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Abstract Background Effective risk communication is one of the critical strategies in the response to COVID-19. This study examined risk perceptions and attitudinal responses to COVID-19 among the educated section of the society in Ethiopia. Methods An internet-based survey was conducted from April 22 to May 04, 2020, in Ethiopia. A questionnaire addressing the perception of health threat-combination of perceived vulnerability (PV) and perceived seriousness (PS), and perceived efficacy-combinations of perceived response efficacy (PRE), perceived self-efficacy (PSE), and perceived collective efficacy (PCE). The data were analyzed using SPSS 21.0. Descriptive statistics were computed after the standardization of the scores. The scores for overall efficacy and threat were split by median value and response classifications were generated through threat by efficacy interactions. For statistical significance, 95% CI and p-value

This study was initiated with the objective of analyzing land suitability for surface irrigation in Humbo woreda, Southern Ethiopia. For the suitability analysis of surface irrigation, soil type, slope, land cover, and distance from water supply were considered. The suitability factors data such as soil data was obtained from Ethio-soil, slope was derived from DEM-20 meter resolution, land use/cover was classified from satellite image of 2019 (Landsat 7 ETM+), and distance from the River was obtained from Ethio-River map. For each of the criteria suitability map was developed. Slope was classified as 0-2%, 2-5%, 5-8% and 8-15% as highly suitable, moderately, suitable, marginally suitable and not suitable respectively. LULC was classified by unsupervised classification. For the weighted over lay analysis as the most limiting factor slope was given (40%), land use and River proximity given equal weights (30%). The weighted overlay analysis gave irrigable area of 1.22% highly, 50.87% moderately, 45.77% marginally suitable for surface irrigation but 2.13% is not suitable. Thus, in Humbo woreda small area of the land is highly suitable however; more than half of the area is moderately suitable for surface irrigation. Thus, farmers and the government should invest in those suitable areas for surface irrigation.

Abstract The Old World bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae), is a globally distributed agricultural and horticultural insect pest. Despite the economic importance of this insect in Ethiopia, its genetic diversity and demographic history are poorly understood. We examined the nucleotide variation of the mitochondrial cytochrome c oxidase subunit I (COI) gene fragment of 74 H. armigera individuals from six collection sites in Ethiopia. We recorded 15 COI haplotypes in H. armigera, ten globally shared and five exclusive to Ethiopia (HaET15, HaET14, HaET10, HaET7, and HaET4). Haplotype HaET1 was the most widely geographically distributed and frequent (71.62%). Analysis of molecular variance (AMOVA) revealed a high and significant level of variation within H. armigera populations (θST = −0.0135). Negative values of the neutrality test and nonsignificant index of mismatch distribution supported the demographic expansion of H. armigera populations in Ethiopia; furthermore, this was also supported by the nonsignificant values of the sum of squared deviations (SSD) and raggedness index (r). The high genetic variation and population expansion of H. armigera have immense implications for devising locally adapted management strategies in area‐wide integrated pest management IPM programs. However, a comprehensive study of H. armigera genetic diversity and population structure using various molecular markers is needed for future confirmation.

Microwave processing is one of the novel food processing technologies that use electromagnetic radiation in the wavelength and frequency between 1 mm to 1 m and 300 MHz to 300 GHz, respectively. In this review, principles and various applications of microwave technology for food processing are addressed. A systematic literature search was conducted by using, Google Scholar and, Web of Science, Open access theses and dissertations on the principles and application of microwave processing of food were summarized. Additionally, references of each selected publication were examined to get more relevant articles. In microwave processing, the food material absorbs microwave energy directly, and internally, and converts it to heat. The technology is applicable for different unit operations in food industries such as cooking, heating, drying, pasteurization, sterilization, thawing, tempering, baking, blanching, and the extraction of important food biomaterials. Microwave processing is highly advantageous over conventional food processing techniques, in terms of retaining the nutritional content of the food and reducing processing time. Consequently, the breakthrough of the technology in food processing industry has been predicted before. However, the potential of microwave technology is not widely exhausted in Africa. It is associated with a lack of awareness, a priority setting on the application of emerging technologies for safe and high quality value added products; moreover, its high initial investment and operational power cost may become a bottleneck for food processing companies in Africa. Therefore, food innovation centres in Africa should drive high performance standards from technology adoption to improve the application of microwave technology in food industries.

Abstract Rice is an important cereal crop and is cultivated virtually in all agro-ecological zones in Tanzania. However, production capacity is far below the national requirement. One of the major reasons for the low yields of rice in Tanzania is damage by insect pests, particularly rice stem borers. Four rice stem borer species reported to infest rice crop in Tanzania includes the white stem borer, Maliarpha seperatella Ragonot, African pink borer, Sesamia calamistis Hamson, spotted stem borer, Chilo partellus Swinhoe and stalk-eyed fly, Diopsis thoracica Westwood. Inappropriately, the potential for proper management of these stem borers to enhance yield has not been fully realized due to limited information available for the respective stem borer species. Proper management of rice stem borers, therefore, is a pre-requisite for enhanced and sustainable rice production among smallholder farmers that epitomize the rice production industry in Tanzania. In this review, rice stem borers were reviewed with emphasis on occurrence and distribution, nature of damage, biology and life cycle, host plant and management options.

Thermophilic Campylobacter species are common cause of animal and human bacterial diseases with growing resistance to antimicrobials. The aim of this study was to determine the prevalence and antimicrobial susceptibility pattern of Campylobacter species from bovine, knives and personnel in Jimma Town, Ethiopia. Faecal samples and carcasses swabs were collected from cattle systematically selected from the annual plan of Jimma Municipal Abattoir. Personnel hand and knife swabs were collected after slaughtering each selected cattle. A cross-sectional study with systematic sampling method was conducted from October 2019 to September 2020 for the isolation, identification and antimicrobial susceptibility pattern of thermophilic Campylobacter species. Isolation and identification of Campylobacter species were performed according to the techniques recommended by the International Organization for Standardization, and in vitro antibiotic susceptibility testing was screened using the standard agar disc diffusion method as recommended by Clinical and Laboratory Standards Institutions. A total of 684 samples (171 samples from faeces, carcasses, knives and personnel hands, were collected independently). The overall prevalence of thermophilic Campylobacterspecies was 5.6% (38/684). Majority of the isolates were from faecal samples (12.9%, n = 22) followed by carcass swabs(4.1% n = 7), knife swabs(3.5% n = 6) and personnel hand swabs(1.8% n = 3). Isolated and identified species of C.jejuni, C. coli and C. lari accounted for 63.2%, 23.7% and 13.2%, respectively. The isolated Campylobacter species were found to be resistant to Cephalothin (100%), Ampicillin (60.5%), Cefotaxime (60.5%), Chloramphenicol (47.4%) and Tetracycline (42.1%). On the other hand, the isolates were susceptible to Nalidixic acid (86.8%), Ciprofloxacin (86.8%), Sulphamethazole (84.2%), Ceftriaxone (78.9%), Clindamycin (68.4%) and Cefixime (65.8%). 84.2% of the isolates showed multi-drug resistance for three-to-six drug classes. All the C. lari isolates were multidrug resistant. All the three isolated species of Campylobacter were resistant to Cephalothin, and most were multidrug resistant. Isolation of Campylobacter species from faecal, carcass, knife and hand swabs revealed possible risk of contamination and exposure to Campylobacter infection of those who consume raw meat. Therefore, enactment of hygienic practices during the slaughtering process, proper handling and cooking of meat and awareness creation on jurisdictional antibiotic usage are required to avoid Campylobacter infection.

Entomopathogenic fungi (EPF) of different genera are known to have the potential to engage in fungus-plant interactions as fungal endophytes. This hidden endophytic interaction offers several advantages to host plants, such as insect pest management. Hence, this study aimed to explore the endophytic potential and virulence of EPF collections after artificial inoculation. A total of 27 EPF isolates from the genera Beauveria and Metarhizium were screened for virulence. Two inoculation methods (leaf and seed dressing) were used to study the endophytic colonisation potential of the selected isolates. There was a significant variation among the tested isolates in their ability to kill C. partellus larvae. Lower mean percentage mortality was recorded for isolates B4, DS-51-21, and B1,9 which scored 28.01%, 32.29%, and 34.58%, respectively. All the screened EPF isolates were able to colonise maize tissues after artificial inoculation, except for APPRC-34GM. The percent colonisation of maize tissues varied with strains, and delivery methods ranged from a minimum of 0% to a maximum of 53%, where the maximum was recorded by S#10H. Larval mortality after feeding maize leaves inoculated with EPF ranged from 18% to 60%. The findings of this study indicated that Beauveria spp. and Metarhizium spp. have the potential to colonise maize after artificially inoculating and translocating from the site of infection. Hence, the potential to move from the site of infection and larvicidal activity after colonisation may give the advantage to manage insect pests acting on the different parts of maize.

