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The aim of this research was to know the effect of maturity of red coconut water as a diluent to the sperms quality of Boer goats during chilled preservation. The research was carried out at Laboratory Sumber Sekar Faculty of Animal Husbandry University of Brawijaya Malang, on 11st December 2016 until 31st January 2017. The method used in this research was laboratory experimental using Randomized Block Design with three treatments, P0 (Tris Aminomethane + 20% Egg Yolk), P1 (Young Red Coconut Water + 20% Egg Yolk) and P2 (Mature Red Coconut Water + 20% Egg Yolk) which each treatment using 10 repetitions. The observed variables include the percentage of motility, percentage of viability and percentage of abnormal spermatozoa during chilled preservation The results showed that on the 3ndday the percentage of motility of spermatozoa during chilled preservation gave a significant effect (P 0.05). The highest of total motile spermatozoa was P0 (Tris Aminomethan + 20% Egg Yolk). P2 better than P1 on motility, but not on viability and abnormality. The conclusions was the maturity of red coconut water can improve motility, but can not improve viability and abnormality. The suggested was use Mature Red coconut water + 20% Egg Yolk as a diluent and do further research on coconut water to be able to maintain spermatozoa longer. Keyword : Sperms, egg yolk, red coconut water, tris aminomethane

Testing of spermatozoa fertility in vivo is needed to find out the extent of the ability of spermatozoa fertilization in the female reproductive tract. The fertility ability of spermatozoa in fertilizing the ovum is indicated by the number of fertile eggs from the number of eggs incubated resulting from the mating with Artificial Insemination (AI). The aim of this research is to know the percentage of fertility of eggs from AI using semen which has been stored cold for 8 hours on Ringe's diluent with the addition of antioxidant glutathione. Collection semen with teaser female method is done in Poultry Breeder Installation of Sekolah Tinggi Penyuluhan Pertanian (STPP) Malang. The 30 female chickens were divided into 3 groups of treatment P0: AI using fresh semen with Ringer's diluent, P1: AI using cold cement with Ringer's Diluent (8 hours duration storage) and P2: AI using cold semen with 0.5 mM addition in Diluent Ringe's (8 hours duration storage). Experiment design using Complete Randomized Design (RAL) and nonparametric data analysis with chi-square. The observed variable was egg fertility calculated based on egg fertility percentage. The results showed that the dilution treatment had a significant effect (P

The purpose of this study was to measure the motility of Madura bull spermatozoa using three different diluents (tris aminomethane, CEP-2 and skim milk) duringcold storage. The research material used were 2 Madura bulls. Collecting semen method used artificial vagina, followed by fresh semen analysis and processing of liquid semen. Observation of liquid semen quality was carried out up to the 5th days of cold storage. Motility examination of semen liquid used Computerized Assisted Semen Analysis (CASA), namely SCA v.5.2. The parameters measured were: progressive motility, motility, velocity straight linear (VSL), velocity curve linear (VCL), velocity average pathway (VAP), straightness (STR), linearity (LIN), wobble (WOB), hyperactivity (H), Beat cross frequency (BCF), amplitude lateral head (ALH). Data was analyzed by Minitab 17. Motility and progressive motility of spermatozoa in tris aminomethane and CEP-2 were higher than skim milk for 5 days of cold storage. The VCL value of tris aminomethane was higher than CEP-2 and skim milk. VSL scores barely show the difference among diluents. VAP values in tris aminomethaneand CEP-2 are higher than skim milk at the beginning of storage. The LIN, STR and WOB values of the CEP-2 and skim milk yield higher values than tris aminomethane. The percentage value of hyperactivity spermatozoa was same among diluents. The ALH value of trisaminomethane was higher than CEP-2 and skim milk. BCF values in CEP-2 was higher than skim milk and tris aminomethane. Tris aminomethane and CEP-2 can support the motility of Madura bull spermatozoa than skim milkfor 5 days of storage

Penelitian in bertujuan untuk mengetahui pengaruh lama sentrifugasi terhadap kualitas dan proporsi spermatozoa X-Y sapi limousin hasil sexing dengan gardien densitas percoll menggunakan pengencer CEP-2 + 10% kuning telur. Metode penelitian yang digunakan adalah metode percobaan. Dalam penelitian ini percobaan dilakukan dengan 2 perlakuan terdiri dari lama sentrifugasi 5 menit dan 7 menit menggunakan kecepatan yang sama yaitu 2250 rpm (850 G). Hasil penelitian menunjukkan perlakuan sentrifugasi 5 menit memiliki kualitas yang lebih baik dari 7 menit meliputi konsentrasi, viabilitas dan total spermatozoa motil, sedangkan abnormalitas dan motililtas lebih baik sentrifugasi 7 menit dari pada 5 menit. Proporsi spermatozoa X pada perlakuan sentrifugasi 5 dan 7 menit tidak berbeda nyata (P>0,05) yaitu 78,6% dan 76,5%. Kata kunci : Sentrifugasi, Sexing Gradien Densitas Percoll, Kualitas Spermatozoa, Proporsi Spermatozoa, CEP-2+10%KT

