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574 results on '"Thissen, Helmut"'

51. Advancing of 3D-Printed Titanium Implants with Combined Antibacterial Protection Using Ultrasharp Nanostructured Surface and Gallium-Releasing Agents

Author

Maher, Shaheer, primary, Linklater, Denver, additional, Rastin, Hadi, additional, Liao, Sandy Tzu-Ying, additional, Martins de Sousa, Karolinne, additional, Lima-Marques, Luis, additional, Kingshott, Peter, additional, Thissen, Helmut, additional, Ivanova, Elena P., additional, and Losic, Dusan, additional

Published
2021
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60. Combined immunocapture and laser desorption/ ionization mass spectrometry on porous silicon

Author

Lowe, Rachel D., Szili, Endre J., Kirkbride, Paul, Thissen, Helmut, Siuzdak, Gary, and Voelcker, Nicolas H.

Subjects
Silicon -- Chemical properties, Silicon -- Testing, Mass spectrometry -- Methods, Mass spectrometry -- Technology application, Materials -- Testing, Materials -- Methods, Materials -- Technology application, Technology application, Chemistry
Abstract

There is considerable interest in the highly parallelized mass spectrometry analysis of complex sample mixtures without any time-consuming prepurification. Porous silicon-based laser deserption/ionization mass spectrometry (pSi LDI-MS) is enabling technology for such analysis. Previous studies have focused on pSi surface functionalization to enhance sensitivity of detection and engineer surfaces for sample capture and enrichment in LDI-MS analysis. In this report, we build on this work by showing that surface functionalization of thin pSi films can be extended to the covalent immobilization of antibodies, producing a porous immunoaffinity surface. We demonstrate highly selective mass spectrometric detection of illicit drugs (benzodiazepines) on pSi films displaying antibenzodiazepine antibodies covalently immobilized via isocyanate chemistry. The effects of antibody immobilization conditions, antibody concentration, and surface blocking on LDI-MS performance and selectivity were studied. X-ray photoelectron spectroscopy (XPS) was instrumental in characterizing surface chemistry and optimizing LDI-MS performance. Overall, our approach is suitable for rapid and sensitive confirmatory analysis in forensic toxicology requiring only minimal sample volume and may be applied to other areas requiring small molecular analysis such as metabolomics and pharmscology. 10.1021/ac100455x

Published
2010

61. Time-of-flight-secondary ion mass spectrometry study of the temperature dependence of protein adsorption onto Poly(N-isopropylacrylamide) graft coatings

Author

Cole, Martin A., Jasienlak, Marek, Thissen, Helmut, Voelcker, Nicolas H., and Griesser, Hans J.

Subjects
Time-of-flight mass spectrometry -- Methods, Protein research -- Methods, Adsorption -- Research, Chemistry
Abstract

Stimuli-responsive materials show considerable promise for applications that require control over biomolecule interactions at solid material interfaces. Graft coatings of poly(N-isopropylacrylamide) (pNIPAM) are of interest for biomedical and biotechnological applications due to their temperature-dependent switching of surface properties between adhesive and nonadhesive slates for cells and proteins. The characterization of protein adsorption to these switchable coatings is a formidable task since switching not only influences file affinity for proteins but at the same time induces a significant change in the coating. Here, the highly sensitive analytical technique of llme-of-flight-secondary ion mass spectrometry (TOFSIMS) combined with principal component analysis (PCA) was used for the characterization of protein adsorption onto pNIPAM coatings prepared by free radical polymerization onto-bound polymerlzable groups. Adsorption of bovine serum albumin and lysozyme onto pNIPAM coatings from phosphate buffered solutions was investigated at temperatures above and below the polymer's lower critical solution temperature (LCST). Below the LCST, no adsorbed proteins could be detected even with this ultrasensitive method. Whereas above the LCST, adsorbed protein was detected in amounts corresponding at less than the monolayer. PCA loadings plots showed that adventitious contaminants, which might lead to confounding or misleading spectral changes upon protein exposure, were not observed.

Published
2009

63. Harnessing Colloidal Self-Assembled Patterns (cSAPs) to Regulate Bacterial and Human Stem Cell Response at Biointerfaces In Vitro and In Vivo

Author

Shi, Yue, primary, Lin, Jiao, additional, Tao, Xuelian, additional, Qu, Jing, additional, Liao, Shumin, additional, Li, Mengyao, additional, Deng, Ke, additional, Du, Ping, additional, Liu, Kun, additional, Thissen, Helmut, additional, Li, Liang, additional, Kingshott, Peter, additional, and Wang, Peng-Yuan, additional

Published
2021
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64. Next Generation Cell Culture Tools Featuring Micro‐ and Nanotopographies for Biological Screening

Author

Carthew, James, primary, Abdelmaksoud, Hazem H., additional, Cowley, Karla J., additional, Hodgson‐Garms, Margeaux, additional, Elnathan, Roey, additional, Spatz, Joachim P., additional, Brugger, Juergen, additional, Thissen, Helmut, additional, Simpson, Kaylene J., additional, Voelcker, Nicolas H., additional, Frith, Jessica E., additional, and Cadarso, Victor J., additional

Published
2021
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66. Precision Surface Microtopography Regulates Cell Fate via Changes to Actomyosin Contractility and Nuclear Architecture