We have assessed the distribution and mating type of chickpea blight (Didymella rabiei (Kov.) v. Arx) from a total of 350 and 272 fields in major chickpea (Cicer arietinum L.) growing areas of Ethiopia for two consecutive cropping seasons 2017 and 2018, respectively. The prevalence of D. rabiei was 20 % for the year 2017 and 7% for 2018. Severity range of 2−9 and incidence of 10–100 % were recorded for both seasons in only 7–20 % of the assed fields. Field prevalence of 2017 and 2018 seasons were significantly different (df = 31; p < 0.001 and df = 31; p < 0.001, respectively). Similarly, the disease prevalence of both seasons was significantly different (df = 31; p < 0.001). The disease prevalence ratio frequency was highly deviated from (1:1) for both seasons (X2 = 213.62 p=

Abstract Objectives Salmonella is the most important causes of foodborne illness especially from poultry and poultry products. So the aim of this study was to carryout phenotypic characterization, antimicrobials susceptibility pattern and risk factors of Salmonella isolates from farms and markets eggs, cloacae swabs of chickens and stool of egg collectors. A cross-sectional study was conducted from January 2018 to September 2018. Samples were, processed; Salmonella was isolated, phenotypically identified by OmniLog and antimicrobials susceptibility were carried out. Result Over all; 11 (2.65%) of Salmonella enterica were phenotypically characterized out of 415 samples from farms egg content (n = 83), farms eggshell (n = 83), cloacae (n = 83), market eggshell (n = 83) and market egg contents (n = 83) with 2.4%, 0%, 2.4%, 4.8% and 3.6% prevalence, respectively. Out of isolates, 8 (72.72%) displayed multidrug resistance. All isolates showed susceptibility to Gentamicin, Kanamycin and Streptomycin. Lack of separating cracked eggs, washing hand, eggs stay longer unsold, and mixing excreta with feed were associated risk factors for Salmonella presence (P-value

Since 2016, fall armyworm (FAW) has threatened sub-Saharan 'Africa's fragile food systems and economic performance. Yet, there is limited evidence on this transboundary pest's economic and food security impacts in the region. Additionally, the health and environmental consequences of the insecticides being used to control FAW have not been studied. This paper presents evidence on the impacts of FAW on maize production, food security, and human and environmental health. We use a combination of an agroecology-based community survey and nationally representative data from an agricultural household survey to achieve our objectives. The results indicate that the pest causes an average annual loss of 36% in maize production, reducing 0.67 million tonnes of maize (0.225 million tonnes per year) between 2017 and 2019. The total economic loss is US$ 200 million, or 0.08% of the gross domestic product. The lost production could have met the per capita maize consumption of 4 million people. We also find that insecticides to control FAW have more significant toxic effects on the environment than on humans. This paper highlights governments and development partners need to invest in sustainable FAW control strategies to reduce maize production loss, improve food security, and protect human and environmental health.

The invasion and wide spread of Spodoptera frugiperda represent real impediments to food security and the livelihood of the millions of maize and sorghum farming communities in the sub-Saharan and Sahel regions of Africa. Current management efforts for the pest are focused on the use of synthetic pesticides, which are often economically unviable and are extremely hazardous to the environment. The use of biological control offers a more economically and environmentally safer alternative. In this study, the performance of the recently described parasitoid, Cotesia icipe, against the pest was elucidated. We assessed the host stage acceptability by and suitability for C. icipe, as well as its ovigenic status. Furthermore, the habitat suitability for the parasitoid in the present and future climatic conditions was established using Maximum Entropy (MaxEnt) algorithm and the Genetic Algorithm for Rule-set Prediction (GARP). Cotesia icipe differentially accepted the immature stages of the pest. The female acceptance of 1st and 2nd instar larvae for oviposition was significantly higher with more than 60% parasitism. No oviposition on the egg, 5th and 6th larval instars, and pupal stages was observed. Percentage of cocoons formed, and the number of emerged wasps also varied among the larval stages. At initial parasitism, parasitoid progenies, time to cocoon formation and overall developmental time were significantly affected by the larval stage. Egg-load varied significantly with wasp age, with six-day-old wasps having the highest number of mature eggs. Ovigeny index of C. icipe was 0.53. Based on the models, there is collinearity in the ecological niche of the parasitoid and the pest under current and future climate scenarios. Eastern, Central and parts of coastal areas of western Africa are highly suitable for the establishment of the parasitoid. The geographic distribution of the parasitoid would remain similar under future climatic conditions. In light of the findings of this study, we discuss the prospects for augmentative and classical biological control of S. frugiperda with C. icipe in Africa.

A cross-sectional study was conducted on selected dairy farms in and around Jimma town, Oromia, southwestern Ethiopia from November 2019 to May 2020 to determine the seroprevalence of Leptospira interrogans serogroup Sejroe serovar Hardjo (L. hardjo). Furthermore, information was gathered on individual animal and herd level by using pretested semistructured questionnaire to assess associated risk factors. A stratified and simple random sampling procedure was used for the selection of dairy farms and individual animal’s, respectively. Indirect enzyme-linked immunosorbent assay (I-ELISA) was used in this study to detect antibody against L. hardjo. Out of 384 animal’s sera, 94 animals were seropositive against L. hardjo antibodies. From 77 dairy farms selected for the study, 57 of them were distinguished as positive for L. hardjo. The overall seroprevalence of leptospirosis caused by L. hardjo was 24.48% (95% CI: 20.18%–28.78%) and 74.03% (95% CI: 64.23%–83.82%) at individual animal and farm level, respectively. The result of multilogistic regression analysis revealed that management system (p 0.05). Management system of the animal, hygienic status of the farm, herd size, age of animals, previous history of abortion, and access of rodents to the farm were identified as potential risk factors of L. hardjo disease occurrence. Thus, limiting rodents contact with cattle and their feed and water as well as good sanitary practices and husbandry management should be undertaken.

Ascochyta blight, also known as chickpea blight, which is caused by the fungal pathogen, Didymella rabiei, is an important disease affecting chickpea (Cicer arietinum L.) in many countries. We studied the genetic diversity and population structure of 96 D. rabiei isolates collected from three geographic populations in Ethiopia using simple sequence repeat (SSR) markers. We confirmed the genetic identity of 89 of the D. rabiei isolates by sequencing their rRNA internal transcribed spacer region genes. The chickpea blight pathogen isolates were genetically diverse, with a total of 51 alleles identified across 6 polymorphic SSR loci, which varied from 3 to 18 (average 8.5) alleles per SSR marker. The observed heterozygosity and expected heterozygosity ranged from 0.01 to 0.92 and 0.19 to 0.86, respectively. The mean polymorphic information content value of the D. rabiei populations was 0.58, with a mean gene diversity of 0.61 among loci. Gene flow (Nm = number of migrants) for the three populations of D. rabiei isolates ranged from 1.51 to 24.10 (average 6.2) migrants/cluster. However, the genetic variation between the D. rabiei populations was small (8%), with most of the variation occurring within populations (92%). Principal component analysis to visualize genetic variation showed that the D. rabiei isolates obtained from most of the chickpea samples formed roughly three groups on a two-dimensional coordinate plane. Similarly, the clustering of individuals into populations based on multi-locus genotypes (using Clumpak) grouped isolates into three clusters but with individual isolate admixtures. Hence, no clear geographic origin-based structuring of populations could be identified. To our knowledge, this is the first report of D. rabiei diversity in Ethiopia. Virulence studies should be conducted to develop chickpea varieties that are resistant to more aggressive pathogen populations.

