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Yang Zhao,1,* Yi-Han Wang,2,* Wei-Chao Tu,1 Da-Wei Wang,1 Mu-Jun Lu,3,4 Yuan Shao1 1Department of Urology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, Shanghai, 201800, People’s Republic of China; 2Department of Urology, Sixth People’s Hospital South Campus Affiliated to Shanghai Jiao Tong University, Shanghai, People’s Republic of China; 3Department of Urology and Andrology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China; 4Shanghai Institute of Andrology, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Mu-Jun Lu; Yuan Shao, Email lumujun@163.com; shaoyuan10772@163.comBackground: Chronic kidney disease (CKD) is a significant worldwide health concern that leads to high mortality rates. The bioactive substance costunolide (CTD) has demonstrated several pharmacological effects and holds promise as a CKD treatment. This study aims to investigate the impact of CTD on CKD and delve into its mechanisms of action.Methods: Unilateral ureteral obstruction (UUO) methods and renal fibrosis mice models were created. Various concentrations of CTD were injected into UUO mice models to investigate the therapeutic effects of CTD on renal fibrosis of mice. Then, renal morphology, pathological changes, and the expression of genes related to fibrosis, inflammation and ferroptosis were analysed. RNA sequencing was utilized to identify the main biological processes and pathways involved in renal injury. Finally, both overexpression and inhibition of IKKβ were studied to examine their respective effects on fibrosis and inflammation in both in vitro and in vivo models.Results: CTD treatment was found to significantly alleviate fibrosis, inflammation and ferroptosis in UUO-induced renal fibrosis mice models. The results of RNA sequencing suggested that the IKKβ acted as key regulatory factor in renal injury and the expression of IKKβ was increased in vitro and in vivo renal fibrosis model. Functionally, down-regulated IKKβ expression inhibits ferroptosis, inflammatory cytokine production and collagen deposition. Conversely, IKKβ overexpression exacerbates progressive renal fibrosis. Mechanistically, CTD alleviated renal fibrosis and inflammation by inhibiting the expression of IKKβ and attenuating IKKβ/NF-κB pathway.Conclusion: This study demonstrates that CTD could mitigate renal fibrosis, ferroptosis and inflammation in CKD by modulating the IKKβ/NF-κB pathway, which indicates targeting IKKβ has an enormous potential for treating CKD. Keywords: chronic kidney disease, costunolide, renal fibrosis, IKKβ/NF-κB pathway

ObjectivesThe influence of different placements of phase change heat exchangers on the phase change process was investigated, and the simplified mode of multi-tube heat exchangers was further analyzed.MethodsBased on the enthalpy-porosity method, a calculation model for the melting and solidification process of the shell-and-tube paraffin heat exchanger was established, and the phase change process of paraffin outside the single-tube was investigated under horizontal and vertical placements. The effects of single-tube heat exchanger and multi-tube heat exchanger with straight and staggered rows on the melting and solidification process were compared, respectively.ResultsFull melting time of horizontal single-tube heat exchanger is 18% shorter than that of vertical, but the difference of full solidification time between the two is not significant. Unlike the melting process, the solidification process uses heat conduction as the main mode of heat transfer, so it is relatively slow, and the full solidification time is increased by more than 20% compared to the same period of full melting time. The time required for the melting and solidification process of multi-tube heat exchanger is slightly longer than that of the single-tube heat exchanger, but its increase in time is not more than 10%, and its melting and solidification rate curve is basically consistent with that of single-tube. Through further study, it is found that when the volume of paraffin wraps around each tube in the multi-tube heat exchanger and the wrapping method is the same, different arrangement methods of the multi-tube heat exchanger would not affect the melting and solidification process.ConclusionsCompared with the vertical placement, the horizontal placement can effectively enhance the melting rate of paraffin outside the single-tube, and the enhancement effect of the melting process is more obvious than that of the solidification process. In addition, when analyzing the performance of the melting and solidification process of the multi-tube heat exchanger, the single-tube or part of the periodic tube can be used for simplification.

In order to clarify the molecular bioinformatics characteristics and tissue expression patterns of the COBRA gene family members of Huperzia serrata, the physicochemical properties, domains, conserved motifs, cis-acting elements, and genes expression of the family members (HsCOBRAs) were analyzed by bioinformatics techniques, based on the full-length transcriptome data of the H. serrata. The results were as follows: (1) A total of 24 HsCOBRAs family members were screened in the full-length transcriptome of H. serrata, including 9 acidic proteins, 11 stabilizing proteins, 5 hydrophobic proteins, 7 proteins with transmembrane structures, and 3 proteins with signal peptides. (2) Subcellular localization was found in the cell wall, chloroplast, nucleus, and cell membrane. (3) Structural analysis revealed that HsCOBRAs had 7 domains and 6 conserved motifs, and partial members had a highly conserved CCVS structure. (4) HsCOBRAs had 45 cis-acting elements such as CAAT-box and TATA-box. (5) HsCOBRA2 had the highest expressions in leaves, spores, stems and gemma. The study results can provide theoretical basis for further research and biological function verification of HsCOBRAs.

Introduction Understanding smokers’ purchasing patterns can aid in customizing tobacco control initiatives aimed at reducing the tobacco smoking prevalence. Therefore, this study identified cigarette purchase behavior among Vietnamese male smokers and associated demographic and consumption factors. Methods We analyzed a secondary dataset of male current tobacco smokers (n=3983) who participated in the Vietnam Global Adult Tobacco Survey in 2015. We applied the latent class analysis (LCA) to identify the classes of purchase behavior among cigarette smokers (n=1241). Multinomial logistic regression was performed to identify demographics (education level, ethnicity, partnership status, and household socioeconomic status) and cigarette consumption variables (smoking years and heavy smoking status) related to purchase behavior classes. The results are reported as an adjusted relative risk ratio (ARRR). Results The LCA identified four cigarette purchase behaviors classes: Class 1 (priceinsensitive and purchased international brand: 44.4%), Class 2 (price-sensitive and purchased domestic brand: 27.6%), Class 3 (price-sensitive and purchased cigarettes in a street vendor: 18.6%), and Class 4: price-sensitive and purchased loose/carton cigarette: 9.4%). The poorer economic groups were more likely to belong to the three price-sensitive classes. Heavy smokers and those who had smoked for a longer period were more likely to belong to Class 3 (ARRR=2.33; 95% CI: 1.51–3.58 and ARRR=1.02; 95% CI: 1.001–1.05, respectively) and Class 4 (ARRR=2.94; 95% CI: 1.71–5.06 and ARRR=1.05; 95% CI: 1.02–1.08, respectively). Conclusions Varied purchasing behaviors among male cigarette smokers, influenced by divergent price sensitivities and economic backgrounds, underscore the need for comprehensive tobacco control. Future efforts should include targeted policy interventions, behavior modification, and reshaping social norms.

This study aims to estimate the influence of these factors on the ability of corporations to repay debts at commercial banks in Vietnam, especially in Ho Chi Minh City. Moreover, this study focuses on the period from 2018 to 2022. Hence, the effects of the COVID-19 pandemic were also examined in this study. In addition, this study employs a binary logistic regression method to analyze the extent of the factors' impact on the ability of corporate customers to repay debts. The results reveal that the model includes six statistically significant factors: collateral, loan term, income, firm size, leverage, net sales, and COVID-19. Furthermore, the model can be used to forecast corporations’ ability to repay debts, which could help commercial banks plan their loan strategies for corporate customers based on the significant results of the Hosmer-Lemeshow test. The study, on the other hand, only focused on some banks in the biggest city in the south of Vietnam, so further research on the area is needed, such as over Vietnam. Doi: 10.28991/ESJ-2024-08-03-016 Full Text: PDF

In this study, an efficient calculation method of matrix exponential function and vector product is proposed to reduce the calculation error. This method is based on the flexible exponential integral scheme; using B-series theory and two-color tree theory, the numerical series expression is obtained, and the exponential integral of the matrix exponential function is solved. By constructing a specific numerical integration scheme and analyzing its convergence under the theoretical framework of analytic semigroups, the convergence analysis of numerical schemes is studied and perfected. The numerical results show that the proposed method of matrix exponential function and vector product has a small error, which shows obvious advantages in reducing the calculation error.

The malfunction of endothelial progenitor cells (EPCs) due to ox-LDL is a risk contributor for arteriosclerotic disease. Meanwhile, lycopene possesses anti-inflammatory and antioxidative qualities. This investigation aimed to determine if lycopene can protect EPCs from ox-LDL-induced damage and to elucidate the underlying mechanism. The effects of lycopene on the survival, migration, and tube-forming capacity of EPCs were determined via in vitro assays. Expression of proteins related to pyroptosis and cellular proteins related to AMPK/mTOR/NLRP3 signaling was determined by western blot/flow cytometry. Our results demonstrated that lycopene treatment significantly enhanced proliferation, tube formation, and migration of EPCs stimulated by ox-LDL. Additionally, lycopene was found to suppress pyroptosis in ox-LDL-induced EPCs through the activation of AMPK, which led to the inhibition of mTOR phosphorylation and subsequent downregulation of the downstream NLRP3 inflammasome. In summary, our study suggests that lycopene mitigates ox-LDL-induced dysfunction in EPCs and inhibits pyroptosis via AMPK/mTOR/NLRP3 signaling. Our study suggests that lycopene may act as promising therapies for preventing atherosclerosis.

