1,680 results on '"TRAF6"'
Search Results
52. Structure and function of the pseudokinases IRAK2 and IRAK3 and their potential as drug targets
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Lange, Sven Manfred, Cohen, Philip, Kulathu, Yogesh, and Nelen, Marina
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572 ,pseudokinase ,IRAK ,TRAF6 ,Drug discovery ,crystallography ,Structural biology ,Immunity - Abstract
The Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1R) are critical components of the innate immune system that recruit the adapter protein MyD88 (myeloid differentiation primary response gene 88), which serves as an assembly platform for members of the interleukin-1 receptor associated kinase (IRAK) family to form an oligomeric signalling complex called the Myddosome. Subsequently, IRAK kinases and pseudokinases recruit and activate a number of E3 ubiquitin ligases that trigger downstream signalling events to induce the production of pro- and anti-inflammatory mediators. The emergence of crystal structures and development of specific inhibitors of the catalytically active IRAK family members, IRAK1 and IRAK4, have advanced our understanding of their roles in the TLR/IL-1R signalling pathway. However, there is no structural or mechanistic information about the two IRAK pseudokinases, IRAK2 and IRAK3. For this reason, I focused my research on the structural characterisation of the IRAK pseudokinases, to decode their roles within MyD88-dependent signalling. Chapter 3 is focused on the pseudokinase domains of IRAK2 and IRAK3. Chapter 4 describes the identification and characterisation of small molecules that bind to IRAK2 and IRAK3, to evaluate their potential as drug targets. Chapter 5 centres on the interaction of the E3 ubiquitin ligase TRAF6 with IRAK2 and IRAK3. I discovered that the kinase domain of IRAK2 is monomeric in solution, while IRAK3 is in a concentration-dependent equilibrium of monomeric and dimeric states. ATP-binding assays revealed that IRAK2 binds ATP in a Mg-dependent fashion, while IRAK3 does not bind ATP, but binds to the ATP-competitive pankinase inhibitor staurosporine. I present the first crystal structure of the pseudokinase domain of human IRAK3, which reveals a pseudoactive conformation and provides support for IRAK3's inability to bind ATP. IRAK3 forms a hexamer in the crystal via two alternating pseudosymmetric homo-dimer interfaces: The αC-to-αC interface, which centres on the αC-helices of IRAK3, and the αG-to-αG interface with central αG-helices. Structure-guided point-mutations in the αC-to-αC interface identified this interface to form the in-solution dimer of IRAK3. Interestingly, I found that a higher-order assembly of IRAK3 pseudokinase domains with alternating αC-to-αC and αG-to-αG interfaces would create a helical IRAK3 filament. Strikingly, I identified a third putative interface on the IRAK3 surface in a conservation analysis of vertebrate IRAK3 sequences. The third interface is centred around the αEF-helix of IRAK3 and closely resembles the αEF-to-αEF interface of the previously reported IRAK4 homo-dimer. Mutations in IRAK3 that are linked to the pathogenesis of early-onset persistent asthma lie in the putative αEF-interface of IRAK3. Intriguingly, the three distinct dimerisation interfaces on the surface of the pseudokinase domain of IRAK3 do not overlap and allowed for the creation of a higher-order assembly model of IRAK3 and IRAK4 molecules. The model resembles a potential IRAK3-mediated inhibited state of the IRAK4 kinase domain, which would be a novel mechanism of kinase inhibition by a pseudokinase oligomer. Furthermore, these findings demonstrate for the first time that the ability of the Myddosome to signal may be tightly regulated by oligomerisation of the kinase and pseudokinase domains of the IRAKs. In collaboration with Janssen Pharmaceutica, I performed a high-throughput assay to screen for small molecule binders of the IRAK pseudokinases. I identified and started to characterise twelve small molecules that bind to the IRAK3 pseudokinase domain with nanomolar affinities. Lastly, I detected a highly conserved helical region in the C-terminal domains of IRAK1 and IRAK2, which might be the first clue for a function of the C-terminal domains of the IRAKs in addition to their role in TRAF6-binding. I investigated the interaction between TRAF6 and the C-terminal domains of IRAKs and developed a high-throughput HTRF assay for the identification of protein-protein interaction inhibitors to disrupt this interaction. Together, this work lays the foundation to explore the IRAK pseudokinases as potential drug targets for the treatment of innate immune diseases in the future.
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- 2020
53. TRAF6 regulates autophagy and apoptosis of melanoma cells through c‐Jun/ATG16L2 signaling pathway
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Yeye Guo, Xu Zhang, Jie Li, Zhe Zhou, Susi Zhu, Waner Liu, Juan Su, Xiang Chen, and Cong Peng
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apoptosis ,ATG16L2 ,autophagy ,c‐Jun ,melanoma ,TRAF6 ,Medicine - Abstract
Abstract Autophagy and apoptosis are essential processes that participate in cell death and maintain cellular homeostasis. Dysregulation of these biological processes results in the development of diseases, including cancers. Therefore, targeting the interaction between apoptosis and autophagy offers a potential strategy for cancer therapy. Melanoma is the most lethal skin cancer. We previously found that tumor necrosis factor receptor‐associated factor 6 (TRAF6) is overexpressed in melanoma and benefits the malignant phenotype of melanoma cells. Additionally, TRAF6 promotes the activation of cancer‐associated fibroblasts in melanoma. However, the role of TRAF6 in autophagy and apoptosis remains unclear. In this study, we found that knockdown of TRAF6 induced both apoptosis and autophagy in melanoma cells. Transcriptomic data and real‐time PCR analysis demonstrated reduced expression of autophagy related 16 like 2 (ATG16L2) in TRAF6‐deficient melanoma cells. ATG16L2 knockdown resulted in increased autophagy and apoptosis. Mechanism studies confirmed that TRAF6 regulated ATG16L2 expression through c‐Jun. Importantly, targeting TRAF6 with cinchonine, a TRAF6 inhibitor, effectively suppressed the growth of melanoma cells by inducing autophagy and apoptosis through the TRAF6/c‐Jun/ATG16L2 signaling pathway. These findings highlight the pivotal role of TRAF6 in regulating autophagy and apoptosis in melanoma, emphasizing its significance as a novel therapeutic target for melanoma treatment.
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- 2023
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54. Regulation mechanisms of CARMA1–Bcl10–MALT1 complex assembly inferred from the analysis of TRAF6‐deficient cells.
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Inoue, Kentaro, Yasuda, Tomoharu, Baba, Yoshihiro, Yamamoto, Tadashi, Kurosaki, Tomohiro, and Shinohara, Hisaaki
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B cell receptors , *UBIQUITINATION , *CELL analysis , *B cells , *MATHEMATICAL analysis , *MATHEMATICAL models , *UBIQUITIN ligases - Abstract
The CARMA1–Bcl10–MALT1 (CBM) signalosome is a crucial module of NF‐κB activation in B cell receptor (BCR) signaling. Biophysical studies have shown that the E3 ubiquitin ligase TRAF6 cooperatively modifies the CBM signalosome; however, the specific details regarding how TRAF6 is involved in BCR signal‐induced CBM formation remain unclear. In this study, we aimed to reveal the influences of TRAF6 on CBM formation and TAK1 and IKK activities using DT40 B cells which lack all the exons of TRAF6. In TRAF6‐null cells we found: (i) attenuation of TAK1 activity and abolishment of IKK activity and (ii) sustained binding of CARMA1 to Bcl10. To account for the molecular mechanism causing these dynamics, we performed a mathematical model analysis. The mathematical model analysis showed that the regulation of IKK activation by TRAF6 can reproduce TAK1 and IKK activities in TRAF6 null cells, and that the TRAF6 related signal‐dependent inhibitor suppresses CARMA1 binding to Bcl10 in wild‐type cells. These results suggest that TRAF6 contributes to the positive regulation of IKK activation via TAK1, alongside the negative signal‐dependent regulation of CARMA1 binding to Bcl10. [ABSTRACT FROM AUTHOR]
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- 2023
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55. Hedgehog signaling regulates bone homeostasis through orchestrating osteoclast differentiation and osteoclast–osteoblast coupling.
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Lu, Weiguang, Zheng, Chao, Zhang, Hongyang, Cheng, Pengzhen, Miao, Sheng, Wang, Huanbo, He, Ting, Fan, Jing, Hu, Yaqian, Liu, He, Jia, Liyuan, Hao, Xue, Luo, Zhuojing, Xu, Jiake, Jie, Qiang, and Yang, Liu
- Abstract
Imbalance of bone homeostasis induces bone degenerative diseases such as osteoporosis. Hedgehog (Hh) signaling plays critical roles in regulating the development of limb and joint. However, its unique role in bone homeostasis remained largely unknown. Here, we found that canonical Hh signaling pathway was gradually augmented during osteoclast differentiation. Genetic inactivation of Hh signaling in osteoclasts, using Ctsk-Cre;Smof/f conditional knockout mice, disrupted both osteoclast formation and subsequent osteoclast–osteoblast coupling. Concordantly, either Hh signaling inhibitors or Smo/Gli2 knockdown stunted in vitro osteoclast formation. Mechanistically, Hh signaling positively regulated osteoclast differentiation via transactivation of Traf6 and stabilization of TRAF6 protein. Then, we identified connective tissue growth factor (CTGF) as an Hh-regulatory bone formation-stimulating factor derived from osteoclasts, whose loss played a causative role in osteopenia seen in CKO mice. In line with this, recombinant CTGF exerted mitigating effects against ovariectomy induced bone loss, supporting a potential extension of local rCTGF treatment to osteoporotic diseases. Collectively, our findings firstly demonstrate that Hh signaling, which dictates osteoclast differentiation and osteoclast–osteoblast coupling by regulating TRAF6 and CTGF, is crucial for maintaining bone homeostasis, shedding mechanistic and therapeutic insights into the realm of osteoporosis. [ABSTRACT FROM AUTHOR]
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- 2023
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56. MiR-146a-5p delivered by hucMSC extracellular vesicles modulates the inflammatory response to sulfur mustard-induced acute lung injury.
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Pei, Zhipeng, Cen, Jinfeng, Zhang, Xinkang, Gong, Chuchu, Sun, Mingxue, Meng, Wenqi, Mao, Guanchao, Wan, Jingjing, Hu, Bingyue, He, Xiaowen, Xu, Qingqiang, Han, Hua, and Xiao, Kai
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EXTRACELLULAR vesicles , *MUSTARD gas , *CHEMICAL warfare agents , *POISONS , *LUNG injuries , *INFLAMMATION - Abstract
Background: Sulfur mustard (SM) is a highly toxic chemical warfare agent that has caused numerous casualties during wars and conflicts in the past century. Specific antidotes or therapeutic strategies are rare due to the complicated mechanism of toxicity, which still awaits elucidation. Clinical data show that acute lung injury (ALI) is responsible for most mortality and morbidity after SM exposure. Extracellular vesicles are natural materials that participate in intercellular communication by delivering various substances and can be modified. In this study, we aim to show that extracellular vesicles derived from human umbilical cord mesenchymal stromal cells (hucMSC-EVs) could exert therapeutic effects on SM-induced ALI, and to explain the underlying mechanism of effects. Methods: MiR-146a-5p contained in hucMSC-EVs may be involved in the process of hucMSC-EVs modulating the inflammatory response to SM-induced ALI. We utilized miR-146a-5p delivered by extracellular vesicles and further modified hucMSCs with a miR-146a-5p mimic or inhibitor to collect miR-146a-5p-overexpressing extracellular vesicles (miR-146a-5p+-EVs) or miR-146a-5p-underexpressing extracellular vesicles (miR-146a-5p−-EVs), respectively. Through in vivo and in vitro experiments, we investigated the mechanism. Results: The effect of miR-146a-5p+-EVs on improving the inflammatory reaction tied to SM injury was better than that of hucMSC-EVs. We demonstrated that miR-146a-5p delivered by hucMSC-EVs targeted TRAF6 to negatively regulate inflammation in SM-induced ALI models in vitro and in vivo. Conclusion: In summary, miR-146a-5p delivered by hucMSC-EVs targeted TRAF6, causing hucMSC-EVs to exert anti-inflammatory effects in SM-induced ALI; thus, hucMSC-EVs treatment may be a promising clinical therapeutic after SM exposure. [ABSTRACT FROM AUTHOR]
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- 2023
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57. 2,3,4',5-Tetrahydroxystilbene-2-O-β-d-glucoside attenuates atherosclerotic progression by inhibiting inflammation via downregulation of TNF receptor-associated factor 6 expression.
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YE, Z.-R., LIN, Y.-J., CAI, Y.-N., HONG, N.-J., KANG, D.-L., LI, M., and ZENG, Z.-X.