Abstract Background Evidence exists pointing out how non-adherence to treatment remains a major hurdle to efficient tuberculosis control in developing countries. Many tuberculosis (Tb) patients do not complete their six-month course of anti-tuberculosis medications and are not aware of the importance of sputum re-examinations, thereby putting themselves at risk of developing multidrug-resistant and extensively drug-resistant forms of tuberculosis and relapse. However, there is a dearth of publications about non-adherence towards anti-Tb medication in these settings. We assessed the prevalence of and associated factors for anti-Tb treatment non-adherence in public health care facilities of South Ethiopia. Methods This was a cross-sectional survey using both quantitative and qualitative methods. The quantitative study was conducted among 261 Tb patients from 17 health centers and one general hospital. The qualitative aspect included an in-depth interview of 14 key informants. For quantitative data, the analysis of descriptive statistics, bivariate and multiple logistic regression was carried out, while thematic framework analysis was applied for the qualitative data. Results The prevalence of non-adherence towards anti-Tb treatment was 24.5%. Multiple logistic regression analysis demonstrated that poor knowledge towards tuberculosis and its treatment (AOR = 4.6, 95%CI: 1.4-15.6), cost of medication other than Tb (AOR = 4.7, 95%CI: 1.7-13.4), having of health information at every visit (AOR = 3, 95% CI: 1.1-8.4) and distance of DOTS center from individual home (AOR = 5.7, 95%CI: 1.9-16.8) showed statistically significant association with non-adherence towards anti- tuberculosis treatment. Qualitative study also revealed that distance, lack of awareness about importance of treatment completion and cost of transportation were the major barriers for adherence. Conclusions A quarter of Tb patients interrupted their treatment due to knowledge, availability and accessibility of DOTS service. We recommend creating awareness about anti-Tb treatment, and decentralization of drug pick-ups to the lowest level of health institutions.

Nowadays, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) is becoming a serious insect pest of chickpea in Ethiopia and its management has faced considerable challenges, to improve yields in many crops, in most parts of the world. This experiment was initiated with the objective to evaluate the effectiveness of Azadirachta indica and Milletia ferruginea extracts for the management of H. armigera in chickpea from July to December during 2017 and 2018 cropping season under field conditions. The results revealed that aqueous extracts of M. ferruginea and A. indica at 5% concentration either individually or in combinations at 2.5% concentration of each were more effective in reducing per plant H. armigera larval populations, pod damage with increased the subsequent yields during both cropping seasons as compared to control plot. Comparing the net cost–benefit return of each treatment, during 2017 maximum CBR was from the M. ferruginea 5% treated plot and A. indica 5% treated plots during the 2018 year. Thus, locally available botanical extracts would greatly benefit the resource-poor farmers in chickpea production. Future research attentions and considerations as a part of IPM tools, in pest management, are crucial.

BACKGROUND:Cholera remains a significant public health problem in more than one-third of the countries of the world. Cholera outbreak has become more common in Addis Ababa particularly in the rainy seasons; however, there is a paucity of data on risk factors associated with cholera outbreaks rendering interventions difficult. We investigated the outbreak to identify its etiology, source, risk factors and in order to control the outbreak. METHODS:We compared cases with health center-based unmatched controls (1:2). Cases were patients aged ≥5 years with acute watery diarrhea, with or without vomiting while controls were persons aged ≥5 years without history of acute watery diarrhea. We interviewed our study participants using structured questionnaire to collect demographic and cholera risk factors data. We described the outbreak over time, and then tested our hypotheses using unconditional logistic regression. RESULTS:The outbreak began on 7 September, 2017 reaching its peak on 23 September, 2017 and ended on 01 October, 2017. We identified a total of 25 cases (Median age: 38 years; IQR: 20 years) and recruited 50 controls (Median age: 35 years; IQR: 29 years). All case-patients had acute watery diarrhea and dehydration requiring intravenous fluids. All cases were admitted to cholera treatment center but there were no deaths. Stool and water samples yielded isolates of Vibrio cholerae O1 of serological subtype Ogawa. Consumption of contaminated holy water (AOR: 20.5, 95%CI: 3.50, 119.61) and raw vegetables (AOR: 15.3, 95%CI: 3, 81.51) were independent risk factors whereas washing hands with soap after visiting latrine (AOR: 0.04, 95%CI: 0.01, 0.25) was independent protective factor. CONCLUSION:Our findings demonstrated cholera foodborne transmission via consumption of raw vegetables, and its waterborne transmission via consumption of contaminated holy water. Washing hands with soap after visiting latrine was protective. We recommended cooking of vegetables and promoting hand washing.

The trapping efficacy and specificity of a commercially available two types of traps; funnel and delta traps, two types of lures; botrack (sex pheromone) and femtrack (floral attractant) were evaluated to determine the population dynamics of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) and its effects on non-target insects under field condition of Dandi district during 2018/2019. The result obtained indicates that the funnel-botrack was captured H. armigera moth more profoundly than the other treatments, followed by a femtrack with the same kinds of trap. Likely, funnel-botrack was less effect on non-target insects than the other treatment combinations. Funnel types were more effective than sticky traps in catching H. armigera. Delta traps captured the highest number of non-target insects and recorded the lowest capture of H. armigera moths. Significantly more non-target moths were captured in delta-femtrack and delta-botrack treatments. Across all traps and lure combinations, 6,332 non-target insects were collected, belonging to five major insect orders. The most commonly collected insect orders in descending order were Diptera -> Lepidoptera -> Coleoptera -> Hymenoptera or at a family level, Noctuidae -> Tachinidae -> Muscidae were common in traps baited with H. armigera lures. We noted that, two population peaks in Ethiopia. Our results suggest that the funnel-botrack combination was efficient and specific to capture H. armigera under the agro-ecology of the Dandi district in Ethiopia.

Maize (Zea mays) is an important staple food crop produced by the majority of smallholder farmers that provides household food security through direct consumption and income generation. However, postharvest grain losses caused by insect pests during storage pose a major constraint to household food security. Hermetic storage technology is an alternative method that minimises postharvest losses by depleting oxygen and increasing carbon dioxide levels within the storage container through metabolic respiration of the grains, insects, and microorganism. Maize grain was stored for 180 days in hermetic bags or open-weave polypropylene bags to compare quality preservation when subject to initial grain moisture contents of 12, 14, 16, and 18 percent and infestation by Sitophilus zeamais. The moisture content of grain in hermetic bags remained unchanged while in polypropylene bags decreased. Dry grains (12% moisture content) stored well in hermetic bags and suffered 1.2% weight loss while for equivalent grains in polypropylene bags the weight loss was 35.8%. Moist grains (18% moisture content) recorded the lowest insect density (7 adults/kg grain) in hermetic bags while polypropylene bags had the highest (1273 adults/kg grain). Hermetic and polypropylene bags recorded the lowest (0–4 adults/kg grain) and highest (16–41 adults/kg grain) Prostephanus truncatus population, respectively. Discoloured grains were 4, 6, and 12 times more in grains at 14, 16, and 18 than 12 percent moisture content in hermetic bags. Grains at 18% moisture content recorded significantly lower oxygen (10.2%) and higher carbon dioxide (18.9%) levels. Holes made by P. truncatus in the hermetic bags were observed. In conclusion, storage of moist grains (14–18% moisture content) in hermetic bags may pose health risk due to grain discolouration caused by fungal growth that produces mycotoxins if the grains enter the food chain. The study was on only one site which was hot and dry and further investigation under cool, hot, and humid conditions is required.

The productivity of maize in Ethiopia has remained lower than the world average because of several biotic and abiotic factors. Stemborers and poor soil fertility are among the main factors that contribute to this poor maize productivity. A novel cropping strategy, such as the use of push-pull technology, is one of the methods known to solve both challenges at once. A push-pull technology targeting the management of maize stemborers was implemented in the Hawassa district of Ethiopia with the ultimate goal of increased food security among smallholder farmers. This study evaluated farmers’ perception of push-pull technology based on their experiences and observations of the demonstration plots that were established on-farm in Dore Bafano, Jara Gelelcha and Lebu Koremo village of the Hawasa district in 2016 and 2017. This study examined farmers’ perception of the importance of push-pull technology in controlling stemborers and improving soil fertility and access to livestock feed. In both cropping seasons, except for Jara Gelelcha, the maize grain yields were significantly higher in the climate-adapted push-pull plots compared to the maize monocrop plots. The majority (89%) of push-pull technology-practising farmers rated the technology better than their maize production methods on attributes such as access to new livestock feed and the control of stemborer damage. As a result, approximately 96% of the interviewed farmers were interested in adopting the technology starting in the upcoming crop season. Awareness through training and effective dissemination strategies should be strengthened among stakeholders and policymakers for the sustainable use and scaling-up of push-pull technology.

