51. A global benchmark study using affinity-based biosensors
- Author
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Aykut Üren, Stephen G. Brohawn, Eva Muñoz, Kenneth Miller, Mike Scott, Heather Hughes, Yasmina Noubia Abdiche, Rohn Lee Millican, Anne W Emerick, Jonathan Brooks, Michael B. Murphy, Charlene S. Lee, Carmelo Di Primo, Joshua Ballard, Quyhn Trinh, Giuseppe A. Papalia, Cynthia S. Hinck, Kevin Thompson, Peter Flynn, Joshua S. Klein, Martin A. Wear, Mark Toews, Richard N. Bohnsack, Mohammed Yousef, Daniel Malashock, Gaetano Barbato, Jill Raymond, Alison Joyce, Min Hsiang Yang, Julie Rothacker, Patrick England, Ashique Rafique, Jacinto López-Sagaseta, Scott L. Klakamp, Daulet Satpaev, Monica Dines, Danny Terwey, Islay Campbell, Joshua Thompson, Ben Busby, Tanmoy Ganguly, Gerardo Gutierrez-Sanchez, Sergei Shikov, Kevin Lindquist, Momchilo Vuyisich, Asya Grinberg, Krista Witte, Yun Hee Cho, Yue Ji Li, Hubert Mantz, Ewa Pol, Gonzalo Obal, Brian J. Pak, María J. Hernáiz, Peter Kainz, Erk Gedig, Mary M. Murphy, Tsafir Bravman, Janus Krarup, Gabrielle Zeder-Lutz, John Quinn, Thomas E. Ryan, Pietro Brandani, Dawn Kernaghan, Anca Clabbers, M. Brent Waddell, Agnes Puskas, Petr Skládal, Sanjay Nilapwar, Kara Herlihy, Gregor Anderluh, Federico Torta, Eric Hommema, Andrej Bavdek, Rejane Guimaraes, Anthony M. Giannetti, Eric Fang, Henrik Anderson, Anne Birgitte Bagge Hagel, Schuyler B Corry, Mark R. Witmer, Phillippe Neuner, Sébastien Wieckowski, Nico Dankbar, Alanna Pinkerton, Suparna Mundodo, Trevor D. Chapman, Sylvie Canepa, Satya P. Yadav, Rostislav Skrabana, Oleksandr Kalyuzhniy, Phini S Katsamba, Vidya Chandrasekaran, Eugene G. Chomey, Dana Reichmann, Katy McGirr, Dotzlaf Joe Edward, Mireille Baltzinger, Ite A. Laird-Offringa, Liann Wang, Erica Boni, Tiffany Tsang, Zaneta Nikolovska-Coleska, Andreas Schoenemann, Yuki Abe, Jamie Furneisen, Kenneth T. Lewis, Eileen M. Lafer, John Corbin, Satyen Gautam, Yuguo Feng, Olan Dolezal, Sylviane Hoos, James R. Horn, Bianca Beusink, Jinlin Peng, Otto Pritsch, Kristian H. Schlick, Ryan James Darling, Malgorzata Mikolajczyk, Quincy L. Carter, Jason T. Schuman, Yang Liu, Abdelkrim Khadir, Loïc Martin, Mark Alan Lewis, Ganeshram Krishnamoorthy, Andrew W. Drake, Jason Baardsnes, George Korza, Jesper Pass, Judie Berlier, Melicia Gainey, Nguyen Ly, James R. Partridge, Rosy Calvert, Roberta D'Agata, Rebecca L. Rich, Monica E. Ferreira, Phillip Page, Frank John Podlaski, Jay Duffner, Ruchira Das Gupta, Melanie Wong, Sergei Bibikov, Jiejin Li, Paola Torreri, Bruce A. Andrien, Peter Spies, Christina Boozer, David G. Myszka, University of Utah School of Medicine [Salt Lake City], KaloBios Pharmaceuticals [San Francisco], Schering–Plough Biopharma [Palo Alto], Nomadics [Oklahoma City], Biochemistry and Molecular Biophysics [Pasadena], California Institute of Technology (CALTECH), Department of Biochemistry and Molecular Biophysics, Columbia University [New York], Hartwell Center for Bioinformatics and Biotechnology [Memphis], St. Jude Children’s Research Hospital [Memphis], Universität Zürich [Zürich] = University of Zurich (UZH), Molecular Probes/Invitrogen [Eugene], Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), MerckKGaA, Merck & Co. Inc, Abbott Bioresearch Center [Worcester], Institut de biologie cellulaire et moléculaire [Strasbourg], Université de Strasbourg (UNISTRA), Reichert [Depew}, Momenta Pharmaceuticals [Cambridge], XOMA (U.S.), Department of Chemical and Biomolecular Engineering, National University of Singapore, National University of Singapore (NUS), Department of Biology [Ljubljiana], Biotechnical Faculty, University of Ljubljana, Department of Biological Chemistry (Weizmann Institute of Science), Weizmann Institute of Science [Rehovot, Israël], Molecular Biotechnology Core Laboratory [Cleveland], Cleveland Clinic Foundation, ThermoFisher Scientific [Rockford], Biacore/GE Healthcare [Uppsala], AstraZeneca [Hayward], Neurodegeneration Research Department [Harlow], GlaxoSmithKline [Harlow], MedImmune, Biacore/GE Healthcare [Piscataway], Department of Biochemistry [Milwaukee], Medical College of Wisconsin [Milwaukee] (MCW), Alexion Pharmaceuticals [Cheshire], Applied Biosystems [Foster City], diaDexus [South San Francisco], Eli Lilly and Company [Indianapolis], Center for Applied Medical Research [Plamplona] (CIMA), Universidad de Navarra [Pamplona] (UNAV), EMD Lexigen Research Center [Billerica], Department of Cell Biology and Neuroscience [Rome], Istituto Superiore di Sanita [Rome], Département de Biologie structurale et Chimie - Department of Structural Biology and Chemistry, Institut Pasteur [Paris], Department of Chemistry [Atlanta], Georgia State University, University System of Georgia (USG)-University System of Georgia (USG), Pfizer/Rinat Laboratories [South San Francisco], PDLBioPharma [Fremont], Department of Biochemistry [San Antonio], University of Texas Health Science Center at San Antonio [San Antonio], Akubio [Cambridge], ARN : régulations naturelle et artificielle, Université Bordeaux Segalen - Bordeaux 2-Institut Européen de Chimie et de Biologie-Institut