Abstract Recently, the use of ultrasound (US) for triggering drug release to specific tissues was explored, but its direct effects on cells have not been thoroughly understood. For this reason, this study aimed to investigate the impact of US powers and US irradiation times on fibroblast cells (NIH‐3T3). The results showed that the diverse US settings had varying effects on cell proliferation and distribution in the polystyrene culture dish. Interestingly, at 10 W, 43 kHz with changing exposed time up to 30 min either stimulated or inhibited fibroblast cell growth after 24 and 72 h of cultivation compared to the control sample in the absence of US, while longer US exposure time led to a moderate reduction in cell quantity. Moreover, higher US levels of 20 and 30 W could cause an aggregation of cells and sublethal damage to the cells. Importantly, the morphology of fibroblast was changed from stellate‐shape to round‐shape under greater US powers. Elevated US power also influenced interactions between proteins and lipids, affecting the atomic and molecular charges, leading to changes in both zeta potential and pH of the dispensed cell solution.

Lan Hong,1,* Huihui Xu,2,* Jiaqi Zheng,2 Xiujian Lin,2 Lijun Wang,2 Chengjia Zhao,3 Xiaolian Tu,4 Jingjing Zhang,5 Ke Zhao,1,6 Guohua Zhang6,7,* 1Lishui Second People’s Hospital Affiliated to Wenzhou Medical University, Lishui, 323000, People’s Republic of China; 2School of Mental Health, Wenzhou Medical University, Wenzhou, 325035, People’s Republic of China; 3School of Education, Renmin University of China, Beijing, 100872, People’s Republic of China; 4School of Renji, Wenzhou Medical University, Wenzhou, 325035, People’s Republic of China; 5School of Nursing, Wenzhou Medical University, Wenzhou, 325035, People’s Republic of China; 6Zhejiang Provincial Clinical Research Center for Mental Disorders, The Affiliated Wenzhou Kangning Hospital, Wenzhou Medical University, Wenzhou, 325035, People’s Republic of China; 7Institute Of Aging, Key Laboratory Of Alzheimer’s Disease Of Zhejiang Province, Wenzhou Medical University, Wenzhou, 325035, People’s Republic of China*These authors contributed equally to this workCorrespondence: Guohua Zhang, Zhejiang Provincial Clinical Research Center for Mental Disorders, The Affiliated Wenzhou Kangning Hospital, Wenzhou Medical University, Wenzhou, 325035, People’s Republic of China, Email zghcnu@wmu.edu.cn Ke Zhao, Lishui Second People’s Hospital Affiliated to Wenzhou Medical University, Lishui, 323000, People’s Republic of China, Email cocozk1986@163.comBackground: Bedtime procrastination (BP) has become an important factor affecting individual well-being. This study aimed to assess the stability and changes in BP and examine risk and protective factors.Methods: The study recruited 1423 respondents. Latent profile analysis was used to identify subgroups of BP and latent transition analysis to determine transition probabilities for each subgroup. Logistic regression examined associations between identified classes and related factors.Results: Three subgroups of BP were identified. In terms of stability and changes, the moderate bedtime procrastination group showed the highest stability (66%), followed by the severe bedtime procrastination group (62.4%), and the mild bedtime procrastination group had a 52% probability of switching to moderate bedtime procrastination. In terms of influencing factors, more problematic phone use (PSU) (OR: 1.08; 95% CI = 1.05– 1.12), more depression (OR: 1.17; 95% CI = 1.06– 1.29) and anxiety (OR: 1.16; 95% CI = 1.05– 1.28) are all factors that aggravate the transition from mild to moderate sleep procrastination. Similarly, PSU (OR: 1.15; 95% CI = 1.12– 1.19), anxiety (OR: 1.10; 95% CI = 1.06– 1.14), and depression (OR: 1.10; 95% CI = 1.06– 1.14) increased the risk of severe bedtime procrastination. Self-control emerged as a protective factor against BP.Conclusion: This study identified three subgroups of BP at two time points and the rule of transition for each subgroup. Our findings indicate that BP were relatively stable, with some changes over time. The results also highlight the important function that PSU, depression, anxiety, and self-control can play in preventing and intervening in BP.Keywords: bedtime procrastination, latent profile analysis, latent transition analysis, problematic smartphone use, depression, anxiety, self-control

Nanocellulose derived from plant cell wall, due to its unlimited sources, is regarded as a next-generation green material for the automotive industry because of its high tensile strength (≥3 GPa), high elasticity modulus (110–220 GPa), and low density (1.6 g/cm3). This study first introduces the structural characteristics, preparation techniques, and mechanical properties of nanocellulose. Then, three types of nanocellulose composites, including nanocellulose directly reinforced polymers, hybrid fiber-cellulose composites, and all cellulose composites (ACCs), are reviewed. The corresponding preparation techniques, material properties, reinforcement mechanisms of nanocellulose, and application limitations are discussed in detail. To overcome the insufficient mechanical properties of nanocellulose directly reinforced polymers and ACCs toward the manufacture of automobile structural components, self-assembly techniques prove to be effective to prepare macroscopic fibers by first aligning nanocellulose and then assembling them into continuous micro-size fibers. We reviewed different self-assembly techniques and multiscale modeling techniques of cellulose nanofibers (CNFs) assembled microfibers. Furthermore, we proposed a finite element or finite volume technique-based micromechanics framework to predict the homogenized responses of CNFs assembled microfibers, which serve as a fundamental layer to construct a multiscale modeling strategy toward CNFs assembled microfiber-based composite structures. The proposed multiscale modeling strategy is expected to greatly facilitate the development of CNFs assembled microfiber-based composite structures and significantly advance the application of nanocellulose in automotive structural applications.

In the contemporary urban landscape, the rail mass transit system assumes a crucial role in shaping city tourism, primarily attributable to its commendable efficiency in ensuring safety and optimizing travel times. A comprehensive understanding of this system becomes imperative for formulating strategies aimed at augmenting the tourism potential it facilitates. Consequently, this study employs rigorous spatial analysis, concentrating on tourist attractions and their accessibility levels within the Transit-Oriented Development (TOD) center and an area outside the TOD center (non-TOD). The empirical results of this investigation unveil that the distribution characteristics of a substantial proportion of tourist attractions manifest clustering tendencies (Z-score exceeding 1.65), particularly in areas synonymous with historical and recreational activities. In contrast, specific activities such as cultural and agricultural pursuits exhibit a random distribution characteristic (Z-score between −1.65 and 1.65). Notably, it becomes apparent that activities characterized by distinct distribution patterns also harbour varying potentials for accessibility. In essence, this scholarly endeavour contributes nuanced insights into the intersection of urban rail mass transit systems and tourism dynamics, shedding light on the intricate relationships between spatial distribution, activity characteristics, and accessibility levels within diverse urban contexts.

Yuan Ze,1 Hui-Ming Tu,2 Yuan-Yuan Zhao,1 Lin Zhang3,4 1Tumor Research and Therapy Center, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, People’s Republic of China; 2Department of Gastroenterology, Affiliated Hospital of Jiangnan University, Wuxi, 214122, People’s Republic of China; 3Institute of Artificial Intelligence, Hefei Comprehensive National Science Center, Hefei, 230026, People’s Republic of China; 4School of Population Medicine and Public Health, Peking Union Medical College/Chinese Academy of Medical Sciences, Beijing, 100053, People’s Republic of ChinaCorrespondence: Hui-Ming Tu, Department of Gastroenterology, Affiliated Hospital of Jiangnan University, No. 1000, Hefeng Road, Binhu District, Wuxi, 214122, People’s Republic of China, Tel +86-13861753621, Email tuhuiming1891@163.com Yuan-Yuan Zhao, Tumor Research and Therapy Center, Shandong Provincial Hospital Affiliated to Shandong First Medical University, No. 324, Jingwu Weiqi Road, Huaiyin District, Jinan, 250021, People’s Republic of China, Tel +86-13589097201, Email zhaoyuanyuan21@outlook.comPurpose: This study aimed to develop and validate a nomogram for predicting positive colonoscopy results using the data from non-invasive screening strategies.Methods: The volunteers participated in primary colorectal cancer (CRC) screenings using Asia-Pacific colorectal screening (APCS) scoring, faecal immunochemical testing (FIT) and stool deoxyribonucleic acid (sDNA) testing and underwent a colonoscopy. The positive colonoscopy results included CRC, advanced adenoma (AA), high-grade intraepithelial neoplasia (HGIN), and low-grade intraepithelial neoplasia (LGIN). The enrolled participants were randomly selected for training and validation sets in a 7:3 ratio. A model for predicting positive colonoscopy results was virtualized by the nomogram using logistic regression analysis.Results: Among the 179 enrolled participants, 125 were assigned to training set, while 54 were assigned to validation set. After multivariable logistic regression was done, APCS score, FIT result, and sDNA result were all identified as the predictors for positive colonoscopy results. A model that incorporated the above independent predictors was developed and presented as a nomogram. The C-index of the nomogram in the validation set was 0.768 (95% CI, 0.644– 0.891). The calibration curve demonstrated a good agreement between prediction and observation. The decision curve analysis (DCA) curve showed that the model achieved a net benefit across all threshold probabilities. The AUC of the prediction model for predicting positive colonoscopy results was much higher than that of the FIT + sDNA test scheme.Conclusion: The nomogram for predicting positive colonoscopy results was successfully developed based on 3 non-invasive screening tools (APCS scoring, FIT and sDNA test).Keywords: nomogram, colorectal cancer, primary screening, faecal immunochemical testing, stool deoxyribonucleic acid

