97 results on '"TAN, XIAO-HUA"'
Search Results
52. Prediction on Droplet Sauter Mean Diameter in Gas-Liquid Mist Flow Based on Droplet Fractal Theory
- Author
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Liu, Jian-Yi, primary, Tan, Xiao-Hua, additional, Fan, Zhou, additional, You, Xu-Tao, additional, Li, Zhou, additional, and Zhao, Jia-Hui, additional
- Published
- 2015
- Full Text
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53. Production Decline Analysis for Two-Phase Flow in Multifractured Horizontal Well in Shale Gas Reservoirs
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Xie, Wei-Yang, primary, Li, Xiao-Ping, additional, Zhang, Lie-Hui, additional, Tan, Xiao-Hua, additional, Wang, Jun-Chao, additional, and Wang, Hai-Tao, additional
- Published
- 2015
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54. Study on Skin Factor and Productivity of Horizontal Well after Acidizing with Nonuniform Damage
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Yuan, Lin, primary, Li, Xiao-Ping, additional, Tan, Xiao-Hua, additional, and Zhang, Lie-Hui, additional
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- 2015
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55. Influence of Cylindrical Inclusion on the Elastic Field of Soft Material Loaded by Wedge Indenter
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Tan, Xiao Hua, primary, Qiu, Wei, additional, and Xiao, Xia, additional
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- 2014
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56. A New Model for the Accurate Prediction of Critical Liquid Removal Based on Energy Balance
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Tan, Xiao-Hua, additional, Li, Xiao-Ping, additional, Liu, Jian-Yi, additional, Zhao, Jia-Hui, additional, Zhang, Ji-Qiang, additional, and Tang, Chuan, additional
- Published
- 2014
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57. Analysis of permeability for transient two-phase flow in fractal porous media
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Tan, Xiao-Hua, primary, Li, Xiao-Ping, additional, Liu, Jian-Yi, additional, Zhang, Guang-Dong, additional, and Zhang, Lie-Hui, additional
- Published
- 2014
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58. Determine the Inflow Performance Relationship of Water Producing Gas Well Using Multiobjective Optimization Method
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Tan, Xiao-Hua, primary, Liu, Jian-Yi, additional, Zhao, Jia-Hui, additional, Li, Xiao-Ping, additional, Zhang, Guang-Dong, additional, Tang, Chuan, additional, and Li, Li, additional
- Published
- 2014
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59. A Pressure Transient Model for Power-Law Fluids in Porous Media Embedded with a Tree-Shaped Fractal Network
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Tan, Xiao-Hua, primary, Liu, Jian-Yi, additional, Zhao, Jia-Hui, additional, Li, Xiao-Ping, additional, Zhang, Guang-Dong, additional, and Tang, Chuan, additional
- Published
- 2014
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60. Characteristic Value Method of Well Test Analysis for Horizontal Gas Well
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Li, Xiao-Ping, primary, Yan, Ning-Ping, additional, and Tan, Xiao-Hua, additional
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- 2014
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61. Pressure Transient Analysis of Dual Fractal Reservoir
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Tan, Xiao-Hua, primary, Li, Xiao-Ping, additional, Liu, Jian-Yi, additional, Tang, Chuan, additional, and Li, Jin-man, additional
- Published
- 2013
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62. A Fractal Model for the Maximum Droplet Diameter in Gas-Liquid Mist Flow
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Tan, Xiao-Hua, primary, Liu, Jian-Yi, additional, Li, Xiao-Ping, additional, Zhang, Guang-Dong, additional, and Tang, Chuan, additional
- Published
- 2013
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63. Study on Glass Forming Ability for the Nd60-xCo15+xAl25 Alloys
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Tang, Yong Jun, primary, Xu, Hui, additional, Tan, Xiao Hua, additional, Man, Hua, additional, and Bai, Qin, additional
- Published
- 2012
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64. Molten Salt Synthesis of Anisotropy BaBi4Ti4O15 Powders in K2SO4-Na2SO4 Flux
- Author
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Gu, Yong Jun, primary, Huang, Jin Liang, additional, Li, Li Hua, additional, Zhang, Ke, additional, Wang, Xiao, additional, Li, Qian, additional, Tan, Xiao Hua, additional, and Xu, Hui, additional
- Published
- 2011
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65. A clinical study of cytokine-induced killer cells for the treatment of refractory lymphoma
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GUO, ZHI, primary, LIU, HAO, additional, HE, XUE-PENG, additional, TAN, XIAO-HUA, additional, ZHOU, YAN, additional, CHEN, XIA, additional, SHI, YU-JIE, additional, LIU, XIAO-DONG, additional, and CHEN, HUI-REN, additional
- Published
- 2011
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66. Distribution of CCR5-Δ32, CCR5m303A, CCR2-64I and SDF1-3′A in HIV-1 infected and uninfected high-risk Uighurs in Xinjiang, China
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Tan, Xiao-hua, primary, Zhang, Jing-yu, additional, Di, Chun-hong, additional, Hu, Ao-rong, additional, Yang, Lei, additional, Qu, Shen, additional, Zhao, Rong-li, additional, Yang, Pei-rong, additional, and Guo, Shu-xia, additional
- Published
- 2010
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67. Experimental Investigation of Large Deformation Fracture for Rubber Materials
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Kang, Yi Lan, primary, Xiao, X., additional, Li, Xiao Lei, additional, and Tan, Xiao Hua, additional
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- 2009
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68. Combination effect of oncolytic adenovirus therapy and herpes simplex virus thymidine kinase/ganciclovir in hepatic carcinoma animal models
- Author
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Zheng, Fei-qun, primary, Xu, Yin, additional, Yang, Ren-jie, additional, Wu, Bin, additional, Tan, Xiao-hua, additional, Qin, Yi-de, additional, and Zhang, Qun-wei, additional
- Published
- 2009
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69. Relationship between HPV6b infection and esophageal cancer in Kazakh in Xinjiang Uygur Autonomous Region
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Liao, Pei-Hua, primary, Tan, Xiao-Hua, additional, Zhang, Hai-Feng, additional, Lei, Li-Juan, additional, Zeng, Tong-Xia, additional, Chen, Bo, additional, Li, Feng, additional, Yang, Lei, additional, and Qin, Jiang-Mei, additional
- Published
- 2009
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70. Effect of Zr Addition on Glass-Forming Ability and Magnetic Properties of Fe–Nd–Al–B Alloys Prepared by Suction Casting
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Meng Tao, Bai Qin, Tan Xiao-hua, and Xu Hui
- Subjects
Zirconium ,Materials science ,Scanning electron microscope ,Alloy ,Energy-dispersive X-ray spectroscopy ,General Physics and Astronomy ,chemistry.chemical_element ,engineering.material ,Microstructure ,Casting ,law.invention ,Amorphous solid ,chemistry ,law ,engineering ,Crystallization ,Composite material - Abstract
The microstructure and magnetic behaviors of the Fe–Nd–Al–B alloys prepared by suction casting with zirconium addition are investigated. With the small amount of zirconium addition, the magnetic properties of the alloys change from hard magnetic property to soft magnetic property. The proper addition of Zr (6%) not only improves the glass forming ability, but also suppresses the crystallization. From the scanning electron microscopy of the [(Fe0.53Nd0.37Al0.10)0.96B0.04]94Zr6 alloy and the local average elemental compositions determined using energy dispersive spectroscopy analysis, the amorphous phase with a composition of Fe47Nd38Al12Zr3 in the alloy can be observed. The bulk amorphous Fe47Nd38Al12Zr3 alloy is prepared by suction casting exhibiting good glass-forming ability and soft magnetic behavior.
