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Abdominal distension (AD) occurs in pregnancy and is also commonly seen in patients with ascites from various causes. Because the abdomen forms part of the "chest wall," the purpose of this study was to clarify the effects of AD on ventilatory mechanics. Airway pressure, four (vertical) regional pleural pressures, and abdominal pressure were measured in five anesthetized, paralyzed, and ventilated upright pigs. The effects of AD on the lung and chest wall were studied by inflating a liquid-filled balloon placed in the abdominal cavity. Respiratory system, chest wall, and lung pressure-volume (PV) relationships were measured on deflation from total lung capacity to residual volume, as well as in the tidal breathing range, before and 15 min after abdominal pressure was raised. Increasing abdominal pressure from 3 to 15 cmH2O decreased total lung capacity and functional residual capacity by approximately 40% and shifted the respiratory system and chest wall PV curves downward and to the right. Much smaller downward shifts in lung deflation curves were seen, with no change in the transdiaphragmatic PV relationship. All regional pleural pressures increased (became less negative) and, in the dependent region, approached 0 cmH2O at functional residual capacity. Tidal compliances of the respiratory system, chest wall, and lung were decreased 43, 42, and 48%, respectively. AD markedly alters respiratory system mechanics primarily by "stiffening" the diaphragm/abdomen part of the chest wall and secondarily by restricting lung expansion, thus shifting the lung PV curve as seen after chest strapping. The less negative pleural pressures in the dependent lung regions suggest that nonuniformities of ventilation could also be accentuated and gas exchange impaired by AD.

Parametric scaling studies of radiofrequency and neutral beam heated currentless Heliotron E plasmas have been performed. The parametric local electron transport analyses show that the electron energy transport in electron cyclotron heating (ECH) plasmas is nearly of the same magnitude as the neoclassically predicted transport inside the 2/3 radius, while neutral beam injection (NBI) plasmas and plasmas in the ion cyclotron range of frequencies (ICRF) are dominated by anomalous electron transport in the entire region. Scaling studies on the global energy confinement time reveal that ECH, NBI and ICRF plasmas obey approximately identical scalings that are characterized by continuous power degradation and favourable positive density dependence. The global energy confinement time is thought to be affected by the anomalous transport in the peripheral plasma regions in the same way for ECH, NBI and ICRF plasmas although the core plasma properties – such as local electron transport – seem to be different for the individual plasmas.

A kinetic analysis of the global energy confinement time for neutral beam heated Heliotron E plasmas has been performed with a 1-D, time independent transport analysis code, PROCTR-Mod. From a regression analysis of a representative sample selection of the presented data sets, the global energy confinement time, , is found to scale as , where α = 0.53 ± 0.10, β = −0.71 ± 0.09, and γ = 0.35 ± 0.14; the error bar indicates the 95% confidence region deduced from the limited data points; is the line average electron density; Pheat[MW] is the neutral beam heating power; and B[T] is the vacuum magnetic field at the magnetic axis. The data analysis shows that the favourable density dependence partially offsets the unfavourable power dependence and that anomalous electron transport loss becomes dominant in the overall energy balance as the beam power and the plasma density are increased. An alternative scaling law is also presented, which is to fit by an 'offset linear' law, , where ζ = 1.1 ± 0.33 and η = 15.0 ± 1.9; ne[1014 cm−3] is the volume average electron density. The latter scaling is found to provide a better fit to the presented data sets, in spite of its simple form. The parametric scaling of the local electron thermal diffusivity, χe is also discussed on the basis of the kinetic analysis.

Recent studies of transport, magnetohydrodynamic stability, and divertor action on Heliotron E are summarized. A pellet injector and a new diagnostic system are developed. Moreover, the Heliotron g...

Fourteen beagles were used to determine the effects of fentanyl and midazolam as a premedicant for mask induction of anaesthesia with sevoflurane. The drugs were administered to each dog in a randomised cross-over design with a seven-day washout period between experiments. After a 15-minute equilibration period, a treatment consisting of fentanyl (10 mug/kg bodyweight) and midazolam (0.2 mg/kg) was given either intravenously or intramuscularly. Anaesthesia was then induced by the use of a facemask with sevoflurane in 100 per cent oxygen at a flow rate of 4 l/minute. Vaporiser settings were increased by 0.8 per cent at 15-second intervals until the value corresponding to 4.8 per cent sevoflurane was achieved. The time to the onset and cessation of involuntary movements, loss of the palpebral reflex, negative response to tail-clamp stimulation, and endotracheal intubation and cardiopulmonary variables were measured. Both the treatments with tentanyl and midazolam resulted in a shorter and smoother induction of anaesthesia than treatment with saline, and the cardiopulmonary changes were smaller and milder.

