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Reproducible climate reconstructions of the Common Era (1 CE to present) are key to placing industrial-era warming into the context of natural climatic variability. Here we present a community-sourced database of temperature-sensitive proxy records from the PAGES2k initiative. The database gathers 692 records from 648 locations, including all continental regions and major ocean basins. The records are from trees, ice, sediment, corals, speleothems, documentary evidence, and other archives. They range in length from 50 to 2000 years, with a median of 547 years, while temporal resolution ranges from biweekly to centennial. Nearly half of the proxy time series are significantly correlated with HadCRUT4.2 surface temperature over the period 1850-2014. Global temperature composites show a remarkable degree of coherence between high- and low-resolution archives, with broadly similar patterns across archive types, terrestrial versus marine locations, and screening criteria. The database is suited to investigations of global and regional temperature variability over the Common Era, and is shared in the Linked Paleo Data (LiPD) format, including serializations in Matlab, R and Python., PAGES, a core project of Future Earth, is supported by the U.S. and Swiss National Science Foundations. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Some of this work was conducted as part of the North America 2k Working Group supported by the John Wesley Powell Center for Analysis and Synthesis, funded by the U.S. Geological Survey. B. Bauer, W. Gross, and E. Gille (NOAA National Centers for Environmental Information) are gratefully acknowledged for helping assemble the data citations and creating the NCEI versions of the PAGES 2k data records. We thank all the investigators whose commitment to data sharing enables the open science ethos embodied by this project.

The primary role of dystrophin, a 427 kDa elongated protein, is to stabilize the membrane of muscle cells against the mechanical forces deriving from muscle contraction and relaxation; its absence or mutation lead to various forms of muscular dystrophy. Dystrophin binds to actin through its N-terminus actin binding domain (ABD1), and connects to the cell membrane through its C-terminus; in between, 24 spectrin repeats (SR) with interspersed hinges provide structural flexibility and have been proposed to actively dissipate the mechanical stress. Yet, dystrophin's mechanism of function is largely unknown, preventing effective drug development. Our working hypothesis is that dystrophin's domains are allosterically coupled through specific hydrophobic interactions, and that the response to mechanical stress is propagated across domains through order-to-disorder transitions at their interface. We test this hypothesis with all-atom molecular dynamics simulations of dystrophin's SR 1 monomer, SR 17-18 dimer, and the ABD1 domain. Our results support the hypothesis by showing that (a) forced unfolding by pulling initiates at the interface between SR repeats; (b) the linker between SR 17-18 is flexible and allows for distinct conformations at the interface between domains, characterized by different hydrophobic interaction patterns; (c) mutual information analyses show that conformational changes at the dimer's interface and termini are coupled; and (d) hydrophobic interactions between the two CH domains and the connecting linker stabilize a closed conformation of ABD1 that is alternative to the open crystal structure and explains experimental fluorescence results. Our results suggest that the disease may originate from altered hydrophobic interactions, and pave the way for targeted drug development.

Human-driven changes in the global environment pose an increasingly urgent challenge for the management of ecosystems that is made all the more difficult by the uncertain future of both environmental conditions and ecological responses. Land managers need strategies to increase regional adaptive capacity, but relevant and rapid assessment approaches are lacking. To address this need, we developed a method to assess regional protected area networks across biophysically important climatic gradients often linked to biodiversity and ecosystem function. We plot the land of the southwestern United States across axes of historical climate space, and identify landscapes that may serve as strategic additions to current protected area portfolios. Considering climate space is straightforward, and it can be applied using a variety of relevant climate parameters across differing levels of land protection status. The resulting maps identify lands that are climatically distinct from existing protected areas, and may be utilized in combination with other ecological and socio-economic information essential to collaborative landscape-scale decision-making. Alongside other strategies intended to protect species of special concern, natural resources, and other ecosystem services, the methods presented herein provide another important hedging strategy intended to increase the adaptive capacity of protected area networks.

Spatial and temporal patterns of variability in spring onset are identified across western North America using a spring index (SI) model based on weather station minimum and maximum temperatures (Tmin and Tmax, respectively). Principal component analysis shows that two significant and independent patterns explain roughly half of the total variance in the timing of spring onset from 1920 to 2005. However, these patterns of spring onset do not appear to be linear responses to the primary modes of variability in the Northern Hemisphere: the Pacific–North American pattern (PNA) and the northern annular mode (NAM). Instead, over the period when reanalysis data and the spring index model overlap (1950–2005), the patterns of spring onset are local responses to the state of both the PNA and NAM, which together modulate the onset date of spring by 10–20 days on interannual time scales. They do so by controlling the number and intensity of warm days. There is also a regionwide trend in spring advancement of about −1.5 days decade−1 from 1950 to 2005. Trends in the NAM and PNA can only explain about one-third (−0.5 day decade−1) of this trend.