- Published
- 2009
71. A model for transient flow in porous media embedded with randomly distributed tree-shaped fractal networks.
- Author
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Tan, Xiao-Hua, Li, Xiao-Ping, Liu, Jian-Yi, Zhang, Lie-Hui, and Cai, Jianchao
- Subjects
- *
UNSTEADY flow , *MATHEMATICAL models , *POROUS materials , *DARCY'S law , *DIMENSIONLESS numbers , *NUMERICAL analysis - Abstract
A model for transient flow in porous media embedded with randomly distributed tree-shaped fractal networks was presented based on the fractal properties of tree-shaped capillaries and generalized Darcy's law. The dimensionless expression of flowing pressure was developed using the Laplace transform and Stehfest numerical inversion method. The bilogarithmic type curves were illustrated and the influences of different fractal factors on dimensionless flowing pressure were also discussed. The presented study indicated that the fractal characteristics for the tree-shaped fractal networks should be considered in analysis of transient flow in the heterogeneous porous media. The proposed model may be conducible to a better understanding of the mechanism for transient flow in the multi-porosity porous media. [ABSTRACT FROM AUTHOR]
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- 2015
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72. Investigation of the Bulk Nd60-xDyxFe30Al10 (x=0, 2, 5) Amorphous Alloys
- Author
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Xu, Hui, primary, Tan, Xiao Hua, additional, Qi, Nannan, additional, Wang, Qing, additional, and Dong, Yuanda, additional
- Published
- 2005
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73. Prediction of Ocular Irritancy of 26 Chemicals and 26 Cosmetic Products with Isolated Rabbit Eye (IRE) Test.
- Author
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GUO, Xiang, YANG, Xing Fen, YANG, Ying, HANS, Raabe, CAI, Jing Heng, XUE, Jin Yu, TAN, Xiao Hua, XIE, Xiao Ping, XIONG, Xi Kun, and HUANG, Jun Ming
- Abstract
Abstract: Objective: This study aims to establish and evaluate the methodology of isolated rabbit eye (IRE) test. Methods: IRE test was performed according to modifications of the in vitro toxicology (INVITTOX) Protocol No.85: Rabbit enucleated eye test by European Centre for the Validation of Alternative Methods (ECVAM), and then 26 chemicals and 26 cosmetic products were tested in both in vitro IRE and in vivo Draize tests. A statistical analysis was conducted to determine the relevance of the IRE test to the data generated in the Draize test. Results: IRE test was established successfully in our laboratory. It was shown that ranking correlation and class concordance were fairly well between the IRE test and the Draize test for 26 reference chemicals (Fisher''s Exact Test χ
2 =51.314, P<0.001; McNemar P=0.261; Gamma=0.960, P<0.001; Kappa=0.843, P<0.001) and 26 cosmetic products (Fisher''s Exact Test χ2 =15.522, P<0.001; McNemar P=0.311; Gamma=0.967, P<0.001; Kappa=0.611, P<0.001). Conclusion: IRE test was established successfully for in vitro testing of eye irritation as an alternative to Draize test. [Copyright &y& Elsevier]- Published
- 2012
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74. Study on Glass Forming Ability for the Nd60-xCo15+xAl25 Alloys
- Author
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Tang, Yong Jun, Xu, Hui, Tan, Xiao Hua, Man, Hua, and Bai, Qin
- Abstract
Bulk Nd60-xCo15+xAl25 (where x =0, 2, 5, 8, 11) sheet alloys were prepared by argon arc melting and suction casting a copper mold. Glassing forming ability (GFA) of these alloys was investigated by X-ray diffraction (XRD) and differential scanning calorimetry (DSC). Due to the dissimilarity reflected from DSC curves the thermodynamic calculation was applied. The values of Gibbs free energy (∆Gl-x (Tg)) for the amorphous alloys were gave out and some GFA criterions were adopted to make a comparison among the samples.
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- 2012
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75. Molten Salt Synthesis of Anisotropy BaBi4Ti4O15 Powders in K2SO4-Na2SO4 Flux
- Author
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Gu, Yong Jun, Huang, Jin Liang, Li, Li Hua, Zhang, Ke, Wang, Xiao, Li, Qian, Tan, Xiao Hua, and Xu, Hui
- Abstract
Anisotropic BaBi
4 Ti4 O15 powder was synthesized by a molten salt synthesis (MSS) method in K2 SO4 -Na2 SO4 flux and the effects of different process parameters such as calcining temperature, and ratio of salt to reactant (R) on the phase formation and morphology of anisotropic BaBi4 Ti4 O15 particles were also investigated. The as-synthesized powder calcined at 850-950°C exhibits a single tetragonal BaBi4 Ti4 O15 phase. The morphology of BaBi4 Ti4 O15 powder could be adjusted by changing the synthesis conditions. The average particle size (APS) of BaBi4 Ti4 O15 powder increased with R changing from 0.8 to 1.0, while it decreased with further increasing of R to 1.2. In addition, the APS increased with increasing calcining temperature and it showed an Arrhenius dependence on the temperature. The corresponding apparent activation energy for particle growth is 31.9kJ/mol for calcining temperature of 850-1000ºC.- Published
- 2011
- Full Text
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76. Investigation of the Bulk Nd60-xDyxFe30Al10 (x=0, 2, 5) Amorphous Alloys
- Author
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Xu, Hui, Tan, Xiao Hua, Qi, Nannan, Wang, Qing, and Dong, Yuanda
- Abstract
The glass-forming ability, thermal stability and magnetic properties of the Nd60-xDyxFe30Al10 (x=0, 2, 5) bulk amorphous alloys were investigated by x-ray diffraction (XRD), differential scanning calorimetry (DSC), scanning electron microscope (SEM) and the vibrating sample magnetometer (VSM). The results show that the glass forming ability of the Nd60-xDyxFe30Al10 (x=0, 2, 5) alloys decrease with increasing Dy content. The as-cast Nd60-xDyxFe30Al10 (x=0, 2, 5) alloys show hard magnetic behavior at room temperature. With increasing Dy content, the intrinsic coercivity of the alloys increase significantly while the saturation magnetization and remanence of the alloys decrease monotonously. With increasing annealed temperature, the intrinsic coercivity of the Nd55Fe30Al10Dy5 alloy decreased significantly, while the saturation magnetization and remanence decrease monotonously. The Nd55Fe30Al10Dy5 alloy shows soft magnetic behavior after annealed at 773K for 30 min.
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- 2005
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77. [Impact of assisted reproductive technology on birth weight discordance in twins].