Penelitian ini bertujuan untuk mengetahui pengaruh penggantian Bovine Serum Albumin (BSA) menggunakan serum darah sapi pada pengencer dasar Cauda Epididymal Plasma 2 (CEP-2) terhadap kualitas semen sapi Limousin selama pendinginan pada suhu 3-5oC. Materi penelitian yang digunakan yaitu semen segar dari sapi Limousin yang dipelihara di Balai Besar Inseminasi Buatan Singosari Malang. Serum darah didapatkan dari darah sapi yang diambil dari Laboratorium Lapang Sumber Sekar Fakultas Peternakan Universitas Brawijaya. Metode yang digunakan dalam penelitian menggunakan percobaan laboratorium dengan Rancangan Acak Kelompok yang terdiri dari lima perlakuan, yaitu P0 sebagai kontrol (90% CEP-2 (dengan BSA) + 10% KT); P1 (83,84% CEP-2 + 6,16% serum + 10% KT); P2 (81,84% CEP-2 + 8,16% serum + 10% KT); P3 (79,84% CEP-2 + 10,16% serum + 10% KT) dan P4 (90% CEP-2 (tanpa BSA) + 10% KT). Parameter yang diamati adalah motilitas, viabilitas dan abnormalitas. Hasil penelitian, persentase motilitas yang tertinggi yaitu P0 (37,6±17,3%). Persentase viabilitas yang tertinggi yaitu P3 (63,4±3,5). Persentase abnormalitas yang tertinggi yaitu P0 (16,4±4,0%). Total spermatozoa motil pada semua perlakuan lebih besar daripada kriteria Standar Nasional Indonesia (SNI) yaitu 40% spermatozoa motil dari total konsentrasi. Disimpulkan bahwa serum darah belum mampu menggantikan BSA pada CEP-2, namun pada pengencer dengan serum 6,16% cukup mampu mempertahankan motilitas spermatozoa walaupun tidak sebaik pengencer dengan BSA. Kata kunci : semen cair, penyimpanan dingin, motilitas, viabilitas, abnormalitas

Tujuan dari penelitihan ini adalah pengaruh penggunaan CEP-2 dengan suplementasi kuning telur pada berbagai level terhadap kualitas spermatozoa sapi FH kualitas rendah selama penyimpanan pada suhu 4-5 oC. Penelitian ini dilakukan di Laboratorium Reproduksi Ternak Fakultas Peternakan Universitas Brawijaya, tanggal 17 September hingga 18 November 2014. Metodologi yang digunakan adalah percoboaan menggunakan rancangan acak kelompok (RAK). Penelitihan ini menggunakan 3 perlakukan, yaitu (P1 = CEP-2 + 10% Kuning Telur, P2 = CEP-2 + 15% Kuning Telur, P3 = CEP-2 + 20% Kuning Telur) dengan 10 ulangan. Hasil penelitihan menunjukkan bahwa pengencer CEP-2 dengan suplementasi 10%, 15%, 20% kuning telur tidak berpengaruh secara signifikan (P> 0,05) terhadap persentase motilitas, viabilitas dan abnormalitas spermatozoa sapi FH yang memiliki kulitas rendah. Semen segar kualitas rendah dalam pengencer CEP-2 yang ditambahkan 10%, 15% dan 20% kuning telur dapat bertahan sampai hari ke 1 untuk dapat digunakan dalam proses IB. Disarankan untuk memanfaatkan semen segar sapi FH yang memiliki kualitas rendah untuk inseminasi buatan. Kata kunci: semen, pengencer, motilitas, viabilitas, abnormalitas.

The purpose of this research was to know about the successfully artificial insemination (AI) using frozen and liquid semen of Ongole cross (PO) cattle indicated by Service per Conception (S/C), Days Open (DO) dan Conception Rate (CR). The materials used were 60 heads of Ongole crossbred cattle which were selected purposively using some criteria, such as having calving experience, healthy, and free from any reproduction disorder. This field experiment consisted of direct observation and interview to acquire primary and secondary data. Datas were analyzed descriptively and continued by a paired t-test. The results showed that the value of (S/C) was very significantly different (P0.05). CR for P1, P2, P3, and P4 was found 63.33% , 86.67%, 16.33%, 83.33% respectively. The conclusion of this research was AI using frozen and liquid semen with only 5 days preservation had a difference on the value of service per conception. The most successfull AI was found higher in the liquid semen which was stored for 1 and 5 days than that of frozen semen. Key words : semen, service per conception, days open, calving interval

The purpose of this research was to know the effect of castration to Wagyu crossbreed cattle production performance based on age difference and know the appropriate age to do castration. The materials used in this research were 37 heads of 3-month old and 28 heads 4 months calf castration. The method was case studies with purposive sampling. Data were analyzed using unpaired t test. The results showed that the calf castration age of 3 months and 4 months showed significantly different (P

The purpose of this research was to analyse the quality and storability of native chicken semen at room temperature after diluted with physiological saline solution (NaCl) supplemented with noni fruit extract. Research methodology used was nested randomized design which variables observed covering the quality of chicken spermatozoa macroscopically and microscopically with the doses of noni fruit extract of 0 % (P0), 10 % (P1), 20 % (P2), and 30 % (P3). Rate of spermatozoa motility at 0, 1, 2, and 3 hours on P0 were 83.70±4.74, 63.30±9.63, 32.90±8.52, and 15.00±6.20; P1 were 85.30±5.44, 72.70±10.06, 51.90±11.75, and 28.50±5.68; P2 were 84.60±6.40, 78.50±8.59, 57.90±10.73, and 33.70±9.06; and P3 were 81.80±7.30, 64.20±7.93, 40.30±11.66, and 19.80±7.47, respectively. Rate of spermatozoa viability at 0, 1, 2, and 3 hours on P0 were 85.30±9.09, 65.10±6.15, 32.10±11.86, and 10.30±9.09; P1 wer 87.40±6.22, 72.70±5.33, 50.80±13.59, and 26.50±10.99; P2 were 68.58±5.30, 77.70±4.79, 56.30±13.76, and 32.70±13.79; P3 were 81.20±8.04, 68.70±10.40, 36.20±16.61, and 16.90±11.93, respectively. Rate of spermatozoa abnormality at 0, 1, 2, and 3 hours on P0 were 8.70±3.40, 12.20±4.42, 17.80±5.67, and 20.30±6.38; P1 were 7.80±2.04, 9.80±2.69, 13.60±4.45, and 16.50±5.19; P2 wer 8.40±4.33, 10.00±2.45, 12.50±5.21, and 15.80±3.71; and P3 were 9.60±3.41, 10.90±2.64, 17.10±5.61, and 21.20±8.16, respectively. It can be concluded that the addition of noni fruit extract at the dose of 10 % and 20 % in physiological saline solutin maintain the native chicken spermatozoa quality up to 2 hours post storage at room temperature.

