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286 results on '"THANY, Steeve"'

51. Caractériser les neurorécepteurs de tiques pour le développement de nouveaux acaricides plus spécifiques et durables : le projet Xenobio-Tick

Author

Plantard, Olivier, Rispe, Claude, Thany, Steeve, Charvet, Claude, Courtot, Elise, Auger, Clément, Neveu, Cédric, Biologie, Epidémiologie et analyse de risque en Santé Animale (BIOEPAR), Institut National de la Recherche Agronomique (INRA), Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut Carnot France Future Elevage, and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
tique, caractérisation fonctionnelle, transcriptomique, acaricide, [SDV.BA]Life Sciences [q-bio]/Animal biology, neurorécepteur, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

52. Caractérisation moléculaire et fonctionnelle des canaux ioniques ligand-dépendants chez Pediculus humanus

Author

Dupuy-Papin, Catherine, Debierre-Grockiego, Françoise, Toubate, Berthine, Charvet, Claude, Thany, Steeve, Neveu, Cédric, Dimier-Poisson, Isabelle, ProdInra, Migration, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO), and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
canaux ioniques ligand-dependants, [SDV.BA] Life Sciences [q-bio]/Animal biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, Pediculus humanus, pharmacologie, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

53. Sulfoximine derivative, sulfoxaflor, activates imidacloprid-sensitive nicotinic acetylcholine receptors on insect neurosecretory cells

Author

Moambi, Basile, Houchat, Jean-Noel, Cartereau, Alison, Mathe-Allainmat, Monique, Lebreton, Jacques, Graton, Jerome, Le Questel, Jean-Yves, Thany, Steeve, Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO), Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), and Récepteurs et Canaux Ioniques Membranaires (RCIM)

Subjects
[CHIM]Chemical Sciences
Abstract

256th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nanoscience, Nanotechnology and Beyond, Boston, MA, AUG 19-23, 2018

Published
2018

59. Characterization of nicotine acetylcholine receptor subunits in the cockroach Periplaneta americana mushroom bodies reveals a strong expression of β1 subunit: involvement in nicotine-induced currents

Author

Taillebois, Emiliane, Thany, Steeve Hervé, Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), and Récepteurs Canaux Ioniques Membranaires (RCIM)

Subjects
nicotinic receptors, β1 subunit, animal structures, Kenyon cells, [SDV]Life Sciences [q-bio], cockroach
Abstract

International audience; Nicotinic acetylcholine receptors are ligand-gated ion channels expressed in many insect structures, such as mushroom bodies, in which they play a central role. We have recently demonstrated using electrophysiological recordings that different native nicotinic receptors are expressed in cockroach mushroom bodies Kenyon cells. In the present study, we demonstrated that eight genes coding for cockroach nicotinic acetylcholine receptor subunits are expressed in the mushroom bodies. Quantitative real-time polymerase chain reaction (PCR) experiments demonstrated that β1 subunit was the most expressed in the mushroom bodies. Moreover, antisense oligonucleotides performed against β1 subunit revealed that inhibition of β1 expression strongly decreases nicotine-induced currents amplitudes. Moreover, co-application with 0.5 μM α-bungarotoxin completely inhibited nicotine currents whereas 10 μM d-tubocurarine had a partial effect demonstrating that β1-containing neuronal nicotinic acetylcholine receptor subtypes could be sensitive to the nicotinic acetylcholine receptor antagonist α-bungarotoxin.