There are several important roles for the electron cyclotron resonance heating (ECRH) methods in helical-type plasma experimental devices. One is to produce high-temperature and high-density current-free plasmas. Another is additional heating of electrons and resulting ion heating through equipartition. Plasma temperature and current profile controls with highly localized heating become advanced techniques to improve plasma confinement and to suppress some MHD instabilities. ECRH is also an ultimate candidate of a steady-state plasma sustainment.

In recent years, advances in optical imaging methods have facilitated the visualization of events in the developing cortex. In particular, the introduction of DNA encoding fluorescent protein into cells of the embryonic brain allows the visualization of progenitor cells; slice preparations of the cortex then allow the monitoring of the behavior of transfected cells in the context of the living cerebral wall by time-lapse microscopy. Such approaches have provided substantial information about the patterns of neuronal migration. However, as these techniques label large numbers of cells in the ventricular zone (VZ), it is difficult to follow individual cell shape changes or cell behaviors within the VZ, where neuron production and initial migration take place. Here, we report a unique method using the photoconvertable fluorescent protein Kaede, which emits green fluorescence and shifts to emitting red fluorescence upon radiation with UV. Using this method, we were able to follow the behavior of a particular pair of daughter cells among neighboring Kaede-positive cells in the SVZ of mouse brain slices. The spindle shape progenitor divided into two multipolar-shaped daughter cells. The cell-cell borders of daughter cells were clearly visualized, and easily describe the position and distance between two or more cells. The photoconvertable property of Kaede offers a powerful cell marking tool to identify the precise morphology and migratory behaviors of individual cells within living cortical slices.

Demonstration of a long pulse or a steady state operation of ECH and sustainment of non‐collapsed plasma only by ECH is important in LHD from both the confinement device and the heating system engineering points of view. A gyrotron with a diamond output window is introduced and operated at the power level of 150 kW for more than 1 hour after modification of the cooling and evacuation system of the ECH transmission line. The power of about 110 kW injected into LHD is used to sustain the plasma with the electron density of 1.5 × 1018 m−3 and central temperature of more than 1.0 keV for 3900 sec. The gas puffing rate is carefully controlled so that the plasma density does not exceed the critical value above which the plasma collapsed for given injection power, magnetic configuration and wall condition of LHD. The results of gyrotron operation, transmission system modification for long pulse and optimizations of the magnetic field configuration of LHD and gas puffing for a given injection condition are discussed.

The Advanced Microwave Scanning Radiometer for EOS (AMSR-E) was developed and provided to NASA's EOS Aqua satellite by the National Space Development Agency of Japan (NASDA). AMSR-E is the modified version of AMSR onboard the Advanced Earth Orbiting Satellite-II (ADEOS-II, currently called the Midori-II). AMSR-E has been in operation since June 2002 and providing continuous data record. This paper presents the status of post-launch calibration and data evaluation of the instrument.

The Advanced Microwave Scanning Radiometer for EOS (AMSR-E) aboard the Advanced Earth Orbiting Satellite-II (ADEOS-II) and AMSR-E aboard the AQUA have an ability to observe sea surface temperature (SST) through clouds. An accuracy of observed SST is 0.64 C estimated by comparing with oceanic buoy's SST. Spatial resolution of two AMSRs' SST is about 50 km, which is determined by AMSR's antenna diameter.

Genes whose expression was up-regulated in low boron (B)-acclimated tobacco BY-2 (Nicotiana tabacum L. cv. Bright Yellow 2) cells, which had been selected under a low supply of B, were screened by the cDNA differential subtraction method. Thirteen genes were identified, including early salicylate-inducible glucosyltransferase, glutamine synthetase, glutathione S-transferase, and a pathogenesis-related protein, which might constitute a rescue system for oxidative damage. This indicates that B deficiency might impose cellular redox imbalance on the cells. Two of the 13 genes were induced within 30 min of B removal in the parent cells, indicating fast signal transfer from the cell walls to the cytoplasm.

Summary form only given, as follows. A tunnel kiln heated only by microwaves has been developed. The industrial ceramics are sintered continuously in the roller hearth. The minimum sintering time is an hour which is shorter than the time in the fastest conventional tunnel kiln with electric heaters. The maximum processing rate is 50 kg/hour. The dimensions of the tunnel are 0.3 m /spl times/ 0.3 m cross section and 6 m length of heating zone. The 60 magnetron generates 53 kW in total at 2.45 GHz. The thickness of the double layer thermal insulating wall varied to get a temperature gradient from 500 degC at the entrance to 1300 degC at the top zone along the tunnel axis. The work pieces were carried with the speed at which the temperature of them can synchronize to that of the inner most tunnel wall to ensure the adiabatic thermal insulation criteria. The work pieces would have body heating uniformly by microwave. The kiln will be applicable to many kinds of ceramics just by tuning up the speed or microwave power.

