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96 results on '"Suzuki, Seigo"'

52. Highly sensitive multiplex protein detection by droplet‐free digital ELISA.

Author

Akama, Kenji, Shirai, Kentaro, and Suzuki, Seigo

Subjects
ENZYME-linked immunosorbent assay, IMMUNOGLOBULINS, BIOSENSORS, ANTIGENS, IMMUNOASSAY
Abstract

Digital enzyme‐linked immunosorbent assay (ELISA) is a single molecule counting technology and is one of the most sensitive immunoassay methods. The key aspect of this technology is to concentrate enzyme reaction products from a single target molecule in femtoliter droplets. On the other hand, we have developed a novel Digital ELISA that does not require droplets; instead, enzyme reaction products are concentrated using a tyramide signal amplification system. In this study, we present a method that enables the multiplex detection of proteins based on Droplet‐free Digital ELISA. First, two types of paramagnetic beads immobilized with antibodies specific to different target proteins were incubated with a sample solution, and target proteins were captured on their specific beads. Then, these beads were labeled with horseradish peroxidase (HRP), and tyramide substrate reacted with HRP on beads, resulting in products of this reaction deposited on those beads. This signal amplification on beads makes it possible to count the number of labeled beads digitally. We used this approach to simultaneously detect IL‐6 and HBs Ag with single molecule resolution. The obtained limit of detections were 0.1 pg/mL and 0.013 IU/mL, respectively. Our method has potential applications in simple in vitro diagnostic systems for simultaneous and high‐sensitive detection of protein biomarkers. [ABSTRACT FROM AUTHOR]

Published
2019
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53. Bioinspired structural transition of synthetic polymers through biomolecular ligand binding.

Author

Suzuki, Seigo, Sawada, Toshiki, Ishizone, Takashi, and Serizawa, Takeshi

Subjects
*POLYMERIZATION, *LIGAND binding (Biochemistry), *MOLECULAR structure
Abstract

Inspired by ligand binding-triggered structural transition of biopolymers in living systems, we demonstrated the structural transition of thermoresponsive poly(N-isopropylacrylamide) through specific ligand binding of artificially evolved peptides to the polymer. [ABSTRACT FROM AUTHOR]

Published
2018
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58. Regional control after concomitant chemoradiotherapy without planned neck dissection in node-positive head and neck squamous cell carcinomas

Author

Sakashita, Tomohiro, primary, Homma, Akihiro, additional, Oridate, Nobuhiko, additional, Suzuki, Seigo, additional, Hatakeyama, Hiromitsu, additional, Kano, Satoshi, additional, Mizumachi, Takatsugu, additional, Onimaru, Rikiya, additional, Tsuchiya, Kazuhiko, additional, Yasuda, Koichi, additional, Shirato, Hiroki, additional, and Fukuda, Satoshi, additional

Published
2013
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62. Clinical study of T3 laryngeal carcinoma

Author

Mizumachi, Takatsugu, primary, Oridate, Nobuhiko, additional, Homma, Akihiro, additional, Sakashita, Tomohiro, additional, Kano, Satoshi, additional, Hatakeyama, Hiromitsu, additional, Suzuki, Seigo, additional, and Fukuda, Satoshi, additional

Published
2012
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64. Clinical Analysis of Malignant Submandibular Tumor

Author

Kitao, Kyoko, primary, Homma, Akihiro, additional, Oridate, Nobuhiko, additional, Suzuki, Seigo, additional, Suzuki, Fumiyuki, additional, Hara, Toshihiro, additional, Kano, Satoshi, additional, Mizumachi, Takatsugu, additional, Taki, Shigenari, additional, Inamura, Naoya, additional, and Fukuda, Satoshi, additional

Published
2011
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65. HER2 expression in salivary duct carcinoma

Author

Suzuki, Takayoshi, primary, Kano, Satoshi, additional, Oridate, Nobuhiko, additional, Honma, Akihiro, additional, Suzuki, Seigo, additional, Hatakeyama, Hiromitsu, additional, Mizumachi, Takatsugu, additional, Furusawa, Jun, additional, Sakashita, Tomohiro, additional, and Fukuda, Satoshi, additional

Published
2011
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71. Importance of comorbidity in hypopharyngeal cancer

Author

Homma, Akihiro, primary, Sakashita, Tomohiro, additional, Oridate, Nobuhiko, additional, Suzuki, Fumiyuki, additional, Suzuki, Seigo, additional, Hatakeyama, Hiromitsu, additional, Mizumachi, Takatsugu, additional, Taki, Shigenari, additional, and Fukuda, Satoshi, additional

Published
2009
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72. Salvage surgery for recurrent cases of laryngeal or hypopharyngeal cancer following concurrent chemoradiotherapy