This study aims to determine the effect of the substitution of bovine serum albumin (BSA) with cattle blood serum and egg white in the diluent of Cauda epididymal Plasma 2 (CEP-2) on sperm quality of Limousin cattle during cooling at 3-5 C. The research material used was rejected Limousin bull sperm (motility of 50-60%) from Artificial Insemination Centre Singosari, Malang. This research was a laboratory experiment using a randomized block design which was composed of six treatments with 10 replications, those were T0 as controls ((90% CEP-2 with BSA + 10% egg yolk); T1 (83.84% CEP-2 + 6.16% cattle blood serum + 10% egg yolk); T2 (81.84% CEP-2 + 8.16% cattle blood serum + 10% egg yolk); dan T3 (90% CEP-2 + 0,4% egg white + 10% egg yolk); T4 (90% CEP-2 + 0.8% egg white + 10% egg yolk); and T5 (90% CEP-2 without BSA + 10% egg yolk). Parameters measured were the percentage of motility, viability, and abnormality of sperms. Results of research after 48 hours of storage showed that the percentage of sperm motility in T0, T1, T2, T3, T4, and T5 were 40.50±5.90, 36±36.16, 34.00±6.58, 40.50±3.69, 38.50±3.37, and 38.50±4.12, respectively, while the percentage of sperms viability were 75.16±4.30, 70.50±2.88, 73.80±2.80, 74.80±3.30, 75.13±3.13, and 74.03±4.13, respectively, and the percentage of sperms abnormality were 10.14±2.34, 10.62±1.34, 11.33±2.00, 10.94±2.82, 10.02±1.95, and 10.78±1.96, respectively. In conclusion, CEP-2 diluent with or without the addition of 19% egg yolk in BSA and the substitution of BSA with 0.4-0.8% egg white can maintain semen quality to hour of 48 in cold storage.This study aims to determine the effect of the substitution of bovine serum albumin (BSA) with cattle blood serum and egg white in the diluent of Cauda epididymal Plasma 2 (CEP-2) on sperm quality of Limousin cattle during cooling at 3-5 C. The research material used was rejected Limousin bull sperm (motility of 50-60%) from Artificial Insemination Centre Singosari, Malang. This research was a laboratory experiment using a randomized block design which was composed of six treatments with 10 replications, those were T0 as controls ((90% CEP-2 with BSA + 10% egg yolk); T1 (83.84% CEP-2 + 6.16% cattle blood serum + 10% egg yolk); T2 (81.84% CEP-2 + 8.16% cattle blood serum + 10% egg yolk); dan T3 (90% CEP-2 + 0,4% egg white + 10% egg yolk); T4 (90% CEP-2 + 0.8% egg white + 10% egg yolk); and T5 (90% CEP-2 without BSA + 10% egg yolk). Parameters measured were the percentage of motility, viability, and abnormality of sperms. Results of research after 48 hours of storage showed that the percentage of sperm motility in T0, T1, T2, T3, T4, and T5 were 40.50±5.90, 36±36.16, 34.00±6.58, 40.50±3.69, 38.50±3.37, and 38.50±4.12, respectively, while the percentage of sperms viability were 75.16±4.30, 70.50±2.88, 73.80±2.80, 74.80±3.30, 75.13±3.13, and 74.03±4.13, respectively, and the percentage of sperms abnormality were 10.14±2.34, 10.62±1.34, 11.33±2.00, 10.94±2.82, 10.02±1.95, and 10.78±1.96, respectively. In conclusion, CEP-2 diluent with or without the addition of 19% egg yolk in BSA and the substitution of BSA with 0.4-0.8% egg white can maintain semen quality to hour of 48 in cold storage.

Penelitian ini bertujuan untuk mengetahui pengaruh lama ekuilibrasi semen sapi Wagyu terhadap kualitas semen dan untuk mengetahui pengaruh individu sapi Wagyu terhadap kualitas semen pada lama ekuilibrasi yang berbeda. Materi yang digunakan berupa semen segar sapi Wagyu yang berasal dari 9 kali proses penampungan dari 3 ekor sapi. Proses penampungan dilakukan 1 kali seminggu setiap sapi dengan menggunakan vagina buatan. Sapi dipelihara dengan manajemen yang baik di unit pembibitan PT. Austasia Stockfeed. Semen segar yang digunakan memiliki nilai rata-rata persentase motilitas individu 75% dan motilitas massa 3+. Pengencer yang digunakan adalah AndromedÒ. Metode penelitian ini eksperimental laboratorium dengan 3 perlakuan dan 10 ulangan. Perlakuan yang diberikan tiga rentang waktu lama ekuilibrasi semen dengan P1 (3 jam 30 menit), P2 (4 jam), dan P3 (4 jam 30 menit). Variabel yang diamati diantaranya persentase motilitas individu, pada saat sebelum pembekuan (before freezing) dan setelah pembekuan (post thawing) serta total spermatozoa motil (TSM). Analisis data menggunakan analisis ragam, dan rancangan yang digunakan adalah Rancangan Acak Kelompok (RAK) tersarang dua tahap. Hasil penelitian ini menunjukkan bahwa perbedaan lama ekuilibrasi (P1, P2, dan P3) tidak memberikan pengaruh nyata (P>0,05) terhadap persentase motilitas individu spermatozoa sapi Wagyu. Perbedaan individu bull sapi Wagyu memberikan pengaruh terhadap kualitas semen before freezing dan post thawing. Individu bull 1 memiliki nilai persentase kualitas spermatozoa terbaik dengan nilai persentase motilitas individu spermatozoa before freezing dan post thawing dengan nilai 61,67±2,50% dan 35,51±7,71%. Total spermatozoa motil terbaik pada lama ekuilibrasi 4,5 jam (P3) 14,0±1,0 juta spermatozoa/straw. Kata kunci: kualitas semen, motilitas individu, post thawing