Summary form only given. The electric field is uniform in the tunnel. The kiln has a blanket with double layer thermal insulation. Varying the thermal insulator can create the temperature gradient. The inner layer is made of mulite base cement. The loss tangent of it is the order of 10/sup -2/ which is equivalent to the work pieces. The microwave reflects on the metallic wall of the tunnel repeatedly and electric field intensity is expected to be uniform in it. The outer layer is thermal insulator made of alumina/silica fiberboard. The thickness of it varied along the axis to increase the temperature of the wall as illustrated.

The confinement of high‐energy particles produced by ICRF heating was studied in LHD experimentally setting magnetic axis at 3.6m and 3.75m in two methods. It was concluded that the confinement of high‐energy particles with Rax=3.6 m was better than that with Rax=3.75m. Based on this result the long pulse experiments were conducted in the magnetic configuration of Rax=3.6m. The plasma was sustained for 150sec. The total input power reached 71MJ though the pulse length was limited by the collapse of plasma due to density increase. The second harmonic experiment was done also with Rax=3.6m. The stored energy increased and the particles with the high‐energy were observed.

We propose an automatic seal imprint verification system composed of a seal imprint extraction stage and a verification stage. It is assumed that each seal imprint is affixed on a bankcheck which has a background pattern and a signature. The extraction stage to separately extract the seal imprint and the signature from a background consists of 3 steps: the transformation of RGB color bankcheck image into HSV color space, the extraction of seal imprint and signature by clustering method in HSV color space, and isolated noise and baseline elimination. The seal imprint verification stage following the extraction stage is based on a method using global and local features. An experiment has been performed to examine the performance of the proposed system. Forty genuine imprints and forty-eight forged imprints on real Japanese bankchecks with various background patterns and signatures were used in the experiment. The experiment achieved as low a false-rejection error rate (Type I error rate) of 7.5% and a false-acceptance error rate (Type II error rate) of 0%.

The high power and steady state ICRF system for the LHD has been being developed and tested. The wide band transmitter having a double coaxial output cavity is constructed and tested. 1.2 MW CW operation was achieved. The water cooled transmission line and tuners have been tested successfully. The fast wave ICRF antenna of the LHD has been designed and the steady state test on the R&D antenna has been carried out.

The application of microwaves for sintering traditional ceramic materials has been studied at both 84 GHz and 2.45 GHz. The 2.45 GHz microwave radiation can sinter traditional ceramics under the adequate thermal insulation and conventional microwave scattering methods can be used to reduce the standing mode effect.

The time-of-flight neutral particle measurement system1 has been installed on the 10-O port at the 2nd campaign. It has the capability of two-dimensional measurement with a larger viewing angle by using the pivot on the rectangular port, which is set inside of the 10-O mother port. The movable stage with the vertical scan mechanism rotates on the fan shaped stainless rails around the pivot. The sight lines of −2 to 32 degrees against the 10-O port surface makes the angle between the sight line and the magnetic axis of the standard configuration (3.6 m) of 95 to 35 in the 4th campaign. Therefore the analyzer can observe high-energy particles with these pitch angles. The higher scan speed of 0.17 degree/second than that in the last campaign (0.05 degree/s) can be obtained in this campaign. Precise spatial distribution of the high-energy particles can be expected because it enables the continuous scanning in the long discharge.

The small GTPase Rho1 has been shown to regulate the organization of the actin cytoskeleton and formation of the cell wall in the fission yeast Schizosaccharomyces pombe. Activity of Rho1 must be precisely regulated in vivo, since both increases and decreases in its activity affect cell growth and shape. Thus, it is important to clarify the mechanism by which the activity of Rho1 is regulated in vivo.Seven genes encoding putative GAPs, GTPase-activating proteins, for the function of the Rho-family proteins were isolated from S. pombe. After disruption of these genes, rga1+ was found to play important roles in cell growth and morphogenesis. In rga1 null cells, delocalized F-actin patches and extraordinary thickening of the cell wall and the septum were observed. On the other hand, over-expression of Rga1 produced shrunken or dumpy cells. The phenotype of the rga1 null cells or the Rga1-over-expressing cells was similar to that of cells containing abnormally high or low Rho1 activity, respectively. Moreover, direct association of Rga1 with Rho1 was shown. Rga1 was localized to the cell ends and septum where Rho1 is known to function.In S. pombe, Rga1 is involved in the F-actin patch localization, cell morphogenesis, regulation of septation, and cell wall synthesis, probably functioning as a GAP for the function of Rho1.

