary The complex nature of cancer hampers traditional biological approaches to unravel its molecular mechanisms and develop targeted drug therapies. Cancer affects a number of “hallmark” cellular processes controlled by multiple signaling pathways. Our goal is to identify the pathways that negatively affect tumor development and progression. We established that the Syk protein tyrosine kinase exhibits a tumor-suppressive function in breast cancer. Large scale global biochemical analyses allowed to identify Syk targets in cancer cells, but their mechanisms and interrelationships remain unknown. Our main goal was to pinpoint a limited number of biologically realistic molecular “paths” from Syk to its effectors. We therefore developed a new methodology combining graph theoretical methods allowing to reveal the shortest “paths” between “nodes” in a graph including an approach that investigates also longer “paths”. Applied to the Syk network, this method allowed us to propose and validate new signaling axes relating Syk to major effectors of the cell adhesion and mobility that are crucial cancer hallmarks.

Published
2017
Full Text
View/download PDF

84. Recurrent domestication of viral genomes leads to different virulence strategies in ichneumonid wasps

Author

Aury, Jean Marc, Barbe, Valérie, Bezier, Annie, Drezen, Jean-Michel, Jouan, Véronique, Legeai, Fabrice, Li, L., Pichon, Apolline, Ravallec, Marc, Urbach, Serge, Volkoff, Anne-Nathalie, Institut de Génomique d'Evry (IG), Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Escola Super Agr Luiz Queiroz, Dept Entomol & Acarol, Lab Interacoes Insetos, Universidade de São Paulo (USP), Institut de Recherche sur la Biologie de l’Insecte, UMR CNRS 7261, Université Francois Rabelais [Tours], Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, China National GeneBank, Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Universidade de São Paulo = University of São Paulo (USP), Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
virulence, ichnovirus, guêpe, lutte biologique, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Published
2016

88. The cell proliferation antigen Ki-67 organises heterochromatin

Author

Sobecki, Michal, David, Alexandre, Eguren, Manuel, Birling, Marie Christine, Urbach, Serge, Hem, Sonia, Déjardin, Jérôme, Malumbres, Marcos, Jay, Philippe, Dulic, Vjekoslav, Lafontaine, Denis, Mrouj, Karim, Feil, Robert, Fisher, Daniel, Camasses, Alain, Parisis, Nikolaos, Nicolas, Emilien, Llères, David, Gerbe, François, Prieto, Susana, Krasinska, Liliana, Sobecki, Michal, David, Alexandre, Eguren, Manuel, Birling, Marie Christine, Urbach, Serge, Hem, Sonia, Déjardin, Jérôme, Malumbres, Marcos, Jay, Philippe, Dulic, Vjekoslav, Lafontaine, Denis, Mrouj, Karim, Feil, Robert, Fisher, Daniel, Camasses, Alain, Parisis, Nikolaos, Nicolas, Emilien, Llères, David, Gerbe, François, Prieto, Susana, and Krasinska, Liliana

Abstract

Antigen Ki-67 is a nuclear protein expressed in proliferating mammalian cells. It is widely used in cancer histopathology but its functions remain unclear. Here, we show that Ki-67 controls heterochromatin organisation. Altering Ki-67 expression levels did not significantly affect cell proliferation in vivo. Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. Conversely, upregulation of Ki-67 expression in differentiated tissues did not prevent cell cycle arrest. Ki-67 interactors included proteins involved in nucleolar processes and chromatin regulators. Ki-67 depletion disrupted nucleologenesis but did not inhibit pre-rRNA processing. In contrast, it altered gene expression. Ki-67 silencing also had wide-ranging effects on chromatin organisation, disrupting heterochromatin compaction and long-range genomic interactions. Trimethylation of histone H3K9 and H4K20 was relocalised within the nucleus. Finally, overexpression of human or Xenopus Ki-67 induced ectopic heterochromatin formation. Altogether, our results suggest that Ki-67 expression in proliferating cells spatially organises heterochromatin, thereby controlling gene expression., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2016

89. Origine évolutive des 'Virus-like particles' de Venturia canescens

Author

Pichon, Apolline, Urbach, Serge, Aury, Jean Marc, Bezier, Annie, Jouan, Véronique, Ravallec, Marc, Wurmser, François, Guy, Julie, Barbe, Valérie, Cousserans, Francois, Gauthier, Jérémy, Demettre, Edith, Sibut, Vonnick, Drezen, Jean-Michel, Volkoff, Anne-Nathalie, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique d'Evry (IG), Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours-Centre National de la Recherche Scientifique (CNRS), PPF Analyse des systèmes biologiques, Université Francois Rabelais [Tours], Institut de Recherche sur la Biologie de l’Insecte, UMR CNRS 7261, Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
venturia canescens, guêpe, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Published
2014

90. Acquisition of viruses as a driver in endoparasitic ichneumonid wasp evolution

Author

Pichon, Apolline, Urbach, Serge, Bezier, Annie, Aury, Jean Marc, Jouan, Véronique, Ravallec, Marc, Guy, Julie, Cousserans, Francois, FRAYSSINET, Marie, Colinet, Dominique, Barbe, Valérie, Drezen, Jean-Michel, Volkoff, Anne-Nathalie, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Faculté des Sciences et Techniques, Institut de Recherche sur la Biologie de l’Insecte, UMR CNRS 7261, Université Francois Rabelais [Tours], Institut de Génomique d'Evry (IG), Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours-Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
infection virale, virus, guêpe, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, écologie des populations, biodiversité
Published
2014

