Search

Your search keyword '"Tassi, Renata A."' showing total 63 results
Start Over Author "Tassi, Renata A."
63 results on '"Tassi, Renata A."'

51. Additional file 5: of FOXM1 expression is significantly associated with chemotherapy resistance and adverse prognosis in non-serous epithelial ovarian cancer patients

Author

Tassi, Renata, Todeschini, Paola, Siegel, Eric, Calza, Stefano, Cappella, Paolo, Ardighieri, Laura, Cadei, Moris, Bugatti, Mattia, Romani, Chiara, Bandiera, Elisabetta, Zanotti, Laura, Tassone, Laura, Guarino, Donatella, Santonocito, Concetta, Capoluongo, Ettore, Beltrame, Luca, Erba, Eugenio, Marchini, Sergio, D’Incalci, Maurizio, Donzelli, Carla, Santin, Alessandro, Pecorelli, Sergio, Sartori, Enrico, Bignotti, Eliana, Odicino, Franco, and Ravaggi, Antonella more...

Subjects
endocrine system diseases, female genital diseases and pregnancy complications, 3. Good health
Abstract

Results of the characterization of OSPC2 and EOC-CC1 cell lines; Table S6: Panel of immunocytochemical stains in EOC cell cultures; Table S7: STR profiles of EOC-CC1 and OSPC2 biopsies and derived cell lines; Table S8: List of BRCA1 and BRCA2 sequence variants in OSPC2 cell line; Table S9: List of BRCA1 and BRCA2 sequence variants in EOC-CC1 cell line; Figure S1: Immunohistochemical stain for FOXM1 in original clinical tumor samples and in derived cell lines. Figure S2: EOC cell lines growth curves; Table S10: Optimal cell densities for seeding different cell lines in culture. (DOCX 552Â kb) more...

52. Additional file 5: of FOXM1 expression is significantly associated with chemotherapy resistance and adverse prognosis in non-serous epithelial ovarian cancer patients

Author

Tassi, Renata, Todeschini, Paola, Siegel, Eric, Calza, Stefano, Cappella, Paolo, Ardighieri, Laura, Cadei, Moris, Bugatti, Mattia, Romani, Chiara, Bandiera, Elisabetta, Zanotti, Laura, Tassone, Laura, Guarino, Donatella, Santonocito, Concetta, Capoluongo, Ettore, Beltrame, Luca, Erba, Eugenio, Marchini, Sergio, D’Incalci, Maurizio, Donzelli, Carla, Santin, Alessandro, Pecorelli, Sergio, Sartori, Enrico, Bignotti, Eliana, Odicino, Franco, and Ravaggi, Antonella more...

Subjects
endocrine system diseases, female genital diseases and pregnancy complications, 3. Good health
Abstract

Results of the characterization of OSPC2 and EOC-CC1 cell lines; Table S6: Panel of immunocytochemical stains in EOC cell cultures; Table S7: STR profiles of EOC-CC1 and OSPC2 biopsies and derived cell lines; Table S8: List of BRCA1 and BRCA2 sequence variants in OSPC2 cell line; Table S9: List of BRCA1 and BRCA2 sequence variants in EOC-CC1 cell line; Figure S1: Immunohistochemical stain for FOXM1 in original clinical tumor samples and in derived cell lines. Figure S2: EOC cell lines growth curves; Table S10: Optimal cell densities for seeding different cell lines in culture. (DOCX 552Â kb) more...

53. Additional file 11: Figure S7. of FOXM1 expression is significantly associated with chemotherapy resistance and adverse prognosis in non-serous epithelial ovarian cancer patients

Author

Tassi, Renata, Todeschini, Paola, Siegel, Eric, Calza, Stefano, Cappella, Paolo, Ardighieri, Laura, Cadei, Moris, Bugatti, Mattia, Romani, Chiara, Bandiera, Elisabetta, Zanotti, Laura, Tassone, Laura, Guarino, Donatella, Santonocito, Concetta, Capoluongo, Ettore, Beltrame, Luca, Erba, Eugenio, Marchini, Sergio, D’Incalci, Maurizio, Donzelli, Carla, Santin, Alessandro, Pecorelli, Sergio, Sartori, Enrico, Bignotti, Eliana, Odicino, Franco, and Ravaggi, Antonella more...