We treated a patient with intra-peritoneal recurrent tumor from colon cancer who responded completely to chemotherapy of combined low-dose Leucovorin (LV) and 5-fluorouracil (5-FU). The patient was a 75-year-old man. He underwent resection of the transverse colon, sigmoid colon and distal stomach for colon and gastric cancers. Nine months after the operation, his CEA level increased to 39.5 ng/ml and a CT scan revealed an intra-peritoneal tumor measuring about 5 cm. He received chemotherapy of 30 mg/day of LV that was injected in a bolus and 500 mg/day of 5-FU that was given i.v. by continuous infusion for 10 days. At the end of 2 cycles of this regimen, CT scan demonstrated complete tumor remission and the patient's CEA level decreased to normal level. After an additional cycle of this regimen, he received modulated chemotherapy combined with l-Leucovorin and 5-FU as an outpatient. However, after 3 months of treatment, a recurrent tumor was detected in the same portion and the first regimen was re-started for 5 days. After 4 cycles of treatment the tumor disappeared completely from a CT scan. It is important to investigate effective regimens that do not reduce the quality of life of the patient. This clinical experience suggests that a low-dose LV/5-FU therapy may be beneficial to patients with recurrent colon cancer. Further investigation is necessary to establish an effective regimen that can be given for a long period without adverse effects on quality of life.

We measured the activity of several acid hydrolases of cultured oligodendrocytes prepared from adult bovine brain white matter to clarify the biochemical basis of bovine oligodendrocytes in vitro. Lysosomal enzyme activities were assayed by using 4-methylumbelliferyl glycosides as substrates. Lysosomal enzyme activities became higher at 8-11 days in vitro (DIV) than 4 DIV. The enrichment in acid hydrolase specific activities in oligodendrocytes may be associated with lysosomal origin of myelin-like membranes.

Our previous studies have shown that acidic glycosphingolipid, ganglioside GM1 (GM1), is an endogenous regulator of high affinity nerve growth factor receptor, Trk, which is an essential factor for the normal development and differentiation of neuronal cells by forming a complex with Trk. GM1 is also known to be a major constituent of caveola or glycosphingolipid-enriched microdomain (GEM) of the plasma membrane. In order to study the effect of the glycosylation of Trk on the formation of GM1-Trk complex and subcellular distribution of this protein, we generated PC12 cells stably overexpressing Trk (PCtrk). Pretreatment of this stable clones with tunicamycin, a potent inhibitor of N-glycosylation, caused the appearance of unglycosylated Trk core protein. These unglycosylated Trk can hardly respond to its ligand, NGF. Sucrose density gradient analysis revealed that unglycosylated Trk core protein was recovered in high density fractions, whereas most of GM1 is present in low density fractions corresponding to caveola or GEM fractions. Moreover, these unglycosylated Trk proteins lose their ability to form a complex with GM1, although GM1 is present in the same high density fractions. These data strongly suggest that spatial segregation of GM1 from the Trk protein by the inhibition of the glycosylation of Trk might be an important molecular mechanism for the unresponsiveness to NGF. Moreover, the binding site of GM1 in the Trk protein might act as an important determinant for the normal trafficking of the Trk protein within the cells.

A long pulse discharge more than 2 minutes was achieved using Ion Cyclotron Range of Frequency (ICRF) heating only on the Large Helical Device (LHD). The final goal is a steady state operation (30 minutes) at MW level. A liquid stub tuner was newly invented to cope with the long pulse discharge. The liquid surface level was shifted under a high RF voltage operation without breakdown. In the long pulse discharge the reflected power was observed to gradually increase. The shift of the liquid surface was thought to be inevitably required at the further longer discharge. An ICRF heating system consisting of a liquid stub tuner was fabricated to demonstrate a feedback control impedance matching. The required shift of the liquid surface was predicted using a forward and a reflected RF powers as well as the phase difference between them. A liquid stub tuner was controlled by the multiprocessing computer system with CINOS (CHS Integration No Operating System) methods. The prime objective was to improve the performance of data processing and controlling a signal response. By employing this method a number of the program steps was remarkably reduced. A real time feedback control was demonstrated in the system using a temporally changed electric resistance.

Heating experiment using high-power ion cyclotron range of frequency (ICRF) waves has been carried out and remarkable results as an ICRF heating in helical system were obtained in LHD. The maximum ICRF power was 2.7 MW. Good he0ating condition was obtained when the ion cyclotron resonance layer was located near the saddle point of the magnetic field. In this condition, the wave frequency is 38.47 MHz and strength of magnetic field is 2.75 T. Most of ICRF power was absorbed by ions and behavior of high-energy ions was investigated in the various heating conditions. The stored energy more than 1 MJ was achieved by combination of the ICRF heating with NBI heating. Fundamental cyclotron heating was observed in the high stored energy experiment. Second harmonic heating regime was shown to be useful heating scheme in LHD. The length of the ICRF pulse was extended to more than two minutes in the low power operation.