- Abstract
OBJECTIVE: Atherosclerosis (As) is an inflammatory disease, and 2,3,4',5-tetrahydroxystilbene-2-O-ß-d-glucoside (TSG) has been shown to suppress inflammation. However, it is still unclear if TSG alleviates As by inhibiting inflammation. MATERIALS AND METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to assess the mRNA levels of tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6), TNF-a and interleukin-6 (IL-6) in lipoprotein E knockout (ApoE -/-) mice with As. Hematoxylin-eosin (H&E) staining was performed to examine the atherosclerotic plaques in the aortic sinus. QRT-PCR and western blotting were used to measure the expression levels of TRAF6, TNF-a, and IL-6 in human umbilical vein endothelial cells (HUVECs), and enzyme-linked immunosorbent assays (ELISAs) were performed to monitor the levels of TNF-a and IL-6 in serum and cell culture medium. RESULTS: TSG inhibited subendothelial plaques formation in the aortic sinus and inhibited the levels of total cholesterol (TCHO), low-density lipoprotein (LDL), TRAF6, TNF-a and IL-6 in AS mice in a dose-dependent manner. Moreover, TSG attenuated the oxidatively modified LDL (ox-LDL)-induced increases in TRAF6, TNF-a and IL-6 expression, whereas TRAF6 overexpression reversed the TSG-induced decreases in TRAF6, TNF-a, and IL-6 expression in HUVECs. CONCLUSIONS: TSG attenuates atherosclerotic progression by inhibiting inflammation via the downregulation of TRAF6 in ApoE-/- mice and HUVECs. [ABSTRACT FROM AUTHOR]
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- 2023
58. MIR222HG attenuates macrophage M2 polarization and allergic inflammation in allergic rhinitis by targeting the miR146a-5p/TRAF6/NF-kB axis .
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Silu Wen, Fen Li, Yulei Tang, Lin Dong, Yan He, Yuqin Deng, and Zezhang Tao
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ALLERGIC rhinitis ,LINCRNA ,MACROPHAGES ,AUTOREGRESSIVE models ,GENE expression - Abstract
Although M2 macrophages are involved in the orchestration of type 2 inflammation in allergic diseases, the mechanisms underlying non-coding RNA (ncRNA)-mediated macrophage polarization in allergic rhinitis (AR) have not been systematically understood. Here, we identified long non-coding RNA (lncRNA) MIR222HG as a key regulator of macrophage polarization and revealed its role in AR. Consistent with our bioinformatic analysis of GSE165934 dataset derived from the Gene Expression Omnibus (GEO) database, lncRNA-MIR222HG and murine mir222hg were downregulated in our clinical samples and animal models of AR, respectively. Mir222hg was upregulated in M1 macrophages and downregulated in M2 macrophages. The allergen-ovalbumin facilitated polarization of RAW264.7 cells to the M2 phenotype, accompanied by the downregulation of mir222hg expression in a dose-dependent manner. Mir222hg facilitates macrophage M1 polarization and reverses M2 polarization caused by ovalbumin. Furthermore, mir222hg attenuates macrophage M2 polarization and allergic inflammation in the AR mouse model. Mechanistically, a series of gain- and loss-of-function experiments and rescue experiments were performed to verify the role of mir222hg as a ceRNA sponge that adsorbed miR146a-5p, upregulated Traf6, and activated the IKK/IkB/P65 pathway. Collectively, the data highlight the remarkable role of MIR222HG in the modulation of macrophage polarization and allergic inflammation, as well as its potential role as a novel AR biomarker or therapeutic target. [ABSTRACT FROM AUTHOR]
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- 2023
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59. TRIM37 exacerbates hepatic ischemia/reperfusion injury by facilitating IKKγ translocation.
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Yang, Hang, Huang, Zuotian, Luo, Yunhai, Lei, Dengliang, Yan, Ping, Shen, Ai, Liu, Wenbin, Li, Dewei, and Wu, Zhongjun
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REPERFUSION , *REPERFUSION injury , *TUMOR necrosis factors , *ISCHEMIA , *PROTEIN expression - Abstract
Background: Hepatic ischemia/reperfusion (I/R) injury is one of the major pathological processes associated with various liver surgeries. However, there is still a lack of strategies to protect against hepatic I/R injury because of the unknown underlying mechanism. The present study aimed to identify a potential strategy and provide a fundamental experimental basis for treating hepatic I/R injury. Method: A classic 70% ischemia/reperfusion injury was established. Immunoprecipitation was used to identify direct interactions between proteins. The expression of proteins from different subcellular localizations was detected by Western blotting. Cell translocation was directly observed by immunofluorescence. HE, TUNEL and ELISA were performed for function tests. Result: We report that tripartite motif containing 37 (TRIM37) aggravates hepatic I/R injury through the reinforcement of IKK-induced inflammation following dual patterns. Mechanistically, TRIM37 directly interacts with tumor necrosis factor receptor-associated factor 6 (TRAF6), inducing K63 ubiquitination and eventually leading to the phosphorylation of IKKβ. TRIM37 enhances the translocation of IKKγ, a regulatory subunit of the IKK complex, from the nucleus to the cytoplasm, thereby stabilizing the cytoplasmic IKK complex and prolonging the duration of inflammation. Inhibition of IKK rescued the function of TRIM37 in vivo and in vitro. Conclusion: Collectively, the present study discloses some potential function of TRIM37 in hepatic I/R injury. Targeting TRIM37 might be potential for treatment against hepatic I/R injury.Targeting TRIM37 might be a potential treatment strategy against hepatic I/R injury. [ABSTRACT FROM AUTHOR]
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- 2023
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60. TRAF6 Promotes PRMT5 Activity in a Ubiquitination-Dependent Manner.
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Liu, Liu, Yin, Shasha, and Gan, Wenjian
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ARGININE metabolism , *IN vitro studies , *IMMUNOGLOBULINS , *IN vivo studies , *XENOGRAFTS , *ANALYSIS of variance , *CARCINOGENESIS , *COLONY-forming units assay , *ANIMAL experimentation , *PLASMIDS , *PRECIPITIN tests , *GENE expression , *IMMUNOBLOTTING , *CELL survival , *T-test (Statistics) , *CELL proliferation , *HISTONES , *METHYLATION , *DESCRIPTIVE statistics , *RESEARCH funding , *TUMOR markers , *CELL lines , *DATA analysis software , *MICE - Abstract
Simple Summary: PRMT5 is overexpressed and activated in various human cancers, including breast cancer. This study aims to dissect the mechanism underlying how PRMT5 is dysregulated in cancers. Our results demonstrate that TRAF6-mediated ubiquitination plays an important role in the regulation of PRMT5 activity and cell proliferation. Thus, inhibition of TRAF6 is a possible strategy for improving PRMT5 targeted therapy. Protein arginine methyltransferase 5 (PRMT5) is the primary enzyme generating symmetric dimethylarginine (sDMA) on numerous substrates, through which it regulates many cellular processes, such as transcription and DNA repair. Aberrant expression and activation of PRMT5 is frequently observed in various human cancers and associated with poor prognosis and survival. However, the regulatory mechanisms of PRMT5 remain poorly understood. Here, we report that TRAF6 serves as an upstream E3 ubiquitin ligase to promote PRMT5 ubiquitination and activation. We find that TRAF6 catalyzes K63-linked ubiquitination of PRMT5 and interacts with PRMT5 in a TRAF6-binding-motif-dependent manner. Moreover, we identify six lysine residues located at the N-terminus as the primarily ubiquitinated sites. Disruption of TRAF6-mediated ubiquitination decreases PRMT5 methyltransferase activity towards H4R3 in part by impairing PRMT5 interaction with its co-factor MEP50. As a result, mutating the TRAF6-binding motifs or the six lysine residues significantly suppresses cell proliferation and tumor growth. Lastly, we show that TRAF6 inhibitor enhances cellular sensitivity to PRMT5 inhibitor. Therefore, our study reveals a critical regulatory mechanism of PRMT5 in cancers. [ABSTRACT FROM AUTHOR]
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- 2023
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61. Circ_0004712 Silencing Suppresses the Aggressive Changes of Rheumatoid Arthritis Fibroblast-Like Synoviocytes by Targeting miR-633/TRAF6 Axis.
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Zhang, Shihui, Shen, Zhizhong, Chao, Gao, Du, Xiaolong, Zhang, Wentao, Jin, Dan, and Liu, Yafei
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CIRCULAR RNA , *RHEUMATOID arthritis , *GENE expression , *ENZYME-linked immunosorbent assay , *TUMOR necrosis factor receptors , *POLYMERASE chain reaction - Abstract
Circular RNA_0004712 (circ_0004712) is reported to be up-regulated in rheumatoid arthritis (RA) patients. Nevertheless, its role and mechanism in RA pathology remain to be clarified. RNA and protein expression was determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot assay. Cell viability, proliferation, apoptosis, migration, and inflammation were assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, 5-ethynyl-20-deoxyuridine assay, flow cytometry, scratch test, and enzyme-linked immunosorbent assay. The target correlation between microRNA-633 (miR-633) and circ_0004712 or TNF receptor associated factor 6 (TRAF6) was verified by dual-luciferase reporter assay and RNA immunoprecipitation assay. Circ_0004712 was up-regulated in RA synovial tissues and RA fibroblast-like synoviocytes (RA-FLSs). Circ_0004712 silencing suppressed the viability, proliferation, migration and inflammatory response and facilitated the apoptosis of RA-FLSs. miR-633 was confirmed to be a direct target of circ_0004712, and miR-633 knockdown reversed circ_0004712 silencing-mediated protective effects on the dysfunction and inflammation of RA-FLSs. TRAF6 was a direct target of miR-633, and miR-633 overexpression suppressed the aggressive changes of RA-FLSs by down-regulating TRAF6. Circ_0004712 could up-regulate TRAF6 expression by sponging miR-633 in RA-FLSs. Circ_0004712 interference inactivated nuclear factor (NF)-κB signaling by targeting miR-633/TRAF6 axis. Circ_0004712 silencing inhibited the aggressive changes of RA-FLSs by targeting miR-633/TRAF6 axis and NF-κB signaling, which provided new targets for RA therapy. [ABSTRACT FROM AUTHOR]
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- 2023
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62. circRASA2 靶向 miR-543/TRAF6 轴对 LPS 诱导的 牙周膜细胞增殖和成骨分化的影响.
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谢冰, 杨振宇, and 汤旭娜
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- 2023
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63. The Ubiquitin-Modifying Enzyme A20 Terminates C-Type Lectin Receptor Signals and Is a Suppressor of Host Defense against Systemic Fungal Infection.
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Liang, Jie, Zhang, Junyi J, Huang, Hsin-I, Kanayama, Masashi, Youssef, Nourhan, Jin, Yingai J, Reyes, Estefany Y, Abram, Clare L, Yang, Shigao, Lowell, Clifford A, Wang, Donghai, Shao, Ling, Shinohara, Mari L, Zhang, Jennifer Y, and Hammer, Gianna Elena
- Subjects
Vaccine Related ,Emerging Infectious Diseases ,Infectious Diseases ,Prevention ,Biodefense ,Aetiology ,2.1 Biological and endogenous factors ,Inflammatory and immune system ,Animals ,Bone Marrow Cells ,Candida albicans ,Candidiasis ,Dendritic Cells ,Female ,Fetus ,Host Microbial Interactions ,Immunity ,Innate ,Lectins ,C-Type ,Liver ,Male ,Mice ,Mice ,Inbred C57BL ,Mice ,Knockout ,Myeloid Differentiation Factor 88 ,NF-kappa B ,Primary Cell Culture ,Protein Processing ,Post-Translational ,Signal Transduction ,TNF Receptor-Associated Factor 6 ,Tumor Necrosis Factor alpha-Induced Protein 3 ,Ubiquitin ,Ubiquitination ,A20 ,C-type lectin receptors ,NE-kappa B ,TRAF6 ,cytokines ,dendritic cells ,fungal immunity ,innate immunity ,ubiquitination ,NF-κB ,Biological Sciences ,Agricultural and Veterinary Sciences ,Medical and Health Sciences ,Microbiology - Abstract
C-type lectin receptors (CLRs) play key roles in antifungal defense. CLR-induced NF-κB is central to CLR functions in immunity, and thus, molecules that control the amplitude of CLR-induced NF-κB could profoundly influence host defense against fungal pathogens. However, little is known about the mechanisms that negatively regulate CLR-induced NF-κB, and molecules which act on the CLR family broadly and which directly regulate acute CLR-signaling cascades remain unidentified. Here, we identify the ubiquitin-editing enzyme A20 as a negative regulator of acute NF-κB activation downstream of multiple CLR pathways. Absence of A20 suppression results in exaggerated CLR responses in cells which are A20 deficient and also cells which are A20 haplosufficient, including multiple primary immune cells. Loss of a single allele of A20 results in enhanced defense against systemic Candida albicans infection and prolonged host survival. Thus, A20 restricts CLR-induced innate immune responses in vivo and is a suppressor of host defense against systemic fungal infection.
