Search

Your search keyword '"Thomas, R.L."' showing total 291 results
Start Over Author "Thomas, R.L."
291 results on '"Thomas, R.L."'

53. Residual pyruvate kinase activity in PKLR-deficient erythroid precursors of a patient suffering from severe haemolytic anaemia.

Author

Klei, Thomas R.L., Kheradmand Kia, Sima, Veldthuis, Martijn, Beuger, Boukje M., Geissler, Judy, Dehbozorgian, Javad, Karimi, Mehran, Bruggen, Robin, and Zwieten, Rob

Subjects
*PYRUVATE kinase, *HEMOLYTIC anemia, *ERYTHROCYTES, *RETICULOCYTES, *GENETIC mutation, *GENETIC code, *GLYCOLYSIS, *PATIENTS
Abstract

Objective Here, we present a 7-year-old patient suffering from severe haemolytic anaemia. The most common cause of chronic hereditary non-spherocytic haemolytic anaemia is red blood cell pyruvate kinase (PK-R) deficiency. Because red blood cells rely solely on glycolysis to generate ATP, PK-R deficiency can severely impact energy supply and cause reduction in red blood cell lifespan. We determined the underlying cause of the anaemia and investigated how erythroid precursors in the patient survive. Methods PK activity assays, Western blot and Sanger sequencing were employed to determine the underlying cause of the anaemia. Patient erythroblasts were cultured and reticulocytes were isolated to determine PK-R and PKM2 contribution to glycolytic activity during erythrocyte development. Results We found a novel homozygous mutation (c.583G>A) in the PK-R coding gene ( PKLR). Although this mutation did not influence PKLR mRNA production, no PK-R protein could be detected in the red blood cells nor in its precursors. In spite of the absence of PK-R, the reticulocytes of the patient exhibited 20% PK activity compared with control. Western blotting revealed that patient erythroid precursors, like controls, express residual PKM2. Conclusions We conclude that PKM2 rescues glycolysis in PK-R-deficient erythroid precursors. [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

55. Time Resolved Resonance Raman Study of S1 cis-Stilbene and its Deuterated Isotopomers

Author

Kwok, W.M., Ma, C., Phillips, D., Beeby, A., Marder, T.B., Thomas, R.L., Tschuschke, C., Baranović, Goran, Matousek, Pavel, Towrie, M., and Parker, A.W.

Subjects
cis-stilbene, Raman spectroscopy, excited state
Abstract

We present the picosecond time-resolved resonance Raman (ps-TR3) spectra of the first excited singlet state (S1) of cis-stilbene (d0), fully deuterated cis-stilbene (d12) and  - ’ -d2-cis-stilbene (d2) measured in n-hexane at pump and probe wavelengths 267 and 630 nm, respectively. The main observation is that in the lower wavenumber region the spectra are dominated by a 2nd order overtone progression originating from a 229 cm-1 fundamental band for the S1 d0 cis-stilbene. The observed low-wavenumber modes of the excited cis-stilbene are substantially more resonantly enhanced than the modes around 1600 cm-1.

Published
2003

60. Pediatrics: A meta-analysis of the effects of oral zinc in the treatment of acute and persistent diarrhea

Author

Lukacik, M., Thomas, R.L., and Aranda, J.V.

Subjects
Diagnosis, Care and treatment, Usage, Risk factors, Dosage and administration, Methods, Zinc (Nutrient) -- Dosage and administration -- Usage, Meta-analysis -- Usage -- Methods, Childhood diarrhea -- Risk factors -- Diagnosis -- Care and treatment, Diarrhea in children -- Risk factors -- Diagnosis -- Care and treatment, Zinc in the body -- Dosage and administration -- Usage
Abstract

2008;121:326-336. OBJECTIVE: Children in developing countries are at a high risk for zinc deficiency. Supplemental zinc has previously been shown to provide therapeutic benefits in diarrhea. The objective of this [...]

Published
2008

61. IMMUNOEXPRESSION OF ZEB1 AND SIP1 IN HUMAN BLADDER CANCER

Author

Pal, Raj P, primary, Ruddick, Andrew, additional, Qazi, Hassan A.R, additional, Mayer, Nick J, additional, Sayan, Emre, additional, Yagci, Tamer, additional, Kriajevska, Marina, additional, Tulchinsky, Eugene, additional, Griffiths, Thomas R.L, additional, and Mellon, John K, additional

Published
2009
Full Text
View/download PDF

65. Dynamic three-dimensional spiral computed tomographic cysto-urethrography: a novel technique for evaluating post-traumatic posterior urethral defects

Author

KAMAT, NAGESH, primary, DASGUPTA, RANAN, additional, SHABBIR, MAJID, additional, NEULANDER, ENDR Z., additional, KLEIN, JOSEPH, additional, KANETI, JACOB, additional, JOSEPH, JEAN V., additional, PATEL, HITENDRA R.H., additional, MCHUGH, LYNSEY A., additional, GRIFFITHS, THOMAS R.L., additional, SHERGILL, IQBAL S., additional, HAMID, RIZWAN, additional, GUPTA, SUJOY, additional, MAMMEN, KIM J., additional, WALTON, THOMAS J., additional, and MICELI, PAULA, additional

Published
2008
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results