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Li QY, Zhang H, Chen Q, Shi BJ, Tan XH, and Cui QL
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- Pregnancy, Infant, Newborn, Female, Humans, Birth Weight, Pregnancy Outcome, Infant, Premature, Infant, Low Birth Weight, Pregnancy, Multiple, Retrospective Studies, Population Surveillance, Reproductive Techniques, Assisted adverse effects, Premature Birth epidemiology, Pregnancy Complications
- Abstract
Objectives: To explore the association between assisted reproductive technology (ART) and birth weight discordance in twins (BWDT)., Methods: A retrospective analysis was conducted on twin infants born between January 2011 and December 2020 at the Third Affiliated Hospital of Guangzhou Medical University, with complete basic birth data. The impact of ART on the occurrence of BWDT was identified by the multivariate logistic regression analysis., Results: A total of 3 974 pairs of twins were included, with 1 431 conceived naturally and 2 543 through ART. Neonates in the ART group had higher birth weights than those in the naturally conceived group ( P <0.001). The incidence of BWDT was lower in the ART group compared to the naturally conceived group (16.17% vs 21.09%, P <0.001). The multivariate logistic regression analysis, adjusting for confounding factors such as maternal age, parity, pre-pregnancy body mass index, gestational diabetes, hypothyroidism, gestational age, and chorionic properties, showed no significant difference in the risk of BWDT between the ART and naturally conceived groups ( P >0.05)., Conclusions: ART is not associated with the risk of BWDT.
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- 2023
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78. Relationship of vasculogenic mimicry, SphK1 expression, and Cx43 expression to metastasis and prognosis in colorectal cancer.
- Author
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Su YJ, Zhang JX, Li SM, Tan XH, and Huang JA
- Abstract
Objective: To determine the presence of vasculogenic mimicry (VM) and expression of Sphingosine kinase 1 (SphK1) and Connexin43 (Cx43) in colorectal cancer (CRC) tissues, and to identify their inter-relationships and associations with multiple pathologic parameters., Methods: Ninety-two CRC specimens and normal pericarcinoma tissues were analyzed for expression of SphK1 and Cx43 using immunohistochemistry, and for identification of VM using CD34-periodic acid-Schiff dual staining., Results: The positive rate of SphK1 expression was greater in CRC cells than pericarcinoma cells (85.87% vs. 33.70%, P < 0.05). In contrast, the positive rate of Cx43 expression was greater in pericarcinoma cells than in CRC cells (58.70% vs. 92.39%, P < 0.05). Analysis of CRC tissues indicated that expression of SphK1 was associated with poor differentiation, advanced tumor stage, lymph node metastasis, and the presence of VM ( P < 0.05 for each comparison). Expression of Cx43 was associated with high differentiation and the presence of VM ( P < 0.05 for each comparison). Patient sex, age, tumor size, depth of invasion, and distant metastasis were unrelated to the expression of either protein. There was a significant correlation between the expression of SphK1 and Cx43 ( P < 0.05). Analysis of overall patient survival indicated that SphK1 positivity and the presence of VM were significantly associated with poor survival, but Cx43 positivity had no relationship with survival., Conclusion: SphK1 protein expression was significantly greater in CRC tissues than pericarcinoma tissues, suggesting this protein may be associated with the pathogenesis of CRC. In addition, the significant correlation between expression of SphK1 and Cx43 in CRC tissues suggests their interaction may impact the pathogenesis of CRC., Competing Interests: None., (IJCEP Copyright © 2018.)
- Published
- 2018
79. [Epidemiological and etiological characteristics of diarrheal disease among children under 5 years of age in Guangdong province, in 2012].
- Author
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Sun LM, Li H, Tan XH, Fang L, Deng AP, Mo YL, He JF, Ke CW, and Lin JY
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- Caliciviridae Infections epidemiology, Child, Preschool, China epidemiology, Diarrhea virology, Female, Genotype, Humans, Infant, Male, Rotavirus Infections epidemiology, Diarrhea epidemiology, Diarrhea microbiology
- Abstract
Objective: To analyze the epidemiological and etiological characteristics of diarrheal disease among children under 5 years of age in Guangdong province, in 2012., Methods: 64 hospitals in 21 cities were chosen as the diarrheal syndromic surveillance sites, of which 14 hospitals were selected to carry out etiological surveillance among children under 5 years of age, including isolation and culture of both Vibrio cholera and Shigella as well as nucleic acid detection of rotavirus and norovirus by PCR. Descriptive method was used to analyze data from syndromic and etiological surveillance programs on diarrheal, from 1932 parents of the children., Results: In 2012, the outpatient attendance rate on diarrheal among children under 5 years was 0.8%. The proportion of diarrheal in children under 5-year-olds was 63.5%, among the total number of diarrheal outpatients at the outpatient clinics under surveillance program. The morbidity of infectious diarrhea was 1454.5/10 million in children under 5 years of age. A total number of 1932 specimens were collected from children under 5 years of age, in the outpatient department. Among these specimens,Vibrio cholera appeared all negative but one was Shigella positive and proved to be Sh. sonnei. The positive rates of rotavirus and norovirus were 14.1% (273/1932)and 16.9% (326/1932). Both rotavirus and norovirus were found in 24 specimens, with a positive rate as 1.2% . 112 specimens were successfully gene sequenced for rotavirus, of which 33.9% as G1[P8] genotype, 25.9% as G9[P8], 12.5% as G2[P4] and 9.8% as G3[P8] respectively. 90 specimens were successfully gene-sequenced for norovirus, of which 76.7% as G II.4 genotype. Genetic subtypes of G II. 4/2006b, accounted for 50.0% and could be detected around the year except for June and December. New G II. 4/Sydney Strain_2012 was first detected in August and became the predominant in December. In addition, 5 specimens belonged to G I genotype with other 16 subtypes of G II., Conclusion: Results from our study proved that children under 5 years of age belonged to high-risk group for diarrheal disease in Guangdong province. Rotavirus and norovirus were both diverse in terms of genome.
- Published
- 2013
80. [Value of serum ferritin in measuring the activation and prognosis in systemic onset juvenile idiopathic arthritis].
- Author
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Zhou YF, Li CF, Wang J, Han TX, Kuang WY, Deng JH, Zhang JM, and Tan XH
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- Adolescent, Case-Control Studies, Child, Child, Preschool, Female, Humans, Male, Prognosis, Arthritis, Juvenile blood, Arthritis, Juvenile diagnosis, Ferritins blood
- Abstract
Objective: To explore the correlation of serum ferritin (SF) and systemic onset juvenile idiopathic arthritis (SOJIA) so as to determine the prognostic values of SF for SOJIA., Methods: All samples were collected from 92 juvenile idiopathic arthritis (JIA) patients at Beijing Children's Hospital between February 2005 to September 2012. Their age range was 2-15 years. There were macrophage activation syndrome (MAS, n = 25), polyarticular JIA (n = 33) and oligoarticular JIA (n = 30). And 47 healthy children and another 30 with acute infective diseases were selected as control groups respectively. Blood samples were collected and SF was measured in different disease phases.Other parameters include leucocyte, hemoglobin, platelet, C-reactive protein and erythrocyte sedimentation rate.Statistics of SF level at different groups as well as at different disease phases were performed., Results: The SF level of active phase SOJIA patients was significantly higher (P < 0.01) than that in Other groups. And its level in the active phase of SOJIA was significantly higher than that in patients during the recovery phase. The SF level in patients with MAS was significantly higher than that in those without MAS., Conclusion: Correlated with the course of SOJIA, the level of SF may judge the disease activity and predict the outcomes of SOJIA.
- Published
- 2013
81. [Effects of feeding patterns after hospital discharge on increase rates of growth indices in preterm and low-birth-weight infants within 3 months after birth].