Bristlecone pine trees are exceptionally long-lived, and with the incorporation of remnant material have been used to construct multi-millennial length ring–width chronologies. These chronologies can provide valuable information about past temperature and moisture variability. In this study, we outline a method to build a moisture-sensitive bristlecone chronology and assess the robustness and consistency of this sensitivity over the past 1200 yr using new reconstructions of Arkansas River flow (AD 1275–2002 and 1577–2002) and the summer Palmer Drought Sensitivity Index. The chronology, a composite built from parts of three collections in the central Rocky Mountains, is a proxy for decadal-scale moisture variability for the past 18 centuries. Since the sample size is small in some portions of the time series, the chronology should be considered preliminary; the timing and duration of drought events are likely the most robust characteristics. This chronology suggests that the region experienced increased aridity during the medieval period, as did much of western North America, but that the timing and duration of drought episodes within this period were somewhat different from those in other western locations, such as the upper Colorado River basin.

Climate warming in the mid- to high-latitudes and high-elevation mountainous regions is occurring more rapidly than anywhere else on Earth, causing extensive loss of glaciers and snowpack. However, little is known about the effects of climate change on alpine stream biota, especially invertebrates. Here, we show a strong linkage between regional climate change and the fundamental niche of a rare aquatic invertebrate—the meltwater stonefly Lednia tumana—endemic to Waterton-Glacier International Peace Park, Canada and USA. L. tumana has been petitioned for listing under the U.S. Endangered Species Act due to climate-change-induced glacier loss, yet little is known on specifically how climate impacts may threaten this rare species and many other enigmatic alpine aquatic species worldwide. During 14 years of research, we documented that L. tumana inhabits a narrow distribution, restricted to short sections (~500 m) of cold, alpine streams directly below glaciers, permanent snowfields, and springs. Our simulation models suggest that climate change threatens the potential future distribution of these sensitive habitats and the persistence of L. tumana through the loss of glaciers and snowfields. Mountaintop aquatic invertebrates are ideal early warning indicators of climate warming in mountain ecosystems. Research on alpine invertebrates is urgently needed to avoid extinctions and ecosystem change.

The northern Rocky Mountains (NRMs) are a critical headwaters region with the majority of water resources originating from mountain snowpack. Observations showing declines in western U.S. snowpack have implications for water resources and biophysical processes in high-mountain environments. This study investigates oceanic and atmospheric controls underlying changes in timing, variability, and trends documented across the entire hydroclimatic-monitoring system within critical NRM watersheds. Analyses were conducted using records from 25 snow telemetry (SNOTEL) stations, 148 1 April snow course records, stream gauge records from 14 relatively unimpaired rivers, and 37 valley meteorological stations. Over the past four decades, midelevation SNOTEL records show a tendency toward decreased snowpack with peak snow water equivalent (SWE) arriving and melting out earlier. Temperature records show significant seasonal and annual decreases in the number of frost days (days ≤0°C) and changes in spring minimum temperatures that correspond with atmospheric circulation changes and surface–albedo feedbacks in March and April. Warmer spring temperatures coupled with increases in mean and variance of spring precipitation correspond strongly to earlier snowmeltout, an increased number of snow-free days, and observed changes in streamflow timing and discharge. The majority of the variability in peak and total annual snowpack and streamflow, however, is explained by season-dependent interannual-to-interdecadal changes in atmospheric circulation associated with Pacific Ocean sea surface temperatures. Over recent decades, increased spring precipitation appears to be buffering NRM total annual streamflow from what would otherwise be greater snow-related declines in hydrologic yield. Results have important implications for ecosystems, water resources, and long-lead-forecasting capabilities.

article i nfo Hydraulic conductivity K, as the coefficient of proportionality in Darcy's Law, is critical in understanding the past Martian hydrologic cycle, climate, and landform evolution. However, K and its spatial variability on Mars are thus far poorly constrained due to lack of accessibility. Using an innovative method based on surface drainage dissection patterns, which has been successfully tested in the Oregon Cascades on Earth, we estimated K in the Mare Tyrrhenum Quadrangle on Mars. The basic assumption is that under long-term dynamic equilibrium conditions, the overall dissection pattern in a watershed as reflected in drainage density is controlled by the interplay among surface runoff, groundwater flow, topography, and aquifer properties. K is calculated following a derivative of Darcy's Law under DuPuit-Forchheimer assumptions with drainage density D, valley depth d, recharge rate R, and aquifer thickness H as inputs. The results are consistent with the published K values and reveal spatial variability.

This study introduces a new method for estimating hydraulic conductivity based on the concept of effective groundwater drainage length and DuPuit-Forchheimer assumptions. The effective groundwater drainage length is related to the surface drainage dissection patterns (as expressed in drainage density) forming over long periods of time. Application of the new method to the Oregon Cascades yielded hydraulic conductivity values similar to those documented in the literature. This method represents an effective and efficient way of estimating hydraulic conductivity for regions where the interplay among surface drainage, groundwater, and topography has established a steady-state dynamic equilibrium. It also provides a theoretically sound approach for extrapolating limited local measurements to a large region and revealing the spatial variation of hydraulic conductivity.