93. The cell proliferation antigen Ki-67 organises heterochromatin

Author

Sobecki, Michal, primary, Mrouj, Karim, additional, Camasses, Alain, additional, Parisis, Nikolaos, additional, Nicolas, Emilien, additional, Llères, David, additional, Gerbe, François, additional, Prieto, Susana, additional, Krasinska, Liliana, additional, David, Alexandre, additional, Eguren, Manuel, additional, Birling, Marie-Christine, additional, Urbach, Serge, additional, Hem, Sonia, additional, Déjardin, Jérôme, additional, Malumbres, Marcos, additional, Jay, Philippe, additional, Dulic, Vjekoslav, additional, Lafontaine, Denis LJ, additional, Feil, Robert, additional, and Fisher, Daniel, additional

Published
2016
Full Text
View/download PDF

94. TOM1L1 drives membrane delivery of MT1-MMP to promote ERBB2-induced breast cancer cell invasion

Author

Chevalier, Clément, primary, Collin, Guillaume, additional, Descamps, Simon, additional, Touaitahuata, Heiani, additional, Simon, Valérie, additional, Reymond, Nicolas, additional, Fernandez, Laurent, additional, Milhiet, Pierre-Emmanuel, additional, Georget, Virginie, additional, Urbach, Serge, additional, Lasorsa, Laurence, additional, Orsetti, Béatrice, additional, Boissière-Michot, Florence, additional, Lopez-Crapez, Evelyne, additional, Theillet, Charles, additional, Roche, Serge, additional, and Benistant, Christine, additional

Published
2016
Full Text
View/download PDF

95. Author response: The cell proliferation antigen Ki-67 organises heterochromatin

Author

Sobecki, Michal, primary, Mrouj, Karim, additional, Camasses, Alain, additional, Parisis, Nikolaos, additional, Nicolas, Emilien, additional, Llères, David, additional, Gerbe, François, additional, Prieto, Susana, additional, Krasinska, Liliana, additional, David, Alexandre, additional, Eguren, Manuel, additional, Birling, Marie-Christine, additional, Urbach, Serge, additional, Hem, Sonia, additional, Déjardin, Jérôme, additional, Malumbres, Marcos, additional, Jay, Philippe, additional, Dulic, Vjekoslav, additional, Lafontaine, Denis LJ, additional, Feil, Robert, additional, and Fisher, Daniel, additional

Published
2016
Full Text
View/download PDF

96. Recurrent DNA virus domestication leading to different parasite virulence strategies

Author

Pichon, Apolline, primary, Bézier, Annie, additional, Urbach, Serge, additional, Aury, Jean-Marc, additional, Jouan, Véronique, additional, Ravallec, Marc, additional, Guy, Julie, additional, Cousserans, François, additional, Thézé, Julien, additional, Gauthier, Jérémy, additional, Demettre, Edith, additional, Schmieder, Sandra, additional, Wurmser, François, additional, Sibut, Vonick, additional, Poirié, Marylène, additional, Colinet, Dominique, additional, da Silva, Corinne, additional, Couloux, Arnaud, additional, Barbe, Valérie, additional, Drezen, Jean-Michel, additional, and Volkoff, Anne-Nathalie, additional

Published
2015
Full Text
View/download PDF

98. Redundant Mechanisms to Form Silent Chromatin at Pericentromeric Regions Rely on BEND3 and DNA Methylation

Author

Saksouk, Nehmé, primary, Barth, Teresa K., additional, Ziegler-Birling, Celine, additional, Olova, Nelly, additional, Nowak, Agnieszka, additional, Rey, Elodie, additional, Simboeck, Elisabeth, additional, Mateos-Langerak, Julio, additional, Urbach, Serge, additional, Reik, Wolf, additional, Torres-Padilla, Maria-Elena, additional, Imhof, Axel, additional, and Déjardin, Jérome, additional

Published
2015
Full Text
View/download PDF

99. Quantitative phosphoproteomics reveals a cluster of tyrosine kinases that mediates SRC invasive activity in advanced colon carcinoma cells

Author

Leroy, Cédric, Fialin, Camille, Sirvent, Audrey, Simon, Valérie, Urbach, Serge, Poncet, Joël, Robert, Bruno, Jouin, Patrick, Roche, Serge, Centre de recherche en Biologie Cellulaire (CRBM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle (IGF), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1), Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), This work was supported by the CNRS, University of Montpellier 1 and 2, Association pour la Recherche contre le Cancer (ARC, n° 4025), lnstitut National du Cancer INCa and the Canceropole GSO. CL is supported by the Ligue Contre de Cancer, LCC, and AS by INCa. SR is an INSERM investigator., and Dubois, Frederic

Subjects
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Abstract

International audience; The nonreceptor tyrosine kinase Src is frequently overexpressed and/or activated in human colorectal carcinoma (CRC), and its increased activity has been associated with a poor clinical outcome. Src has been implicated in growth and invasion of these cancer cells by still not well-known mechanisms. Here, we addressed Src oncogenic signaling using quantitative phosphoproteomics. Src overexpression increased growth and invasiveness of metastatic SW620 CRC cells. Stable isotope labeling with amino acids in cell culture in combination with liquid chromatography tandem mass spectrometry allowed the identification of 136 proteins which exhibited a significant increase in and/or association with tyrosine phosphorylation upon Src expression. These mainly include signaling, cytoskeleton, and vesicular-associated proteins. Interestingly, Src also phosphorylated a cluster of tyrosine kinases, i.e., the receptors Met and EphA2, the cytoplasmic tyrosine kinase Fak, and pseudo-tyrosine kinase SgK223, which were required for its invasive activity. Similar results were obtained with metastatic Colo205 CRC cells that exhibit high endogenous Src activity. We concluded that Src uses a tyrosine kinases network to promote its invasive activity in CRC and this implicates a reverse signaling via tyrosine kinase receptors. Targeting these tyrosine kinases may be of significant therapeutic value in this cancer.

Published
2009
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results