Chunyan Guo,1,2,* Xiaoxiao Liu,1,* Keqing Qiu,2 Longxiang Tu,1 Dewu Liu1 1Medical Center of Burn Plastic and Wound Repair, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, People’s Republic of China; 2Department of Dermatology, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, People’s Republic of China*These authors contributed equally to this workCorrespondence: Dewu Liu, Email ndyfy00706@ncu.edu.cnPurpose: Hypertrophic scarring (HS) is commonly described as an abnormal post-traumatic tissue repair characterized by excessive hypercellularity and extracellular matrix (ECM) deposition. Mounting evidence suggests that MALAT1 is maladjusted in many fibrotic diseases, but its contribution to HS progression remains poorly understood. Hence, we sought to elucidate the fundamental role of MALAT1 in HS.Methods: The expression of MALAT1, miR-29a-3p, and Smurf2 in skin tissues and fibroblasts was assessed by RT-qPCR and Western blotting. Furthermore, lentiviruses, RNAi, or plasmids were utilized to transfect hypertrophic scar fibroblasts (HSFs) for gene overexpression or downregulation. The biological behaviors of HSFs were quantified by the CCK-8 assay, wound healing assay, transwell assay, and flow cytometry. Mechanistically, bioinformatics analysis, dual-luciferase reporter assays, and rescue experiments were performed to verify the relationship between miR-29a-3p and MALAT1 or Smurf2.Results: Our data indicate that MALAT1, Smurf2 were overexpressed while miR-29a-3p was suppressed in HS tissues and fibroblasts. Downregulation of MALAT1 may lead to decreased proliferation, migration, and invasion of fibroblasts, accompanied by enhanced apoptosis, reduced TGF-β signal transduction, and ECM accumulation in HSFs, by enhancing miR-29a-3p and suppressing Smurf2 expression. Mechanistically, MALAT1 acted as a sponge for miR-29a-3p, while miR-29a-3p directly targeted Smurf2. More importantly, rescue experiments suggested that MALAT1 downregulation induced impact on the proliferation, migration, and invasion of HSFs could be partially overturned through miR-29a-3p knockdown or Smurf2 overexpression.Conclusion: MALAT1 knockdown inhibits the proliferation, migration, invasion, and collagen deposition of HSFs via targeting the miR-29a-3p/Smurf2 axis, which may reveal a promising therapeutic exploitable vulnerability to HS. Keywords: hypertrophic scar, fibroblasts, MALAT1, miR-29a-3p, Smurf2, TGF-β signaling pathway

Abstract This study in older hospitalized patients with heart failure with reduced ejection fraction (HFrEF) aimed to examine the prevalence of beta-blocker prescription and its associated factors. A total of 190 participants were recruited from July 2019 to July 2020. The inclusion criteria included: (1) aged ≥ 60 years, (2) having a diagnosis of chronic HFrEF in the medical records, (3) hospitalized for at least 48 h. The participants had a mean age of 75.5 ± 9.1, and 46.8% were female. Of these, 55.3% were prescribed beta-blockers during admission. To explore the factors associated with beta-blocker prescription, multivariable logistic regression analysis was applied and the results were presented as odds ratios (OR) and 95% confidence intervals (CI). On multivariate logistic regression models, higher NYHA classes (OR 0.49, 95%CI 0.26–0.94), chronic obstructive pulmonary disease (OR 0.17, 95% CI 0.04–0.85), chronic kidney disease (OR 0.40, 95% CI 0.19–0.83), and heart rate under 65 (OR 0.34, 95% CI 0.12–0.98) were associated with a reduced likelihood of prescription. In this study, we found a low rate of beta-blocker prescriptions, with only around half of the participants being prescribed beta-blockers. Further studies are needed to examine the reasons for the under-prescription of beta-blockers, and to evaluate the long-term benefits of beta-blockers in elderly patients with HFrEF in this population.

Abstract Aim Acute respiratory distress syndrome or ARDS is an acute, severe form of respiratory failure characterised by poor oxygenation and bilateral pulmonary infiltrates. Advancements in signal processing and machine learning have led to promising solutions for classification, event detection and predictive models in the management of ARDS. Method In this review, we provide systematic description of different studies in the application of Machine Learning (ML) and artificial intelligence for management, prediction, and classification of ARDS. We searched the following databases: Google Scholar, PubMed, and EBSCO from 2009 to 2023. A total of 243 studies was screened, in which, 52 studies were included for review and analysis. We integrated knowledge of previous work providing the state of art and overview of explainable decision models in machine learning and have identified areas for future research. Results Gradient boosting is the most common and successful method utilised in 12 (23.1%) of the studies. Due to limitation of data size available, neural network and its variation is used by only 8 (15.4%) studies. Whilst all studies used cross validating technique or separated database for validation, only 1 study validated the model with clinician input. Explainability methods were presented in 15 (28.8%) of studies with the most common method is feature importance which used 14 times. Conclusion For databases of 5000 or fewer samples, extreme gradient boosting has the highest probability of success. A large, multi-region, multi centre database is required to reduce bias and take advantage of neural network method. A framework for validating with and explaining ML model to clinicians involved in the management of ARDS would be very helpful for development and deployment of the ML model.

Objective To examine the feasibility of implementing remote atrial fibrillation (AF) self-screening among older people supported by a remote central monitoring system.Design Process evaluation of the Mass AF randomised clinical trial (ACTRN12621000184875) with one-to-one semistructured interviews using interview guides underpinned by the Critical Realism approach and coded using the UK Medical Research Council Guidance of Process Evaluation Framework.Setting and participants Community-dwelling people aged ≥75 years from both genders (ratio 1:1) and urban/rural (ratio 2:1) in Australia.Interventions Participants were provided handheld single-lead electrocardiogram (ECG) devices and trained to self-record ECGs once daily on weekdays for at least 6 months. A remote central team notified participants and general practitioners (GPs) of AF.Primary feasibility outcomes The strengths, weaknesses, opportunities and threats (SWOT) analysis examined enablers (ie, strengths and opportunities) and barriers (ie, weaknesses and threats).Results Overall, 200 participants; 98.5% completed the 6-month programme, 96% reported being satisfied with screening and 48 were interviewed: mean age 79 years, 54% male and 71% urban. 11 GPs were interviewed: 55% female and 64% urban. Programme participants trusted the remote monitoring system that supported the screening programme and provided follow-up pathways where required. GPs saw opportunities to introduce this self-screening programme to at-risk patients and improve patients’ risk profiles. Programme participants reported that after being trained to use the device, they felt empowered to do self-screening and found it convenient. GPs saw empowerment could enhance the doctor–patient relationship. Programme participants and GPs valued screening in diagnosing AF that would otherwise be missed in usual care, but the uncertainty of effective screening duration could be a barrier.Conclusions This screening programme was feasible with the reinforcement of the underpinning enablers. Several implementation strategies were identified using SWOT analysis, including leveraging the opportunity for GPs to introduce this screening programme to at-risk patients.Trial registration number ACTRN12621000184875.

Background: Contrast-induced nephropathy is a prevalent cause of hospital-acquired renal insufficiency and increases adverse events in older patients undergoing angiography and percutaneous coronary intervention. The Mehran risk score has been widely used in Vietnam to assess contrast-induced nephropathy risk in patients before coronary angiography and percutaneous coronary intervention. Recently, there has been a shift toward the adoption of simpler risk prediction models, such as the contrast volume-to-glomerular filtration rate ratio. This study aimed to (1) determine the incidence of contrast-induced nephropathy in older patients undergoing coronary angiography and/or percutaneous coronary intervention, and (2) compare the validity of the contrast volume-to-glomerular filtration rate ratio and the Mehran score in predicting contrast-induced nephropathy. Method: This is a prospective cohort study conducted at a hospital in Vietnam from September 2019 to May 2020. Consecutive patients aged ⩾60 years who underwent coronary angiography and/or percutaneous coronary intervention were recruited. The contrast volume-to-glomerular filtration rate ratio and the Mehran score were evaluated for their predictive utility regarding contrast-induced nephropathy risk. The receiver operator characteristic was employed to calculate the area under the curve for both the contrast volume-to-glomerular filtration rate ratio and the Mehran score in predicting contrast-induced nephropathy. Results: The study included 170 participants with a mean age of 70 years and 33.1% were female. Contrast-induced nephropathy was diagnosed in 9.4% of the participants. Participants with contrast-induced nephropathy exhibited a higher prevalence of chronic kidney disease, anemia, and heart failure. There was no significant difference between the area under the curves of the contrast volume-to-glomerular filtration rate ratio (0.79, 95% CI: 0.65–0.92), and the Mehran score (0.65, 95% CI: 0.51–0.82) in predicting contrast-induced nephropathy. Conclusion: Our findings indicate that contrast-induced nephropathy was prevalent among older patients following percutaneous coronary intervention. The contrast volume-to-glomerular filtration rate ratio demonstrated a good prognostic value for predicting contrast-induced nephropathy comparable to that of the Mehran score. Further research is needed to identify optimal cutoff values for the contrast volume-to-glomerular filtration rate ratio in older patients.

