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569 results on '"Takatsuto, Suguru"'

51. Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis (1)

Author

Shimada, Yukihisa, Fujioka, Shozo, Miyauchi, Narumasa, Kushiro, Masayo, Takatsuto, Suguru, Nomura, Takahito, Yokota, Takao, Kamiya, Yuyi, Bishop, Gerard J., and Yoshida, Shigeo

Subjects
Plant hormones -- Analysis, Plant hormones -- Research, Arabidopsis -- Research, Nucleotide sequencing -- Analysis, Nucleotide sequencing -- Methods, Enzymes -- Synthesis, Enzymes -- Research, Enzymes -- Methods, Biological sciences, Science and technology
Published
2001

52. Light and brassinosteroid signals are integrated via a dark-induced small G protein in etiolated seedling growth

Author

Jeong-Gu Kang, Ju Yun, Dae-Hwan Kim, Kyung-Sook Chung, Fujioka, Shozo, Jeong-ll Kim, Hye-Won Dae, Yoshida, Shigeo, Takatsuto, Suguru, Pill-Soon Song, and Chung-Mo Park

Subjects
Plant cell development -- Physiological aspects, Photochemical research -- Analysis, G proteins -- Physiological aspects, Growth -- Regulation, Biological sciences
Abstract

Research demonstrates that a G-protein, Pra2, is dark-induced and regulates the dark-induced P450 that is involved in the brassinosteroid biosynthesis. Data suggest that Pra2 integrates light and brasinosteroid signals during hypocotyl elongation in dark grown plants.

Published
2001

57. A critical role of sterols in embryonic patterning and meristem programming revealed by the fackel mutants of Arabidopsis thaliana

Author

Jang, Jyan-Chrun, Fujioka, Shozo, Tasaka, Masao, Seto, Hideharu, Takatsuto, Suguru, Ishii, Akira, Aida, Mitsuhiro, Yoshida, Shigeo, and Sheen, Jen

Subjects
Plant cytomorphogenesis -- Research, Botany -- Embryology, Arabidopsis thaliana -- Physiological aspects, Sterols -- Physiological aspects, Plant hormones -- Physiological aspects, Meristem -- Physiological aspects, Dwarf plants -- Physiological aspects, Plant cell differentiation -- Research, Cellular signal transduction -- Research, Biological sciences
Abstract

A role of sterols in embryonic patterning and meristem programming has been found to be critical as can be seen from the fackel mutants of Arabidopsis thaliana. A novel Arabidopsis dwarf mutant, fackel J79 has been studied. Its adult morphology is like that of brassinosteroid-deficient mutants. Embryos are distorted, roots are distorted, and there are supernumerary cotyledons and multiple shoot meristems.

Published
2000

61. The AtGenExpress hormone and chemical treatment data set: experimental design, data evaluation, model data analysis and data access

Author

Goda, Hideki, Sasaki, Eriko, Akiyama, Kenji, Maruyama-Nakashita, Akiko, Nakabayashi, Kazumi, Li, Weiqiang, Ogawa, Mikihiro, Yamauchi, Yukika, Preston, Jeremy, Aoki, Ko, Kiba, Takatoshi, Takatsuto, Suguru, Fujioka, Shozo, Asami, Tadao, Nakano, Takeshi, Kato, Hisashi, Mizuno, Takeshi, Sakakibara, Hitoshi, Yamaguchi, Shinjiro, Nambara, Eiji, Kamiya, Yuji, Takahashi, Hideki, Hirai, Masami Yokota, Sakurai, Tetsuya, Shinozaki, Kazuo, Saito, Kazuki, Yoshida, Shigeo, and Shimada, Yukihisa

Published
2008

62. The Arabidopsis dwf7/ste1 mutant is defective in the [[DELTA].sup.7] sterol C-5 desaturation step leading to brassinosteroid biosynthesis

Author

Choe, Sunghwa, Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, Tissier, Christophe P., Gregory, Brian D., Ross, Amanda S., Tanaka, Atsushi, Yoshida, Shigeo, Tax, Frans E., and Feldmann, Kenneth A.