- Author
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Wu F, Cui QL, Tan XH, Zhang H, Gao PM, Huang RZ, Yang JL, Ruan WF, Huang ZJ, Qu LH, Qiu XS, Zhan JY, and Gao SH
- Subjects
- Body Height, Body Weight, Breast Feeding, Female, Humans, Infant, Infant, Newborn, Male, Feeding Behavior, Infant Formula, Infant, Low Birth Weight growth & development, Infant, Premature growth & development
- Abstract
Objective: To study the effects of post-discharge formula (PDF) for preterm infants, breast milk (BM) and term infant formula (TF) on increase rates of body weight, length and head circumference in preterm and low-birth-weight infants (PLBWIs) from discharge to 3 months after birth, and to provide a reference for the choice of feeding pattern for PLBWIs., Methods: A total of 407 PLBWIs discharged from the newborn departments of ten hospitals in Guangzhou City and Foshan City in Guangdong Province, China were chosen for this study. According to feeding pattern, they were assigned to three groups: PDF-fed (n=258), BM-fed (n=58) and TF-fed (n=91). Their body weight, length and head circumference were measured at 3 months after birth, and the increase rates of growth indices relative to baseline values (at birth) were calculated and compared., Results: At 3 months after birth, the PDF-fed group had significantly greater body weight, length and head circumference than the BM-fed and TF-fed groups (P<0.05). The increase rates of body weight and length were significantly higher in the PDF-fed group than in the BM-fed and TF-fed groups (P<0.05)., Conclusions: Compared with those fed with BM and TF after discharge, the PDF-fed PLBWIs have higher increase rates of body weight and length and show greater body weight and length at 3 months after birth. However, further study is needed to investigate the long-term effects.
- Published
- 2013
82. [Directional differentiation of human adipose-derived stem cells into endothelial cells].
- Author
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Yang XF, He X, Zhang LH, He J, Liu XJ, Yang L, Tan XH, and Li YL
- Subjects
- Cells, Cultured, Humans, Tissue Engineering, Adipocytes cytology, Cell Differentiation, Endothelial Cells cytology, Stem Cells cytology
- Abstract
Objective: To investigate the possibility of directional differentiation of human adipose stem cells (hADSCs) into endothelial cells (EC), so as to provide seed cells for tissue engineered vessels., Methods: hADSCs were isolated from human adipose tissue by collagenase digestion, cultured and amplified by adherence to flasks. Then hADSCs were directionally induced to differentiate into EC by a combination of fibronectin (FN), endothelial cells support liquid (EGM2-MV) containing various growth factors and high concentration of VEGF165 (50 ng/ml). Then, the cells morphology, phenotype and function were identified., Results: Highly homologous hADSCs were obtained, and then hADSCs were directionally differentiated into EC. CD31 and CD34, the specific markers for EC, and vascular endothelial growth factor receptor (KDR) were positive by immunohistochemical staining and RT-PCR. In addition, unique Weibel-Palade bodies in EC were observed under transmission electron microscope. Functionally, hADSCs could swallow Dil-Ac-LDL and form tube-like structures in matrigel after endothelial differentiation., Conclusions: hADSCs can be successfully induced to differentiate into endothelial cells in vitro.
- Published
- 2011
83. [Influence of joint exposure to diazinon, propoxur and bisphenol A on phagocytosis of RAW264.7 cell].
- Author
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Tan XH, Huang Q, Yang XF, Li Z, Li N, Huang JM, Guo X, and Hao WD
- Subjects
- Animals, Benzhydryl Compounds, Cell Line, Drug Synergism, Environmental Exposure, Macrophages cytology, Mice, Diazinon toxicity, Macrophages drug effects, Phagocytosis drug effects, Phenols toxicity, Propoxur toxicity
- Abstract
Objective: To explore the toxicity of joint exposure to diazinon, propoxur and bisphenol A on phagocytosis., Methods: Flow cytometer was employed to detect the influence of diazinon and bisphenol A, propoxur and bisphenol A in mixture (mixed according to ratio of IC(50)) on mouse macrophage RAW264.7 cells' function to phagocyte fluorescent microspheres, adopting the percentage of phagocytic cells (PP) and the phagocytic index (PI) as measurement indicators. The final concentrations of mixture of diazinon and bisphenol A were (0.4 + 0.1), (3.6 + 0.7), (36.2 + 7.2), (43.4 + 8.7), (52.1 + 10.4), (62.5 + 12.5), (75.0 + 15.0) µg/ml; while those of mixture of propoxur and bisphenol A were (0.2 + 2.0 × 10(-2)), (2.4 + 0.2), (23.7 + 2.0), (35.6 + 3.0), (53.3 + 4.4), (80.0 + 6.7), (120.0 + 10.0) µg/ml. Then based on the dose-response relationship, a 2 × 2 factorial design was then carried out among different doses of mixture with statistical significance to statistically evaluate the interaction between diazinon and bisphenol A, propoxur and bisphenol A., Results: After the joint exposure, compared to the control group (PP = (23.6 ± 2.2)%; PI = 0.36 ± 0.03), any dose of the mixture of diazinon and bisphenol A ((52.1 + 10.4), (62.5 + 12.5), (75.0 + 15.0) µg/ml) could significantly increase the levels of PP ((29.0 ± 1.4)%, t = 3.89, P < 0.05; (30.2 ± 2.3)%, t = 4.74, P < 0.05; (35.0 ± 3.4)%, t = 8.21, P < 0.05) and PI (0.43 ± 0.03, t = 3.86, P < 0.05; 0.41 ± 0.02, t = 2.95, P < 0.05; 0.46 ± 0.03, t = 5.34, P < 0.05); while that of propoxur and bisphenol A ((35.6 + 3.0), (53.3 + 4.4), (80.0 + 6.7), (120.0 + 10.0) µg/ml) reduced the levels of PP ((20.6 ± 1.1)%, t = -3.00, P < 0.05; (20.2 ± 1.0)%, t = -3.42, P < 0.05; (19.4 ± 1.3)%, t = -4.23, P < 0.05; (18.8 ± 2.1)%, t = -4.81, P < 0.05) and PI (0.31 ± 0.01, t = -4.75, P < 0.05; 0.31 ± 0.01, t = -4.58, P < 0.05; 0.30 ± 0.01, t = -4.92, P < 0.05; 0.27 ± 0.02, t = -7.80, P < 0.05) on the contrary. The 2 × 2 factorial design was carried out between the mixture of diazinon (60.0 µg/ml; PP = (28.5 ± 3.4)%; PI = 0.49 ± 0.07) and bisphenol A (12.0 µg/ml; PP = (35.7 ± 2.7)%; PI = 0.67 ± 0.07), and the mixture of propoxur (48.0 µg/ml ; PP = (28.1 ± 2.2)%; PI = 0.48 ± 0.04) and bisphenol A (4.0 µg/ml; PP = (34.4 ± 2.7)%; PI = 0.59 ± 0.07). The mixture of diazinon and bisphenol A (PP = (30.4 ± 1.4)%, F(interaction) = 6.22, P < 0.05; PI = 0.53 ± 0.03, F(interaction) = 7.35, P < 0.05) and the mixture of propoxur and bisphenol A (PP = (27.5 ± 4.1)%, F(interaction) = 4.56, P < 0.05; PI = 0.46 ± 0.08, F(interaction) = 11.13, P < 0.05) both showed a significant antagonistic interaction on phagocytosis of RAW264.7 cell., Conclusion: It is suggested that the interactions between diazinon & bisphenol A and propoxur & bisphenol A both played the antagonistic role on phagocytic function of macrophages in vitro.