Background: Renzhu Jianwei Granula (RJG) is a traditional Chinese medicine compound initially formulated to address precancerous lesions for gastric cancer. The aim of this study was to assess RJG's efficacy in treating precancerous lesions of gastric cancer through a comprehensive meta-analysis of randomized clinical trials. Methods: Two authors separately conducted an exhaustive search across three databases (PubMed, CNKI and Wanfang) without imposing any restrictions on publication year or language. Eligible studies, spanning from the inception of databases to July 18th, 2024, were included. Valid data were summarized and those with a group size of 3 or more were preserved. R software and Cochrane collaboration tools were employed for sensitivity analysis and assessing the quality of the included studies. The data from selected studies were transformed into risk ratios (RRs) and subjected to meta-analysis. This study was prospectively registered in PROSPERO. Results: Data from 9 studies encompassing 912 participants revealed that the RJG group exhibited superior clinical efficacy compared to the control group, with an RR of 0.36 (95 % confidence interval (CI): 0.25 to 0.52). RJG demonstrated enhanced efficacy over the control group in both comprehensive efficacy (RR: 0.42, 95 % CI: 0.31 to 0.55) and gastroscopy efficacy (RR: 0.56, 95 % CI: 0.46 to 0.69). Moreover, significant improvements in pathological features such as atrophy (RR: 0.58, 95 % CI: 0.45 to 0.73), dysplasia (RR: 0.41, 95 % CI: 0.27 to 0.61), and intestinal metaplasia (RR: 0.54, 95 % CI: 0.43 to 0.69) in precancerous lesions of gastric cancer were observed following RJG administration. Conclusion: This study's synthesized data provide compelling evidence of RJG's substantial therapeutic impact in ameliorating symptoms associated with precancerous lesions of gastric cancer. Trial registration number: The study protocol was registered at PROSPERO (CRD42024572606)

Despite extensive research in chemotherapy, global cancer concerns persist, exacerbated by the challenge of drug resistance, which imposes economic and medical burdens. Natural compounds, particularly secondary metabolites from medicinal plants, present promising avenues for overcoming cancer drug resistance due to their diverse structures and essential pharmacological effects. This review provides a comprehensive exploration of cancer cell resistance mechanisms and target actions for reversing resistance and highlights the in vitro and in vivo efficacy of noteworthy alkaloids, flavonoids, and other compounds, emphasizing their potential as therapeutic agents. The molecular properties supporting ligand interactions are thoroughly examined, providing a robust theoretical foundation. The review concludes by discussing methods including quantitative structure-activity relationships and molecular docking, offering insights into screening potential candidates. Current trends in clinical treatment, contributing to a holistic understanding of the multifaceted approaches to address cancer drug resistance are also outlined.

A 56-year-old man with a 5-year history of paroxysmal palpitations, which have worsened over the past year, was diagnosed with atrial fibrillation. During evaluation, transesophageal echocardiography revealed a left atrium (LA) tumoral mass attached to the atrial septal fossa ovale, with intra-tumoral blood flow and blood stream draining from the mass. Both coronary computed tomography angiography and coronary angiography demonstrated a coro-cameral fistula connection between the left circumflex artery (LCX) branch and the LA. In addition, they showed feeding arteries of the mass arising from the LCX. The patient underwent surgical resection of the LA mass and repair of the coronary artery fistula. Intraoperative exploration revealed a 1.7 cm × 1.0 cm jelly-like, brittle LA mass and confirmed a rupture of the supplying artery, leading to a coronary artery–left atrial fistula. Surgical ligation was executed to ensure complete sealing of the supplying coronary branch within the atrial septum. Histopathological examination confirmed the diagnosis of left atrial myxoma. The 6-month follow-up indicated no recurrence of the myxoma and restoration of sinus rhythm after radiofrequency ablation. In the literature, cases of a left circumflex artery branch–left atrial fistula due to rupture of the artery supplying a left atrial myxoma are rare.

Celastrol, the primary constituent of Tripterygium wilfordii, has demonstrated neuroprotective properties in rats with dementia by reducing inflammation. A high-fat diet and streptozotocin injection were utilized to establish a diabetic rat model, which was then employed to investigate the possible protective effect of celastrol against the development of diabetes-induced learning and memory deficits. Afterwards, the experimental animals received a dose of celastrol by gavage (4 mg/kg/d). An animal study showed that celastrol enhanced insulin sensitivity and glucose tolerance in diabetic rats. In the Morris water maze test, rats with diabetes performed poorly in terms of spatial learning and memory; treatment with celastrol improved these outcomes. Additionally, administration of celastrol downregulated the expression of inflammatory-related proteins (NF-κB, IKKα, TNF-α, IL-1β, and IL-6) and greatly reduced the generation of Aβ in the diabetic hippocampus tissue. Moreover, the insulin signaling pathway-related proteins PI3K, AKT, and GSK-3β were significantly upregulated in diabetic rats after celastrol was administered. Also, celastrol prevented damage to the brain structures and increased the synthesis of synaptic proteins like PSD-95 and SYT1. In conclusion, celastrol exerts a neuroprotective effect by modulating the insulin signaling system and reducing inflammatory responses, which helps to ameliorate the cognitive impairment associated with diabetes.

This study develops a model to raise public awareness about the consequences of burning rice straw after harvest, including environmental pollution, soil degradation, and increased CO 2 emissions that contribute to the greenhouse effect. The distinctive feature of the research is the introduction of a post-harvest rice straw treatment process using microbial products capable of secreting cellulase enzymes, which can break down the cellulose in the straw. This process shortens the decomposition time and produces natural organic fertilizer, thus reducing cultivation costs by 60% and increasing crop yields by 20%. The experimental model was carried out in Cam My district, Dong Nai province, Vietnam, including 4 models: no microbial products; using Bio Decomposer; using NTT-01; and using NTT-02. Each experimental field had an area of 650 m². The results showed a significant reduction in straw decomposition time after 14 days of use of the products, with a decomposition rate of up to 80%, nearly twice as fast as without the products. This helps save time, produce natural organic fertilizers, reduce care costs, and increase rice yields, resulting in more income for local residents. These findings demonstrate the effectiveness of microbial treatments in sustainable agriculture and their potential for a broader application in the management of agricultural waste.

Objective: This investigation explored the association between postoperative/preoperative platelet ratio (PPR) and the incidence of unfavorable outcomes within 90 days in individuals with aneurysmal subarachnoid hemorrhage (aSAH). Methods: This investigation, utilizing data from 2015 to 2022, concentrated on patients diagnosed with aSAH, categorizing them into four groups based on PPR quartiles. The association between PPR levels and clinical outcomes—comprising in-hospital complications, mortality, and modified Rankin Scale (mRS) scores at discharge and 90 days after that—was evaluated through logistic regression analyses. To explore potential non-linear associations between PPR levels and outcomes, restricted cubic spline (RCS) regression was applied. Further, mediation analysis was performed to elucidate the role of in-hospital complications in modulating the impact of PPR levels on 90-day outcomes. Results: This study analyzed data from 948 patients. Upon adjustment for confounding variables, it was observed that patients in the higher quartiles showed reduced incidences of anemia, hypoproteinemia, and pneumonia, alongside a decreased frequency of unfavorable outcomes within a 90-day follow-up period. The RCS analysis indicated a linear association of PPR with pneumonia, hypoproteinemia, and adverse 90-day outcomes (p for nonlinear = 0.61, 0.52, and 0.96, respectively). Moreover, the association of PPR with anemia was found to be nonlinear (p for nonlinear = 0.01). Mediation analysis further indicated that anemia and pneumonia significantly influenced the association between PPR and unfavorable outcomes at 90 days, accounting for 15.49 % and 27.61 % of the effect, respectively. Conclusions: This study establishes a significant correlation between decreased PPR levels and 90-day adverse outcomes following aSAH, potentially relating to pneumonia and anemia.

Objectives: Evaluating the physical health, mental health, and Quality of life (QoL) in relationship to body constitution (BC) types in students of the Faculty of Traditional Medicine (FTM) in the University of Medicine and Pharmacy at Ho Chi Minh City (UMP). Methods: A cross-sectional descriptive study on 717 students of FTM through face-to-face interviews used three questionnaires to investigate the following sections: general demographic information questionnaire; physical health (PCS), mental health (MCS), and QoL (Vietnamese version of SF-36 questionnaire); BC types (Vietnamese version of the CCMQ questionnaire). We used multiple linear regression analyses to assess the associations between BC types and each of the QoL domains’ scores. The dependent variables are the scores of eight QoL domains and integration scores in PCS and MCS. Results: Most students had Unbalanced BC types (87.03 %). Qi-depression, Qi-deficiency, and Blood-stasis had the three highest percentages (48.1 %, 33.2 %, 26.9 %). There were relationships between BC types and students’ physical health, mental health, and QoL. The SF-36 resulted in “high” scores in most of the Neutral-type students, while the majority of students with Unbalanced BC types had “poor” scores in both physical health, mental health, and QoL (p

BackgroundChronic inflammation is closely linked to Chronic Obstructive Pulmonary Disease (COPD); however, the impact of the Dietaryq Inflammatory Index (DII) on mortality among COPD patients remains uncertain.ObjectiveTo assess the correlation between the DII and all-cause mortality in COPD patients using data from the National Health and Nutrition Examination Survey (NHANES).MethodsWe conducted a retrospective cohort study on 1,820 COPD patients from the NHANES dataset (1999-2018). The influence of DII on mortality was evaluated using multivariate Cox regression, smoothing spline fitting, and threshold effect analysis. Additionally, Kaplan-Meier survival analysis was performed to compare survival curves among different DII groups. Subgroup analyses and E-values identified sensitive cohorts and assessed unmeasured confounding.ResultsOver an average follow-up of 91 months, multivariate Cox regression models revealed a significant positive correlation between DII scores and mortality risk, with each unit increase in DII associated with a 10% higher risk of death (HR: 1.10, 95% CI: 1.03-1.16; P = 0.002). Among the DII tertiles, individuals in the second tertile (T2: 1.23-2.94) experienced a 67% increase in mortality risk compared to those in the lowest tertile (T1: -5.28-1.23) (HR: 1.67, 95% CI: 1.26-2.21; p < 0.001). The third tertile (T3) did not show a statistically significant increase in mortality risk (HR: 1.30, 95% CI: 0.98-1.72; p=0.074). A restricted cubic spline analysis indicated a significant nonlinear association between DII and all-cause mortality (p = 0.021). Threshold effect analysis further revealed that below a DII of 2.19, there was a significant increase in all-cause mortality risk (HR = 1.19, 95% CI: 1.07-1.33; p = 0.002), while at or above this threshold, the risk increase was not statistically significant (HR=0.89, 95% CI: 0.68-1.15; p = 0.380). Kaplan-Meier analysis revealed significant differences in survival curves among DII tertiles (p < 0.001), with the lowest DII tertile showing the highest survival probability. Both subgroup and sensitivity analyses confirmed the robustness of these findings.ConclusionDII is positively correlated with mortality risk in COPD patients, showing nonlinear characteristics and threshold effects, underscoring its prognostic value.