Ion-cyclotron heating was applied to the Large Helical Device. When the proton-cyclotron resonance was near the saddle point of the magnetic field-strength plane, strong ion-cyclotron damping occurred. Under these conditions efficient plasma heating was achieved for more than one minute. A high-energy ion tail was observed, and the effective tail temperature was determined by a balance between the wave acceleration and the electron-drag relaxation. There was no apparent sign of particle orbit loss effect in the investigated density range of 0.8-1.3x10(19) m(-3).

Amyotrophic lateral sclerosis (ALS) is characterized by the selective degeneration of specific populations of cranial and spinal motor neurons. In this study, we examined the expression of the high affinity functional receptor for BDNF, TrkB, and assessed the functional state of TrkB by examining the level of phosphorylation on tyrosine residues in ALS spinal cords. The data showed that TrkB-immunoprecipitates prepared from cell-free lysates of ALS spinal cords by use of an anti-TrkB antibody contained much more TrkB protein than from controls. These TrkB proteins expressed in ALS spinal cords, however, are much less phosphorylated on tyrosine residues than those of controls. Moreover, RT-PCR analysis of TrkB mRNA in ALS spinal cords demonstrated that the expression of Trk B mRNA is also upregulated in ALS spinal cords compared with those of controls. These data strongly suggest that there exists an abnormality in TrkB-mediated intracellular signaling in ALS spinal cords and shed a light on the possibility of the therapeutic intervention by normalizing this intracellular signaling.

The major neuropathological feature in Parkinson's disease (PD) is severe degeneration of the dopamine (DA) neurons in the substantia nigra. Dopamine transporter (DAT) is an important protein in the regulation of DA neurotransmission. It has been reported that PD patients show a loss of DAT in striatum. We report here the findings of single photon emission computed tomography (SPECT) of the DAT with 2 beta-carboxymethoxy-3 beta-(4[123I]iodophenyl)tropane ([123I] beta-CIT) to investigate striatal DAT in 10 patients with PD, one patient with vascular parkinsonism (VP), and one patient with dystonia syndrome. Patients were evaluated using the Webster rating scale. Specific/nondisplaceable striatal binding ratio (V3") was obtained in each case. In PD patients, the uptake of [123I] beta-CIT was reduced, especially in the tail of putamen compared with caudate nucleus. Even in the early stage of PD, the uptake of beta-CIT was reduced not only in the severely affected side, but also in the mildly disturbed side of the brain. Putamen caudate ratio was generally low in PD patients. In VP patient, the uptake was reduced, but putamen caudate ratio was not decreased. V3" values showed significant correlation with the severity of clinical symptoms such as self-care, facies, posture, gait, speech, and Hoehn-Yahr's stage. On the other hand, V3" values were not significantly correlated with the degree of tremor, seborrhea, and duration of the illness. In conclusion, we found that SPECT of the [123I] beta-CIT is a useful method for the diagnosis in the patients presenting parkinsonism, and for the clinico-physiological estimation of parkinsonian symptoms such as self-care, facies, posture, gait, and speech.

In LHD discharges a significant enhancement of the global energy confinement has been achieved for the first time in a helical device with an edge thermal barrier, which exhibits a sharp gradient at the edge of the temperature profile. Key features associated with the barrier are quite different from those seen in tokamaks: (i) almost no change in particle (including impurity) transport, (ii) a gradual formation of the barrier, (iii) a very high ratio of the edge temperature to the average temperature, and (iv) no edge relaxation phenomenon. These features are very attractive in applying the thermal barrier to future reactor grade devices.

This paper gives an overview of new ICRF techniques developed on HT-7 superconducting tokamak during 1998 campaign, using the present two simple loop antennas, for wall conditioning/boron film deposition of the vessel and start-up of OH discharges: (1) The results show that He-plasma is reliably produced at the standard ICRH frequency (f=22.5 MHz), in a wide range of toroidal field, gas pressure and different RF power level. ICRF cleaning efficiency is about three times higher than that of glow discharge cleaning, this new technique has been used daily during experiments. (2) Start-up experimental results show that RF power was very effective to make the breakdown time less than 2 ms and helpful to assist the plasma start-up when the ICRF power reached 50 kW level. About 10%–20% reduction in the volt-second consumption was achieved according to different condition, evaluated at the beginning of the current flattop. (3) Boronization is carried out by using IBW antenna, the film depth is about 350 nm, and i...