Subjects
Arabidopsis -- Research, Arabidopsis -- Physiological aspects, Arabidopsis -- Genetic aspects, Biosynthesis -- Research, Gene mutations -- Research, Biological sciences, Science and technology
Published
1999

95. Loose Plant Architecture1(LPA1) determines lamina joint bending by suppressing auxin signalling that interacts with C-22-hydroxylated and 6-deoxo brassinosteroids in rice

Author

Liu, Jing Miao, primary, Park, Soon Ju, additional, Huang, Jin, additional, Lee, Eun Jin, additional, Xuan, Yuan Hu, additional, Je, Byoung Il, additional, Kumar, Vikranth, additional, Priatama, Ryza A., additional, Raj K, Vimal, additional, Kim, Sung Hoon, additional, Min, Myung Ki, additional, Cho, Jun Hyeon, additional, Kim, Tae Ho, additional, Chandran, Anil Kumar Nalini, additional, Jung, Ki Hong, additional, Takatsuto, Suguru, additional, Fujioka, Shozo, additional, and Han, Chang-deok, additional

Published
2016
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96. The Regulation of DWARF4 Expression Is Likely a Critical Mechanism in Maintaining the Homeostasis of Bioactive Brassinosteroids in Arabidopsis1

Author

Kim, Ho Bang, Kwon, Mi, Ryu, Hojin, Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, An, Chung Sun, Lee, Ilha, Hwang, Ildoo, and Choe, Sunghwa

Subjects
Light, Arabidopsis Proteins, Reverse Transcriptase Polymerase Chain Reaction, Protoplasts, Recombinant Fusion Proteins, fungi, Molecular Sequence Data, Arabidopsis, food and beverages, Flowers, Endoplasmic Reticulum, Plant Roots, Cytochrome P-450 Enzyme System, Plant Growth Regulators, Gene Expression Regulation, Plant, RNA, Plant, Seedlings, Steroid Hydroxylases, Homeostasis, Steroids, Plant Shoots, Research Article
Abstract

Mutants that are defective in brassinosteroid (BR) biosynthesis or signaling display severely retarded growth patterns due to absence of growth-promoting effects by BRs. Arabidopsis (Arabidopsis thaliana) DWARF4 (DWF4) catalyzes a flux-determining step in the BR biosynthetic pathways. Thus, it is hypothesized that the tissues of DWF4 expression may represent the sites of BR biosynthesis in Arabidopsis. Here we show that DWF4 transcripts accumulate in the actively growing tissues, such as root, shoot apices with floral clusters, joint tissues of root and shoot, and dark-grown seedlings. Conforming to the RNA gel-blot analysis, DWF4:beta-glucuronidase (GUS) histochemical analyses more precisely define the tissues that express the DWF4 gene. Examination of the endogenous levels of BRs in six and seven different tissues of wild type and brassinosteroid insensitive1-5 mutant, respectively, revealed that BRs are significantly enriched in roots, shoot tips, and joint tissues of roots and shoots. In addition, DWF4:GUS expression was negatively regulated by BRs. DWF4:GUS activity was increased by treatment with brassinazole, a BR biosynthetic inhibitor, and decreased by exogenous application of bioactive BRs. When DWF4:GUS was expressed in a different genetic background, its level was down-regulated in brassinazole resistant1-D, confirming that BRASSINAZOLE RESISTANT1 acts as a negative regulator of DWF4. Interestingly, in the brassinosteroid insensitive2/dwf12-1D background, DWF4:GUS expression was intensified and delocalized to elongating zones of root, suggesting that BRASSINOSTEROID INSENSITIVE2 is an important factor that limits DWF4 expression. Thus, it is likely that the DWF4 promoter serves as a focal point in maintaining homeostasis of endogenous bioactive BR pools in specific tissues of Arabidopsis.

Published
2006

97. CYP72B1 Inactivates Brassinosteroid Hormones: An Intersection between Photomorphogenesis and Plant Steroid Signal Transduction1

Author

Turk, Edward M., Fujioka, Shozo, Seto, Hideharu, Shimada, Yukihisa, Takatsuto, Suguru, Yoshida, Shigeo, Denzel, Megan A., Torres, Quetzal I., and Neff, Michael M.