Background Digital Health Literacy (DHL) is crucial in navigating digital health environments, yet few studies focus on older adults. Objective Explore the associations of digital health information and resource utilization, IT-related social support, and barriers/enhancers to digital health service usage with DHL among older adults. Methods A cross-sectional study was conducted from January 2022 to April 2023, involving 417 individuals over the age of 60 who were interviewed using an instrument for collecting data on DHL, social support, barriers/enhancers influencing use of digital health resources and personal/demographic data. Multi-regression models were used to examine the associations. Results Higher DHL scores were associated with daily use of digital interaction with healthcare ( B = 0.28; 95% confidence interval [CI] = 0.07, 0.49; p = .01), daily use of other digital health resources ( B = 0.22; 95% CI = 0.05, 0.40; p = .01), ease in finding assistance for online navigation ( B = 0.27; 95% CI = 0.08, 0.45; p = .01), self-perceived digital proficiency (“usually very good at surfing the internet,” B = 0.35; 95% CI = 0.18, 0.52; p = .01), assistance from relatives/others in internet browsing ( B = 0.20; 95% CI = 0.02, 0.37; p = .02), and having access to a computer, tablet, or smartphone ( B = 0.29; 95% CI = 0.11, 0.47, p = .01). Conversely, barriers like “no access to a computer, tablet, or phone at all times” ( B = −0.19; 95% CI = −0.34, −0.04; p = .01), “difficulty understanding online content” ( B = −0.22; 95% CI = −0.36, −0.07; p = .01), and “believing to be too old for online services” ( B = −0.18; 95% CI = −0.32, −0.03; p = .02) were associated with lower DHL scores. Conclusions Engagement with digital health platforms, including making online appointments and accessing personal health records, is associated with higher DHL levels. Support from relatives or others, a modifiable attribute, is also associated with elevated DHL among older adults.

Background: Adenylyl cyclase (AC) isoforms played a key role in the multiple cancer pathology, However, the expression, prognostic value and function of ADCY5 in Glioblastoma (GBM) have not been reported yet. This research intends to discover the expression, epigenetic alteration and biological function of ADCY5 in GBM and its value on patients' prognosis. Methods: ① Transcriptional level, epigenetic alteration, prognostic value and molecular network of ADCY5 were analyzed by using of public online datasets. ② The mRNA expression profile of ADCY5 was explored by using GEPIA database and protein expression levels were detected by HPA Database. ③ The prognostic value of ADCY5 was determined by Kaplan-Meier Plotter, GEPIA and CGGA database. ④ The epigenetic characteristics of ADCY5 were determined by DiseaseMeth database. ⑤ Identification of genes co-expressed with ADCY5 and potential mechanism analyses were performed by using DAVID cBioPorta and STRING. ⑥ Reverse transcription-polymerase chain reaction (RT-PCR), cell counting kit-8 (CCK-8), colony formation, wound-healing scratch and transwell assay were applied to detect relative mRNA expression and biological function of ADCY5 in GMB cells. Results: ADCY5 mRNA and protein were downregulated in GBM compared with normal tissues. Analysis of the genetics and epigenetics of ADCY5 suggested that its expression was negatively correlated with DNA methylation. High expression of ADCY5 was significantly associated with age, grade, IDH mutation, 1p19q_codeletion, radiotherapy and chemotherapy and acted as an independent prognostic factor in GBM. ADCY5 mRNA also down-expressed in GBM cell lines and re-expressed of ADCY5 could inhibit cell proliferation, viability, migration/invasion and epithelial-mesenchymal transition (EMT) in vitro. In the analysis of genes co-expressed with ADCY5, we found that cAMP/AKT pathway, cGMP-PKG pathway, Wnts pathway were dissimilarly enriched. Conclusion: Our study indicated that ADCY5 could act as an epigenetic biomarker in GBM, as well as a prognosis target in patients with GBM.

IntroductionThis study aimed to evaluate the status, resource use, and challenges of the capture-based hatchery of black tiger shrimp (Penaeus monodon) (BTS).MethodsData was obtained through the investigation of 15 broodstock fishing boats and 60 capture-based BTS broodstock hatcheries. Resource use index (RUI) of the capture-based BTS hatchery, calculated from 1,000 female brooders to produce 15-day-old post-larvae (PL15) (production efficiency of PL15, or PE-PL15), was analysed.ResultsThe results indicated that the fishing boats captured an average of 5,900 brooders.boat-1.year-1, 20,856 kg of by catch.boat-1.year-1, and the PE-PL15 production of 937 million for 1,000 female brooders. In the hatchery stage, the use of larger body weight female brooders had benefits in terms of higher survival rate and better efficiency of labour use, Artemia cyst, and probiotic products, but it reduced the efficiency use of chemicals, pellets, and total tank volume of the hatchery.DiscussionThere is increasing use of domesticated brooder sources, but wild-caught brooders still play an important role in producing low-cost PL15 for traditional shrimp farming systems, accounting for most farmed BTS production. Therefore, the certification of good practices should be implemented to capture-based BTS hatcheries for the reasonable use of this brooder resource to sustain BTS farming in Vietnam.

Bacillus velezensis RB.IBE29 harbors two chitinases belonging to the glycoside hydrolase family 18 and exhibiting a novel domain structure. The roles of these chitinases in crop production have been reported; nevertheless, their contribution to controlling human pathogens is unknown. In this initial work, the chitinases A (BvChiA) and B (BvChiB) of strain RB.IBE29 were produced in recombinant Escherichia coli BL21-CodonPlus (DE3)-RIPL cells and subsequently purified using HisTrap FF column. The purified BvChiA and BvChiB exhibited the highest chitinase and binding activities against colloidal chitin. Combining both chitinases for the hydrolysis of powdered chitin increased the reducing sugar content by 88.7 %. Moreover, the purified chitinases remarkably suppressed the germination of Candida albicans VTCC 20568 (=JCM 2070) cells. These results indicated that the novel domain-structure-containing chitinases of strain RB.IBE29 have great potential and can be further developed as a novel therapeutic agent against human pathogenic C. albicans.

Light–matter interaction operating in the strong coupling regime offers wide prospects of applications going from nano-photonics to quantum communications. The most practical implementations are to embed the active matter into Fabry–Perot microcavities or photonic crystals. In this work we focus on the strong coupling of two-dimensional (2D) perovskite in 1D grating waveguide. We use rigorous coupled wave analysis to simulate electromagnetic wave confinement in the 1D waveguide. Various sets of waveguide geometrical parameters are examined to achieve the strong coupling regime for three different configurations of the active layer in the waveguide. To extract quantitatively the relevant physical parameters, such as strength of light–matter interaction, we develop a Hamiltonian formalism to reproduce results obtained by simulation. It is shown that the strongest interaction is to have the active layer inserted in the main slab of the waveguide. It is, however, still weaker by about 20% as compared to the use of Fabry–Perot microcavities.

This paper introduces a 1-D finite difference model for analyzing the temperature distribution throughout the hardening phase in concrete structures. The model accounts for the degree of hydration-dependent internal heat rate and the solar irradiance on the receiving surface of a concrete structure, allowing for more accurate predictions of early-age behavior of concrete. The research involves the development and validation of the 1-D model using temperature measurements from an on-site concrete footing. Mesh sensitivity studies were conducted to optimize grid size for thermal analysis. The paper also explores the determination of suitable 2-D width-to-height ratios for employing the 1-D model, providing practical guidelines for its application. Major findings indicate that the 1-D model can accurately predict temperature evolution in early-age concrete and offers a cost-effective alternative to more complex 2-D, 3-D finite difference, and finite element models. For preliminary analysis and quick assessments, the 1-D model is advantageous due to its computational speed, and it holds significant potential for concrete temperature control and construction optimization.