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- 2020
64. Toxoplasma GRA15 limits parasite growth in IFNγ‐activated fibroblasts through TRAF ubiquitin ligases
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Mukhopadhyay, Debanjan, Sangaré, Lamba Omar, Braun, Laurence, Hakimi, Mohamed‐Ali, and Saeij, Jeroen PJ
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Biomedical and Clinical Sciences ,Emerging Infectious Diseases ,Biodefense ,Vaccine Related ,Infectious Diseases ,Prevention ,Foodborne Illness ,Aetiology ,2.1 Biological and endogenous factors ,Infection ,Inflammatory and immune system ,Animals ,Cells ,Cultured ,Fibroblasts ,Foreskin ,Gene Expression Regulation ,Humans ,Interferon-gamma ,Intracellular Signaling Peptides and Proteins ,Male ,Mice ,Protozoan Proteins ,Signal Transduction ,Toxoplasma ,Ubiquitin-Protein Ligases ,Vacuoles ,GRA15 ,IFN gamma ,p62 ,TRAF6 ,IFNγ ,Biological Sciences ,Information and Computing Sciences ,Medical and Health Sciences ,Developmental Biology ,Biological sciences ,Biomedical and clinical sciences - Abstract
The protozoan parasite Toxoplasma gondii lives inside a vacuole in the host cytosol where it is protected from host cytoplasmic innate immune responses. However, IFNγ-dependent cell-autonomous immunity can destroy the vacuole and the parasite inside. Toxoplasma strain differences in susceptibility to human IFNγ exist, but the Toxoplasma effector(s) that determine these differences are unknown. We show that in human primary fibroblasts, the polymorphic Toxoplasma-secreted effector GRA15 mediates the recruitment of ubiquitin ligases, including TRAF2 and TRAF6, to the vacuole membrane, which enhances recruitment of ubiquitin receptors (p62/NDP52) and ubiquitin-like molecules (LC3B, GABARAP). This ultimately leads to lysosomal degradation of the vacuole. In murine fibroblasts, GRA15-mediated TRAF6 recruitment mediates the recruitment of immunity-related GTPases and destruction of the vacuole. Thus, we have identified how the Toxoplasma effector GRA15 affects cell-autonomous immunity in human and murine cells.
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- 2020
65. TRAF6-Mediated Inflammatory Cytokines Secretion in LPS-induced Colorectal Cancer Cells Is Regulated by miR-140
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ZHU, GUANGWEI, LIN, CHUNLIN, CHENG, ZHIBIN, WANG, QIN, HOFFMAN, ROBERT M, SINGH, SHREE RAM, HUANG, YONGJIAN, ZHENG, WEI, YANG, SHUGANG, and YE, JIANXIN
- Subjects
Biotechnology ,Genetics ,Colo-Rectal Cancer ,Digestive Diseases ,Cancer ,2.1 Biological and endogenous factors ,Aetiology ,3' Untranslated Regions ,Apoptosis ,Biomarkers ,Tumor ,Case-Control Studies ,Cell Proliferation ,Colorectal Neoplasms ,Cytokines ,Gene Expression Regulation ,Neoplastic ,Humans ,Inflammation Mediators ,Intracellular Signaling Peptides and Proteins ,Lipopolysaccharides ,MicroRNAs ,Prognosis ,Signal Transduction ,Tumor Cells ,Cultured ,Colorectal cancer ,lipopolysaccharide ,miR-140 ,cytokines ,TRAF6 ,Immunology ,Oncology and Carcinogenesis ,Oncology & Carcinogenesis - Abstract
BACKGROUND/AIM:Colorectal cancer (CRC) cells secrete inflammatory cytokines that affect CRC progression. The aim of the present study was to determine if micro-RNA-140(miR-140) regulates inflammatory cytokine secretion induced by lipopolysaccharide (LPS) in colorectal cancer cells by targeting tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6). MATERIALS AND METHODS:Fifty fresh colon-cancer specimens and normal colorectal tissues were collected from patients with CRC and tested for the expression miR-140. Human CRC cell lines SW480 and HCT116 were treated with various concentrations and times with LPS. miR-140 and mRNA expression of potentially related genes were analyzed by qPCR. Protein expression was analyzed using western blot or ELISA. Overexpression plasmids with pcDNA3.1-TRAF6, pGL4.10-wtTRAF6 and pGL4.10-mutTRAF6 were constructed. miRNA target gene prediction and a dual luciferase assay were used to analyze miR-140-targeted TRAF6. RESULTS:miR-140 expression was up-regulated in CRC tissues. In CRC cells, LPS could increase miR-140 expression in a time- and concentration-dependent manner. LPS increased inflammatory cytokine mRNA expression levels in SW480 and HCT116 human colon-cancer cells. miRNA-140 suppressed TRAF6 expression via targeting the 3'UTR. TRAF6 affected miR-140-mediated inflammatory cytokine expression of SW480 and HCT116 cells under LPS treatment. CONCLUSION:miR-140 regulates inflammatory cytokine secretion of LPS-induced colorectal cancer cells by targeting TRAF6.
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- 2020
66. The YTHDF1–TRAF6 pathway regulates the neuroinflammatory response and contributes to morphine tolerance and hyperalgesia in the periaqueductal gray
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Handong Ouyang, Jianxing Zhang, Dongmei Chi, Kun Zhang, Yongtian Huang, Jingxiu Huang, Wan Huang, and Xiaohui Bai
- Subjects
YTHDF1 ,TRAF6 ,Morphine tolerance ,Morphine-induced hyperalgesia ,Inflammatory factors ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Long-term use of opioids such as morphine has negative side effects, such as morphine analgesic tolerance and morphine-induced hyperalgesia (MIH). These side effects limit the clinical use and analgesic efficacy of morphine. Elucidation of the mechanisms and identification of feasible and effective methods or treatment targets to solve this clinical phenomenon are important. Here, we discovered that YTHDF1 and TNF receptor-associated factor 6 (TRAF6) are crucial for morphine analgesic tolerance and MIH. The m6A reader YTHDF1 positively regulated the translation of TRAF6 mRNA, and chronic morphine treatments enhanced the m6A modification of TRAF6 mRNA. TRAF6 protein expression was drastically reduced by YTHDF1 knockdown, although TRAF6 mRNA levels were unaffected. By reducing inflammatory markers such as IL-1β, IL-6, TNF-α and NF-κB, targeted reduction of YTHDF1 or suppression of TRAF6 activity in ventrolateral periaqueductal gray (vlPAG) slows the development of morphine analgesic tolerance and MIH. Our findings provide new insights into the mechanism of morphine analgesic tolerance and MIH indicating that YTHDF1 regulates inflammatory factors such as IL-1β, IL-6, TNF-α and NF-κB by enhancing TRAF6 protein expression.
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- 2022
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67. The circular RNA circ_0099630/miR-940/receptor-associated factor 6 regulation cascade modulates the pathogenesis of periodontitis
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Xue-Qin Zhao, Chuan-Bei Ao, and Yi-Tong Yan
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Circ_0099630 ,MiR-940 ,Periodontitis ,TRAF6 ,Dentistry ,RK1-715 - Abstract
Background/purpose: Periodontitis is one of the highly prevalent chronic inflammatory conditions in adults. The importance of circular RNAs (circRNAs) in the regulation of inflammation has been gradually reported in recent years, but the role of circRNA circ_0099630 in periodontitis has not been reported. Materials and methods: The contents of circ_0099630, microRNA-940 (miR-940) and tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6) were measured using quantitative real-time polymerase chain reaction (qRT-PCR) or Western blot. Inflammatory factor secretion, cell proliferation, and apoptosis were analyzed under the application of Enzyme-linked immunosorbent assay (ELISA), Cell Counting Kit-8 (CCK8), 5-ethynyl-2′-deoxyuridine (EdU) and flow cytometry, respectively. The Western blot also analyzed the phosphorylation levels of RELA proto-oncogene (P65) and IkappaBalpha (IκBα), key molecules of the nuclear factor kappa-B (NF-κB) pathway. The relationship between miR-940 and circ_0099630 or TRAF6 was verified by luciferase reporter system and RNA immunoprecipitation (RIP) assay. Results: Higher abundance of circ_0099630 and TRAF6 and lower miR-940 expression were observed in periodontitis, and circ_0099630 knockdown attenuated the damage of human PDL cells (PDLCs) induced by lipopolysaccharides (LPS). The relationship between miR-940 and circ_0099630 or TRAF6 was evidenced, while miR-940 downregulation diminished the repair effect of si-circ_0099630 on overexpression LPS-induced damage in PDLCs. Similarly, TRAF6 upregulation impaired the mitigating effect of miR-940 overexpression on LPS-induced injury in PDLCs. Circ_0099630 silencing evidently curbed the phosphorylation levels of P65 and IκBα and thus attenuating the inflammatory response by acting on the miR-940/TRAF6 axis. Conclusion: Silencing circ_0099630 alleviates LPS-induced periodontal ligament cell injury via targeting miR-940/TRAF6/NF-κB in periodontitis.
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- 2022
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68. Akt: a key transducer in cancer
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Pei-Jane Tsai, Yi-Hsin Lai, Rajesh Kumar Manne, Yau-Sheng Tsai, Dos Sarbassov, and Hui-Kuan Lin
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PI3K ,Akt ,TRAF6 ,Skp2 ,Cancer ,Posttranslational modifications ,Medicine - Abstract
Abstract Growth factor signaling plays a pivotal role in diverse biological functions, such as cell growth, apoptosis, senescence, and migration and its deregulation has been linked to various human diseases. Akt kinase is a central player transmitting extracellular clues to various cellular compartments, in turn executing these biological processes. Since the discovery of Akt three decades ago, the tremendous progress towards identifying its upstream regulators and downstream effectors and its roles in cancer has been made, offering novel paradigms and therapeutic strategies for targeting human diseases and cancers with deregulated Akt activation. Unraveling the molecular mechanisms for Akt signaling networks paves the way for developing selective inhibitors targeting Akt and its signaling regulation for the management of human diseases including cancer.
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- 2022
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69. The OX40-TRAF6 axis promotes CTLA-4 degradation to augment antitumor CD8+ T-cell immunity
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Yu, Jizhang, Cui, Jikai, Zhang, Xi, Xu, Heng, Chen, Zhang, Li, Yuan, Niu, Yuqing, Wang, Song, Ran, Shuan, Zou, Yanqiang, Ye, Weicong, Zhang, Dan, Zhou, Cheng, Xia, Jiahong, and Wu, Jie
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- 2023
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70. miR-146-5p restrains calcification of vascular smooth muscle cells by suppressing TRAF6
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Yang Jing, Zhou Xiaoman, Lu Jingwei, and Li Meng
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mir-146-5p ,traf6 ,vascular calcification ,atherosclerosis ,Medicine - Abstract
Vascular calcification is a prominent manifestation of advanced atherosclerosis. Tumor necrosis factor-receptor-associated factors (TRAFs) were reported to participate in atherosclerosis development. In this study, the role and mechanism of TRAF6 in vascular calcification were explored. To induce the vascular calcification, oxidized low-density lipoprotein (Ox-LDL) was applied to treat vascular smooth muscle cells (VSMCs). TRAF6 protein expression in VSMCs was assessed by western blotting. Osteogenic differentiation of VSMCs was assessed by alkaline phosphatase activity analysis. Mineral deposition in VSMCs was evaluated by von Kossa staining. VSMC proliferation, migration, apoptosis, inflammation, and reactive oxygen species (ROS) generation were detected using cell counting kit-8, Transwell, flow cytometry, reverse transcriptase quantitative polymerase chain reaction (RT-qPCR), and dichlorodihydrofluorescein diacetate staining, respectively. Luciferase reporter assay was utilized to identify the binding relationship between miR-146-5p and TRAF6 in VSMCs. We found that Ox-LDL administration induced the calcification of VSMCs and elevated the TRAF6 level. TRAF6 knockdown restrained VSMC calcification, proliferation, migration, inflammation, and ROS generation caused by Ox-LDL. Mechanically, TRAF6 was targeted by miR-146-5p in VSMCs. Furthermore, TRAF6 overexpression offset the inhibitory effects of miR-146-5p upregulation on vascular calcification in VSMCs under the Ox-LDL condition. Overall, miR-146-5p restrains the calcification of VSMCs by suppressing TRAF6.