Published
2016

62. Pre-treatment of Stegomyia aegypti mosquitoes with a sublethal dose of imidacloprid impairs behavioural avoidance induced by lemon oil and DEET

Author

Thany, Steeve Hervé, Tong, F., Bloomquist, Jeffrey, Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), Deployed War-Fighter Protection (DWFP) Research Program - U.S. Department of Defense through the Armed Forces Pest Management Board (AFPMB), Agriculture Research Service of the U.S. Department of Agriculture, and Récepteurs Canaux Ioniques Membranaires (RCIM)

Subjects
Insecticides, animal structures, repellency, [SDV]Life Sciences [q-bio], fungi, Imidazoles, DEET, mosquito, imidacloprid, Nitro Compounds, behaviour, Neonicotinoids, Culicidae, Aedes, Insect Repellents, parasitic diseases, Stegomyia aegypti, Avoidance Learning, Animals, Plant Oils, Female
Abstract

International audience; The present study was conducted to determine whether imidacloprid can impair the avoidance behaviour of the mosquito Stegomyia aegypti. Laboratory investigations using a T-maze apparatus showed that St. aegypti mosquitoes present long term avoidance behaviour when they are exposed to repetitive trials with lemon oil and DEET. The present study tested the effect of a sublethal dose of imidacloprid on the avoidance behaviour of St. aegypti mosquitoes over a 48 h period. Data suggest that 0.5 ng of imidacloprid/mosquito reduces the avoidance behaviour of mosquitoes exposed to lemon oil, on the first day of exposure, after the second trial; whereas imidacloprid affected DEET repellency only the first day of exposure, after the second trial. Imidacloprid was toxic against St. aegypti mosquitoes, and at sublethal doses was able to impair the repellency induced by lemon oil and DEET. The present data were consistent with the finding that St. aegypti mosquitoes exhibit long term avoidance behaviour, and treatment of mosquitoes with a sublethal dose of imidacloprid under DEET application can affect the repellency of DEET against St. aegypti.

Published
2015

64. Neonicotinoids mode of action on the pea aphid nicotinic acetylcholine receptors

Author

Taillebois, Emiliane, Tricoire-Leignel, Hélène, Thany, Steeve Hervé, Univ Angers, Okina, Récepteurs et Canaux Ioniques Membranaires (RCIM), and Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

68. Identification de cibles d’insecticides spécifiques du puceron du pois Acyrthosiphon pisum par analyse des relations structure-fonction des récepteurs nicotiniques

Author

Taillebois, Emiliane, Charvet, Claude, Araoz, Romulo, Mattei, César, Rogniaux, Helene, Tagu, Denis, Servent, Denis, Molgo, Jordi, Legros, Christian, Neveu, Cédric, Thany, Steeve H., Tricoire Leignel, Hélène, Université d'Angers (UA), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), and Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

Session 1 - Thérapies et résistances; National audience

Published
2013

69. Ca 2+ /CALMODULIN-DEPENDENT PROTEIN KINASE II IN THE COCKROACH Periplaneta americana : IDENTIFICATION OF FIVE ISOFORMS AND THEIR TISSUES DISTRIBUTION

Author

Taillebois, Emiliane, Heuland, Emilie, Bourdin, Céline, Griveau, Audrey, Quinchard, Sophie, Tricoire-Leignel, Hélène, Legros, Christian, Thany, Steeve, Stress Oxydant et Pathologies Métaboliques (SOPAM), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), Design and Application of Innovative Local Treatments in Glioblastoma (CRCINA-ÉQUIPE 17), Centre de Recherche en Cancérologie et Immunologie Nantes-Angers (CRCINA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA), Laboratoire de Planétologie et Géodynamique [UMR 6112] (LPG), Université d'Angers (UA)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), Biologie Neurovasculaire et Mitochondriale Intégrée (BNMI), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire R ecepteurs et Canaux Ioniques Membranaires (RCIM), UPRES EA 2647/USC INRA 1330/SFR 4207 QUASAV, UFR Sciences, Université d'Angers (UA), Laboratoire Récepteurs et Canaux Ioniques Membranaires (RCIM), Design and Application of Innovative Local Treatments in Glioblastoma (CRCINA - Département NOHMAD - Equipe 17), Centre de recherche de Cancérologie et d'Immunologie / Nantes - Angers (CRCINA), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Laboratoire de Planétologie et Géodynamique UMR6112 (LPG), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Université d'Angers (UA), Récepteurs Canaux Ioniques Membranaires (RCIM), Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Quinchard, Sophie, and Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)