- Published
- 2011
84. Distribution of CCR5-Delta32, CCR5m303A, CCR2-64I and SDF1-3'A in HIV-1 infected and uninfected high-risk Uighurs in Xinjiang, China.
- Author
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Tan XH, Zhang JY, Di CH, Hu AR, Yang L, Qu S, Zhao RL, Yang PR, and Guo SX
- Subjects
- Adolescent, Adult, Chi-Square Distribution, China epidemiology, Female, Gene Frequency, Genetic Predisposition to Disease, HIV Infections epidemiology, Humans, Male, Middle Aged, Polymerase Chain Reaction, Polymorphism, Genetic, Prevalence, Chemokine CXCL12 genetics, HIV Infections genetics, Receptors, CCR2 genetics, Receptors, CCR5 genetics
- Abstract
Background: Genetic variants of the genes encoding HIV-1 co-receptors and their ligands, CCR5-Delta32, CCR5m303A, CCR2-64I and SDF1-3'A, are implicated in the susceptibility to HIV-1 infection, and the prevalence of these mutations varies by ethnicity. However, little is known about their distribution in Uighurs., Objectives: This study aimed at characterizing the frequency of these HIV-related gene variants in a high-risk Uighur population., Study Designs: A total of 251 HIV-1 seropositive and 238 seronegative high-risk Uighurs were recruited and their genotypes of CCR5-Delta32, CCR5m303A, CCR2-64I and SDF1-3'A were analyzed by PCR and PCR-ligase detection reaction (PCR-LDR)., Results: The allelic frequency of CCR5-Delta32, CCR5m303A, CCR2-64I and SDF1-3'A was 4.40%, 2.66%, 25.66% and 57.36%, respectively, in this population. Apparently, the Uighur population has low frequency of CCR5-Delta32 and CCR5m303A, but high frequency of CCR2-64I and SDF1-3'A. While there was no significant difference in the frequency of CCR5-Delta32, CCR2-64I and SDF1-3' A between HIV-1 seropositive and seronegative groups the frequency of CCR5m303A in HIV-1 seropositive group was significantly higher than that in seronegative group (P=0.006, OR=3.982 and 95%CI 1.514-10.476)., Conclusions: Our data suggest that the CCR5-Delta32, CCR2-64I and SDF1-3'A variants may have limited effect on protecting from HIV-1 infection in Uighurs. Rather, the CCR5m303A may be associated with the risk for HIV-1 infection in high-risk Uighurs.
- Published
- 2010
- Full Text
- View/download PDF
85. [Research on co-infections of HIV and human herpesvirus-8 among the Uygur high-risk groups in a city, Xinjiang].
- Author
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Yang PR, Guo SX, Tan XH, and Yang L
- Subjects
- Adolescent, Adult, Aged, Child, China epidemiology, Female, HIV Infections ethnology, Herpesvirus 8, Human isolation & purification, Humans, Male, Middle Aged, Young Adult, HIV Infections epidemiology, HIV Infections virology, Herpesviridae Infections epidemiology, Population Surveillance
- Abstract
Objective: To research the co-infections of HIV and human herpesvirus 8 (HHV8) in Uygur high-risk groups of HIV infection in a city Xinjiang., Methods: All 468 Uygurs at high HIV risk registered in the sentinel monitoring system in 2006 were enrolled in this study. The antibodies to HHV8 latency-associated nuclear antigens 1 (LANA1), lytic antigens open reading frame 65 (ORF65) and K8.1 were measured by enzyme linked immunosorbent assay (ELISA). Chi-square test and non-condition Logistic regression model were used for data analysis., Results: Of 468 sera samples, 67 (14.3%) were HIV and HHV8 co-infection positive.Male's HIV and HHV8 co-infection rate (22.6%, 54/239) was higher than the female's (5.7%, 13/229) (chi(2) = 27.285, P < 0.001). For those above 24 year old, HIV and HHV8 co-infection rate (15.8%, 65/412) was higher than the < 24 year old group's (3.6%, 2/56) (chi(2) = 5.987, P = 0.014). The group of Elementary school and illiterate people's HIV and HHV8 co-infection rate (20.7%, 40/193) was higher than the junior middle school and the above culture (9.8%, 27/275) (chi(2) = 10.999, P = 0.001). For the unmarried people, the co-infecting rate of HIV and HHV8 for the married, the cohabitants, the divorced or the widowers were 16.9% (14/83), 12.2% (42/345), 27.5% (11/40) respectively. There was significantly statistical difference among three marital status (chi(2) = 7.399, P = 0.025). Injecting drug users' HIV and HHV8 co-infection rate (26.5%, 50/189) was higher than non-injecting drug users' (6.1%, 17/279) (chi(2) = 38.083, P < 0.001), and stratified by gender, OR(M-H) was 4.207 (95%CI: 1.529 - 11.578). Via non-condition logistic stepwise regression analysis, only injecting drug use entered model, compared with non-injecting drug users, injecting drug users were more dangerous for HIV and HHV8 co-infecting (OR = 5.544; 95%CI: 3.081 - 9.975)., Conclusion: The HIV and HHV8 co-infection rate was higher in the Uygurs at high HIV risk in Xinjiang. Injecting drug use is a risk factor of the HIV and HHV8 co-infection, which might be one of routes of HIV and HHV8 co-infection among this group.
- Published
- 2009
86. [Seroepidemiology of Kaposi' s sarcoma-associated herpesvirus in drug users in Xinjiang, China].
- Author
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Yang PR, Tan XH, and Guo SX
- Subjects
- Adult, China epidemiology, Female, Herpesvirus 8, Human, Humans, Male, Middle Aged, Seroepidemiologic Studies, Substance-Related Disorders epidemiology, Young Adult, Herpesviridae Infections epidemiology, Substance-Related Disorders virology
- Published
- 2009
87. [Adenovirus vector encoding human KDR elicits immunity against hepatocellular carcinomas in mice].
- Author
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Tan XH, Wu B, Liu B, Liu XL, and Wang YC
- Subjects
- Adenoviruses, Human genetics, Animals, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cell Line, Tumor, Female, Humans, Mice, Mice, Inbred C57BL, T-Lymphocytes, Cytotoxic immunology, Transfection, Carcinoma, Hepatocellular genetics, Carcinoma, Hepatocellular immunology, Vascular Endothelial Growth Factor Receptor-2 immunology
- Abstract
Objective: To investigate the effect of adenovirus vector encoding human vascular endothelial growth factor receptor-2 (hVEGFR-2 or hKDR) on breaking the immune tolerance and inducing immunity against murine hepatocellular carcinomas., Methods: Human and mouse KDR cDNA were cloned from human umbilical vein endothelial cells (HUVEC) and C57BL/6 mouse embryo cells respectively using RT-PCR, and then Ad hKDR and Ad mKDR were constructed. Seven days after immunization of the mice with Ad hKDR or Ad mKDR, an analysis of cytotoxic activity of antigen-specific cytotoxic T lymphocytes (CTL) was made by lactate dehydrogenase (LDH) release assay, in which splenocytes of the immunized mice acted as effectors and Hepa 1-6/mKDR cells as the targets. In addition, the survival of the mice immunized with Hepa 1-6 hepatoma cells was checked., Results: Seven days after immunization, the 6 h killing activities of CTL elicited by the Ad hKDR were 84.3%+/-6.7%, 71.5%+/-5.2%, and 44.6%+/-4.7% at the ratio of the effectors:targets (E:T) of 100:1, 50:1, and 25:1, respectively. Correspondingly, the CTL activities by Ad mKDR were 65.2%+/-6.1%, 46.7%+/-5.0%, and 22.6%+/-3.7%. Sixty percent of the Ad hKDR-immunized mice with 5*10(6) Hepa 1-6 hepatoma cells were still alive two months after the inoculation, whereas just 40% of the Ad mKDR-immunized mice with 2*10(6) Hepa 1-6 cells survived two months. When CD8+ or CD4+ T lymphocytes were deleted in the mice the above mentioned CTL activities and protection of the mice from tumors disappeared., Conclusion: Adenovirus vector-mediated xenogeneic KDR can effectively break the immune tolerance to hepatocellular carcinomas in an animal model and induce a strong antigen-specific T cell response, which is dependent on CD8+ and CD4+ T cells.