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- 2022
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71. Down-regulated miR-146a expression with increased neutrophil extracellular traps and apoptosis formation in autoimmune-mediated diffuse alveolar hemorrhage
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Yu-Tung Hsieh, Yu-Chi Chou, Pin-Yu Kuo, Hung-Wen Tsai, Yi-Ting Yen, Ai-Li Shiau, and Chrong-Reen Wang
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Diffuse alveolar hemorrhage ,MiR-146a ,TRAF6 ,Neutrophil extracellular trap ,NETosis ,Apoptosis ,Medicine - Abstract
Abstract Background Increasing evidences have suggested an important role of microRNAs (miRNAs) in regulating cell death processes including NETosis and apoptosis. Dysregulated expression of miRNAs and increased formation of neutrophil extracellular traps (NETs) and apoptosis participate in autoimmune-mediated diffuse alveolar hemorrhage (DAH), mostly associated with pulmonary capillaritis in systemic lupus erythematosus (SLE) patients. In particular, besides the inhibition of apoptosis, miR-146a can control innate and acquired immune responses, and regulate the toll-like receptor pathway through targeting TRAF6 to reduce the expression of pro-inflammatory cytokines/chemokines like IL-8, a NETosis inducer. Methods Expression of miR-146a, TRAF6 and NETs were examined in peripheral blood neutrophils (PBNs) and lung tissues from SLE-associated DAH patients, and in neutrophils and pristane-induced DAH lung tissues from C57BL/6 mice. To assess NETs formation, we examined NETosis-related DNAs morphology and crucial mediators including protein arginine deiminase 4 and citrullinated Histone 3. Expression of miR-146a and its endogenous RNA SNHG16 were studied in HL-60 promyelocytic cells and MLE-12 alveolar cells during NETosis and apoptosis processes, respectively. MiR-146a-overexpressed and CRISPR-Cas13d-mediated SNHG16-silenced HL-60 cells were investigated for NETosis. MiR-146a-overexpressed MLE-12 cells were analyzed for apoptosis. Pristane-injected mice received intra-pulmonary miR-146a delivery to evaluate therapeutic efficacy in DAH. Results In DAH patients, there were down-regulated miR-146a levels with increased TRAF6 expression and PMA/LPS-induced NETosis in PBNs, and down-regulated miR-146a levels with increased TRAF6, high-mobility group box 1 (HMGB1), IL-8, NETs and apoptosis expression in lung tissues. HMGB1-stimulated mouse neutrophils had down-regulated miR-146a levels with increased TRAF6, IL-8 and NETs expression. PMA-stimulated HL-60 cells had down-regulated miR-146a levels with enhanced NETosis. MiR-146a-overexpressed or SNHG16-silenced HL-60 cells showed reduced NETosis. Apoptotic MLE-12 cells had down-regulated miR-146a expression and increased HMGB1 release, while miR-146a-overexpressed MLE-12 cells showed reduced apoptosis and HMGB1 production. There were down-regulated miR-146a levels with increased TRAF6, HMGB1, IL-8, NETs and apoptosis expression in mouse DAH lung tissues. Intra-pulmonary miR-146a delivery could suppress DAH by reducing TRAF6, IL-8, NETs and apoptosis expression. Conclusions Our results demonstrate firstly down-regulated pulmonary miR-146a levels with increased TRAF6 and IL-8 expression and NETs and apoptosis formation in autoimmune-mediated DAH, and implicate a therapeutic potential of intra-pulmonary miR-146a delivery.
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- 2022
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72. Mechanisms of Neuropathic Pain and Pain-Relieving Effects of Exercise Therapy in a Rat Neuropathic Pain Model
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Sumizono M, Yoshizato Y, Yamamoto R, Imai T, Tani A, Nakanishi K, Nakakogawa T, Matsuoka T, Matsuzaki R, Tanaka T, and Sakakima H
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ccr2 ,hippocampal dentate gyrus ,microglia ,neurogenesis ,spinal dorsal horn ,traf6 ,Medicine (General) ,R5-920 - Abstract
Megumi Sumizono,1,2 Yushin Yoshizato,1 Ryohei Yamamoto,1 Takaki Imai,1 Akira Tani,2 Kazuki Nakanishi,2 Tomomi Nakakogawa,2 Teruki Matsuoka,2 Ryoma Matsuzaki,2 Takashi Tanaka,3 Harutoshi Sakakima2 1Department of Rehabilitation, Kyushu University of Nursing and Social Welfare, Kumamoto, Japan; 2Department of Physical Therapy, School of Health Sciences, Faculty of Medicine, Kagoshima University, Kagoshima, Japan; 3Department of Rehabilitation, Kumamoto Health Science University, Kumamoto, JapanCorrespondence: Megumi Sumizono, Department of Rehabilitation, Kyushu University of Nursing and Social Welfare, 888 Tominoo, Tamana, Kumamoto, 865-0062, Japan, Tel/Fax +81 968-75-1931, Email sumizono@kyushu-ns.ac.jpPurpose: Pain disrupts the daily and social lives of patients with neuropathic pain. Effective treatment of neuropathic pain is difficult. Pharmacological treatments for neuropathic pain are limited, and 40– 60% of patients do not achieve even partial relief of their pain. This study created a chronic constriction injury (CCI) model in rats to examine the effects of regular exercise on neuropathic pain relief, elucidate the mechanism, and determine the effects of neuropathic pain in the hippocampus.Methods: CCI model rats were randomly divided into exercise (Ex) and no exercise (No-Ex) groups. Normal rats (Normal group) were used as controls. The Ex group exercised on a treadmill at 20 m/min for 30 min, 5 days per week for 5 weeks post-CCI. The 50% pain response threshold was assessed by mechanical stimulation. Using immunohistochemistry, we examined activation of glial cells (microglia and astrocytes) by CCR2 and TRAF6 expression in the spinal cord dorsal horn and DCX and PROX1 expression in the hippocampal dentate gyrus.Results: The 50% pain response threshold was significantly lower in the Ex than in the No-Ex group at 5 weeks post-CCI, indicating pain relief. In the spinal cord dorsal horn, IBA1, CCR2, and TRAF6 expression was markedly lower in the Ex group than in the No-Ex group at 3 weeks post-CCI. IBA1, GFAP, CCR2, and TRAF6 expression was markedly lower in the Ex group than in the No-Ex group at 5 weeks post-CCI. In the hippocampus, DCX, but not PROX1, expression was significantly higher in the Ex group than in the No-Ex group at 3 weeks post-CCI. At 5 weeks post-CCI, both DCX and PROX1 expression was markedly increased in the Ex group compared to the No-Ex group.Conclusion: Our findings suggest that regular exercise can improve the neuropathic pain-induced neurogenic dysfunction in the hippocampal dentate gyrus.Keywords: CCR2, hippocampal dentate gyrus, microglia, neurogenesis, spinal dorsal horn, TRAF6
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- 2022
73. PF127 Hydrogel-Based Delivery of Exosomal CTNNB1 from Mesenchymal Stem Cells Induces Osteogenic Differentiation during the Repair of Alveolar Bone Defects.
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He, Longlong, Zhou, Qin, Zhang, Hengwei, Zhao, Ningbo, and Liao, Lifan
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BONE regeneration , *ALVEOLAR process , *BONE growth , *EXOSOMES , *MESENCHYMAL stem cells , *EXTRACELLULAR matrix , *BONE marrow , *BONE cells - Abstract
Pluronic F127 (PF127) hydrogel has been highlighted as a promising biomaterial for bone regeneration, but the specific molecular mechanism remains largely unknown. Herein, we addressed this issue in a temperature-responsive PF127 hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs)-derived exosomes (Exos) (PF127 hydrogel@BMSC-Exos) during alveolar bone regeneration. Genes enriched in BMSC-Exos and upregulated during the osteogenic differentiation of BMSCs and their downstream regulators were predicted by bioinformatics analyses. CTNNB1 was predicted to be the key gene of BMSC-Exos in the osteogenic differentiation of BMSCs, during which miR-146a-5p, IRAK1, and TRAF6 might be the downstream factors. Osteogenic differentiation was induced in BMSCs, in which ectopic expression of CTNNB1 was introduced and from which Exos were isolated. The CTNNB1-enriched PF127 hydrogel@BMSC-Exos were constructed and implanted into in vivo rat models of alveolar bone defects. In vitro experiment data showed that PF127 hydrogel@BMSC-Exos efficiently delivered CTNNB1 to BMSCs, which subsequently promoted the osteogenic differentiation of BMSCs, as evidenced by enhanced ALP staining intensity and activity, extracellular matrix mineralization (p < 0.05), and upregulated RUNX2 and OCN expression (p < 0.05). Functional experiments were conducted to examine the relationships among CTNNB1, microRNA (miR)-146a-5p, and IRAK1 and TRAF6. Mechanistically, CTNNB1 activated miR-146a-5p transcription to downregulate IRAK1 and TRAF6 (p < 0.05), which induced the osteogenic differentiation of BMSCs and facilitated alveolar bone regeneration in rats (increased new bone formation and elevated BV/TV ratio and BMD, all with p < 0.05). Collectively, CTNNB1-containing PF127 hydrogel@BMSC-Exos promote the osteogenic differentiation of BMSCs by regulating the miR-146a-5p/IRAK1/TRAF6 axis, thus inducing the repair of alveolar bone defects in rats. [ABSTRACT FROM AUTHOR]
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- 2023
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74. Circ_0003575 knockdown alleviates ox-LDL-induced human aortic endothelial cell dysfunction in atherosclerosis by miR-637/TRAF6 axis.
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Zhang, Zhanshuai, Qin, Shaoqiang, Wang, Rui, Fang, Zhiqin, Wang, Yaling, and Li, Fangjiang
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ENDOTHELIAL cells , *ENDOTHELIUM diseases , *AORTA , *ATHEROSCLEROSIS , *CIRCULAR RNA - Abstract
BACKGROUND: Circular RNAs (circRNAs) are involved in the progression of atherosclerosis (AS). The present study aimed to determine the functions and mechanism of circ_0003575 in AS. METHODS: Oxidized low-density lipoprotein (ox-LDL) was used to induce human aortic endothelial cells (HAECs) to establish an AS cell model. Cell Counting Kit-8 (CCK-8) assay and 5'-ethynyl-2'-deoxyuridine (EdU) assay were conducted to assess cell proliferation. Flow cytometry analysis was utilized to quantify cell apoptosis. Tube formation assay was performed to analyze angiogenesis ability. Enzyme linked immunosorbent assay (ELISA) was used to examine the concentrations of inflammatory factors. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blot were manipulated for the expression of circ_0003575, microRNA-637 (miR-637) and TNF receptor associated factor 6 (TRAF6). Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were adopted to estimate the downstream targets of circ_0003575. RESULTS: Ox-LDL treatment repressed the proliferation and angiogenesis and promoted the apoptosis and inflammation in HAECs. Circ_0003575 knockdown ameliorated ox-LDL-induced injury of HAECs. Circ_0003575 interacted with mi-R-637, which directly targeted TRAF6. Inhibition of miR-637 reversed the impacts of circ_0003575 knockdown on HAEC injury. Moreover, miR-637 overexpression promoted cell proliferation and angiogenesis and inhibited cell apoptosis and inflammation by targeting TRAF6 in ox-LDL-treated HAECs. Further, circ_0003575 silencing inhibited the activation of NF-κB pathway. CONCLUSION: Circ_0003575 knockdown alleviated ox-LDL-induced HAEC damage by regulating miR-637/TRAF6 and NF-κB pathways. [ABSTRACT FROM AUTHOR]
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- 2023
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75. Circ_0007706 downregulation ameliorates neonatal hypoxic ischemic encephalopathy via regulating the miR-579-3p/TRAF6 axis.
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Wang, Jinguang, Hua, Minmin, Li, Huixin, Xu, Dan, Li, Fangfang, and Xu, Falin
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CEREBRAL anoxia-ischemia , *DOWNREGULATION , *NEONATAL death , *BAX protein , *PROTEIN expression - Abstract
Neonatal hypoxic ischemic encephalopathy (HIE) is a main factor of neonatal death and permanent neurologic deficit. This study sought to investigate the functional role of hsa_circ_0007706 (circ_0007706) in modulating neonatal HIE. In vitro HIE cell model was established in hBMVECs under the condition of oxygen‑glucose deprivation/reperfusion (OGD/R) treatment. qRT-PCR analysis was utilized for detecting the level of circ_0007706, microRNA-579–3p (miR-579–3p) and TNF receptor-associated factor 6 (TRAF6). RNase R treatment and Oligo (dT) 18 primers were employed to verify the features of circ_0007706, and nucleocytoplasmic separation was conducted for determining the location of circ_0007706. CCK-8 assay, EdU assay, and flow cytometry were carried out to measure cell proliferation and apoptosis, respectively. The protein expression of Bax, Bcl-2 and TRAF6 was detected using western blot. Meanwhile, the levels of the pro-inflammatory factors were determined via ELISA. SOD activity and MDA level were assessed via the respective kits. Besides, dual-luciferase reporter assay and RNA pull-down were used to identify the association between miR-579–3p and circ_0007706 or TRAF6. Circ_0007706 was elevated in HIE newborns and OGD/R cell model. Knockdown of circ_0007706 greatly alleviated OGD/R-induced injury, inflammatory response and oxidative stress. We found that miR-579–3p was a direct target of circ_0007706, and miR-579–3p inhibitor could reverse the impact of circ_0007706 knockdown on OGD/R-caused cell damage in hBMVECs. In addition, miR-579–3p directly interacted with TRAF6, and the protective effects of miR-579–3p on OGD/R-induced injury in hBMVECs were harbored by TRAF6 overexpression. Our data indicated that circ_0007706 knockdown could downregulate the expression of TRAF6 by sponging miR-579–3p in OGD/R-treated hBMVECs. This study demonstrated that circ_0007706 knockdown assuaged HIE-induced injury by decreasing TRAF6 expression via targeting miR-579–3p. • Circ_0007706 was upregulated in HIE newborns and OGD/R-induced hBMVECs. • Circ_0007706 downregulation alleviated OGD/R-induced damage in hBMVECs. • Circ_0007706 downregulation mitigated OGD/R-induced damage via targeting miR-579–3p to regulate TRAF6 expression in hBMVECs. [ABSTRACT FROM AUTHOR]
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- 2023
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76. Different degree of loss‐of‐function among four missense mutations in the EDAR gene responsible for autosomal recessive hypohidrotic ectodermal dysplasia may be associated with the phenotypic severity.