Subjects
[SDE] Environmental Sciences, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, MESH: DNA Primers, [SDV.GEN]Life Sciences [q-bio]/Genetics, CaMKII, MESH: Alternative Splicing, [SDV]Life Sciences [q-bio], nervous system, [SDV.GEN] Life Sciences [q-bio]/Genetics, cockroach, MESH: Protein Isoforms, MESH: Gene Expression Regulation, [SDV] Life Sciences [q-bio], alternative splicing, MESH: Reverse Transcriptase Polymerase Chain Reaction, MESH: Calcium-Calmodulin-Dependent Protein Kinase Type 2, [SDE]Environmental Sciences, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Periplaneta, insect, MESH: Animals, MESH: Cloning, Molecular, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Tissue Distribution, MESH: Computational Biology
Abstract

International audience; Calcium/calmodulin-dependent protein kinase II (CaMKII) is a key kinase that transduces Ca²⁺ signals into downstream effects acting on a range of cellular processes in nervous system and muscular tissues. In insects, different CaMKII isoforms have been reported in Drosophila melanogaster, Apis florae, Bombus terrestris, and Bombus impatiens but little is known on the organization and tissue-specific expression of these isoforms with the exception of Drosophila. The present study reports the cloning of five CaMKII splice variants issued from a single gene and their tissue-specific expression in the cockroach Periplaneta americana. Each CaMKII isoform shared 82-90% identity with Drosophila CaMKII isoforms and accordingly were named PaCaMKII-A, PaCaMKII-B,PaCaMKII-C,PaCaMKII-D, and PaCaMKII-E. PaCaMKII-A and PaCaMKII-D isoforms are ubiquitously expressed in all tissues, but some such as PaCaMKII-B andPaCaMKII-C are preferentially expressed in the nerve cord and muscle. In addition, using single-cell reverse transcriptase-polymerase chain reaction (RT-PCR), we found a tissue-specific expression of PaCaMKII-E in the dorsal unpaired median neurons. Alternative splicing of PaCaMKII transcripts is likely a common mechanism in insects to control the pattern of isoform expression in the different tissues.

Published
2013

70. Calcium pathways such as cAMP modulate clothianidin action through activation of α-bungarotoxin-sensitive and -insensitive nicotinic acetylcholine receptors

Author

List, Delphine, List, Olivier, Quinchard, Sophie, Thany, Steeve, Calas-List, Delphine, Récepteurs Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), Laboratoire de Planétologie et Géodynamique UMR6112 (LPG), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Université d'Angers (UA), Laboratoire Récepteurs et Canaux Ioniques Membranaires (RCIM), Laboratoire R ecepteurs et Canaux Ioniques Membranaires (RCIM), UPRES EA 2647/USC INRA 1330/SFR 4207 QUASAV, UFR Sciences, Université d'Angers (UA), Récepteurs et Canaux Ioniques Membranaires (RCIM), Laboratoire de Planétologie et Géodynamique [UMR 6112] (LPG), Université d'Angers (UA)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), and Université de Nantes (UN)-Université de Nantes (UN)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, Insecticides, Time Factors, alpha7 Nicotinic Acetylcholine Receptor, [SDV]Life Sciences [q-bio], MESH: Neurons, Nicotinic Antagonists, cockroach, Pharmacology, Receptors, Nicotinic, Toxicology, Guanidines, Second Messenger Systems, Membrane Potentials, MESH: Dose-Response Relationship, Drug, chemistry.chemical_compound, Neonicotinoids, 0302 clinical medicine, MESH: Neonicotinoids, Mecamylamine, MESH: Insect Proteins, Cyclic AMP, Periplaneta, MESH: Animals, Nicotinic Agonists, Receptor, MESH: Cyclic AMP, Neurons, 0303 health sciences, Chemistry, General Neuroscience, Bungarotoxin, 3. Good health, MESH: Guanidines, Nicotinic agonist, DUM neurons, [SDE]Environmental Sciences, MESH: Receptors, Nicotinic, Insect Proteins, medicine.drug, Agonist, nicotinic receptors, medicine.drug_class, MESH: Thiazoles, clothianidin, MESH: Calcium Signaling, Cell Line, MESH: alpha7 Nicotinic Acetylcholine Receptor, 03 medical and health sciences, cAMP, MESH: Bungarotoxins, MESH: Nicotinic Agonists, medicine, Animals, MESH: Membrane Potentials, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Calcium Signaling, 030304 developmental biology, Acetylcholine receptor, [SDV.GEN]Life Sciences [q-bio]/Genetics, calcium, Dose-Response Relationship, Drug, MESH: Time Factors, Neonicotinoid, neonicotinoid, Clothianidin, MESH: Nicotinic Antagonists, Bungarotoxins, MESH: Male, MESH: Second Messenger Systems, MESH: Cell Line, MESH: Insecticides, Thiazoles, Biophysics, MESH: Periplaneta, insect, 030217 neurology & neurosurgery
Abstract