The final goal of Ion Cyclotron Range of Frequency (ICRF) heating on the Large Helical Device (LHD) is characterized by its high power (up to 12MW) and by steady state operation (30 minutes). Initial ICRF heating experiments were carried out using a pair of loop antemas in the 2nd experimental campaign in 1998. The ICRF heating power was applied to an ECH-produced plasma at an RF power level of 300 kW for 0.2 seconds. An applied frequency of f=25.6 MHz was selected. A cyclotron resonance layer of hydrogen ions was located at the half minor radius during operation at B=1.5 T. A mode conversion layer of a He plasma with a minority of hydrogen ions was located between the magnetic axis and the last closed magnetic flux. The plasma stored energy was observed to increase to twice that of the ECH plasma (PECH=300 kW). The plasma stored energy of the ECH target plasma was 11-13 kJ at an average electron density of ne=8–9×1018 m−3 and a central electron temperature of Te0=400 eV. The plasma stored energy increase...

Ion Cyclotron Range of Frequency (ICRF) heating on the Large Helical Device (LHD) is characterized by high power (up to 12MW) and by steady-state operation (30 minutes). Research and development for ICRF heating have been carried out in recent years. A newly developed liquid stub tuner has demonstrated highly reliable performance as a stub tuner; it has withstood 63kV for 10 seconds and 50kV for 30 minutes. The liquid surface level could be shifted under high RF voltage without breakdown. A liquid impedance matching system has been designed and fabricated for ICRF heating on the LHD. This system consists of a liquid stub tuner and a liquid phase shifter. The liquid phase shifter was constructed by connecting two liquid stub tuners in a U-shaped configuration. An impedance matching can be acquired in a wide frequency range, i.e., 25–95MHz by selecting the length of 4m for the liquid stub tuner. At some frequencies, it was a problem that the RF voltage at the phase shifter became higher than that between the RF antenna and the impedance matching system. However, this difficulty has been resolved by adopting the use of asymmetrical heights of liquid surface levels at the U-shaped liquid phase shifter.

The simultaneous administration of nerve growth factor (NGF) has been found to prevent experimental neuropathies induced by anti-cancer drugs such as cisplatin, vincristine and taxol. However, it is clinically important to know whether NGF is beneficial once the neuropathy is already manifest. We established a bioassay system to examine the preventive effects of NGF in various treatment schedules. NGF significantly prevented the inhibition of neurite outgrowth by vincristine and taxol regardless of treatment schedules. The pre-treatment and co-treatment schedules were effective against cisplatin, but the post-treatment schedule was not. With regard to the neurite and nerve cell population densities, only the cisplatin group treated with NGF showed lower values than the control. These results indicate that NGF-treatment is effective for the toxic sympathetic nerve injury induced by vincristine and taxol regardless of the treatment schedule, but is not protective against cisplatin-induced nerve cell injury.

An 18-year-old female had common cold and insomnia in early March 1987. Later, abnormal speech and behavior, emotional incontinence, anorexia and consciousness disturbance appeared. On March 19, she was admitted to our hospital in semi-comatose state. Myoclonus-like movement on hands was observed, and epileptic attacks with tonic and clonic convulsions occasionally occurred. There were no neurological findings that suspected cerebral focal lesions. The respiration was assisted through tracheal intubation. Laboratory examinations showed inflammatory reactions (CRP+2, WBC 10,600) and transient high levels serum CK (6,215 IU). As she had bradycardia (30-40/min) with complete AV block on ECG, the pacemaker was implanted. The complication of myocarditis was suspected. EEG showed bilateral slow waves (3-6Hz), dominantly in frontal areas. Brain CT and CSF examinations were normal. After the combined administration of ara-A, dexamethasone and anti-convulsant, the consciousness level was recovered within a month. The serum antibody against coxsackie virus B4 alone was significantly increased. We concluded that coxsackie virus B4 caused acute encephalitis with mental symptoms and myocarditis with AV block. Recently, cytomegalovirus was reported to be the causative virus in a young female with non-HSV encephalitis who showed mental symptoms with good prognosis, but coxsackie virus B4 should also be considered as one of the causative viruses.