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Yagi, Sasagu, Yasuno, Shuichiro, Ansai, Osamu, Hayashi, Ryota, and Shimomura, Yutaka
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Hypohidrotic ectodermal dysplasia is a rare condition characterized by hypohidrosis, hypodontia, and hypotrichosis. The disease can show X‐linked recessive, autosomal dominant or autosomal recessive inheritance trait. Of these, the autosomal forms are caused by mutations in either EDAR or EDARADD. To date, the underlying pathomechanisms or genotype–phenotype correlations for autosomal forms have not completely been disclosed. In this study, we performed a series of in vitro studies for four missense mutations in the death domain of EDAR protein: p.R358Q, p.G382S, p.I388T, and p.T403M. The results revealed that p.R358Q‐ and p.T403M‐mutant EDAR showed different expression patterns from wild‐type EDAR in both western blots and immunostainings. NF‐κB reporter assays demonstrated that all the mutant EDAR showed reduced activation of NF‐κB, but the reduction by p.G382S‐ and p.I388T‐mutant EDAR was moderate. Co‐immunoprecipitation assays showed that p.R358Q‐ and p.T403M‐mutant EDAR did not bind with EDARADD at all, whereas p.G382S‐ and p.I388T‐mutant EDAR maintained the affinity to some extent. Furthermore, we demonstrated that all the mutant EDAR proteins analyzed aberrantly bound with TRAF6. Sum of the data suggest that the degree of loss‐of‐function is different among the mutant EDAR proteins, which may be associated with the severity of the disease. [ABSTRACT FROM AUTHOR]
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- 2023
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77. TLR4、TRAF6、IL-6和IL-1β在深靜脈血栓大鼠模型中的表達和意義.
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王喜尧, 王育文, 齐桂云, 徐洪伟, and 杜桂芹
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VENOUS thrombosis , *PEARSON correlation (Statistics) , *TOLL-like receptors , *THROMBOSIS , *INTERLEUKIN-6 - Abstract
Objective: To investigate the effects of four inflammatory factors (Toll-like receptor 4, TLR4; TNF receptor-related factor 6, TRAF6; interleukin-6, IL-6; interleukin-1β, IL-1β) in rats with deep vein thrombosis expression and role in deep vein thrombosis. Methods: SD rats were randomly divided into control group (n=10) and model group (n=90), and the deep vein thrombosis model was established by vascular clamp combined with immobilization. After modeling, the rats were sacrificed at 1 h, 6 h, 12 h, 1 d, 3 d,7 d, 14 d, 21 d, and 28 d, respectively. The thrombus weight/length ratio and the concentration of 4 inflammatory factors in plasma were detected, and histological observation was performed. Results: (1) The thrombus weight/length ratio at 1 h, 6 h, and 12 h was lower, and there was no significant difference between the groups (P>0.05); the thrombus weight/length ratio at 1 d, 3 d, and 7 d remained at a high level, and there was no significant difference between the groups. The thrombus weight/length ratio decreased on 14 d, 21 d, and 28 d, and there was no significant difference between groups (P>0.05). (2) The thrombus weight/length ratios in the model group from 1 to 7days were significantly higher than those in the normal group (P<0.05). (3) The concentrations of TLR4, TRAF6, IL-6 and IL-1β in the model group from 1 to 7 days were significantly higher than those in the normal group (P<0.05). (4) Pearson correlation analysis showed that the concentrations of TLR4, TRAF6, IL-6 and IL-1β were positively correlated with the degree of thrombosis. Conclusion: Up-regulation of TLR4, TRAF6, IL-6 and IL-1β in rats with deep vein thrombosis may play an important role in deep vein thrombosis. [ABSTRACT FROM AUTHOR]
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- 2023
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78. miRNA-Induced Downregulation of IPMK in Macrophages Mediates Lipopolysaccharide-Triggered TLR4 Signaling.
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Lee, Haein, Kim, Eunha, and Kim, Seyun
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TUMOR necrosis factors , *TOLL-like receptors , *GENE transfection , *DOWNREGULATION , *BINDING sites , *UBIQUITINATION - Abstract
Inositol polyphosphate multikinase (IPMK) is a pleiotropic enzyme responsible for the production of inositol polyphosphates and phosphoinositide. IPMK in macrophages was identified as a key factor for the full activation of the Toll-like receptor 4 (TLR4) signaling pathway and inflammation by directly interacting with tumor necrosis factor receptor-associated factor 6 (TRAF6). Here, dynamic changes of IPMK levels in lipopolysaccharide (LPS)-stimulated macrophages and their functional significance were investigated. Both the mRNA and protein levels of IPMK were acutely decreased in mouse and human macrophages when cells were stimulated with LPS for between 1 and 6 h. Analysis of the 3' untranslated region (UTR) of mouse IPMK mRNA revealed a highly conserved binding site for miR-181c. Transfection of miR-181c mimics into RAW 264.7 macrophages led to decreased IPMK 3'UTR-luciferase reporter activity and lowered endogenous IPMK levels. When the genomic deletion of a 33-bp fragment containing a putative miR-181c-binding site was introduced within the IPMK 3'UTR of RAW 264.7 macrophages (264.7Δ3′UTR), LPS-triggered downregulation of IPMK levels was prevented. LPS treatment in 264.7Δ3′UTR macrophages decreased TLR4-induced signaling and the expression of proinflammatory cytokines. In response to LPS stimulation, K63-linked ubiquitination of TRAF6 was impaired in 264.7Δ3′UTR macrophages, suggesting an action of IPMK in the suppression of TRAF6 activation. Therefore, our findings reveal that LPS-mediated suppression of IPMK regulates the full activation of TLR4 signaling and inflammation in macrophages. [ABSTRACT FROM AUTHOR]
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- 2023
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79. TRAF6 controls T cell homeostasis by maintaining the equilibrium of MALT1 scaffolding and protease functions.
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O'Neill, Thomas J., Gewies, Andreas, Seeholzer, Thomas, and Krappmann, Daniel
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T cells ,REGULATORY T cells ,T cell receptors ,HOMEOSTASIS - Abstract
MALT1 is a core component of the CARD11-BCL10-MALT1 (CBM) signalosome, in which it acts as a scaffold and a protease to bridge T cell receptor (TCR) ligation to immune activation. As a scaffold, MALT1 binds to TRAF6, and T cell-specific TRAF6 ablation or destruction of MALT1-TRAF6 interaction provokes activation of conventional T (Tconv) effector cells. In contrast, MALT1 protease activity controls the development and suppressive function of regulatory T (Treg) cells in a T cell-intrinsic manner. Thus, complete loss of TRAF6 or selective inactivation of MALT1 catalytic function in mice skews the immune system towards autoimmune inflammation, but distinct mechanisms are responsible for these immune disorders. Here we demonstrate that TRAF6 deletion or MALT1 paracaspase inactivation are highly interdependent in causing the distinct immune pathologies. We crossed mice with T cell-specific TRAF6 ablation (Traf6-ΔT) and mice with a mutation rendering the MALT1 paracaspase dead in T cells (Malt1 PD-T) to yield Traf6-ΔT;Malt1 PD-T double mutant mice. These mice reveal that the autoimmune inflammation caused by TRAF6-ablation relies strictly on the function of the MALT1 protease to drive the activation of Tconv cells. Vice versa, despite the complete loss of Treg cells in Traf6-ΔT;Malt1 PD-T double mutant mice, inactivation of the MALT1 protease is unable to cause autoinflammation, because the Tconv effector cells are not activated in the absence of TRAF6. Consequentially, combined MALT1 paracaspase inactivation and TRAF6 deficiency in T cells mirrors the immunodeficiency seen upon T cell-specific MALT1 ablation. [ABSTRACT FROM AUTHOR]
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- 2023
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80. Bovine Lactoferrin Suppresses Tumor Angiogenesis through NF-κB Pathway Inhibition by Binding to TRAF6.
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Ayuningtyas, Nurina Febriyanti, Chea, Chanbora, Ando, Toshinori, Saninggar, Karina Erda, Tanimoto, Keiji, Inubushi, Toshihiro, Maishi, Nako, Hida, Kyoko, Shindoh, Masanobu, Miyauchi, Mutsumi, and Takata, Takashi
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LACTOFERRIN , *VASCULAR endothelial growth factors , *VASCULAR endothelial growth factor receptors , *NEOVASCULARIZATION - Abstract
Tumor angiogenesis is essential for tumor progression. The inhibition of tumor angiogenesis is a promising therapy for tumors. Bovine lactoferrin (bLF) has been reported as an anti-tumor agent. However, bLF effects on tumor angiogenesis are not well demonstrated. This study evaluated the inhibitory effects of bLF on tumor angiogenesis in vivo and in vitro. Herein, tumor endothelial cells (TECs) and normal endothelial cells (NECs) were used. Proliferation, migration, tube formation assays, RT-PCR, flow cytometry, Western blotting, siRNA experiments and immunoprecipitation were conducted to clarify the mechanisms of bLF-induced effects. CD-31 immunoexpression was examined in tumor tissues of oral squamous cell carcinoma mouse models with or without Liposomal bLF (LbLF)-administration. We confirmed that bLF inhibited proliferation/migration/tube formation and increased apoptosis in TECs but not NECs. TNF receptor-associated factor 6 (TRAF6), p-p65, hypoxia inducible factor-α (HIF-1α) and vascular endothelial growth factor (VEGF) were highly expressed in TECs. In TECs, bLF markedly downregulated VEGF-A, VEGF receptor (VEGFR) and HIF-1α via the inhibition of p-p65 through binding with TRAF6. Since NECs slightly expressed p-p65, bLF–TRAF-6 binding could not induce detectable changes. Moreover, orally administrated LbLF decreased CD31-positive microvascular density only in TECs. Hence, bLF specifically suppressed tumor angiogenesis through p-p65 inhibition by binding to TRAF6 and suppressing HIF-1α activation followed by VEGF/VEGFR down-regulation. Collectively, bLF can be an anti-angiogenic agent for tumors. [ABSTRACT FROM AUTHOR]
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- 2023
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81. MiR-124 Reduced Neuroinflammation after Traumatic Brain Injury by Inhibiting TRAF6.
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Yang, Yongxiang, Ye, Yuqin, Fan, Kexia, Luo, Jianing, Yang, Yongjian, and Ma, Yuan
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Introduction: Neuroinflammation contributes to secondary injury after traumatic brain injury (TBI), which has been mainly mediated by the microglia. MiR-124 was reported to play an important role in the polarization of microglia by targeting TLR4 signaling pathway. However, the role and mechanism of miR-124 in neuroinflammation mediated by microglia after TBI is unclear. To clarify this, we performed this research. Methods: The expression of miR-124 was first measured by RT-PCR in the injured brain at 1/3/7 days post-TBI. Then, miR-124 mimics or inhibitors administration was used to interfere the expression of miR-124 at 24 h post-TBI. Subsequently, the microglia polarization markers were detected by RT-PCR, the expression of inflammatory cytokines was detected by ELISA, the expression of TLR4/MyD88/IRAK1/TRAF6/NF-κB was measured by WB, and the neurological deficit was evaluated by NSS and MWM test. At last, in vitro experiments were performed to explore the exact target molecule of miR-124 on TLR4 signaling pathway. Results: Animal research indicated that the expression of miR-124 was downregulated after TBI. Upregulation of miR-124 promoted the M2 polarization of microglia and inhibited the activity of TLR4 pathway, as well as reduced neuroinflammation and neurological deficit after TBI. In vitro experiments indicated that miR-124 promoted the M2 polarization of microglia and reduced neuroinflammation by inhibiting TRAF6. Conclusion: This study demonstrated that upregulation of miR-124 promoted the M2 polarization of microglia and reduced neuroinflammation after TBI by inhibiting TRAF6. [ABSTRACT FROM AUTHOR]
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- 2023
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82. MIR222HG attenuates macrophage M2 polarization and allergic inflammation in allergic rhinitis by targeting the miR146a-5p/TRAF6/NF-κB axis
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Silu Wen, Fen Li, Yulei Tang, Lin Dong, Yan He, Yuqin Deng, and Zezhang Tao
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allergic rhinitis ,long non-coding RNAs ,miR146a-5p ,TRAF6 ,macrophages polarization ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Although M2 macrophages are involved in the orchestration of type 2 inflammation in allergic diseases, the mechanisms underlying non-coding RNA (ncRNA)-mediated macrophage polarization in allergic rhinitis (AR) have not been systematically understood. Here, we identified long non-coding RNA (lncRNA) MIR222HG as a key regulator of macrophage polarization and revealed its role in AR. Consistent with our bioinformatic analysis of GSE165934 dataset derived from the Gene Expression Omnibus (GEO) database, lncRNA-MIR222HG and murine mir222hg were downregulated in our clinical samples and animal models of AR, respectively. Mir222hg was upregulated in M1 macrophages and downregulated in M2 macrophages. The allergen-ovalbumin facilitated polarization of RAW264.7 cells to the M2 phenotype, accompanied by the downregulation of mir222hg expression in a dose-dependent manner. Mir222hg facilitates macrophage M1 polarization and reverses M2 polarization caused by ovalbumin. Furthermore, mir222hg attenuates macrophage M2 polarization and allergic inflammation in the AR mouse model. Mechanistically, a series of gain- and loss-of-function experiments and rescue experiments were performed to verify the role of mir222hg as a ceRNA sponge that adsorbed miR146a-5p, upregulated Traf6, and activated the IKK/IκB/P65 pathway. Collectively, the data highlight the remarkable role of MIR222HG in the modulation of macrophage polarization and allergic inflammation, as well as its potential role as a novel AR biomarker or therapeutic target.