International audience; Clothianidin is a neonicotinoid insecticide developed in the early 2000s. We have recently demonstrated that it was a full agonist of α-bungarotoxin-sensitive and -insensitive nicotinic acetylcholine receptors expressed in the cockroach dorsal unpaired median neurons. Clothianidin was able to act as an agonist of imidacloprid-insensitive nAChR2 receptor and internal regulation of cAMP concentration modulated nAChR2 sensitivity to clothianidin. In the present study, we demonstrated that cAMP modulated the agonist action of clothianidin via α-bungarotoxin-sensitive and insensitive receptors. Clothianidin-induced current-voltage curves were dependent to clothianidin concentrations. At 10 μM clothianidin, increasing cAMP concentration induced a linear current-voltage curve. Clothianidin effects were blocked by 0.5 μM α-bungarotoxin suggesting that cAMP modulation occurred through α-bungarotoxin-sensitive receptors. At 1 mM clothianidin, cAMP effects were associated to α-bungarotoxin-insensitive receptors because clothianidin-induced currents were blocked by 5 μM mecamylamine and 20 μM d-tubocurarine. In addition, we found that application of 1mM clothianidin induced a strong increase of intracellular calcium concentration. These data reinforced the finding that calcium pathways including cAMP modulated clothianidin action on insect nicotinic acetylcholine receptors. We proposed that intracellular calcium pathways such as cAMP could be a target to modulate the mode of action of neonicotinoid insecticides.

Published
2013

71. Base moléculaire de la sensibilité aux insecticides néonicotinoïdes chez le puceron du pois

Author

Taillebois, Emiliane, Beloula, Abdelhamid, Quinchard, Sophie, Jaubert-Possamai, Stéphanie, Daguin, D, Servent, Denis, Tagu, Denis, Thany, Steeve Hervé, Tricoire-Leignel, Hélène, Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), and Univ Angers, Okina

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Published
2013

72. Neurotoxicity of pesticides: its relationship with neurodegenerative diseases

Author

Thany, Steeve H., Reynier, Pascal, Lenaers, Guy, Université d'Angers (UA), Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM), Université Montpellier 1 (UM1), and Université Montpellier 2 - Sciences et Techniques (UM2)

Subjects
SUBSTANTIA-NIGRA, PARKINSONS-DISEASE, ALPHA-SYNUCLEIN, [SDV]Life Sciences [q-bio], OXYGEN SPECIES PRODUCTION, ROTENONE EXPOSURE, OXIDATIVE STRESS, DEGENERATION, PARAQUAT, MICROGLIAL ACTIVATION, COMPLEX-I
Abstract