The fall armyworm (FAW), Spodoptera frugiperda, threatens maize production in Africa. A survey was conducted to determine the distribution of FAW and its natural enemies and damage severity in Ethiopia, Kenya and Tanzania in 2017 and 2018. A total of 287 smallholder maize farms (holding smaller than 2 hectares of land) were randomly selected and surveyed. FAW is widely distributed in the three countries and the percent of infested maize fields ranged from 33% to 100% in Ethiopia, 93% to 100% in Tanzania and 100% in Kenya in 2017, whereas they ranged from 80% to 100% and 82.2% to 100% in Ethiopia and Kenya, respectively, in 2018. The percent of FAW infestation of plants in the surveyed fields ranged from 5% to 100%. In 2017, the leaf damage score of the average of the fields ranged from 1.8 to 7 (9 = highest level of damage), while 2018, it ranged from 1.9 to 6.8. In 2017, five different species of parasitoids were recovered from FAW eggs and larvae. Cotesia icipe (Hymenoptera: Braconidae) was the main parasitoid recorded in Ethiopia, with a percent parasitism rate of 37.6%. Chelonus curvimaculatus Cameron (Hymenoptera: Braconidae) was the only egg-larval parasitoid recorded in Kenya and had a 4.8% parasitism rate. In 2018, six species of egg and larval parasitoids were recovered with C. icipe being the dominant larval parasitoid, with percentage parasitism ranging from 16% to 42% in the three surveyed countries. In Kenya, Telenomus remus (Hymenoptera: Scelionidae) was the dominant egg parasitoid, causing up to 69.3% egg parasitism as compared to only 4% by C. curvimaculatus. Although FAW has rapidly spread throughout these three countries, we were encouraged to see a reasonable level of biological control in place. Augmentative biological control can be implemented to suppress FAW in East Africa.

The fall armyworm, Spodoptera frugiperda, a moth originating from tropical and subtropical America, has recently become a serious pest of cereals in sub-Saharan Africa. Biological control offers an economically and environmentally safer alternative to synthetic insecticides that are being used for the management of this pest. Consequently, various biological control options are being considered, including the introduction of Telenomus remus, the main egg parasitoid of S. frugiperda in the Americas, where it is already used in augmentative biological control programmes. During surveys in South, West, and East Africa, parasitized egg masses of S. frugiperda were collected, and the emerged parasitoids were identified through morphological observations and molecular analyses as T. remus. The presence of T. remus in Africa in at least five countries provides a great opportunity to develop augmentative biological control methods and register the parasitoid against S. frugiperda. Surveys should be carried out throughout Africa to assess the present distribution of T. remus on the continent, and the parasitoid could be re-distributed in the regions where it is absent, following national and international regulations. Classical biological control should focus on the importation of larval parasitoids from the Americas.

Fall armyworm (FAW) was reported for the first time in Africa in 2016. FAW is widely distributed in Ethiopia, causing significant damage to maize. Nine synthetic insecticides belonging to different chemical groups and 11 pesticidal plants (botanicals) were tested for their efficacy against FAW under laboratory, greenhouse, and field conditions. In the laboratory, Radiant, Tracer, Karate, and Ampligo caused over 90% larval mortality 72 h after application. Malathion had moderate activity, causing 51.7% mortality 72 h after application, while Carbaryl was less effective, causing 28% mortality 72 h after application. In the greenhouse experiment, all synthetic insecticides reduced foliar damage to maize compared to the untreated control. Chemical sprays did not affect plant height, stem thickness, or leaf number. The highest fresh weight (471 g) was obtained from plants treated with Radiant. Among the botanicals tested, Azadirachta indica, Schinnus molle, and Phytolacca dodecandra resulted in the highest percentage larval mortality (>95%) 72 h after application. In the field, non-treated control plants showed extensive leaf injury compared to the synthetic insecticide- and botanical-treated plants. The synthetic insecticides and botanicals that showed high efficacy against FAW larvae can be used as components for integrated pest management (IPM) plans for FAW under smallholder farmer conditions in Ethiopia and elsewhere in Africa.

The amount of trace elements present in edible bovine tissues is of importance for both animal health and human nutrition. This study presents data on trace element concentrations in semitendinosus and cardiac muscles, livers and kidneys of 60 zebu (Bos indicus) bulls, sampled at Jimma, Ethiopia. From 28 of these bulls, blood samples were also obtained. Deficient levels of copper were found in plasma, livers, kidneys and semitendinosus muscles. Suboptimal selenium concentrations were found in plasma and semitendinosus muscles. Semitendinosus muscles contained high iron concentrations. Trace elements were mainly stored in the liver, except for iron and selenium. Cardiac muscles generally contained higher concentrations of trace elements than semitendinous muscles except for zinc. A strong association was found between liver and kidney concentrations of copper, iron, cobalt and molybdenum. Liver storage was well correlated with storage in semitendinosus muscle for selenium and with cardiac muscle for cobalt and selenium. Plasma concentrations of copper, selenium, cobalt were well related with their respective liver concentrations and for cobalt and selenium, also with cardiac muscle concentrations. The data suggest multiple trace element deficiencies in zebu cattle in South-West Ethiopia, with lowered tissue concentrations as a consequence. Based on the comparison of our data with other literature, trace element concentrations in selected edible tissues of Bos indicus seem quite similar to those in Bos taurus. However, tissue threshold values for deficiency in Bos taurus cattle need to be refined and their applicability for Bos indicus cattle needs to be evaluated.

Objective: This study aimed to assess the informed consent practice process and associated factors among healthcare workers for major surgical procedures at Gurage zone hospitals, in 2022., Methods and Materials: Institution-based cross-sectional study was employed., Settings: This study was conducted in Gurage zone hospitals., Participants: All healthcare workers who have been working in Gurage zone hospitals of surgical, operation rooms and obstetrics/gynaecology wards had exposure to patients who had undergone surgery during the study period OUTCOME: The primary outcome of the study was the practice of informed consent practice among healthcare workers in Gurage zone hospitals., Result: A total of 448 study participants were involved in this study giving a response rate of 98%. The mean (±SD) age of the study participants was 29.16 (±4.06) years. The mean score of study participants towards the surgical informed consent was 19.5, and 260 (58%, 95% CI: 53.7, 62.5) of them had good practice in the surgical informed consent. In multivariable logistic analysis model, factors like favourable attitude towards informed consent 2.4 (1.556, 3.596), work experience participants 4.9 (2.8, 8.7), adequate knowledge of informed consent 2.5 (1.6, 3.9) and communication challenge 1.6 (1.07, 2.50) were independently associated with the practice of informed consent at a p value of <0.05., Conclusion: More than half of healthcare providers had good practice towards the surgical informed consent process. The practice of informed consent is low, and it is better to work on healthcare providers' in-service training, knowledge and attitude towards informed consent., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2025. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ Group.)

Background: High-risk fertility behavior (HRFB) is a serious public health issue that may influence the country's economic development as well as the health status of mothers mainly in developing countries, like Ethiopia.However, there is a scarcity of evidence about HRFB and associated factors in the study area. Therefore, this study assessed HRFB and associated factors among mothers attending antenatal care in public health facilities in Hossana town, Hadiya zone, Central Ethiopia Region., Methods: A facility-based cross-sectional study was conducted among 276 women who were attending antenatal care follow-up by using a systematic sampling technique from July 01 to 30, 2023. Data were collected through face-to-face interviews. Data entry was done by Epi data version 4.6 and analysis was done by SPSS 25 statistical software. Variables with p-value < 0.25 in bi-variable analysis were moved to multivariable analysis for controlling the possible confounders. In the multivariable analysis, variables with p-values less than 0.05 and a 95% confidence interval were considered statistically significant to HRFB., Results: In the study, it was found that 67.39% of the total participants, specifically women, exhibited high-risk fertility behavior (HRFB) (95% CI: 63.91, 71.01). Among these, 41.90% were identified as having a single high-risk factor (95% CI: 39.78, 44.2), while 25.47% were classified as facing multiple high-risk factors (95% CI: 24.13, 26.8). The research indicated that several factors significantly contributed to maternal HRFB, including lack of antenatal care (ANC) follow-up (AOR: 3.36, 95% CI: 1.78, 6.35), having fewer than four ANC visits (AOR: 2.44, 95% CI: 1.38, 4.32), and a poor knowledge of contraceptives (AOR: 2.89, 95% CI: 1.02, 5.11)., Conclusion: The findings of this study showed that HRFB is high. Almost two out of three women experience HRFB. Having ANC follows- up, number of ANC visits, and knowledge on contraception were the major factors affecting HRFB. HRFB could seriously indicate the development of the country as well as maternal health issues. Therefore, Health education on advantages of ANC follow up and contraceptive use should get due attention., Competing Interests: Declarations. Ethics approval and consent to participate: The study was approved by Ethical review Committee of the Wachemo University received RRC letter from Wachemo university with the registration number 818/14. Informed written and verbal consent was obtained from each participant before interview in respective of considering illiterates. All participants below 18 years old were consent taken from their families. All methods were carried out in accordance with relevant guidelines and regulations. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)