Author

Suzuki, Fumiyuki, primary, Homma, Akihiro, additional, Oridate, Nobuhiko, additional, Suzuki, Seigo, additional, Mizumachi, Takathugu, additional, Kanou, Satoshi, additional, Taki, Shigenari, additional, Inamura, Naoya, additional, Onimaru, Rikiya, additional, Hasegawa, Masakazu, additional, Shirato, Hiroki, additional, Furuta, Yasushi, additional, and Fukuda, Satoshi, additional

Published
2009
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78. Differentially expressed genes associated with CIS‐diamminedichloroplatinum (II) resistance in head and neck cancer using differential display and CDNA microarray

Author

Higuchi, Eisaku, primary, Oridate, Nobuhiko, additional, Furuta, Yasushi, additional, Suzuki, Seigo, additional, Hatakeyama, Hiromitsu, additional, Sawa, Hirofumi, additional, Sunayashiki‐Kusuzaki, Kousaku, additional, Yamazaki, Ken‐ichi, additional, Inuyama, Yukio, additional, and Fukuda, Satoshi, additional

Published
2002
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94. Differentially expressed genes associated with <TOGGLE><SC>CIS</SC></TOGGLE>-diamminedichloroplatinum (II) resistance in head and neck cancer using differential display and <TOGGLE><SC>C</SC></TOGGLE>DNA microarray

Author

Higuchi, Eisaku, Oridate, Nobuhiko, Furuta, Yasushi, Suzuki, Seigo, Hatakeyama, Hiromitsu, Sawa, Hirofumi, Sunayashiki-Kusuzaki, Kousaku, Yamazaki, Ken-ichi, Inuyama, Yukio, and Fukuda, Satoshi

Abstract

The mechanism by which cancer cells become resistant to cis-Diamminedichloroplatinum (II) (cDDP) is not completely understood. To investigate the molecular markers involved in the cDDP resistance, we compared the gene expression profiles between a head and neck squamous cell carcinoma (HNSCC) line sensitive to cDDP and its cDDP-resistant variant. Both a fluorescent differential display and a cDNA microarray analysis were applied to distinguish the gene profiles between KB, a human HNSCC line, and its cDDP-resistant variant (KB/cDDP). These results were confirmed by Northern blot analysis. One up-regulated gene, glycoprotein hormone α-subunit, and two down-regulated genes coding membrane proteins, human folate receptor and tumor-associated antigen L6, were identified in KB/cDDP cells. Our findings suggest that development of the cDDP-resistant phenotype is accompanied by alternations of gene expression including a glycoprotein hormone and membrane proteins. These gene products could be new molecular markers for resistance to cDDP. © 2003 Wiley Periodicals, Inc. Head Neck 25: 187–193, 2003

Published
2003
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96. Voice-related quality of life after treatment of laryngeal cancer.

Author

Oridate N, Homma A, Suzuki S, Nakamaru Y, Suzuki F, Hatakeyama H, Taki S, Sakashita T, Nishizawa N, Furuta Y, and Fukuda S

Subjects
Aged, Aged, 80 and over, Carcinoma, Squamous Cell complications, Carcinoma, Squamous Cell pathology, Chemotherapy, Adjuvant, Female, Humans, Laryngeal Neoplasms complications, Laryngeal Neoplasms pathology, Laryngectomy, Laser Therapy, Male, Middle Aged, Radiotherapy, Adjuvant, Surveys and Questionnaires, Voice Disorders classification, Voice Disorders psychology, Carcinoma, Squamous Cell therapy, Laryngeal Neoplasms therapy, Quality of Life, Voice Disorders etiology
Abstract

Objective: To determine patient-perceived voice-related quality of life in patients treated with various methods based on the results of Voice-Related Quality of Life (VRQOL) and Voice Handicap Index-10 (VHI-10) questionnaires., Design: The VRQOL and VHI-10 questionnaires., Setting: University hospital., Patients: One hundred thirty-seven patients who had received definitive treatment of laryngeal cancer were followed-up at Hokkaido University Hospital, Sapporo, Japan, and were alive with no evidence of malignancy at the time of the survey., Main Outcome Measure: Patient-perceived voice-related quality of life based on the results of the VRQOL and VHI-10 questionnaires., Results: The mean VRQOL scores for patients who had undergone radiotherapy (n = 63), chemoradiotherapy (n = 29), laser surgery (n = 14), or total laryngectomy (n = 27) as final treatment of laryngeal cancer were 92.6, 92.9, 85.5, and 68.4, respectively; the mean VHI-10 scores were 2.87, 2.34, 5.43, and 11.26, respectively., Conclusion: The VRQOL and VHI-10 questionnaires are important in judging the overall effectiveness of treatment options for laryngeal cancer.

Published
2009
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