- Published
- 2008
88. [Synergic effect of adenoviral vector-encoding human alpha-fetoprotein and interferon-gamma on immunity against hepatocellular carcinoma in mice].
- Author
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Tan XH and Zhu Q
- Subjects
- Animals, Humans, Immunization, Mice, Mice, Inbred C57BL, T-Lymphocytes, Cytotoxic immunology, Adenoviridae genetics, Interferon-gamma genetics, Liver Neoplasms, Experimental immunology, alpha-Fetoproteins genetics
- Abstract
Background & Objective: Primary hepatocellular carcinoma (HCC) is a common malignant tumor. Up to date, no effective treatment for HCC is available. This study was to investigate the synergic effect of adenoviral vector-encoding xenogeneic alpha-fetoprotein (AFP) and interferon-gamma (IFN-gamma) on the immunity against HCC in mice., Methods: IFN-gamma gene was cloned using reverse transcription-polymerase chain reaction (RT-PCR). Replication-defective adenovirus encoding both human AFP and murine IFN-gamma was constructed. The cytotoxic activity of antigen-specific cytotoxic T lymphocytes (CTLs) was detected 7 days after the intradermal immunization of C57BL/6 mice with Ad-hAFP, Ad-IFN-gamma or Ad-hAFP/IFN-gamma using standard (51)Cr release assay. The survival of mice after the subcutaneous inoculation of 5x10(6) hepatoma Hepa 1-6 cells was checked., Results: The cytotoxic activity of CTLs elicited by Ad-hAFP/IFN-gamma was much stronger than that by Ad-hAFP or Ad-IFN-gamma alone. At an effector:target (E:T) ratio of 10:1, the cytotoxic activities of CTLs in Ad-hAFP/IFN-gamma, Ad-hAFP, and Ad-IFN-gamma groups were (43.8+/-5.5)%, (28.2+/-3.2)%, and (12.8+/-1.9)%, at a ratio of 30:1, were (79.6+/-6.4)%, (51.9+/-4.3)%, and (15.6+/-2.3)%, and at a ratio of 90:1, were (88.2+/-6.3)%, (62.5+/-4.8)%, and (26.5+/-2.4)%, respectively. The tumor formation rates were 80% in Ad-hAFP-immunized mice and 100% in Ad-IFN-gamma-immunized mice at 2 months after inoculation with Hepa1-6 cells; no tumor formed in Ad-hAFP/IFN-gamma-immunized mice, and all mice in this group survived during two-month observation., Conclusion: Ad-hAFP can efficiently induce immunity against HCC in mice, and IFN-gamma enhances such an effect.
- Published
- 2008
89. [The establishment and assessment on cosmetic products using 3T3 mouse fibroblast neutral red uptake phototoxicity assay].
- Author
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Yang Y, Xiong XK, Yang XF, He XW, Huang JM, Tan XH, and Zheng SS
- Subjects
- 3T3 Cells drug effects, Animals, Animals, Newborn, Guinea Pigs, Mice, Toxicity Tests, Cosmetics toxicity, Dermatitis, Phototoxic, Fibroblasts drug effects
- Abstract
Objective: To establish the 3T3 mouse fibroblast neutral red uptake (NRU-PT) phototoxicity test method, and evaluate the practicality of the method in detecting potential phototoxicity of the cosmetic products., Methods: Fifteen phototoxic and 9 non-phototoxic chemicals were tested in our laboratories, the phototoxic potential of the test chemicals was evaluated in a prediction model in which either the photo irritation factor (PIF) or the mean photo effect (MPE) was compared with the coherence and sensitivity of the method. 20 kinds of functional cosmetics were detected and the results were analyzed by the 3T3 NRU-PT in vitro and Guinea pig skin phototoxicity test (in vivo)., Results: Both PIF and MPE of the chemicals were highly reproduced, and the correlation between in vitro and in vivo data was almost perfect. All the non-phototoxic provided a negative result, while 14 of the 15 phototoxic tested chemicals gave clear positive results. For cosmetics, the correlation between in vitro and in vivo data was consistent., Conclusion: The 3T3 NRU PT test was established successfully, it should be used as a good alternative method for assessing the phototoxic potential of the chemicals and cosmetics in China.
- Published
- 2007
90. [Enhanced translational efficacy of internal ribosomal entry site-mediated mRNA in dendritic cells contributes to efficient induction of antitumor immunity].
- Author
-
Tan XH and Wan YH
- Subjects
- Animals, Binding Sites genetics, Cell Line, Tumor, Dendritic Cells cytology, Female, Luciferases genetics, Luciferases metabolism, Melanoma, Experimental immunology, Melanoma, Experimental pathology, Melanoma, Experimental prevention & control, Mice, Mice, Inbred C57BL, Neoplasm Metastasis, Ovalbumin genetics, Ovalbumin immunology, Ovalbumin metabolism, Plasmids genetics, Protein Biosynthesis, RNA, Messenger metabolism, Ribosomes metabolism, Spleen cytology, Spleen immunology, Spleen metabolism, T-Lymphocytes, Cytotoxic cytology, T-Lymphocytes, Cytotoxic immunology, Transcription, Genetic, Transfection, Dendritic Cells immunology, Dendritic Cells metabolism, Immunotherapy, Adoptive methods, RNA, Messenger genetics
- Abstract
Objective: To develop a more economic and applicable substitute for modification of dendritic cells (DCs) by capped mRNA in induction of anti-tumor immunity., Methods: Four DNA plasmids as templates of mRNA in vitro transcription were constructed: pmRNA luciferase (Luc), pmRNA internal ribosomal entry site (IRES)-Luc, pmRNA ovalbumin (OVA), and pmRNA IRES-OVA. The translational efficiency of uncapped-Luc, capped-Luc, or IRES-Luc mRNA in murine DCs was detected via a Luc reporter system. Nine C57BL/6 mice were divided into 3 equal groups to be injected intraperitoneally with DCs transfected with IRES-OVA or capped-OVA mRNA and untreated DCs respectively, 1 weeks later the mice were injected with splenocytes wrapped with the target peptide or control peptide labeled by CFSE, and 4 hours later the mice were killed and suspension of splenocytes was made to test the activity of cytotoxic lymphocytes (CTLs). Another 30 mice were divided into 3 equal groups to undergo immunization by DCs as mentioned above, 1 week later mice melanoma cells of the line MO5 stably expressing OVA were injected the caudal vein, and 3 weeks later the mice were killed and their lungs were taken out to observe the metastasis of tumor, using OVA as a target antigen., Results: Insertion of IRES into upstream of gene of interest in mRNA transcriptional templates didn't affect the yield of mRNA in vitro transcription. The level of Luc activity expressed by IRES-Luc mRNA in the DCs was 20 times higher than that by expressed by Uncapped Luc mRNA, and one time higher than that by Capped-Luc mRNA 8 h after transfection of DC. Expression of Luc could be detected up to 96 h after the transfection with IRES-Luc and Capped-Luc mRNA. Four hours after the injection of peptides the CTL activity of the mice immunized with DCs pulsed with Capped-OVA mRNA was (28 +/- 3)%, not significantly different from that of the mice immunized with DCs pulsed with IRES-OVA mRNA [(32 +/- 4)%, P > 0.05]. Mata static melanoma nodules could be seen in all control mice, only one mouse of the Capped-OVA mRNA group, and none of the mice of the IRES-OVA mRNA group., Conclusion: IRES-containing tumor-associated antigen (TAA) mRNA as a more economic and applicable substitute fully replaces Capped-TAA mRNA for mRNA-based DC vaccines and DCs pulsed with IRES-containing TAA mRNA induces the same effective antigen-specific cellular response as the DCs pulsed with Capped-TAA mRNA.