Background: In developing countries evidences regarding pulmonary hypertension (PH) in rheumatic heart disease (RHD) patients are lacking, despite being responsible for significant morbidity and mortality. As a result, identifying the factors that influence PH is crucial to improve the quality of care., Objective: To determine prevalence of pulmonary hypertension and its associated factors among rheumatic heart disease patients at the public hospitals of Bahir Dar city, Ethiopia., Methods: An institution based prospective cross-sectional study was conducted among RHD patients who had follow up at the two public hospitals of Bahir Dar city from January 2022 to December 2023. It involved 310 patients selected by systematic random sampling technique. Pretested, structured, and interviewer-administered questionnaires were used to collect sociodemographic and diseases related parameters.Transthoracic echocardiography by cardiologist was used to assess PH. Data were entered using Epidata Manager version 4.6 and analyzed using SPSS version 27. Multivariate logistic regression analysis was used to identify determinants of PH, considering with a p-value of < 0.05 as statically significant, with a 95% confidence interval., Results: The mean systolic pulmonary arterial pressure (sPAP) of the participants was 50.2 mmHg [SD ± 25.0 mmHg]. The prevalence of PH among RHD patients was 56.5% (95% CI 50.9 - 61.9) from which 51.4% had severe PH. Severe mitral valve stenosis (AOR 7.8, 95% CI 2.4-25.7), duration of illness ≥ 3 years (AOR 7.7, 95% CI 2.1-28.5), and diuretics use (AOR 5.6, 95% CI 2.2-14.3) were factors associated with PH. In contrast, valvular intervention (AOR 0.06, 95% CI 0.01-0.29) and LVEF ≥ 50% (AOR 0.14, 95% CI 0.02-0.81) were found to be protective factors., Conclusions: The prevalence of PH among RHD patients in Ethiopia is high and it's associated with delayed presentation & complications. Special attention should be paid to early surgical or percutaneous valvular intervention for those who have indication, before they develop permanent cardiac remodeling and LVFE become reduced. As a result, access to valvular intervention need to be addressed to improve PH related morbidity & mortality among RHD patients in Ethiopia., Competing Interests: Declarations. Ethics approval and consent to participate: This study was conducted according to declarations of Helsinki. Ethical clearance was approved by the Research Ethical Review Board of College of Medicine and Health Sciences, Bahir Dar university (protocol number 802/2023). Written informed consent was obtained from the participants including legal guardians and or parents, and patient data confidentiality was respected at all levels from patient interview, chart retrieving and data analysis which was handled by the investigators. During the data collection process, those patients who were found to have pulmonary hypertension were linked to the treating physicians for any farther management. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)

This study investigates the optimization of four gluten free flours namely sorghum, rice, teff flours, and 3% flaxseed flour blends to enhance the quality injera , which was traditionally baked with only pure teff. Utilizing a D-optimal mixture design, ratios were varied (sorghum 43%-50%, rice 20%-27%, teff 23%-30%). Methods followed AOAC and AACC standards, analyzed using Minitab 19.2 software with one-way and two-way ANOVA. Results show flaxseed supplementation significantly improves sorghum-based injera's texture and sensory attributes, approaching teff injera quality. Hedonic ratings (color, rollability, softness, taste, eye distribution, mouthfeel, and overall acceptability) were favorable. Physical texture remained stable during storage, with variable titratable acidity among blends. This research supports integrating flaxseed flour in grain blends to enhance injera's nutritional and sensory qualities, proposing applications in both household and industrial settings. The purpose of this study is to investigate the supplementation of flaxseed flour in a blend of sorghum, rice, and teff flours, for its performance to improve the overall acceptability, and sensory appeals of the produced injera., Competing Interests: The authors declare no conflicts of interest., (© 2025 The Author(s). Food Science & Nutrition published by Wiley Periodicals LLC.)

Background: Despite the availability of vaccination and early treatment, cervical cancer remains a significant public health concern globally, particularly in Sub-Saharan Africa, where access to screening and treatment is often limited., Methods: In this study, researchers conducted a survey of four international databases-Medline/PubMed, Scopus, Web of Science, and Henare-along with Google Scholar to search for gray literature. The keywords used for searching the international databases included "Uterine Cervical Neoplasms [Mesh]," "Survival OR Survival Analysis OR Survival Rate," and "Sub-Saharan countries" (including the names of specific countries). Six researchers independently screened and extracted data from the articles. All studies published in English were included without restriction and assessed for quality using the adapted Newcastle-Ottawa Scale for cohort and cross-sectional studies. The results of this systematic review were reported in accordance with the PRISMA checklist., Results: Out of the 2,180 articles initially identified, 23 were deemed eligible and reported on the survival status of patients with cervical cancer in Sub-Saharan Africa. This study assessed the multi-year survival rates (1, 2, 3, 4, and 5 years) of patients with cervical cancer. Based on the random-effects model, the overall pooled 1-year survival was 65.0% [95% confidence interval (CI), 52-78] with I² = 99.31 and p-value < 0.001. The 2-year survival rate was 60% (95% CI, 46-74) with I² = 99.12 and p-value < 0.001, the 3-year survival was 48% (95% CI, 35-62) with I² = 98.45 and p-value < 0.001, the 4-year survival was 42.9% (95% CI, 32.7-53.1) with I² = 96.80 and p-value < 0.001, and the 5-year survival was 35% (95% CI, 27-44) with I² = 98.74 and p-value < 0.001., Conclusions: This systematic review and meta-analysis found that the survival rates for patients with cervical cancer in Sub-Saharan Africa are much lower than the global averages. The results show that the 5-year survival rate can be as low as 35%, highlighting serious challenges in managing cervical cancer in this region. To address this issue, collaboration among governments, healthcare providers, and international organizations is essential to enhance the availability and quality of care. Future research should focus on developing effective early detection and treatment strategies and monitoring long-term survival outcomes., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2025 Emagneneh, Mulugeta, Ejigu, Alamrew, Hiwot and Feleke.)

Background: Teachers play important roles in influencing vaccine uptake decisions and should serve as examples for their students by willingly getting vaccinated. Given the low vaccination rates in this area, it is crucial to explore teachers' willingness to get vaccinated and emphasize their role in promoting vaccine acceptance., Objective: The main objective of this study was to assess the willingness of teachers to receive the COVID-19 vaccine and its associated factors in Dambi Dollo town., Methods: A school-based cross-sectional study was conducted from February 1 to March 30, 2022. Data were collected using a self-administered questionnaire and analysed with SPSS version 23.0. The dependent variable was willingness to receive the COVID-19 vaccine. Variables that showed an association with the dependent variable in the bivariable analyses at a p-value of 0.25 were entered into a multiple stepwise logistic regression model. A p- value of 0.05 was considered statistically significant in this study's multivariable analysis to determine the presence of significant association between the dependent and the independent variables. Adjusted Odds ratios, along with the corresponding 95% confidence intervals were used to interpret the findings., Results: A total of 247 teachers participated in this study. About 92% of the teachers in Dambi Dollo town have heard COVID-19 vaccine and 67.2% of them knew that the vaccine can prevent the COVID-19 disease. On the other hand, 51% (95%CI: 44.8, 57.2) of the respondents had good knowledge about the COVID-19 vaccine. From the total 247 participants, 68.4% (95%CI: 62.5, 74.3) were willing to receive the vaccine immediately while the remaining were either not willing or not ready at the time of data collection. Those participants having good knowledge of the vaccine were about six times more willing to get vaccinated (AOR = 5.85, 95%CI: 2.74, 12.47) in comparison with those having poor knowledge., Conclusion: In conclusion, the level of willingness to receive the COVID-19 vaccine among school teachers in Dambi Dollo town was 68.4. Followers of Protestant religion and Muslims were less willing compared with Orthodox Christians while the participants with good knowledge were more willing to be vaccinated., Competing Interests: Declarations. Ethics approval and consent to participate: This study was conducted in accordance with the ethical principles of the Declaration of Helsinki. Written informed consent was obtained from each participant to ensure they were aware of their right to participate or not, and the study continued with those who agreed to participate. On the other hand research ethical approval letter was obtained from the Dambi Dollo University Institutional Research Ethics Review Board. Confidentiality and privacy were maintained by excluding the names and IDs of study participants from the questionnaire. Those who did not volunteer to participate in the study were respected not to participate by their decision. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