Subjects
endocrine system diseases, female genital diseases and pregnancy complications, 3. Good health
Abstract

Functional analysis of the genome-wide transcriptional response in FOXM1-silenced EOC cells. Putative network reconstruction on EOC-CC1 and OSPC2 cells. (PDF 178Â kb)

54. Additional file 2: of FOXM1 expression is significantly associated with chemotherapy resistance and adverse prognosis in non-serous epithelial ovarian cancer patients

Author

Tassi, Renata, Todeschini, Paola, Siegel, Eric, Calza, Stefano, Cappella, Paolo, Ardighieri, Laura, Cadei, Moris, Bugatti, Mattia, Romani, Chiara, Bandiera, Elisabetta, Zanotti, Laura, Tassone, Laura, Guarino, Donatella, Santonocito, Concetta, Capoluongo, Ettore, Beltrame, Luca, Erba, Eugenio, Marchini, Sergio, D’Incalci, Maurizio, Donzelli, Carla, Santin, Alessandro, Pecorelli, Sergio, Sartori, Enrico, Bignotti, Eliana, Odicino, Franco, and Ravaggi, Antonella more...

Subjects
3. Good health
Abstract

Description of microarray analysis on EOC tissue specimens and cell lines. Table S3: Genes related to tumor invasion-metastasis used for the Gene-Sets Enrichment Analysis (GSEA). (DOCX 18Â kb)

55. Additional file 6: Figure S3. of FOXM1 expression is significantly associated with chemotherapy resistance and adverse prognosis in non-serous epithelial ovarian cancer patients

Author

Tassi, Renata, Todeschini, Paola, Siegel, Eric, Calza, Stefano, Cappella, Paolo, Ardighieri, Laura, Cadei, Moris, Bugatti, Mattia, Romani, Chiara, Bandiera, Elisabetta, Zanotti, Laura, Tassone, Laura, Guarino, Donatella, Santonocito, Concetta, Capoluongo, Ettore, Beltrame, Luca, Erba, Eugenio, Marchini, Sergio, D’Incalci, Maurizio, Donzelli, Carla, Santin, Alessandro, Pecorelli, Sergio, Sartori, Enrico, Bignotti, Eliana, Odicino, Franco, and Ravaggi, Antonella more...

Subjects
endocrine system diseases, female genital diseases and pregnancy complications, 3. Good health
Abstract

Flow cytometric and BrdU analyses of DNA content in siFOXM1 EOC cells. (DOCX 1100Â kb)

56. Additional file 1: of FOXM1 expression is significantly associated with chemotherapy resistance and adverse prognosis in non-serous epithelial ovarian cancer patients

Author

Tassi, Renata, Todeschini, Paola, Siegel, Eric, Calza, Stefano, Cappella, Paolo, Ardighieri, Laura, Cadei, Moris, Bugatti, Mattia, Romani, Chiara, Bandiera, Elisabetta, Zanotti, Laura, Tassone, Laura, Guarino, Donatella, Santonocito, Concetta, Capoluongo, Ettore, Beltrame, Luca, Erba, Eugenio, Marchini, Sergio, D’Incalci, Maurizio, Donzelli, Carla, Santin, Alessandro, Pecorelli, Sergio, Sartori, Enrico, Bignotti, Eliana, Odicino, Franco, and Ravaggi, Antonella more...

Subjects
3. Good health
Abstract

Detailed protocols for the establishment and the characterization of the EOC cell lines. Table S1: Clinical features of patients and tumor characteristics of samples used to derive cell lines; Table S2: Antibodies features and detailed staining protocols; description of short tandem repeat (STR) DNA profiling; description of BRCA1/2 sequencing; determination of cell linesâ growth rate. (DOCX 23Â kb) more...

57. Additional file 2: of FOXM1 expression is significantly associated with chemotherapy resistance and adverse prognosis in non-serous epithelial ovarian cancer patients

Author

Tassi, Renata, Todeschini, Paola, Siegel, Eric, Calza, Stefano, Cappella, Paolo, Ardighieri, Laura, Cadei, Moris, Bugatti, Mattia, Romani, Chiara, Bandiera, Elisabetta, Zanotti, Laura, Tassone, Laura, Guarino, Donatella, Santonocito, Concetta, Capoluongo, Ettore, Beltrame, Luca, Erba, Eugenio, Marchini, Sergio, D’Incalci, Maurizio, Donzelli, Carla, Santin, Alessandro, Pecorelli, Sergio, Sartori, Enrico, Bignotti, Eliana, Odicino, Franco, and Ravaggi, Antonella more...