Hot spring (HS) waters manifest diverse positive effects on the skin due to their unique chemical compositions. Sodium hyaluronate acid (HA) comprises N-acetylglucosamine and D-glucuronic acid, and distinguishes itself with superior qualities in skin regeneration, providing moisturizing and anti-aging benefits. The combination of HA with HS water is widely applied across ophthalmology, pneumology, nutrition, and cosmetics. This study delved into the application of HA in cosmetology, with a focus on its interaction with HS water and its effects on moisture retention and promoting wound healing. In particular, with the alkaline pH levels of the Yumoto HS, HA molecules may undergo dissociation to be ionized resulting in a negatively charged polymer and interacting with positively charged ions in the HS water through electrostatic interactions. The shifted peaks in the FTIR result and zeta potential shifts to a less negative region in the case of HA-HS compared to HA-DI indicate an ionic interaction between HS water and HA. Moisture tests confirmed the sustained hydration when HA is dissolved in HS water, underscoring its potential to improve skin hydration at certain concentrations, specifically at 0.5% and 1%. Additionally, MTT assay results demonstrated that HS water stimulates the growth of fibroblast cells compared to distilled water, implying its potential beneficial effect in wound healing. These findings suggested the multifaceted benefits of HAHS in skincare, highlighting its role in enhancing skin hydration and potentially accelerating wound healing processes, thus presenting avenues for the development of advanced cosmeceutical formulations.

The purpose of this study was to investigate the perceptions of EFL pre-service teachers regarding the integration of ICT in EFL classrooms during teaching practicum. The study involved 40 EFL pre-service teachers from a university in Vietnam and employed a mixed-method design to achieve its objectives. A questionnaire based on the Technology Acceptance Model was developed to gather quantitative data, and semi-structured interviews were conducted to obtain in-depth information. The results obtained from both quantitative and qualitative data indicated that the EFL pre-service teachers generally had positive perceptions of using ICT in EFL classrooms during their teaching practicum. The study also revealed several suggestions for improving ICT integration in the teaching and learning process.

Objective·To analyze the epidemiological characteristics of risk factors for cardiovascular diseases and malignant tumors based on the Shanghai community elderly cohort.Methods·The study subjects were selected from the Shanghai community elderly cohort established from February to August 2019, with a total of 17 948 people. The study subjects were divided into 4 groups according to self-reported presence or absence of tumors and/or cardiovascular diseases during the baseline survey: tumor-free and non-cardiovascular disease group, single cardiovascular disease group, single tumor group and tumor cardiovascular disease co-occurrence group. The differences among the four groups of subjects were collected and compared in terms of demographic characteristics and physiological indicators, daily living habits (smoking, drinking tea, drinking coffee, drinking carbonated drink, drinking alcohol, sedentary time, physical activity level and sleep quality), past medical history, psychological status (depression and anxiety) and dietary compliance.Results·Among the study subjects, 60.1% of tumor patients were complicated with cardiovascular diseases. The differences among the four groups of subjects in age, gender, educational level, pre-retirement occupation, waist circumference, hip circumference and body mass index were statistically significant (all P

Di Zhang,1,&ast; Yu Guan,1,&ast; Xiuxiu Zhou,1 Mingzi Zhang,2 Yu Pu,1 Pengchen Gu,2 Yi Xia,1 Yang Lu,2 Jia Chen,2 Wenting Tu,1 Kunyao Huang,2 Jixin Hou,2 Hua Yang,2 Chicheng Fu,2 Qu Fang,2 Chuan He,2 Shiyuan Liu,1 Li Fan1 1Department of Radiology, Second Affiliated Hospital, Naval Medical University, Shanghai, People’s Republic of China; 2Scientific Research Department, Shanghai Aitrox Technology Corporation Limited, Shanghai, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Li Fan; Shiyuan Liu, Department of Radiology, Second Affiliated Hospital, Naval Medical University, 415 Fengyang Road, Shanghai, 200003, People’s Republic of China, Tel +13564684699 ; +13761304518, Fax +86 21 63587668, Email czyxfl@smmu.edu.cn; radiology_cz@163.comPurpose: To develop a novel method for calculating small airway resistance using computational fluid dynamics (CFD) based on CT data and evaluate its value to identify COPD.Patients and Methods: 24 subjects who underwent chest CT scans and pulmonary function tests between August 2020 and December 2020 were enrolled retrospectively. Subjects were divided into three groups: normal (10), high-risk (6), and COPD (8). The airway from the trachea down to the sixth generation of bronchioles was reconstructed by a 3D slicer. The small airway resistance (RSA) and RSA as a percentage of total airway resistance (RSA%) were calculated by CFD combined with airway resistance and FEV1 measured by pulmonary function test. A correlation analysis was conducted between RSA and pulmonary function parameters, including FEV1/FVC, FEV1% predicted, MEF50% predicted, MEF75% predicted and MMEF75/25% predicted.Results: The RSA and RSA% were significantly different among the three groups (p< 0.05) and related to FEV1/FVC (r = − 0.70, p < 0.001; r = − 0.67, p < 0.001), FEV1% predicted (r = − 0.60, p = 0.002; r = − 0.57, p = 0.004), MEF50% predicted (r = − 0.64, p = 0.001; r = − 0.64, p = 0.001), MEF75% predicted (r = − 0.71, p < 0.001; r = − 0.60, p = 0.002) and MMEF 75/25% predicted (r = − 0.64, p = 0.001; r = − 0.64, p = 0.001).Conclusion: Airway CFD is a valuable method for estimating the small airway resistance, where the derived RSA will aid in the early diagnosis of COPD.Keywords: COPD, small airway disease, CT, fluid dynamics

In order to solve the problem of lacking velocity feedback information in the velocity closed-loop control system for linear induction motors (LIM) after cancelling the velocity sensor, a velocity observer based on the extended Kalman filtering algorithm has been implemented, considering the end edge effect of LIM. Firstly, based on the mathematical model of three-phase linear induction motor considering edge effect, an extended Kalman filter observer with appropriate gain and covariance update matrix is derived. Based on the vector control system of LIM, the speed parameters identified by the observer are fed back to the speed closed-loop system. Then, the vector control system model of linear induction motor with speed observer is built in Simulink, and the identification speed of observer is compared with the actual speed of motor. Finally, the results show that the closed-loop control using the identification speed can ensure the stable operation of the system. Under three kinds of loads, the error between the predicted speed and the actual speed of the observer is form 0.51% to 2.34%. The various dynamic performance of the system reveals that the LIM vector control system based on the extended Kalman filter observer increases prediction velocity error and decreases thrust error with increasing load. However, the magnetic flux amplitude error slightly increases. Therefore, considering the end-edge effect, the observer based on the extended Kalman filter can replace the velocity sensor to achieve three-phase LIM control under both unloaded and loaded conditions.

Abstract 5q-spinal muscular atrophy (SMA) is a neuromuscular disorder (NMD) that has become one of the first 5% treatable rare diseases. The efficacy of new SMA therapies is creating a dynamic SMA patient landscape, where disease progression and scoliosis development play a central role, however, remain difficult to anticipate. New approaches to anticipate disease progression and associated sequelae will be needed to continuously provide these patients the best standard of care. Here we developed an interpretable machine learning (ML) model that can function as an assistive tool in the anticipation of SMA-associated scoliosis based on disease progression markers. We collected longitudinal data from 86 genetically confirmed SMA patients. We selected six features routinely assessed over time to train a random forest classifier. The model achieved a mean accuracy of 0.77 (SD 0.2) and an average ROC AUC of 0.85 (SD 0.17). For class 1 ‘scoliosis’ the average precision was 0.84 (SD 0.11), recall 0.89 (SD 0.22), F1-score of 0.85 (SD 0.17), respectively. Our trained model could predict scoliosis using selected disease progression markers and was consistent with the radiological measurements. During post validation, the model could predict scoliosis in patients who were unseen during training. We also demonstrate that rare disease data sets can be wrangled to build predictive ML models. Interpretable ML models can function as assistive tools in a changing disease landscape and have the potential to democratize expertise that is otherwise clustered at specialized centers.

Yanchen Chen,1,2 Yuqin Tu,2,3 Guiling Yan,2,3 Xinyao Ji,2,3 Shu Chen,2,3 Changchun Niu,2 Pu Liao1,2 1Department of Laboratory Medicine, North Sichuan Medical College, Nanchong, People’s Republic of China; 2Department of Clinical Laboratory, Chongqing General Hospital, Chongqing, People’s Republic of China; 3Chongqing Medical University, Chongqing, People’s Republic of ChinaCorrespondence: Pu Liao, Chongqing General Hospital, 118 Xingguang Avenue, Yubei District, Chongqing, People’s Republic of China, Tel +86 13628358003, Fax +23-63519127, Email liaopu@sina.comPurpose: There is growing evidence that the immune system plays an important role in the progression of Parkinson’s disease, the second most common neurodegenerative disorder. This study aims to address the comprehensive understanding of the immunopathogenesis of Parkinson’s disease and explore new inflammatory biomarkers.Patients and Methods: In this study, Immune-related differential expressed genes (DEIRGs) were obtained from GEO database and Immport database. The hub gene was screened in DEIRGs using LASSO regression and random forest algorithm, and the mRNA expression of the identified hub gene was validated using clinical blood samples.Results: We obtained a total of 157 DEIRGs that played an important role in the immune response. The results of immune cell infiltration analysis showed that the degree of memory B cells infiltration was higher in PD patients, while the degree of Monocytes, resting mast cells and M0 macrophages infiltration was lower (p< 0.05). A total of 8 hub genes were screened by machine learning methods, and RT-PCR results showed that the expression level of CBL gene in PD was significantly increased (p< 0.05).Conclusion: Our findings suggest that CBL is a new potential diagnostic biomarker for PD and that abnormal immune cell infiltration may influence PD development.Keywords: Parkinson’s disease, immune cell infiltration, bioinformatics, immport database, gene expression omnibus database

Recent metaheuristic approaches are extensively and intensively being implemented to the interpretation of gravity anomalies due to their superior advantages. We emphasize the application of Hunger Games Search (HGS), a newly established metaheuristic inspired by hunger-driven instincts and behavioral choices of animals, to elucidate gravity data for geothermal energy exploration and volcanic activity study. After recognizing the modal features of the objective function tailored and tuning the algorithm control parameters involved, HGS has been trial-tested on simulated data sets of different scenarios and finally experienced in two field cases from India and Japan. Notably, a second moving average strategy has been successfully integrated into the objective function to eradicate the regional component from observed responses. Post-inversion uncertainty appraisal tests have been further implemented to comprehend the reliability of solutions obtained. The solutions retrieved by HGS have been unbiasedly compared in terms of convergence rate, accuracy, stability, and robustness with the solutions of the commonly used particle swarm optimization algorithm. Based on the results accessed, the theoretical and field cases presented could be recuperated more precisely, stably, robustly, and coherently with the available geophysical, geological, and borehole verification, as HGS is able to better explore the model space without compromising its capability to efficiently approach the global minimum. This novel global optimization method can thus be considered as a promising tool in geothermal energy investigations and the study of volcanic activities.