The purpose of this research was to determine duration effect of different glicerolitation time on the quality and frozen semen production of Simmental bulls in Ungaran Artificial Insemination Center. The materials of the research used four Simmental bulls. The method of the study used experimental laboratory. There were two treatments in the study (P0 = duration of glicerolitation for > 3 hours, P1 = duration of gliserolitation for 15 minutes) and four Simmental bulls. The research parameter included semen volume, pH, concentration of spermatozoa, frozen semen production and post thawing motility from 8 treatments. Data were analyzed using t-test. The quality of fresh semen had average percentage of motility 70% and 2+. The research showed that the frozen semen production during 15 minutes of glicerolitation was better than that of >3 hour duration. Moreover, the motile sperms produced during 15 minutes of glicerolitation was higher than those which produced during >3 hours of glicerolitation. Keywords: motility, glicerolitation, frozen semen

Purpose of this research was to investigate the effect of Bovine Serum Albumin substitution by albumen on CEP-2 to semen quality Ongole CrossBred bull stored at 3-5oC. Research was conducted at Research Centre Beef Cattle Laboratory, Grati, Pasuruan on February 2016. Semen diluent was divided into two groups, there were P0 (90% CEP-2 + 10% Egg Yolk (EY)); and P1 (90% CEP + 0.4% albumen + 10% EY). Data of the research were analyzed using paired design t test. The result showed that after eight days chilled preservation, percentage of motility P1 (47.4±10.9%) was higher than P0 (47±9.2%). Percentage of viability P0 (83.1±1.9%) was higher than P1 (81.3±1.5%). Percentage of abnormality P1 (3.6±0.4%) was lower than P0 (3.8±0.3%). Total motile sperm count after six days chilled preservation was significantly higher in all treatments compared to the standard criteria of SNI 40% motile sperm/ml. The conclusion of this research was 0,4% albumen could replace the BSA capability on CEP-2. Keywords: CEP-2, Bovine Serum Albumin, chilled preservation, semen quality

The purposes of this study were determine the carcass characteristics and physical meat quality of Brahman Cross Steer on different rest periods. The experiment was conducted on June 2015 at Slaughter house Cianjur Arta Makmur Ltd. in the Cianjur District, West Java. The total number of cattle used in the study was 30 heads of Brahman Cross Steer treated for 18 hours lairage duration and 30 heads of Brahman Cross Steer treated for 3 hours lairage duration from Pasir Tengah feedloter Ltd (Holding Widodo Makmur Perkasa Ltd). The method used a case study. Data were analyzed using unpaired t test. The results showed that the carcass characteristics including slaughtered weight, hot carcass weight, chilled carcass weight and carcass weight loss percentage were not significantly different, while the dressing percentage for 18 hours lairage duration (54.16±1.62%) and 3 hours lairage duration (55.60±2.00%) was significantly different (P

Penelitian ini bertujuan untuk mengkaji dan mengidentifikasi perbedaan kecepatan pubertas calon pejantan sapi PO yang dipelihara pada kelompok sex yang berbeda. Materi yang dipergunakan 28 ekor pedet jantan dan 9 ekor pedet betina dengan umur 8 - 11 bulan, yang dibagi menjadi tiga perlakuan A (kontrol) yaitu satu ekor sapi jantan/kandang ditempatkan dalam kandang individu; B yaitu empat ekor sapi jantan (sex sejenis ditempat dalam kandang kelompok) dan C yaitu empat ekor sapi jantan dan tiga ekor sapi betina muda (sex beda jenis yang ditempatkan dalam kandang kelompok) dengan tiga ulangan. Kegiatan ini dilaksanakan enam bulan di kandang percobaan Loka Penelitian Sapi Potong, Grati, Pasuruan. Data yang diperoleh dianalisa dengan Two Way Anova menggunakan program Microsoft Excel 2007. Hasil penampungan semen dengan elektroejakulator menunjukkan spermatozoa dengan persentase motilitas individu minimal 10 % dan konsentrasi minimal 50 juta/ml yaitu sapi kandang A = 1 ekor; B= 4 ekor dan C=3 ekor. Hasil analisis ragam terhadap hasil penampungan semen tidak berbeda nyata (P>0,05) dari ketiga perlakuan. Kesimpulan dari penelitian ini menunjukkan bahwa calon pejantan sapi PO yang dipelihara pada kelompok sex sejenis maupun beda jenis memiliki waktu pubertas yang sama cepatnya (15 bulan) dibandingkan pada kandang individu (16 bulan). Kata Kunci : Calon Pejantan sapi PO, kelompok sex dan pubertas