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- 2023
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83. Structural Characterization of TRAF6 N-Terminal for Therapeutic Uses and Computational Studies on New Derivatives
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Omur Guven, Belgin Sever, Faika Başoğlu-Ünal, Abdulilah Ece, Hiroshi Tateishi, Ryoko Koga, Mohamed O. Radwan, Nefise Demir, Mustafa Can, Mutlu Dilsiz Aytemir, Jun-ichiro Inoue, Masami Otsuka, Mikako Fujita, Halilibrahim Ciftci, and Hasan DeMirci
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TRAF6 ,zinc finger ,RING domain ,cancer ,X-ray crystallography ,structural biology ,Medicine ,Pharmacy and materia medica ,RS1-441 - Abstract
Tumor necrosis factor receptor-associated factors (TRAFs) are a protein family with a wide variety of roles and binding partners. Among them, TRAF6, a ubiquitin ligase, possesses unique receptor binding specificity and shows diverse functions in immune system regulation, cellular signaling, central nervous system, and tumor formation. TRAF6 consists of an N-terminal Really Interesting New Gene (RING) domain, multiple zinc fingers, and a C-terminal TRAF domain. TRAF6 is an important therapeutic target for various disorders and structural studies of this protein are crucial for the development of next-generation therapeutics. Here, we presented a TRAF6 N-terminal structure determined at the Turkish light source “Turkish DeLight” to be 3.2 Å resolution at cryogenic temperature (PDB ID: 8HZ2). This structure offers insight into the domain organization and zinc-binding, which are critical for protein function. Since the RING domain and the zinc fingers are key targets for TRAF6 therapeutics, structural insights are crucial for future research. Separately, we rationally designed numerous new compounds and performed molecular docking studies using this template (PDB ID:8HZ2). According to the results, 10 new compounds formed key interactions with essential residues and zinc ion in the N-terminal region of TRAF6. Molecular dynamic (MD) simulations were performed for 300 ns to evaluate the stability of three docked complexes (compounds 256, 322, and 489). Compounds 256 and 489 was found to possess favorable bindings with TRAF6. These new compounds also showed moderate to good pharmacokinetic profiles, making them potential future drug candidates as TRAF6 inhibitors.
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- 2023
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84. Toxoplasma GRA15 Activates the NF-κB Pathway through Interactions with TNF Receptor-Associated Factors
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Sangaré, Lamba Omar, Yang, Ninghan, Konstantinou, Eleni K, Lu, Diana, Mukhopadhyay, Debanjan, Young, Lucy H, and Saeij, Jeroen PJ
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Biochemistry and Cell Biology ,Biological Sciences ,Biodefense ,Emerging Infectious Diseases ,Infectious Diseases ,2.1 Biological and endogenous factors ,Inflammatory and immune system ,Binding Sites ,Gene Expression ,Host-Pathogen Interactions ,Humans ,NF-kappa B ,Protein Binding ,Protozoan Proteins ,Signal Transduction ,Toxoplasma ,Toxoplasmosis ,Tumor Necrosis Factor Receptor-Associated Peptides and Proteins ,NF-kappa B pathway ,TRAF2 ,TRAF6 ,Toxoplasma gondii ,host-pathogen interactions ,NF-κB pathway ,Microbiology ,Biochemistry and cell biology ,Medical microbiology - Abstract
The protozoan parasite Toxoplasma gondii secretes proteins from specialized organelles, the rhoptries, and dense granules, which are involved in the modulation of host cell processes. Dense granule protein GRA15 activates the nuclear factor kappa B (NF-κB) pathway, which plays an important role in cell death, innate immunity, and inflammation. Exactly how GRA15 activates the NF-κB pathway is unknown. Here we show that GRA15 interacts with tumor necrosis factor receptor-associated factors (TRAFs), which are adaptor proteins functioning upstream of the NF-κB transcription factor. We identified several TRAF binding sites in the GRA15 amino acid sequence and showed that these are involved in NF-κB activation. Furthermore, a TRAF2 knockout cell line has impaired GRA15-mediated NF-κB activation. Thus, we determined the mechanism for GRA15-dependent NF-κB activation.IMPORTANCE The parasite Toxoplasma can cause birth defects and severe disease in immunosuppressed patients. Strain differences in pathogenicity exist, and these differences are due to polymorphic effector proteins that Toxoplasma secretes into the host cell to coopt host cell functions. The effector protein GRA15 of some Toxoplasma strains activates the nuclear factor kappa B (NF-κB) pathway, which plays an important role in cell death, innate immunity, and inflammation. We show that GRA15 interacts with TNF receptor-associated factors (TRAFs), which are adaptor proteins functioning upstream of the NF-κB transcription factor. Deletion of TRAF-binding sites in GRA15 greatly reduces its ability to activate the NF-κB pathway, and TRAF2 knockout cells have impaired GRA15-mediated NF-κB activation. Thus, we determined the mechanism for GRA15-dependent NF-κB activation.
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- 2019
85. MicroRNA 322-5p reduced neuronal inflammation via the TLR4/TRAF6/NF-κB axis in a rat epilepsy model
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Zhou Qin, Wang Qiong, He Baomei, Kong Haibo, Luo Huanjun, Wang Xiaowei, and Wang Wenlan
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epilepsy ,inflammation ,mir-322-5p ,traf6 ,nf-κb ,apoptosis ,Medicine - Abstract
This study aimed to determine whether microRNA-322-5p regulates seizure and seizure damage by targeting the TLR4/TRAF6/NF-κB-associated inflammatory signaling pathway. In a pilocarpine-induced epileptic rat model, the expressions of miR-322-5p, TLR4, NF-κB, TRAF6, IRF5, IL-1β, and GABA were assessed by a quantitative polymerase chain reaction and western blotting. Tunel detects hippocampal neuron apoptosis. The results showed that the expression of miR-322-5p significantly decreased in status epilepticus (SE) rats. The reduction of miR-322-5p was accompanied by increased levels of pro-inflammatory cytokines, an increased NF-κB expression, and reduced γ-aminobutyric acid (GABA) levels. Exogenous miR-322-5p reduced the expression of inflammatory molecules and increased the GABA levels in SE rats, and also reduced hippocampal neuronal cell apoptosis caused by epilepsy. In conclusion, the miR-322-5p significantly inhibited the TLR4/TRAF6/NF-κB-associated inflammation and reduced neuronal apoptosis, suggesting that its induction may be of potential interest for novel antiseizure medications.
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- 2022
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86. Exosomal miR-125a-5p regulates T lymphocyte subsets to promote silica-induced pulmonary fibrosis by targeting TRAF6
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Mingcui Ding, Yangqing Pei, Chengpeng Zhang, Yuanmeng Qi, Jiarui Xia, Changfu Hao, and Wu Yao
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Exosomes ,MiR-125a-5p ,Pulmonary fibrosis ,TRAF6 ,T lymphocyte ,Environmental pollution ,TD172-193.5 ,Environmental sciences ,GE1-350 - Abstract
Silicosis caused by long-term inhalation of crystalline silica during occupational activities seriously threatens the health of occupational populations. Imbalances in T helper 1(Th1), Th2, Th17, and regulatory T cells (Tregs) promote the development of pulmonary silicosis. Exosomes and their contents, especially microRNAs (miRNAs), represent a new type of intercellular signal transmission mediator related to various diseases including pulmonary fibrosis. However, whether exosomal miRNAs can affect the progression of silicosis by regulating T cell differentiation remains to be determined. To test this hypothesis, we established a miR-125a-5p antagomir mouse model and examined changes in miR-125a-5p levels and T cell subtypes. We found that miR-125a-5p levels were increased in lung tissues and serum exosomes in the silica group at 7 days and 28 days. Downregulation of miR-125a-5p attenuated α-smooth muscle actin (α-SMA), collagen I, fibronectin, p-p65, and p-inhibitor of nuclear factor kappa B (NF-κB) kinase (IKK) protein expression, while tumor necrosis factor receptor-associated factor 6 (TRAF6) and p-inhibitor of κBα (IKBα) expression were increased. MiR-125a-5p anta-miR treatment contributes to the maintenance of Th1/Th2 balance during the progression of pulmonary fibrosis. Our findings indicated that knockdown miR-125a-5p could regulate T lymphocyte subsets and significantly reduce pulmonary fibrosis by targeting TRAF6.
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- 2023
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87. A tetravalent peptide that binds to the RANK-binding region of TRAF6 via a multivalent interaction efficiently inhibits osteoclast differentiation.
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Anzai, Masataka, Watanabe-Takahashi, Miho, Kawabata, Hiroshi, Mizuno, Saori, Taguchi, Yuu, Inoue, Jun-ichiro, and Nishikawa, Kiyotaka
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OSTEOCLASTS , *OSTEOCLASTOGENESIS , *PEPTIDES , *NF-kappa B , *TRANCE protein , *BONE marrow cells , *TUMOR necrosis factors - Abstract
Inhibition of osteoclast differentiation is a promising approach for the treatment of osteoporosis and rheumatoid arthritis. Receptor activator of nuclear factor kappa B (NF-κB) (RANK), which is an essential molecule for osteoclast differentiation, interacts with tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6) to transduce downstream signals. Both RANK and TRAF6 have homo-trimeric structures, forming a multivalent interaction between the Pro-X-Glu-X-X-(aromatic/acidic) motif of RANK and the C-terminal domain of TRAF6 (TRAF-C), that markedly increases the binding affinity. Here, we designed a tetravalent peptide, RANK-tet, containing the TRAF-C-binding motif of RANK and found that RANK-tet binds to TRAF-C with high affinity. In contrast, a monomeric form of RANK-tet (RANK-mono) with the same TRAF-C-binding motif did not bind to TRAF-C, clearly indicating the multivalent interaction is strictly required for the high-affinity binding to TRAF-C. RANK-tet did not bind to a series of TRAF-C-mutants with an amino acid substitution in the RANK-binding region, indicating that RANK-tet specifically targets the RANK-binding region of TRAF-C. A cell-permeable form of RANK-tet that has poly-Arg residues at each C-terminal of the TRAF-C-binding motif efficiently inhibited the RANK ligand (RANKL)-induced differentiation of bone marrow cells to osteoclasts. Thus, this compound can be an effective anti-osteoclastogenic agent. • RANK-tet is a tetravalent peptide containing the TRAF-C-binding motif of RANK. • RANK-tet, but not its monomeric form, binds to TRAF-C with high affinity. • RANK-tet functions through a multivalent interaction to bind to TRAF-C. • RANK-tet specifically targets the RANK-binding region of TRAF-C. • A cell-permeable form of RANK-tet efficiently inhibited osteoclast differentiation. [ABSTRACT FROM AUTHOR]
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- 2022
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88. The YTHDF1–TRAF6 pathway regulates the neuroinflammatory response and contributes to morphine tolerance and hyperalgesia in the periaqueductal gray.
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Ouyang, Handong, Zhang, Jianxing, Chi, Dongmei, Zhang, Kun, Huang, Yongtian, Huang, Jingxiu, Huang, Wan, and Bai, Xiaohui
- Abstract
Long-term use of opioids such as morphine has negative side effects, such as morphine analgesic tolerance and morphine-induced hyperalgesia (MIH). These side effects limit the clinical use and analgesic efficacy of morphine. Elucidation of the mechanisms and identification of feasible and effective methods or treatment targets to solve this clinical phenomenon are important. Here, we discovered that YTHDF1 and TNF receptor-associated factor 6 (TRAF6) are crucial for morphine analgesic tolerance and MIH. The m6A reader YTHDF1 positively regulated the translation of TRAF6 mRNA, and chronic morphine treatments enhanced the m6A modification of TRAF6 mRNA. TRAF6 protein expression was drastically reduced by YTHDF1 knockdown, although TRAF6 mRNA levels were unaffected. By reducing inflammatory markers such as IL-1β, IL-6, TNF-α and NF-κB, targeted reduction of YTHDF1 or suppression of TRAF6 activity in ventrolateral periaqueductal gray (vlPAG) slows the development of morphine analgesic tolerance and MIH. Our findings provide new insights into the mechanism of morphine analgesic tolerance and MIH indicating that YTHDF1 regulates inflammatory factors such as IL-1β, IL-6, TNF-α and NF-κB by enhancing TRAF6 protein expression. [ABSTRACT FROM AUTHOR]
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- 2022
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89. HNE Induces the Hyperexpression of MUC5AC in Chronic Rhinosinusitis With Nasal Polyps by Activating the TRAF6/Autophagy Regulatory Axis.