Context: Adverse drug interactions increase the risk of hospitalization, treatment costs, unfavorable treatment outcomes, and prolonged hospital stays. However, there are a limited number of studies conducted in city-affiliated healthcare settings in Vietnam. Aims: To identify drug interactions in outpatient treatment prescriptions at a city-based healthcare center in the southern region of Vietnam. Methods: A cross-sectional descriptive study was conducted on outpatient treatment prescriptions stored in the outpatient department of a city-affiliated healthcare center from January to June, 2023. The study used two databases for drug interaction checking: the Drug Interactions Checker and Medscape Interactions Checker. Univariate and multivariate regression analyses were used to identify predictors of clinically significant drug interactions. Results: Among 1203 surveyed prescriptions, 36.7% contained at least one clinically-significant drug interaction, with 3% of prescriptions having severe interactions. The highest frequency of drug interactions involved amlodipine and metformin (9.9%). Patients with five or more prescribed medications (OR = 3.305; 95% CI: 2.320-4.708), hypertension (OR = 7.142; 95% CI: 4.658-10.950), dyslipidemia (OR = 2.242; 95% CI: 1.665-3.020), and non-insulin-dependent diabetes (OR = 2.447; 95% CI: 1.817-3.295) had a higher likelihood of experiencing clinically significant drug interactions. Conclusions: This study revealed that over one-third of outpatient prescriptions were associated with clinically-significant drug interactions. This study highlights the importance of clinical pharmacists screening prescriptions to detect and reduce interactions, as well as the application of computerized alert systems with intelligent screening software to minimize drug interactions.

[Objective] Ammonia is acknowledged as an efficient hydrogen storage medium, having the potential of being a substitute for fossil fuels in achieving the vision of a “carbon-free future”. Due to its stable liquid-state attributes in storage and transport, establishing a transmission system integrating green ammonia and product oil using in-service product oil pipelines is of important significance for fostering a green ammonia industry chain. [Methods] This paper began with a comprehensive introduction to background information,including the production capacity distribution of green ammonia in China, the pros and cons of different transportation modes, and the potentiality of integrating liquid ammonia transmission into in-service product oil pipelines. Then the adaptability of the system in integrating liquid ammonia transmission was analyzed from six dimensions: properties of transmission mediums, long-distance pipeline structure, pipes and equipment, pipeline transmission technology, hydraulic calculation method, and safety considerations related to operational pressure and fow rates. Considering the coupling characteristics of green ammonia and product oil during transportation, a conceptual plan was outlined for establishing green ammonia and product oil integrated transmission systems based on the supply and demand patterns of “westeast ammonia transmission” and “north-south ammonia transmission”. [Results] As the production capacity of green ammonia continuously expands, pipelines have emerged as the preferred mode for long-distance transportation. Moreover, given the underutilized transmission capacity of existing product oil pipelines, notable potential exists in integrating liquid ammonia transmission into these pipelines to enhance economic benefits. Theoretically, current product oil pipelines bear striking resemblance to existing long-distance liquid ammonia pipeline systems, so they are capable of meeting specific requirements for liquid ammonia transport when offered with technological or equipment upgrades. Therefore, under the premise of implementing rational pressure and flow rate control strategies, the system integrating liquid ammonia transmission into operational product oil pipelines exhibits robust adaptability, necessitating further experimental validation of their feasibility. This paper envisioned the potential of these integrated systems across four key aspects: planning for green ammonia and product oil integrated transmission systems, optimization through collaboration of multiple transportation modes, defining operational boundaries for liquid ammonia pipeline transmission, and optimization of pipeline transmission batches. [Conclusion] The study outcomes can serve as a valuable reference for promoting the healthy development of the green ammonia industry and enhancing the safety and economic efficiency of green ammonia and product oil integrated transmission systems.

Uric acid (UA), the final product of human purine metabolism, can cause hyperuricemia (HUA) when excessively accumulated. HUA is closely linked to chronic kidney diseases (CKD) and is considered an independent risk factor. Hyperuricemic nephropathy, a form of CKD induced by HUA, has seen significant advances in understanding through research into the pathogenic roles of uric acid and the development of HUA animal models. Although progress has been made in understanding the pathophysiological mechanisms by which UA induces CKD, much remains to be learned about its pathological molecular mechanisms. New approaches in animal modeling or the selection of model animals may potentially lead to significant breakthroughs in research on hyperuricemia as well as related CKD. This paper reviews the research progress on the molecular mechanisms of hyperuricemic nephropathy, focusing on oxidative stress, inflammation, autophagy, fibrosis, and gut microbiota. Oxidative stress is induced by uric acid intracellularly through xanthine oxidase, NADPH oxidases, and mitochondria, leading to cellular damage. In terms of inflammation, uric acid crystals can activate the NLRP3 inflammasome, triggering an inflammatory cascade. The role of free uric acid as a pro-inflammatory agent, however, remains controversial. Depending on the study conducted, autophagy has been found to either alleviate or exacerbate inflammation induced by uric acid. Fibrosis, particularly through epithelial-mesenchymal transition (EMT), is a major mechanism by which uric acid causes glomerulosclerosis and tubulointerstitial fibrosis. Extensive research has explored various signaling pathways involved in uric acid-induced EMT. Beneficial gut microbiota protect the kidneys by synthesizing short-chain fatty acids, reducing urea’s enterohepatic circulation, and decreasing uric acid production. This paper aims to enhance understanding of the complex relationships between HUA and CKD, serving as a reference for further research and new drug development.

Objective To investigate the high risk factors of endometrial polyps (EPs) in infertile patients and its impact on the pregnancy outcome after embryo transfer. Methods A case-control trail was conducted on the infertility patients who undergoing embryo transfer in our hospital for the first time after hysteroscopy from January 2016 to December 2022.Their clinical data were collected and retrospectively analyzed.Univariate and stepwise logistic regression analyses were used to identify the risk factors for EPs, and the impact of polyps on the pregnancy outcomes of assisted reproductive pregnancy was analyzed with propensity score matching (PSM) at a 1:2 ratio. Results A total of 388 patients diagnosed with EPs and undergoing hysteroscopic endometrial polypectomy were assigned into the EPs group, and 2 163 non-polyp patients were into the non-EPs group.Univariate analysis showed statistical differences were observed in age[31(29, 34)vs 31(28, 33), P=0.002], history of pelvic inflammatory disease (42.78%vs 64.17%, P=0.000), age at menarche[14(12, 14)vs 13(12, 14) years old, P=0.000], number of pregnancies[0(0, 1)vs 1(0, 2), P=0.000], primary infertility (60.30%vs 50.20%, P=0.000), duration of infertility[4(2.1, 6.0)vs 4(2.0, 6.0) years, P=0.002], concomitant endometriosis (9.53%vs 6.52%, P=0.032), concomitant uterine fibroids (11.85%vs 6.93%, P=0.001), and basal estrogen level[38.12(27.00, 59.00)vs 36.00(25.00, 53.00)μg/L, P=0.016]between the 2 groups.Logistic stepwise regression analysis indicated that age (OR=1.082, 95%CI: 1.053~1.113, P < 0.05), primary infertility (OR=2.951, 95%CI: 1.990~4.376, P < 0.05), and elevated basal estrogen (OR=1.003, 95%CI: 1.001~1.005, P < 0.05) were risk factors for EPs.The postoperative biochemical pregnancy rate (59.28%vs 52.70%), clinical pregnancy rate (53.09%vs 45.48%), and live birth rate (43.81%vs 35.82%) were significantly higher in the matched EPs group than the non-EPs group (P < 0.05).No statistical difference was observed in pregnancy outcome in the patients with different polyp locations and sizes.The patients with multiple polyps had an obvious higher rate of early miscarriage than those with single polyp (17.27%vs 7.29%, P < 0.05), while those with recurrent polyps also had a higher rate of early miscarriage than those with primary polyps (27.78%vs 11.23%, P < 0.05). Conclusion Age, primary infertility, and elevated basal estrogen are risk factors for EPs in infertility patients, while hysteroscopic endometrial polypectomy prior to embryo transfer results in improved pregnancy outcomes in those with EPs.The location and size of endometrial polyps have weak impact on pregnancy outcomes following embryo transfer, but, the presence of multiple or recurrent polyps may elevate the risk of early miscarriage.