Penelitian ini bertujuan untuk mengetahui perbedaan produksi semen beku pada berbagai bangsa sapi potong dan untuk mengetahui produksi semen pada masing-masing individu pada bangsa yang sama. Penelitian dilakukan di Balai Inseminasi Buatan Daerah Ungaran Jawa Tengah Indonesia. Materi yang dipergunakan dalam penelitian ini adalah catatan produksi semen yang terdiri kualitas semen segar dan jumlah straw semen beku pada bulan Januari sampai Desember tahun 2014 sebanyak 39 ekor sapi yang terdiri dari 18 ekor sapi Simental, 8 ekor sapi Limousin, 8 ekor PO dan 5 ekor sapi Brahman. Pengencer yang dipergunakan adalah skim kuning telur. Metode yang dipergunakan adalah studi kasus dengan rancangan acak kelompok. Variabel yang diamati adalah Volume semen, Konsentrasi, Motilitas individu, total spermatozoa dan total spermatozoa yang motil. Hasilnya menunjukkan volume sapi Limousin yang terbanyak = 7,2 + 1,3 ml, diikuti oleh oleh sapi simental = 6,8 + 1,6 ml, sapi PO = 6,1 + 1,2 ml dan yang paling sedikit adalah sapi Brahman = 4,2 + 1,8 ml. Rata-rata volume semen pada berbagai bangsa terdapat perbedaan yang nyata (P0,05). Rata-rata Total spermatozoa dan total spermatozoa yang motil pada sapi Simental adalah 9.320,3 + 2.461,2 and 5.532,9 + 2.314,2 ; Sapi Limousin = 11.266,9 + 1.159,6 dan 7.908,9 + 3.851,2; Sapi PO = 8.995,1 + 2.860,8 dan 5.651,6 + 2.418,4 , Sapi Brahman = 6.189,3 + 22,6 dan 3.053,3 + 4.356,6. Rata-rata kedua parameter ini tidak menunjukkan perbedaan yang nyata pada berbagai bangsa sapi (P>0,05). Rata-rata produksi semen beku per hari pada sapi Simental 280,67 + 68,57 straw ; sapi Limousin 315,6 + 44,32 straw ; sapi PO 275,15 +70,61 straw dan sapi Brahman 225,18 + 59,74 straw. Rata-rata produksi semen beku tidak menunjukkan perbedaan yang nyata pada berbagai bangsa sapi (P>0,05). Volume semen, motilitas individu dan produksi semen beku harian masing-masing individu pada bangsa yang sama pada sapi Limousin, Simental, PO dan Brahman terdapat perbedaan yang sangat nyata (P

The purpose of this research was to determine semen quality and frozen semen production of beef cattle at different month collection in Lembang Artificial Insemination Center. Beef cattle strain that used for this research are Aceh, Angus, Brahman, Limousine, Madura, Ongole and Simmental. The research parameter included semen volume, pH, motility of spermatozoa, concentration of spermatozoa, number of spermatozoa, number of spermatozoa motil and number of frozen semen straw. The method of this research using factorial completely Randomized Design and each had 3 replication per treatment. The different of beef cattle have given a significant effect of semen volume (P0.05). Different month collection have a significant influence of semen volume (P

Penelitian dilakukan di Balai Inseminasi Buatan Daerah Ungaran Jawa Tengah. Tujuan penelitian untuk mengetahui perbedaan kualitas semen segar dan produksi semen beku sapi Simmental pada umur yang berbeda. Materi yang digunakan dalam penelitian ini adalah catatan produksi semen yang terdiri dari kualitas semen segar dan jumlah straw semen beku dari 8 ekor sapi Simmental. Metode yang digunakan adalah studi kasus dengan Nested Design. Variabel yang diamati meliputi volume semen, pH, motilitas massa, persentase motilitas individu, konsentrasi spermatozoa, total spermatozoa, total spermatozoa motil dan jumlah straw semen beku. Hasil penelitian menunjukkanbahwa kualitas dan kuantitas semen segar pada 4 kelompok umur 3, 4, 7 dan 8 tahun secara berturut-turut adalah volume: 6,9±1,7; 7,3±0,5; 7,8±0,3 dan 9,2±1,6 ml, pH: 6,4±0,1; 6,5±0,1; 6,5±0,1 dan 6,5±0,1, motilitas massa: 2±0,04; 2±0; 2±0,05 dan 1,8±0,22 (+), persentase motilitas individu: 72.1±1.2; 71.3±0.8; 70.3±0.5 dan 66.3±5%, konsentrasi spermatozoa: 1.703,3±167,2; 1.612,2±172,2; 1.450,7±172,2 dan 1.254,6±113,6 (juta/ml), total spermatozoa: 11.586,5±1945,6; 10.468,1±1148,8; 12.593,6±947,8 dan 11.570,2±2642,2 juta sel, total spermatozoa motil: 8.341,8±1.282,5; 7.471,7±845,6; 8.857,6±662,7 dan 7.820,3±2.229,4 juta sel, jumlah straw semen beku: 300±38,2; 263,6±57,8 dan 236,6±180,1 buah straw. Kata kunci: semen beku, motilitas, konsentrasi, spermatozoa motil

The aim of this study was to determine the distribution of beef carcass component from Brahman Cross cattle at different slaughtered age. The number of cattle was 99 head (49 steers and 50 heifers), the body weight 350 - 500 kg and age ranged PI0 - PI2 classess. The cattle were raised in PT. Widodo Makmur Perkasa and then slaughtered at slaughter house in Cianjur District, West Java. Accidental sampling method was applied to the research. A direct observation was done to obtain information about age, sex, body weight and carcass weight and its part (meat, bone and fat). The data were analyzed using independent t-test, correlation and linear regresion. The result showed that the average slaughtered weight and carcas weight of Brahman Cross heifers was higher than that of Brahman Cross steers at PI0 sex-class (P0.05). Average meat percentage of heifers and steers was also relatively similar (73.39% : 73.65%). Average bone percentage of steers was higher than that of heifers (14.82% : 14.33 %). Whereas, average fat percentage of heifers was higher than that of steers (11.00% : 10.21%). The research concluded that steers of Brahman Cross cattle at all age-classess had better distribution of carcass component than heifers in the same age-classess. Steers of Brahman Cross cattle at PI2 slaughtered age-class had an optimal distribution of carcass components. Keyword: Brahman Cross, slaughtered age, sex-class, distribution, carcas component.