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Zhang, Ying, Qi, Jing, Yan, Danqing, Deng, Yangquan, Zhang, Jian, and Luo, Qing
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NASAL polyps ,LEUCOCYTE elastase ,SINUSITIS ,AUTOPHAGY ,PROTEIN expression - Abstract
Background: Hypersecretion of mucin 5AC (MUC5AC) is a prominent feature of chronic rhinosinusitis with nasal polyps (CRSwNP) and autophagy plays a pivotal role in this process. TNF receptor-associated factor 6 (TRAF6) functions as a signal transducer in many inflammation diseases, whereas the correlation between TRAF6 and autophagy in CRSwNP remains unclear. Objective: To investigate the role of TRAF6 in the human neutrophil elastase (HNE)-induced autophagy and mucin MUC5AC over-expression in CRSwNP. Methods: Tissue specimens were obtained from control subjects and patients with CRSwNP. The relationships between HNE, TRAF6, autophagy, and MUC5AC were investigated. The effect of TRAF6 on HNE-mediated autophagy and hypersecretion of MUC5AC was assessed by in-vitro culture of HNECs treated with human recombinant HNE. Results: Patients with CRSwNP had more protein expression of HNE, MUC5AC, TRAF6, and light chain (LC3B), and increased levels of Beclin-1(BECN1) and autophagy-related gene 5 (ATG5) in mRNA level. Treatment of nasal epithelial cells with recombinant HNE induced the upregulation of TRAF6, autophagy, and MUC5AC. Alternatively, si-TRAF6 or autophagy inhibitor treatment mitigates the hyperexpression of MUC5AC before incubating with recombinant HNE. Conclusion: HNE promotes autophagy through TRAF6, resulting in hyperexpression of MUC5AC in CRSwNP. [ABSTRACT FROM AUTHOR]
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- 2022
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90. Molecular determinants of TRAF6 binding specificity suggest that native interaction partners are not optimized for affinity.
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Halpin, Jackson C., Whitney, Dustin, Rigoldi, Federica, Sivaraman, Venkat, Singer, Avinoam, and Keating, Amy E.
- Abstract
TRAF6 is an adaptor protein involved in signaling pathways that are essential for development and the immune system. It participates in many protein–protein interactions, some of which are mediated by the C‐terminal MATH domain, which binds to short peptide segments containing the motif PxExx[FYWHDE], where x is any amino acid. Blocking MATH domain interactions is associated with favorable effects in various disease models. To better define TRAF6 MATH domain binding preferences, we screened a combinatorial library using bacterial cell‐surface peptide display. We identified 236 of the best TRAF6‐interacting peptides and a set of 1,200 peptides that match the sequence PxE but do not bind TRAF6 MATH. The peptides that were most enriched in the screen bound TRAF6 tighter than previously measured native peptides. To better understand the structural basis for TRAF6 interaction preferences, we built all‐atom structural models of the MATH domain in complex with high‐affinity binders and nonbinders identified in the screen. We identified favorable interactions for motif features in binders as well as negative design elements distributed across the motif that can disfavor or preclude binding. Searching the human proteome revealed that the most biologically relevant TRAF6 motif matches occupy a different sequence space from the best hits discovered in combinatorial library screening, suggesting that native interactions are not optimized for affinity. Our experimentally determined binding preferences and structural models support the design of peptide‐based interaction inhibitors with higher affinities than endogenous TRAF6 ligands. [ABSTRACT FROM AUTHOR]
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- 2022
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91. A20 Enhances the Expression of the Proto-Oncogene C-Myc by Downregulating TRAF6 Ubiquitination after ALV-A Infection.
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Chen, Xueyang, Wang, Xingming, Yang, Yuxin, Fang, Chun, Liu, Jing, Liang, Xiongyan, and Yang, Yuying
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- *
AVIAN leukosis , *STUNTED growth , *HAIRPIN (Genetics) , *CELLULAR signal transduction , *PLASMIDS , *TUMOR growth , *UBIQUITINATION - Abstract
Hens infected with avian leukosis virus subgroup A (ALV-A) experience stunted growth, immunosuppression, and potentially, lymphoma development. According to past research, A20 can both promote and inhibit tumor growth. In this study, DF-1 cells were infected with ALV-A rHB2015012, and Gp85 expression was measured at various time points. A recombinant plasmid encoding the chicken A20 gene and short hairpin RNA targeting chicken A20 (A20-shRNA) was constructed and transfected into DF-1 cells to determine the effect on ALV-A replication. The potential signaling pathways of A20 were explored using bioinformatics prediction, co-immunoprecipitation, and other techniques. The results demonstrate that A20 and ALV-A promoted each other after ALV-A infection of DF-1 cells, upregulated A20, inhibited TRAF6 ubiquitination, and promoted STAT3 phosphorylation. The phosphorylated-STAT3 (p-STAT3) promoted the expression of proto-oncogene c-myc, which may lead to tumorigenesis. This study will help to further understand the tumorigenic process of ALV-A and provide a reference for preventing and controlling ALV. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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92. Akt: a key transducer in cancer.
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Tsai, Pei-Jane, Lai, Yi-Hsin, Manne, Rajesh Kumar, Tsai, Yau-Sheng, Sarbassov, Dos, and Lin, Hui-Kuan
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TRANSDUCERS , *DISEASE management , *CELL growth , *POST-translational modification - Abstract
Growth factor signaling plays a pivotal role in diverse biological functions, such as cell growth, apoptosis, senescence, and migration and its deregulation has been linked to various human diseases. Akt kinase is a central player transmitting extracellular clues to various cellular compartments, in turn executing these biological processes. Since the discovery of Akt three decades ago, the tremendous progress towards identifying its upstream regulators and downstream effectors and its roles in cancer has been made, offering novel paradigms and therapeutic strategies for targeting human diseases and cancers with deregulated Akt activation. Unraveling the molecular mechanisms for Akt signaling networks paves the way for developing selective inhibitors targeting Akt and its signaling regulation for the management of human diseases including cancer. [ABSTRACT FROM AUTHOR]
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- 2022
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93. The circular RNA circ_0099630/miR-940/receptor-associated factor 6 regulation cascade modulates the pathogenesis of periodontitis.
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Zhao, Xue-Qin, Ao, Chuan-Bei, and Yan, Yi-Tong
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CIRCULAR RNA ,PERIODONTITIS ,ENZYME-linked immunosorbent assay ,TUMOR necrosis factors ,PERIODONTAL ligament ,POLYMERASE chain reaction - Abstract
Periodontitis is one of the highly prevalent chronic inflammatory conditions in adults. The importance of circular RNAs (circRNAs) in the regulation of inflammation has been gradually reported in recent years, but the role of circRNA circ_0099630 in periodontitis has not been reported. The contents of circ_0099630, microRNA-940 (miR-940) and tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6) were measured using quantitative real-time polymerase chain reaction (qRT-PCR) or Western blot. Inflammatory factor secretion, cell proliferation, and apoptosis were analyzed under the application of Enzyme-linked immunosorbent assay (ELISA), Cell Counting Kit-8 (CCK8), 5-ethynyl-2′-deoxyuridine (EdU) and flow cytometry, respectively. The Western blot also analyzed the phosphorylation levels of RELA proto-oncogene (P65) and IkappaBalpha (IκBα), key molecules of the nuclear factor kappa-B (NF-κB) pathway. The relationship between miR-940 and circ_0099630 or TRAF6 was verified by luciferase reporter system and RNA immunoprecipitation (RIP) assay. Higher abundance of circ_0099630 and TRAF6 and lower miR-940 expression were observed in periodontitis, and circ_0099630 knockdown attenuated the damage of human PDL cells (PDLCs) induced by lipopolysaccharides (LPS). The relationship between miR-940 and circ_0099630 or TRAF6 was evidenced, while miR-940 downregulation diminished the repair effect of si-circ_0099630 on overexpression LPS-induced damage in PDLCs. Similarly, TRAF6 upregulation impaired the mitigating effect of miR-940 overexpression on LPS-induced injury in PDLCs. Circ_0099630 silencing evidently curbed the phosphorylation levels of P65 and IκBα and thus attenuating the inflammatory response by acting on the miR-940/TRAF6 axis. Silencing circ_0099630 alleviates LPS-induced periodontal ligament cell injury via targeting miR-940/TRAF6/NF-κB in periodontitis. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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94. MiR-146a-5p Promotes Dental Stem Cells Osteo/odontogenic Differentiation through NF-Kappa B Signaling Pathway by Targeting TRAF6.
- Author
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Xin YU, Jian Feng LU, Mei Qin GAO, Bin XIONG, and Wen Qian XIA
- Abstract
Objective: To screen miRNAs that could simultaneously regulate osteo/odontogenic differentiation of multiple stem cells, including dental pulp stem cells (DPSCs), stem cells from the apical papilla (SCAPs) and periodontal ligament stem cells (PDLSCs). Methods: Differentially expressed miRNAs analysis on three miRNA microarrays data of dental stem cells undergoing osteo/odontogenic differentiation (GSE138180, GSE154466 and GSE159508) was performed, and miR-146a-5p were identified by bioinformatic prediction, dual-luciferase reporter assay and quantitative real-time polymerase chain reaction (PCR). In addition, differentially expressed genes between miR-146a-5p overexpressed group and control group (GSE79341) were applied for KEGG pathways enrichment analysis. Results: MiR-146a-5p expression increased in the osteo/odontogenic differentiation of DPSCs, SCAPs and PDLSCs. Tumour necrosis factor receptor (TNFR)-associated factor 6 (TRAF6) was identified as the target gene of miR-146a-5p. Furthermore, miR-146a-5p could influence the NF-Kappa B signalling pathway. Conclusion: This study suggests that miR-146a-5p could promote differentiation in multiple dental stem cells through the NF-Kappa B signalling pathway by targeting TRAF6. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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95. Resveratrol inhibits TRAF6/PTCH/SMO signal and regulates prostate cancer progression.