Potential economic impacts of future climate change on crop enterprise net returns and annual net farm income (NFI) are evaluated for small and large representative farms in Flathead Valley in Northwest Montana. Crop enterprise net returns and NFI in an historical climate period (1960-2005) and future climate period (2006-2050) are compared when agricultural production systems (APSs) are adapted to future climate change. Climate conditions in the future climate period are based on the A1B, B1, and A2 CO(2) emission scenarios from the Intergovernmental Panel on Climate Change Fourth Assessment Report. Steps in the evaluation include: (1) specifying crop enterprises and APSs (i.e., combinations of crop enterprises) in consultation with locals producers; (2) simulating crop yields for two soils, crop prices, crop enterprises costs, and NFIs for APSs; (3) determining the dominant APS in the historical and future climate periods in terms of NFI; and (4) determining whether NFI for the dominant APS in the historical climate period is superior to NFI for the dominant APS in the future climate period. Crop yields are simulated using the Environmental/Policy Integrated Climate (EPIC) model and dominance comparisons for NFI are based on the stochastic efficiency with respect to a function (SERF) criterion. Probability distributions that best fit the EPIC-simulated crop yields are used to simulate 100 values for crop yields for the two soils in the historical and future climate periods. Best-fitting probability distributions for historical inflation-adjusted crop prices and specified triangular probability distributions for crop enterprise costs are used to simulate 100 values for crop prices and crop enterprise costs. Averaged over all crop enterprises, farm sizes, and soil types, simulated net return per ha averaged over all crop enterprises decreased 24% and simulated mean NFI for APSs decreased 57% between the historical and future climate periods. Although adapting APSs to future climate change is advantageous (i.e., NFI with adaptation is superior to NFI without adaptation based on SERF), in six of the nine cases in which adaptation is advantageous, NFI with adaptation in the future climate period is inferior to NFI in the historical climate period. Therefore, adaptation of APSs to future climate change in Flathead Valley is insufficient to offset the adverse impacts on NFI of such change.

The physical science linking human-induced increases in greenhouse gasses to the warming of the global climate system is well established, but the implications of this warming for ecosystem processes and services at regional scales is still poorly understood. Thus, the objectives of this work were to: (1) describe rates of change in temperature averages and extremes for western Montana, a region containing sensitive resources and ecosystems, (2) investigate associations between Montana temperature change to hemispheric and global temperature change, (3) provide climate analysis tools for land and resource managers responsible for researching and maintaining renewable resources, habitat, and threatened/endangered species and (4) integrate our findings into a more general assessment of climate impacts on ecosystem processes and services over the past century. Over 100 years of daily and monthly temperature data collected in western Montana, USA are analyzed for long-term changes in seasonal averages and daily extremes. In particular, variability and trends in temperature above or below ecologically and socially meaningful thresholds within this region (e.g., −17.8°C (0°F), 0°C (32°F), and 32.2°C (90°F)) are assessed. The daily temperature time series reveal extremely cold days (≤ −17.8°C) terminate on average 20 days earlier and decline in number, whereas extremely hot days (≥32°C) show a three-fold increase in number and a 24-day increase in seasonal window during which they occur. Results show that regionally important thresholds have been exceeded, the most recent of which include the timing and number of the 0°C freeze/thaw temperatures during spring and fall. Finally, we close with a discussion on the implications for Montana’s ecosystems. Special attention is given to critical processes that respond non-linearly as temperatures exceed critical thresholds, and have positive feedbacks that amplify the changes.

Natural snow avalanches have periodically damaged infrastructure and disrupted railroad and highway traffic at the southwestern corner of Glacier National Park, Montana. The 94-year history of these disruptions constitutes an uncommon record of natural avalanches spanning over nine decades and presents a unique opportunity to examine how natural avalanche frequency and minimum extent have varied over time due to climatic or biophysical changes. This study compared the historic record of natural avalanches in one avalanche path with tree-ring evidence of avalanches from 109 cross sections and increment cores collected in the same path. Results from combined historic and tree-ring records yielded 27 avalanche years in the 1910–2003 chronology, with the historic record alone underestimating avalanche years by half. Mean return period was 3.2 years. Interpolated maps allowed for more spatially precise estimates of return periods throughout the runout zone than previous studies. The maps show return p...

Knowledge of climatic variability at small spatial extents (< 50 km) is needed to assess vulnerabilities of biological reserves to climate change. We used empirical and modeled weather station data to test if climate change has increased the synchrony of surface air temperatures among 50 sites within the Greater Yellowstone Area (GYA) of the interior western United States. This important biological reserve is the largest protected area in the Lower 48 states and provides critical habitat for some of the world's most iconic wildlife. We focused our analyses on temporal shifts and shape changes in the annual distributions of seasonal minimum and maximum air temperatures among valley-bottom and higher elevation sites from 1948-2012. We documented consistent patterns of warming since 1948 at all 50 sites, with the most pronounced changes occurring during the Winter and Summer when minimum and maximum temperature distributions increased. These shifts indicate more hot temperatures and less cold temperatures would be expected across the GYA. Though the shifting statistical distributions indicate warming, little change in the shape of the temperature distributions across sites since 1948 suggest the GYA has maintained a diverse portfolio of temperatures within a year. Spatial heterogeneity in temperatures is likely maintained by the GYA's physiographic complexity and its large size, which encompasses multiple climate zones that respond differently to synoptic drivers. Having a diverse portfolio of temperatures may help biological reserves spread the extinction risk posed by climate change.