The purpose of this study was to evaluate the implementation of Artificial Insemination using sexed frozen semen processed with percol density gradient sentrifugation method on service per conception (S/C), Non Return Rate (NRR) and Conception Rate (CR) of Ongole crossbred cattle. The material used in this study were 54 Ongole crossbred cattle which were purposively selected with criteria had conditions of estrus 2A+. Fifty five cattle were divided into 2 treatments such as P0 (non sexed semen) with Post Thawing Motility (PTM) quality 40% and P1 (sexed semen) with PTM quality 5-10%. The study was conducted in the village Boro Bunut and Boro Kemantren Pakis subdistrict Malang. The study showed that P0 (non sexed semen) had S/C 1.3, NRR0-21 74.07%, NRR22-42 74.07%, NRR43-63 74.07% and CR 74.07%, while P1 (sexed semen) had S/C 1.5, NRR0-21 74.07%, NRR22-42 59.25%, NRR43-63 59.25% and CR 55.56%. These results indicated that P0 (non-sexed semen) had better results than P1 (sexed semen), but statistically the two treatments were not significantly different (P>0.05). The highest percentage of cattle pregnancy (74.07%) was found in P0 (non sexed semen) as compared to P1 (sexed semen) which was 59.25%. Keywords: Sexing, estrus, cattle

The purpose of this research was to determine the success of AI using sexed semen results based on NRR, S/C, CR, and rectal palpation were also determine. The total number of 81 acceptors divided into 3 treatment groups, such as P0 (non- sexed semen) ; P1 (Frozen semen Sexed using White Yolk Sedimentation), P2 (Frozen semen sexed using Percoll Gradient Centrifugation) . The results showed the NRR percentage of P0, P1 and P2 were between the treatments are P0 of 74.07 ± 12:00%; 65.43 ± 5.66% and 64.19 ± 8:56% respectively. Furthermore the highest of CR was in P0 44%, P2 25.91% and P1 18.51%. Conclution of this research was the highest percentage of pregnancy was 59.25 %(P0) , P1 51.85% and the lowest was in 44.44%. The S/C was in (P0) 2.31 follow by 3.00 in P2 and 3.33 in P1. Key words : artificial insemination , frozen semen sexed, the percentage of Gestation and feed

Egg yolk is most commonly used in semen extender for preservation and cryopreservation but potential risk of xenobiotic contamination has raised question upon the use of egg yolk. Therefore, the present study was designed to evaluate soy milk CEP-2 based extender on liquid semen of Limousin during chilled preservation. Semen divided into five groups and diluted at 1/8 (semen/extender) with 7,5%, 10%, 12,5%, 15% soy milk respectively (CEP-2+7,5% SK, CEP-2+10% SK, CEP-2+12,5% SK, CEP-2+15% SK) and 10% egg yolk (CEP-2+10% KT). The results of current study showed egg yolk give the superior effect on maintaining sperm quality (motility, viability and abnormality) after 24 hours in chilled preservation. The motility and viability in CEP-2+Soy Milk groups were significantly (P0,05) on motility percentage between CEP-2+15% SK and CEP-2+10% KT. Furthermore, the abnormality in CEP-2+10% KT was significantly lower than those CEP-2+Soy Milk groups (P

This study was done to evaluate quality of Limousin’s semen during chilled preservation. The characteristic of spermatozoa were easily damaged, so that they were necessary to protect viability of spermatozoa. Addition of extender was conducted to determine quality and viability of spermatozoa. Each extender were added Egg Yolk as diluents cryopreservation, the treatment were devided in to three groups, they were ; P0 (CEP-2 + 10% Egg Yolk), P1 (Tris Aminomethane + 20% EggYolk), P2 (Skim + 10% Egg Yolk). The result showed that Tris Aminomethane + 20% Egg Yolk gave the best effect on maintaining quality of spermatozoa (motility, viability and abnormality). The motility in three extender at 24h was high significant different (P

This research was carried out at Laboratory of Balai Besar Inseminasi Buatan, Singosari, Malang start from 8th May until 27th May 2014. Research was to find the effect of liquid semen of Limousin during chilled preservation. Semen divided into four with 5%, 10%, 15%, coconut milk (P1=CEP-2+5% S, P2=CEP-2+10% S, P3=CEP-2+15% S) and 10% egg yolk (P0=CEP-2+10% KT), gave the best result in P0 and has highly significant different (P

Bagian Produksi ternak Fakultas Peternakan Universitas Brawijaya

The purpose of this study was to evaluate artificial insemination program on the reproduction performance of Ongole crossbred and Limousin crossbred cattle. The materials of the study were Ongole crossbred and Limousin crossbred cattle. Descriptive analysis was used to determine service per conception (S/C), days open (DO), calving interval (CI), conception rate (CR) and calving rate (CvR). Meanwhile, t-test was used to analyse differences among those variables. The study showed that the value of S/C, DO and CI between Ongole crossbred and Limousin crossbreed cattle differed significantly (P