Background: Malaria is a serious global public health problem, which is caused by genus Plasmodium. Resistance of the human malaria parasite to antimalarial drugs is a public health concern in malaria endemic countries. Chloroquine is resistant for both P. vivax and P. falciparum. Chloroquine resistance is understood throughout all of Africa's P. falciparum endemic regions. Molecular markers play a crucial role in tracking and understanding the prevalence of antimalarial drug resistance. Currently, there is inadequate information on the prevalence of molecular markers of chloroquine resistance in malaria parasites., Objective: This systematic review and meta-analysis aimed to determine the pooled prevalence of molecular markers of chloroquine resistance in malaria parasites in East Africa., Methods: Systematic search was performed to retrieve articles from PubMed, Scopus, Science Direct, and the Google Scholar search engine. Twenty potential studies that provided important data on markers of chloroquine resistance in malaria parasites were systematically reviewed and analyzed. Five antimalarial drug resistance markers of chloroquine resistance were extracted separately into Microsoft Excel and analyzed using STATA 17.0. The Inverse of variance (I 2 ) was done to evaluate heterogeneity across studies. The funnel plot and the Egger's test were used to determine the existence or absence of publication bias. A trim-and-fill meta-analysis was carried out to generate a bias-adjusted effect estimate. A random effect model was used to determine the pooled prevalence of molecular markers associated with chloroquine resistance in malaria parasites. Subgroup analysis was performed based on country and year of publication., Results: A total of 20 studies were included for this systematic review and meta-analysis. The molecular markers like K76T, 76T, N86Y, Y184F, and 86Y were selected for meta-analysis. From this meta-analysis, the pooled prevalence of K76T, 76T, N86Y, Y184F, and 86Y was 34.5%, 47.3%, 43.8%, 58.3%, and 29.2%, respectively. After adjusting for publication bias, the estimated pooled prevalence of K76T, 76T, N86Y, Y184F, and 86Y were 34.5%, 47.3%, 43.8%, 58.3%, and 29.2%, respectively. Meta-analysis showed a significant difference in all molecular marker prevalence like K76T and 86Y among studies on year of publication except 76T, N86Y, and Y184F. In addition, the meta-analysis showed a significant difference in all molecular marker prevalence like K76T, 76T, N86Y, Y184F, and 86Y among studies at the country level., Conclusions: The findings of this systematic review and meta-analysis concerning the molecular markers of chloroquine resistance of malaria parasites in East Africa revealed a significant prevalence of antimalarial drug resistance markers of chloroquine. As a result, continued surveillance and monitoring of the prevalence of molecular markers of chloroquine resistance, identification and limitation of drug-resistant malaria parasite strains, and development of new antimalarial treatments are required to guide malaria treatment policies, interventions, control, and elimination of malaria worldwide., Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interests., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Introduction: Promoting the involvement of male partners in skilled delivery care is a strategy supported by the World Health Organization (WHO) to improve maternal and infant health outcomes. This systematic review and meta-analysis aimed to estimate the pooled prevalence of male partner involvement in delivery care service in Ethiopia and its contributing factors., Methods: We retrieved observational studies conducted in Ethiopia from PubMed, Google Scholar, Embase, Cochrane Library, Hinari, and Mednar using Boolean search terms. The Newcastle Ottawa 2016 Critical Appraisal Checklist assessed the methodological quality of the studies. Publication bias was evaluated with a funnel plot and Egger's test, and heterogeneity was checked using the I-squared test. Data were extracted into Microsoft Excel and analyzed using Stata 11 software., Results: 10 articles with 5,307 participants were included for analysis. The pooled prevalence of male partner involvement in delivery care service was 52.99% (95% CI: 40.63-65.35). Paternal secondary education and above (OR 1.99, 95% CI: 1.69, 2.30), paternal knowledge (OR 3.129, 95% CI: 1.901, 4.356), partner attitude (OR 2.39, 95% CI: 1.45-3.34), having ANC accompanying experience (OR 8.09, 95% CI: 3.14, 19.32), and urban residence (OR 2.12, 95% CI: 1.61, 2.64) were significantly associated with male partner involvement in delivery care service in Ethiopia., Conclusion: This study found that more than half of male partners in Ethiopia were involved in delivery care services. The key contributing factors for male partner involvement in delivery care services were paternal secondary education and above, paternal knowledge, partner attitude, ANC accompanying experience, and urban residence. Campaigns should be organized to improve knowledge, attitude, and effectively recognize men's involvement in skilled birth care., Competing Interests: Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

Background: Safe motherhood programs must include both readiness for complications and childbirth. Birth preparedness and complication readiness (BPCR) is a comprehensive approach that helps resolve delays in deciding to seek care for obstetric problems., Objectives: To identify the determinants of BPCR practice among reproductive age group women in Africa., Design: Systematic Review and Meta-Analysis., Data Sources and Methods: Preferred Reporting Items for Systematic Reviews and meta-analysis (PRISMA) were followed and databases such as MEDLINE, PubMed, Scopus, Hinari, Google Scholar, and Web of Science were used to find the available studies. Adjusted Odds Ratio (AOR) with 95% Confidence Interval (CI) was used to identify associated factors. I2 statistics, funnel plot, and Egger test were used to assess the studies' heterogeneity and publication bias., Results: In this review, a total of 32 studies with 17,787 sample sizes were included. Knowledge of BPCR (AOR = 2.12, 95% CI: 1.44, 3.13), knowledge of danger signs during pregnancy (AOR = 1.60; 95% CI: 1.37, 1.88), Knowledge of labor and delivery danger signs (AOR = 1.44; 95% CI: 1.28, 1.62), Knowledge of postpartum danger signs (AOR = 1.40; 95% CI: 1.21, 1.63), urban residency (AOR = 1.32; 95% CI: 1.03, 1.70), Antenatal Care follow-up (AOR = AOR = 1.52;95% CI:1.42, 1.63), history of stillbirth (AOR = 1.59; 95% CI: 1.36, 1.86), parity (AOR = 1.76; 95% CI: 1.16, 2.66) and secondary or higher educational status (AOR = 1.51: 95% CI: 1.35, 1.68) were the determinants of BPCR practice., Conclusion: BPCR practice among African women of reproductive age has been greatly affected by antenatal care visits, urban residency, knowledge of danger during pregnancy, labor, and postpartum, history of stillbirth, primiparity, and secondary or higher education. Focused interventions that address the identified factors may enhance BPCR practices and maternal health outcomes in Africa., Competing Interests: Declarations Ethics approval and consent to participate Not applicable. Consent for publication Not applicable. Conflict of interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024. The Author(s).)