Instrumental climate records suggest that summer precipitation and winter snowpack in Glacier National Park (Glacier NP), Montana, vary significantly over decadal to multidecadal time scales. Because instrumental records for the region are limited to the twentieth century, knowledge of the range of variability associated with these moisture anomalies and their impacts on ecosystems and physical processes are limited. The authors developed a reconstruction of summer (June–August) moisture variability spanning a.d. 1540–2000 from a multispecies network of tree-ring chronologies in Glacier NP. Decadal-scale drought and pluvial regimes were defined as any event lasting 10 yr or greater, and the significance of each potential regime was assessed using intervention analysis. Intervention analysis prevents single intervening years of average or opposing moisture conditions from ending what was otherwise a sustained moisture regime. The reconstruction shows numerous decadal-scale shifts between persistent drought and wet events prior to the instrumental period (before a.d. 1900). Notable wet events include a series of three long-duration, high-magnitude pluvial regimes spanning the end of the Little Ice Age (a.d. 1770–1840). Though the late-nineteenth century was marked by a series of >10 yr droughts, the single most severe dry event occurred in the early-twentieth century (a.d. 1917–41). These decadal-scale dry and wet events, in conjunction with periods of high and low snowpack, have served as a driver of ecosystem processes such as forest fires and glacial dynamics in the Glacier NP region. Using a suite of paleoproxy reconstructions and information from previous studies examining the relationship between climate variability and natural processes, the authors explore how such persistent moisture anomalies affect the delivery of vital goods and services provided by Glacier NP and surrounding areas. These analyses show that regional water resources and tourism are particularly vulnerable to persistent moisture anomalies in the Glacier NP area. Many of these same decadal-scale wet and dry events were also seen among a wider network of hydroclimatic reconstructions along a north–south transect of the Rocky Mountains. Such natural climate variability can, in turn, have enormous impacts on the sustainable provision of natural resources over wide areas. Overall, these results highlight the susceptibility of goods and services provided by protected areas like Glacier NP to natural climate variability, and show that this susceptibility will likely be compounded by the effects of future human-induced climate change.

Interpretation of ground water level changes in a developed aquifer usually relies on reference to some benchmark such as “predevelopment” ground water levels, changes from fall to fall and/or spring to spring, or to determination of maximum stress during the pumping season. The assumption is that ground water levels measured in the monitoring well accurately reflect the state of the ground water resource in terms of quantity in storage and the effects of local pumping. This assumption is questionable based on the patterns shown in continuous hydrographs of water levels in monitoring wells in Nebraska, and wells installed to determine vertical gradients. These hydrographs show clear evidence for vertical ground water gradients and recharge from overlying parts of the aquifer system to deeper zones in which production wells are screened. The classical concept of semi-perched ground water, as described by Meinzer, is demonstrated by these hydrographs. The presence of semi-perched ground water (Meinzer definition, there is no intervening unsaturated zone) invalidates the use of measured ground water levels in regional observation programs for detailed numerical management of the resource. Failure to recognize the Meinzer effect has led to faulty management. The best use of data from the observation well network would be for detection of trends and education unless it is clearly understood what is being measured.

Ground-water level decline patterns in parts of Nebraska conform to the circular island concept of Bredehoeft et al. (1982), which indicates how water is derived by wells developed in a circular island. If elongated, the center of the island corresponds to a regional ground-water divide while the shoreline corresponds to a regional river. In both versions, ground-water table elevation is a function of recharge and transmissivity. A dynamic equilibrium exists such that the gradient of the water table will convey all recharge to discharge areas. Withdrawals of ground water result initially in mining, with a new equilibrium attained when pumping equals capture. During early development, capture is an important source of water in discharge areas, while mining is more significant in recharge areas. The pattern observed in many areas shows the greatest ground-water level decline in the vicinity of ground-water divides and the steepest gradient near regional rivers. A similar pattern has been observed adjacent to the Arkansas River in south-central Kansas. Similar decline patterns can be modeled for a hypothetical ground-water basin. This is of major importance to water-resource managers because it dictates that management programs be applied to the entire hydrologic system.