The study was conducted in subdistrict Pakis, Malang Regency from May to July 2013. The study materials were 54 Ongole crossbred cattle. Twenty seven cattle were inseminated using non sexed semen (P0) and 27 cattle were inseminated using sexed semen (P1). Samples were selected randomly using criterion that semen deposition was in position 4+. Gestation evaluation was calculated based on non return rate (NRR), service per conception (S/C) and conception rate (CR). The study showed that P0 had 74% of NRR and P1 had 59% of NRR. S/C was found 1.37 at P0 and 1.48 at P1. CR was found 74% at P0 and 59% at P1. The study concluded that non sexed semen increased better gestation for Ongole crossbred cattle than sexed semen The study suggested that sexed semen remains able to be inseminated to those cattle with high attention on the sexing procedures. Keywords: Sexing, egg white sedimentation methods, NRR, S/C, CR

Penelitian ini dilakukan pada amoniasi jerami padi dan jerami padi fermentasi, dengan tujuan untuk meneloti kualitas dan kecernaan in vitro. Beberapa kendala pemanfaatan jerami padi sebagai pakan ternak adalah kualitas yang sangat rendah dengan kandungan serat yang tinggi, kadar protein dan daya cerna rendah. Rancangan yang digunakan Rancangan Acak kelompok, dengan 4 perlakuan yang digunakan jerami padi (kontrol), amoniasi jerami padi, dan jerami fermentasi I dan jerami fermentasi II. Bahan Kering dan Bahan Organik dicerna amoniasi jerami padi dan fermentasi jerami padi cenderung naik. Hal ini disebabkan adanya mikroorganisme probiotik dan penambahan molase pada fermentasi jerami merupakan sumber karbohidrat yang membantu dalam perbaikan tingkat ketersediaan nutrisi jerami. Kata Kunci: sumberdaya pakan local, integrasi ternak dan usahatani

The purpose of the study was to determine the correlation between cow weight and birth weight of Brahman Cross calves with different sex. The materials of the study were one hundred Brahman Cross cows which had been purposively selected. The variables of the study were cow body weight, calves birth weight, and sex. Data were analyzed using Pearson Correlation test (Product-Moment), Linear Regression and t Test. The study found that the birth weight diferred significantly (P

The purpose of this study was to determine the performance of reproduction in dairy cattle, the views of service per conception, days open, and calving interval. The material used in this study is the implementation of recording data or artificial insemination in 100 dairy cows females have at least twice to parturition known calving interval. Samples were selected randomly within the same district in Batu. The method used in this research is a case study. The data is taken primary and secondary data. Primary data retrieval is done by direct observation (observation), which includes several variables, where the variables include: the identity of the breeder, the number of cattle, cages and feeding and drinking. As for the secondary data obtained by recording the card Artificial Insemination (AI) owned by farmers. Sampling was done by purposive sampling and random with the following conditions: sampling was done by purposive sampling with the following conditions: minimal yearling heifer parturition twice and had a complete recording AI, including, the date AI and the date of parturition. Results from this study is the performance of reproduction in the dairy cooperatives Batu showed unfavorable results, average - the average total value of S/C of parity 1 s / d 6 is, 1.93 ± 1.18. As for the average - the average total value of DO and CI parity 1 s / d 5 is, 171.70 ± 132.20 days and 450.92 ± 132.83 days. In addition, the assessment of S / C, DO and CI at different parity using statistical tests showed that the S / C, DO and CI at various parity was not significantly different (P> 0.05).It concluded that reproduction display AI results in the KUD BATU less than the maximum and the difference between parity does not affect the value of the S / C, DO, and CI.

Effect of thawing technique to quality frozen semen spermatozoa in bali, madura and PO cattle ABSTRACT. The experiment was conducted from November 2011 to January 2012 in BBIB Singosari. The study aims was to determine the effect of thawing techniques on the quality frozen bovine sperm Bali, Madura and PO cattle. This material used 90 frozen straw of three cattle from the three breed. The method is an experiment with three treatments and 10 replications for frozen to thawing in temperature water is 5 0C of duration 60 seconds, 15 0C duration 30 seconds and 37 0C duration 15 seconds. Variables observed in this study were sperm quality are motility, viability and abnormality. Data in percentage transformed in to arcus sinus were analyzed using Completely Randomized Block Design (CRBD) with replication as blocks of 10x. The results showed the thawing at 37 0C duration 15 seconds to highly significant effect (P0,01) viability and motility and significant effect (P0.05). The abnormalities, no significant effect (P0,05). Breed cattle not significant effect to quality. It can be concluded that thawing techniques impact the motility and viability in frozen bovine semen spermatozoa Bali, Madura and PO cattle. Thawing using 37 0C temperature water with duration of 15 seconds is the best method to obtain the quality of spermatozoa post thawing frozen sperm.

The use of appropriate diluent is intended to ensure the physical and chemical requirements ofspermatozoa so that the quality, particularly capacitation can be maintained. This study aimed todetermine the quality of Limousin cattle sperm after sexing with density percoll centrifugation methodusing Andromed diluent and Cauda epididymal Plasma 2 (CEP-2) plus 10% egg yolk. Observations ofspermatozoa quality including : motility, viability, percentage of abnormal, concentrations, membraneintegrity and state of capacitation. The semen used were obtained from Limousin cattle at the Center forArtificial Insemination(BBIB) Singosari Malang, with motility criteria e” 70%. Data were analysed usinga paired T-test in order to determine differences in the use of thinning treatments at each layer. Theresults showed that both diluent, andromed and CEP-2 diluent plus 10% egg yolk were able to maintainthe quality of spermatozoa either in the upper and lower layers

The aim of this study was to observe the best extender in protecting the membrane of bovine spermatozoafollowing sexing by percoll density gradient centrifugation. Freshly collected semen were obtained fromBalai Besar Inseminasi Buatan Singosari-Malang. The semen were diluted in andromed and CaudalEpididymal Plasma-2 (CEP-2) added with 10% egg yolk extenders.The sperm membrane integrity wasobserved using Hypo-osmotic Swelling Test (HOST). Sperm capacitation and acrososome reaction wereassessed using Chlortetracycline Fluorescence Assay.The results showed that andromed and CEP-2 addedwith 10% egg yolk were able to retain the sperm membrane integrity, whereas sperm capacitation andacrosome reaction were kept low. Caudal Epididymal Plasma-2 (CEP-2) added with 10% egg yolk seemedto give better protection towards the sperm membrane intact in comparison to andromed extender.