Introduction: Breast cancer remains a significant public health issue, with delayed medical attention often leading to advanced stages and poorer survival rates. In East Africa, evidence on the prevalence and factors contributing to the delayed presentation of breast cancer is limited. As a result, this study aims to assess the pooled prevalence of delayed breast cancer presentation and identify contributing factors in East Africa., Methods: We conducted a systematic review of observational studies from East Africa using PubMed, Google Scholar, Embase, Cochrane Library, Hinari, and Mednar databases. The Newcastle Ottawa 2016 Critical Appraisal Checklist assessed methodological quality. Publication bias was evaluated using a funnel plot and Egger's test, and heterogeneity was examined with the I-squared test. Data were extracted with Microsoft Excel and analyzed using Stata 11., Results: The pooled prevalence of delayed presentation among breast cancer patients in East Africa was 61.85% (95% Confidence Interval: 48.83%-74.88%). Significant factors contributing to delayed presentation included visiting traditional healers (Adjusted Odds Ratio: 3.52; 95% CI: 1.43-5.59), low educational levels (Adjusted Odds Ratio: 3.61; 95% CI: 2.39-4.82), age>40 years (Adjusted Odds Ratio 1.87; 1.03, 2.71), absence of breast pain (Adjusted Odds Ratio 2.42; 1.09, 3.74), distance >5km away from home to health institution (Adjusted Odds Ratio 2.89; 1.54, 4.24), and rural residence (Adjusted Odds Ratio: 3.33; 95% CI: 2.16-4.49)., Conclusion: This meta-analysis's findings demonstrated that over half of breast cancer patients in East Africa delayed detection. Significant factors associated with delayed presentation include age over 40 years, illiteracy, rural residence, use of traditional healers, distance greater than 5 km from a health facility, and absence of breast pain. Healthcare stakeholders and policymakers must be focused on raising awareness and educating people to encourage early detection and prompt therapy., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Mulugeta et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Urban expansion in developing countries like Ethiopia is causing political turmoil in peri-urban areas due to inadequate compensation for evicted farmers because of the absence of a land market, which is prohibited by Ethiopia's land policy. This paper applies the contingent valuation method to measure the compensation value considered acceptable to those who are evicted from their farm land. The west Gojjam and Awi zones of Amhara region, Ethiopia are chosen for the case study based on 393 completed questionnaires. It has been demonstrated that a willingness to accept compensation is essential for maintaining sustainable livelihoods. The study applied single-bounded contingent valuation and double-bounded contingent valuation methods to estimate the mean willingness to accept compensation value. Based on the finding, double-bounded contingent valuation could be more optimal for the welfare of the evictees in detecting and correcting respondent's bias due to its narrow confidence interval. Pilot survey was employed to ensure reliability and validity of the methods employed. Therefore, the optimal bid vector for multiplying the average land output preceding three years of eviction is 29.27. Additionally, variables such as initial bid, age, education, and total annual income significantly affect the willingness to accept compensation. The study recommends that federal and local governments should provide adequate compensation offers that prioritize the well-being of peri-urban communities during urban expansion programs, as farmers are losing their most valuable asset. This is crucial for sustainable living and poverty reduction among those who have been evicted., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Reliable detection of Aquaporin-4 (AQP4) antibodies is crucial for diagnosing Neuromyelitis Optica spectrum disorder (NMOSD). However, cell-based assays, the most reliable approach, are limited by inadequate instruments. This study reports the use of silver metal-organic gels (Ag-MOGs) as coreactants in a single-electrode electrochemical system (SEES)-based electrochemiluminescence (ECL) immunosensor for multiplex detection of AQP4 antibodies. The immunosensor was constructed by modifying the carbon nanotube single-electrode with Ag-MOGs, incubating it with AQP4 peptides, and ultimately enabling the immobilization of AQP4 antibodies. Voltage-induced potential gradients at the electrode triggered the Ru(bpy) 3 2+ ECL reaction, and reduced emissions caused by AQP4 antibodies were recorded using a smartphone. Under optimal conditions, the immunosensor exhibited a strong linearity (10-1000 ng/mL) with a detection limit of 2.8 ng/mL. Validation of its accuracy, precision, dilutability, and selectivity confirmed robust performance across the diverse parameters. Furthermore, it successfully detected AQP4 antibodies in serum samples from seropositive NMOSD patients. The platform's single electrode design and multiplex capability make it simple, fast and cost-effective. Enhanced accessibility and user-friendliness could position this system as a transformative tool for improving disease diagnosis and treatment, particularly in resource-limited regions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025 Elsevier B.V. All rights reserved.)

Ethnopharmacological Relevance: The emergence of antibiotic-resistant bacteria has intensified the search for novel antidiarrheal drug. Plant-derived extracts offer promising alternatives due to their ability to modulate gut motility and enhance water absorption. The leaves of Leucas deflexa Hook.f., a plant native to Ethiopia, have been traditionally utilized in the treatment of diarrheal diseases. Nonetheless, its traditional application has yet to be scientifically confirmed., Aim of the Study: Hence, the aim of the present study was to evaluate antidiarrheal activity of the crude extract and fractions of the leaf of Leucas deflexa Hook.f (Lamiaceae)., Materials and Methods: Healthy Swiss albino mice were randomly divided into five groups per experimental model: castor oil-induced diarrhea, gastrointestinal motility, and anti-enteropooling assays. The control group received distilled water (10 ml/kg), while the positive control was treated with loperamide (3 mg/kg). The remaining groups were administered various dosages (LD100, LD200, and LD400 mg/kg) of a hydroalcoholic extract or its fractions from Leucas deflexa Hook.f., Result: The crude extract and chloroform fraction substantially (P < 0.001) delayed the onset of diarrhea, reduced fecal frequency, and decreased total fecal weight at all administered doses compared to the negative control. The butanol and aqueous fractions exhibited a substantial antidiarrheal effect at doses of 200 mg/kg and 400 mg/kg, respectively. All treatments, including the crude extract, chloroform, butanol, and aqueous fractions, substantially (P < 0.001) reduced the volume and weight of intestinal contents at various doses. Additionally, the chloroform, aqueous fraction, and crude extract at doses of 100, 200, and 400 mg/kg substantially inhibited gastrointestinal motility compared to the negative control., Conclusion: The results of this study corroborate the traditional use of Leucas deflexa leaves in the treatment of diarrhea. These findings give a scientific justification for further exploration of the underlying mechanisms and potential therapeutic applications of this plant extract., Competing Interests: Declaration of competing interest We have no conflicts of interest to disclose associated with this publication This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors that could have influenced its outcome. We understand that the Corresponding Author is the sole contact for the Editorial process. He is responsible for communicating with the other authors about progress, submissions of revisions and final approval of proofs., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Background: Co-inhibitory receptors (immune checkpoints) regulate activated immune cells. Their expression on T cells can limit host defense. We hypothesized that chronic Leishmania donovani infection in patients with visceral leishmaniasis (VL) leads to expression of co-inhibitory receptors that could be markers of treatment response and clinical outcome., Method: A prospective cohort of 21 subjects with VL (7 with HIV coinfection) and 10 controls was established to measure T-cell expression of co-inhibitory receptors (PD-1, Tim-3, LAG-3, CTLA-4, and TIGIT) by flow cytometry in discarded remnants of diagnostic splenic or bone marrow aspirates and peripheral blood collected before and after treatment. Plasma levels of soluble co-inhibitory proteins (sPD-1, sTim-3, sLAG-3, and sCTLA-4) and selected cytokines were determined by immunoassay., Results: Expression of co-inhibitory receptors in peripheral blood T cells generally reflected findings in spleen and bone marrow aspirates. PD-1 and Tim-3 were upregulated in CD4+ T cells in HIV-negative and HIV-positive subjects with VL compared to controls. CD8+ T cells from HIV-negative subjects with VL displayed a similar pattern. Plasma levels of sPD-1 and sTim-3 were also greater in VL patients than controls. CD8+ and CD4+ T cells coexpressing PD-1 and Tim-3 showed considerable decline with treatment. Mortality in HIV-negative VL patients was associated with increased CD8+ T cells coexpressing Tim-3 and PD-1, triple-positive CD4+ and CD8+ T cells (PD-1 + Tim-3 + LAG-3 + ), and elevated sLAG3., Conclusions: Tim-3 and PD-1 expression on CD4+ and CD8+ T cells, and increased plasma sLAG-3, were markers of treatment response and clinical outcome in patients with VL., Competing Interests: Potential conflicts of interest. All authors: No reported conflicts. The funders had no role in the study design, data analyses, interpretation of data, writing the manuscript, and decision to publish the results., (© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America.)