The Kansas-Nebraska Big Blue River compact requires that the state of Nebraska insure a minimum flow of the Big Blue River across the state line. There are two options that the state of Nebraska may use to ensure minimum flows. The obvious option is to limit surface-water irrigators along the river. However, under the terms of the compact, a second option may be to regulate irrigation wells that are within one mile of the river and were installed after November 1, 1968. The objective of this study is to quantify the effects of 17 irrigation wells that may be regulated on baseflow of the Big Blue River. A finite-element model is used to study the hydrogeologic system between DeWitt and Beatrice, Nebraska. The 17 wells that may be regulated are located between these towns and are developed in sediments deposited in a cross-cutting paleovalley anchor alluvium associated with the Big Blue River. While there wore considerable existing data, additional data were gathered by drilling an additional nine test holes, conducting several aquifer tests, stream-stage measurements, and baseflow calculation through extensive stream-discharge measurements, establishment of a ground water-level monitoring network, determining the amount of water pumped for irrigation and municipal use in the area, and a short-term precipitation network. The model was calibrated using observed baseflow and ground water level data. The model clearly shows that regulating the 17 wells to maintain baseflow would have a minimal effect on the overall water budget. This is reasonable, especially considering that there are over 250 irrigation wells in the project area. The 17 wells considered pumped only 6 percent of the total pumpage within the modeled area during the irrigation season of 1984. The computer model provides the documentation needed to demonstrate this fact. Although much of the resources spent and a significant amount of hydrogeologic data are being collected over a period of three years on a relatively small area, the simulation model could be improved through further field testing of the aquifer and stream-bed sediment characteristics and quantification of ground water recharge, discharge, and evapotranspiration rates.

Over the 20th century, surface water temperatures have increased in many lake ecosystems around the world, but long-term trends in the vertical thermal structure of lakes remain unclear, despite the strong control that thermal stratification exerts on the biological response of lakes to climate change. Here we used both neo- and paleoecological approaches to develop a fossil-based inference model for lake mixing depths and thereby refine understanding of lake thermal structure change. We focused on three common planktonic diatom taxa, the distributions of which previous research suggests might be affected by mixing depth. Comparative lake surveys and growth rate experiments revealed that these species respond to lake thermal structure when nitrogen is sufficient, with species optima ranging from shallower to deeper mixing depths. The diatom-based mixing depth model was applied to sedimentary diatom profiles extending back to 1750 AD in two lakes with moderate nitrate concentrations but differing climate settings. Thermal reconstructions were consistent with expected changes, with shallower mixing depths inferred for an alpine lake where treeline has advanced, and deeper mixing depths inferred for a boreal lake where wind strength has increased. The inference model developed here provides a new tool to expand and refine understanding of climate-induced changes in lake ecosystems.

[1] The High Plains Aquifer (HPA), underlying parts of 8 states from South Dakota to Texas, is one of the largest fresh water aquifers in the world and accounts for 30% of the groundwater used for irrigation in the US. Determining the distribution of HPA's hydraulic conductivity (K) is critical for water management and addressing water quality issues. K is traditionally estimated from well pumping data coupled with computer modeling and is known to be highly variable, spanning several orders of magnitude for the same type of rock. Here we show that applying our innovative method of determining effective horizontal K to HPA (based on surface drainage patterns and a dynamic equilibrium assumption) produced results generally consistent with those from traditional methods but reveals much more detailed spatial variation. With the exception of a few places such as the Sand Hills area, our results also show for the first time (to the best of our knowledge) a distinct relationship between surface stream drainage density and subsurface aquifer K in a major aquifer system on a regional scale. Because aquifer particle size strongly controls K, our results can be used to study patterns of past sediment movement and deposition.

Lincoln's municipal wellfield consists of 44 wells located adjacent to the Platte River near Ashland, Nebraska. The herbicide atrazine was monitored in the river and two transects of monitoring, wells. The amount of atrazine transported down the Platte River in 1989, 1990, and 1991 was shown to increase each year. Induced recharge from the Platte River results in movement of atrazine from the river into the aquifer. A 21-day lag time was determined for the movement of atrazine from the river to a transect of monitoring wells 10 feet West of the bank. The role that colloids play on the transport of atrazine was determined to be insignificant. A small percentage of atrazine found in the river was determined to come from rain water. The infiltration of agrichemical-contaminated river water was shown to significantly reduce the quality of raw water and finished water being produced by the adjacent aquifer.

Lincolns municipal wellfield consists of 44 wells developed in an alluvial aquifer adjacent to the Platte River near Ashland, Nebraska Induced recharge from the river is the primary source of water for the wellfield. Wafer samples were collected on a periodic basis from the Platte River arid two transects of monitoring wells. These samples were analyzed for the herbicide atrazine, which was used as a tracer of induced recharge in this stream-aquifer system. Atrazine concentrations in the river and aquifer were much less than 1.0 ppb during late fall and winter, but increased to as high as 18.9 ppb during spring and summer, associated with runoff from upgradient agricultural lands. There was approximately a 21-day lag time from the first detection of increasing atrazine concentration in the river to the first detection in monitoring wells immediately adjacent to the river. This lag time was relatively constant throughout the year and from one year to the next, even with major fluctuations of river stage and wellfield production. This consistency of lag time indicated that the travel times from the river to the first set of monitoring wells immediately adjacent to the river were fairly constant. Paths of preferential flow were identified in the aquifer at a depth of 25 to 35 feet below land surface. This aquifer zone appeared to play a significant role in movement of water from beneath the river into the wellfield. Aquifer dispersivity was calculated using a method described by Hoehn and Santschi (1987). Macrodispersivity (AL) was shown to increase linearly over the scale of the wellfield. Calculated values of AL were within limits of other reported values for this type of aquifer material and agreed well with values reported by Hoehn and Santschi (1987); These findings will be extremely beneficial for planning and management of the municipal wellfield.