To evaluate cardiopulmonary effects of sevoflurane (Sevo), compared with halothane (Hal), enflurane (Enf), and isoflurane (Iso).24 healthy Beagles, randomly assigned to 4 groups of 6 dogs each.Dogs under spontaneous ventilation were anesthetized with Sevo, Hal, Enf, or Iso. After minimum elveolar concentration (MAC) of each anesthetic was determined, anesthesia was maintained at light (1 MAC), moderate surgical (1.5 MAC), and deep (2 MAC), stages and cardiopulmonary variables at conscious state (baseline) and each anesthesia stage were measured.In dogs of the Sevo group, heart rate increased significantly from the baseline value at all anesthesia stages. Systemic vascular resistance during Sevo anesthesia decreased gradually with increasing anesthesia stage, which was accompanied by dose-dependent decreases in systolic, mean, and diastolic arterial blood pressures. At 1.5 and 2 MAC Sevo, stroke index decreased slightly but significantly from the baseline value; however, cardiac index was unchanged because of the significant increase in heart rate. Respiratory rate decreased significantly at 2 MAC from that at 1 MAC Sevo. Tidal volume and dead space-to-tidal volume ratio were unchanged at all anesthesia stages of Sevo, resulting in significantly decreased expired and alveolar ventilation at 2 MAC, compared with values at 1 and 1.5 MAC Sevo. PaCO2 increased and pHa decreased significantly, depending on anesthesia stage; PaO2, increased significantly from baseline values, and remained constant because of inhalation of 100% O2.Cardiovascular effects of Sevo were greater than those of Hal, similar to those of Iso, and less than those of Enf. Respiratory effects of Sevo were similar to those of Iso at all anesthesia stages, greater than those of Hal at 2 MAC, and less than those of Enf at 1.5 and 2 MAC. Up to the moderate surgical anesthesia stage, Sevo can be used safely in dogs undergoing spontaneous ventilation.

The steady-state operation of high-performance plasmas in the Large Helical Device (LHD) has progressed since the 2010 IAEA Conference in Korea by means of a newly installed ion cyclotron heating (ICH) antenna (HAS antenna) and an improved electron cyclotron heating (ECH) system. The HAS antenna can control the launched parallel wave number and heat the core plasma efficiently in the case of dipole mode operation. Understanding of the physics and technology of wave heating, particle and heat flow balances, and plasma?wall interactions in LHD has also improved. The heating power of steady-state ICH and ECH exceeded 1?MW and 500?kW, respectively, and a higher density helium plasma with minority hydrogen ions was maintained using the HAS antenna and new 77?GHz gyrotrons. As a result, plasma performance improved, e.g. electron temperature of more than 2?keV at a density of more than 2???1019?m?3 became possible for more than 1?min. Heat flow balance and particle flux balance of steady-state operation were evaluated. Particle balance analysis indicated that externally fed helium and hydrogen particles were mainly absorbed by the chamber wall and divertor plates, even after the 54?min operation.

We will describe the capabilities of a folded waveguide (FWG) antenna and its application to ion Bernstein wave (IBW) heating in the Large Helical Device (LHD). LHD is scheduled to begin plasma operation in 1998. We are currently developing a FWG antenna to launch IBW to both produce and sustain the LHD plasma. We have fabricated a mockup FWG antenna to obtain the fundamental RF characteristics. The mockup FWG can produce various wave numbers parallel to the magnetic field line with either polarization plates or shorting bars in the antenna aperture. Preliminary results show a sharply defined wave number spectrum along the magnetic field line, which could provide efficient core electron heating. This type of direct electron heating is superior to fast wave minority heating, because it can avoid fast orbit losses of deeply trapped high energy ions.

Ion cyclotron range of frequency (ICRF) heating is currently in use on CHS and W7‐AS and is a major element of the heating planned for steady state helical devices. In helical devices, the lack of a toroidal current eliminates both disruptions and the need for ICRF current drive, simplifying the design of antenna structures as compared to tokamak applications. However the survivability of plasma facing components and steady state cooling issues are directly applicable to tokamak devices. Results from LHD steady state experiments should be available on a time scale to strongly influence the next generation of steady state tokamak experiments. The helical plasma geometry provides challenges not faced with tokamak ICRF heating, including the potential for enhanced fast ion losses, impurity accumulation, limited access for antenna structures, and open magnetic field lines in the plasma edge. The present results and near term plans provide the basis for steady state ICRF heating of larger helical devices. An a...

Lysosomal storage disease is one of the inborn errors of metabolism caused by a deficiency of lysosomal acid hydrolase activity. We describe here the details of screening methods for the diagnosis of this disorder. It is definitely important to perform both enzyme assay of acid hydrolases and the detection of accumulated materials in patient's tissues. Leukocytes (lymphocytes), serum or plasma, and cultured skin fibroblasts are commonly used as the enzyme source for the assay. Although most lysosomal storage diseases can be diagnosed using leukocytes as the enzyme source, enzymatic activities of beta-glucosidase and sialidase in leukocytes are sometimes normal even in patients. At present, the most reliable enzyme source is considered to be cultured skin fibroblasts. Nevertheless, we should remind that we cannot detect a deficiency of galactocerebroside beta-galactosidase activity even using fibroblasts, if we use synthetic substrate. Natural substrates should be employed for the correct diagnosis and for the study of the nature of patient's enzyme. Deficiency of the enzymatic activity in patients should be confirmed by the demonstration of accumulated materials due to the enzyme defect in patient's tissues and urine. The accumulation of mucopolysaccharides and oligosaccharides in urine is obvious in patients with mucopolysaccharidoses and mucolipidoses, respectively. In case of sphingolipidoses, rectal biopsy specimen and blood could be a target of the investigation. In final, the choice of these screening methods should be made solely based on the detailed clinical manifestation of patients.