- Published
- 2007
91. [Scanning aneugen and clastogen by micronuclei analysis using flow cytometry].
- Author
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Yang MJ, Zhou JC, Li Z, Yang XF, Huang JM, Tan XH, Cao J, and Zeng RP
- Subjects
- Animals, Flow Cytometry methods, Male, Mice, Mutagens toxicity, Reticulocytes ultrastructure, Colchicine toxicity, Cyclophosphamide toxicity, Micronuclei, Chromosome-Defective, Reticulocytes drug effects
- Abstract
Objective: To explore a flow cytometry (FCM)-based method for discriminating aneugen- or clastogen-induced micronuclei., Methods: Cells were stained with anti-CD71-FITC and PI, and the PI fluorescent signal intensity of micronucleated reticulocyte (MN-RET) in the peripheral blood of NIH mouse treated with COL or CP was detected by flow cytometry., Results: The ratio of the median of the intensity of MN-RET fluorescent signals to that of nucleated cell was low in the cyclophosphamide treated mouse, while the median was high in the colchicine treated mouse., Conclusion: The flow cytometry-based micronucleus assay can be used to discriminate primarily smaller MN induced by the clastogen exposure from the larger MN induced by an aneugen.
- Published
- 2006
92. [Ad hTRP2 - mediated immunity against melanoma is enhanced by dendritic cells pulsed with peptide].
- Author
-
Tan XH, Liu C, and Wan YH
- Subjects
- Adenoviridae genetics, Animals, CD8-Positive T-Lymphocytes immunology, Cancer Vaccines immunology, Cell Line, Tumor, Cells, Cultured, Cytotoxicity Tests, Immunologic, Dendritic Cells cytology, Female, Genetic Vectors genetics, Humans, Immunization, Secondary methods, Melanoma, Experimental immunology, Melanoma, Experimental pathology, Mice, Mice, Inbred C57BL, Survival Analysis, Dendritic Cells immunology, Intramolecular Oxidoreductases immunology, Melanoma, Experimental therapy, Membrane Proteins immunology, Peptide Fragments immunology, T-Lymphocytes, Cytotoxic immunology
- Abstract
Objective: To investigate the enhanced effect of bone marrow-derived dendritic cells (DC) pulsed with SVYDFFVWL, a MHC class I peptide located in 180-188 amino acid residues of human melanoma-associated antigen tyrosinase- related protein 2 ( hTRP2) on the immunity against melanomas elicited by adenovirus encoding hTRP2 (Ad hTRP2)., Methods: The mice were intradermally immunized with Ad hTRP2, and three weeks later with Ad hTRP2 or DC/SVYDFFVWL once more. Analysis of CTL killing activity and IFN-gamma-producing CD8 + T cells in the total CD8 + T cells of spleen were made using in vivo CTL and intracellular staining of IFN-gamma, respectively. Additionally, the survival of mice was checked after the subcutaneous inoculation with mouse melanoma B16. F10 cells., Results: The 6 h CTL killing and IFN-gamma producing CD8 +T cells in the total CD8 ' T cells of spleens were 68. 40%+/-5. 50% and 0. 67%+/-0.16% in Ad hTRP2 (priming)-Ad hTRP2 (boosting) group,28. 50%+/-6.40% and 0.22%+/-0.07% in DC/SVYDFFVWL (priming)-DC/ SVYDFFVWL (boosting) group,and 98. 90%+/-0.90% and 1.05%+/-0.21% in Ad hTRP2 (priming)-DC/ SVYDFFVWI, (boosting) group, respectively. In the tumor-bearing model, none of mice survived in DC/SVYDFFVWL (priming)-DC/SVYDFFVWL (boosting) group, and just only 40% of mice were tumor-free in Ad hTRP2 (priming) -Ad hTRP2 (boosting) group, whereas 100% of mice survived in Ad hTRP2 (priming)-DC/SVYDFFVWL (boosting) group., Conclusion: Boosting with DC/ SVYDFFVWL can significantly enhance the immunity against melanomas elicited by priming with Ad hTRP2, indicating that first priming with Ad hTRP2 and then boosting with DC/SVYDFFVWL is a potentially effective regimen for overcoming the disadvantage that anti-tumor immune response can not be significantly increased by readministration of adenovirus.
- Published
- 2006
93. [Immunization with dendritic cells infected with human AFP adenovirus vector effectively elicits immunity against mouse hepatocellular carcinomas].
- Author
-
Tan XH, Zhu Q, Liu C, Liu XL, Shao XT, and Wei B
- Subjects
- Adenoviridae genetics, Animals, CD4-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes immunology, Carcinoma, Hepatocellular pathology, Cell Line, Tumor, Cytotoxicity, Immunologic, Female, Genetic Vectors, Humans, Liver Neoplasms pathology, Mice, Mice, Inbred C57BL, Neoplasm Transplantation, Carcinoma, Hepatocellular immunology, Dendritic Cells immunology, Immunization, Liver Neoplasms immunology, alpha-Fetoproteins immunology
- Abstract
Objective: To investigate the effects of dendritic cells (DCs) infected with adenovirus vector encoding xenogeneic alpha-fetoprotein (AFP) on breaking the immune tolerance and induction of immunity against hepatocellular carcinomas., Methods: Human and mouse alpha-fetoprotein full-length cDNA were cloned from human HepG2 and mouse Hepa 1 - 6 hepatoma cell lines, respectively, using RT-PCR, and then inserted into adenoviral shuttle vectors to construct Ad hAFP and Ad mAFP. Mice were immunized with Ad hAFP-infected DC and in vitro CTL activity against Hepa 1 - 6 cells was examined by standard (51)Cr release assay. Survival was studied of the immunized mice, with or without depletion of CD8+ or CD4+ T cells, inoculated with Hepa 1 - 6 mouse hepatoma cells., Results: The lytic activity of CTL elicited by the Ad hAFP-infected DCs were much stronger than that by Ad mAFP-infected DCs. 80% of the Ad hAFP/DCs-immunized mice of the inoculated with 5 x 10(6) Hepa 1 - 6 hepatoma cells were still alive two months after inoculation. However, the Ad mAFP/DCs-immunized mice inoculated with 1 x 10(6) Hepa 1 - 6 cells were just 20% surviving two months later. Depletion of CD8+ or CD4+ T cells abolished such an antigen-specific immunity elicited by the DCs infected with Ad hAFP., Conclusion: Adenovirus vector-mediated xenogeneic AFP-infected DCs can effectively break the immune tolerance to hepatocellular carcinomas in an animal model and induce strong antigen-specific T cell response, which are dependent on CD8+ and CD4+ T cells.