The city of Lincoln draws water from a well field along the banks of the Platte River in east-central Nebraska. We have been able to follow the infusion of atrazine into this well field under induced recharge. Samples of water from the river, several monitoring wells and a production well were analyzed by GC/MS and solid phase extraction and found to change in concentration over the range 0.1 and 5.0 ppb of atrazine through the spring, summer, and fall 1989. Increases in the concentration of atrazine relating to precipitation events in the Platte River Basin were observable in the well water samples and ultimately in Lincoln municipal tap water. Atrazine from the river was seen to move via induced recharge and into well field ground water and away from the river at an observable rate. The concentration of atrazine in the river is dependent upon time of year and precipitation in the river basin.

Hybridization in flowering plants is determined in part by the rate at which animal pollinators move between species and by the effectiveness of such movements in transferring pollen. Pollinator behavior can also influence hybrid fitness by determining receipt and export of pollen. We incorporated information on pollinator effectiveness and visitation behavior into a simulation model that predicts pollen transfer between Ipomopsis aggregata, Ipomopsis tenuituba, and hybrids. These predictions were compared with estimates of pollen transfer derived from movement of fluorescent dyes in experimental plant arrays. Interspecific pollen transfer was relatively uncommon in these arrays, whereas transfer between hybrids and the parental species was at least as common as conspecific transfer. Backcrossing was asymmetrical; I. aggregata flowers frequently received mixed loads of hybrid and conspecific pollen. The simulation suggests that these patterns of pollen transfer are largely explained by the visitation sequences of hummingbird and insect pollinators, with little contribution from mechanical isolation. Pollen receipt by hybrids exceeded that of both parental species in a year when pollinators preferred to visit F(1) and F(2) hybrids and was intermediate in another year when they preferred to visit I. aggregata. This suggests that natural variation in pollination may produce spatiotemporal variation in hybridization and hybrid fitness.

The constraint diagram language was designed to be used in conjunction with the Unified Modelling Language (UML), primarily for placing formal constraints on software models. In particular, constraint diagrams play a similar role to the textual object constraint language in that they can be used for specifying system invariants and operation contracts in the context of a UML model. Constraint diagrams can also be used independently of the UML. In this paper, we illustrate a range of counter-intuitive features of constraint diagrams and highlight some (potential) expressiveness limitations. We propose a generalized version of the constraint diagram language that overcomes the illustrated counter-intuitive features and limitations.

Our industry is uniquely characterised by fierce competition to gain access to opportunities such as exploration acreage or participation rights in redevelopment opportunities, and once access is secured cooperation among competitors turned partners is common. In practice, the motivation for the formation of these partnerships is to reduce financial exposure and not to create value. As operators typically assume a dominant role, they drive investment decisions with little, if any, value-creating input from partners. The ultimate development plans therefore may not be attractive to the nonoperating partners, but the alternative of nonparticipation is even worse. These behaviors create a lose-lose situation. For operators, "partner-drag" extends cycle time and increases costs. For nonoperators, value creation opportunities are lost and their control over the future of their investments is less than they are entitled to, based on their equity. This paper explores some critical questions: Do partnerships create or destroy value? What role should a non-operating partner play in a venture, and what competency does it require to be able to make positive contributions? Should partners focus on errors of omission (i.e., missing high-value opportunities, doing the wrong project) or on errors of commission (i.e., not getting full value from execution, doing the project wrong)? The authors, drawing on Petoro case studies, will share lessons learned and best practices developed from implementing a business model based entirely on creating value as a nonoperating partner. Novel work processes are presented, based on these learnings, that improve the relationship between operators and nonoperating partners from lose-lose to win-win.

[1] Little Ice Age (14th–19th centuries A.D.) glacial maxima and 20th century retreat have been well documented in Glacier National Park, Montana, USA. However, the influence of regional and Pacific Basin driven climate variability on these events is poorly understood. We use tree-ring reconstructions of North Pacific surface temperature anomalies and summer drought as proxies for winter glacial accumulation and summer ablation, respectively, over the past three centuries. These records show that the 1850's glacial maximum was likely produced by ∼70 yrs of cool/wet summers coupled with high snowpack. Post 1850, glacial retreat coincides with an extended period (>50 yr) of summer drought and low snowpack culminating in the exceptional events of 1917 to 1941 when retreat rates for some glaciers exceeded 100 m/yr. This research highlights potential local and ocean-based drivers of glacial dynamics, and difficulties in separating the effects of global climate change from regional expressions of decadal-scale climate variability.