National de la Santé et de la Recherche Médicale (INSERM), Wyeth Research [Cambridge], Department of Biochemistry and Molecular Biology [Odense], University of Southern Denmark (SDU), NovoNordisk [Gentofte], Södertörns University College [Huddinge], Department of Biochemistry [Philadelphia], Temple University [Philadelphia], Pennsylvania Commonwealth System of Higher Education (PCSHE)-Pennsylvania Commonwealth System of Higher Education (PCSHE), U.S. Food and Drug Administration (FDA), Department of Biochemical Engineering [London], University College of London [London] (UCL), Merck [Rome], Roche [Palo Alto], University of Twente [Netherlands], Agensys [Santa Monica], Novartis [Emeryville], Massachusetts Institute of Technology (MIT), Northern Illinois University, Institut Pasteur de Montevideo, Réseau International des Instituts Pasteur (RIIP), University of Manchester [Manchester], Department of Physiology [Baltimore], University of Maryland [Baltimore], Complex Carbohydrate Research Center [Athens, GA, USA], University of Georgia [USA], Adnexus Therapeutics [Waltham}, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), ForteBio [Menlo Park], University of Michigan [Ann Arbor], University of Michigan System, Human Genome Sciences [Rockville], Department of Chemical Sciences [Catania], University of Catania [Italy], Montana State University (MSU), GKT School of Biomedical Sciences [London], Organic and Pharmaceutical Chemistry Department [Madrid], Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Bio-Rad Haifa, Institute of Chemistry [Taipei], Academia Sinica, Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center at Houston (UTHealth), Fred Hutchinson Cancer Research Center [Seattle] (FHCRC), Division of Molecular Structure [London], National Institute of Medical Research (UK), Centre for Translational and Chemical Biology, Institute of Structural and Molecular Biology [Edinburgh], University of Edinburgh, Acceleron Pharma [Cambridge], Biotechnology Research Institute [Montreal], National Research Council of Canada (NRC), CSIRO Health Sciences and Nutrition [Parkville], Battelle Biomedical Research Center [Columbus], Attana AB [Stockholm], Corning [USA], School of Life Sciences, Institute for Chemistry and Bioanalytics [Muttenz], University of Applied Sciences Northwestern Switzerland (FHNW), Bio-Rad [Hercules], Monsanto [Chesterfield], Genzyme [Cambridge], Saarland University [Saarbrücken], Institute of Neuroimmunology of SAS [Bratislava], Bristol-Myers Squibb [Princeton], Array Biopharma [Boulder], Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO) (SIMoS), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Department of biochemistry, Masaryk University, Masaryk University [Brno] (MUNI), University of Connecticut Health Center [Farmington], University of Southern California, Los Angeles, GeminX Biotechnologies [Montreal], Hoffman–La Roche [Nutley], IRBM, Pomezia [Rome], Ludwig Institute for Cancer Research, Regeneron Pharmaceuticals [Tarrytown], University of Muenster, Department of Molecular Biology [Salzburg], University of Salzburg, XanTec Bioanalytics [Muenster], Biosciences Division [Los Alamos], Los Alamos National Laboratory (LANL), Lumera [Bothell], Arizona State University [Tempe] (ASU), Dyax [Cambridge], Lombardi Comprehensive Cancer Center, Georgetown University, Department of Biochemistry [Seattle], University of Washington [Seattle], ZymoGenetics, Bio-Rad Canada [Edmonton], Bio-Rad Canada [Toronto], St Jude Children's Research Hospital, University of Ljubljana, Istituto Superiore di Sanità (ISS), Institut Pasteur [Paris] (IP), University of Texas Health Science Center at San Antonio [San Antonio, Tx, USA], University of Twente, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), University of Southern California (USC), Georgetown University [Washington] (GU), Pasteur Tunis, Institut, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), University of Zürich [Zürich] (UZH), Weizmann Institute of Science, Medical College of Wisconsin [Milwaukee], Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Complutense University of Madrid (UCM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Masaryk University
- Subjects
Analyte ,Biophysics ,Optical biosensor ,Antibodies, Catalytic ,Biosensing Techniques ,Ligands ,01 natural sciences ,Biochemistry ,Article ,Biacore ,Glutathione transferase ,03 medical and health sciences ,Surface plasmon resonance ,Molecular Biology ,Kinetic rate constant ,030304 developmental biology ,Glutathione Transferase ,0303 health sciences ,[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology ,Chromatography ,Binding Sites ,Chemistry ,010401 analytical chemistry ,A protein ,Proteins ,Cell Biology ,0104 chemical sciences ,Benchmarking ,Kinetics ,Yield (chemistry) ,Benchmark (computing) ,IR-68798 ,Biosensor ,EWI-16945 ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
International audience; To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used. (C) 2008 Elsevier Inc. All rights reserved.
- Published
- 2009
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