- Published
- 2006
94. [A case-control study on risk factor of Kaposi's sarcoma in Xinjiang].
- Author
-
Qin JM, Li F, Tan XH, Guo SX, Wang XB, Zhang WJ, Xie JX, Huang J, Pu XM, Rui DS, and Yang L
- Subjects
- Adult, Aged, Aged, 80 and over, Case-Control Studies, China, Female, Herpesviridae Infections complications, Humans, Logistic Models, Male, Middle Aged, Multivariate Analysis, Risk Factors, Sarcoma, Kaposi complications, Sarcoma, Kaposi metabolism, Sarcoma, Kaposi epidemiology
- Abstract
Objective: To analyse related risk factors of classic Kaposi' s sarcoma in Xinjiang., Methods: A 1:4 case-control study was used and the conditional logistic regression model was performed in this study. Cases were followed up in Xinjiang while controls were selected by the same sex, nation and age within 5 years with cases., Results: Interleukin-6,vascular endothelial growth factor, beta-MG, neopterin, human herpevirus 8, were found to be associated with Kaposi's sarcoma risk in one-way variance model while beta2 -MG and human herpevirus 8 entered the multiple conditional logistic regression model, and their ORs were 1.002(95%CI: 1.000-1.003), 81.041 (95%CI: 1.790-3669.620)., Conclusion: There was a correlate relationship between beta2 -MG and classic Kaposi's sarcoma being found that human herpevirus 8 exposure related factors seemed to have played important roles on classic Kaposi's sarcoma in Xinjiang.
- Published
- 2005
95. [Xenogeneic melanoma-related antigen elicits anti-tumor immune response, companied by autoimmune injury].
- Author
-
Tan XH and Wan YH
- Subjects
- Adenoviridae genetics, Animals, Antigens, Heterophile immunology, Antigens, Neoplasm genetics, Female, Genetic Vectors genetics, Melanoma, Experimental pathology, Mice, Mice, Inbred C57BL, Neoplasm Transplantation, Random Allocation, T-Lymphocytes, Cytotoxic metabolism, Antigens, Neoplasm immunology, Autoimmunity, Immunization, Melanoma, Experimental immunology, TRPC Cation Channels immunology
- Abstract
Objective: To investigate the relationship between anti-tumor immune response and autoimmunity elicited by adenovirus-mediated xenogeneic melanoma-related antigen tyrosinase-related protein 2 (TRP2)., Methods: Mice divided into three groups were intradermally immunized by dl 70-3 (control group), Ad hTRP2, and Ad hTRP2 respectively. Analysis of the killing activity and production of interferon (IFN) gamma of tumor-specific cytotoxia T lymphocytes (CTL) from the C57BL/6 mice (3 mice per group) immunized with adenoviral vector encoding human or murine TRP2 (Ad hTRP2 or Ad mTRP2) were made using in vivo CTL and intracellular staining of IFN-gamma respectively. Additionally, the survival of mice (10 mice per group) immunized with Ad hTRP2 or Ad mTRP2 was checked after the subcutaneous inoculation with mouse melanoma B16.F10 cells., Results: The CTL lysis activity elicited by Ad hTRP2 was related to the duration of immunization, which increased up to peak one week after the immunization and subsequently decreased. Moreover, the Ad hTRP2-elicited CTL lysis was dose-dependent. The 6 h CTL killing and spleen IFN-gamma-producing CD8(+) T cells in total CD8(+) T cells by one week's immunization with Ad hTRP2 were 94% +/- 5% and 0.87% +/- 0.12%, respectively, whereas the 6 h CTL killing and spleen IFN-gamma-producing CD8(+) T cells by Ad mTRP2 were 22% +/- 8% and 0.15% +/- 0.05% respectively. On the other hand, 80%of the mice inoculated with 1 x 10(6) B16.F10 cells a week after the immunization with Ad hTRP2 were tumor-free for three months, but companied by formation of vitiligo in the Ad injection sites. However, only 20% of the Ad mTRP2-immunized mice inoculated with 1 x 10(5) B16.F10 cells survived, and no vitiligo was formatted., Conclusion: Adenovirus-mediated xenogeneic melanoma-related antigen TRP2 effectively breaks immune tolerance, thus inducing strong antigen-specific cytotoxic T cell response, simultaneously companied by obvious autoimmune injury.
- Published
- 2005
96. [Cloning of human telomerase reverse transcriptase promoter for targeted cancer therapy].
- Author
-
Li F, Tan XH, Peng XJ, Cai HB, and Hu DR
- Subjects
- Cloning, Molecular, Ganciclovir pharmacology, Gene Targeting, Genetic Therapy methods, Humans, Lung Neoplasms pathology, Simplexvirus genetics, Transfection, Tumor Cells, Cultured, Lung Neoplasms therapy, Promoter Regions, Genetic genetics, Telomerase genetics, Thymidine Kinase genetics
- Abstract
Objective: An human telomerase reverse transcriptase (hTERT) promoter was cloned to investigate the effect of simplex virus-thymidine kinase (HSV-tk) gene-ganciclovir (GCV) system under control of this promoter on lung cancer cells A549., Methods: Specific primers were designed to amplify the core hTERT promoter from HepG2 genome. A set of expression vectors encoding LacZ or tk gene under control of hTERT or hCMV promoter was constructed through standard molecular cloning methods. After the transfection with lipofectamine 2000, reverse transcriptional PCR (RT-PCR) and beta-Gal staining were performed to examine the activity of the hTERT promoter. The cytotoxic effects of GCV/tk on A549 and MRC5 cells transfected with the plasmids encoding tk gene were evaluated by MTT assay., Results: The hTERT promoter containing -208-+40 bp of the upstream sequence of human telomerase was successfully cloned. beta-Gal staining and RT-PCR were used to detect the expression of Lac Z and the transcription of tk gene under control of the hTERT promoter, respectively, in A549 rather than MRC5 cells. Cyototoxic effect of GCV was observed only in the A549 but not MRC5 cells after the transfection with pDC511 hTERT/tk, and the effect was dose-dependent. The effect, however, was observed in cells transfected with pDC518 hCMV/tk., Conclusion: The hTERT promoter cloned in this study can specifically control the target gene expression in telomerase-positive tumor cells but not the normal cells, suggesting that the HSV-tk/GCV system under control of the hTERT promoter is a promising targeted gene therapy for malignant tumors.
- Published
- 2005
97. [Interleukin-12 gene therapy for murine experimental hepatocellular carcinoma].
- Author
-
Liu Y, Hu DR, Xiong JH, Tan XH, Fan GR, Hu XL, Han JQ, and Wu YP
- Subjects
- Animals, COS Cells, Female, Liver Neoplasms, Experimental immunology, Mice, Mice, Inbred BALB C, T-Lymphocytes, Cytotoxic immunology, Genetic Therapy, Interleukin-12 genetics, Liver Neoplasms, Experimental therapy
- Published
- 2004
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