The objective of this study was to determine sperm membrane stability after sexing with gradientalbumin (egg white) using andromed and CEP-2 supplemented with 10% egg yolk. The observations ofsperm membrane included membrane integrity, uncapacitated, capacitated and acrosome reacted. Albumingradient was made by mixing egg white with andromed or CEP-2 supplemented with 10% egg yolk resultingin a concentration of egg whites 10%, 30% and 50%. Membrane integrity was observed using HOST(Hypoosmotic Swelling Test), while uncapacitated, capacitated and acrosome reacted sperm were observedusing CTC (Chlortetracycline) staining. The results showed that both andromed and CEP-2 supplementedwith 10% egg yolk could protect membrane integrity, uncapacitated, capacitated and acrosome reactedsperm. Andromed and CEP-2 supplement with 10% egg yolk could reduce the demage of membrane integrityand uncapacitated sperm, whereas capacitated and acrosome reacted sperm could be kept low.

This study aimed to determine the effect of different methods of saving the storage by using a waterjacket and a free water jacket to quality semen Limousin cattle after being stored at a temperature of 5°C.Semen used the criteria motility e” + + collected two times a week. Semen evaluated macroscopically andmicroscopically and diluted in the ratio 1: 1 using the Cauda Epididymal Plasma (CEP-2) diluent added10% egg yolk. Stored in the refrigerator temperature 5°C and semen quality was observed immediatelyafter dilution, 1 hour, 2 hours, 3 hours, 24 hours, 48 hours and 72 hours. The results showed that themethod of free storage waterjacket better in maintaining the percentage of motility, viability, abnormalityand plasma membrane intact spermatozoa

Penelitian ini bertujuan mengetahui kualitas spermatozoa setelah sexing dengan gradien albumin (putih telur) menggunakan pengencer andromed dan cauda epididymal plasma-2 (CEP-2) ditambah kuning telur 10%. Pengamatan kualitas spermatozoa meliputi motilitas, viabilitas, abnormalitas, konsentrasi, dan total spermatozoa motil. Gradien albumin dibuat dengan cara mencampur putih telur dengan pengencer andromed atau CEP-2 ditambah kuning telur 10% sehingga menghasilkan konsentrasi putih telur 10, 30, dan 50%. Pengencer andromed menghasilkan motilitas spermatozoa hasil sexing pada lapisan atas yang lebih tinggi daripada pengencer CEP-2 ditambah kuning telur 10%. Pengencer andromed menghasilkan viabilitas, abnormalitas, konsentrasi, dan total spermatozoa motil yang sama seperti pengencer CEP-2 ditambah kuning telur 10%. Pengencer andromed dan CEP-2 ditambah kuning telur 10% dapat mengurangi penurunan kualitas spermatozoa.

Penelitian ini bertujuan mengetahui prospek sari buah tin lokal (Ficus glumerata Rob) di dalam pengencer berbasis Tris dan kuning telur untuk mempertahankan motilitas progresif spermatozoa kambing peranakan Boer yang disimpan pada suhu 4-5 C. Perlakuan yang diberikan adalah perbedaan dosis sari buah tin dalam masing-masing pengencer yakni 0, 1, 2, 3, 4, 5, 6, dan 7% (S0, S1, S2, S3, S4, S5, S6, dan S7). Pengamatan motilitas progresif spermatozoa dilakukan segera setelah pengenceran dan setiap 24 jam sampai jam ke-144 setelah pengeceran dan penyimpanan. Persentase motilitas progresifs permatozoa pada kelompok S4 (39,0±2,33) dan S6 (38,0±2,49) lebih tinggi dibandingkan S0; S1; S2; S3; dan S5 yakni masing-masing 32,0±1,86; 30,0±2,11; 29,0±1,80; 31,5±3,66; dan 34,5±3,02. Persentase motilitas terendah ditunjukkan oleh S7 yaitu 22,0±3,59. Dari hasil penelitian ini dapat disimpulkan bahwa sari buah tin mampu mempertahankan motilitas progresif spermatozoa kambing dan konsentrasi optimal dicapai pada 4 dan 6%.

Tujuan dari penelitian ini adalah mengetahui pengaruh penambahan kuning telur dalam pengencer CEP-2 terhadap motilitas dan viabilitas spermatozoa sapi Limousin. Penelitian menggunakan rancangan acak kelompok dengan 10 kali ulangan dari 10 ejakulat dari sapi yang berbeda. Spermatozoa disimpan selama delapan hari. Motilitas spermatozoa diamati dengan mikroskop cahaya (200x) pada suhu 37 C. Viabiltas spermatozoa diamati dengan pewarnaan eosin-negrosin menggunakan mikroskop cahaya (400x). Hasil penelitian menunjukkan suplementasi kuning telur pada pengencer CEP-2 berpengaruh terhadap motilitas dan viabilitas spermatozoa sapi Limousin selama penyimpanan pada suhu 4-5° C. Konsentrasi kuning telur terbaik adalah 20% dalam mempertahankan motilitas (44,25±3,92%) dan viabilitas (87,46±5,40%) spermatozoa sapi Limousin setelah penyimpanan delapan hari pada suhu 4-5° C.