Salmonellosis represents a significant economic and public health concern for the poultry industry in Africa, leading to substantial economic losses due to mortality, reduced productivity, and food safety problems. However, comprehensive information on the burden of poultry salmonellosis at the continental level is scarce. To address this gap, a systematic review and meta-analysis were conducted to consolidate information on the prevalence and circulating serotypes of poultry salmonellosis in African countries. This involved the selection and review of 130 articles published between 1984 and 2021. A detailed systematic review protocol was structured according to Cochrane STROBE and PRISMA statement guideline. From the 130 selected articles from 23 different African countries, the overall pooled prevalence estimate (PPE) of poultry salmonellosis in Africa was found to be 14.4% (95% CI = 0.145-0.151). Cameroon reported the highest PPE at 71.9%. The PPE was notably high in meat and meat products at 23%. The number of research papers reporting poultry salmonellosis in Africa has shown a threefold increase from 1984 to 2021. Salmonella Enteritidis and Typhimurium were the two most prevalent serotypes reported in 18 African countries. Besides, Salmonella Kentucky, Virchow, Gallinarum, and Pullorum were also widely reported. Western Africa had the highest diversity of reported Salmonella serotypes (141), in contrast to southern Africa, which reported only 27 different serotypes. In conclusion, poultry salmonellosis is highly prevalent across Africa, with a variety of known serotypes circulating throughout the continent. Consequently, it is crucial to implement strategic plans for the prevention and control of Salmonella in Africa. RESEARCH HIGHLIGHTS The pooled sample prevalence of poultry salmonellosis in Africa is high (14.4%).The highest PPE was recorded in meat and meat products. Salmonella serotypes of zoonotic importance were found in all sample types. Salmonella Enteritidis and Typhimurium are common serotypes spreading in Africa.

Background: Birth before arrival (BBA) constitutes a high-risk newborn population with high perinatal morbidity and mortality. In Ethiopia, most studies and health surveys consider only home and hospital deliveries but do not consider deliveries that take place between the house and health facility. The aim of this study was to assess the prevalence of BBA and its associated factors among postpartum women in Lemo woreda, Hadiya Zone, SNNPR, Ethiopia, 2023., Methods: A community-based cross-sectional study was conducted among postpartum women in Lemo woreda, Hadiya Zone, SNNPR, Ethiopia, from April 5 to May 20, 2023. Three hundred eighty-two postpartum women who gave birth 6 months prior to this study were included. Twelve out of 36 kebeles were selected randomly, and simple random sampling was employed for the selection of participant women. An interviewer-administered questionnaire was used for data collection. A binary logistic regression analysis was computed, and variables with a p value of <0.25 were included in the final multivariable logistic regression analysis. Model fitness was checked via the Hosmer-Lemeshow goodness-of-fit test ( x 2  = 16.04, p value = 0.250). Statistical significance was declared via odds ratios and 95% confidence intervals at a p value <0.05., Results: The prevalence of BBA among women who gave birth in the last 6 months preceding this study in the study area was 15.2% (95% CI: 11.8, 19.1%). In the multivariable analysis, the variables associated with birth before arrival in the final model were having no antenatal care (AOR = 2.63; 95% CI: 1.23, 5.63), having a female autonomy status (AOR = 3.32; 95% CI: 1.12, 9.89), not being knowledgeable about labor symptoms (AOR = 2.15; 95% CI: 1.11, 4.18), and having birth preparedness toward the index birth (AOR = 0.13; 95% CI: 0.05, 0.35)., Conclusion: The prevalence of BBA in the study area was unacceptably high. A statistically significant association was observed between birth before arrival and having no antenatal care, dependent women's autonomy status, being not knowledgeable about labor symptoms, and having birth preparedness toward the index birth., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Amanuel, Desalegn, Lukas and Yohannes.)

Background: Over the past years, there has been a growing concern that a considerable amount of anti-malarial supply in the underdeveloped world particularly in the private sector, is of poor quality. The World Health Organization (WHO) has received about 1500 reports that mentions instances of substandard and falsified products since 2013. The majority of the reports concerned antibiotics and anti-malarials. The majority of reports (42%) originate from the WHO African region., Objective: This study intends to assess the quality of the most widely used anti-malarial medications [artemether-lumefantrine tablets, chloroquine phosphate tablets, primaquine phosphate tablets, artesunate, and artemether injections] in Gambella, South-West, Ethiopia., Methods: A total of 52 samples were collected on June 2022 from Gambella National Regional State, Ethiopia. Half of the districts (six) located in the four zones of the region were chosen using simple random sampling technique. All drug retail outlets available in the selected districts (locally known as woredas) were included. The samples were subjected to visual inspection with a tool adopted from the joint WHO/FIP/ USP checklist. The pharmacopeial tests for identification, uniformity of dosage forms, assay, thickness, diameter, hardness, friability, disintegration test, dissolution, and sterility tests were carried out according to the USP 44-NF 39 and International Pharmacopoeia 11th edition, 2022 monographs., Results and Discussion: Only 25% of the samples were registered on the Ethiopian Food and Drug Authority (EFDA's) electronic regulatory/ registration system (ERIS). Besides, 88.8% of artemether injection products were presented in clear glass ampoules. This might expose the products to photochemical degradation that leads to in loss of anti-plasmodial activity. In addition, 50% of the artemether products assessed were not bioequivalent with the comparator product in the in vitro dissolution comparison tests. Overall, the study findings reveal a high prevalence (58.3%) of substandard anti-malarial drugs in the region. The stated percent of the samples had failed in one or more of the quality test parameters assessed in this study., Conclusion: The study findings reveal a high prevalence (58.3%) of substandard anti-malarial drugs in the region. Only a quarter were registered and 38% of the unregistered products failed the quality tests. Hence, the national, regional medicine regulatory bodies and other stake holders should perform the required roles to circumvent presence of Substandard and Falsified (SF) anti-malarial drugs in the study sites., (© 2024. The Author(s).)

Background: Antibiotic resistance is a global health concern, and its prevalence among older adults and Alzheimer's disease (AD) patients is gaining attention. Understanding the extent of antibiotic resistance in these populations is critical for designing targeted interventions., Objective: The objective of this systematic review and meta-analysis was to determine the prevalence of antibiotic resistance in older adults and AD patients with a focus on quantitative studies in order to provide comprehensive insights into the current landscape., Methods: To identify relevant studies, we conducted a thorough search of the PubMed, Scopus, CINAHL, and Web of Science databases. Only studies involving adults and AD patients, published in English, and reporting quantitative data on antibiotic resistance prevalence were considered. The Risk of Bias In Non-randomized Studies of Interventions (ROBINS-I) tool was used to assess quality. The data was summarized by using Revman 5.4.1., Results: A total of six studies met the final criteria for selection and results from the meta-analysis found a pooled prevalence odds ratio of OR = 1.27 (95% CI: [0.99, 1.63], Z  = 1.87, p  = 0.06). The studies showed significant heterogeneity (I2 = 100%, p  < 0.00001), emphasizing the need for cautious interpretation., Conclusions: The findings indicate a potential trend of increased antibiotic resistance in older adults and AD patients, though statistical significance was not achieved for both. The significant heterogeneity highlights the complexity of resistance patterns in these populations, necessitating additional research for tailored interventions., Competing Interests: The authors have no conflict of interest to report., (© 2024 – The authors. Published by IOS Press.)

Visceral leishmaniasis (VL) is a neglected tropical disease caused by parasites from the Leishmania (L.) donovani complex. VL is characterised by uncontrolled parasite replication in spleen, liver and bone marrow, and by an impaired immune response and high systemic levels of inflammation. Monocytes have been poorly characterised in VL patients. The aim of this study was to evaluate the expression levels of markers involved in the regulation of T cell responses on different subsets of monocytes from the blood of VL patients and healthy non-endemic controls (HNEC). Monocytes can broadly be divided into three subsets: classical, intermediate and non-classical monocytes. Our results show that the percentages of all three subsets stayed similar at the time of VL diagnosis (ToD) and at the end of anti-leishmanial treatment (EoT). We first looked at co-stimulatory receptors: the expression levels of CD40 were significantly increased on classical and intermediate, but not non-classical monocytes, at ToD as compared to EoT and HNEC. CD80 expression levels were also increased on intermediate monocytes at ToD as compared to EoT and HNEC, and on classical monocytes only as compared to HNEC. The levels of CD86 were similar at EoT and ToD and in HNEC on classical and intermediate monocytes, but significantly higher at EoT on non-classical monocytes. We also looked at an inhibitory molecule, PD-L1. Our results show that the expression levels of PD-L1 were significantly higher on all three monocyte subsets at ToD as compared to HNEC, and to EoT on classical and intermediate monocytes. These results show that monocytes from the blood of VL patients upregulate both co-stimulatory and inhibitory receptors and that their expression levels are restored at EoT., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Adem et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)