Subjects
Focus Issue on Light Signaling, Sterols, Cytochrome P-450 Enzyme System, Plant Growth Regulators, Arabidopsis Proteins, Arabidopsis, Morphogenesis, Cloning, Molecular, Promoter Regions, Genetic, Cholestanols, Recombinant Proteins, Signal Transduction
Abstract

Active brassinosteroids, such as brassinolide (BL) and castasterone, are growth promoting plant hormones. An Arabidopsis cytochrome p450 monooxygenase encoded by CYP72B1 has been implicated in brassinosteroid catabolism as well as photomorphogenesis. We expressed CYP72B1 in yeast, coupled with brassinosteroid feeding, and established the biochemical function to be the hydroxylation of BL and castasterone, to give 26-hydroxybrassinolide and 26-hydroxycastasterone, respectively. Brassinosteroid feeding experiments with wild-type Arabidopsis, a CYP72B1 null mutant, and a CYP72B1 overexpression line demonstrated that carbon 26 hydroxylation of active brassinosteroids is an endogenous function of CYP72B1. Seedling growth assays demonstrated that 26-hydroxybrassinolide is an inactive brassinosteroid. Genetic and physiological analysis of the hypocotyl response to exogenous BL and varying intensities of white and monochromatic light suggested that CYP72B1 modulates photomorphogenesis primarily through far-red light and to a lesser extent through blue- and red-light pathways. CYP72B1 transcript accumulation in dark-grown seedlings was organ specific and down-regulated after 1 h of illumination in dim white, red, and blue light, but not far-red light. CYP72B1 translational fusions with the beta-glucuronidase reporter gene demonstrated that protein levels increased in the hypocotyl elongation zone when shifted from the dark to far-red light, but not blue or red light. We propose a model in which Arabidopsis seedling development switches from dark-grown development (skotomorphogenesis) to light-grown development (photomorphogenesis) in part by rapid modulation of brassinosteroid sensitivity and levels. CYP72B1 provides an intersection between the light and brassinosteroid pathways mainly by far-red-light-dependent modulation of brassinosteroid levels.

Published
2003

98. A Putative Role for the Tomato Genes DUMPY and CURL-3 in Brassinosteroid Biosynthesis and Response1

Author

Koka, Chala V., Cerny, R. Eric, Gardner, Randy G., Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, and Clouse, Steven D.

Subjects
Phenotype, Solanum lycopersicum, Molecular Sequence Data, Mutation, food and beverages, Phytosterols, Amino Acid Sequence, Blotting, Northern, Genes, Plant, Sequence Alignment, Research Article
Abstract

The dumpy (dpy) mutant of tomato (Lycopersicon esculentum Mill.) exhibits short stature, reduced axillary branching, and altered leaf morphology. Application of brassinolide and castasterone rescued the dpy phenotype, as did C-23-hydroxylated, 6-deoxo intermediates of brassinolide biosynthesis. The brassinolide precursors campesterol, campestanol, and 6-deoxocathasterone failed to rescue, suggesting that dpy may be affected in the conversion of 6-deoxocathasterone to 6-deoxoteasterone, similar to the Arabidopsis constitutive photomorphogenesis and dwarfism (cpd) mutant. Measurements of endogenous brassinosteroid levels by gas chromatography-mass spectrometry were consistent with this hypothesis. To examine brassinosteroid-regulated gene expression in dpy, we performed cDNA subtractive hybridization and isolated a novel xyloglucan endotransglycosylase that is regulated by brassinosteroid treatment. The curl-3 (cu-3) mutant (Lycopersicon pimpinellifolium ¿Jusl. Mill.) shows extreme dwarfism, altered leaf morphology, de-etiolation, and reduced fertility, all strikingly similar to the Arabidopsis mutant brassinosteroid insensitive 1 (bri1). Primary root elongation of wild-type L. pimpinellifolium seedlings was strongly inhibited by brassinosteroid application, while cu-3 mutant roots were able to elongate at the same brassinosteroid concentration. Moreover, cu-3 mutants retained sensitivity to indole-3-acetic acid, cytokinins, gibberellin, and abscisic acid while showing hypersensitivity to 2, 4-dichlorophenoxyacetic acid in the root elongation assay. The cu-3 root response to hormones, coupled with its bri1-like phenotype, suggests that cu-3 may also be brassinosteroid insensitive.

Published
2000

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