National audience; Neurotoxicity of pesticides: its relationship with neurodegenerative diseases Several epidemiological studies suggest that pesticides could lead to neurodegenerative diseases such as Parkinson's and Alzheimer's diseases. Among pesticides, insecticides appear more neurotoxic than others but the neurotoxic mechanisms leading to adverse health effects remain unclear. The currently used pesticides such as rotenone and paraquat could disrupt mitochondrial bioenergetic function, reactive oxygen metabolism, redox function and promote a-synuclein aggregation. In addition, recent studies demonstrate that genetic susceptibility to Parkinson's disease could monitor pesticide susceptibility, as demonstrated for polymorphisms in pesticide metabolizing enzymes that are involved in organophosphorus sensitivity.

Published
2013

73. Neonicotinoid insecticides differently modulate acetycholine-induced currents on mammalian α7 nicotinic acetylcholine receptors.

Author

Cartereau, Alison, Martin, Carine, and Thany, Steeve H

Subjects
NEONICOTINOIDS, ACETYLCHOLINE, XENOPUS, CLOTHIANIDIN, PLANT cells & tissues
Abstract

Background and Purpose: Neonicotinoid insecticides are described as poor agonists of mammalian nicotinic ACh receptors. In this paper, we show that their effects on mammalian nicotinic receptors differ between compounds.Experimental Approach: Two-electrode voltage-clamp electrophysiology was used to characterize the pharmacology of three neonicotinoid insecticides on nicotinic α7 receptors expressed in Xenopus oocytes. Single and combined application of clothianidin, acetamiprid and thiamethoxam were tested.Results: Two neonicotinoid insecticides, clothianidin and acetamiprid, were partial agonists of mammalian neuronal α7 nicotinic receptors, whereas another neonicotinoid insecticide, thiamethoxam, which is converted to clothianidin in insect and plant tissues, had no effect. Pretreatment with clothianidin and acetamiprid (10 μM) ACh significantly enhanced the subsequent currents evoked by ACh (100 μM ) whereas pretreatment with thiamethoxam (10 μM) reduced ACh-induced current amplitudes.A combination of the three neonicotinoids decreased the ACh-evoked currents.Conclusions and Implications: The present findings suggest that neonicotinoid insecticides differ markedly in their direct effects on mammalian α7 nicotinic ACh receptors and can also modulate ACh-induced currents. Furthermore, our data indicate a previously unknown modulation of mammalian α7 nicotinic receptors by a combination of clothianidin, acetamiprid and thiamethoxam.Linked Articles: This article is part of a themed section on Nicotinic Acetylcholine Receptors. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v175.11/issuetoc. [ABSTRACT FROM AUTHOR]

Published
2018
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74. Alternative splicing and subunit nature modify 3D ligand binding domain of cockroach nAChRs built by homology modelling: consequences for insecticide docking

Author

Tricoire-Leignel, Hélène, Frances, C., Thany, Steeve Hervé, Bouabid, S., Quinchard, Sophie, Lapied, Bruno, Marchand-Geneste, N., Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), and Univ Angers, Okina

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2011

75. Des insecticides plus sélectifs

Author

Legros, Christian, Tricoire-Leignel, Hélène, Thany, Steeve Hervé, Univ Angers, Okina, Récepteurs et Canaux Ioniques Membranaires (RCIM), and Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Abstract

International audience; La découverte d'un gène spécifique élargit les voies de recherche pouvant permettre de réduire les "dommages collatéraux" des insecticides.