[1] We present a tree-ring based reconstruction of the Atlantic Multidecadal Oscillation (AMO) which demonstrates that strong, low-frequency (60–100 yr) variability in basin-wide (0–70°N) sea surface temperatures (SSTs) has been a consistent feature of North Atlantic climate for the past five centuries. Intervention analysis of reconstructed AMO indicates that 20th century modes were similar to those in the preceding ∼350 yr, and wavelet spectra show robust multidecadal oscillations throughout the reconstruction. Though the exact relationships between low-frequency SST modes, higher frequency (∼7–25 yr) atmospheric modes (e.g., North Atlantic Oscillation/Arctic Oscillation), and terrestrial climates must still be resolved, our results confirm that the AMO should be considered in assessments of past and future Northern Hemisphere climates.

For more than 60 years, RNA has been detectable in fixed cells and tissues by relatively specific staining methods. More recently, it has become possible to study RNA in unfixed, live cells. This review article describes how the intracellular dynamics and localization of RNA in vivo can be studied by microinjection of fluorescent RNA into cells- an approach we have termed Fluorescent RNA Cytochemistry. Depending on the particular RNA species under investigation, Fluorescent RNA Cytochemistry can operate as a "stain" to reveal intracellular sites at which a given RNA resides, or as a "tracer" to allow movements of a dynamically translocating RNA to be followed in the living cell. Several examples of Fluorescent RNA Cytochemistry are presented, collectively illustrating the range of applicability this approach offers in the toolbox of gene expression, studied as in vivo cell biology.

A method was developed to detect atrazine at a 0.05 ppb detection limit with a 10 ml_ sample volume. The herbicide Is extracted from 10 mL water with C18 solid-phase extraction and eluted into a reduction vessel with ethyl acetate. The sample is then evaporated to dryness under a stream of nitrogen and redlssolved for Injection Into a gas chromatograph/ mass spectrometer. Terbuthylazlne is used as Internal standard to compensate for extraction process variables. Standard curves have consistently shown correlation coefficients of 0.99 or better. Duplicate samples show a variance of 5% or less. Twelve samples can be extracted In about 1.5 h with

The role of the occupational health professional in determining fitness for duty is expanding. Proper determination of fitness for duty diffuses acute situations in the workplace. The occupational health professional's role is nonjudgmental and objective. This protocol protects the occupational health service from punitive actions.

An economical ground-water sampling system was constructed using readily available parts. The design is durable and easy to use in the field. Problems with cross contamination between wells are minimized; however, this sampling system is limited to shallow depths and is not appropriate when change in sample pressure would significantly affect the water chemistry.

Psoralen crosslinking of RNA–RNA intermolecular duplexes in sea urchin egg extracts reveals that some maternal poly(A)+ RNA molecules are complexed with U1 RNA, a cofactor in somatic nuclear pre-mRNA splicing. Reaction of egg extracts with a monoclonal antibody specific for U1 snRNP selects, in addition to Ul, RNAs that contain repeated sequences interspersed with single-copy elements. Antibodyselection experiments with nucleate and anucleate egg halves demonstrate that most of the Ul RNA–inter-spersed RNA complexes are cytoplasmic, as is the egg’s store of total Ul snRNP. These results raise the possibility that maternal interspersed RNAs include unprocessed pre-messenger RNA molecules in arrested complexes with splicing cofactors.

Heterogeneous nuclear RNA is normally complexed with a specific set of proteins, forming ribonucleoprotein particles termed hnRNP. These particles are likely to be involved in mRNA processing. We have found that the structure of hnRNP is profoundly altered during the heat shock response in Drosophila cultured cells. Although hnRNA continues to be synthesized at a near-normal rate during heat shock, its assembly into hnRNP is incomplete, as evidenced by a greatly decreased protein content of the particles in Cs2SO4 density gradients. RNA-protein cross-linking conducted in vivo (Mayrand and Pederson, Proc. Natl. Acad. Sci. U.S.A. 78:2208-2212, 1981) also reveals that hnRNA made during heat shock is complexed with greatly reduced amounts of protein. The block of hnRNP assembly occurs immediately upon heat shock, even before the onset of heat shock protein synthesis. Additional experiments reveal that hnRNP assembled normally at 25 degrees C subsequently disassembles during heat shock. The capacity for normal hnRNP assembly is gradually restored after heat-shocked cells are returned to 25 degrees C. Heat-shocked mammalian cells also show a similar block in hnRNP assembly. We suggest that incomplete assembly of hnRNP during heat shock leads to abortive processing of most mRNA precursors and favors the processing or export (or both) of others whose pathway of nuclear maturation is less dependent on, or even independent of, normal hnRNP particle structure. This hypothesis is compatible with a large number of previous observations.