- Author
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Li, Jianping, Wang, Ziming, Li, Hecheng, Cao, Jun, Nan, Ning, Zhai, Xiaoqiang, Liu, Ying, and Chong, Tie
- Abstract
Prostate cancer (PC) is one of the most common types of cancers among men, referring to the uncontrolled growth of the prostate gland. It is increasingly recognized that the interaction of the glioma-associated oncogene (GLI) pathway and androgen receptor affects PC progression. Nevertheless, the effects of resveratrol on PC progression via Hedgehog (HH) signaling remain unexplored. In this study, the castration-sensitive and castration-resistant xenograft models in mice are systematically established using two different PC cell lines (LNCaP and PC-3). Further, the Western blotting, immunohistochemistry, MTT, Transwell, and RT-qPCR analyses are performed to verify the mechanistic views of resveratrol on PC and HH signals in vitro and in vivo. Resveratrol showed epithelial–mesenchymal transition (EMT) progression, inhibiting the tumor size and expression levels of vimentin, vascular endothelial growth factor (VEGF), and matrix metalloproteinases (MMP) 7, as well as upregulating the expression profiles the E-cadherin and Annexin 2. Moreover, resveratrol inhibited the hedgehog (HH) signals and tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6) levels exhibiting the therapeutic action on castration-sensitive and castration-resistant PC cell lines. In summary, the overexpression of TRAF6 enhanced the viability and EMT progression of cancer cells. The resveratrol could alleviate the TRAF6 effect and regulate the HH signal to affect PC progression. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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96. MicroRNA miR-24-3p Mediates the Negative Regulation of Lipopolysaccharide-Induced Endometrial Inflammatory Response by Targeting TNF Receptor-Associated Factor 6 (TRAF6)
- Author
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Oladejo AO, Li Y, Imam BH, Ma X, Shen W, Wu X, Jiang W, Yang J, Lv Y, Ding X, Wang S, and Yan Z
- Subjects
endometritis ,mir-24-3p ,traf6 ,nf-ĸb/mapk ,inflammation ,Pathology ,RB1-214 ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Ayodele Olaolu Oladejo,1,2 Yajuan Li,1 Bereket Habte Imam,1,3 Xiaoyu Ma,1 Wenxiang Shen,1 Xiaohu Wu,1 Wei Jiang,1 Jie Yang,1 Yanan Lv,1 Xuezhi Ding,1 Shengyi Wang,1 Zuoting Yan1 1Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Science, Lanzhou, 730050, People’s Republic of China; 2Department of Animal Health Technology, Oyo State College of Agriculture and Technology, Igboora, 201103, Nigeria; 3Department of Veterinary Science, Hamelmalo Agricultural College, Keren, 397, EritreaCorrespondence: Zuoting YanKey Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Science, Lanzhou, 730050, People’s Republic of China, Tel +8613919067215, Email yanzuoting@caas.cnPurpose: Endometritis is a female reproductive disease that affects the cattle industries development and microRNAs (miRNAs) play a pivotal role and critical regulators of the innate immune response in varieties of diseases. The present study intends to investigate the regulatory role of miR‐24-3p in the innate immune response involved in endometritis and evaluate its therapeutic potential.Methods: Whole mice uteri and bovine endometrial epithelial cells (BEECs) were separately stimulated with LPS. The BEECs were also transfected with miR-24-3p mimic and negative control; siTRAF6 and siNC; pcDNA3.1 empty and pcDNA3.1(+)TRAF6 separately with LPS stimulation. The expression levels of miR‐24-3p and TRAF6 were measured via quantitative real‐time polymerase chain reaction (qRT-PCR) and Western blot, respectively. LPS‐induced inflammatory response assessed by inflammatory cytokines secretion and expression via ELISA and qRT-PCR. Bioinformatics analysis and luciferase reporter assay validated the interaction between miR‐24-3p and TRAF6. The activation of the NF‐ĸB/MAPK pathway and p65 phosphorylation was investigated by Western blot and immunofluorescence assay, respectively.Results: The expression of miR‐24-3p was decreased, and TRAF6 was elevated with an increased level of pro-inflammatory cytokines in LPS‐treated BEECs and mice uterus. The overexpression of miR‐24-3p suppressed LPS‐induced secretion of inflammatory cytokines (IL‐1β, IL‐6, IL-8 and TNF-α) and deactivation of NF‐ĸB/MAPK pathways. The downregulation of TRAF6 inhibited LPS‐induced inflammatory response in BEECs. TRAF6 is validated as a target of miR‐24-3p, and miR‐24-3p reversed the overexpression of cloned TRAF6 on inflammation response in BEECs.Conclusion: Our findings demonstrate that the overexpression of miR‐24-3p attenuates endometrial inflammation and the expression of pro-inflammatory mediators via suppressing TRAF6. Therefore, modulating the pathogenesis of endometritis and possibly, a therapeutic potential against endometritis.Keywords: endometritis, miR-24-3p, TRAF6, NF-ĸB/MAPK, inflammation
- Published
- 2022
97. Circ_0114427 promotes LPS-induced septic acute kidney injury by modulating miR-495-3p/TRAF6 through the NF-κB pathway
- Author
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Lei Xu, Hongxia Cao, Peng Xu, Mingxi Nie, and Chun Zhao
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circ_0114427 ,mir-495-3p ,traf6 ,aki ,inflammatory response ,nf-κb/p65 pathway ,Internal medicine ,RC31-1245 - Abstract
Backgrounds Septic acute kidney injury (AKI) is a severe illness in clinics. Enriching researches investigated the regulatory network of AKI during the past decades, evidences showed that circular RNAs (circRNAs) were involved in the molecular mechanism of human AKI. However, the special responses remain largely elusive. Thus, the study aims to investigate the function of circ_0114427 in the progression of AKI. Methods The levels of circ_0114427, miR-495-3p and Tumour Necrosis Factor Receptor-Associated Factor 6 (TRAF6) were both assessed by quantitative real-time polymerase chain reaction (qRT-PCR). In addition, lipopolysaccharide (LPS) was applied to establish AKI cell model, and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay was carried out to determine the viability of LPS-induced HK-2 cells. The expression of TRAF6, B-cell lymphoma-2 (Bcl-2), Bcl2-associated X (Bax), cleave-caspase 3, caspase 3, total IκBα (t-IκBα), phospho-IκBα (p-IκBα), total p65 (t-p65) and phospho-p65 (p-p65) were all detected via western blot. The levels of IL-1β and TNF-α were identified by western blot and ELISA. What’s more, cell apoptosis was measured by flow cytometry. Lastly, dual-luciferase reporter, RNA Immunoprecipitation (RIP) and RNA pull-down assays were employed to verify the relationships between miR-495-3p and circ_0114427 or TRAF6 in vitro. Results The level of miR-495-3p was remarkably restrained while circ_0114427 and TRAF6 levels were specially reinforced in AKI patient serum samples and LPS-induced HK-2 cells. Moreover, IL-1β and TNF-α were highly expressed in LPS-induced AKI cells. Functionally, circ_0114427 was a sponge of miR-495-3p, and circ_0114427 silence-mediated effects in LPS-induced HK-2 cells were partly ameliorated by the addition of miR-495-3p inhibitor. Moreover, TRAF6 was a target gene of miR-495-3p, and the inhibiting effect of miR-495-3p on cell apoptosis and inflammatory response was mitigated by TRAF6 overexpression. Mechanistically, the circ_0114427/miR-495-3p/TRAF6 axis modulated cell apoptosis and inflammatory response via NF-κB/p65 signalling pathway in AKI. Conclusion Circ_0114427 regulated cell apoptosis and inflammatory response through miR-495-3p/TRAF6 axis via NF-κB/p65 signalling pathway, providing a novel mechanism in clinical treatment of AKI patients.Highlights Circ_0114427 is upregulated in serum specimens from septic AKI patients and LPS-induced HK-2 cells. LPS treatment suppresses cell viability and promotes apoptosis and inflammation in HK-2 cells. Circ_0114427 knockdown ameliorates the effects of LPS on cell viability, apoptosis and inflammation in HK-2 cells. Circ_0114427 regulates LPS-induced HK-2 cell injury by regulating miR-495-3p/TRAF6/NF-κB/p65 axis.
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- 2022
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98. Cyclo(L-Pro-L-Trp) from Chilobrachys jingzhao alleviates formalin-induced inflammatory pain by suppressing the inflammatory response and inhibiting TRAF6-mediated MAPK and NF-κB signaling pathways.
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Liu, Xin-Yue, Huang, Jin-Chang, Zhang, Tao, Wang, Han-Rui, Xu, Qi-Hui, Xia, Yu-Gui, Xu, A-Jing, Yang, Ze-Yong, Sun, Lei, Zhao, Wen-Juan, Zhao, Jun, Qian, Feng, and Hou, Ai-Jun
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MITOGEN-activated protein kinases , *NF-kappa B , *NITRIC-oxide synthases , *TUMOR necrosis factors , *DRUG interactions - Abstract
[Display omitted] • A cyclic dipeptide was first isolated from Chilobrachys jingzhao. • It remarkably alleviated formalin-induced inflammatory pain. • It significantly inhibited the inflammatory responses in vivo and in vitro. • It suppressed the ubiquitination of TRAF6 and activation of MAPK and NF-κB signaling pathways. Chronic pain has emerged as a significant public health issue, seriously affecting patients' quality of life and psychological well-being, with a lack of effective pharmacological treatments. Numerous studies have indicated that macrophages play a crucial role in inflammatory pain, and targeting neuro-immune interactions for drug development may represent a promising direction for pain management. Chilobrachys jingzhao (C. jingzhao) is used as a folk medicine of the Li nationality with the efficacy of eliminating swelling, detoxicating, and relieving pain, and the related products are widely used in the market. However, the chemical constituents of C. jingzhao have not been reported, and the pharmacodynamic substance and the precise functional mechanism are unrevealed. Here we isolated a cyclic dipeptide, cyclo(L-Pro-L-Trp) (CPT) from C. jingzhao for the first time. CPT remarkably alleviated formalin-induced inflammatory pain and significantly inhibited inflammatory responses. In vivo , CPT attenuated neutrophil infiltration and plantar tissue edema and suppressed the mRNA expression of pro-inflammatory molecules. In vitro , CPT suppressed inflammation triggered by lipopolysaccharide (LPS) in both RAW 264.7 and iBMDM cells, reducing expressions of inducible nitric oxide synthase (iNOS), superoxide, and pro-inflammatory molecules. A mechanistic study revealed that CPT exerted an anti-inflammatory activity by blocking the mitogen-activated protein kinases (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathways, as well as alleviating the ubiquitination of tumor necrosis factor receptor-associated factor 6 (TRAF6). Our results elucidated the pharmacodynamic material basis of C. jingzhao , and CPT can be a promising lead for alleviating inflammation and inflammatory pain. [ABSTRACT FROM AUTHOR]
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- 2024
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99. Active fraction of Polyrhachis vicina (Rogers) inhibits osteoclastogenesis by targeting Trim38 mediated proteasomal degradation of TRAF6.
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Feng, Xiaoliang, Wei, Guining, Su, Yuangang, Xian, Yansi, Liu, Zhijuan, Gao, Yijie, Liang, Jiamin, Lian, Haoyu, Xu, Jiake, Zhao, Jinmin, Liu, Qian, and Song, Fangming
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Reactive Oxygen Species (ROS) is a key factor in the pathogenesis of osteoporosis (OP) primarily characterized by excessive osteoclast activity. Active fraction of Polyrhachis vicina Rogers (AFPR) exerts antioxidant effects and possesses extensive promising therapeutic effects in various conditions, however, its function in osteoclastogenesis and OP is unknown. The aim of this study is to elucidate the cellular and molecular mechanisms of AFPR in OP. CCK8 assay was used to evaluate the cell viability under AFPR treatment. TRAcP staining, podosome belts staining and bone resorption were used to test the effect of AFPR on osteoclastogenesis. Immunofluorescence staining was used to observe the effect of AFPR on ROS production. si-RNA transfection, coimmunoprecipitation and Western-blot were used to clarify the underlying mechanisms. Further, an ovariectomy (OVX) -induced OP mice model was used to identify the effect of AFPR on bone loss using Micro-CT scanning and histological examination. In the present study, AFPR inhibited osteoclast differentiation and bone resorption induced by nuclear factor-κB receptor activator (NF-κB) ligand (RANKL) in dose-/ time-dependent with no cytotoxicity. Meanwhile, AFPR decreased RANKL-mediated ROS levels and enhanced ROS scavenging enzymes. Mechanistically, AFPR promoted proteasomal degradation of TRAF6 by significantly upregulating its K48-linked ubiquitination, subsequently inhibiting NFATc1 activity. We further observed that tripartite motif protein 38 (TRIM38) could mediate the ubiquitination of TRAF6 in response to RANKL. Moreover, TRIM38 could negatively regulate the RANKL pathway by binding to TRAF6 and promoting K48-linked polyubiquitination. In addition, TRIM38 deficiency rescued the inhibition of AFPR on ROS and NFATc1 activity and osteoclastogenesis. In line with these results, AFPR reduced OP caused by OVX through ameliorating osteoclastogenesis. AFPR alleviates ovariectomized-induced bone loss via suppressing ROS and NFATc1 by targeting Trim38 mediated proteasomal degradation of TRAF6. The research offers innovative perspectives on AFPR's suppressive impact in vivo OVX mouse model and in vitro , and clarifies the fundamental mechanism. Polyrhachis vicina is a species of ant insects (also called black ant) (Wei et al., 2023 ; Zhang et al., 2022). AFPR is the components of active fraction of Polyrchachis vicina Rogers. As shown in the figure, AFPR alleviates ovariectomized-induced bone loss via suppressing ROS and NFATc1 by targeting Trim38 mediated proteasomal degradation of TRAF6. AFPR reduced RANKL-mediated ROS level by down-regulating TRAF6/NOX1 signaling cascade and enhancing Nrf2-mediated ROS scavenging enzymes (HO-1, Nqo1), followed by inhibiting NF-κB and MAPK pathways, leading to the suppression of NFATc1 activity and the expression of osteoclast-related genes, such as Ctsk and Atp6v0d2. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2024
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100. MicroRNA-19b exacerbates systemic sclerosis through promoting Th9 cells.
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Lim, Yun-Ji, Park, Sang-A, Wang, Dandan, Jin, Wenwen, Ku, Wai Lim, Zhang, Dunfang, Xu, Junji, Patiño, Liliana C., Liu, Na, Chen, Weiwei, Kazmi, Rida, Zhao, Keji, Zhang, Ying E., Sun, Lingyun, and Chen, WanJun
- Abstract
Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by fibrosis of the skin and multiple vital organs, but the immunological pathogenesis of SSc remains unclear. We show here that miR-19b promotes Th9 cells that exacerbate SSc. Specifically, miR-19b and interleukin (IL)-9 increase in CD4
+ T cells in experimental SSc in mice induced with bleomycin. Inhibiting miR-19b reduces Th9 cells and ameliorates the disease. Mechanistically, transforming growth factor beta (TGF-β) plus IL-4 activates pSmad3-Ser213 and TRAF6-K63 ubiquitination by suppressing NLRC3. Activated TRAF6 sequentially promotes TGF-β-activated kinase 1 (TAK1) and nuclear factor κB (NF-κB) p65 phosphorylation, leading to the upregulation of miR-19b. Notably, miR-19b activated Il9 gene expression by directly suppressing atypical E2F family member E2f8. In patients with SSc, higher levels of IL9 and MIR-19B correlate with worse disease progression. Our findings reveal miR-19b as a key factor in Th9 cell-mediated SSc pathogenesis and should have clinical implications for patients with SSc. [Display omitted] • TGF-β and IL-4 induce miR-19b in CD4+ T cells by TRAF6-TAK1-NF-κB p65 axis • miR-19b promotes Il9 gene expression by suppressing E2f8 in Th9 cells • miR-19b -induced IL-9 is crucial for SSc pathogenesis • In patients with SSc, increased IL9 and MIR-19B exacerbate the disease progression Lim et al. find that TGF-β and IL-4 induce miR-19b via the TRAF6-TAK1-NF-κB axis, upregulating IL-9 in CD4+ T cells. Blockade of IL-9 and miR-19b in a SSc mouse model suppresses fibrogenesis. Importantly, increased IL9 and MIR-19B in patients with SSc correlates with scleroderma pathogenesis. [ABSTRACT FROM AUTHOR]- Published
- 2024
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