Published
2011

76. Toxicological effect of neonicotinoid insecticides and molecular analysis of their target in pea aphid Acyrthosiphon pisum

Author

Taillebois, Emiliane, Beloula, Abdelhamid, Quinchard, Sophie, Jaubert-Possamai, Stéphanie, Thany, Steeve Hervé, Tagu, Denis, Tricoire-Leignel, Hélène, Univ Angers, Okina, Récepteurs et Canaux Ioniques Membranaires (RCIM), and Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2011

78. Positive allosteric modulation of muscarinic receptors is responsible for the synergistic effect occurring between the repellent DEET and carbamate in insect central nervous system

Author

Mohamed, Aly, Stankiewicz, Maria, Licznar, Patricia, Apaire-Marchais, Véronique, Pennetier, Cedric, Corbel, Vincent, Thany, Steeve Hervé, Lapied, Bruno, Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), and Univ Angers, Okina

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2010

79. Caractérisation moléculaire de la calmoduline et de la calmoduline kinase II chez la blatte Periplaneta americana

Author

Heuland, Emilie, Tricoire-Leignel, Hélène, Griveau, Audrey, Quinchard, Sophie, Thany, Steeve Hervé, Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), and Univ Angers, Okina

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2010

80. 3D molecular characterization of two new nicotinic acetylcholine receptor ligand binding domains in the cockroach using homology modelling: consequences for insecticide docking

Author

Tricoire-Leignel, Hélène, Frances, C., Thany, Steeve Hervé, Bouabid, S., Quinchard, Sophie, Lapied, Bruno, Marchand-Geneste, N., Univ Angers, Okina, Récepteurs et Canaux Ioniques Membranaires (RCIM), and Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Published
2010

81. 3D molecular characterization of two new nicotinic acetylcholine receptor ligand binding domains in the cockroach and analysis of insecticide binding modes using docking method

Author

Tricoire-Leignel, Hélène, Frances, C., Thany, Steeve Hervé, Bouabid, S., Quinchard, Sophie, Lapied, Bruno, Marchand-Geneste, N., Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), and Univ Angers, Okina

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2010

82. Transmembrane potential, calcium and cyclic AMP modulate selectively insect neuronal nAChR subtype sensitivity to the neurotoxic neonicotinoid insecticides

Author

Bodereau-Dubois, Béatrice, Thany, Steeve Hervé, Lapied, Bruno, Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), and Univ Angers, Okina

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2010

83. Molecular characterization of calmodulin and calmodulin kinase II in the cockroach Periplaneta americana and their involvement in the modulation of the insect nicotinic receptors

Author

Thany, Steeve Hervé, Heuland, Emilie, Griveau, Audrey, Quinchard, Sophie, Tricoire-Leignel, Hélène, Univ Angers, Okina, Récepteurs et Canaux Ioniques Membranaires (RCIM), and Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2010

84. Modulation de la sensibilité des nAChRs aux insecticides néonicotinoïdes : implications du potentiel de membrane, du calcium, des voies de signalisation et des propriétés fonctionnelles des nAChRs

Author

Bodereau-Dubois, Béatrice, Thany, Steeve Hervé, Lapied, Bruno, Univ Angers, Okina, Récepteurs et Canaux Ioniques Membranaires (RCIM), and Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2010

91. Transmembrane Potential Polarization, Calcium Influx, and Receptor Conformational State Modulate the Sensitivity of the Imidacloprid-Insensitive Neuronal Insect Nicotinic Acetylcholine Receptor to Neonicotinoid Insecticides

Author

Bodereau-Dubois, Béatrice, primary, List, Olivier, additional, Calas-List, Delphine, additional, Marques, Olivier, additional, Communal, Pierre-Yves, additional, Thany, Steeve H., additional, and Lapied, Bruno, additional

Published
2012
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99. Inhibition of PaCa MKII-E isoform in the dorsal unpaired median neurosecretory cells of cockroach reduces nicotine- and clothianidin-induced currents.

Author

List, Olivier, Calas‐List, Delphine, Taillebois, Emiliane, Juchaux, Marjorie, Heuland, Emilie, and Thany, Steeve H.