Subjects
3. Good health
Abstract

Description of microarray analysis on EOC tissue specimens and cell lines. Table S3: Genes related to tumor invasion-metastasis used for the Gene-Sets Enrichment Analysis (GSEA). (DOCX 18Â kb)

58. Additional file 1: of FOXM1 expression is significantly associated with chemotherapy resistance and adverse prognosis in non-serous epithelial ovarian cancer patients

Author

Tassi, Renata, Todeschini, Paola, Siegel, Eric, Calza, Stefano, Cappella, Paolo, Ardighieri, Laura, Cadei, Moris, Bugatti, Mattia, Romani, Chiara, Bandiera, Elisabetta, Zanotti, Laura, Tassone, Laura, Guarino, Donatella, Santonocito, Concetta, Capoluongo, Ettore, Beltrame, Luca, Erba, Eugenio, Marchini, Sergio, D’Incalci, Maurizio, Donzelli, Carla, Santin, Alessandro, Pecorelli, Sergio, Sartori, Enrico, Bignotti, Eliana, Odicino, Franco, and Ravaggi, Antonella more...

Subjects
3. Good health
Abstract

Detailed protocols for the establishment and the characterization of the EOC cell lines. Table S1: Clinical features of patients and tumor characteristics of samples used to derive cell lines; Table S2: Antibodies features and detailed staining protocols; description of short tandem repeat (STR) DNA profiling; description of BRCA1/2 sequencing; determination of cell linesâ growth rate. (DOCX 23Â kb) more...

59. Additional file 6: Figure S3. of FOXM1 expression is significantly associated with chemotherapy resistance and adverse prognosis in non-serous epithelial ovarian cancer patients

Author

Tassi, Renata, Todeschini, Paola, Siegel, Eric, Calza, Stefano, Cappella, Paolo, Ardighieri, Laura, Cadei, Moris, Bugatti, Mattia, Romani, Chiara, Bandiera, Elisabetta, Zanotti, Laura, Tassone, Laura, Guarino, Donatella, Santonocito, Concetta, Capoluongo, Ettore, Beltrame, Luca, Erba, Eugenio, Marchini, Sergio, D’Incalci, Maurizio, Donzelli, Carla, Santin, Alessandro, Pecorelli, Sergio, Sartori, Enrico, Bignotti, Eliana, Odicino, Franco, and Ravaggi, Antonella more...

Subjects
endocrine system diseases, female genital diseases and pregnancy complications, 3. Good health
Abstract

Flow cytometric and BrdU analyses of DNA content in siFOXM1 EOC cells. (DOCX 1136Â kb)

60. Additional file 6: Figure S3. of FOXM1 expression is significantly associated with chemotherapy resistance and adverse prognosis in non-serous epithelial ovarian cancer patients

Author

Tassi, Renata, Todeschini, Paola, Siegel, Eric, Calza, Stefano, Cappella, Paolo, Ardighieri, Laura, Cadei, Moris, Bugatti, Mattia, Romani, Chiara, Bandiera, Elisabetta, Zanotti, Laura, Tassone, Laura, Guarino, Donatella, Santonocito, Concetta, Capoluongo, Ettore, Beltrame, Luca, Erba, Eugenio, Marchini, Sergio, D’Incalci, Maurizio, Donzelli, Carla, Santin, Alessandro, Pecorelli, Sergio, Sartori, Enrico, Bignotti, Eliana, Odicino, Franco, and Ravaggi, Antonella more...

Subjects
endocrine system diseases, female genital diseases and pregnancy complications, 3. Good health
Abstract

Flow cytometric and BrdU analyses of DNA content in siFOXM1 EOC cells. (DOCX 1100Â kb)

61. Additional file 1: of FOXM1 expression is significantly associated with chemotherapy resistance and adverse prognosis in non-serous epithelial ovarian cancer patients

Author

Tassi, Renata, Todeschini, Paola, Siegel, Eric, Calza, Stefano, Cappella, Paolo, Ardighieri, Laura, Cadei, Moris, Bugatti, Mattia, Romani, Chiara, Bandiera, Elisabetta, Zanotti, Laura, Tassone, Laura, Guarino, Donatella, Santonocito, Concetta, Capoluongo, Ettore, Beltrame, Luca, Erba, Eugenio, Marchini, Sergio, D’Incalci, Maurizio, Donzelli, Carla, Santin, Alessandro, Pecorelli, Sergio, Sartori, Enrico, Bignotti, Eliana, Odicino, Franco, and Ravaggi, Antonella more...