Author

Carthew, James, primary, Abdelmaksoud, Hazem H., additional, Hodgson‐Garms, Margeaux, additional, Aslanoglou, Stella, additional, Ghavamian, Sara, additional, Elnathan, Roey, additional, Spatz, Joachim P., additional, Brugger, Juergen, additional, Thissen, Helmut, additional, Voelcker, Nicolas H., additional, Cadarso, Victor J., additional, and Frith, Jessica E., additional

Published
2021
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77. In Situ Surface Modification of Microfluidic Blood–Brain-Barriers for Improved Screening of Small Molecules and Nanoparticles

Author

Peng, Bo, primary, Tong, Ziqiu, additional, Tong, Wing Yin, additional, Pasic, Paul J., additional, Oddo, Arianna, additional, Dai, Yitian, additional, Luo, Meihua, additional, Frescene, Juliette, additional, Welch, Nicholas G., additional, Easton, Christopher D., additional, Thissen, Helmut, additional, and Voelcker, Nicolas H., additional

Published
2020
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81. Hyperosmotic Infusion and Oxidized Surfaces Are Essential for Biofilm Formation of Staphylococcus capitis From the Neonatal Intensive Care Unit

Author

Qu, Yue, primary, Li, Yali, additional, Cameron, David R., additional, Easton, Christopher D., additional, Zhu, Xuebo, additional, Zhu, Minli, additional, Salwiczek, Mario, additional, Muir, Benjamin W., additional, Thissen, Helmut, additional, Daley, Andrew, additional, Forsythe, John S., additional, Peleg, Anton Y., additional, and Lithgow, Trevor, additional

Published
2020
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88. New prebiotic chemistry inspired filter media for stormwater/greywater disinfection

Author

Jung, J., Menzies, D. J., Thissen, Helmut, Easton, C. D., Evans, R. A., Henry, R., Deletic, A., McCarthy, D. T., Jung, J., Menzies, D. J., Thissen, Helmut, Easton, C. D., Evans, R. A., Henry, R., Deletic, A., and McCarthy, D. T.

Abstract

Greywater and stormwater have received significant attention due to increasing water scarcity. Passive filtration such as biofiltration has been a popular treatment method with its low energy input and environmental friendliness. However, pathogen removal capacity needs improvement to achieve safe water quality. In this study, a prebiotic chemistry inspired copolymer based on aminomalononitrile and 3,4,5-trihydroxybenzaldehyde (AMNT30) was introduced to develop antimicrobial media for passive filtration. The AMNT30 polymer provided an adhesive coating on zeolite substrates following a spontaneous polymerisation process at room temperature. AMNT30 coated media were investigated for metal loading capacity, surface morphology, E. coli removal and metal leaching after filtration of different water sources (i.e. stormwater, greywater, and deionised water) at low/high conductivity. The coating enhanced metal ion loading on the surface and demonstrated that >8 log reduction of E. coli can be achieved for silver loaded materials compared to a 1 log reduction for copper loaded materials. The coating also increased the stability of the metals on the media irrespective of inflow characteristics. This study provided the first example using AMNT30 to create antimicrobial water purification media. It is expected that this technology will find applications in the water treatment industry.

Published
2019

89. The fate of osteoblast-like MG-63 cells on pre-infected bactericidal nanostructured titanium surfaces

Author

Wandiyanto, Jason V., Truong, Vi Khanh, Kobaisi, Mohammad Al, Juodkazis, Saulius, Thissen, Helmut, Bazaka, Olha, Bazaka, Kateryna, Crawford, Russell J., Ivanova, Elena P., Wandiyanto, Jason V., Truong, Vi Khanh, Kobaisi, Mohammad Al, Juodkazis, Saulius, Thissen, Helmut, Bazaka, Olha, Bazaka, Kateryna, Crawford, Russell J., and Ivanova, Elena P.

Abstract

Biomaterials that have been newly implanted inside the body are the substratum targets for a "race for the surface", in which bacterial cells compete against eukaryotic cells for the opportunity to colonize the surface. A victory by the former often results in biomaterial-associated infections, which can be a serious threat to patient health and can undermine the function and performance of the implant. Moreover, bacteria can often have a 'head start' if implant contamination has taken place either prior to or during the surgery. Current prevention and treatment strategies often rely on systemic antibiotic therapies, which are becoming increasingly ineffective due to a growing prevalence of antibiotic-resistant bacteria. Nanostructured surfaces that kill bacteria by physically rupturing bacterial cells upon contact have recently emerged as a promising solution for the mitigation of bacterial colonization of implants. Furthermore, these nanoscale features have been shown to enhance the adhesion and proliferation of eukaryotic cells, which is a key to, for example, the successful osseointegration of load-bearing titanium implants. The bactericidal activity and biocompatibility of such nanostructured surfaces are often, however, examined separately, and it is not clear to what extent bacterial cell-surface interactions would affect the subsequent outcomes of host-cell attachment and osseointegration processes. In this study, we investigated the ability of bactericidal nanostructured titanium surfaces to support the attachment and growth of osteoblast-like MG-63 human osteosarcoma cells, despite them having been pre-infected with pathogenic bacteria. MG-63 is a commonly used osteoblastic model to study bone cell viability, adhesion, and proliferation on the surfaces of load-bearing biomaterials, such as titanium. The nanostructured titanium surfaces used here were observed to kill the pathogenic bacteria, whilst simultaneously enhancing the growth of MG-63 cells in v

Published
2019

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