Subjects
COCKROACHES, NICOTINE, CLOTHIANIDIN, PROTEIN kinases, BUNGAROTOXIN, CHOLINERGIC receptors, NEONICOTINOIDS, PHYSIOLOGY
Abstract

Cellular responses to Ca2+ require intermediary proteins such as calcium/calmodulin-dependent protein kinase II (CaMKII), which transduces the signal into downstream effects. We recently demonstrated that the cockroach genome encodes five different Ca MKII isoforms, and only PaCa MKII-E isoform is specifically expressed in the dorsal unpaired median neurosecretory cells. In the present study, using antisense oligonucleotides, we demonstrated that PaCa MKII-E isoform inhibition reduced nicotine-induced currents through α-bungarotoxin-sensitive and -insensitive nicotinic acetylcholine receptor subtypes. Specifically, PaCa MKII-E isoform is sufficient to repress nicotinic current amplitudes as a result of its depression by antisense oligonucleotides. Similar results were found using the neonicotinoid insecticide clothianidin, which acted as a full agonist of dorsal unpaired median neuron nicotinic acetylcholine receptors. Clothianidin current amplitudes are strongly reduced under bath application of PaCa MKII-E antisense oligonucleotides but no significant results are found with α-bungarotoxin co-applied, demonstrating that Ca MKII-E isoform affects nicotine currents through α-bungarotoxin-sensitive and -insensitive receptor subtypes whereas clothianidin currents are reduced via α-bungarotoxin-insensitive receptors. In addition, we found that intracellular calcium increase induced by nicotine and clothianidin were reduced by PaCa MKII-E antisense oligonucleotides, demonstrating that intracellular calcium increase induced by nicotine and clothianidin are affected by PaCa MKII-E inhibition. [ABSTRACT FROM AUTHOR]

Published
2014
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100. Neonicotinoid Binding, Toxicity and Expression of Nicotinic Acetylcholine Receptor Subunits in the Aphid Acyrthosiphon pisum.

Author

Taillebois, Emiliane, Beloula, Abdelhamid, Quinchard, Sophie, Jaubert-Possamai, Stéphanie, Daguin, Antoine, Servent, Denis, Tagu, Denis, Thany, Steeve H., and Tricoire-Leignel, Hélène

Subjects
NEONICOTINOIDS, CARRIER proteins, NICOTINIC acetylcholine receptors, PEA aphid, THIAMETHOXAM
Abstract

Neonicotinoid insecticides act on nicotinic acetylcholine receptor and are particularly effective against sucking pests. They are widely used in crops protection to fight against aphids, which cause severe damage. In the present study we evaluated the susceptibility of the pea aphid Acyrthosiphon pisum to the commonly used neonicotinoid insecticides imidacloprid (IMI), thiamethoxam (TMX) and clothianidin (CLT). Binding studies on aphid membrane preparations revealed the existence of high and low-affinity binding sites for [3H]-IMI (Kd of 0.16±0.04 nM and 41.7±5.9 nM) and for the nicotinic antagonist [125I]-α-bungarotoxin (Kd of 0.008±0.002 nM and 1.135±0.213 nM). Competitive binding experiments demonstrated that TMX displayed a higher affinity than IMI for [125I]-α-bungarotoxin binding sites while CLT affinity was similar for both [125I]-α-bungarotoxin and [3H]-IMI binding sites. Interestingly, toxicological studies revealed that at 48 h, IMI (LC50 = 0.038 µg/ml) and TMX (LC50 = 0.034 µg/ml) were more toxic than CLT (LC50 = 0.118 µg/ml). The effect of TMX could be associated to its metabolite CLT as demonstrated by HPLC/MS analysis. In addition, we found that aphid larvae treated either with IMI, TMX or CLT showed a strong variation of nAChR subunit expression. Using semi-quantitative PCR experiments, we detected for all insecticides an increase of Apisumα10 and Apisumβ1 expressions levels, whereas Apisumβ2 expression decreased. Moreover, some other receptor subunits seemed to be differently regulated according to the insecticide used. Finally, we also demonstrated that nAChR subunit expression differed during pea aphid development. Altogether these results highlight species specificity that should be taken into account in pest management strategies. [ABSTRACT FROM AUTHOR]

Published
2014
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