In Drosophila, two nuclear proteins of approximately 26,000 and 14,000 molecular weight are recognized by a human autoimmune antibody for mammalian ribonucleoprotein (RNP) particles that contain U1 small nuclear RNA. The antibody-selected Drosophila RNP contains, in addition to these two proteins, a single RNA species that has been identified as U1 by hybridization with a cloned Drosophila U1 DNA probe. Small nuclear RNP isolated from human cells under the same conditions as used for Drosophila and selected by the anti-U1 RNP-specific antibody contains eight proteins, two of which are similar in molecular weight to the two Drosophila U1 RNP proteins. Thus, even though the nucleotide sequences of Drosophila and human U1 RNA are about 72% homologous, and the corresponding RNPs are both recognized by the same human autoantibody, Drosophila U1 RNP appears to have a simpler protein complement than its mammalian counterpart. The two Drosophila U1 RNA-associated proteins are synthesized at normal or slightly increased rates during the heat shock response and are incorporated into antibody-recognizable RNP complexes. This raises the possibility that U1 RNP is an indispensable nuclear element for cell survival during heat shock.

Activity in hydrogeology has increased dramatically in recent years. Position descriptions in want ads, published studies describing the working professional, and published descriptions of hydrogeology programs were examined to determine trends. The number of advertised positions for hydrogeologists is 10 times that of 5 years ago with the percentage of the total increasing from 50 to 75 in the private consulting field. The requirement for more than four years of experience has increased from 20 to 65 percent of the position descriptions. At least a master's degree was stipulated in over half of the position descriptions. Writing and management skills are stressed in recent ads. In general, the want ads parallel the profile of currently employed hydrogeologists. Educational programs meet most training requirements for the prospective professional except in the area of low-temperature geochemistry. Sixty percent of the positions advertised list skills in groundwater quality and/or contamination as importan...

The small nuclear RNAs U1, U2, U4, and U5 are cofactors in mRNA splicing and, like the pre-mRNAs with which they interact, are transcribed by RNA polymerase II. Also like mRNAs, mature U1 and U2 RNAs are generated by 3' processing of their primary transcripts. In this study we have investigated the in vitro processing of an SP6-transcribed human U2 RNA precursor, the 3' end of which matches that of authentic human U2 RNA precursor molecules. Although the SP6-U2 RNA precursor was efficiently processed in an ammonium sulfate-fractionated HeLa cytoplasmic S100 extract, the product RNA was unstable. Further purification of the processing activity on glycerol gradients resolved a 7S activity that nonspecifically cleaved all RNAs tested and a 15S activity that efficiently processed the 3' end of pre-U2 RNA. The 15S activity did not process the 3' end of a tRNA precursor molecule. As demonstrated by RNase protection, the processed 3' end of the SP6-U2 RNA maps to the same nucleotides as does mature HeLa U2 RNA.

This study compared the productions of 35 articulation impaired children obtained by picture elicited single word and connected speech sampling methods. Productions were compared on the basis of both the number and type of errors made under the two testing conditions. Data analyses revealed significant differences in the number and type of errors and the prevalent error patterns revealed by the two sampling methods. The implications are discussed.

This investigation was to determine whether data on irrigation-well registration forms as reported by well drillers can be used in hydrologic studies. Transmissivity maps were prepared for Hamilton County, Nebraska using only specific capacity values computed from yield and drawdown data, as reported on irrigation well-registration forms. On one map each control point was the mean of the transmissivity values calculated for all wells in a section. On a second map each control point was the mean of the means for four contiguous sections sharing a common corner. The second map was similar to that of a previously prepared map based on control point values computed from specific capacities of selected wells, test hole logs, and geologic interpretations. These results suggest that reported registration data for a given area have a normal population distribution. In situations where individual data are unverified the data base can be treated as samples of a population where the mean of the means of several samples for adjacent areas is representative of actual field conditions.

Transcription of the Bal I E restriction fragment of adenovirus DNA by RNA polymerase II in a HeLa cell extract produces a RNA transcript 1,712 nucleotides in length. This transcript contains the first two elements of the tripartite leader that, in vivo, is spliced onto the late mRNAs. We have found that this adenovirus 2 transcript forms a specific ribonucleoprotein complex (RNP) in this in vitro system. The RNP particle sediments in sucrose gradients as a monodisperse peak at 50 S and has a buoyant density of 1.34 g/cm3 in Cs2SO4, indicating the same 4:1 protein/RNA composition as native nuclear RNPs that contain pre-mRNA sequences (hnRNP). Moreover, the in vitro-assembled RNP is resistant to concentrations of NaCl that are known to dissociate nonspecific RNA-protein complexes. The adenovirus 2 transcript is precipitated by a monoclonal antibody for hnRNP core proteins. In addition, RNA-protein crosslinking of [alpha-32P]UTP-labeled transcript/RNP complexes reveals that the major proteins in contact with the RNA are the Mr 32,500-41,500 species known to be associated with hnRNA in vivo. These results demonstrate the in vitro assembly of a specific RNA polymerase II transcript into RNP. Moreover, because the 1,712-nucleotide adenovirus 2 transcript lacks poly(A) addition sites and because the leader sequences are not spliced appreciably in this in vitro system, it follows that RNP formation requires neither polyadenylylation nor splicing, nor is it sufficient to cause the latter.