Seroconversion from hepatitis B e antigen (HBeAg) to anti-HBe antibody (anti-HBe) frequently occurs in hosts chronically infected with hepatitis B virus (HBV). Further, this phenomenon is related to a point mutation from guanine to adenine at nucleotide 83 in the precore region of HBV, which, converts codon 28 for tryptophan (TGG:W) to a translational, stop codon (TAG:X). Therefore, we decided to examine HBV in sera from patients for mutations in the precore region by a simple allele-specific oligonucleotide (ASO) probe method. Direct sequencing was first performed on DNA fragments amplified by the polymerase chain reaction in order to establish whether there were mutations in the precore region. Subsequently, specific DNA probes were applied to detection of mutations in the precore region. Subsequently, specific DNA probes were applied to detection of mutations in the precore gene. Five unknown mutations (I10N, C12W, C14S, V17F and A19D), three known mutations (I9V, W28X and G29D) and for novel nucleotide insertions were identified in anti-HBe positive sera. By using seven nonradioactive probes, we could determine the mutations at codons 9, 28 and 29 in anti-HBe positive sera. The W28X mutation was found in anti-HBe positive but not in any of HBeAg positive sera. Meanwhile, wild-type strains of HBV were detectable in sera from patients who were positive to HBeAg or anti-HBe. This ASO probe assay could determine in a few days the mutations in the precore region of HBV, especially including the defect to prohibit the synthesis and secretion of HBeAg.

Since the successful first plasma generation in the middle of 2008, three experimental campaigns were successfully made for the KSTAR device, accompanied with a necessary upgrade in the power supply, heating, wall-conditioning and diagnostic systems. KSTAR was operated with the toroidal magnetic field up to 3.6 T and the circular and shaped plasmas with current up to 700 kA and pulse length of 7 s, have been achieved with limited capacity of PF magnet power supplies. The mission of the KSTAR experimental program is to achieve steady-state operations with high performance plasmas relevant to ITER and future reactors. The first phase (2008–2012) of operation of KSTAR is dedicated to the development of operational capabilities for a super-conducting device with relatively short pulse. Development of start-up scenario for a super-conducting tokamak and the understanding of magnetic field errors on start-up are one of the important issues to be resolved. Some specific operation techniques for a super-conducting device are also developed and tested. The second harmonic pre-ionization with 84 and 110 GHz gyrotrons is an example. Various parameters have been scanned to optimize the pre-ionization. Another example is the ICRF wall conditioning (ICWC), which was routinely applied during the shot to shot interval. The plasma operation window has been extended in terms of plasma beta and stability boundary. The achievement of high confinement mode was made in the last campaign with the first neutral beam injector and good wall conditioning. Plasma control has been applied in shape and position control and now a preliminary kinetic control scheme is being applied including plasma current and density. Advanced control schemes will be developed and tested in future operations including active profiles, heating and current drives and control coil-driven magnetic perturbation.

In six patients with advanced pancreatic carcinoma, TIL and tumour-draining lymphocytes (TDL) were isolated from primary pancreatic tumour and regional lymph nodes. In comparison with TDL and peripheral blood lymphocytes (PBL), TIL contained a comparatively higher percentage of TCR gamma delta+ cells, although they were still a small fraction. By 2 weeks culture with rIL-2 and immobilized OKT-3 antibody, the TCR gamma delta+ cells in TIL were preferentially expanded at the early culture periods, although it was temporary. In four cases, the TCR gamma delta+ and CD8+ TCR alpha beta+ TIL were separated by negative sorting using flowcytometry. All the TCR gamma delta+ TIL were CD4-, CD8- (double negative), and one of the TIL lines was mostly composed of delta TCS1+ cells, while the others were delta TCS1-. In comparison with CD8+ TCR alpha beta+ TIL, all the TCR gamma delta+ TIL exhibited much stronger lytic activity against freshly isolated autologous pancreatic cancer cells. However, all the gamma delta+ TIL also exhibited a strong non-MHC-restricted cytotoxicity, and there was no correlation between the lytic pattern and the percentage of delta TCS1+ cells. These data suggest that the TCR gamma delta+ T cells can proliferate vigorously in a certain condition, and if successfully expanded in vitro they might be helpful material for effective adoptive immunotherapy.