Subjects
3. Good health
Abstract

Detailed protocols for the establishment and the characterization of the EOC cell lines. Table S1: Clinical features of patients and tumor characteristics of samples used to derive cell lines; Table S2: Antibodies features and detailed staining protocols; description of short tandem repeat (STR) DNA profiling; description of BRCA1/2 sequencing; determination of cell linesâ growth rate. (DOCX 23Â kb) more...

62. Additional file 2: of FOXM1 expression is significantly associated with chemotherapy resistance and adverse prognosis in non-serous epithelial ovarian cancer patients

Author

Tassi, Renata, Todeschini, Paola, Siegel, Eric, Calza, Stefano, Cappella, Paolo, Ardighieri, Laura, Cadei, Moris, Bugatti, Mattia, Romani, Chiara, Bandiera, Elisabetta, Zanotti, Laura, Tassone, Laura, Guarino, Donatella, Santonocito, Concetta, Capoluongo, Ettore, Beltrame, Luca, Erba, Eugenio, Marchini, Sergio, D’Incalci, Maurizio, Donzelli, Carla, Santin, Alessandro, Pecorelli, Sergio, Sartori, Enrico, Bignotti, Eliana, Odicino, Franco, and Ravaggi, Antonella more...

Subjects
3. Good health
Abstract

Description of microarray analysis on EOC tissue specimens and cell lines. Table S3: Genes related to tumor invasion-metastasis used for the Gene-Sets Enrichment Analysis (GSEA). (DOCX 18Â kb)

63. Identification of stably expressed reference small non-coding RNAs for microRNA quantification in high-grade serous ovarian carcinoma tissues.

Author

Bignotti E, Calza S, Tassi RA, Zanotti L, Bandiera E, Sartori E, Odicino FE, Ravaggi A, Todeschini P, and Romani C

Subjects
Adult, Aged, Aged, 80 and over, Case-Control Studies, Computer Simulation, Female, Gene Expression Profiling, Humans, MicroRNAs metabolism, Middle Aged, RNA, Small Untranslated metabolism, Reference Standards, Reproducibility of Results, Sequence Analysis, RNA, Software, Gene Expression Regulation, Neoplastic, MicroRNAs genetics, Neoplasms, Cystic, Mucinous, and Serous genetics, Ovarian Neoplasms genetics, RNA, Small Untranslated genetics, Real-Time Polymerase Chain Reaction methods, Real-Time Polymerase Chain Reaction standards
Abstract

MicroRNAs (miRNAs) belong to a family of small non-coding RNAs (sncRNAs) playing important roles in human carcinogenesis. Multiple investigations reported miRNAs aberrantly expressed in several cancers, including high-grade serous ovarian carcinoma (HGS-OvCa). Quantitative PCR is widely used in studies investigating miRNA expression and the identification of reliable endogenous controls is crucial for proper data normalization. In this study, we aimed to experimentally identify the most stable reference sncRNAs for normalization of miRNA qPCR expression data in HGS-OvCa. Eleven putative reference sncRNAs for normalization (U6, SNORD48, miR-92a-3p, let-7a-5p, SNORD61, SNORD72, SNORD68, miR-103a-3p, miR-423-3p, miR-191-5p, miR-16-5p) were analysed on a total of 75 HGS-OvCa and 30 normal tissues, using a highly specific qPCR. Both the normal tissues considered to initiate HGS-OvCa malignant transformation, namely ovary and fallopian tube epithelia, were included in our study. Stability of candidate endogenous controls was evaluated using an equivalence test and validated by geNorm and NormFinder algorithms. Combining results from the three different statistical approaches, SNORD48 emerged as stably and equivalently expressed between malignant and normal tissues. Among malignant samples, considering groups based on residual tumour, miR-191-5p was identified as the most equivalent sncRNA. On the basis of our results, we support the use of SNORD48 as best reference sncRNA for relative quantification in miRNA expression studies between HGS-OvCa and normal controls, including the first time both the normal tissues supposed to be HGS-OvCa progenitors. In addition, we recommend miR-191-5p as best reference sncRNA in miRNA expression studies with prognostic intent on HGS-OvCa tissues., (© 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.) more...

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results