Yu-Chi Tu,&ast; Yu-Mei Wang,&ast; Li-Jun Yao Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Li-Jun Yao, Email ljyao@hust.edu.cnAbstract: DNA can be used for precise construction of complex and flexible micro-nanostructures, including DNA origami, frame nucleic acids, and DNA hydrogels. DNA nanomaterials have good biocompatibility and can enter macrophages via scavenger receptor-mediated endocytosis. DNA nanomaterials can be uniquely and flexibly designed to ensure efficient uptake by macrophages, which represents a novel strategy to regulate macrophage function. With the development of nanotechnology, major advances have been made in the design and manufacturing of DNA nanomaterials for clinical therapy. In diseases accompanied by macrophage disturbances including tumor, infectious diseases, arthritis, fibrosis, acute lung injury, and atherosclerosis, DNA nanomaterials received considerable attention as potential treatments. However, we lack sufficient information to guarantee precise targeting of macrophages by DNA nanomaterials, which precludes their therapeutic applications. In this review, we summarize recent studies of macrophage-targeting DNA nanomaterials and discuss the limitations and challenges of this approach with regard to its potential use as a biological therapy.Keywords: macrophages, DNA nanomaterials, therapy, macrophage-related diseases

This study estimates the effect of macroeconomic and control factors on the capital adequacy ratio of commercial banks in Vietnam. Using the feasible generalized least squares method (FGLS), the following factors are statistically significant in affecting the capital adequacy ratio: national governance quality, economic growth, COVID-19, bank size, liquidity, and leverage. This study also highlights the role of compliance in maintaining capital adequacy during a global crisis, such as the COVID-19 outbreak, because commercial banks need more capital to absorb shocks in the financial instability period caused by the pandemic. Besides, the author emphasizes that in developing countries, especially Vietnam, the government needs to ensure national governance quality, such as political stability and regulatory quality, to increase additional capital buffers to protect them from losses or bankruptcies. Furthermore, the author conducts robustness tests to enhance the reliability and impartiality of the research findings. Doi: 10.28991/ESJ-2024-08-02-010 Full Text: PDF

ZnO-Bi2O3-Co2O3-NiO-Mn3O4-SiO2-Cr2O3 varistor ceramics were prepared by solid-phase sintering method.The effects of different doping amounts of Cr2O3 on the microstructure and electrical properties of ZnO varistor ceramics were investigated, and the phases, microstructures and electrical properties of samples were characterized. The results indicate that the addition of Cr2O3 effectively inhibits the abnormal growth of ZnO grains, enhances the uniformity of grain distribution, reduces the ZnO grain resistance and increases the grain boundary resistance.In the range of 0%-0.21% (molar fraction, the same below) of Cr2O3 addition, the nonlinear coefficient of the varistor ceramics increases first and then decreases as the addition amount increases. When the Cr2O3 doping amount is 0.14%, the ZnO varistor ceramics exhibit excellent electrical properties, with a potential gradient E1 mA=216 V·mm-1, leakage current JL=0.36 μA·cm-2, nonlinear coefficient α=25, residual voltage ratio K=1.815, and aging coefficient Kct=0.647. In addition, the initial U1 mA value of varistor ceramics still maintains 96.75% after impacted by 100 kA pulse current twice with 4/10 μs waveform. The Cr2O3 doped ZnO varistor ceramics demonstrate excellent impulse stability and significant application potential for lightning arresters of power distribution systems.

Bennan Zhao,1,&ast; Qingxiao Liu,2,&ast; Qing Du,3 Jun Kang,1 Rong Tang,4 Yalan Tu,5 Dafeng Liu1 1The First Ward of Internal Medicine, Public Health Clinical Center of Chengdu, Chengdu, People’s Republic of China; 2Department of Neurology, Hospital of Chengdu University of TCM, Chengdu, People’s Republic of China; 3The Second Ward of ICU, Public Health Clinical Center of Chengdu, Chengdu, People’s Republic of China; 4Department of Integrated Chinese and Western Medicine, Public Health Clinical Center of Chengdu, Chengdu, People’s Republic of China; 5Scientific Research and Teaching Department, Public Health Clinical Center of Chengdu, Chengdu, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Dafeng Liu, The First Ward of Internal Medicine, Public Health Clinical Center of Chengdu, Chengdu, People’s Republic of China, Tel +86 1 309 449 4836, Email ldf312@126.com Yalan Tu, Scientific Research and Teaching Department, Public Health Clinical Center of Chengdu, Chengdu, People’s Republic of China, Tel +86 1 501 326 6454, Email tyl950330@163.comPurpose: To date, there are few reports about mpox case series in China, and scarce information is available about the in-vivo kinetics of T-cell responses in the early stage of mpox infection. This study aims to investigate the clinical difference among mpox patients with and without human immunodeficiency virus (HIV) infection.Patients and Methods: A total of 56 patients diagnosed with mpox by Chengdu Center for Disease Control and Prevention (CDC) and hospitalized in Public Health Clinical Center of Chengdu were retrospectively included and divided into an HIV-infected group (n=23) and a non-HIV-infected group (n=33). Clinical characteristics and serum chemistry findings of mpox patients were collected in order to analyze the differences between the HIV-infected group and the non-HIV-infected group.Results: Multiple laboratory abnormalities, including elevated C-reactive protein (69.1%), hypocalcemia (50.9%), elevated CD3+CD8+T counts (47.0%) and inverted ratio of CD3+CD4+T to CD3+CD8+T (64.7%) were common in mpox cases. There were statistically significant differences (all P < 0.05) in age, serum calcium levels, CD3+CD4+T counts, the ratio of CD3+CD4+T to CD3+CD8+T, proportion with > 10 rashes, incidence of proctitis anus and time from rash growth to rash scab shedding between HIV-infected group and non-HIV-infected group. In the early stage of mpox infection, the median of CD3+CD8+T counts in the non-HIV-infected group was significantly higher than that in healthy donors (P< 0.001), and the median of CD3+CD4+T/CD3+CD8+T ratio was significantly lower (P< 0.001). The median of CD3+CD4+T counts in mpox patients co-infected with HIV significantly decreased compared to the pre-infection level (p =0.033).Conclusion: Our study indicates that mpox co-infected with HIV patients have longer lasting rash lesions and a higher incidence of proctitis anus. T-cell responses may be different between HIV-infected and non-HIV-infected individuals in the early stage of mpox infection.Keywords: mpox, HIV, clinical characteristics, complication, T-cell response

Background: Multisystem inflammatory syndrome in children (MIS-C) is a novel disease associated with COVID-19. The COVID-19 epidemic peaked in May 2022 in Taiwan, and we encountered our first case of MIS-C in late May 2022. We aimed to present patients’ clinical manifestations and identify risk factors for shock. Methods: We included patients diagnosed with MIS-C at two medical centers from May 2022 to August 2022. We separated those patients into two groups according to whether they experienced shock. We collected demographic, clinical manifestation, and laboratory data of the patients and performed statistical analysis between the two groups. Results: We enrolled 28 patients, including 13 (46 %) with shock and 15 (54 %) without shock. The median age was 6.4 years (IQR: 1.9–7.5). In single variable analysis, patients with shock tended to be older, had more neurological symptoms, more conjunctivitis and strawberry tongue, lower lymphocyte count, lower platelet counts, and higher C-reactive protein, higher procalcitonin, higher ferritin, and higher D-dimer levels than those without shock. The area under the ROC curve that used procalcitonin to be the risk factor of shock with MIS-C was 0.815 (95 % CI 0.644 to 0.987). The cutoff value obtained by ROC analysis of procalcitonin was 1.68 ng/mL. With this cutoff, the test characteristics of procalcitonin were as follows: sensitivity 77 %, specificity 93 %, positive predictive value 91 %, negative predictive value 82 %. Multivariable analysis revealed that procalcitonin was the only independent risk factor of shock with MIS-C on admission (OR, 26.00, 95 % CI, 1.01–668.89). Conclusions: MIS-C patients with high initial procalcitonin levels have higher risks of experiencing shock and may need ICU admission.

Abstract Chlorella sp. is able to grow and transform inorganic and organic contaminants in wastewater to create biomass. In the present study, Chlorella sp. LH2 isolated from cocoon wastewater was able to thrive in hospital wastewater, then remove nutrients and eliminate E. coli ATCC 8739. The results indicated that optimal cultivation conditions of Chlorella sp. LH2 in hospital wastewater were pH of 8, light:dark cycle of 16:8 at 30oC. The inhibitory effect of chlorination on algae growth was accompanied with the chlorine concentration. BOD5:COD ratio of 0.77 indicated biodegradability of hospital wastewater. The untreated and treated wastewatee samples were collected to investigated the nutrient removal efficiency after 10 days. Untreated and treated results were192 ± 8.62 mg/l 23.91 ± 2.19 mg/l for BOD5; 245 ± 9.15 mg/l and 47.31 ± 5.71 mg/l for COD. The treated value met the required standards for hospital wastewater treatment. The removal efficiency total nitrogen and total phosphorus were 68.64% and 64.44% after 10 days, respectively. Elimination of E. coli ATCC 8739 after 7 days by Chlorella sp. LH2 was 88.92%. The results of this study suggest the nutrients and pathogens removal potential of Chlorella sp. LH2 in hospital wastewater for further practical applications.